Journal article Open Access

Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model

Le Blon, Debbie; Guglielmetti, Caroline; Hoornaert, Chloé; Quarta, Alessandra; Daans, Jasmijn; Dooley, Dearbhaile; Lemmens, Evi; Praet, Jelle; De Vocht, Nathalie; Reekmans, Kristien; Santermans, Eva; Hens, Niel; Goossens, Herman; Verhoye, Marleen; Van der Linden, Annemie; Berneman, Zwi; Hendrix, Sven; Ponsaerts, Peter


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/034302ae-7d44-4a0c-8ba0-11eae1fa8059/12974_2016_Article_756.pdf"
      }, 
      "checksum": "md5:4d7c26d9475244f0057744ba4bbea5c8", 
      "bucket": "034302ae-7d44-4a0c-8ba0-11eae1fa8059", 
      "key": "12974_2016_Article_756.pdf", 
      "type": "pdf", 
      "size": 3512817
    }, 
    {
      "links": {
        "self": "https://zenodo.org/api/files/034302ae-7d44-4a0c-8ba0-11eae1fa8059/12974_2016_Article_756.xml.Meta"
      }, 
      "checksum": "md5:63c9b25e4a4e23f1d4ccf5d2f96f071c", 
      "bucket": "034302ae-7d44-4a0c-8ba0-11eae1fa8059", 
      "key": "12974_2016_Article_756.xml.Meta", 
      "type": "meta", 
      "size": 32424
    }
  ], 
  "owners": [
    22832
  ], 
  "doi": "10.1186/s12974-016-0756-7", 
  "stats": {
    "version_unique_downloads": 24.0, 
    "unique_views": 41.0, 
    "views": 41.0, 
    "version_views": 41.0, 
    "unique_downloads": 24.0, 
    "version_unique_views": 41.0, 
    "volume": 80827215.0, 
    "version_downloads": 24.0, 
    "downloads": 24.0, 
    "version_volume": 80827215.0
  }, 
  "links": {
    "doi": "https://doi.org/10.1186/s12974-016-0756-7", 
    "latest_html": "https://zenodo.org/record/166404", 
    "bucket": "https://zenodo.org/api/files/034302ae-7d44-4a0c-8ba0-11eae1fa8059", 
    "badge": "https://zenodo.org/badge/doi/10.1186/s12974-016-0756-7.svg", 
    "html": "https://zenodo.org/record/166404", 
    "latest": "https://zenodo.org/api/records/166404"
  }, 
  "created": "2016-11-14T03:04:38.687566+00:00", 
  "updated": "2020-01-20T15:47:12.156414+00:00", 
  "conceptrecid": "661963", 
  "revision": 7, 
  "id": 166404, 
  "metadata": {
    "access_right_category": "success", 
    "doi": "10.1186/s12974-016-0756-7", 
    "description": "<p><strong>Background: </strong>Promoting the neuroprotective and repair-inducing effector functions of microglia and macrophages, by means of M2 polarisation or alternative activation, is expected to become a new therapeutic approach for central nervous system (CNS) disorders in which detrimental pro-inflammatory microglia and/or macrophages display a major contribution to the neuropathology. In this study, we present a novel in vivo approach using intracerebral grafting of mesenchymal stem cells (MSC) genetically engineered to secrete interleukin 13 (IL13-MSC).</p><p><strong>Methods: </strong>In the first experimental setup, control MSC and IL13-MSC were grafted in the CNS of eGFP<sup>+</sup> bone marrow chimaeric C57BL/6 mice to histologically evaluate IL13-mediated expression of several markers associated with alternative activation, including arginase1 and Ym1, on MSC graft-recognising microglia and MSC graft-infiltrating macrophages. In the second experimental setup, IL13-MSC were grafted on the right side (or on both the right and left sides) of the <i>splenium</i> of the <i>corpus callosum</i> in wild-type C57BL/6 mice and in C57BL/6 CX<sub>3</sub>CR1<sup>eGFP/+</sup>CCR2<sup>RFP/+</sup> transgenic mice. Next, CNS inflammation and demyelination was induced by means of a cuprizone-supplemented diet. The influence of IL13-MSC grafting on neuropathological alterations was monitored by non-invasive <i>T</i> <sub>2</sub>-weighted magnetic resonance imaging (MRI) and quantitative histological analyses, as compared to cuprizone-treated mice with control MSC grafts and/or cuprizone-treated mice without MSC injection.</p><p><strong>Results: </strong>In the first part of this study, we demonstrate that MSC graft-associated microglia and MSC graft-infiltrating macrophages are forced into alternative activation upon grafting of IL13-MSC, but not upon grafting of control MSC. In the second part of this study, we demonstrate that grafting of IL13-MSC, in addition to the recruitment of M2 polarised macrophages, limits cuprizone-induced microgliosis, oligodendrocyte death and demyelination. Furthermore, we here demonstrate that injection of IL13-MSC at both sides of the splenium leads to a superior protective effect as compared to a single injection at one side of the splenium.</p><p><strong>Conclusions: </strong>Controlled and localised production of IL13 by means of intracerebral MSC grafting has the potential to modulate cell graft- and pathology-associated microglial/macrophage responses, and to interfere with oligodendrocyte death and demyelinating events in the cuprizone mouse model.</p>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model", 
    "journal": {
      "volume": "13", 
      "issue": "1", 
      "pages": "288", 
      "title": "Journal of Neuroinflammation"
    }, 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "661963"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "166404"
          }
        }
      ]
    }, 
    "communities": [
      {
        "id": "ecfunded"
      }, 
      {
        "id": "fp7-bmc"
      }
    ], 
    "grants": [
      {
        "code": "278850", 
        "links": {
          "self": "https://zenodo.org/api/grants/10.13039/501100000780::278850"
        }, 
        "title": "Imaging of Neuroinflammation in Neurodegenerative Diseases", 
        "acronym": "INMIND", 
        "program": "FP7", 
        "funder": {
          "doi": "10.13039/501100000780", 
          "acronyms": [], 
          "name": "European Commission", 
          "links": {
            "self": "https://zenodo.org/api/funders/10.13039/501100000780"
          }
        }
      }
    ], 
    "keywords": [
      "Interleukin 13", 
      "Mesenchymal stem cells", 
      "Neuroinflammation", 
      "Transplantation", 
      "Magnetic resonance imaging"
    ], 
    "publication_date": "2016-11-09", 
    "creators": [
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Le Blon, Debbie"
      }, 
      {
        "affiliation": "Bio-Imaging Laboratory, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Guglielmetti, Caroline"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Hoornaert, Chlo\u00e9"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Quarta, Alessandra"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Daans, Jasmijn"
      }, 
      {
        "affiliation": "Department of Morphology, Biomedical Research Institute, Hasselt University, Agoralaan building C, 3590, Diepenbeek, Belgium", 
        "name": "Dooley, Dearbhaile"
      }, 
      {
        "affiliation": "Department of Morphology, Biomedical Research Institute, Hasselt University, Agoralaan building C, 3590, Diepenbeek, Belgium", 
        "name": "Lemmens, Evi"
      }, 
      {
        "affiliation": "Bio-Imaging Laboratory, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Praet, Jelle"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "De Vocht, Nathalie"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Reekmans, Kristien"
      }, 
      {
        "affiliation": "Center for Statistics, I-Biostat, Hasselt University, Agoralaan building D, 3590, Diepenbeek, Belgium", 
        "name": "Santermans, Eva"
      }, 
      {
        "affiliation": "Center for Statistics, I-Biostat, Hasselt University, Agoralaan building D, 3590, Diepenbeek, Belgium", 
        "name": "Hens, Niel"
      }, 
      {
        "affiliation": "Vaccine and Infectious Disease Institute, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Goossens, Herman"
      }, 
      {
        "affiliation": "Bio-Imaging Laboratory, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Verhoye, Marleen"
      }, 
      {
        "affiliation": "Bio-Imaging Laboratory, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Van der Linden, Annemie"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Berneman, Zwi"
      }, 
      {
        "affiliation": "Department of Morphology, Biomedical Research Institute, Hasselt University, Agoralaan building C, 3590, Diepenbeek, Belgium", 
        "name": "Hendrix, Sven"
      }, 
      {
        "affiliation": "Laboratory of Experimental Hematology, University of Antwerp, Universiteitsplein 1, 2610, Antwerp, Belgium", 
        "name": "Ponsaerts, Peter"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "article", 
      "type": "publication", 
      "title": "Journal article"
    }
  }
}
41
24
views
downloads
Views 41
Downloads 24
Data volume 80.8 MB
Unique views 41
Unique downloads 24

Share

Cite as