Journal article Open Access

[18F]GE-180: a novel TSPO radiotracer compared to [11C]PK11195 in a preclinical model of stroke.

Boutin, Hervé; Murray, Katie; Pradillo, Jesus; Maroy, Renaud; Smigova, Alison; Gerhard, Alexander; Jones, Paul A; Trigg, William


JSON Export

{
  "files": [
    {
      "links": {
        "self": "https://zenodo.org/api/files/3f415b08-86e4-4906-92b6-00a59f22ca26/Boutin_EurJNuclMedMolImaging_2015-P10b-AAM.pdf"
      }, 
      "checksum": "md5:94ecc8978809b48a79171daab7dc3a88", 
      "bucket": "3f415b08-86e4-4906-92b6-00a59f22ca26", 
      "key": "Boutin_EurJNuclMedMolImaging_2015-P10b-AAM.pdf", 
      "type": "pdf", 
      "size": 1754067
    }
  ], 
  "owners": [
    1964
  ], 
  "doi": "10.1007/s00259-014-2939-8", 
  "stats": {
    "version_unique_downloads": 20.0, 
    "unique_views": 19.0, 
    "views": 20.0, 
    "downloads": 20.0, 
    "unique_downloads": 20.0, 
    "version_unique_views": 19.0, 
    "volume": 35081340.0, 
    "version_downloads": 20.0, 
    "version_views": 20.0, 
    "version_volume": 35081340.0
  }, 
  "links": {
    "doi": "https://doi.org/10.1007/s00259-014-2939-8", 
    "latest_html": "https://zenodo.org/record/15873", 
    "bucket": "https://zenodo.org/api/files/3f415b08-86e4-4906-92b6-00a59f22ca26", 
    "badge": "https://zenodo.org/badge/doi/10.1007/s00259-014-2939-8.svg", 
    "html": "https://zenodo.org/record/15873", 
    "latest": "https://zenodo.org/api/records/15873"
  }, 
  "created": "2015-03-13T15:45:32+00:00", 
  "updated": "2019-04-10T03:39:55.881700+00:00", 
  "conceptrecid": "608561", 
  "revision": 10, 
  "id": 15873, 
  "metadata": {
    "access_right_category": "success", 
    "embargo_date": "2015-10-29", 
    "doi": "10.1007/s00259-014-2939-8", 
    "description": "<p>PURPOSE: Neuroinflammation plays a critical role in various neuropathological conditions, and hence there is renewed interest in the translocator protein (TSPO) as a biomarker of microglial activation and macrophage infiltration in the brain. This is reflected in the large amount of research conducted seeking to replace the prototypical PET radiotracer 11C-R-PK11195 with a TSPO ligand with higher performance. Here we report the in vivo preclinical investigation of the novel TSPO tracer 18F-GE-180 in a rat model of stroke.</p>\n\n<p>METHODS: Focal cerebral ischaemia was induced in Wistar rats by 60-min occlusion of the middle cerebral artery (MCAO). Brain damage was assessed 24 h after MCAO by T2 MRI. Rats were scanned with 11C-R-PK11195 and 18F-GE-180 5 or 6 days after MCAO. Specificity of binding was confirmed by injection of unlabelled R-PK11195 or GE-180 20 min after injection of 18F-GE-180. In vivo data were confirmed by ex vivo immunohistochemistry for microglial (CD11b) and astrocytic biomarkers (GFAP).</p>\n\n<p>RESULTS: 18F-GE-180 uptake was 24 % higher in the core of the ischaemic lesion and 18 % lower in the contralateral healthy tissue than that of 11C-R-PK11195 uptake (1.5&thinsp;&plusmn;&thinsp;0.2-fold higher signal to noise ratio). We confirmed this finding using the simplified reference tissue model (BPND&thinsp;=&thinsp;3.5&thinsp;&plusmn;&thinsp;0.4 and 2.4&thinsp;&plusmn;&thinsp;0.5 for 18F-GE-180 and 11C-R-PK11195, respectively, with R 1 = 1). Injection of unlabelled R-PK11195 or GE-180 20 min after injection of 18F-GE-180 significantly displaced 18F-GE-180 (69&thinsp;&plusmn;&thinsp;5 % and 63&thinsp;&plusmn;&thinsp;4 %, respectively). Specificity of the binding was also confirmed by in vitro autoradiography, and the location and presence of activated microglia and infiltrated macrophages were confirmed by immunohistochemistry.</p>\n\n<p>CONCLUSION: The in vivo binding characteristics of 18F-GE-180 demonstrate a better signal to noise ratio than 11C-R-PK11195 due to both a better signal in the lesion and lower nonspecific binding in healthy tissue. These results provide evidence that 18F-GE-180 is a strong candidate to replace 11C-R-PK11195.</p>", 
    "license": {
      "id": "CC-BY-4.0"
    }, 
    "title": "[18F]GE-180: a novel TSPO radiotracer compared to [11C]PK11195 in a preclinical model of stroke.", 
    "journal": {
      "volume": "42", 
      "year": "2014", 
      "issue": "3", 
      "pages": "503-11", 
      "title": "Eur J Nucl Med Mol Imaging"
    }, 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "608561"
          }, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "15873"
          }
        }
      ]
    }, 
    "communities": [
      {
        "id": "inmind"
      }, 
      {
        "id": "ecfunded"
      }
    ], 
    "grants": [
      {
        "code": "278850", 
        "links": {
          "self": "https://zenodo.org/api/grants/10.13039/501100000780::278850"
        }, 
        "title": "Imaging of Neuroinflammation in Neurodegenerative Diseases", 
        "acronym": "INMIND", 
        "program": "FP7", 
        "funder": {
          "doi": "10.13039/501100000780", 
          "acronyms": [
            "EC"
          ], 
          "name": "European Commission", 
          "links": {
            "self": "https://zenodo.org/api/funders/10.13039/501100000780"
          }
        }
      }
    ], 
    "keywords": [
      "positron emission tomography", 
      "translocator protein", 
      " R-PK11195", 
      "brain ischemia", 
      "GE-180"
    ], 
    "publication_date": "2014-10-29", 
    "creators": [
      {
        "affiliation": "Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK and Wolfson Molecular Imaging Centre, University of Manchester, Manchester, UK", 
        "name": "Boutin, Herv\u00e9"
      }, 
      {
        "affiliation": "Faculty of Life Sciences, University of Manchester, Manchester, UK", 
        "name": "Murray, Katie"
      }, 
      {
        "affiliation": "Faculty of Life Sciences, University of Manchester, Manchester, UK; Present address: Universidad Complutense/Politecnica de Madrid, Madrid, Spain", 
        "name": "Pradillo, Jesus"
      }, 
      {
        "affiliation": "SHFJ - CEA Orsay, France", 
        "name": "Maroy, Renaud"
      }, 
      {
        "affiliation": "Wolfson Molecular Imaging Centre, University of Manchester, Manchester, UK", 
        "name": "Smigova, Alison"
      }, 
      {
        "affiliation": "Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK and Wolfson Molecular Imaging Centre, University of Manchester, Manchester, UK", 
        "name": "Gerhard, Alexander"
      }, 
      {
        "affiliation": "GE Healthcare Ltd., Amersham, UK", 
        "name": "Jones, Paul A"
      }, 
      {
        "affiliation": "GE Healthcare Ltd., Amersham, UK", 
        "name": "Trigg, William"
      }
    ], 
    "access_right": "open", 
    "resource_type": {
      "subtype": "article", 
      "type": "publication", 
      "title": "Journal article"
    }
  }
}
20
20
views
downloads
Views 20
Downloads 20
Data volume 35.1 MB
Unique views 19
Unique downloads 20

Share

Cite as