Planned intervention: On Thursday March 28th 07:00 UTC Zenodo will be unavailable for up to 5 minutes to perform a database upgrade.
Published December 30, 2016 | Version v1
Journal article Open

Impa (adenosine 5'-phosphorimidazolide) Synthesis

Creators

  • 1. ScientificProtocols.org

Description

This protocol is used to synthesize ImpA which is required to make the intermediate reaction product of RNA/DNA ligases. This is desirable when the target molecule has a 5'-phosphate because use of an activated linker oligo allows ligation without ATP and avoids self-circularization of the molecule of interest.

Files

protocol.md

Files (3.6 kB)

Name Size Download all
md5:d5cc1ce5e149f8dc36770784866d984c
3.6 kB Preview Download