Dataset Open Access

Flow cytometry data from human iPSC-derived macrophages

Alasoo, Kaur; Rodrigues, Julia; Gaffney, Daniel

DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="" xmlns="" xsi:schemaLocation="">
  <identifier identifierType="DOI">10.5281/zenodo.234214</identifier>
      <creatorName>Alasoo, Kaur</creatorName>
      <affiliation>Wellcome Trust Sanger Institute</affiliation>
      <creatorName>Rodrigues, Julia</creatorName>
      <affiliation>Wellcome Trust Sanger Institute</affiliation>
      <creatorName>Gaffney, Daniel</creatorName>
      <affiliation>Wellcome Trust Sanger Institute</affiliation>
    <title>Flow cytometry data from human iPSC-derived macrophages</title>
    <subject>flow cytometry</subject>
    <subject>induced pluripotent stems cells</subject>
    <date dateType="Issued">2017-01-08</date>
  <resourceType resourceTypeGeneral="Dataset"/>
    <alternateIdentifier alternateIdentifierType="url"></alternateIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf"></relatedIdentifier>
    <rights rightsURI="">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
    <description descriptionType="Abstract">&lt;p&gt;Human induced pluripotent cells (iPSCs) were obtained from the HipSci project ( and differentiated into macrophages using an established protocol (van Wilgenburg, 2013). The genotype_id column of the flow_sample_metadata.txt file contains the canonical HipSci iPSC line name from which the macrophages were differentiated.&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Data acquisition&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;We used flow cytometry to measure the cell surface expression of three canonical macrophage markers: CD14, CD16 (FCGR3A/FCGR3B) and CD206 (MRC1). Macrophages were cultured in 10 cm tissue-culture treated plates and detached from the plates by incubation in 6 mg/ml lidocaine-PBS solution (Sigma L5647) for 30 minutes followed by gentle scraping. From each cell line we harvested between 300,000-500,000 cells. Detached cells were washed in media, centrifuged at 1200 rpm for 5 minutes and resuspended in flow cytometry buffer (2% BSA, 0.001% EDTA in D-PBS) and split into two wells of a 96-well plate. Nonspecific antibody binding sites were blocked by incubating cells with Human TruStain FcX (Biolegend) for 45 minutes and washing with flow cytometry buffer. Half of the cells were stained for 1 hour with the PE-isotype control (BD 555749) antibody. The other half of the cells were co-stained for 1 hour with following three antibodies: CD14-Pacific Blue (BD 558121), CD16-PE (BD 555407), CD206-APC (BD 550889). After staining, the cells were washed three times. Resuspended cells were filtered through cell-strainer cap tubes (BD 352235) and measured on the BD LSRFortessa Cell Analyzer.&lt;/p&gt;</description>
    <description descriptionType="Other">This work was supported by the Wellcome Trust grant #098051. K.A. was supported by a PhD fellowship from the Mathematical Genomics and Medicine programme from the Wellcome Trust.</description>
All versions This version
Views 244244
Downloads 3737
Data volume 1.5 GB1.5 GB
Unique views 230230
Unique downloads 2424


Cite as