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Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


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{
  "description": "<p><strong>Project</strong> -&nbsp;Huntingtin structure-function open lab notebook.&nbsp;</p>\n\n<p><strong>Rationale </strong>-&nbsp;Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&nbsp;</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "name": "Harding, Rachel"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Hutchinson, Ashley"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Seitova, Alma"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "@type": "Person", 
      "name": "Edwards, Aled"
    }
  ], 
  "url": "https://zenodo.org/record/2040342", 
  "datePublished": "2018-08-04", 
  "keywords": [
    "huntingtin", 
    "Huntington's disease", 
    "HAP40", 
    "Protein purification"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/199d5b85-6dd2-4ada-914b-36bd8bf03cdc/Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells 20180804.docx", 
      "@type": "DataDownload", 
      "fileFormat": "docx"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/199d5b85-6dd2-4ada-914b-36bd8bf03cdc/Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells 20180804.pdf", 
      "@type": "DataDownload", 
      "fileFormat": "pdf"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.2040342", 
  "@id": "https://doi.org/10.5281/zenodo.2040342", 
  "@type": "Dataset", 
  "name": "Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells \u2013 2018/08/04"
}
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