March 31, 2017 Journal article Open Access

EFFECT OF HYDROGEN SULFIDE SYNTHESIS ENZYME INHIBITION BY HISTOPATHOLOGY IN PSYCHOLOGICAL STRESS-INDUCED ULCERS IN MICE

Namrata Gupta1*, Dr. Pankaj V. Dixit2, Dr. Ashish Dixit3

Objective: Effect of Hydrogen Sulfide Synthesis Enzyme Inhibition by histopathology in Psychological Stress-Induced Ulcers in Mice. Material and methods: Acute ulcers were induced in Swiss albino mice by using stress. The experimental groups consists of the following 2 groups: sender, responder groups. Sender animals received a foot shock of 10 sec duration at intervals of 50 sec for 3 hour. The electrical current for the shock 1.6 mA to 2.0 mA per hour for 3 day sender animals are changed daily. On day-3, after completing the foot-shock period, the responders were sacrificed, and their stomach were removed. Drug (O- carboxy methyl hydroxylamine hemihydrochloride) were administered intraperitoneally with low (2mg/kg), medium (5 mg/kg), high (10 mg/kg) dose daily for 3 days respectively, 30 min before the shock period. Resulted: The proper development of ulcer with an average ulcer index of 5 mg/kg was found to be 33.42 which is significant with respect to control group. CBS at a dose of 5 mg/kg significantly increased the ulcer formation, which was corroborated by histopathology studies. Conclusion: The present study that observed inhibition of H2S synthesis blocker exacerbated the psychological stress induced ulcer implicacy an important role of H2S in regulator of stress.