Dataset Open Access

Maintenance of spatial gene expression by Polycomb-mediated repression after formation of a vertebrate body plan

Rougeot, Julien; Chrispijn, Naomi D.; Aben, Marco; Elurbe, Dei M.; Andralojc, Karolina M.; Murphy, Patrick J.; Jansen, Pascal WTC; Vermeulen, Michiel; Cairns, Bradley R.; Kamminga, Leonie M.

This dataset contains zebrafish (Danio rerio) raw RNA and ChIP sequencing data:

  • RNA-seq:
    • RNAseq_Wildtype_rep[12].fastq.gz: 2 biological replicates of single-end RNA-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • RNAseq_Wildtype_rep[3-6].fastq.gz 4 biological replicates of paired-end RNA-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
  • ChIP-seq:
    • lane1_MPZezh2WT-24hpf-Ezh2__R[12].fastq.gz: 1 sample of paired-end Ezh2 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • lane1_MPZezh2WT-24hpf-Rnf2__R[12].fastq.gz: 1 sample of paired-end Rnf2 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • lane1_MPZezh2WT-24hpf-H3K27me3__R[12].fastq.gz: 1 sample of paired-end H3K27me3 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • *MPZezh2WT-24hpf-H3K4me3*: 2 biological replicates of paired-end H3K4me3 ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-Ezh2-spikein-13277_R[12].fastq.gz: 1 sample of paired-end Ezh2 ChIP-seq data (with Drosophila H2Ay spike in) from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-H3K27A[cC]*: 2 biological replicates of paired-end H3K27ac ChIP-seq data from 24hpf wild-type (TU/TL background) whole embryo lysates
    • MPZezh2WT-24hpf-H3K27me3-spikein-13275_R[12].fastq.gz: 1 sample of paired-end H3K27me3 ChIP-seq data (with Drosophila H2Ay spike in) from 24hpf wild-type (TU/TL background) whole embryo lysates

The work was funded by the Innovative Research scheme of the Netherlands Organisation for Scientific research (www.nwo.nl, NOW-Vidi 864.12.009, L.M.K.), the Radboud University Nijmegen Medical Centre tenure track fellowship (www.radboudumc.nl, L.M.K.), the Howard Hughes Medical Institute and the Huntsman Cancer Institute core facilities (CA24014, B.R.C.), and the the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the NIH (T32HD007491, P.J.M.).
Files (25.5 GB)
Name Size
lane1_MPZezh2WT-24hpf-Ezh2__R1.fastq.gz
md5:bb2dac10c99054b8ac4be2bc7ea64c68
666.2 MB Download
lane1_MPZezh2WT-24hpf-Ezh2__R2.fastq.gz
md5:12e5b989e904ff8cba0123218cb7e0f8
695.1 MB Download
lane1_MPZezh2WT-24hpf-H3K27me3__R1.fastq.gz
md5:2950f374a20738bc758f1797659472ec
656.6 MB Download
lane1_MPZezh2WT-24hpf-H3K27me3__R2.fastq.gz
md5:895383778a1230938c0af1daa96131b4
685.1 MB Download
lane1_MPZezh2WT-24hpf-H3K4me3__R1.fastq.gz
md5:1d54e05179e98c60ce7ee88045f02888
1.1 GB Download
lane1_MPZezh2WT-24hpf-H3K4me3__R2.fastq.gz
md5:095b6376123cbf308ba0edbd5920db0d
1.2 GB Download
lane1_MPZezh2WT-24hpf-Rnf2__R1.fastq.gz
md5:b4377f3d58ecdcbc78773199d509fa17
849.4 MB Download
lane1_MPZezh2WT-24hpf-Rnf2__R2.fastq.gz
md5:9b2c6203c23f4ff30965d3ac8bb3be65
904.3 MB Download
MPZezh2WT-24hpf-Ezh2-spikein-13277_R1.fastq.gz
md5:2f4a2de786f744f2f02d81b0b6aa4853
1.0 GB Download
MPZezh2WT-24hpf-Ezh2-spikein-13277_R2.fastq.gz
md5:5291b46eb16b2ae691fffad8f6ad3e04
1.0 GB Download
MPZezh2WT-24hpf-H3K27Ac-13146_R1.fastq.gz
md5:bf27bc2cdcd204cb51b742c7b7491b14
1.7 GB Download
MPZezh2WT-24hpf-H3K27Ac-13146_R2.fastq.gz
md5:6c37a50d16b4b0242a04ce208cbd29eb
1.6 GB Download
MPZezh2WT-24hpf-H3K27AC-15059_R1.fastq.gz
md5:8b64029148c16007fd65cd0315b312a8
574.9 MB Download
MPZezh2WT-24hpf-H3K27AC-15059_R2.fastq.gz
md5:f8e008d29f175df64e4182aa5a53e86c
595.3 MB Download
MPZezh2WT-24hpf-H3K27me3-spikein-13275_R1.fastq.gz
md5:d9c78df5dd2d0e1f17374764014ab713
913.5 MB Download
MPZezh2WT-24hpf-H3K27me3-spikein-13275_R2.fastq.gz
md5:f92c249e0c01b393ecaa7ca68473b456
945.5 MB Download
MPZezh2WT-24hpf-H3K4me3-duplicate-13145_R1.fastq.gz
md5:024df6e71adbad67220f78021a02569f
1.5 GB Download
MPZezh2WT-24hpf-H3K4me3-duplicate-13145_R2.fastq.gz
md5:571231098d8e1de02b03ada1cbc64f55
1.6 GB Download
RNAseq_Wildtype_rep1.fastq.gz
md5:7284889d1475c85cadbebdb9f1d13326
1.4 GB Download
RNAseq_Wildtype_rep2.fastq.gz
md5:7b1e84230dde172d457c442cdf5caceb
1.6 GB Download
RNAseq_Wildtype_rep3_R1.fastq.gz
md5:d08c705dbcc56e3286bf982dda24853e
551.1 MB Download
RNAseq_Wildtype_rep3_R2.fastq.gz
md5:c45eadb7015a4444402c6e689a5dbd2d
574.8 MB Download
RNAseq_Wildtype_rep4_R1.fastq.gz
md5:38d587dda9cbbd91a8b1852ea628c75b
568.3 MB Download
RNAseq_Wildtype_rep4_R2.fastq.gz
md5:20ad61dd1c90b6c7be45371620bc981d
591.7 MB Download
RNAseq_Wildtype_rep5_R1.fastq.gz
md5:db5205876597848cbd9bcba973f42089
441.8 MB Download
RNAseq_Wildtype_rep5_R2.fastq.gz
md5:33abac506aab828908159864d50ecf26
457.9 MB Download
RNAseq_Wildtype_rep6_R1.fastq.gz
md5:fbe0fac04c79ac050cde33a78d2d01a4
525.9 MB Download
RNAseq_Wildtype_rep6_R2.fastq.gz
md5:8521bf2b215ae417300ee65c9ca40c16
549.4 MB Download
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