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Published September 28, 2016 | Version v1
Poster Open

Biosynthesis of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) and Investigation of Their Cytotoxicity Effects on Human Mesenchymal Stem Cells (hMSCs)

  • 1. Mashhad University of Medical Sciences

Description

Superparamagnetic iron oxide nanoparticles (SPIONs; Fe3O4) are one of the most versatile and safe nanoparticles in a wide variety of biomedical applications, for example, magnetic resonance imaging (MRI), targeted delivery of drugs or genes, and in hyperthermia. Although, the potential benefits of SPIONs are considerable, there is a distinct need to identify any potential cellular damage associated with these nanoparticles. In this work, the SPIONs were synthesized by the covalent binding of starch onto the surface of SPIONs, by a ?green? co?precipitation method. The field emission scanning electron microscopy (FESEM) illustrated the regular spheres of SPIONs that were coated by starch molecules with a mean diameter of about 30 nm. The X?ray diffraction (XRD) patterns indicated that the biosynthesized samples were pure Fe3O4 with a spinel structure while the coating of starch did not result in a phase change. The starch coating onto the SPIONs was also demonstrated by Fourier transform infrared (FTIR) spectra and magnetic measurements revealed that the saturated magnetization of the starch ? SPIONs reached 38.4 emu/g, having nanoparticles with the characteristics of superparamagnetism. In vitro cytotoxicity studies on human mesenchymal stem cells (hMSCs) showed a dose dependent toxicity with non-toxic effect of concentration below 31.25 ?g/mL. In this study, we have focused on biosynthesis of SPIONs and their induced toxic biological responses for a better toxicological understanding of SPIONs.

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