Journal article Open Access

Impa (adenosine 5'-phosphorimidazolide) Synthesis

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    <subfield code="a">&lt;p&gt;This protocol is used to synthesize ImpA which is required to make the intermediate reaction product of RNA/DNA ligases.  This is desirable when the target molecule has a 5'-phosphate because use of an activated linker oligo allows ligation without ATP and avoids self-circularization of the molecule of interest.&lt;/p&gt;</subfield>
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