Published December 30, 2016
| Version v1
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Impa (adenosine 5'-phosphorimidazolide) Synthesis
Description
This protocol is used to synthesize ImpA which is required to make the intermediate reaction product of RNA/DNA ligases. This is desirable when the target molecule has a 5'-phosphate because use of an activated linker oligo allows ligation without ATP and avoids self-circularization of the molecule of interest.
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protocol.md
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