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Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems

Harding, Rachel J.; Loppnau, Peter; Ackloo, Suzanne; Lemak, Alexander; Hutchinson, Ashley; Hunt, Brittany; Holehouse, Alex; Ho, Jolene; Fan, Lixin; Toledo-Sherman, Leticia; Seitova, Alma; Arrowsmith, Cheryl

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  author       = {Harding, Rachel J. and
                  Loppnau, Peter and
                  Ackloo, Suzanne and
                  Lemak, Alexander and
                  Hutchinson, Ashley and
                  Hunt, Brittany and
                  Holehouse, Alex and
                  Ho, Jolene and
                  Fan, Lixin and
                  Toledo-Sherman, Leticia and
                  Seitova, Alma and
                  Arrowsmith, Cheryl},
  title        = {{Design and characterisation of mutant and wild- 
                   type huntingtin proteins produced from a toolkit
                   of scalable eukaryotic expression systems}},
  note         = {{ACKNOWLEDGEMENTS We thank Dr. Xiaobing Zuo 
                   (Argonne National Laboratory) for expert support
                   with SAXS measurements, and acknowledge the use of
                   the SAXS Core facility of Center for Cancer
                   Research (CCR), National Cancer Institute (NCI)
                   which is funded by Frederick National Laboratory
                   for Cancer Research under contract
                   HHSN261200800001E and the intramural research
                   program of the NIH, NCI, CCR. The content of this
                   publication does not necessarily reflect the views
                   or policies of the Department of Health and Human
                   Services, nor does mention of trade names,
                   commercial products or organizations imply
                   endorsement by the US Government. This research
                   used 12-ID-B beamline of the Advanced Photon
                   Source, a U.S. Department of Energy (DOE) Office
                   of Science User Facility operated for the DOE
                   Office of Science by Argonne National Laboratory
                   under Contract No. DE-AC02-06CH11357. We also
                   acknowledge Dr. Pravin Mahajan who constructed the
                   pBMDEL vector. This research was supported by a
                   Huntington's Disease Society of America Berman
                   Topper Career Development Fellowship (RH), the
                   Natural Sciences and Engineering Research Council
                   of Canada (CHA) (grant RGPIN-2015-05939),
                   Huntington Society of Canada (CHA), CHDI
                   Foundation (LTS, CHA) and the SGC, a registered
                   charity (number 1097737) that receives funds from
                   AbbVie, Bayer Pharma AG, Boehringer Ingelheim,
                   Canada Foundation for Innovation, Eshelman
                   Institute for Innovation, Genome Canada through
                   Ontario Genomics Institute [OGI-055], Innovative
                   Medicines Initiative (EU/EFPIA) [ULTRA-DD grant
                   no. 115766], Janssen, Merck KGaA, Darmstadt,
                   Germany, MSD, Novartis Pharma AG, Ontario Ministry
                   of Research, Innovation and Science (MRIS),
                   Pfizer, São Paulo Research Foundation-FAPESP,
                   Takeda, and Wellcome. ASH is a postdoctoral fellow
                   in the laboratory of R.V. Pappu at Washington
                   University in St. Louis and is funded by the Human
                   Frontiers Science Program (grant RGP0034/2017).}},
  month        = dec,
  year         = 2018,
  doi          = {10.5281/zenodo.2169035},
  url          = {}
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