Dataset Open Access

Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/199d5b85-6dd2-4ada-914b-36bd8bf03cdc/Large%20scale%20expression%20and%20purification%20of%20full-length%20huntingtin%20Q54%20with%20HAP40%20from%20baculoviral%20expression%20system%20production%20in%20sf9%20insect%20cells%2020180804.docx"
      }, 
      "checksum": "md5:4e5abcedc19bc9d4899a460b779b49e8", 
      "bucket": "199d5b85-6dd2-4ada-914b-36bd8bf03cdc", 
      "key": "Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells 20180804.docx", 
      "type": "docx", 
      "size": 562304
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/199d5b85-6dd2-4ada-914b-36bd8bf03cdc/Large%20scale%20expression%20and%20purification%20of%20full-length%20huntingtin%20Q54%20with%20HAP40%20from%20baculoviral%20expression%20system%20production%20in%20sf9%20insect%20cells%2020180804.pdf"
      }, 
      "checksum": "md5:1d578b216746ba0a554a9299adcfe5c0", 
      "bucket": "199d5b85-6dd2-4ada-914b-36bd8bf03cdc", 
      "key": "Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells 20180804.pdf", 
      "type": "pdf", 
      "size": 474588
    }
  ], 
  "owners": [
    19913
  ], 
  "doi": "10.5281/zenodo.2040342", 
  "stats": {
    "version_unique_downloads": 20.0, 
    "unique_views": 35.0, 
    "views": 45.0, 
    "downloads": 37.0, 
    "unique_downloads": 20.0, 
    "version_unique_views": 35.0, 
    "volume": 18436916.0, 
    "version_downloads": 37.0, 
    "version_views": 45.0, 
    "version_volume": 18436916.0
  }, 
  "links": {
    "doi": "https://doi.org/10.5281/zenodo.2040342", 
    "conceptdoi": "https://doi.org/10.5281/zenodo.2040341", 
    "bucket": "https://zenodo.org/api/files/199d5b85-6dd2-4ada-914b-36bd8bf03cdc", 
    "conceptbadge": "https://zenodo.org/badge/doi/10.5281/zenodo.2040341.svg", 
    "html": "https://zenodo.org/record/2040342", 
    "latest_html": "https://zenodo.org/record/2040342", 
    "badge": "https://zenodo.org/badge/doi/10.5281/zenodo.2040342.svg", 
    "latest": "https://zenodo.org/api/records/2040342"
  }, 
  "conceptdoi": "10.5281/zenodo.2040341", 
  "created": "2018-12-07T22:42:13.602876+00:00", 
  "updated": "2018-12-12T21:48:00.948076+00:00", 
  "conceptrecid": "2040341", 
  "revision": 6, 
  "id": 2040342, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.5281/zenodo.2040342", 
    "description": "<p><strong>Project</strong> -&nbsp;Huntingtin structure-function open lab notebook.&nbsp;</p>\n\n<p><strong>Rationale </strong>-&nbsp;Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&nbsp;</p>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells \u2013 2018/08/04", 
    "notes": "Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, S\u00e3o Paulo Research Foundation-FAPESP, Takeda, and Wellcome.", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "2040341"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "2040342"
          }
        }
      ]
    }, 
    "communities": [
      {
        "id": "labscribbles"
      }, 
      {
        "id": "openlabnotebooks"
      }, 
      {
        "id": "sgc-opennotebook"
      }
    ], 
    "keywords": [
      "huntingtin", 
      "Huntington's disease", 
      "HAP40", 
      "Protein purification"
    ], 
    "publication_date": "2018-08-04", 
    "creators": [
      {
        "orcid": "0000-0002-1134-391X", 
        "affiliation": "Structural Genomics Consortium, University of Toronto", 
        "name": "Harding, Rachel"
      }, 
      {
        "affiliation": "Structural Genomics Consortium, University of Toronto", 
        "name": "Hutchinson, Ashley"
      }, 
      {
        "affiliation": "Structural Genomics Consortium, University of Toronto", 
        "name": "Seitova, Alma"
      }, 
      {
        "affiliation": "Structural Genomics Consortium, University of Toronto", 
        "name": "Arrowsmith, Cheryl"
      }, 
      {
        "affiliation": "Structural Genomics Consortium, University of Toronto", 
        "name": "Edwards, Aled"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "type": "dataset", 
      "title": "Dataset"
    }, 
    "related_identifiers": [
      {
        "scheme": "doi", 
        "relation": "isVersionOf", 
        "identifier": "10.5281/zenodo.2040341"
      }
    ]
  }
}
45
37
views
downloads
All versions This version
Views 4545
Downloads 3737
Data volume 18.4 MB18.4 MB
Unique views 3535
Unique downloads 2020

Share

Cite as