18432
doi
10.1038/nprot.2014.102
oai:zenodo.org:18432
user-eu
Francijna E. van den Hil
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
Sandra Petrus-Reurer
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
Yvette Drabsch
Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands
Peter ten Dijke
Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands
Christine L. Mummery
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells.
Valeria V. Orlova
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands. [2] Department of Molecular Cell Biology, Cancer Genomics Centre, Leiden University Medical Center, Leiden, The Netherlands
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
hPSCs
Endothelial cells
Pericytes
<p>Human endothelial cells (ECs) and pericytes are of great interest for research on vascular development and disease as well as future therapy. This protocol describes the efficient generation of ECs and pericytes from human pluripotent stem cells (hPSCs) under defined conditions. Essential steps for hPSC culture, differentiation, isolation and functional characterization of ECs and pericytes are described. Substantial numbers of both cell types can be derived in only 2-3 weeks: this involves differentiation (10 days), isolation (1 day) and 4 or 10 days expansion of ECs and pericytes, respectively. We also describe two assays for functional evaluation of hPSC-derived ECs: (i) primary vascular plexus formation upon co-culture with hPSC-derived pericytes and (ii) incorporation in the vasculature of zebrafish xenografts <em>in vivo</em>. These assays can be used to test the quality and drug sensitivity of hPSC-derived ECs and model vascular diseases with patient-derived hPSCs. </p>
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Zenodo
2014-05-29
info:eu-repo/semantics/article
610374
user-eu
award_title=Pluripotent stem cell resources for mesodermal medicine; award_number=602423; award_identifiers_scheme=url; award_identifiers_identifier=https://cordis.europa.eu/projects/602423; funder_id=00k4n6c32; funder_name=European Commission;
1579542315.732412
2462703
md5:efcf27a6726b18baab039b79ab903ac6
https://zenodo.org/records/18432/files/Orlova_Nature_Protocols.pdf
public