Journal article Open Access

Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells.

Valeria V. Orlova; Francijna E. van den Hil; Sandra Petrus-Reurer; Yvette Drabsch; Peter ten Dijke; Christine L. Mummery


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    <subfield code="u">Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands</subfield>
    <subfield code="a">Yvette Drabsch</subfield>
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    <subfield code="u">Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands</subfield>
    <subfield code="a">Peter ten Dijke</subfield>
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    <subfield code="u">Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands. [2] Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands</subfield>
    <subfield code="a">Valeria V. Orlova</subfield>
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    <subfield code="a">Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells.</subfield>
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    <subfield code="a">&lt;p&gt;Human endothelial cells (ECs) and pericytes are of great interest for research on vascular development and disease as well as future therapy. This protocol describes the efficient generation of ECs and pericytes from human pluripotent stem cells (hPSCs) under defined conditions. Essential steps for hPSC culture, differentiation, isolation and functional characterization of ECs and pericytes are described. Substantial numbers of both cell types can be derived in only 2-3 weeks: this involves differentiation (10 days), isolation (1 day) and 4 or 10 days expansion of ECs and pericytes, respectively. We also describe two assays for functional evaluation of hPSC-derived ECs: (i) primary vascular plexus formation upon co-culture with hPSC-derived pericytes and (ii) incorporation in the vasculature of zebrafish xenografts &lt;em&gt;in vivo&lt;/em&gt;. These assays can be used to test the quality and drug sensitivity of hPSC-derived ECs and model vascular diseases with patient-derived hPSCs.&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&amp;nbsp;&lt;/p&gt;

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