Published December 18, 2016 | Version v1
Journal article Open

Clinical observation of dogs serologically positive for the potentially zoonotic Ehrlichia Canis in the Philippines

Creators

  • 1. University of the Philippines-Cebu
  • 2. College of Veterinary Medicine, Southwestern University
  • 3. Makati Dog and Cat Hospital

Description

Canine Ehrlichiosis is a disease with worldwide distribution caused by the potentially zoonotic Ehrlichia canis, a Gram negative intra-cellular pathogen transmitted by the Rhipicephalus sanguineus tick. Most studies have reported the epidemiological distribution of E. canis, but information on the clinical signs and hematological values were limited and retrospectively gathered. The present study endeavored to prospectively document the clinical profile of dogs that were serologically positive for E. canis in the Philippines. A total of 68 canine patients presented at the Makati Dog and Cat Hospital, Manila, Philippines were investigated. Inclusion criteria were history or presence of tick infestation, anemia, and/or thrombocytopenia. Blood samples were collected in EDTA tubes, and plasma samples were tested for E. canis using a commercial serological test kit. Clinical signs were recorded, and complete blood counts (CBC) were performed. Results revealed that 86.7% (59) were found serologically positive for E. canis. Most seropositive dogs had tick infestation, inappetence, and lethargy. Thrombocytopenia and anemia accompanied with normal WBC counts were the common hematologic findings. Statistical analyses revealed no significant differences in the hematological values of seropositive and seronegative dogs. Also, from the clinical signs, only lethargy was found to be significantly correlated with seropositivity (p value = 0.026). This study documents the clinical profile of dogs that were serologically positive for E. canis infection in the Philippines. Results are very useful to clinical practitioners who usually rely on clinical signs, CBC and commercial test kits for the diagnosis of Canine Ehrlichiosis

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References

  • Akhtardanesh, B., Ghanbarpour, R., & Blourizadeh, H. (2010). Serological evidence of canine monocytic ehrlichiosis in Iran. Comparative Clinical Pathology, 19(5), 469-474.
  • Alleman, R. & Sayler, K. (2010). Coinfections in canines with vector-borne diseases. DX Consult, 3(1), 18-21.
  • Baneth, G., Waner, T., Koplah, A., Weinstein, S., & Keysary, A. (1996). Survey of Ehrlichia canis antibodies among dogs in Israel. Veterinary Record, 138(1), 257-259.
  • Baticados, A. M., Baticados, W. N., Villarba, L. A., Carlos, E. T., Carlos, S. M. E. A. S., & Fajardo, P. V. (2011). PCR
  • assay and microscopy for examination of mixed Ehrlichia canis and Babesia species infection in bomb sniffing dogs and other canines in the National Capital Region, Philippines. Eurasian Journal of Veterinary Sciences, 27(2), 111-115.
  • Botma J, Mogongoa LF, Jaftha, AD & Van Rensburg J. W. (2012). Reference ranges for platelet indices using Sysmex XE-2100 blood analyser. Medical Technology South Africa, 26(2), 17-21.
  • Breitschwerdt, E. B., Hegarty, B. C., & Hancock, S. I. (1998). Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. Journal of Clinical Microbiology, 36(9), 2645-2651.
  • Buhles, W. C., Huxsoll, D. L., & Ristic, M. (1974). Tropical canine pancytopenia: Clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy, and challenge inoculation. Journal of Infectious Diseases, 130(4), 357-367.
  • Corales, J. M. I., Viloria, V. V., Venturina, V. M., & Mingala, C. N. (2014). The prevalence of Ehrlichia canis, Anaplasma platys and Babesia species. in dogs in Nueva Ecija, Philippines based on multiplex polymerase chain reaction (mPCR) assay. Annals of Parasitology, 60(4), 267-272.
  • Dantas-Torres, F. (2010). Biology and ecology of the brown dog tick, Rhipicephalus sanguineus. Parasites and Vectors, 3(1), 26-31.
  • de Caprariis, D., Dantas-Torres, F., Capelli, G., Mencke, N., Stanneck, D., Breitschwerdt, E. B., & Otranto, D. (2011). Evolution of clinical, haematological and biochemical findings in young dogs naturally infected by vector-borne pathogens. Veterinary Microbiology, 149(1), 206-212.
  • Frank, J. R., & Breitschwerdt, E. B. (1999). A retrospective study of ehrlichiosis in 62 dogs from North Carolina and Virginia. Journal of Veterinary Internal Medicine, 13(3), 194-201.
  • Gal, A., Harrus, S., Arcoh, I., Lavy, E., Aizenberg, I., Mekuzas-Yisaschar, Y., & Baneth, G. (2007). Coinfection with multiple tick-borne and intestinal parasites in a 6-week-old dog. The Canadian Veterinary Journal, 48(6), 619-622.
  • Gaunt, S. D., Beall, M. J., Stillman, B. A., Lorentzen, L., Diniz, P. P. V. P., Chandrashekar, R., & Breitschwerdt, E. B. (2010). Experimental infection and co-infection of dogs with Anaplasma platys and Ehrlichia canis: hematologic, serologic and molecular findings. Parasites and Vectors, 3(1), 33-42.
  • Groves, M. G., Dennis, G. L., Amyx, H. L., & Huxsoll, D. L. (1975). Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus). American journal of veterinary research, 36(7), 937-940.
  • Harikrishnan, T. J., Pazhanivel, N., & Chellappa, J. (2005). Concomitant Babesia gibsoni and Ehrlichia canis infection in a dog. Veterinarski arhiv, 75(6), 513-520.
  • Harrus, S., Alleman, A. R., Bark, H., Mahan, S. M., & Waner, T. (2002). Comparison of three enzyme-linked immunosorbant assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia canis. Veterinary Microbiology, 86(4), 361-368.
  • Higuchi, S., Kuroda, H., Hoshi, H., Kawamura, S. & Yasuda, Y. (1999). Development of Babesia gibsoni in the midgut of the nymphal stage of the tick, Rhipicephalus sanguineus. Journal of Veterinary Medical Science, 61(6), 697-699.
  • Inokuma, H., Ohno, K., & Yamamoto, S. (1999). Serosurvey of Ehrlichia canis and Hepatozoon canis Infection in Dogs in Yamaguchi Prefecture, Japan. Journal of Veterinary Medical Science, 61(10), 1153-1155.
  • McBride, J. W., Corstvet, R. E., Breitschwerdt, E. B., & Walker, D. H. (2001). Immunodiagnosis of Ehrlichia canis infection with recombinant proteins. Journal of Clinical Microbiology, 39(1), 315-322.
  • Mylonakis, M. E., Koutinas, A. F., Breitschwerdt, E. B., Hegarty, B. C., Billinis, C. D., Leontides, L. S., & Kontos, V. S. (2004). Chronic canine ehrlichiosis (Ehrlichia canis): a retrospective study of 19 natural cases. Journal of the American Animal Hospital Association, 40(3), 174-184.
  • Nyindo, M., Huxsoll, D. L., Ristic, M., Kakoma, I., Brown, J. L., Carson, C. A., & Stephenson, E. H. (1980). Cell-mediated and humoral immune responses of German Shepherd dogs and Beagles to experimental infection with Ehrlichia canis. American journal of veterinary research, 41(2), 250-254.
  • Ozata, F., & Ural, K. (2014). Thrombocyte indices in dogs infected with Ehrlichia canis and Anaplasma phagocytophilum. Revista MVZ Córdoba, 19(3), 4277-4288.
  • Perez, M., Bodor, M., Zhang, C., Xiong, Q., & Rikihisa, Y. (2006). Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Annals of the New York Academy of Sciences, 1078(1), 110-117.
  • Skotarczak, B. (2003). Canine ehrlichiosis. Annals of Agricultural and Environmental Medicine, 10(2), 137-142.
  • Tvedten, H., Lilliehöök, I., Hillström, A., & Häggström, J. (2008). Plateletcrit is superior to platelet count for assessing platelet status in Cavalier King Charles Spaniels. Veterinary Clinical Pathology, 37(3), 266-271.
  • Waner, T., Harrus, S., Bark, H., Bogin, E., Avidar, Y., & Keysary, A. (1997). Characterization of the subclinical phase of canine ehrlichiosis in experimentally infected beagle dogs. Veterinary Parasitology, 69(3), 307-317.
  • Waner, T. & Harrus, S. (2000). Canine monocytic ehrlichiosis. In L. E. Carmichael (Ed) Recent advances in canine infectious diseases. (pp 393-405). NY, USA: International Veterinary Information Service
  • Yamane, I., Conrad, P. A., & Gardner, I. A. (1993). Babesia gibsoni infections in dogs. Journal of Protozoology Research, 3(4), 111-125.
  • Ybanez, A. P. (2013). First report on Anaplasma platys infection in a dog in the Philippines. Iranian Journal of Veterinary Medicine, 7(3), 227-231.
  • Ybañez, A. P. (2014). First molecular evidence of Ehrlichia canis infection in dogs with probable disease relapse in the Philippines. Journal of Advanced Veterinary Research, 4(4), 184-188.
  • Ybañez, A. P., Perez, Z. O., Gabotero, S. R., Yandug, R. T., Kotaro, M., & Inokuma, H. (2012). First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu, Philippines. Ticks and Tick-borne diseases, 3(5), 288-293.
  • Ybañez, A. P., Ybañez, R. H. D., Villavelez, R. R., Malingin, H. P. F., Barrameda, D. N. M., Naquila, S. V., & Olimpos, S. M. B. (2016). Retrospective analyses of dogs found serologically positive for Ehrlichia canis in Cebu, Philippines from 2003 to 2014. Veterinary World, 9(1), 43-47.
  • Ybañez, A. P., Ybañez, R. H. D., Yokoyama, N., & Inokuma, H. (2015). RNA polymerase sub-unit β (rpoB) characterization of Ehrlichia canis detected from dogs and Rhipicephalus sanguineus ticks in Cebu, Philippines. Veterinarski Arhiv, 85(6), 601-608.
  • Zygner, W., Gójska, O., Rapacka, G., Jaros, D., & Wędrychowicz, H. (2007). Hematological changes during the course of canine babesiosis caused by large Babesia in domestic dogs in Warsaw (Poland). Veterinary Parasitology, 145(1), 146-151.