{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSPJ9hGurOzZIXe","_score":1,"_source":{"id":["14711381"],"source":["MED"],"pmid":["14711381"],"pmcid":["PMC320490"],"doi":["10.1186/1475-2832-3-1"],"title":["Behavioral and antioxidant activity of a tosylbenz[g]indolamine derivative. A proposed better profile for a potential antipsychotic agent."],"authorString":["Zika CA, Nicolaou I, Gavalas A, Rekatas GV, Tani E, Demopoulos VJ."],"authorList":[{"author":[{"fullName":["Zika CA"],"firstName":["Chara A"],"lastName":["Zika"],"initials":["CA"],"affiliation":["Department of Pharmaceutical Chemistry, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, 54124 Greece. chzika@pharm.auth.gr"]},{"fullName":["Nicolaou I"],"firstName":["Ioannis"],"lastName":["Nicolaou"],"initials":["I"]},{"fullName":["Gavalas A"],"firstName":["Antonis"],"lastName":["Gavalas"],"initials":["A"]},{"fullName":["Rekatas GV"],"firstName":["George V"],"lastName":["Rekatas"],"initials":["GV"]},{"fullName":["Tani E"],"firstName":["Ekaterini"],"lastName":["Tani"],"initials":["E"]},{"fullName":["Demopoulos VJ"],"firstName":["Vassilis J"],"lastName":["Demopoulos"],"initials":["VJ"]}]}],"journalInfo":[{"issue":["1"],"volume":["3"],"journalIssueId":["1243149"],"dateOfPublication":["2004 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2004"],"printPublicationDate":["2004-01-01"],"journal":[{"title":["Annals of general hospital psychiatry"],"ISOAbbreviation":["Ann Gen Hosp Psychiatry"],"medlineAbbreviation":["Ann Gen Hosp Psychiatry"],"NLMid":["101147431"],"ESSN":["1475-2832"]}]}],"pubYear":["2004"],"pageInfo":["1"],"abstractText":["BACKGROUND: Tardive dyskinesia (TD) is a major limitation of older antipsychotics. Newer antipsychotics have various other side effects such as weight gain, hyperglycemia, etc. In a previous study we have shown that an indolamine molecule expresses a moderate binding affinity at the dopamine D2 and serotonin 5-HT1A receptors in in vitro competition binding assays. In the present work, we tested its p-toluenesulfonyl derivative (TPBIA) for behavioral effects in rats, related to interactions with central dopamine receptors and its antioxidant activity. METHODS: Adult male Fischer-344 rats grouped as: i) Untreated rats: TPBIA was administered i.p. in various doses ii) Apomorphine-treated rats: were treated with apomorphine (1 mg kg-1, i.p.) 10 min after the administration of TPBIA. Afterwards the rats were placed individually in the activity cage and their motor behaviour was recorded for the next 30 min The antioxidant potential of TPBIA was investigated in the model of in vitro non enzymatic lipid peroxidation. RESULTS: i) In non-pretreated rats, TPBIA reduces the activity by 39 and 82% respectively, ii) In apomorphine pretreated rats, TPBIA reverses the hyperactivity and stereotype behaviour induced by apomorphine. Also TPBIA completely inhibits the peroxidation of rat liver microsome preparations at concentrations of 0.5, 0.25 and 0.1 mM. CONCLUSION: TPBIA exerts dopamine antagonistic activity in the central nervous system. In addition, its antioxidant effect is a desirable property, since TD has been partially attributed, to oxidative stress. Further research is needed to test whether TPBIA may be used as an antipsychotic agent."],"affiliation":["Department of Pharmaceutical Chemistry, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, 54124 Greece. chzika@pharm.auth.gr"],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC320490?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC320490"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1475-2832-3-1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["5"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2004-01-27"],"electronicPublicationDate":["2004-01-07"],"firstPublicationDate":["2004-01-01"],"cprojectID":"PMC320490"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSWJ9hGurOzZIXo","_score":1,"_source":{"id":["23413435"],"source":["MED"],"pmid":["23413435"],"pmcid":["PMC3605601"],"doi":["10.1093/bioinformatics/btt066"],"title":["A new way to protect privacy in large-scale genome-wide association studies."],"authorString":["Kamm L, Bogdanov D, Laur S, Vilo J."],"authorList":[{"author":[{"fullName":["Kamm L"],"firstName":["Liina"],"lastName":["Kamm"],"initials":["L"]},{"fullName":["Bogdanov D"],"firstName":["Dan"],"lastName":["Bogdanov"],"initials":["D"],"authorId":[{"_":"0000-0002-9296-9120","$":{"type":"ORCID"}}]},{"fullName":["Laur S"],"firstName":["Sven"],"lastName":["Laur"],"initials":["S"]},{"fullName":["Vilo J"],"firstName":["Jaak"],"lastName":["Vilo"],"initials":["J"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9296-9120","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["29"],"journalIssueId":["2022931"],"dateOfPublication":["2013 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2013"],"printPublicationDate":["2013-04-01"],"journal":[{"title":["Bioinformatics (Oxford, England)"],"ISOAbbreviation":["Bioinformatics"],"medlineAbbreviation":["Bioinformatics"],"NLMid":["9808944"],"ISSN":["1367-4803"],"ESSN":["1367-4811"]}]}],"pubYear":["2013"],"pageInfo":["886-893"],"abstractText":[" MOTIVATION: Increased availability of various genotyping techniques has initiated a race for finding genetic markers that can be used in diagnostics and personalized medicine. Although many genetic risk factors are known, key causes of common diseases with complex heritage patterns are still unknown. Identification of such complex traits requires a targeted study over a large collection of data. Ideally, such studies bring together data from many biobanks. However, data aggregation on such a large scale raises many privacy issues. RESULTS: We show how to conduct such studies without violating privacy of individual donors and without leaking the data to third parties. The presented solution has provable security guarantees. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online."],"affiliation":["Institute of Computer Science, University of Tartu, Liivi 2, Tartu 50409, Estonia."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Data Interpretation, Statistical"]},{"majorTopic_YN":["N"],"descriptorName":["Case-Control Studies"]},{"majorTopic_YN":["Y"],"descriptorName":["Privacy"]},{"majorTopic_YN":["Y"],"descriptorName":["Genome-Wide Association Study"]},{"majorTopic_YN":["N"],"descriptorName":["Genotyping Techniques"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3605601?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3605601"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23413435"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23413435&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/bioinformatics/btt066"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["8"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-09-20"],"dateOfCreation":["2013-03-25"],"electronicPublicationDate":["2013-02-14"],"firstPublicationDate":["2013-02-14"],"cprojectID":"PMC3605601"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSVhOUS2orL1INx","_score":1,"_source":{"id":["9621201"],"source":["MED"],"pmid":["9621201"],"pmcid":["PMC2640145"],"doi":["10.3201/eid0402.980218"],"title":["New vectors of Rift Valley fever in West Africa."],"authorString":["Fontenille D, Traore-Lamizana M, Diallo M, Thonnon J, Digoutte JP, Zeller HG."],"authorList":[{"author":[{"fullName":["Fontenille D"],"lastName":["Fontenille"],"initials":["D"]},{"fullName":["Traore-Lamizana M"],"lastName":["Traore-Lamizana"],"initials":["M"]},{"fullName":["Diallo M"],"lastName":["Diallo"],"initials":["M"]},{"fullName":["Thonnon J"],"lastName":["Thonnon"],"initials":["J"]},{"fullName":["Digoutte JP"],"lastName":["Digoutte"],"initials":["JP"]},{"fullName":["Zeller HG"],"lastName":["Zeller"],"initials":["HG"]}]}],"journalInfo":[{"issue":["2"],"volume":["4"],"journalIssueId":["909524"],"dateOfPublication":["1998 Apr-Jun"],"monthOfPublication":["0"],"yearOfPublication":["1998"],"printPublicationDate":["1998-04-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["1998"],"pageInfo":["289-293"],"abstractText":["After an outbreak of Rift Valley fever in Southern Mauritania in 1987, entomologic studies were conducted in a bordering region in Sénégal from 1991 to 1996 to identify the sylvatic vectors of Rift Valley fever virus. The virus was isolated from the floodwater mosquitoes Aedes vexans and Ae. ochraceus. In 1974 and 1983, the virus had been isolated from Ae. dalzieli. Although these vectors differ from the main vectors in East and South Africa, they use the same type of breeding sites and also feed on cattle and sheep. Although enzootic vectors have now been identified in West Africa, the factors causing outbreaks remain unclear."],"affiliation":["Laboratoire de zoologie médicale, Institut Pasteur, Dakar, Sénégal. fontenil@dakar.orstom.sn"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Cattle"]},{"majorTopic_YN":["N"],"descriptorName":["Sheep"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Culex"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Psychodidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Rift Valley fever virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Rift Valley Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Seasons"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Africa, Western"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2640145?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2640145"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid0402.980218"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["74"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["1998-09-01"],"dateOfCreation":["1998-09-01"],"dateOfRevision":["2009-11-18"],"firstPublicationDate":["1998-04-01"],"cprojectID":"PMC2640145"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSahOUS2orL1IN0","_score":1,"_source":{"id":["21695153"],"source":["MED"],"pmid":["21695153"],"pmcid":["PMC3113902"],"doi":["10.1371/journal.pone.0021045"],"title":["Efficient immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases."],"authorString":["Flisikowska T, Thorey IS, Offner S, Ros F, Lifke V, Zeitler B, Rottmann O, Vincent A, Zhang L, Jenkins S, Niersbach H, Kind AJ, Gregory PD, Schnieke AE, Platzer J."],"authorList":[{"author":[{"fullName":["Flisikowska T"],"firstName":["Tatiana"],"lastName":["Flisikowska"],"initials":["T"]},{"fullName":["Thorey IS"],"firstName":["Irmgard S"],"lastName":["Thorey"],"initials":["IS"]},{"fullName":["Offner S"],"firstName":["Sonja"],"lastName":["Offner"],"initials":["S"]},{"fullName":["Ros F"],"firstName":["Francesca"],"lastName":["Ros"],"initials":["F"]},{"fullName":["Lifke V"],"firstName":["Valeria"],"lastName":["Lifke"],"initials":["V"]},{"fullName":["Zeitler B"],"firstName":["Bryan"],"lastName":["Zeitler"],"initials":["B"]},{"fullName":["Rottmann O"],"firstName":["Oswald"],"lastName":["Rottmann"],"initials":["O"]},{"fullName":["Vincent A"],"firstName":["Anna"],"lastName":["Vincent"],"initials":["A"]},{"fullName":["Zhang L"],"firstName":["Lei"],"lastName":["Zhang"],"initials":["L"]},{"fullName":["Jenkins S"],"firstName":["Shirin"],"lastName":["Jenkins"],"initials":["S"]},{"fullName":["Niersbach H"],"firstName":["Helmut"],"lastName":["Niersbach"],"initials":["H"]},{"fullName":["Kind AJ"],"firstName":["Alexander J"],"lastName":["Kind"],"initials":["AJ"]},{"fullName":["Gregory PD"],"firstName":["Philip D"],"lastName":["Gregory"],"initials":["PD"]},{"fullName":["Schnieke AE"],"firstName":["Angelika E"],"lastName":["Schnieke"],"initials":["AE"]},{"fullName":["Platzer J"],"firstName":["Josef"],"lastName":["Platzer"],"initials":["J"]}]}],"journalInfo":[{"issue":["6"],"volume":["6"],"journalIssueId":["1839354"],"dateOfPublication":["2011 "],"monthOfPublication":["0"],"yearOfPublication":["2011"],"printPublicationDate":["2011-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2011"],"pageInfo":["e21045"],"abstractText":["Rabbits are widely used in biomedical research, yet techniques for their precise genetic modification are lacking. We demonstrate that zinc finger nucleases (ZFNs) introduced into fertilized oocytes can inactivate a chosen gene by mutagenesis and also mediate precise homologous recombination with a DNA gene-targeting vector to achieve the first gene knockout and targeted sequence replacement in rabbits. Two ZFN pairs were designed that target the rabbit immunoglobulin M (IgM) locus within exons 1 and 2. ZFN mRNAs were microinjected into pronuclear stage fertilized oocytes. Founder animals carrying distinct mutated IgM alleles were identified and bred to produce offspring. Functional knockout of the immunoglobulin heavy chain locus was confirmed by serum IgM and IgG deficiency and lack of IgM(+) and IgG(+) B lymphocytes. We then tested whether ZFN expression would enable efficient targeted sequence replacement in rabbit oocytes. ZFN mRNA was co-injected with a linear DNA vector designed to replace exon 1 of the IgM locus with ∼1.9 kb of novel sequence. Double strand break induced targeted replacement occurred in up to 17% of embryos and in 18% of fetuses analyzed. Two major goals have been achieved. First, inactivation of the endogenous IgM locus, which is an essential step for the production of therapeutic human polyclonal antibodies in the rabbit. Second, establishing efficient targeted gene manipulation and homologous recombination in a refractory animal species. ZFN mediated genetic engineering in the rabbit and other mammals opens new avenues of experimentation in immunology and many other research fields."],"affiliation":["Chair of Livestock Biotechnology, Technische Universität München, Freising, Germany."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Oocytes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Rabbits"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Deoxyribonucleases"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin G"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin M"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DF"],"qualifierName":["deficiency"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Messenger"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Microinjections"]},{"majorTopic_YN":["N"],"descriptorName":["Reproducibility of Results"]},{"majorTopic_YN":["N"],"descriptorName":["Protein Engineering"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Base Sequence"]},{"majorTopic_YN":["Y"],"descriptorName":["Zinc Fingers"]},{"majorTopic_YN":["N"],"descriptorName":["Mutation"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Alleles"]},{"majorTopic_YN":["N"],"descriptorName":["Exons"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Gene Knockout Techniques"]},{"majorTopic_YN":["N"],"descriptorName":["Genetic Loci"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Messenger"],"registryNumber":["0"]},{"name":["Immunoglobulin G"],"registryNumber":["0"]},{"name":["Immunoglobulin M"],"registryNumber":["0"]},{"name":["Deoxyribonucleases"],"registryNumber":["EC 3.1.-"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3113902?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3113902"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=21695153"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=21695153&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0021045"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["52"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2011-10-21"],"dateOfCreation":["2011-06-22"],"dateOfRevision":["2013-06-28"],"electronicPublicationDate":["2011-06-13"],"firstPublicationDate":["2011-06-13"],"cprojectID":"PMC3113902"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqShhOUS2orL1IOD","_score":1,"_source":{"id":["22461730"],"source":["MED"],"pmid":["22461730"],"pmcid":["PMC3310194"],"doi":["10.3897/phytokeys.10.2734"],"title":["Taxonomic synopsis of invasive and native Spartina (Poaceae, Chloridoideae) in the Pacific Northwest (British Columbia, Washington and Oregon), including the first report of Spartina ×townsendii for British Columbia, Canada."],"authorString":["Saarela JM."],"authorList":[{"author":[{"fullName":["Saarela JM"],"firstName":["Jeffery M"],"lastName":["Saarela"],"initials":["JM"]}]}],"journalInfo":[{"issue":["10"],"journalIssueId":["1918436"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["PhytoKeys"],"ISOAbbreviation":["PhytoKeys"],"medlineAbbreviation":["PhytoKeys"],"NLMid":["101548554"],"ISSN":["1314-2011"],"ESSN":["1314-2003"]}]}],"pubYear":["2012"],"pageInfo":["25-82"],"abstractText":["Five species of the grass genus Spartina are invading salt marshes along the Pacific coast of North America, of which three have been documented in British Columbia, Canada, in only the last decade. A taxonomic synopsis of the two native (Spartina gracilis, Spartina pectinata) and five introduced Spartina taxa (Spartina anglica, Spartina alterniflora, Spartina densiflora, Spartina patens, Spartina ×townsendii) in the Pacific Northwest is presented to facilitate their identification, including nomenclature, a new taxonomic key, new descriptions for a subset of taxa, and representative specimens. Spartina ×townsendii is newly reported for the flora of British Columbia. The non-coastal species Spartina pectinata is reported from an urban site in British Columbia, the first confirmed report of the taxon for the province. Lectotypes are newly designated for Spartina anglica C.E. Hubb., Spartina maritima subvar. fallax St.-Yves, and Spartina cynosuroides f. major St.-Yves."],"affiliation":["Research & Collections, Canadian Museum of Nature, P.O. Box 3443 Stn. D, Ottawa, Ontario K1P 6P4, Canada."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Species identification","Spartina","Invasive Grasses","Voucher Specimens","Coastal Habitats","Grass Taxonomy"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3310194?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3310194"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22461730"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22461730&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3897/phytokeys.10.2734"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2012-08-23"],"dateOfCreation":["2012-03-30"],"dateOfRevision":["2013-05-29"],"electronicPublicationDate":["2012-03-21"],"firstPublicationDate":["2012-03-21"],"cprojectID":"PMC3310194"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqST_8VqgcuJwvfp","_score":1,"_source":{"id":["22389730"],"source":["MED"],"pmid":["22389730"],"pmcid":["PMC3289602"],"doi":["10.1371/journal.pntd.0001477"],"title":["Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage."],"authorString":["Haddow AD, Schuh AJ, Yasuda CY, Kasper MR, Heang V, Huy R, Guzman H, Tesh RB, Weaver SC."],"authorList":[{"author":[{"fullName":["Haddow AD"],"firstName":["Andrew D"],"lastName":["Haddow"],"initials":["AD"],"affiliation":["Institute for Human Infections and Immunity, Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA. adhaddow@gmail.com"]},{"fullName":["Schuh AJ"],"firstName":["Amy J"],"lastName":["Schuh"],"initials":["AJ"]},{"fullName":["Yasuda CY"],"firstName":["Chadwick Y"],"lastName":["Yasuda"],"initials":["CY"]},{"fullName":["Kasper MR"],"firstName":["Matthew R"],"lastName":["Kasper"],"initials":["MR"]},{"fullName":["Heang V"],"firstName":["Vireak"],"lastName":["Heang"],"initials":["V"]},{"fullName":["Huy R"],"firstName":["Rekol"],"lastName":["Huy"],"initials":["R"]},{"fullName":["Guzman H"],"firstName":["Hilda"],"lastName":["Guzman"],"initials":["H"]},{"fullName":["Tesh RB"],"firstName":["Robert B"],"lastName":["Tesh"],"initials":["RB"]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8957-2608","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["6"],"journalIssueId":["1942889"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2012"],"pageInfo":["e1477"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne flavivirus distributed throughout much of Africa and Asia. Infection with the virus may cause acute febrile illness that clinically resembles dengue fever. A recent study indicated the existence of three geographically distinct viral lineages; however this analysis utilized only a single viral gene. Although ZIKV has been known to circulate in both Africa and Asia since at least the 1950s, little is known about the genetic relationships between geographically distinct virus strains. Moreover, the geographic origin of the strains responsible for the epidemic that occurred on Yap Island, Federated States of Micronesia in 2007, and a 2010 pediatric case in Cambodia, has not been determined.To elucidate the genetic relationships of geographically distinct ZIKV strains and the origin of the strains responsible for the 2007 outbreak on Yap Island and a 2010 Cambodian pediatric case of ZIKV infection, the nucleotide sequences of the open reading frame of five isolates from Cambodia, Malaysia, Nigeria, Uganda, and Senegal collected between 1947 and 2010 were determined. Phylogenetic analyses of these and previously published ZIKV sequences revealed the existence of two main virus lineages (African and Asian) and that the strain responsible for the Yap epidemic and the Cambodian case most likely originated in Southeast Asia. Examination of the nucleotide and amino acid sequence alignments revealed the loss of a potential glycosylation site in some of the virus strains, which may correlate with the passage history of the virus.The basal position of the ZIKV strain isolated in Malaysia in 1966 suggests that the recent outbreak in Micronesia was initiated by a strain from Southeast Asia. Because ZIKV infection in humans produces an illness clinically similar to dengue fever and many other tropical infectious diseases, it is likely greatly misdiagnosed and underreported."],"affiliation":["Institute for Human Infections and Immunity, Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA. adhaddow@gmail.com"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S.","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["AI069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["HHSN27200004/D04"],"agency":["PHS HHS"],"orderIn":["0"]},{"grantId":["T-32 AI60549"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["T-32 A1007536"],"agency":["PHS HHS"],"orderIn":["0"]},{"grantId":["HHSN272201000040I"],"agency":["PHS HHS"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["N"],"descriptorName":["Genotype"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Africa"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Asia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Micronesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Epidemiology"]},{"majorTopic_YN":["Y"],"descriptorName":["Phylogeography"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3289602?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3289602"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22389730&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22389730"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0001477"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["33"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2012-06-22"],"dateOfCreation":["2012-03-05"],"dateOfRevision":["2016-02-05"],"electronicPublicationDate":["2012-02-28"],"firstPublicationDate":["2012-02-28"],"cprojectID":"PMC3289602"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqUg_8VqgcuJwvf5","_score":1,"_source":{"id":["23410000"],"source":["MED"],"pmid":["23410000"],"pmcid":["PMC3616844"],"doi":["10.1186/1743-422x-10-58"],"title":["Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses."],"authorString":["Patel P, Landt O, Kaiser M, Faye O, Koppe T, Lass U, Sall AA, Niedrig M."],"authorList":[{"author":[{"fullName":["Patel P"],"firstName":["Pranav"],"lastName":["Patel"],"initials":["P"]},{"fullName":["Landt O"],"firstName":["Olfert"],"lastName":["Landt"],"initials":["O"]},{"fullName":["Kaiser M"],"firstName":["Marco"],"lastName":["Kaiser"],"initials":["M"]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"]},{"fullName":["Koppe T"],"firstName":["Tanja"],"lastName":["Koppe"],"initials":["T"]},{"fullName":["Lass U"],"firstName":["Ulrich"],"lastName":["Lass"],"initials":["U"]},{"fullName":["Sall AA"],"firstName":["Amadou A"],"lastName":["Sall"],"initials":["AA"]},{"fullName":["Niedrig M"],"firstName":["Matthias"],"lastName":["Niedrig"],"initials":["M"]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2002717"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2013"],"pageInfo":["58"],"abstractText":[" BACKGROUND: The genus Flavivirus includes several pathogenic agents that cause severe illness in humans. Re-emergence of West Nile virus in Europe and continuous spread of certain flaviviruses such as dengue, yellow fever and Japanese encephalitis viruses represent a global danger to public health. Therefore, a rapid and accurate molecular method is required for diagnostics and epidemiological surveillance of flaviviruses. METHODS: A Pan-Flavi quantitative RT-PCR assay using a Locked-Nucleic Acid probe targeting the flavivirus NS5 gene was developed and optimized to detect a wide range of flaviviruses simultaneously. The specificity and sensitivity of the Pan-Flavi assay were tested using RNA of different flaviviruses and non-flaviviruses. Furthermore, the assay was compared directly to flavivirus species-specific assays for the ability to detect flaviviruses sensitively. RESULTS: Two degenerate primers and one Locked-Nucleic Acids probe were designed to amplify most of the flaviviruses. To increase the specificity and fluorescence signal of the Pan-Flavi assay for detection of yellow fever virus and dengue virus 4, additional primers and probes were included. Viral RNA of thirty different flaviviruses was detected, verifying the broad range specificity. The testing of this assay was successful, using standard plasmid and RNA dilutions of yellow fever virus vaccine strain, dengue virus 1 and tick-borne encephalitis virus, with a sensitivity limit of 10-100 genome copies/reaction. Also comparatively good results were achieved for detecting different flaviviruses by the Pan-Flavi assay when compared to the flavivirus species-specific assays. CONCLUSION: The assay is rapid, broad-range flavivirus-specific and highly sensitive making it a valuable tool for rapid detection of flaviviruses in livestock samples, epidemiological studies or as useful complement to single flavivirus-specific assays for clinical diagnosis."],"affiliation":["Robert Koch Institute, Center for Biological Security 1 (ZBS1), Nordufer 20, Berlin, 13353, Germany. patelp@rki.de"],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA Primers"]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Species Specificity"]}]}],"chemicalList":[{"chemical":[{"name":["DNA Primers"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3616844?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3616844"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23410000"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23410000&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1743-422X-10-58"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["8"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-09-12"],"dateOfCreation":["2013-04-05"],"electronicPublicationDate":["2013-02-14"],"firstPublicationDate":["2013-02-14"],"cprojectID":"PMC3616844"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSW_8VqgcuJwvfx","_score":1,"_source":{"id":["20740316"],"source":["MED"],"pmid":["20740316"],"pmcid":["PMC3023858"],"doi":["10.1007/s11136-010-9732-3"],"title":["Current quality of life and its determinants among opiate-dependent individuals five years after starting methadone treatment."],"authorString":["De Maeyer J, Vanderplasschen W, Lammertyn J, van Nieuwenhuizen C, Sabbe B, Broekaert E."],"authorList":[{"author":[{"fullName":["De Maeyer J"],"firstName":["Jessica"],"lastName":["De Maeyer"],"initials":["J"]},{"fullName":["Vanderplasschen W"],"firstName":["Wouter"],"lastName":["Vanderplasschen"],"initials":["W"]},{"fullName":["Lammertyn J"],"firstName":["Jan"],"lastName":["Lammertyn"],"initials":["J"],"authorId":[{"_":"0000-0002-1263-1059","$":{"type":"ORCID"}}]},{"fullName":["van Nieuwenhuizen C"],"firstName":["Chijs"],"lastName":["van Nieuwenhuizen"],"initials":["C"]},{"fullName":["Sabbe B"],"firstName":["Bernard"],"lastName":["Sabbe"],"initials":["B"]},{"fullName":["Broekaert E"],"firstName":["Eric"],"lastName":["Broekaert"],"initials":["E"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1263-1059","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["20"],"journalIssueId":["1801020"],"dateOfPublication":["2011 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2011"],"printPublicationDate":["2011-02-01"],"journal":[{"title":["Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation"],"ISOAbbreviation":["Qual Life Res"],"medlineAbbreviation":["Qual Life Res"],"NLMid":["9210257"],"ISSN":["0962-9343"],"ESSN":["1573-2649"]}]}],"pubYear":["2011"],"pageInfo":["139-150"],"abstractText":[" PURPOSE: This study explores the current QoL of opiate-dependent individuals who started outpatient methadone treatment at least 5 years ago and assesses the influence of demographic, psychosocial, drug and health-related variables on individuals' QoL. METHODS: Participants (n = 159) were interviewed about their current QoL, psychological distress and severity of drug-related problems, using the Lancashire Quality of Life Profile, the Brief Symptom Inventory and the Addiction Severity Index. Potential determinants of QoL were assessed in a multiple linear regression analysis. RESULTS: Five years after the start of methadone treatment, opiate-dependent individuals report low QoL scores on various domains. No association was found between drug-related variables and QoL, but a significant negative impact of psychological distress was identified. Severity of psychological distress, taking medication for psychological problems and the inability to change one's living situation were associated with lower QoL. Having at least one good friend and a structured daily activity had a significant, positive impact on QoL. CONCLUSIONS: Opiate-dependent individuals' QoL is mainly determined by their psychological well-being and a number of psychosocial variables. These findings highlight the importance of a holistic approach to treatment and support in methadone maintenance treatment, which goes beyond fixing the negative physical consequences of opiate dependence."],"affiliation":["Department of Orthopedagogics, Ghent University, H Dunantlaan 2, 9000 Ghent, Belgium. 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Flavivirus replication is based on endoplasmic reticulum-derived structures. Membrane remodeling and accumulation of viral factors induce endoplasmic reticulum stress that results in activation of a cellular signaling response termed unfolded protein response (UPR), which can be modulated by the viruses for their own benefit. Concomitant with the activation of the UPR, an upregulation of the autophagic pathway in cells infected with different flaviviruses has also been described. This review addresses the current knowledge of the relationship between endoplasmic reticulum stress, UPR, and autophagy in flavivirus-infected cells and the growing evidences for an involvement of these cellular pathways in the replication and pathogenesis of these viruses."],"affiliation":["Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Dengue virus","Virus replication","Unfolded protein response","West Nile virus","Autophagy","endoplasmic reticulum stress","Flavivirus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4042264?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4042264"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24917859"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24917859&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2014.00266"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["12"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-06-24"],"dateOfCreation":["2014-06-11"],"dateOfRevision":["2014-06-24"],"electronicPublicationDate":["2014-06-03"],"firstPublicationDate":["2014-06-03"],"cprojectID":"PMC4042264"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoihOUS2orL1IOf","_score":1,"_source":{"id":["25774068"],"source":["MED"],"pmid":["25774068"],"pmcid":["PMC4355442"],"doi":["10.1007/s10447-014-9225-0"],"title":["The Effect of Group Logotherapy on Meaning in Life and Depression Levels of Iranian Students."],"authorString":["Robatmili S, Sohrabi F, Shahrak MA, Talepasand S, Nokani M, Hasani M."],"authorList":[{"author":[{"fullName":["Robatmili S"],"firstName":["Somaye"],"lastName":["Robatmili"],"initials":["S"],"affiliation":["Department of Clinical Psychology, Allameh Tabataba'i University, Tehran, Iran."]},{"fullName":["Sohrabi F"],"firstName":["Faramarz"],"lastName":["Sohrabi"],"initials":["F"],"affiliation":["Department of Clinical Psychology, Allameh Tabataba'i University, Tehran, Iran."]},{"fullName":["Shahrak MA"],"firstName":["Mohammad Ali"],"lastName":["Shahrak"],"initials":["MA"],"affiliation":["Head of Counseling Center of Amir Kabir University of Technology, Tehran, Iran."]},{"fullName":["Talepasand S"],"firstName":["Siavash"],"lastName":["Talepasand"],"initials":["S"],"affiliation":["Department of Educational Psychology, Semnan University, Semnan, Iran."]},{"fullName":["Nokani M"],"firstName":["Mostafa"],"lastName":["Nokani"],"initials":["M"],"affiliation":["Department of Psychiatry, Medical Sciences University, Arak, Iran."]},{"fullName":["Hasani M"],"firstName":["Mohaddese"],"lastName":["Hasani"],"initials":["M"],"affiliation":["Department of Psychology, Zahedshahr Branch, Islamic Azad University, Zahedshahr, Iran."]}]}],"journalInfo":[{"issue":["1"],"volume":["37"],"journalIssueId":["2264325"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["International journal for the advancement of counseling"],"ISOAbbreviation":["Int J Adv Couns"],"medlineAbbreviation":["Int J Adv Couns"],"NLMid":["8219646"],"ISSN":["0165-0653"]}]}],"pubYear":["2015"],"pageInfo":["54-62"],"abstractText":["This paper identifies the effectiveness of group logotherapy in reducing depression and increasing meaning in life levels of university students in Iran. A randomized controlled trial was conducted with a pre- post- and follow-up test design. The instruments used were the Purpose in Life (PIL) test and the Beck Depression Inventory (BDI). Data were collected from 10 subjects in an experimental group and 10 in a control group. The experimental group participated in 10 sessions of group logotherapy, whilst the control group received no intervention. The mean scores for depression levels was significantly lower in the experimental group than in the control group and significantly higher in regard to meaning in life. Results suggest that group logotherapy has the potential to reduce depression levels and improve the meaning in life of university students."],"affiliation":["Department of Clinical Psychology, Allameh Tabataba'i University, Tehran, Iran."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Depression","Meaning In Life","Logotherapy","Iranian Students"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4355442?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4355442"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s10447-014-9225-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-03-16"],"dateOfRevision":["2015-03-18"],"firstPublicationDate":["2015-01-01"],"cprojectID":"PMC4355442"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqqv_8VqgcuJwvgV","_score":1,"_source":{"id":["25310102"],"source":["MED"],"pmid":["25310102"],"pmcid":["PMC4195678"],"doi":["10.1371/journal.pone.0109442"],"title":["Zika virus emergence in mosquitoes in southeastern Senegal, 2011."],"authorString":["Diallo D, Sall AA, Diagne CT, Faye O, Faye O, Ba Y, Hanley KA, Buenemann M, Weaver SC, Diallo M."],"authorList":[{"author":[{"fullName":["Diallo D"],"firstName":["Diawo"],"lastName":["Diallo"],"initials":["D"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Sall AA"],"firstName":["Amadou A"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Diagne CT"],"firstName":["Cheikh T"],"lastName":["Diagne"],"initials":["CT"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Ba Y"],"firstName":["Yamar"],"lastName":["Ba"],"initials":["Y"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal."]},{"fullName":["Hanley KA"],"firstName":["Kathryn A"],"lastName":["Hanley"],"initials":["KA"],"affiliation":["Department of Biology, New Mexico State University, Las Cruces, New Mexico, United States of America."]},{"fullName":["Buenemann M"],"firstName":["Michaela"],"lastName":["Buenemann"],"initials":["M"],"affiliation":["Department of Geography, New Mexico State University, Las Cruces, New Mexico, United States of America."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America."]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal."]}]}],"journalInfo":[{"issue":["10"],"volume":["9"],"journalIssueId":["2206285"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2014"],"pageInfo":["e109442"],"abstractText":["Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Spillover into humans has been documented in both regions and the virus is currently responsible for a large outbreak in French Polynesia. ZIKV amplifications are frequent in southeastern Senegal but little is known about their seasonal and spatial dynamics. The aim of this paper is to describe the spatio-temporal patterns of the 2011 ZIKV amplification in southeastern Senegal.Mosquitoes were collected monthly from April to December 2011 except during July. Each evening from 18:00 to 21:00 hrs landing collections were performed by teams of 3 persons working simultaneously in forest (canopy and ground), savannah, agriculture, village (indoor and outdoor) and barren land cover sites. Mosquitoes were tested for virus infection by virus isolation and RT-PCR. ZIKV was detected in 31 of the 1,700 mosquito pools (11,247 mosquitoes) tested: Ae. furcifer (5), Ae. luteocephalus (5), Ae. africanus (5), Ae. vittatus (3), Ae. taylori, Ae. dalzieli, Ae. hirsutus and Ae. metallicus (2 each) and Ae. aegypti, Ae. unilinaetus, Ma. uniformis, Cx. perfuscus and An. coustani (1 pool each) collected in June (3), September (10), October (11), November (6) and December (1). ZIKV was detected from mosquitoes collected in all land cover classes except indoor locations within villages. The virus was detected in only one of the ten villages investigated.This ZIKV amplification was widespread in the Kédougou area, involved several mosquito species as probable vectors, and encompassed all investigated land cover classes except indoor locations within villages. Aedes furcifer males and Aedes vittatus were found infected within a village, thus these species are probably involved in the transmission of Zika virus to humans in this environment."],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["AI069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["5P20RR016480-12"],"agency":["NCRR NIH HHS"],"acronym":["RR"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Senegal"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4195678?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4195678"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0109442"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["9"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-06-29"],"dateOfCreation":["2014-10-14"],"dateOfRevision":["2016-02-03"],"electronicPublicationDate":["2014-10-13"],"firstPublicationDate":["2014-10-13"],"cprojectID":"PMC4195678"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqrP_8VqgcuJwvgW","_score":1,"_source":{"id":["25360782"],"source":["MED"],"pmid":["25360782"],"pmcid":["PMC4215987"],"doi":["10.1371/journal.pone.0111224"],"title":["Zimbabwe culture before Mapungubwe: new evidence from Mapela Hill, South-Western Zimbabwe."],"authorString":["Chirikure S, Manyanga M, Pollard AM, Bandama F, Mahachi G, Pikirayi I."],"authorList":[{"author":[{"fullName":["Chirikure S"],"firstName":["Shadreck"],"lastName":["Chirikure"],"initials":["S"],"affiliation":["Department of Archaeology, University of Cape Town, Cape Town, South Africa."]},{"fullName":["Manyanga M"],"firstName":["Munyaradzi"],"lastName":["Manyanga"],"initials":["M"],"affiliation":["Department of History, University of Zimbabwe, Harare, Zimbabwe."]},{"fullName":["Pollard AM"],"firstName":["A Mark"],"lastName":["Pollard"],"initials":["AM"],"affiliation":["Research Laboratory for Archaeology and the History of Art, Oxford University, Oxford, United Kingdom."]},{"fullName":["Bandama F"],"firstName":["Foreman"],"lastName":["Bandama"],"initials":["F"],"affiliation":["Department of Archaeology, University of Cape Town, Cape Town, South Africa."]},{"fullName":["Mahachi G"],"firstName":["Godfrey"],"lastName":["Mahachi"],"initials":["G"],"affiliation":["National Museums and Monuments of Zimbabwe, Harare, Zimbabwe."]},{"fullName":["Pikirayi I"],"firstName":["Innocent"],"lastName":["Pikirayi"],"initials":["I"],"affiliation":["Department of Archaeology and Anthropology, University of Pretoria, Pretoria, South Africa."]}]}],"journalInfo":[{"issue":["10"],"volume":["9"],"journalIssueId":["2206285"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2014"],"pageInfo":["e111224"],"abstractText":["Across the globe, the emergence of complex societies excites intense academic debate in archaeology and allied disciplines. Not surprisingly, in southern Africa the traditional assumption that the evolution of socio-political complexity began with ideological transformations from K2 to Mapungubwe between CE1200 and 1220 is clouded in controversy. It is believed that the K2-Mapungubwe transitions crystallised class distinction and sacred leadership, thought to be the key elements of the Zimbabwe culture on Mapungubwe Hill long before they emerged anywhere else. From Mapungubwe (CE1220-1290), the Zimbabwe culture was expressed at Great Zimbabwe (CE1300-1450) and eventually Khami (CE1450-1820). However, new fieldwork at Mapela Hill, when coupled with a Bayesian chronology, offers tremendous fresh insights which refute this orthodoxy. Firstly, Mapela possesses enormous prestige stone-walled terraces whose initial construction date from the 11th century CE, almost two hundred years earlier than Mapungubwe. Secondly, the basal levels of the Mapela terraces and hilltop contain élite solid dhaka (adobe) floors associated with K2 pottery and glass beads. Thirdly, with a hilltop and flat area occupation since the 11th century CE, Mapela exhibits evidence of class distinction and sacred leadership earlier than K2 and Mapungubwe, the supposed propagators of the Zimbabwe culture. Fourthly, Mapungubwe material culture only appeared later in the Mapela sequence and therefore post-dates the earliest appearance of stone walling and dhaka floors at the site. Since stone walls, dhaka floors and class distinction are the essence of the Zimbabwe culture, their earlier appearance at Mapela suggests that Mapungubwe can no longer be regarded as the sole cradle of the Zimbabwe culture. This demands not just fresh ways of accounting for the rise of socio-political complexity in southern Africa, but also significant adjustments to existing models."],"affiliation":["Department of Archaeology, University of Cape Town, Cape Town, South Africa."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Glass"]},{"majorTopic_YN":["N"],"descriptorName":["Bayes Theorem"]},{"majorTopic_YN":["Y"],"descriptorName":["Archaeology"]},{"majorTopic_YN":["Y"],"descriptorName":["Culture"]},{"majorTopic_YN":["N"],"descriptorName":["Zimbabwe"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EH"],"qualifierName":["ethnology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Radiometric Dating"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4215987?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4215987"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0111224"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-06-25"],"dateOfCreation":["2014-11-01"],"electronicPublicationDate":["2014-10-31"],"firstPublicationDate":["2014-10-31"],"cprojectID":"PMC4215987"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqxoJ9hGurOzZIYo","_score":1,"_source":{"id":["26492265"],"source":["MED"],"pmid":["26492265"],"pmcid":["PMC4632385"],"doi":["10.3390/v7102877"],"title":["Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques."],"authorString":["Parkash O, Shueb RH."],"authorList":[{"author":[{"fullName":["Parkash O"],"firstName":["Om"],"lastName":["Parkash"],"initials":["O"],"affiliation":["Department of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. om.aprembhagat@gmail.com."]},{"fullName":["Shueb RH"],"firstName":["Rafidah Hanim"],"lastName":["Shueb"],"initials":["RH"],"affiliation":["Department of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. hanimshueb@gmail.com."]}]}],"journalInfo":[{"issue":["10"],"volume":["7"],"journalIssueId":["2346027"],"dateOfPublication":["2015 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2015"],"printPublicationDate":["2015-10-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2015"],"pageInfo":["5410-5427"],"abstractText":["Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed."],"affiliation":["Department of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. om.aprembhagat@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Clinical Laboratory Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Biosensing Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]}]}],"keywordList":[{"keyword":["RT-PCR","Biosensor","Detection","Diagnosis","NS1","Dengue","Dengue Specific Igm","Viral Isolation"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4632385?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4632385"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v7102877"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-22"],"dateOfCreation":["2015-10-23"],"dateOfRevision":["2015-11-24"],"electronicPublicationDate":["2015-10-19"],"firstPublicationDate":["2015-10-19"],"cprojectID":"PMC4632385"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq94hOUS2orL1IPQ","_score":1,"_source":{"id":["26934531"],"source":["MED"],"pmid":["26934531"],"pmcid":["PMC4774925"],"doi":["10.1371/journal.pntd.0004530"],"title":["Zika Virus: Medical Countermeasure Development Challenges."],"authorString":["Malone RW, Homan J, Callahan MV, Glasspool-Malone J, Damodaran L, Schneider Ade B, Zimler R, Talton J, Cobb RR, Ruzic I, Smith-Gagen J, Janies D, Wilson J, Zika Response Working Group."],"authorList":[{"author":[{"fullName":["Malone RW"],"firstName":["Robert W"],"lastName":["Malone"],"initials":["RW"],"affiliation":["Class of 2016, Harvard Medical School Global Clinical Scholars Research Training Program, Boston, Massachusetts, United States of America."]},{"fullName":["Homan J"],"firstName":["Jane"],"lastName":["Homan"],"initials":["J"],"affiliation":["ioGenetics, Madison, Wisconsin, United States of America."]},{"fullName":["Callahan MV"],"firstName":["Michael V"],"lastName":["Callahan"],"initials":["MV"],"affiliation":["Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America."]},{"fullName":["Glasspool-Malone J"],"firstName":["Jill"],"lastName":["Glasspool-Malone"],"initials":["J"],"affiliation":["Class of 2016, Harvard Medical School Global Clinical Scholars Research Training Program, Boston, Massachusetts, United States of America."]},{"fullName":["Damodaran L"],"firstName":["Lambodhar"],"lastName":["Damodaran"],"initials":["L"],"affiliation":["Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina, United States of America."]},{"fullName":["Schneider Ade B"],"firstName":["Adriano De Bernardi"],"lastName":["Schneider"],"initials":["Ade B"],"affiliation":["Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina, United States of America."]},{"fullName":["Zimler R"],"firstName":["Rebecca"],"lastName":["Zimler"],"initials":["R"],"affiliation":["University of Florida, Department of Entomology and Nematology, Florida Medical Entomology Laboratory, Vero Beach, Florida, United States of America."]},{"fullName":["Talton J"],"firstName":["James"],"lastName":["Talton"],"initials":["J"],"affiliation":["Nanotherapeutics, NANO-ADM Advanced Development and Manufacturing Center, Alachua, Florida, United States of America."]},{"fullName":["Cobb RR"],"firstName":["Ronald R"],"lastName":["Cobb"],"initials":["RR"],"affiliation":["Nanotherapeutics, NANO-ADM Advanced Development and Manufacturing Center, Alachua, Florida, United States of America."]},{"fullName":["Ruzic I"],"firstName":["Ivan"],"lastName":["Ruzic"],"initials":["I"],"affiliation":["Analytical Outcomes, Washington Crossing, Pennsylvania, United States of America."]},{"fullName":["Smith-Gagen J"],"firstName":["Julie"],"lastName":["Smith-Gagen"],"initials":["J"],"affiliation":["School of Community Health Sciences, University of Nevada, Reno, Nevada, United States of America."]},{"fullName":["Janies D"],"firstName":["Daniel"],"lastName":["Janies"],"initials":["D"],"affiliation":["Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina, United States of America."]},{"fullName":["Wilson J"],"firstName":["James"],"lastName":["Wilson"],"initials":["J"],"affiliation":["Nevada Center for Infectious Disease Forecasting, University of Nevada, Reno, Nevada, United States of America."]},{"collectiveName":["Zika Response Working Group"]}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004530"],"abstractText":["Reports of high rates of primary microcephaly and Guillain-Barré syndrome associated with Zika virus infection in French Polynesia and Brazil have raised concerns that the virus circulating in these regions is a rapidly developing neuropathic, teratogenic, emerging infectious public health threat. There are no licensed medical countermeasures (vaccines, therapies or preventive drugs) available for Zika virus infection and disease. The Pan American Health Organization (PAHO) predicts that Zika virus will continue to spread and eventually reach all countries and territories in the Americas with endemic Aedes mosquitoes. This paper reviews the status of the Zika virus outbreak, including medical countermeasure options, with a focus on how the epidemiology, insect vectors, neuropathology, virology and immunology inform options and strategies available for medical countermeasure development and deployment.Multiple information sources were employed to support the review. These included publically available literature, patents, official communications, English and Lusophone lay press. Online surveys were distributed to physicians in the US, Mexico and Argentina and responses analyzed. Computational epitope analysis as well as infectious disease outbreak modeling and forecasting were implemented. Field observations in Brazil were compiled and interviews conducted with public health officials."],"affiliation":["Class of 2016, Harvard Medical School Global Clinical Scholars Research Training Program, Boston, Massachusetts, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Disease Control"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Americas"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Polynesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Transmission, Infectious"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Pandemics"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4774925?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4774925"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004530"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-03-03"],"dateOfRevision":["2016-03-11"],"electronicPublicationDate":["2016-03-02"],"firstPublicationDate":["2016-03-02"],"cprojectID":"PMC4774925"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrAQ_8VqgcuJwvhH","_score":1,"_source":{"id":["26821709"],"source":["MED"],"pmid":["26821709"],"pmcid":["PMC4730666"],"doi":["10.1186/s12936-016-1100-5"],"title":["Concurrent malaria and arbovirus infections in Kedougou, southeastern Senegal."],"authorString":["Sow A, Loucoubar C, Diallo D, Faye O, Ndiaye Y, Senghor CS, Dia AT, Faye O, Weaver SC, Diallo M, Malvy D, Sall AA."],"authorList":[{"author":[{"fullName":["Sow A"],"firstName":["Abdourahmane"],"lastName":["Sow"],"initials":["A"],"affiliation":["Institut de Santé Publique d'Epidémiologie et de Développement (ISPED), Centre de recherche INSERM U897 Epidémiologie-Biostatistique, Université de Bordeaux, Bordeaux, France. asow@pasteur.sn."]},{"fullName":["Loucoubar C"],"firstName":["Cheikh"],"lastName":["Loucoubar"],"initials":["C"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. cloucoubar@pasteur.sn."]},{"fullName":["Diallo D"],"firstName":["Diawo"],"lastName":["Diallo"],"initials":["D"],"affiliation":["Medical Entomology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. diawod@yahoo.fr."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. oumarfaye@pasteur.sn."]},{"fullName":["Ndiaye Y"],"firstName":["Youssoupha"],"lastName":["Ndiaye"],"initials":["Y"],"affiliation":["Saraya Health District, Saraya, Senegal. youndiaye@hotmail.com."]},{"fullName":["Senghor CS"],"firstName":["Cheikh Saadibou"],"lastName":["Senghor"],"initials":["CS"],"affiliation":["Kedougou Health District, Kedougou, Senegal. doczenghos@yahoo.fr."]},{"fullName":["Dia AT"],"firstName":["Anta Tal"],"lastName":["Dia"],"initials":["AT"],"affiliation":["Institut Santé et développement (ISED), Université Cheikh Anta Diop, Dakar, Senegal. adia@ised.sn."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. ofaye@pasteur.sn."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Department of Pathology, Institute for Human Infections and Immunity, Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX, USA. sweaver@utmb.edu."]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"],"affiliation":["Medical Entomology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. diallo@pasteur.sn."]},{"fullName":["Malvy D"],"firstName":["Denis"],"lastName":["Malvy"],"initials":["D"],"affiliation":["Institut de Santé Publique d'Epidémiologie et de Développement (ISPED), Centre de recherche INSERM U897 Epidémiologie-Biostatistique, Université de Bordeaux, Bordeaux, France. denis.malvy@chu-bordeaux.fr."]},{"fullName":["Sall AA"],"firstName":["Amadou Alpha"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal. asall@pasteur.sn."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2401987"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Malaria journal"],"ISOAbbreviation":["Malar. J."],"medlineAbbreviation":["Malar J"],"NLMid":["101139802"],"ESSN":["1475-2875"]}]}],"pubYear":["2016"],"pageInfo":["47"],"abstractText":["Malaria is one of the leading causes of acute febrile illness (AFI) in Africa. With the advent of malaria rapid diagnostic tests, misdiagnosis and co-morbidity with other diseases has been highlighted by an increasing number of studies. Although arboviral infections and malaria are both vector-borne diseases and often have an overlapping geographic distribution in sub-Saharan Africa, information about their incidence rates and concurrent infections is scarce.From July 2009 to March 2013 patients from seven healthcare facilities of the Kedougou region presenting with AFI were enrolled and tested for malaria and arboviral infections, i.e., yellow fever (YFV), West Nile (WNV), dengue (DENV), chikungunya (CHIKV), Crimean Congo haemorrhagic fever (CCHFV), Zika (ZIKV), and Rift Valley fever viruses (RVFV). Malaria parasite infections were investigated using thick blood smear (TBS) and rapid diagnostics tests (RDT) while arbovirus infections were tested by IgM antibody detection (ELISA) and RT-PCR assays. Data analysis of single or concurrent malaria and arbovirus was performed using R software.A total of 13,845 patients, including 7387 with malaria and 41 with acute arbovirus infections (12 YFV, nine ZIKV, 16 CHIKV, three DENV, and one RVFV) were enrolled. Among the arbovirus-infected patients, 48.7% (20/41) were co-infected with malaria parasites at the following frequencies: CHIKV 18.7% (3/16), YFV 58.3% (7/12), ZIKV 88.9% (8/9), DENV 33.3% (1/3), and RVF 100% (1/1). Fever ≥40 °C was the only sign or symptom significantly associated with dual malaria parasite/arbovirus infection.Concurrent malaria parasite and arbovirus infections were detected in the Kedougou region from 2009 to 2013 and need to be further documented, including among asymptomatic individuals, to assess its epidemiological and clinical impact."],"affiliation":["Institut de Santé Publique d'Epidémiologie et de Développement (ISPED), Centre de recherche INSERM U897 Epidémiologie-Biostatistique, Université de Bordeaux, Bordeaux, France. asow@pasteur.sn."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["AI1069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4730666?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4730666"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12936-016-1100-5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-01-29"],"dateOfRevision":["2016-02-02"],"electronicPublicationDate":["2016-01-28"],"firstPublicationDate":["2016-01-28"],"cprojectID":"PMC4730666"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrBBJ9hGurOzZIZH","_score":1,"_source":{"id":["26983551"],"source":["MED"],"pmid":["26983551"],"pmcid":["PMC4794815"],"doi":["10.1186/s12961-016-0091-6"],"title":["Financing and collaboration on research and development for nodding syndrome."],"authorString":["Årdal C, Røttingen JA."],"authorList":[{"author":[{"fullName":["Årdal C"],"firstName":["Christine"],"lastName":["Årdal"],"initials":["C"],"affiliation":["Department of International Public Health, Norwegian Institute of Public Health, Oslo, Norway. christine.ardal@fhi.no."]},{"fullName":["Røttingen JA"],"firstName":["John-Arne"],"lastName":["Røttingen"],"initials":["JA"],"affiliation":["Department of Global Health and Population, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA."]}]}],"journalInfo":[{"volume":["14"],"journalIssueId":["2402729"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Health research policy and systems / BioMed Central"],"ISOAbbreviation":["Health Res Policy Syst"],"medlineAbbreviation":["Health Res Policy Syst"],"NLMid":["101170481"],"ESSN":["1478-4505"]}]}],"pubYear":["2016"],"pageInfo":["19"],"abstractText":["Nodding syndrome is a neurological disease with no known cure or treatment, impacting children aged 3-18 years old, mainly in East Africa. Children progressively develop varying degrees of cognitive impairment which may lead to severe wasting, a vegetative state and, eventually, death. Despite its 50-year existence, little is known about its cause, risk factors and prognosis. It is a disease where markets will not provide solutions because the patients are both too few and too poor, making it especially neglected. Open source innovation has been recommended as an approach to neglected disease research in order to maximize available funding through greater collaboration and openness to results. Nodding syndrome is a useful case to examine the relevance of open source innovation.We assessed the magnitude of research related to nodding syndrome, its availability, financing and the amount of collaboration. We surveyed researchers regarding their motivations, attitudes toward open source innovation concepts and barriers to greater collaboration.Little research is occurring for nodding syndrome, but it is openly available and researchers are highly collaborative. The disease is largely unknown, which is partly attributed to WHO not classifying nodding syndrome as a neglected tropical disease and not including it in any formal programme. Impacted countries, particularly Uganda, demonstrate a strong degree of ownership through both authorship and research financing. Nodding syndrome researchers have been allocated a total of €5 million from 2013 to 2019 in grant funding. Annual financing, due to three new grants, doubled from 2014 to 2015.Nodding syndrome, a disease previously ignored by the international community, is starting to receive greater attention, although financing remains modest. If infectious, a larger epidemic could take the world by surprise. Open source innovation can likely help by sharing research protocols (to avoid duplication) and early research results (to adjust to the findings of others). The existing scientists have already endorsed open source innovation, but increased financing is needed. The support of just a few high-income countries could reap a large impact."],"affiliation":["Department of International Public Health, Norwegian Institute of Public Health, Oslo, Norway. christine.ardal@fhi.no."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Neglected Tropical Diseases","Open Science","Open Source Drug Discovery","Nodding Syndrome","Market Failures","Open Knowledge Innovation","Open Source Innovation"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4794815?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4794815"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12961-016-0091-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCreation":["2016-03-17"],"dateOfRevision":["2016-03-19"],"electronicPublicationDate":["2016-03-17"],"firstPublicationDate":["2016-03-17"],"cprojectID":"PMC4794815"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqzzhOUS2orL1IO2","_score":1,"_source":{"id":["26492495"],"source":["MED"],"pmid":["26492495"],"pmcid":["PMC4619608"],"doi":["10.1371/journal.ppat.1005182"],"title":["Preparing for the Next Epidemic with Basic Virology."],"authorString":["Diamond MS."],"authorList":[{"author":[{"fullName":["Diamond MS"],"firstName":["Michael S"],"lastName":["Diamond"],"initials":["MS"],"affiliation":["Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America; Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, Missouri, United States of America."]}]}],"journalInfo":[{"issue":["10"],"volume":["11"],"journalIssueId":["2328613"],"dateOfPublication":["2015 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2015"],"printPublicationDate":["2015-10-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2015"],"pageInfo":["e1005182"],"affiliation":["Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America; Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, United States of America; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, Missouri, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["West Nile Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immune Evasion"]},{"majorTopic_YN":["N"],"descriptorName":["Epidemics"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4619608?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4619608"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005182"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-11"],"dateOfCreation":["2015-10-23"],"dateOfRevision":["2015-10-30"],"electronicPublicationDate":["2015-10-22"],"firstPublicationDate":["2015-10-22"],"cprojectID":"PMC4619608"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqznhOUS2orL1IO1","_score":1,"_source":{"id":["26406240"],"source":["MED"],"pmid":["26406240"],"pmcid":["PMC4583371"],"doi":["10.1371/journal.pntd.0004100"],"title":["Value of Routine Dengue Diagnostic Tests in Urine and Saliva Specimens."],"authorString":["Andries AC, Duong V, Ly S, Cappelle J, Kim KS, Lorn Try P, Ros S, Ong S, Huy R, Horwood P, Flamand M, Sakuntabhai A, Tarantola A, Buchy P."],"authorList":[{"author":[{"fullName":["Andries AC"],"firstName":["Anne-Claire"],"lastName":["Andries"],"initials":["AC"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."]},{"fullName":["Duong V"],"firstName":["Veasna"],"lastName":["Duong"],"initials":["V"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."]},{"fullName":["Ly S"],"firstName":["Sowath"],"lastName":["Ly"],"initials":["S"],"affiliation":["Institut Pasteur in Cambodia, Epidemiology and Public Health Unit, Phnom Penh, Cambodia."]},{"fullName":["Cappelle J"],"firstName":["Julien"],"lastName":["Cappelle"],"initials":["J"],"affiliation":["Institut Pasteur in Cambodia, Epidemiology and Public Health Unit, Phnom Penh, Cambodia; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Unité AGIRs, Montpellier, France."]},{"fullName":["Kim KS"],"firstName":["Kim Srorn"],"lastName":["Kim"],"initials":["KS"],"affiliation":["Kampong Cham Provincial Hospital, Pediatric Department, Kampong Cham, Cambodia."]},{"fullName":["Lorn Try P"],"firstName":["Patrich"],"lastName":["Lorn Try"],"initials":["P"],"affiliation":["Kampong Cham Provincial Hospital, Pediatric Department, Kampong Cham, Cambodia."]},{"fullName":["Ros S"],"firstName":["Sopheaktra"],"lastName":["Ros"],"initials":["S"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."]},{"fullName":["Ong S"],"firstName":["Sivuth"],"lastName":["Ong"],"initials":["S"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."]},{"fullName":["Huy R"],"firstName":["Rekol"],"lastName":["Huy"],"initials":["R"],"affiliation":["Ministry of Health, Centre National de Malariologie, Phnom Penh, Cambodia."]},{"fullName":["Horwood P"],"firstName":["Paul"],"lastName":["Horwood"],"initials":["P"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."]},{"fullName":["Flamand M"],"firstName":["Marie"],"lastName":["Flamand"],"initials":["M"],"affiliation":["Institut Pasteur, Structural Virology Unit & CNRS UMR 3569, Paris, France."]},{"fullName":["Sakuntabhai A"],"firstName":["Anavaj"],"lastName":["Sakuntabhai"],"initials":["A"],"affiliation":["Institut Pasteur, Functional Genetics of Infectious Diseases Unit, Paris, France; Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, Paris, France."]},{"fullName":["Tarantola A"],"firstName":["Arnaud"],"lastName":["Tarantola"],"initials":["A"],"authorId":[{"_":"0000-0002-6946-7958","$":{"type":"ORCID"}}],"affiliation":["Institut Pasteur in Cambodia, Epidemiology and Public Health Unit, Phnom Penh, Cambodia."]},{"fullName":["Buchy P"],"firstName":["Philippe"],"lastName":["Buchy"],"initials":["P"],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia; GlaxoSmithKline Vaccines, Vaccine Value and Health Sciences, Singapore, Singapore."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6946-7958","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["9"],"volume":["9"],"journalIssueId":["2318589"],"dateOfPublication":["2015 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2015"],"printPublicationDate":["2015-09-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004100"],"abstractText":["Dengue laboratory diagnosis is essentially based on detection of the virus, its components or antibodies directed against the virus in blood samples. Blood, however, may be difficult to draw in some patients, especially in children, and sampling during outbreak investigations or epidemiological studies may face logistical challenges or limited compliance to invasive procedures from subjects. The aim of this study was to assess the possibility of using saliva and urine samples instead of blood for dengue diagnosis.Serial plasma, urine and saliva samples were collected at several time-points between the day of admission to hospital until three months after the onset of fever in children with confirmed dengue disease. Quantitative RT-PCR, NS1 antigen capture and ELISA serology for anti-DENV antibody (IgG, IgM and IgA) detection were performed in parallel on the three body fluids. RT-PCR and NS1 tests demonstrated an overall sensitivity of 85.4%/63.4%, 41.6%/14.5% and 39%/28.3%, in plasma, urine and saliva specimens, respectively. When urine and saliva samples were collected at the same time-points and tested concurrently, the diagnostic sensitivity of RNA and NS1 detection assays was 69.1% and 34.4%, respectively. IgG/IgA detection assays had an overall sensitivity of 54.4%/37.4%, 38.5%/26.8% and 52.9%/28.6% in plasma, urine and saliva specimens, respectively. IgM were detected in 38.1% and 36% of the plasma and saliva samples but never in urine.Although the performances of the different diagnostic methods were not as good in saliva and urine as in plasma specimens, the results obtained by qRT-PCR and by anti-DENV antibody ELISA could well justify the use of these two body fluids to detect dengue infection in situations when the collection of blood specimens is not possible."],"affiliation":["Institut Pasteur in Cambodia, Virology Unit, Phnom Penh, Cambodia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Saliva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Plasma"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Urine"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["UR"],"qualifierName":["urine"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Progression"]},{"majorTopic_YN":["N"],"descriptorName":["Viral Nonstructural 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virus"],"registryNumber":["0"]},{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["RNA, Viral"],"registryNumber":["0"]},{"name":["Immunoglobulin Isotypes"],"registryNumber":["0"]},{"name":["Viral Nonstructural Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4583371?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4583371"]},{"availability":["Subscription 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnq4RhOUS2orL1IO-","_score":1,"_source":{"id":["26610350"],"source":["MED"],"pmid":["26610350"],"pmcid":["PMC4661982"],"doi":["10.1186/s12861-015-0092-7"],"title":["3D structured illumination microscopy of mammalian embryos and spermatozoa."],"authorString":["Popken J, Dahlhoff M, Guengoer T, Wolf E, Zakhartchenko V."],"authorList":[{"author":[{"fullName":["Popken J"],"firstName":["Jens"],"lastName":["Popken"],"initials":["J"],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, D-81377, Munich, Germany. Jens.Popken@campus.lmu.de."]},{"fullName":["Dahlhoff M"],"firstName":["Maik"],"lastName":["Dahlhoff"],"initials":["M"],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, D-81377, Munich, Germany. dahlhoff@lmb.uni-muenchen.de."]},{"fullName":["Guengoer T"],"firstName":["Tuna"],"lastName":["Guengoer"],"initials":["T"],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, D-81377, Munich, Germany. T.Guengoer@gen.vetmed.uni-muenchen.de."]},{"fullName":["Wolf E"],"firstName":["Eckhard"],"lastName":["Wolf"],"initials":["E"],"authorId":[{"_":"0000-0002-0430-9510","$":{"type":"ORCID"}}],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, D-81377, Munich, Germany. ewolf@lmu.de."]},{"fullName":["Zakhartchenko V"],"firstName":["Valeri"],"lastName":["Zakhartchenko"],"initials":["V"],"authorId":[{"_":"0000-0002-5974-9759","$":{"type":"ORCID"}}],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, LMU Munich, Hackerstr. 27, D-85764, Oberschleissheim, Germany. v.zakhartchenko@gen.vetmed.uni-muenchen.de."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0430-9510","$":{"type":"ORCID"}},{"_":"0000-0002-5974-9759","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2261392"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BMC developmental biology"],"ISOAbbreviation":["BMC Dev. Biol."],"medlineAbbreviation":["BMC Dev Biol"],"NLMid":["100966973"],"ESSN":["1471-213X"]}]}],"pubYear":["2015"],"pageInfo":["46"],"abstractText":["Super-resolution fluorescence microscopy performed via 3D structured illumination microscopy (3D-SIM) is well established on flat, adherent cells. However, blastomeres of mammalian embryos are non-adherent, round and large. Scanning whole mount mammalian embryos with 3D-SIM is prone to failure due to the movement during scanning and the large distance to the cover glass.Here we present a highly detailed protocol that allows performing 3D-SIM on blastomeres of mammalian embryos with an image quality comparable to scans in adherent cells. This protocol was successfully tested on mouse, rabbit and cattle embryos and on rabbit spermatozoa.Our protocol provides detailed instructions on embryo staining, blastomere isolation, blastomere attachment, embedding, correct oil predictions, scanning conditions, and oil correction choices after the first scan. Finally, the most common problems are documented and solutions are suggested. To our knowledge, this protocol presents for the first time a highly detailed and practical way to perform 3D-SIM on mammalian embryos and spermatozoa."],"affiliation":["Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, D-81377, Munich, Germany. Jens.Popken@campus.lmu.de."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Spermatozoa"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Blastomeres"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Cattle"]},{"majorTopic_YN":["N"],"descriptorName":["Rabbits"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Imaging, Three-Dimensional"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Microscopy, Confocal"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Microscopy, Fluorescence"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Staining and Labeling"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Tissue Embedding"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Tissue Fixation"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Embryo, Mammalian"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4661982?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4661982"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12861-015-0092-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2015-11-27"],"dateOfRevision":["2015-11-30"],"electronicPublicationDate":["2015-11-26"],"firstPublicationDate":["2015-11-26"],"cprojectID":"PMC4661982"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq4DhOUS2orL1IO9","_score":1,"_source":{"id":["26641858"],"source":["MED"],"pmid":["26641858"],"pmcid":["PMC4671560"],"doi":["10.1371/journal.pntd.0004250"],"title":["Molecular Differentiation of the African Yellow Fever Vector Aedes bromeliae (Diptera: Culicidae) from Its Sympatric Non-vector Sister Species, Aedes lilii."],"authorString":["Bennett KL, Linton YM, Shija F, Kaddumukasa M, Djouaka R, Misinzo G, Lutwama J, Huang YM, Mitchell LB, Richards M, Tossou E, Walton C."],"authorList":[{"author":[{"fullName":["Bennett KL"],"firstName":["Kelly Louise"],"lastName":["Bennett"],"initials":["KL"],"affiliation":["Computational Evolutionary Biology Group, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom."]},{"fullName":["Linton YM"],"firstName":["Yvonne-Marie"],"lastName":["Linton"],"initials":["YM"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America."]},{"fullName":["Shija F"],"firstName":["Fortunate"],"lastName":["Shija"],"initials":["F"],"affiliation":["Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro, Tanzania."]},{"fullName":["Kaddumukasa M"],"firstName":["Martha"],"lastName":["Kaddumukasa"],"initials":["M"],"affiliation":["Department of Arbovirology, Emerging and Re-emerging Infections, Uganda Virus Research Institute, Entebbe, Uganda."]},{"fullName":["Djouaka R"],"firstName":["Rousseau"],"lastName":["Djouaka"],"initials":["R"],"affiliation":["Agro-Eco-Health Platform for West and Central Africa, International Institute for Tropical Agriculture, Republic of Benin."]},{"fullName":["Misinzo G"],"firstName":["Gerald"],"lastName":["Misinzo"],"initials":["G"],"affiliation":["Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro, Tanzania."]},{"fullName":["Lutwama J"],"firstName":["Julius"],"lastName":["Lutwama"],"initials":["J"],"affiliation":["Department of Arbovirology, Emerging and Re-emerging Infections, Uganda Virus Research Institute, Entebbe, Uganda."]},{"fullName":["Huang YM"],"firstName":["Yiau-Min"],"lastName":["Huang"],"initials":["YM"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America."]},{"fullName":["Mitchell LB"],"firstName":["Luke B"],"lastName":["Mitchell"],"initials":["LB"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America."]},{"fullName":["Richards M"],"firstName":["Miriam"],"lastName":["Richards"],"initials":["M"],"affiliation":["Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro, Tanzania."]},{"fullName":["Tossou E"],"firstName":["Eric"],"lastName":["Tossou"],"initials":["E"],"affiliation":["Agro-Eco-Health Platform for West and Central Africa, International Institute for Tropical Agriculture, Republic of Benin."]},{"fullName":["Walton C"],"firstName":["Catherine"],"lastName":["Walton"],"initials":["C"],"affiliation":["Computational Evolutionary Biology Group, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom."]}]}],"journalInfo":[{"issue":["12"],"volume":["9"],"journalIssueId":["2347466"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004250"],"abstractText":["Yellow fever continues to be a problem in sub-Saharan Africa with repeated epidemics occurring. The mosquito Aedes bromeliae is a major vector of yellow fever, but it cannot be readily differentiated from its non-vector zoophilic sister species Ae. lilii using morphological characters. Genetic differences have been reported between anthropophilic Ae. bromeliae and zoophilic Ae. lilii and between forest and domestic populations. However, due to the application of different molecular markers and non-overlapping populations employed in previous studies, interpretation of species delimitation is unclear.DNA sequences were generated from specimens of Ae. simpsoni s.l. from the Republic of Benin, Tanzania and Uganda for two nuclear genes apolipophorin 2 (apoLp2) and cytochrome p450 (CYPJ92), the ribosomal internal transcribed spacer region (ITS) and the mitochondrial cytochrome c oxidase (COI) barcoding region. Nuclear genes apoLp2 and CYPJ92 were unable to differentiate between species Ae. bromeliae and Ae. lilii due to ancestral lineage sorting, while ITS sequence data provided clear topological separation on a phylogeny. The standard COI barcoding region was shown to be subject to species introgression and unable to clearly distinguish the two taxa. Here we present a reliable direct PCR-based method for differentiation of the vector species Ae. bromeliae from its isomorphic, sympatric and non-biomedically important sister taxon, Ae. lilii, based on the ITS region. Using molecular species verification, we describe novel immature habitats for Ae. lilii and report both sympatric and allopatric populations. Whereas only Ae. lilii is found in the Republic of Benin and only Ae. bromeliae in Tanzania, both species are sympatric in Uganda.Our accurate identification method will allow informed distribution and detailed ecological studies that will facilitate assessment of arboviral disease risk and development of future targeted vector control."],"affiliation":["Computational Evolutionary Biology Group, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Ribosomal Spacer"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["N"],"descriptorName":["Entomology"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ecosystem"]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Tanzania"]},{"majorTopic_YN":["N"],"descriptorName":["Uganda"]},{"majorTopic_YN":["N"],"descriptorName":["Benin"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeography"]}]}],"chemicalList":[{"chemical":[{"name":["Insect Proteins"],"registryNumber":["0"]},{"name":["DNA, Ribosomal Spacer"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4671560?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4671560"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004250"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-04-06"],"dateOfCreation":["2015-12-08"],"dateOfRevision":["2015-12-14"],"electronicPublicationDate":["2015-12-07"],"firstPublicationDate":["2015-12-07"],"cprojectID":"PMC4671560"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrEkhOUS2orL1IPd","_score":1,"_source":{"id":["26982959"],"source":["MED"],"pmid":["26982959"],"pmcid":["PMC4794767"],"doi":["10.1590/s1518-8787.2016050006245"],"title":["Finding Aedes aegypti in a natural breeding site in an urban zone, Sao Paulo, Southeastern Brazil."],"authorString":["Lima-Camara TN, Urbinatti PR, Chiaravalloti-Neto F."],"authorList":[{"author":[{"fullName":["Lima-Camara TN"],"firstName":["Tamara Nunes"],"lastName":["Lima-Camara"],"initials":["TN"],"affiliation":["Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil."]},{"fullName":["Urbinatti PR"],"firstName":["Paulo Roberto"],"lastName":["Urbinatti"],"initials":["PR"],"affiliation":["Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil."]},{"fullName":["Chiaravalloti-Neto F"],"firstName":["Francisco"],"lastName":["Chiaravalloti-Neto"],"initials":["F"],"affiliation":["Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil."]}]}],"journalInfo":[{"volume":["50"],"journalIssueId":["2386504"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Revista de saude publica"],"ISOAbbreviation":["Rev Saude Publica"],"medlineAbbreviation":["Rev Saude Publica"],"NLMid":["0135043"],"ISSN":["0034-8910"],"ESSN":["1518-8787"]}]}],"pubYear":["2016"],"pageInfo":["3"],"abstractText":["This is the description of how nine Aedes aegypti larvae were found in a natural breeding site in the Pinheiros neighborhood, city of Sao Paulo, SP, Southeastern Brazil. The record was conducted in December 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. FindingAe. aegypti larvae in a tree hole shows this species' ability to use both artificial and natural environments as breeding sites and habitats, which points towards the importance of maintaining continuous surveillance on this mosquito in all kinds of water-holding containers."],"affiliation":["Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4794767?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4794767"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/S1518-8787.2016050006245"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-17"],"dateOfRevision":["2016-03-30"],"electronicPublicationDate":["2016-03-15"],"firstPublicationDate":["2016-03-15"],"cprojectID":"PMC4794767"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq8g_8VqgcuJwvg5","_score":1,"_source":{"id":["25524414"],"source":["MED"],"pmid":["25524414"],"pmcid":["PMC4580713"],"doi":["10.1007/s10943-014-9983-3"],"title":["The Role of Meaning in Life Within the Relations of Religious Coping and Psychological Well-Being."],"authorString":["Krok D."],"authorList":[{"author":[{"fullName":["Krok D"],"firstName":["Dariusz"],"lastName":["Krok"],"initials":["D"],"affiliation":["Institute of Family Sciences, Opole University, ul. Drzymały 1a, 35-342, Opole, Poland. dkrok@uni.opole.pl."]}]}],"journalInfo":[{"issue":["6"],"volume":["54"],"journalIssueId":["2326460"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["Journal of religion and health"],"ISOAbbreviation":["J Relig Health"],"medlineAbbreviation":["J Relig Health"],"NLMid":["2985199R"],"ISSN":["0022-4197"],"ESSN":["1573-6571"]}]}],"pubYear":["2015"],"pageInfo":["2292-2308"],"abstractText":["The purpose of this study was to examine whether meaning in life understood in terms of presence, search, and personal meaning is a mediator in the relationships between religious coping and psychological well-being. Associations of religiousness and psychological well-being are complex and suggest the existence of meaning and purpose in their internal structures. Two studies were conducted. In Study 1, presence of meaning in life was a mediator between negative coping and psychological well-being in the scope of a total score and all its dimensions. Search for meaning in life did not mediate the above relations. In Study 2, personal meaning turned out to be a partial mediator between negative coping and psychological well-being. These findings suggest that meaning in life is a crucial element of religious coping and psychological well-being that is used by people as a part of their meaning system to cope with life's difficulties and challenges."],"affiliation":["Institute of Family Sciences, Opole University, ul. Drzymały 1a, 35-342, Opole, Poland. dkrok@uni.opole.pl."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Psychological Well-being","Religious Coping","Meaning In Life","Purpose And Significance"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4580713?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4580713"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s10943-014-9983-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-09-24"],"dateOfRevision":["2015-10-02"],"firstPublicationDate":["2014-12-19"],"cprojectID":"PMC4580713"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnq7YJ9hGurOzZIY8","_score":1,"_source":{"id":["26966322"],"source":["MED"],"pmid":["26966322"],"pmcid":["PMC4773943"],"doi":["10.2471/blt.16.170860"],"title":["Data sharing in public health emergencies: a call to researchers."],"authorString":["Dye C, Bartolomeos K, Moorthy V, Kieny MP."],"authorList":[{"author":[{"fullName":["Dye C"],"firstName":["Christopher"],"lastName":["Dye"],"initials":["C"],"affiliation":["Department of Strategy, Policy & Information, World Health Organization, avenue Appia 20, Geneva 27, Switzerland ."]},{"fullName":["Bartolomeos K"],"firstName":["Kidist"],"lastName":["Bartolomeos"],"initials":["K"],"affiliation":["Department of Strategy, Policy & Information, World Health Organization, avenue Appia 20, Geneva 27, Switzerland ."]},{"fullName":["Moorthy V"],"firstName":["Vasee"],"lastName":["Moorthy"],"initials":["V"],"affiliation":["Department of Immunization, Vaccines & Biologicals, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Kieny MP"],"firstName":["Marie Paule"],"lastName":["Kieny"],"initials":["MP"],"affiliation":["Health Systems and Innovation Cluster, World Health Organization, Geneva, Switzerland ."]}]}],"journalInfo":[{"issue":["3"],"volume":["94"],"journalIssueId":["2385453"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["158"],"affiliation":["Department of Strategy, Policy & Information, World Health Organization, avenue Appia 20, Geneva 27, Switzerland ."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4773943?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4773943"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.170860"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-11"],"dateOfRevision":["2016-03-13"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4773943"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq76J9hGurOzZIY-","_score":1,"_source":{"id":["26938618"],"source":["MED"],"pmid":["26938618"],"pmcid":["PMC4777550"],"doi":["10.1371/journal.pntd.0004499"],"title":["Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients."],"authorString":["Petitdemange C, Wauquier N, Devilliers H, Yssel H, Mombo I, Caron M, Nkoghé D, Debré P, Leroy E, Vieillard V."],"authorList":[{"author":[{"fullName":["Petitdemange C"],"firstName":["Caroline"],"lastName":["Petitdemange"],"initials":["C"],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."]},{"fullName":["Wauquier N"],"firstName":["Nadia"],"lastName":["Wauquier"],"initials":["N"],"affiliation":["Metabiota, Inc., San Francisco, California, United States of America."]},{"fullName":["Devilliers H"],"firstName":["Hervé"],"lastName":["Devilliers"],"initials":["H"],"affiliation":["Internal Medicine and Systemic Diseases Department, Dijon University Hospital, Dijon, France."]},{"fullName":["Yssel H"],"firstName":["Hans"],"lastName":["Yssel"],"initials":["H"],"affiliation":["Sorbonne Universités, UPMC Univ Paris 06, INSERM U1135, CNRS ERL8255, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France."]},{"fullName":["Mombo I"],"firstName":["Illich"],"lastName":["Mombo"],"initials":["I"],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."]},{"fullName":["Caron M"],"firstName":["Mélanie"],"lastName":["Caron"],"initials":["M"],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."]},{"fullName":["Nkoghé D"],"firstName":["Dieudonné"],"lastName":["Nkoghé"],"initials":["D"],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."]},{"fullName":["Debré P"],"firstName":["Patrice"],"lastName":["Debré"],"initials":["P"],"affiliation":["Sorbonne Universités, UPMC Univ Paris 06, INSERM U1135, CNRS ERL8255, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France."]},{"fullName":["Leroy E"],"firstName":["Eric"],"lastName":["Leroy"],"initials":["E"],"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}}],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."]},{"fullName":["Vieillard V"],"firstName":["Vincent"],"lastName":["Vieillard"],"initials":["V"],"affiliation":["Sorbonne Universités, UPMC Univ Paris 06, INSERM U1135, CNRS ERL8255, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004499"],"abstractText":["Dengue virus (DENV) is the most prominent arbovirus worldwide, causing major epidemics in South-East Asia, South America and Africa. In 2010, a major DENV-2 outbreak occurred in Gabon with cases of patients co-infected with chikungunya virus (CHIKV). Although the innate immune response is thought to be of primordial importance in the development and outcome of arbovirus-associated pathologies, our knowledge of the role of natural killer (NK) cells during DENV-2 infection is in its infancy.We performed the first extensive comparative longitudinal characterization of NK cells in patients infected by DENV-2, CHIKV or both viruses. Hierarchical clustering and principal component analyses were performed to discriminate between CHIKV and DENV-2 infected patients.We observed that both activation and differentiation of NK cells are induced during the acute phase of infection by DENV-2 and CHIKV. Combinatorial analysis however, revealed that both arboviruses induced two different signatures of NK-cell responses, with CHIKV more associated with terminal differentiation, and DENV-2 with inhibitory KIRs. We show also that intracellular production of interferon-γ (IFN-γ) by NK cells is strongly stimulated in acute DENV-2 infection, compared to CHIKV.Although specific differences were observed between CHIKV and DENV-2 infections, the significant remodeling of NK cell populations observed here suggests their potential roles in the control of both infections."],"affiliation":["Centre International de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Killer Cells, Natural"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Longitudinal Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Gabon"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Coinfection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777550?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777550"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004499"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-03-04"],"dateOfRevision":["2016-03-11"],"electronicPublicationDate":["2016-03-03"],"firstPublicationDate":["2016-03-03"],"cprojectID":"PMC4777550"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq6-hOUS2orL1IPI","_score":1,"_source":{"id":["26909342"],"source":["MED"],"pmid":["26909342"],"pmcid":["PMC4754388"],"doi":["10.3389/fpubh.2016.00008"],"title":["Flaviviruses as a Cause of Undifferentiated Fever in Sindh Province, Pakistan: A Preliminary Report."],"authorString":["Khan E, Farooqi JQ, Barr KL, Prakoso D, Nasir A, Kanji A, Shakoor S, Malik FR, Hasan R, Lednicky JA, Long MT."],"authorList":[{"author":[{"fullName":["Khan E"],"firstName":["Erum"],"lastName":["Khan"],"initials":["E"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Farooqi JQ"],"firstName":["Joveria Q"],"lastName":["Farooqi"],"initials":["JQ"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Barr KL"],"firstName":["Kelli L"],"lastName":["Barr"],"initials":["KL"],"affiliation":["Emerging Diseases and Arbopathogens Research and Testing Laboratory, Department of Infectious Diseases and Pathology, University of Florida , Gainesville, FL , USA."]},{"fullName":["Prakoso D"],"firstName":["Dhani"],"lastName":["Prakoso"],"initials":["D"],"affiliation":["Department of Environmental and Global Health, Emerging Pathogens Institute, University of Florida , Gainesville, FL , USA."]},{"fullName":["Nasir A"],"firstName":["Amna"],"lastName":["Nasir"],"initials":["A"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Kanji A"],"firstName":["Akbar"],"lastName":["Kanji"],"initials":["A"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Shakoor S"],"firstName":["Sadia"],"lastName":["Shakoor"],"initials":["S"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Malik FR"],"firstName":["Faisal Riaz"],"lastName":["Malik"],"initials":["FR"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Hasan R"],"firstName":["Rumina"],"lastName":["Hasan"],"initials":["R"],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."]},{"fullName":["Lednicky JA"],"firstName":["John A"],"lastName":["Lednicky"],"initials":["JA"],"affiliation":["Department of Environmental and Global Health, Emerging Pathogens Institute, University of Florida , Gainesville, FL , USA."]},{"fullName":["Long MT"],"firstName":["Maureen T"],"lastName":["Long"],"initials":["MT"],"affiliation":["Emerging Diseases and Arbopathogens Research and Testing Laboratory, Department of Infectious Diseases and Pathology, University of Florida , Gainesville, FL , USA."]}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2374588"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in public health"],"ISOAbbreviation":["Front Public Health"],"medlineAbbreviation":["Front Public Health"],"NLMid":["101616579"],"ESSN":["2296-2565"]}]}],"pubYear":["2016"],"pageInfo":["8"],"abstractText":["Arboviral diseases are expanding worldwide, yet global surveillance is often limited due to diplomatic and cultural barriers between nations. With human encroachment into new habitats, mosquito-borne viruses are also invading new areas. The actual prevalence of expanding arboviruses is unknown in Pakistan due to inappropriate diagnosis and poor testing for arboviral diseases. The primary objective of this study was to document evidence of flavivirus infections as the cause of undifferentiated fever in Pakistan. Through a cooperative effort between the USA and Pakistan, patient exposure to dengue virus (DENV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) was examined in Sindh Province for the first time in decades. Initial results from the 2015 arbovirus season consisting of a cross-sectional study of 467 patients in 5 sites, DENV NS1 antigen was identified in 63 of the screened subjects, WNV IgM antibodies in 16 patients, and JEV IgM antibodies in 32 patients. In addition, a number of practical findings were made including (1) in silico optimization of RT-PCR primers for flavivirus strains circulating in the Middle East, (2) shipping and storage of RT-PCR master mix and other reagents at ambient temperature, (3) Smart phone applications for the collection of data in areas with limited infrastructure, and (4) fast and reliable shipping for transport of reagents and specimens to and from the Middle East. Furthermore, this work is producing a group of highly trained local scientists and medical professionals disseminating modern scientific methods and more accurate diagnostic procedures to the community."],"affiliation":["Department of Pathology, Aga Khan University, Karachi, Pakistan; Department of Microbiology, Aga Khan University, Karachi, Pakistan."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Japanese encephalitis virus","West Nile virus","Arbovirus","Flavivirus","Dengue Viruses","Transboundary Diseases"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4754388?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4754388"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fpubh.2016.00008"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-02-24"],"dateOfCreation":["2016-02-24"],"dateOfRevision":["2016-02-28"],"electronicPublicationDate":["2016-02-16"],"firstPublicationDate":["2016-02-16"],"cprojectID":"PMC4754388"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrHdhOUS2orL1IPg","_score":1,"_source":{"id":["27004142"],"source":["MED"],"pmid":["27004142"],"pmcid":["PMC4798858"],"doi":["10.15844/pedneurbriefs-30-1-7"],"title":["Zika Virus Infection and Microcephaly."],"authorString":["Millichap JG."],"authorList":[{"author":[{"fullName":["Millichap JG"],"firstName":["J Gordon"],"lastName":["Millichap"],"initials":["JG"],"authorId":[{"_":"0000-0002-0173-7931","$":{"type":"ORCID"}}],"affiliation":["Division of Neurology, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL; Departments of Pediatrics and Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0173-7931","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["30"],"journalIssueId":["2389705"],"dateOfPublication":["2016 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Pediatric neurology briefs"],"ISOAbbreviation":["Pediatr Neurol Briefs"],"medlineAbbreviation":["Pediatr Neurol Briefs"],"NLMid":["9889575"],"ISSN":["1043-3155"],"ESSN":["2166-6482"]}]}],"pubYear":["2016"],"pageInfo":["8"],"abstractText":["A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy."],"affiliation":["Division of Neurology, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL; Departments of Pediatrics and Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Brazil","Microcephaly","Aedes Mosquitoes","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4798858?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4798858"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.15844/pedneurbriefs-30-1-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-03-23"],"dateOfCreation":["2016-03-23"],"dateOfRevision":["2016-03-25"],"firstPublicationDate":["2016-01-01"],"cprojectID":"PMC4798858"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrH2hOUS2orL1IPj","_score":1,"_source":{"id":["27007411"],"source":["MED"],"pmid":["27007411"],"pmcid":["PMC4805253"],"doi":["10.1371/journal.pone.0151403"],"title":["Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations."],"authorString":["Souza RS, Diaz-Albiter HM, Dillon VM, Dillon RJ, Genta FA."],"authorList":[{"author":[{"fullName":["Souza RS"],"firstName":["Raquel Santos"],"lastName":["Souza"],"initials":["RS"],"affiliation":["Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av, Leonidas Deane Building, room 207, Manguinhos, Rio de Janeiro, Brazil, 21040-360."]},{"fullName":["Diaz-Albiter HM"],"firstName":["Hector Manuel"],"lastName":["Diaz-Albiter"],"initials":["HM"],"affiliation":["Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av, Leonidas Deane Building, room 207, Manguinhos, Rio de Janeiro, Brazil, 21040-360."]},{"fullName":["Dillon VM"],"firstName":["Vivian Maureen"],"lastName":["Dillon"],"initials":["VM"],"affiliation":["Institute of Integrative Biology, Biosciences Building, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom."]},{"fullName":["Dillon RJ"],"firstName":["Rod J"],"lastName":["Dillon"],"initials":["RJ"],"affiliation":["National Institute of Science and Technology for Molecular Entomology, 373 Carlos Chagas Filho Av., Center for Health Science, Building D, Basement, room 5, Cidade Universitária, Rio de Janeiro, Brazil, 21941-590."]},{"fullName":["Genta FA"],"firstName":["Fernando Ariel"],"lastName":["Genta"],"initials":["FA"],"affiliation":["National Institute of Science and Technology for Molecular Entomology, 373 Carlos Chagas Filho Av., Center for Health Science, Building D, Basement, room 5, Cidade Universitária, Rio de Janeiro, Brazil, 21941-590."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3551-0030","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["11"],"journalIssueId":["2380071"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0151403"],"abstractText":["Aedes aegypti larvae ingest several kinds of microorganisms. In spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. We investigated the effects of using yeasts as the sole nutrient source for A. aegypti larvae. We also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. Beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. Larvae were fed with cat food (controls), live or autoclaved Saccharomyces cerevisiae cells and larval weight, time for pupation and adult emergence, larval and pupal mortality were measured. The presence of S. cerevisiae cells inside the larval gut was demonstrated by light microscopy. Beta-1,3-glucanase was measured in dissected larval samples. Viability assays were performed with live yeast cells and larval gut homogenates, with or without addition of competing beta-1,3-glucan. A. aegypti larvae fed with yeast cells were heavier at the 4th instar and showed complete development with normal mortality rates. Yeast cells were efficiently ingested by larvae and quickly killed (10% death in 2 h, 100% in 48 h). Larvae showed beta-1,3-glucanase in head, gut and rest of body. Gut beta-1,3-glucanase was not derived from ingested yeast cells. Gut and rest of body activity was not affected by the yeast diet, but head homogenates showed a lower activity in animals fed with autoclaved S. cerevisiae cells. The enzymatic lysis of live S. cerevisiae cells was demonstrated using gut homogenates, and this activity was abolished when excess beta-1,3-glucan was added to assays. These results show that live yeast cells are efficiently ingested and hydrolyzed by A. aegypti larvae, which are able to fully-develop on a diet based exclusively on these organisms. Beta-1,3-glucanase seems to be essential for yeast lytic activity of A. aegypti larvae, which possess significant amounts of these enzyme in all parts investigated."],"affiliation":["Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av, Leonidas Deane Building, room 207, Manguinhos, Rio de Janeiro, Brazil, 21040-360."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Saccharomyces cerevisiae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EN"],"qualifierName":["enzymology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Glucan 1,3-beta-Glucosidase"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Larva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EN"],"qualifierName":["enzymology"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["beta-1,3-exoglucanase"],"registryNumber":["EC 3.2.1.58"]},{"name":["Glucan 1,3-beta-Glucosidase"],"registryNumber":["EC 3.2.1.58"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4805253?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4805253"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0151403"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-01"],"dateOfCreation":["2016-03-24"],"dateOfRevision":["2016-03-26"],"electronicPublicationDate":["2016-03-23"],"firstPublicationDate":["2016-03-23"],"cprojectID":"PMC4805253"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrIZJ9hGurOzZIZd","_score":1,"_source":{"id":["27148186"],"source":["MED"],"pmid":["27148186"],"pmcid":["PMC4835484"],"doi":["10.3389/fmicb.2016.00496"],"title":["Zika Virus: the Latest Newcomer."],"authorString":["Saiz JC, Vázquez-Calvo Á, Blázquez AB, Merino-Ramos T, Escribano-Romero E, Martín-Acebes MA."],"authorList":[{"author":[{"fullName":["Saiz JC"],"firstName":["Juan-Carlos"],"lastName":["Saiz"],"initials":["JC"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Vázquez-Calvo Á"],"firstName":["Ángela"],"lastName":["Vázquez-Calvo"],"initials":["Á"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Blázquez AB"],"firstName":["Ana B"],"lastName":["Blázquez"],"initials":["AB"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Merino-Ramos T"],"firstName":["Teresa"],"lastName":["Merino-Ramos"],"initials":["T"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Escribano-Romero E"],"firstName":["Estela"],"lastName":["Escribano-Romero"],"initials":["E"],"authorId":[{"_":"0000-0002-5036-7607","$":{"type":"ORCID"}}],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Martín-Acebes MA"],"firstName":["Miguel A"],"lastName":["Martín-Acebes"],"initials":["MA"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5036-7607","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2369092"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2016"],"pageInfo":["496"],"abstractText":["Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980's, then in the Micronesia in 2007 and, more recently in the Americas in 2014, where it has displayed an explosive spread, as advised by the World Health Organization, which resulted in the infection of hundreds of thousands of people. ZIKV infection was characterized by causing a mild disease presented with fever, headache, rash, arthralgia, and conjunctivitis, with exceptional reports of an association with Guillain-Barre syndrome (GBS) and microcephaly. However, since the end of 2015, an increase in the number of GBS associated cases and an astonishing number of microcephaly in fetus and new-borns in Brazil have been related to ZIKV infection, raising serious worldwide public health concerns. Clarifying such worrisome relationships is, thus, a current unavoidable goal. Here, we extensively review what is currently known about ZIKV, from molecular biology, transmission routes, ecology, and epidemiology, to clinical manifestations, pathogenesis, diagnosis, prophylaxis, and public health."],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Zoonosis","outbreak","Microcephaly","Flavivirus","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4835484?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4835484"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2016.00496"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27443350"],"source":["MED"],"reference":["Front Microbiol. 2016;7:1028"],"type":["Comment in"],"orderIn":["1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["refseq"]}],"dateOfCompletion":["2016-05-05"],"dateOfCreation":["2016-05-05"],"dateOfRevision":["2016-07-22"],"electronicPublicationDate":["2016-04-19"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4835484"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrJkhOUS2orL1IPq","_score":1,"_source":{"id":["27069825"],"source":["MED"],"pmid":["27069825"],"pmcid":["PMC4824915"],"doi":["10.7717/peerj.1904"],"title":["Estimating risks of importation and local transmission of Zika virus infection."],"authorString":["Nah K, Mizumoto K, Miyamatsu Y, Yasuda Y, Kinoshita R, Nishiura H."],"authorList":[{"author":[{"fullName":["Nah K"],"firstName":["Kyeongah"],"lastName":["Nah"],"initials":["K"],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan."]},{"fullName":["Mizumoto K"],"firstName":["Kenji"],"lastName":["Mizumoto"],"initials":["K"],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan; Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan."]},{"fullName":["Miyamatsu Y"],"firstName":["Yuichiro"],"lastName":["Miyamatsu"],"initials":["Y"],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan."]},{"fullName":["Yasuda Y"],"firstName":["Yohei"],"lastName":["Yasuda"],"initials":["Y"],"affiliation":["Graduate School of Medicine, The University of Tokyo , Tokyo , Japan."]},{"fullName":["Kinoshita R"],"firstName":["Ryo"],"lastName":["Kinoshita"],"initials":["R"],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan."]},{"fullName":["Nishiura H"],"firstName":["Hiroshi"],"lastName":["Nishiura"],"initials":["H"],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7708-1019","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2370428"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PeerJ"],"ISOAbbreviation":["PeerJ"],"medlineAbbreviation":["PeerJ"],"NLMid":["101603425"],"ESSN":["2167-8359"]}]}],"pubYear":["2016"],"pageInfo":["e1904"],"abstractText":["Background. An international spread of Zika virus (ZIKV) infection has attracted global attention. ZIKV is conveyed by a mosquito vector, Aedes species, which also acts as the vector species of dengue and chikungunya viruses. Methods. Arrival time of ZIKV importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. Employing a survival analysis model in which the hazard is an inverse function of the effective distance as informed by the airline transportation network data, and using dengue and chikungunya virus transmission data, risks of importation and local transmission were estimated. Results. A total of 78 countries with imported case(s) have been identified, with the arrival time ranging from 1 to 44 weeks since the first ZIKV was identified in Brazil, 2015. Whereas the risk of importation was well explained by the airline transportation network data, the risk of local transmission appeared to be best captured by additionally accounting for the presence of dengue and chikungunya viruses. Discussion. The risk of importation may be high given continued global travel of mildly infected travelers but, considering that the public health concerns over ZIKV infection stems from microcephaly, it is more important to focus on the risk of local and widespread transmission that could involve pregnant women. The predicted risk of local transmission was frequently seen in tropical and subtropical countries with dengue or chikungunya epidemic experience."],"affiliation":["Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; CREST, Japan Science and Technology Agency, Saitama, Japan; Graduate School of Medicine, Hokkaido University, Sapporo, Japan."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Network","Transmission","epidemiology","mathematical model","risk","Importation","Statistical Estimation","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4824915?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4824915"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7717/peerj.1904"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-12"],"dateOfCreation":["2016-04-12"],"dateOfRevision":["2016-04-15"],"electronicPublicationDate":["2016-04-05"],"firstPublicationDate":["2016-04-05"],"cprojectID":"PMC4824915"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrOPJ9hGurOzZIZr","_score":1,"_source":{"id":["27129407"],"source":["MED"],"pmid":["27129407"],"pmcid":["PMC4850678"],"doi":["10.1186/s12879-016-1517-z"],"title":["The risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in Rio de Janeiro, Brazil."],"authorString":["Ximenes R, Amaku M, Lopez LF, Coutinho FA, Burattini MN, Greenhalgh D, Wilder-Smith A, Struchiner CJ, Massad E."],"authorList":[{"author":[{"fullName":["Ximenes R"],"firstName":["Raphael"],"lastName":["Ximenes"],"initials":["R"],"authorId":[{"_":"0000-0003-2536-951X","$":{"type":"ORCID"}}],"affiliation":["School of Medicine, University of São Paulo and LIM01-HCFMUSP, São Paulo, SP, Brazil."]},{"fullName":["Amaku M"],"firstName":["Marcos"],"lastName":["Amaku"],"initials":["M"],"affiliation":["School of Medicine, University of São Paulo and LIM01-HCFMUSP, São Paulo, SP, Brazil."]},{"fullName":["Lopez LF"],"firstName":["Luis Fernandez"],"lastName":["Lopez"],"initials":["LF"],"affiliation":["Center for Internet Augmented Research &Assessment, Florida International University, Miami, FL, USA."]},{"fullName":["Coutinho FA"],"firstName":["Francisco Antonio Bezerra"],"lastName":["Coutinho"],"initials":["FA"],"affiliation":["School of Medicine, University of São Paulo and LIM01-HCFMUSP, São Paulo, SP, Brazil."]},{"fullName":["Burattini MN"],"firstName":["Marcelo Nascimento"],"lastName":["Burattini"],"initials":["MN"],"affiliation":["Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil."]},{"fullName":["Greenhalgh D"],"firstName":["David"],"lastName":["Greenhalgh"],"initials":["D"],"affiliation":["University of Strathclyde, Glasgow, UK."]},{"fullName":["Wilder-Smith A"],"firstName":["Annelies"],"lastName":["Wilder-Smith"],"initials":["A"],"affiliation":["Lee Kong Chian School of Medicine, Nanyang University, Singapore, Singapore."]},{"fullName":["Struchiner CJ"],"firstName":["Claudio José"],"lastName":["Struchiner"],"initials":["CJ"],"affiliation":["Programme of Scientific Computation, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Massad E"],"firstName":["Eduardo"],"lastName":["Massad"],"initials":["E"],"affiliation":["London School of Hygiene and Tropical Medicine, London, UK. edmassad@usp.br."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2536-951X","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["16"],"journalIssueId":["2401698"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC infectious diseases"],"ISOAbbreviation":["BMC Infect. Dis."],"medlineAbbreviation":["BMC Infect Dis"],"NLMid":["100968551"],"ESSN":["1471-2334"]}]}],"pubYear":["2016"],"pageInfo":["186"],"abstractText":["Rio de Janeiro in Brazil will host the Summer Olympic Games in 2016. About 400,000 non-immune foreign tourists are expected to attend the games. As Brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified.A mathematical model to calculate the risk of developing dengue for foreign tourists attending the Olympic Games in Rio de Janeiro in 2016 is proposed. A system of differential equation models the spread of dengue amongst the resident population and a stochastic approximation is used to assess the risk to tourists. Historical reported dengue time series in Rio de Janeiro for the years 2000-2015 is used to find out the time dependent force of infection, which is then used to estimate the potential risks to a large tourist cohort. The worst outbreak of dengue occurred in 2012 and this and the other years in the history of Dengue in Rio are used to discuss potential risks to tourists amongst visitors to the forthcoming Rio Olympics.The individual risk to be infected by dengue is very much dependent on the ratio asymptomatic/symptomatic considered but independently of this the worst month of August in the period studied in terms of dengue transmission, occurred in 2007.If dengue returns in 2016 with the pattern observed in the worst month of August in history (2007), the expected number of symptomatic and asymptomatic dengue cases among tourists will be 23 and 206 cases, respectively. This worst case scenario would have an incidence of 5.75 (symptomatic) and 51.5 (asymptomatic) per 100,000 individuals."],"affiliation":["School of Medicine, University of São Paulo and LIM01-HCFMUSP, São Paulo, SP, Brazil."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Mathematical models","Olympic Games","Dengue","risk assessment","Travel Medicine"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4850678?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4850678"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12879-016-1517-z"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-30"],"dateOfRevision":["2016-05-02"],"electronicPublicationDate":["2016-04-29"],"firstPublicationDate":["2016-04-29"],"cprojectID":"PMC4850678"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrO0J9hGurOzZIZt","_score":1,"_source":{"id":["27135922"],"source":["MED"],"pmid":["27135922"],"pmcid":["PMC4852896"],"doi":["10.1371/journal.pntd.0004676"],"title":["Potential Impact of Sexual Transmission on Ebola Virus Epidemiology: Sierra Leone as a Case Study."],"authorString":["Abbate JL, Murall CL, Richner H, Althaus CL."],"authorList":[{"author":[{"fullName":["Abbate JL"],"firstName":["Jessica L"],"lastName":["Abbate"],"initials":["JL"],"authorId":[{"_":"0000-0003-2485-3334","$":{"type":"ORCID"}}],"affiliation":["UMR UMMISCO (UMI 209 IRD-UPMC), Bondy, France."]},{"fullName":["Murall CL"],"firstName":["Carmen Lia"],"lastName":["Murall"],"initials":["CL"],"affiliation":["Max-Planck Institute for Dynamics and Self-Organization, Gottingen, Germany."]},{"fullName":["Richner H"],"firstName":["Heinz"],"lastName":["Richner"],"initials":["H"],"affiliation":["Institute for Ecology and Evolution, University of Bern, Bern, Switzerland."]},{"fullName":["Althaus CL"],"firstName":["Christian L"],"lastName":["Althaus"],"initials":["CL"],"affiliation":["Institute of Social and Preventive Medicine (ISPM), University of Bern, Bern, Switzerland."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2485-3334","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004676"],"abstractText":["Sexual transmission of Ebola virus disease (EVD) 6 months after onset of symptoms has been recently documented, and Ebola virus RNA has been detected in semen of survivors up to 9 months after onset of symptoms. As countries affected by the 2013-2015 epidemic in West Africa, by far the largest to date, are declared free of Ebola virus disease (EVD), it remains unclear what threat is posed by rare sexual transmission events that could arise from survivors.We devised a compartmental mathematical model that includes sexual transmission from convalescent survivors: a SEICR (susceptible-exposed-infectious-convalescent-recovered) transmission model. We fitted the model to weekly incidence of EVD cases from the 2014-2015 epidemic in Sierra Leone. Sensitivity analyses and Monte Carlo simulations showed that a 0.1% per sex act transmission probability and a 3-month convalescent period (the two key unknown parameters of sexual transmission) create very few additional cases, but would extend the epidemic by 83 days [95% CI: 68-98 days] (p < 0.0001) on average. Strikingly, a 6-month convalescent period extended the average epidemic by 540 days (95% CI: 508-572 days), doubling the current length, despite an insignificant rise in the number of new cases generated.Our results show that reductions in the per sex act transmission probability via abstinence and condom use should reduce the number of sporadic sexual transmission events, but will not significantly reduce the epidemic size and may only minimally shorten the length of time the public health community must maintain response preparedness. While the number of infectious survivors is expected to greatly decline over the coming months, our results show that transmission events may still be expected for quite some time as each event results in a new potential cluster of non-sexual transmission. Precise measurement of the convalescent period is thus important for planning ongoing surveillance efforts."],"affiliation":["UMR UMMISCO (UMI 209 IRD-UPMC), Bondy, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4852896?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4852896"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004676"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-04"],"dateOfRevision":["2016-05-14"],"electronicPublicationDate":["2016-05-02"],"firstPublicationDate":["2016-05-02"],"cprojectID":"PMC4852896"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrPqhOUS2orL1IP8","_score":1,"_source":{"id":["27111294"],"source":["MED"],"pmid":["27111294"],"pmcid":["PMC4844159"],"doi":["10.1371/journal.pntd.0004687"],"title":["Zika Virus Outbreak in Haiti in 2014: Molecular and Clinical Data."],"authorString":["Lednicky J, Beau De Rochars VM, El Badry M, Loeb J, Telisma T, Chavannes S, Anilis G, Cella E, Ciccozzi M, Rashid M, Okech B, Salemi M, Morris JG."],"authorList":[{"author":[{"fullName":["Lednicky J"],"firstName":["John"],"lastName":["Lednicky"],"initials":["J"],"affiliation":["Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Beau De Rochars VM"],"firstName":["Valery Madsen"],"lastName":["Beau De Rochars"],"initials":["VM"],"affiliation":["Department of Health Services Research, Management, and Policy, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["El Badry M"],"firstName":["Maha"],"lastName":["El Badry"],"initials":["M"],"affiliation":["Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Loeb J"],"firstName":["Julia"],"lastName":["Loeb"],"initials":["J"],"affiliation":["Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Telisma T"],"firstName":["Taina"],"lastName":["Telisma"],"initials":["T"],"affiliation":["Christianville Foundation School Clinic, Gressier, Haiti."]},{"fullName":["Chavannes S"],"firstName":["Sonese"],"lastName":["Chavannes"],"initials":["S"],"affiliation":["Christianville Foundation School Clinic, Gressier, Haiti."]},{"fullName":["Anilis G"],"firstName":["Gina"],"lastName":["Anilis"],"initials":["G"],"affiliation":["Christianville Foundation School Clinic, Gressier, Haiti."]},{"fullName":["Cella E"],"firstName":["Eleonora"],"lastName":["Cella"],"initials":["E"],"authorId":[{"_":"0000-0002-7870-9744","$":{"type":"ORCID"}}],"affiliation":["Department of Infectious Parasitic and Immunomediated Diseases, Reference Centre on Phylogeny, Molecular Epidemiology and Microbial Evolution (FEMEM)/Epidemiology Unit, Istituto Superiore di Sanita, Rome, Italy."]},{"fullName":["Ciccozzi M"],"firstName":["Massimo"],"lastName":["Ciccozzi"],"initials":["M"],"authorId":[{"_":"0000-0003-3866-9239","$":{"type":"ORCID"}}],"affiliation":["Department of Infectious Parasitic and Immunomediated Diseases, Reference Centre on Phylogeny, Molecular Epidemiology and Microbial Evolution (FEMEM)/Epidemiology Unit, Istituto Superiore di Sanita, Rome, Italy."]},{"fullName":["Rashid M"],"firstName":["Mohammed"],"lastName":["Rashid"],"initials":["M"],"affiliation":["Emerging Pathogens Institute, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Okech B"],"firstName":["Bernard"],"lastName":["Okech"],"initials":["B"],"affiliation":["Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Salemi M"],"firstName":["Marco"],"lastName":["Salemi"],"initials":["M"],"affiliation":["Department of Pathology, Immunology, and Laboratory Sciences, College of Medicine, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Morris JG Jr"],"firstName":["J Glenn"],"lastName":["Morris"],"initials":["JG"],"affiliation":["Department of Medicine, College of Medicine, University of Florida, Gainesville, Florida, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7870-9744","$":{"type":"ORCID"}},{"_":"0000-0003-3866-9239","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004687"],"abstractText":["Zika virus (ZIKV), first isolated in Uganda in 1947, is currently spreading rapidly through South America and the Caribbean. In Brazil, infection has been linked with microcephaly and other serious complications, leading to declaration of a public health emergency of international concern; however, there currently are only limited data on the virus (and its possible sources and manifestations) in the Caribbean.From May, 2014-February, 2015, in conjunction with studies of chikungunya (CHIKV) and dengue (DENV) virus infections, blood samples were collected from children in the Gressier/Leogane region of Haiti who presented to a school clinic with undifferentiated febrile illness. Samples were initially screened by RT-PCR for CHIKV and DENV, with samples negative in these assays further screened by viral culture.Of 177 samples screened, three were positive for ZIKV, confirmed by viral sequencing; DENV-1 was also identified in culture from one of the three positive case patients. Patients were from two different schools and 3 different towns, with all three cases occurring within a single week, consistent with the occurrence of an outbreak in the region. Phylogenetic analysis of known full genome viral sequences demonstrated a close relationship with ZIKV from Brazil; additional analysis of the NS5 gene, for which more sequences are currently available, showed the Haitian strains clustering within a monophyletic clade distinct from Brazilian, Puerto Rican and Guatemalan sequences, with all part of a larger clade including isolates from Easter Island. Phylogeography also clarified that at least three major African sub-lineages exist, and confirmed that the South American epidemic is most likely to have originated from an initial ZIKV introduction from French Polynesia into Easter Island, and then to the remainder of the Americas.ZIKV epidemics in South America, as well as in Africa, show complex dissemination patterns. The virus appears to have been circulating in Haiti prior to the first reported cases in Brazil. Factors contributing to transmission and the possible linkage of this early Haitian outbreak with microcephaly remain to be determined."],"affiliation":["Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Viral Nonstructural Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Virus Cultivation"]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Homology"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Child"]},{"majorTopic_YN":["N"],"descriptorName":["Child, Preschool"]},{"majorTopic_YN":["N"],"descriptorName":["Infant"]},{"majorTopic_YN":["N"],"descriptorName":["Haiti"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeography"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["NS5 protein, flavivirus"],"registryNumber":["0"]},{"name":["RNA, Viral"],"registryNumber":["0"]},{"name":["Viral Nonstructural Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4844159?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4844159"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004687"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-26"],"dateOfRevision":["2016-05-06"],"electronicPublicationDate":["2016-04-25"],"firstPublicationDate":["2016-04-25"],"cprojectID":"PMC4844159"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrQH_8VqgcuJwvhv","_score":1,"_source":{"id":["27068686"],"source":["MED"],"pmid":["27068686"],"pmcid":["PMC4828820"],"doi":["10.1186/s12936-016-1252-3"],"title":["Key traveller groups of relevance to spatial malaria transmission: a survey of movement patterns in four sub-Saharan African countries."],"authorString":["Marshall JM, Touré M, Ouédraogo AL, Ndhlovu M, Kiware SS, Rezai A, Nkhama E, Griffin JT, Hollingsworth TD, Doumbia S, Govella NJ, Ferguson NM, Ghani AC."],"authorList":[{"author":[{"fullName":["Marshall JM"],"firstName":["John M"],"lastName":["Marshall"],"initials":["JM"],"affiliation":["Divisions of Biostatistics and Epidemiology, School of Public Health, University of California, Berkeley, CA, USA. john.marshall@berkeley.edu."]},{"fullName":["Touré M"],"firstName":["Mahamoudou"],"lastName":["Touré"],"initials":["M"],"affiliation":["Malaria Research and Training Center, University of Bamako, Bamako, Mali."]},{"fullName":["Ouédraogo AL"],"firstName":["André Lin"],"lastName":["Ouédraogo"],"initials":["AL"],"affiliation":["Institute for Disease Modeling, Bellevue, WA, USA."]},{"fullName":["Ndhlovu M"],"firstName":["Micky"],"lastName":["Ndhlovu"],"initials":["M"],"affiliation":["Chainama College of Health Sciences, Lusaka, Zambia."]},{"fullName":["Kiware SS"],"firstName":["Samson S"],"lastName":["Kiware"],"initials":["SS"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Dar es Salaam, Tanzania."]},{"fullName":["Rezai A"],"firstName":["Ashley"],"lastName":["Rezai"],"initials":["A"],"affiliation":["Department of Infectious Disease Epidemiology, MRC Center for Outbreak Analysis and Modelling, Imperial College London, London, UK."]},{"fullName":["Nkhama E"],"firstName":["Emmy"],"lastName":["Nkhama"],"initials":["E"],"affiliation":["Chainama College of Health Sciences, Lusaka, Zambia."]},{"fullName":["Griffin JT"],"firstName":["Jamie T"],"lastName":["Griffin"],"initials":["JT"],"affiliation":["Department of Infectious Disease Epidemiology, MRC Center for Outbreak Analysis and Modelling, Imperial College London, London, UK."]},{"fullName":["Hollingsworth TD"],"firstName":["T Deirdre"],"lastName":["Hollingsworth"],"initials":["TD"],"affiliation":["School of Life Sciences, University of Warwick, Warwick, Coventry, UK."]},{"fullName":["Doumbia S"],"firstName":["Seydou"],"lastName":["Doumbia"],"initials":["S"],"affiliation":["Malaria Research and Training Center, University of Bamako, Bamako, Mali."]},{"fullName":["Govella NJ"],"firstName":["Nicodem J"],"lastName":["Govella"],"initials":["NJ"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Dar es Salaam, Tanzania."]},{"fullName":["Ferguson NM"],"firstName":["Neil M"],"lastName":["Ferguson"],"initials":["NM"],"affiliation":["Department of Infectious Disease Epidemiology, MRC Center for Outbreak Analysis and Modelling, Imperial College London, London, UK."]},{"fullName":["Ghani AC"],"firstName":["Azra C"],"lastName":["Ghani"],"initials":["AC"],"affiliation":["Department of Infectious Disease Epidemiology, MRC Center for Outbreak Analysis and Modelling, Imperial College London, London, UK."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2401987"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Malaria journal"],"ISOAbbreviation":["Malar. J."],"medlineAbbreviation":["Malar J"],"NLMid":["101139802"],"ESSN":["1475-2875"]}]}],"pubYear":["2016"],"pageInfo":["200"],"abstractText":["As malaria prevalence declines in many parts of the world due to widescale control efforts and as drug-resistant parasites begin to emerge, a quantitative understanding of human movement is becoming increasingly relevant to malaria control. However, despite its importance, significant knowledge gaps remain regarding human movement, particularly in sub-Saharan Africa.A quantitative survey of human movement patterns was conducted in four countries in sub-Saharan Africa: Mali, Burkina Faso, Zambia, and Tanzania, with three to five survey locations chosen in each country. Questions were included on demographic and trip details, malaria risk behaviour, children accompanying travellers, and mobile phone usage to enable phone signal data to be better correlated with movement. A total of 4352 individuals were interviewed and 6411 trips recorded.A cluster analysis of trips highlighted two distinct traveller groups of relevance to malaria transmission: women travelling with children (in all four countries) and youth workers (in Mali). Women travelling with children were more likely to travel to areas of relatively high malaria prevalence in Mali (OR = 4.46, 95% CI = 3.42-5.83), Burkina Faso (OR = 1.58, 95% CI = 1.23-1.58), Zambia (OR = 1.50, 95% CI = 1.20-1.89), and Tanzania (OR = 2.28, 95% CI = 1.71-3.05) compared to other travellers. They were also more likely to own bed nets in Burkina Faso (OR = 1.77, 95% CI = 1.25-2.53) and Zambia (OR = 1.74, 95% CI = 1.34 2.27), and less likely to own a mobile phone in Mali (OR = 0.50, 95% CI = 0.39-0.65), Burkina Faso (OR = 0.39, 95% CI = 0.30-0.52), and Zambia (OR = 0.60, 95% CI = 0.47-0.76). Malian youth workers were more likely to travel to areas of relatively high malaria prevalence (OR = 23, 95% CI = 17-31) and for longer durations (mean of 70 days cf 21 days, p < 0.001) compared to other travellers.Women travelling with children were a remarkably consistent traveller group across all four countries surveyed. They are expected to contribute greatly towards spatial malaria transmission because the children they travel with tend to have high parasite prevalence. Youth workers were a significant traveller group in Mali and are expected to contribute greatly to spatial malaria transmission because their movements correlate with seasonal rains and hence peak mosquito densities. Interventions aimed at interrupting spatial transmission of parasites should consider these traveller groups."],"affiliation":["Divisions of Biostatistics and Epidemiology, School of Public Health, University of California, Berkeley, CA, USA. john.marshall@berkeley.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["G1002284"],"agency":["Medical Research Council"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Cluster analysis","Plasmodium falciparum","Tanzania","Burkina Faso","Zambia","Mali","Mobile Phones","Youth Workers","Spatial Transmission","Women With Children"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4828820?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4828820"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12936-016-1252-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-12"],"dateOfRevision":["2016-05-31"],"electronicPublicationDate":["2016-04-12"],"firstPublicationDate":["2016-04-12"],"cprojectID":"PMC4828820"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrQmhOUS2orL1IP_","_score":1,"_source":{"id":["27508289"],"source":["MED"],"pmid":["27508289"],"pmcid":["PMC4852032"],"title":["WHO AND EXPERTS PRIORITIZE VACCINES, DIAGNOSTICS AND INNOVATIVE VECTOR CONTROL TOOLS FOR ZIKA R&D."],"journalInfo":[{"issue":["4"],"volume":["37"],"journalIssueId":["2395226"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Saudi medical journal"],"ISOAbbreviation":["Saudi Med J"],"medlineAbbreviation":["Saudi Med J"],"NLMid":["7909441"],"ISSN":["0379-5284"]}]}],"pubYear":["2016"],"pageInfo":["471-472"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4852032?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4852032"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-09"],"dateOfRevision":["2016-08-14"],"firstPublicationDate":["2016-04-01"],"cprojectID":"PMC4852032"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRb_8VqgcuJwvh1","_score":1,"_source":{"id":["26937036"],"source":["MED"],"pmid":["26937036"],"pmcid":["PMC4836334"],"doi":["10.1128/jvi.02586-15"],"title":["MicroRNA-19b-3p Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF11."],"authorString":["Ashraf U, Zhu B, Ye J, Wan S, Nie Y, Chen Z, Cui M, Wang C, Duan X, Zhang H, Chen H, Cao S."],"authorList":[{"author":[{"fullName":["Ashraf U"],"firstName":["Usama"],"lastName":["Ashraf"],"initials":["U"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Zhu B"],"firstName":["Bibo"],"lastName":["Zhu"],"initials":["B"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Ye J"],"firstName":["Jing"],"lastName":["Ye"],"initials":["J"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Wan S"],"firstName":["Shengfeng"],"lastName":["Wan"],"initials":["S"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Nie Y"],"firstName":["Yanru"],"lastName":["Nie"],"initials":["Y"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Chen Z"],"firstName":["Zheng"],"lastName":["Chen"],"initials":["Z"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Cui M"],"firstName":["Min"],"lastName":["Cui"],"initials":["M"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Wang C"],"firstName":["Chong"],"lastName":["Wang"],"initials":["C"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Duan X"],"firstName":["Xiaodong"],"lastName":["Duan"],"initials":["X"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Zhang H"],"firstName":["Hao"],"lastName":["Zhang"],"initials":["H"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Chen H"],"firstName":["Huanchun"],"lastName":["Chen"],"initials":["H"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."]},{"fullName":["Cao S"],"firstName":["Shengbo"],"lastName":["Cao"],"initials":["S"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China sbcao@mail.hzau.edu.cn."]}]}],"journalInfo":[{"issue":["9"],"volume":["90"],"journalIssueId":["2398778"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Journal of virology"],"ISOAbbreviation":["J. Virol."],"medlineAbbreviation":["J Virol"],"NLMid":["0113724"],"ISSN":["0022-538X"],"ESSN":["1098-5514"]}]}],"pubYear":["2016"],"pageInfo":["4780-4795"],"abstractText":["Japanese encephalitis virus (JEV) can invade the central nervous system and consequently induce neuroinflammation, which is characterized by profound neuronal cell damage accompanied by astrogliosis and microgliosis. Albeit microRNAs (miRNAs) have emerged as major regulatory noncoding RNAs with profound effects on inflammatory response, it is unknown how astrocytic miRNAs regulate JEV-induced inflammation. Here, we found the involvement of miR-19b-3p in regulating the JEV-induced inflammatory responsein vitroandin vivo The data demonstrated that miR-19b-3p is upregulated in cultured cells and mouse brain tissues during JEV infection. Overexpression of miR-19b-3p led to increased production of inflammatory cytokines, including tumor necrosis factor alpha, interleukin-6, interleukin-1β, and chemokine (C-C motif) ligand 5, after JEV infection, whereas knockdown of miR-19b-3p had completely opposite effects. Mechanistically, miR-19b-3p modulated the JEV-induced inflammatory response via targeting ring finger protein 11, a negative regulator of nuclear factor kappa B signaling. We also found that inhibition of ring finger protein 11 by miR-19b-3p resulted in accumulation of nuclear factor kappa B in the nucleus, which in turn led to higher production of inflammatory cytokines.In vivosilencing of miR-19b-3p by a specific antagomir reinvigorates the expression level of RNF11, which in turn reduces the production of inflammatory cytokines, abrogates gliosis and neuronal cell death, and eventually improves the survival rate in the mouse model. Collectively, our results demonstrate that miR-19b-3p positively regulates the JEV-induced inflammatory response. Thus, miR-19b-3p targeting may constitute a thought-provoking approach to rein in JEV-induced inflammation.Japanese encephalitis virus (JEV) is one of the major causes of acute encephalitis in humans worldwide. The pathological features of JEV-induced encephalitis are inflammatory reactions and neurological diseases resulting from glia activation. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression posttranscriptionally. Accumulating data indicate that miRNAs regulate a variety of cellular processes, including the host inflammatory response under pathological conditions. Recently, a few studies demonstrated the role of miRNAs in a JEV-induced inflammatory response in microglia; however, their role in an astrocyte-derived inflammatory response is largely unknown. The present study reveals that miR-19b-3p targets ring finger protein 11 in glia and promotes inflammatory cytokine production by enhancing nuclear factor kappa B activity in these cells. Moreover, administration of an miR-19b-3p-specific antagomir in JEV-infected mice reduces neuroinflammation and lethality. These findings suggest a new insight into the molecular mechanism of the JEV-induced inflammatory response and provide a possible therapeutic entry point for treating viral encephalitis."],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China."],"language":["eng"],"pubModel":["Electronic-Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Astrocytes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis Virus, Japanese"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis, Japanese"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DT"],"qualifierName":["drug therapy"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]},{"abbreviation":["MO"],"qualifierName":["mortality"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Models, Animal"]},{"majorTopic_YN":["N"],"descriptorName":["Carrier Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["NF-kappa B"]},{"majorTopic_YN":["N"],"descriptorName":["MicroRNAs"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Messenger"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Oligonucleotides"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Inflammation 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrS-J9hGurOzZIZ3","_score":1,"_source":{"id":["27149628"],"source":["MED"],"pmid":["27149628"],"pmcid":["PMC4858223"],"doi":["10.1371/journal.pntd.0004702"],"title":["Zika Virus: A New Animal Model for an Arbovirus."],"authorString":["Aman MJ, Kashanchi F."],"authorList":[{"author":[{"fullName":["Aman MJ"],"firstName":["M Javad"],"lastName":["Aman"],"initials":["MJ"],"affiliation":["Integrated BioTherapeutics, Inc., Gaithersburg, Maryland, United States of America."]},{"fullName":["Kashanchi F"],"firstName":["Fatah"],"lastName":["Kashanchi"],"initials":["F"],"affiliation":["Laboratory of Molecular Virology, George Mason University, Manassas, Virginia, United States of America."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004702"],"affiliation":["Integrated BioTherapeutics, Inc., Gaithersburg, Maryland, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858223?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858223"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004702"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-06"],"dateOfRevision":["2016-05-14"],"electronicPublicationDate":["2016-05-05"],"firstPublicationDate":["2016-05-05"],"cprojectID":"PMC4858223"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXVhOUS2orL1IQR","_score":1,"_source":{"id":["27088494"],"source":["MED"],"pmid":["27088494"],"pmcid":["PMC4861542"],"doi":["10.3201/eid2205.160290"],"title":["Projecting Month of Birth for At-Risk Infants after Zika Virus Disease Outbreaks."],"authorString":["Reefhuis J, Gilboa SM, Johansson MA, Valencia D, Simeone RM, Hills SL, Polen K, Jamieson DJ, Petersen LR, Honein MA."],"authorList":[{"author":[{"fullName":["Reefhuis J"],"firstName":["Jennita"],"lastName":["Reefhuis"],"initials":["J"]},{"fullName":["Gilboa SM"],"firstName":["Suzanne M"],"lastName":["Gilboa"],"initials":["SM"]},{"fullName":["Johansson MA"],"firstName":["Michael A"],"lastName":["Johansson"],"initials":["MA"]},{"fullName":["Valencia D"],"firstName":["Diana"],"lastName":["Valencia"],"initials":["D"]},{"fullName":["Simeone RM"],"firstName":["Regina M"],"lastName":["Simeone"],"initials":["RM"]},{"fullName":["Hills SL"],"firstName":["Susan L"],"lastName":["Hills"],"initials":["SL"]},{"fullName":["Polen K"],"firstName":["Kara"],"lastName":["Polen"],"initials":["K"]},{"fullName":["Jamieson DJ"],"firstName":["Denise J"],"lastName":["Jamieson"],"initials":["DJ"]},{"fullName":["Petersen LR"],"firstName":["Lyle R"],"lastName":["Petersen"],"initials":["LR"]},{"fullName":["Honein MA"],"firstName":["Margaret A"],"lastName":["Honein"],"initials":["MA"]}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["828-832"],"abstractText":["The marked increase in infants born with microcephaly in Brazil after a 2015 outbreak of Zika virus (Zika virus) disease suggests an association between maternal Zika virus infection and congenital microcephaly. To project the timing of delivery of infants born to mothers infected during early pregnancy in 1 city in Bahia State, Brazil, we incorporated data on reported Zika virus disease cases and microcephaly cases into a graphical schematic of weekly birth cohorts. We projected that these births would occur through February 2016. Applying similar projections to a hypothetical location at which Zika virus transmission started in November, we projected that full-term infants at risk for Zika virus infection would be born during April-September 2016. We also developed a modifiable spreadsheet tool that public health officials and researchers can use for their countries to plan for deliveries of infants to women who were infected with Zika virus during different pregnancy trimesters."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Pregnancy","Viruses","Brazil","Birth defects","Microcephaly","Vector-borne Infections","Flavivirus","Zika Virus","Zikav"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861542?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861542"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.160290"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861542"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXUhOUS2orL1IQQ","_score":1,"_source":{"id":["27163257"],"source":["MED"],"pmid":["27163257"],"pmcid":["PMC4862633"],"doi":["10.1371/journal.pntd.0004695"],"title":["The Viral Polymerase Inhibitor 7-Deaza-2'-C-Methyladenosine Is a Potent Inhibitor of In Vitro Zika Virus Replication and Delays Disease Progression in a Robust Mouse Infection Model."],"authorString":["Zmurko J, Marques RE, Schols D, Verbeken E, Kaptein SJ, Neyts J."],"authorList":[{"author":[{"fullName":["Zmurko J"],"firstName":["Joanna"],"lastName":["Zmurko"],"initials":["J"],"affiliation":["Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium."]},{"fullName":["Marques RE"],"firstName":["Rafael E"],"lastName":["Marques"],"initials":["RE"],"authorId":[{"_":"0000-0002-6949-0947","$":{"type":"ORCID"}}],"affiliation":["Universidade Federal de Minas Gerais, Belo Horizonte, Brazil."]},{"fullName":["Schols D"],"firstName":["Dominique"],"lastName":["Schols"],"initials":["D"],"affiliation":["Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium."]},{"fullName":["Verbeken E"],"firstName":["Erik"],"lastName":["Verbeken"],"initials":["E"],"affiliation":["Department of Pathology, University of Leuven and Leuven University Hospitals, Leuven, Belgium."]},{"fullName":["Kaptein SJ"],"firstName":["Suzanne J F"],"lastName":["Kaptein"],"initials":["SJ"],"affiliation":["Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium."]},{"fullName":["Neyts J"],"firstName":["Johan"],"lastName":["Neyts"],"initials":["J"],"affiliation":["Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6949-0947","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004695"],"abstractText":["Zika virus (ZIKV) is an emerging flavivirus typically causing a dengue-like febrile illness, but neurological complications, such as microcephaly in newborns, have potentially been linked to this viral infection. We established a panel of in vitro assays to allow the identification of ZIKV inhibitors and demonstrate that the viral polymerase inhibitor 7-deaza-2'-C-methyladenosine (7DMA) efficiently inhibits replication. Infection of AG129 (IFN-α/β and IFN-γ receptor knock-out) mice with ZIKV resulted in acute neutrophilic encephalitis with viral antigens accumulating in neurons of the brain and spinal cord. Additionally, high levels of viral RNA were detected in the spleen, liver and kidney, and levels of IFN-γ and IL-18 were systematically increased in serum of ZIKV-infected mice. Interestingly, the virus was also detected in testicles of infected mice. In line with its in vitro anti-ZIKV activity, 7DMA reduced viremia and delayed virus-induced morbidity and mortality in infected mice, which also validates this small animal model to assess the in vivo efficacy of novel ZIKV inhibitors. Since AG129 mice can generate an antibody response, and have been used in dengue vaccine studies, the model can also be used to assess the efficacy of ZIKV vaccines. ."],"affiliation":["Laboratory of Virology and Chemotherapy, KU Leuven - University of Leuven, Leuven, Belgium."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4862633?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4862633"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004695"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-11"],"dateOfRevision":["2016-05-19"],"electronicPublicationDate":["2016-05-10"],"firstPublicationDate":["2016-05-10"],"cprojectID":"PMC4862633"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXwJ9hGurOzZIaE","_score":1,"_source":{"id":["27247952"],"source":["MED"],"pmid":["27247952"],"pmcid":["PMC4867558"],"doi":["10.1002/mgg3.224"],"title":["Medical genetics and genomic medicine in the Dominican Republic: challenges and opportunities."],"authorString":["Estrada-Veras JI, Cabrera-Peña GA, Pérez-Estrella de Ferrán C."],"authorList":[{"author":[{"fullName":["Estrada-Veras JI"],"firstName":["Juvianee I"],"lastName":["Estrada-Veras"],"initials":["JI"],"affiliation":["Medical Genetics Branch National Human Genome Research Institute Section of Human Biochemical Genetics National Institutes of Health Bethesda Maryland."]},{"fullName":["Cabrera-Peña GA"],"firstName":["Giselle A"],"lastName":["Cabrera-Peña"],"initials":["GA"],"affiliation":["Primary Care Unit Carol Morgan School of Santo Domingo Santo Domingo Dominican Republic."]},{"fullName":["Pérez-Estrella de Ferrán C"],"firstName":["Ceila"],"lastName":["Pérez-Estrella de Ferrán"],"initials":["C"],"affiliation":["Department of Pediatrics Robert Reid Cabral Children's Hospital Santo Domingo Dominican Republic."]}]}],"journalInfo":[{"issue":["3"],"volume":["4"],"journalIssueId":["2413510"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Molecular genetics & genomic medicine"],"ISOAbbreviation":["Mol Genet Genomic Med"],"medlineAbbreviation":["Mol Genet Genomic Med"],"NLMid":["101603758"],"ESSN":["2324-9269"]}]}],"pubYear":["2016"],"pageInfo":["243-256"],"abstractText":["Medical genetics and genomic medicine in the Dominican Republic: challenges and opportunities."],"affiliation":["Medical Genetics Branch National Human Genome Research Institute Section of Human Biochemical Genetics National Institutes of Health Bethesda Maryland."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867558?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867558"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1002/mgg3.224"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-01"],"dateOfCreation":["2016-06-01"],"dateOfRevision":["2016-06-03"],"electronicPublicationDate":["2016-05-12"],"firstPublicationDate":["2016-05-12"],"cprojectID":"PMC4867558"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnro0hOUS2orL1IRR","_score":1,"_source":{"id":["27362709"],"source":["MED"],"pmid":["27362709"],"pmcid":["PMC4928868"],"doi":["10.1371/journal.pntd.0004759"],"title":["Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy."],"authorString":["Sikulu-Lord MT, Maia MF, Milali MP, Henry M, Mkandawile G, Kho EA, Wirtz RA, Hugo LE, Dowell FE, Devine GJ."],"authorList":[{"author":[{"fullName":["Sikulu-Lord MT"],"firstName":["Maggy T"],"lastName":["Sikulu-Lord"],"initials":["MT"],"authorId":[{"_":"0000-0002-9346-2970","$":{"type":"ORCID"}}],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."]},{"fullName":["Maia MF"],"firstName":["Marta F"],"lastName":["Maia"],"initials":["MF"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."]},{"fullName":["Milali MP"],"firstName":["Masabho P"],"lastName":["Milali"],"initials":["MP"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."]},{"fullName":["Henry M"],"firstName":["Michael"],"lastName":["Henry"],"initials":["M"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."]},{"fullName":["Mkandawile G"],"firstName":["Gustav"],"lastName":["Mkandawile"],"initials":["G"],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."]},{"fullName":["Kho EA"],"firstName":["Elise A"],"lastName":["Kho"],"initials":["EA"],"affiliation":["Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia."]},{"fullName":["Wirtz RA"],"firstName":["Robert A"],"lastName":["Wirtz"],"initials":["RA"],"affiliation":["Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America."]},{"fullName":["Hugo LE"],"firstName":["Leon E"],"lastName":["Hugo"],"initials":["LE"],"affiliation":["Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia."]},{"fullName":["Dowell FE"],"firstName":["Floyd E"],"lastName":["Dowell"],"initials":["FE"],"affiliation":["Stored Product Insect and Engineering Research Unit, United States Department of Agriculture/Agricultural Research Services, Center for Grain and Animal Health Research, Manhattan, Kansas, United States of America."]},{"fullName":["Devine GJ"],"firstName":["Gregor J"],"lastName":["Devine"],"initials":["GJ"],"affiliation":["Mosquito Control Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9346-2970","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004759"],"abstractText":["The release of Wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. We investigated the potential of using near-infrared spectroscopy (NIRS) to simultaneously detect and identify two strains of Wolbachia pipientis (wMelPop and wMel) in male and female laboratory-reared Aedes aegypti mosquitoes. Our aim is to find faster, cheaper alternatives for monitoring those releases than the molecular diagnostic techniques that are currently in use. Our findings indicate that NIRS can differentiate females and males infected with wMelPop from uninfected wild type samples with an accuracy of 96% (N = 299) and 87.5% (N = 377), respectively. Similarly, females and males infected with wMel were differentiated from uninfected wild type samples with accuracies of 92% (N = 352) and 89% (N = 444). NIRS could differentiate wMelPop and wMel transinfected females with an accuracy of 96.6% (N = 442) and males with an accuracy of 84.5% (N = 443). This non-destructive technique is faster than the standard polymerase chain reaction diagnostic techniques. After the purchase of a NIRS spectrometer, the technique requires little sample processing and does not consume any reagents."],"affiliation":["Environmental Health and Ecological Sciences Thematic Group, Ifakara Health Institute, Ifakara, United Republic of Tanzania."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928868?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928868"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004759"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-01"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-06-30"],"firstPublicationDate":["2016-06-30"],"cprojectID":"PMC4928868"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrcEhOUS2orL1IQn","_score":1,"_source":{"id":["27090089"],"source":["MED"],"pmid":["27090089"],"pmcid":["PMC4889326"],"doi":["10.7554/elife.15272"],"title":["Mapping global environmental suitability for Zika virus."],"authorString":["Messina JP, Kraemer MU, Brady OJ, Pigott DM, Shearer FM, Weiss DJ, Golding N, Ruktanonchai CW, Gething PW, Cohn E, Brownstein JS, Khan K, Tatem AJ, Jaenisch T, Murray CJ, Marinho F, Scott TW, Hay SI."],"authorList":[{"author":[{"fullName":["Messina JP"],"firstName":["Jane P"],"lastName":["Messina"],"initials":["JP"],"authorId":[{"_":"0000-0001-7829-1272","$":{"type":"ORCID"}}],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Kraemer MU"],"firstName":["Moritz Ug"],"lastName":["Kraemer"],"initials":["MU"],"authorId":[{"_":"0000-0001-8838-7147","$":{"type":"ORCID"}}],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Brady OJ"],"firstName":["Oliver J"],"lastName":["Brady"],"initials":["OJ"],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Pigott DM"],"firstName":["David M"],"lastName":["Pigott"],"initials":["DM"],"affiliation":["Institute for Health Metrics and Evaluation, University of Washington, Seattle, United States."]},{"fullName":["Shearer FM"],"firstName":["Freya M"],"lastName":["Shearer"],"initials":["FM"],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Weiss DJ"],"firstName":["Daniel J"],"lastName":["Weiss"],"initials":["DJ"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Golding N"],"firstName":["Nick"],"lastName":["Golding"],"initials":["N"],"affiliation":["Department of BioSciences, University of Melbourne, Parkville, United Kingdom."]},{"fullName":["Ruktanonchai CW"],"firstName":["Corrine W"],"lastName":["Ruktanonchai"],"initials":["CW"],"affiliation":["WorldPop project, Department of Geography and Environment, University of Southampton, Southampton, United Kingdom."]},{"fullName":["Gething PW"],"firstName":["Peter W"],"lastName":["Gething"],"initials":["PW"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Cohn E"],"firstName":["Emily"],"lastName":["Cohn"],"initials":["E"],"affiliation":["Boston Children's Hospital, Harvard Medical School, Boston, United Kingdom."]},{"fullName":["Brownstein JS"],"firstName":["John S"],"lastName":["Brownstein"],"initials":["JS"],"affiliation":["Boston Children's Hospital, Harvard Medical School, Boston, United Kingdom."]},{"fullName":["Khan K"],"firstName":["Kamran"],"lastName":["Khan"],"initials":["K"],"affiliation":["Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Canada."]},{"fullName":["Tatem AJ"],"firstName":["Andrew J"],"lastName":["Tatem"],"initials":["AJ"],"affiliation":["Flowminder Foundation, Stockholm, Sweden."]},{"fullName":["Jaenisch T"],"firstName":["Thomas"],"lastName":["Jaenisch"],"initials":["T"],"affiliation":["German Centre for Infection Research (DZIF), Heidelberg partner site, Heidelberg, Germany."]},{"fullName":["Murray CJ"],"firstName":["Christopher Jl"],"lastName":["Murray"],"initials":["CJ"],"affiliation":["Institute for Health Metrics and Evaluation, University of Washington, Seattle, United States."]},{"fullName":["Marinho F"],"firstName":["Fatima"],"lastName":["Marinho"],"initials":["F"],"affiliation":["Secretariat of Health Surveillance, Ministry of Health Brazil, Brasilia, Brazil."]},{"fullName":["Scott TW"],"firstName":["Thomas W"],"lastName":["Scott"],"initials":["TW"],"affiliation":["Department of Entomology and Nematology, University of California Davis, Davis, United States."]},{"fullName":["Hay SI"],"firstName":["Simon I"],"lastName":["Hay"],"initials":["SI"],"authorId":[{"_":"0000-0002-0611-7272","$":{"type":"ORCID"}}],"affiliation":["Institute for Health Metrics and Evaluation, University of Washington, Seattle, United States."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7829-1272","$":{"type":"ORCID"}},{"_":"0000-0001-8838-7147","$":{"type":"ORCID"}},{"_":"0000-0002-0611-7272","$":{"type":"ORCID"}},{"_":"0000-0002-7889-3473","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2360542"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["eLife"],"ISOAbbreviation":["Elife"],"medlineAbbreviation":["Elife"],"NLMid":["101579614"],"ESSN":["2050-084X"]}]}],"pubYear":["2016"],"abstractText":["Zika virus was discovered in Uganda in 1947 and is transmitted by Aedes mosquitoes, which also act as vectors for dengue and chikungunya viruses throughout much of the tropical world. In 2007, an outbreak in the Federated States of Micronesia sparked public health concern. In 2013, the virus began to spread across other parts of Oceania and in 2015, a large outbreak in Latin America began in Brazil. Possible associations with microcephaly and Guillain-Barré syndrome observed in this outbreak have raised concerns about continued global spread of Zika virus, prompting its declaration as a Public Health Emergency of International Concern by the World Health Organization. We conducted species distribution modelling to map environmental suitability for Zika. We show a large portion of tropical and sub-tropical regions globally have suitable environmental conditions with over 2.17 billion people inhabiting these areas."],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Human","Virus","Microbiology","Infectious disease","epidemiology","Global Health","Vector-borne Disease","Disease Mapping","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889326?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889326"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7554/eLife.15272"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-02"],"dateOfRevision":["2016-06-03"],"electronicPublicationDate":["2016-04-19"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4889326"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrds_8VqgcuJwvid","_score":1,"_source":{"id":["26926623"],"source":["MED"],"pmid":["26926623"],"pmcid":["PMC4868866"],"doi":["10.3758/s13415-016-0411-x"],"title":["The bilingual brain turns a blind eye to negative statements in the second language."],"authorString":["Jończyk R, Boutonnet B, Musiał K, Hoemann K, Thierry G."],"authorList":[{"author":[{"fullName":["Jończyk R"],"firstName":["Rafał"],"lastName":["Jończyk"],"initials":["R"],"affiliation":["Faculty of English, Adam Mickiewicz University, Poznań, Poland. rafal.jonczyk@gmail.com."]},{"fullName":["Boutonnet B"],"firstName":["Bastien"],"lastName":["Boutonnet"],"initials":["B"],"affiliation":["Leiden Institute for Brain and Cognition, Leiden University, Leiden, The Netherlands."]},{"fullName":["Musiał K"],"firstName":["Kamil"],"lastName":["Musiał"],"initials":["K"],"affiliation":["School of Psychology, Bangor University, Bangor, Wales, UK."]},{"fullName":["Hoemann K"],"firstName":["Katie"],"lastName":["Hoemann"],"initials":["K"],"affiliation":["Department of Psychology, Northeastern University, Boston, MA, USA."]},{"fullName":["Thierry G"],"firstName":["Guillaume"],"lastName":["Thierry"],"initials":["G"],"affiliation":["School of Psychology, Bangor University, Bangor, Wales, UK."]}]}],"journalInfo":[{"issue":["3"],"volume":["16"],"journalIssueId":["2409370"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Cognitive, affective & behavioral neuroscience"],"ISOAbbreviation":["Cogn Affect Behav Neurosci"],"medlineAbbreviation":["Cogn Affect Behav Neurosci"],"NLMid":["101083946"],"ISSN":["1530-7026"],"ESSN":["1531-135X"]}]}],"pubYear":["2016"],"pageInfo":["527-540"],"abstractText":["Neurobilingualism research has failed to reveal significant language differences in the processing of affective content. However, the evidence to date derives mostly from studies in which affective stimuli are presented out of context, which is unnatural and fails to capture the complexity of everyday sentence-based communication. Here we investigated semantic integration of affectively salient stimuli in sentential context in the first- and second-language (L2) of late fluent Polish-English bilinguals living in the UK. The 19 participants indicated whether Polish and English sentences ending with a semantically and affectively congruent or incongruent adjective of controlled affective valence made sense while undergoing behavioral and electrophysiological recordings. We focused on the N400, a wave of event-related potentials known to index semantic integration. We expected N400 amplitude to index increased processing demands in L2 English comprehension and potential language-valence interactions to reveal differences in affective processing between languages. Contrary to our initial expectation, we found increased N400 for sentences in L1 Polish, possibly driven by greater affective salience of sentences in the native language. Critically, language interacted with affective valence, such that N400 amplitudes were reduced for English sentences ending in a negative fashion as compared to all other conditions. We interpreted this as a sign that bilinguals suppress L2 content embedded in naturalistic L2 sentences when it has negative valence, thus extending the findings of previous research on single words in clinical and linguistic research."],"affiliation":["Faculty of English, Adam Mickiewicz University, Poznań, Poland. rafal.jonczyk@gmail.com."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["ERP","Affect","N400","L2","Lpc"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4868866?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4868866"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3758/s13415-016-0411-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-17"],"dateOfRevision":["2016-06-01"],"firstPublicationDate":["2016-02-29"],"cprojectID":"PMC4868866"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnreKJ9hGurOzZIac","_score":1,"_source":{"id":["27274588"],"source":["MED"],"pmid":["27274588"],"pmcid":["PMC4890216"],"doi":["10.2471/blt.16.176990"],"title":["Defining the syndrome associated with congenital Zika virus infection."],"authorString":["Costello A, Dua T, Duran P, Gülmezoglu M, Oladapo OT, Perea W, Pires J, Ramon-Pardo P, Rollins N, Saxena S."],"authorList":[{"author":[{"fullName":["Costello A"],"firstName":["Anthony"],"lastName":["Costello"],"initials":["A"],"affiliation":["Department of Maternal, Newborn, Child and Adolescent Health, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Dua T"],"firstName":["Tarun"],"lastName":["Dua"],"initials":["T"],"affiliation":["Department of Mental Health and Substance Abuse, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland ."]},{"fullName":["Duran P"],"firstName":["Pablo"],"lastName":["Duran"],"initials":["P"],"affiliation":["Center For Perinatology, Women and Reproductive Health, Pan American Health Organization/World Health Organization, Montevideo, Uruguay ."]},{"fullName":["Gülmezoglu M"],"firstName":["Metin"],"lastName":["Gülmezoglu"],"initials":["M"],"affiliation":["Department of Reproductive Health and Research, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Oladapo OT"],"firstName":["Olufemi T"],"lastName":["Oladapo"],"initials":["OT"],"affiliation":["Department of Reproductive Health and Research, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Perea W"],"firstName":["William"],"lastName":["Perea"],"initials":["W"],"affiliation":["Department of Pandemic and Epidemic Diseases, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Pires J"],"firstName":["João"],"lastName":["Pires"],"initials":["J"],"affiliation":["Division of Communicable Diseases and Health Security, World Health Organization Regional Office for Europe, Copenhagen, Denmark ."]},{"fullName":["Ramon-Pardo P"],"firstName":["Pilar"],"lastName":["Ramon-Pardo"],"initials":["P"],"affiliation":["Department of Communicable Diseases and Health Analysis, Pan American Health Organization/ World Health Organization, Washington, USA ."]},{"fullName":["Rollins N"],"firstName":["Nigel"],"lastName":["Rollins"],"initials":["N"],"affiliation":["Department of Maternal, Newborn, Child and Adolescent Health, World Health Organization, Geneva, Switzerland ."]},{"fullName":["Saxena S"],"firstName":["Shekhar"],"lastName":["Saxena"],"initials":["S"],"affiliation":["Department of Mental Health and Substance Abuse, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland ."]}]}],"journalInfo":[{"issue":["6"],"volume":["94"],"journalIssueId":["2415566"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["406-406A"],"affiliation":["Department of Maternal, Newborn, Child and Adolescent Health, World Health Organization, Geneva, Switzerland ."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890216?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890216"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.176990"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-10"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4890216"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfM_8VqgcuJwvik","_score":1,"_source":{"id":["27071041"],"source":["MED"],"pmid":["27071041"],"pmcid":["PMC4880105"],"doi":["10.3201/eid2206.160062"],"title":["Microcephaly in Infants, Pernambuco State, Brazil, 2015."],"authorString":["Microcephaly Epidemic Research Group1."],"authorList":[{"author":[{"collectiveName":["Microcephaly Epidemic Research Group1"]}]}],"journalInfo":[{"issue":["6"],"volume":["22"],"journalIssueId":["2397708"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1090-1093"],"abstractText":["We studied the clinical characteristics for 104 infants born with microcephaly in the delivery hospitals of Pernambuco State, Brazil, during 2015. Testing is ongoing to exclude known infectious causes. However, microcephaly peaked in October and demonstrated central nervous system abnormalities with brain dysgenesis and intracranial calcifications consistent with an intrauterine infection."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Viruses","Brazil","Infants","Microcephaly","Disease Outbreaks","Vector-borne Infections","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4880105?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4880105"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2206.160062"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-18"],"dateOfRevision":["2016-06-01"],"electronicPublicationDate":["2016-06-15"],"firstPublicationDate":["2016-06-15"],"embargoDate":["2016-06-01"],"cprojectID":"PMC4880105"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhyJ9hGurOzZIav","_score":1,"_source":{"id":["27314417"],"source":["MED"],"pmid":["27314417"],"pmcid":["PMC4918156"],"doi":["10.3201/eid2207.160469"],"title":["Yellow Fever in a Worker Returning to China from Angola, March 2016."],"authorString":["Ling Y, Chen J, Huang Q, Hu Y, Zhu A, Ye S, Xu L, Lu H."],"authorList":[{"author":[{"fullName":["Ling Y"],"firstName":["Yun"],"lastName":["Ling"],"initials":["Y"]},{"fullName":["Chen J"],"firstName":["Jun"],"lastName":["Chen"],"initials":["J"]},{"fullName":["Huang Q"],"firstName":["Qin"],"lastName":["Huang"],"initials":["Q"]},{"fullName":["Hu Y"],"firstName":["Yunwen"],"lastName":["Hu"],"initials":["Y"]},{"fullName":["Zhu A"],"firstName":["Aihong"],"lastName":["Zhu"],"initials":["A"]},{"fullName":["Ye S"],"firstName":["Shanke"],"lastName":["Ye"],"initials":["S"]},{"fullName":["Xu L"],"firstName":["Lie"],"lastName":["Xu"],"initials":["L"]},{"fullName":["Lu H"],"firstName":["Hongzhou"],"lastName":["Lu"],"initials":["H"]}]}],"journalInfo":[{"issue":["7"],"volume":["22"],"journalIssueId":["2397213"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1317-1318"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["China","Viruses","Vaccine","Yellow Fever","Angola","Yellow Fever Virus","Flavivirus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918156?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918156"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2207.160469"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-18"],"dateOfRevision":["2016-07-01"],"firstPublicationDate":["2016-07-01"],"embargoDate":["2016-07-01"],"cprojectID":"PMC4918156"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnri0_8VqgcuJwviv","_score":1,"_source":{"id":["27244249"],"source":["MED"],"pmid":["27244249"],"pmcid":["PMC4887023"],"doi":["10.1371/journal.pone.0156376"],"title":["Scoping Review of the Zika Virus Literature."],"authorString":["Waddell LA, Greig JD."],"authorList":[{"author":[{"fullName":["Waddell LA"],"firstName":["Lisa A"],"lastName":["Waddell"],"initials":["LA"],"affiliation":["Department of Population Medicine, University of Guelph, Guelph, Ontario, Canada."]},{"fullName":["Greig JD"],"firstName":["Judy D"],"lastName":["Greig"],"initials":["JD"],"affiliation":["National Microbiology Laboratory at Guelph, Public Health Agency of Canada, Guelph, Ontario, Canada."]}]}],"journalInfo":[{"issue":["5"],"volume":["11"],"journalIssueId":["2403348"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0156376"],"abstractText":["The global primary literature on Zika virus (ZIKV) (n = 233 studies and reports, up to March 1, 2016) has been compiled using a scoping review methodology to systematically identify and characterise the literature underpinning this broad topic using methods that are documented, updateable and reproducible. Our results indicate that more than half the primary literature on ZIKV has been published since 2011. The articles mainly covered three topic categories: epidemiology of ZIKV (surveillance and outbreak investigations) 56.6% (132/233), pathogenesis of ZIKV (case symptoms/ outcomes and diagnosis) 38.2% (89/233) and ZIKV studies (molecular characterisation and in vitro evaluation of the virus) 18.5% (43/233). There has been little reported in the primary literature on ZIKV vectors (12/233), surveillance for ZIKV (13/233), diagnostic tests (12/233) and transmission (10/233). Three papers reported on ZIKV prevention/control strategies, one investigated knowledge and attitudes of health professionals and two vector mapping studies were reported. The majority of studies used observational study designs, 89.7% (209/233), of which 62/233 were case studies or case series, while fewer (24/233) used experimental study designs. Several knowledge gaps were identified by this review with respect to ZIKV epidemiology, the importance of potential non-human primates and other hosts in the transmission cycle, the burden of disease in humans, and complications related to human infection with ZIKV. Historically there has been little research on ZIKV; however, given its current spread through Australasia and the Americas, research resources are now being allocated to close many of the knowledge gaps identified in this scoping review. Future updates of this project will probably demonstrate enhanced evidence and understanding of ZIKV and its impact on public health."],"affiliation":["Department of Population Medicine, University of Guelph, Guelph, Ontario, Canada."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887023?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887023"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0156376"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-01"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-31"],"firstPublicationDate":["2016-05-31"],"cprojectID":"PMC4887023"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrkT_8VqgcuJwvi0","_score":1,"_source":{"id":["27303640"],"source":["MED"],"pmid":["27303640"],"pmcid":["PMC4890307"],"doi":["10.12688/f1000research.8391.1"],"title":["Some vexations that challenge viral immunology."],"authorString":["Rouse BT, Mueller SN."],"authorList":[{"author":[{"fullName":["Rouse BT"],"firstName":["Barry T"],"lastName":["Rouse"],"initials":["BT"],"affiliation":["Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA."]},{"fullName":["Mueller SN"],"firstName":["Scott N"],"lastName":["Mueller"],"initials":["SN"],"affiliation":["Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Victoria, Australia."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"abstractText":["The field of viral immunology seeks to understand mechanisms of virus-host interaction with a view of applying this knowledge to the design of effective vaccines and immunomodulators that control viral infections. This brief review discusses several areas of the field that hold substantial promise for translation, but where further work is critically required to find solutions. We emphasize that our fundamental understanding of virus-host relationships is moving in leaps and bounds, but we lag behind in applying this knowledge to the successful control of many viral infections."],"affiliation":["Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Vaccine","immunology","Immunomodulators","Acquired Immunity","Viral Immunology"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890307?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890307"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8391.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-15"],"dateOfCreation":["2016-06-15"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-05-27"],"firstPublicationDate":["2016-05-27"],"cprojectID":"PMC4890307"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlg_8VqgcuJwvi3","_score":1,"_source":{"id":["27230852"],"source":["MED"],"pmid":["27230852"],"pmcid":["PMC4893354"],"doi":["10.1007/s00415-016-8165-5"],"title":["Zika virus and neurology: proving cause and effect."],"authorString":["Massey TH, Robertson NP."],"authorList":[{"author":[{"fullName":["Massey TH"],"firstName":["T H"],"lastName":["Massey"],"initials":["TH"],"affiliation":["Division of Psychological Medicine and Clinical Neuroscience, Department of Neurology, Cardiff University, University Hospital of Wales, Heath Park, Cardiff, CF14 4XN, UK."]},{"fullName":["Robertson NP"],"firstName":["N P"],"lastName":["Robertson"],"initials":["NP"],"affiliation":["Division of Psychological Medicine and Clinical Neuroscience, Department of Neurology, Cardiff University, University Hospital of Wales, Heath Park, Cardiff, CF14 4XN, UK. robertsonnp@cardiff.ac.uk."]}]}],"journalInfo":[{"issue":["6"],"volume":["263"],"journalIssueId":["2416400"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Journal of neurology"],"ISOAbbreviation":["J. Neurol."],"medlineAbbreviation":["J Neurol"],"NLMid":["0423161"],"ISSN":["0340-5354"],"ESSN":["1432-1459"]}]}],"pubYear":["2016"],"pageInfo":["1255-1257"],"affiliation":["Division of Psychological Medicine and Clinical Neuroscience, Department of Neurology, Cardiff University, University Hospital of Wales, Heath Park, Cardiff, CF14 4XN, UK."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4893354?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4893354"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s00415-016-8165-5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-06"],"dateOfRevision":["2016-06-21"],"firstPublicationDate":["2016-05-26"],"cprojectID":"PMC4893354"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlhhOUS2orL1IRG","_score":1,"_source":{"id":["27322644"],"source":["MED"],"pmid":["27322644"],"pmcid":["PMC4920588"],"doi":["10.1371/journal.pntd.0004755"],"title":["Clinical Survey of Dengue Virus Circulation in the Republic of Djibouti between 2011 and 2014 Identifies Serotype 3 Epidemic and Recommends Clinical Diagnosis Guidelines for Resource Limited Settings."],"authorString":["Le Gonidec E, Maquart M, Duron S, Savini H, Cazajous G, Vidal PO, Chenilleau MC, Roseau JB, Benois A, Dehan C, Kugelman J, Leparc-Goffart I, Védy S."],"authorList":[{"author":[{"fullName":["Le Gonidec E"],"firstName":["Erwan"],"lastName":["Le Gonidec"],"initials":["E"],"affiliation":["Emergency Department, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Maquart M"],"firstName":["Marianne"],"lastName":["Maquart"],"initials":["M"],"affiliation":["French National Reference Centre for Arbovirus-Armed Forces Biomedical Research Institute, Marseille, France."]},{"fullName":["Duron S"],"firstName":["Sandrine"],"lastName":["Duron"],"initials":["S"],"affiliation":["French Armed forces Centre for Epidemiology and Public Health (CESPA), GSBdD Marseille Aubagne, Marseille, France."]},{"fullName":["Savini H"],"firstName":["Hélène"],"lastName":["Savini"],"initials":["H"],"affiliation":["Department of Infectious and Tropical Diseases, Laveran Military Teaching Hospital, Marseille, France."]},{"fullName":["Cazajous G"],"firstName":["Geraldine"],"lastName":["Cazajous"],"initials":["G"],"affiliation":["Department of Medicine, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Vidal PO"],"firstName":["Pierre-Olivier"],"lastName":["Vidal"],"initials":["PO"],"affiliation":["Emergency Department, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Chenilleau MC"],"firstName":["Marie-Caroline"],"lastName":["Chenilleau"],"initials":["MC"],"affiliation":["Department of Medicine, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Roseau JB"],"firstName":["Jean-Baptiste"],"lastName":["Roseau"],"initials":["JB"],"affiliation":["Department of Medicine, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Benois A"],"firstName":["Alain"],"lastName":["Benois"],"initials":["A"],"affiliation":["Department of Anesthesiology and Intensive Care, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Dehan C"],"firstName":["Céline"],"lastName":["Dehan"],"initials":["C"],"affiliation":["Department of Biology, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]},{"fullName":["Kugelman J"],"firstName":["Jeffrey"],"lastName":["Kugelman"],"initials":["J"],"affiliation":["U.S. Army Medical Research and Materiel Command, Fort Detrick, Maryland, United States of America."]},{"fullName":["Leparc-Goffart I"],"firstName":["Isabelle"],"lastName":["Leparc-Goffart"],"initials":["I"],"affiliation":["French National Reference Centre for Arbovirus-Armed Forces Biomedical Research Institute, Marseille, France."]},{"fullName":["Védy S"],"firstName":["Serge"],"lastName":["Védy"],"initials":["S"],"affiliation":["Department of Biology, Bouffard Military Hospital, Djibouti, Republic of Djibouti."]}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004755"],"abstractText":["Dengue virus is endemic globally, throughout tropical and sub-tropical regions. While the number of epidemics due to the four DENV serotypes is pronounced in East Africa, the total number of cases reported in Africa (16 million infections) remained at low levels compared to Asia (70 million infections). The French Armed forces Health Service provides epidemiological surveillance support in the Republic of Djibouti through the Bouffard Military hospital. Between 2011 and 2014, clinical and biological data of suspected dengue syndromes were collected at the Bouffard Military hospital and analyzed to improve Dengue clinical diagnosis and evaluate its circulation in East Africa. Examining samples from patients that presented one or more Dengue-like symptoms the study evidenced 128 Dengue cases among 354 suspected cases (36.2% of the non-malarial Dengue-like syndromes). It also demonstrated the circulation of serotypes 1 and 2 and reports the first epidemic of serotype 3 infections in Djibouti which was found in all of the hospitalized patients in this study. Based on these results we have determined that screening for Malaria and the presence of the arthralgia, gastro-intestinal symptoms and lymphopenia < 1,000cell/ mm3 allows for negative predictive value and specificity of diagnosis in isolated areas superior to 80% up to day 6. This study also provides evidence for an epidemic of Dengue virus serotype 3 previously not detected in Djibouti."],"affiliation":["Emergency Department, Bouffard Military Hospital, Djibouti, Republic of Djibouti."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920588?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920588"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004755"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-21"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-06-20"],"firstPublicationDate":["2016-06-20"],"cprojectID":"PMC4920588"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmj_8VqgcuJwvi8","_score":1,"_source":{"id":["27330653"],"source":["MED"],"pmid":["27330653"],"pmcid":["PMC4899052"],"doi":["10.5811/westjem.2016.3.30188"],"title":["Identify-Isolate-Inform: A Tool for Initial Detection and Management of Zika Virus Patients in the Emergency Department."],"authorString":["Koenig KL, Almadhyan A, Burns MJ."],"authorList":[{"author":[{"fullName":["Koenig KL"],"firstName":["Kristi L"],"lastName":["Koenig"],"initials":["KL"],"affiliation":["University of California Irvine Medical Center, Department of Emergency Medicine, Center for Disaster Medical Sciences, Orange, California."]},{"fullName":["Almadhyan A"],"firstName":["Abdulmajeed"],"lastName":["Almadhyan"],"initials":["A"],"affiliation":["University of California Irvine Medical Center, Department of Emergency Medicine, Center for Disaster Medical Sciences, Orange, California; Qassim University, Department of Emergency Medicine, Saudi Arabia."]},{"fullName":["Burns MJ"],"firstName":["Michael J"],"lastName":["Burns"],"initials":["MJ"],"affiliation":["University of California Irvine Medical Center, Department of Emergency Medicine and Department of Medicine, Division of Infectious Diseases, Orange, California."]}]}],"journalInfo":[{"issue":["3"],"volume":["17"],"journalIssueId":["2421089"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["The western journal of emergency medicine"],"ISOAbbreviation":["West J Emerg Med"],"medlineAbbreviation":["West J Emerg Med"],"NLMid":["101476450"],"ISSN":["1936-900X"],"ESSN":["1936-9018"]}]}],"pubYear":["2016"],"pageInfo":["238-244"],"abstractText":["First isolated in 1947 from a monkey in the Zika forest in Uganda, and from mosquitoes in the same forest the following year, Zika virus has gained international attention due to concerns for infection in pregnant women potentially causing fetal microcephaly. More than one million people have been infected since the appearance of the virus in Brazil in 2015. Approximately 80% of infected patients are asymptomatic. An association with microcephaly and other birth defects as well as Guillain-Barre Syndrome has led to a World Health Organization declaration of Zika virus as a Public Health Emergency of International Concern in February 2016. Zika virus is a vector-borne disease transmitted primarily by the Aedes aegypti mosquito. Male to female sexual transmission has been reported and there is potential for transmission via blood transfusions. After an incubation period of 2-7 days, symptomatic patients develop rapid onset fever, maculopapular rash, arthralgia, and conjunctivitis, often associated with headache and myalgias. Emergency department (ED) personnel must be prepared to address concerns from patients presenting with symptoms consistent with acute Zika virus infection, especially those who are pregnant or planning travel to Zika-endemic regions, as well as those women planning to become pregnant and their partners. The identify-isolate-inform (3I) tool, originally conceived for initial detection and management of Ebola virus disease patients in the ED, and later adjusted for measles and Middle East Respiratory Syndrome, can be adapted for real-time use for any emerging infectious disease. This paper reports a modification of the 3I tool for initial detection and management of patients under investigation for Zika virus. Following an assessment of epidemiologic risk, including travel to countries with mosquitoes that transmit Zika virus, patients are further investigated if clinically indicated. If after a rapid evaluation, Zika or other arthropod-borne diseases are the only concern, isolation (contact, droplet, airborne) is unnecessary. Zika is a reportable disease and thus appropriate health authorities must be notified. The modified 3I tool will facilitate rapid analysis and triggering of appropriate actions for patients presenting to the ED at risk for Zika."],"affiliation":["University of California Irvine Medical Center, Department of Emergency Medicine, Center for Disaster Medical Sciences, Orange, California."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4899052?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4899052"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.5811/westjem.2016.3.30188"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-22"],"dateOfRevision":["2016-06-24"],"electronicPublicationDate":["2016-04-04"],"firstPublicationDate":["2016-04-04"],"cprojectID":"PMC4899052"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrod_8VqgcuJwvi_","_score":1,"_source":{"id":["27366020"],"source":["MED"],"pmid":["27366020"],"pmcid":["PMC4901014"],"doi":["10.3346/jkms.2016.31.7.1173"],"title":["First Imported Case of Zika Virus Infection into Korea."],"authorString":["Jang HC, Park WB, Kim UJ, Chun JY, Choi SJ, Choe PG, Jung SI, Jee Y, Kim NJ, Choi EH, Oh MD."],"authorList":[{"author":[{"fullName":["Jang HC"],"firstName":["Hee-Chang"],"lastName":["Jang"],"initials":["HC"],"authorId":[{"_":"0000-0002-3407-8493","$":{"type":"ORCID"}}],"affiliation":["Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Korea ."]},{"fullName":["Park WB"],"firstName":["Wan Beom"],"lastName":["Park"],"initials":["WB"],"authorId":[{"_":"0000-0003-0022-9625","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Kim UJ"],"firstName":["Uh Jin"],"lastName":["Kim"],"initials":["UJ"],"authorId":[{"_":"0000-0002-8463-6297","$":{"type":"ORCID"}}],"affiliation":["Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Korea ."]},{"fullName":["Chun JY"],"firstName":["June Young"],"lastName":["Chun"],"initials":["JY"],"authorId":[{"_":"0000-0001-9345-6645","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Choi SJ"],"firstName":["Su-Jin"],"lastName":["Choi"],"initials":["SJ"],"authorId":[{"_":"0000-0001-8732-3950","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Choe PG"],"firstName":["Pyoeng Gyun"],"lastName":["Choe"],"initials":["PG"],"authorId":[{"_":"0000-0001-6794-7918","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Jung SI"],"firstName":["Sook-In"],"lastName":["Jung"],"initials":["SI"],"authorId":[{"_":"0000-0002-1577-678X","$":{"type":"ORCID"}}],"affiliation":["Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Korea ."]},{"fullName":["Jee Y"],"firstName":["Youngmee"],"lastName":["Jee"],"initials":["Y"],"affiliation":["Center for Pathology and Immunology, National Institute of Health, Korea Centers for Disease Control and Prevention, Osong, Korea."]},{"fullName":["Kim NJ"],"firstName":["Nam-Joong"],"lastName":["Kim"],"initials":["NJ"],"authorId":[{"_":"0000-0001-6793-9467","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Choi EH"],"firstName":["Eun Hwa"],"lastName":["Choi"],"initials":["EH"],"authorId":[{"_":"0000-0002-5857-0749","$":{"type":"ORCID"}}],"affiliation":["Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea ."]},{"fullName":["Oh MD"],"firstName":["Myoung-Don"],"lastName":["Oh"],"initials":["MD"],"authorId":[{"_":"0000-0002-2344-7695","$":{"type":"ORCID"}}],"affiliation":["Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea ."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3407-8493","$":{"type":"ORCID"}},{"_":"0000-0003-0022-9625","$":{"type":"ORCID"}},{"_":"0000-0002-8463-6297","$":{"type":"ORCID"}},{"_":"0000-0001-9345-6645","$":{"type":"ORCID"}},{"_":"0000-0001-8732-3950","$":{"type":"ORCID"}},{"_":"0000-0001-6794-7918","$":{"type":"ORCID"}},{"_":"0000-0002-1577-678X","$":{"type":"ORCID"}},{"_":"0000-0001-6793-9467","$":{"type":"ORCID"}},{"_":"0000-0002-5857-0749","$":{"type":"ORCID"}},{"_":"0000-0002-2344-7695","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["31"],"journalIssueId":["2424591"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Journal of Korean medical science"],"ISOAbbreviation":["J. Korean Med. Sci."],"medlineAbbreviation":["J Korean Med Sci"],"NLMid":["8703518"],"ISSN":["1011-8934"],"ESSN":["1598-6357"]}]}],"pubYear":["2016"],"pageInfo":["1173-1177"],"abstractText":["Since Zika virus has been spreading rapidly in the Americas from 2015, the outbreak of Zika virus infection becomes a global health emergency because it can cause neurological complications and adverse fetal outcome including microcephaly. Here, we report clinical manifestations and virus isolation findings from a case of Zika virus infection imported from Brazil. The patient, 43-year-old Korean man, developed fever, myalgia, eyeball pain, and maculopapular rash, but not neurological manifestations. Zika virus was isolated from his semen, and reverse-transcriptase PCR was positive for the virus in the blood, urine, and saliva on the 7th day of the illness but was negative on the 21st day. He recovered spontaneously without any neurological complications. He is the first case of Zika virus infection in Korea imported from Brazil."],"affiliation":["Department of Infectious Diseases, Chonnam National University Medical School, Gwangju, Korea ."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Korea","Brazil","Travel","Virus Shedding","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4901014?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4901014"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3346/jkms.2016.31.7.1173"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-01"],"electronicPublicationDate":["2016-05-27"],"firstPublicationDate":["2016-05-27"],"cprojectID":"PMC4901014"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnro4J9hGurOzZIbB","_score":1,"_source":{"id":["27429931"],"source":["MED"],"pmid":["27429931"],"pmcid":["PMC4932805"],"doi":["10.4103/2231-4040.184593"],"title":["Evaluating bionanoparticle infused fungal metabolites as a novel antimicrobial agent."],"authorString":["Rajpal K, Aziz N, Prasad R, Varma RG, Varma A."],"authorList":[{"author":[{"fullName":["Rajpal K"],"firstName":["Kartikeya"],"lastName":["Rajpal"],"initials":["K"],"affiliation":["Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India."]},{"fullName":["Aziz N"],"firstName":["Nafe"],"lastName":["Aziz"],"initials":["N"],"affiliation":["Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India."]},{"fullName":["Prasad R"],"firstName":["Ram"],"lastName":["Prasad"],"initials":["R"],"affiliation":["Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India."]},{"fullName":["Varma RG"],"firstName":["Ramendra G"],"lastName":["Varma"],"initials":["RG"],"affiliation":["Department of Chemistry, P.M.B. Gujarati Science College, Indore, Madhya Pradesh, India."]},{"fullName":["Varma A"],"firstName":["Ajit"],"lastName":["Varma"],"initials":["A"],"affiliation":["Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India."]}]}],"journalInfo":[{"issue":["3"],"volume":["7"],"journalIssueId":["2431325"],"dateOfPublication":["2016 Jul-Sep"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Journal of advanced pharmaceutical technology & research"],"ISOAbbreviation":["J Adv Pharm Technol Res"],"medlineAbbreviation":["J Adv Pharm Technol Res"],"NLMid":["101537797"],"ISSN":["2231-4040"],"ESSN":["0976-2094"]}]}],"pubYear":["2016"],"pageInfo":["110-114"],"abstractText":["Therapeutic properties of fungal metabolites and silver nanoparticles have been well documented. While fungal metabolites have been used for centuries as medicinal drugs, potential of biogenic silver nanoparticles has recently received attention. We have evaluated the antimicrobial potential of Aspergillus terreus crude extract, silver nanoparticles and an amalgamation of both against four pathogenic bacterial strains. Antimicrobial activity of the following was evaluated - A. terreus extract, biogenic silver nanoparticles, and a mixture containing extract and nanoparticles. Four pathogenic bacteria - Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Bacillus cereus were used as test organisms. Phenol, flavonoid, and alkaloid content of extract were determined to understand the chemical profile of the fungus. The extract contained significantly high amounts of phenols, flavonoids, and alkaloids. The extract and biogenic silver nanoparticle exhibited significant antibacterial activity at concentrations of 10 μg/ml and 1 μg/ml, respectively. When used in combination, the extract-nanoparticle mixture showed equally potent antibacterial activity at a much lower concentration of 2.5 μg/ml extract + 0.5 μg/ml nanoparticle. Given its high antibacterial potential, the fungal extract can be a promising source of novel drug lead compounds. The extract - silver nanoparticle mixture exhibited synergism in their antibacterial efficacy. This property can be further used to formulate new age drugs."],"affiliation":["Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, India."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aspergillus terreus","Antibacterial","Biogenic Silver Nanoparticle","Rann Of Kutch"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4932805"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/2231-4040.184593"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-19"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4932805"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrpShOUS2orL1IRV","_score":1,"_source":{"id":["27429492"],"source":["MED"],"pmid":["27429492"],"pmcid":["PMC4933143"],"doi":["10.2471/blt.15.164749"],"title":["National public health law: a role for WHO in capacity-building and promoting transparency."],"authorString":["Marks-Sultan G, Tsai FJ, Anderson E, Kastler F, Sprumont D, Burris S."],"authorList":[{"author":[{"fullName":["Marks-Sultan G"],"firstName":["Géraldine"],"lastName":["Marks-Sultan"],"initials":["G"],"affiliation":["Institut de Droit de la Santé, Université de Neuchâtel, Avenue du 1er-Mars 26, 2000 Neuchâtel, Switzerland ."]},{"fullName":["Tsai FJ"],"firstName":["Feng-Jen"],"lastName":["Tsai"],"initials":["FJ"],"affiliation":["Masters Program in Global Health and Development, Taipei Medical University, Taiwan, China ."]},{"fullName":["Anderson E"],"firstName":["Evan"],"lastName":["Anderson"],"initials":["E"],"affiliation":["Center for Public Health Initiatives, University of Pennsylvania, Philadelphia, United States of America (USA)."]},{"fullName":["Kastler F"],"firstName":["Florian"],"lastName":["Kastler"],"initials":["F"],"affiliation":["Health and Law Institute, University of Paris Descartes, Paris, France ."]},{"fullName":["Sprumont D"],"firstName":["Dominique"],"lastName":["Sprumont"],"initials":["D"],"affiliation":["Institut de Droit de la Santé, Université de Neuchâtel, Avenue du 1er-Mars 26, 2000 Neuchâtel, Switzerland ."]},{"fullName":["Burris S"],"firstName":["Scott"],"lastName":["Burris"],"initials":["S"],"affiliation":["Beasley School of Law, Temple University, Philadelphia, USA ."]}]}],"journalInfo":[{"issue":["7"],"volume":["94"],"journalIssueId":["2430669"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["534-539"],"abstractText":["A robust health infrastructure in every country is the most effective long-term preparedness strategy for global health emergencies. This includes not only health systems and their human resources, but also countries' legal infrastructure for health: the laws and policies that empower, obligate and sometimes limit government and private action. The law is also an important tool in health promotion and protection. Public health professionals play important roles in health law - from the development of policies, through their enforcement, to the scientific evaluation of the health impact of laws. Member States are already mandated to communicate their national health laws and regulations to the World Health Organization (WHO). In this paper we propose that WHO has the authority and credibility to support capacity-building in the area of health law within Member States, and to make national laws easier to access, understand, monitor and evaluate. We believe a strong case can be made to donors for the funding of a public health law centre or unit, that has adequate staffing, is robustly networked with its regional counterparts and is integrated into the main work of WHO. The mission of the unit or centre would be to define and integrate scientific and legal expertise in public health law, both technical and programmatic, across the work of WHO, and to conduct and facilitate global health policy surveillance."],"affiliation":["Institut de Droit de la Santé, Université de Neuchâtel, Avenue du 1er-Mars 26, 2000 Neuchâtel, Switzerland ."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933143?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933143"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.15.164749"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"electronicPublicationDate":["2016-05-03"],"firstPublicationDate":["2016-05-03"],"cprojectID":"PMC4933143"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrqZJ9hGurOzZIbL","_score":1,"_source":{"id":["26998633"],"source":["MED"],"pmid":["26998633"],"pmcid":["PMC4941169"],"doi":["10.1136/archdischild-2016-310590"],"title":["Outbreak of Zika virus disease in the Americas and the association with microcephaly, congenital malformations and Guillain-Barre syndrome."],"authorString":["Ladhani SN, O'Connor C, Kirkbride H, Brooks T, Morgan D."],"authorList":[{"author":[{"fullName":["Ladhani SN"],"firstName":["Shamez N"],"lastName":["Ladhani"],"initials":["SN"],"affiliation":["National Infection Service, Public Health England, London, UK Paediatric Infectious Diseases Research Group, St. George's University of London, London, UK."]},{"fullName":["O'Connor C"],"firstName":["Catherine"],"lastName":["O'Connor"],"initials":["C"],"affiliation":["National Infection Service, Public Health England, London, UK."]},{"fullName":["Kirkbride H"],"firstName":["Hilary"],"lastName":["Kirkbride"],"initials":["H"],"affiliation":["National Infection Service, Public Health England, London, UK."]},{"fullName":["Brooks T"],"firstName":["Tim"],"lastName":["Brooks"],"initials":["T"],"affiliation":["Rare and Imported Pathogens Laboratory, Public Health England, Salisbury, UK."]},{"fullName":["Morgan D"],"firstName":["Dilys"],"lastName":["Morgan"],"initials":["D"],"affiliation":["National Infection Service, Public Health England, London, UK."]}]}],"journalInfo":[{"issue":["7"],"volume":["101"],"journalIssueId":["2420932"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Archives of disease in childhood"],"ISOAbbreviation":["Arch. Dis. Child."],"medlineAbbreviation":["Arch Dis Child"],"NLMid":["0372434"],"ISSN":["0003-9888"],"ESSN":["1468-2044"]}]}],"pubYear":["2016"],"pageInfo":["600-602"],"affiliation":["National Infection Service, Public Health England, London, UK Paediatric Infectious Diseases Research Group, St. George's University of London, London, UK."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Brazil","Guillain-Barre syndrome","Microcephaly","Trave","Zika Virus"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4941169?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4941169"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1136/archdischild-2016-310590"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-18"],"dateOfRevision":["2016-07-14"],"electronicPublicationDate":["2016-03-14"],"firstPublicationDate":["2016-03-14"],"cprojectID":"PMC4941169"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrrX_8VqgcuJwvjQ","_score":1,"_source":{"id":["27345730"],"source":["MED"],"pmid":["27345730"],"pmcid":["PMC4921808"],"doi":["10.1038/srep28699"],"title":["Neuroprotection mediated by inhibition of calpain during acute viral encephalitis."],"authorString":["Howe CL, LaFrance-Corey RG, Mirchia K, Sauer BM, McGovern RM, Reid JM, Buenz EJ."],"authorList":[{"author":[{"fullName":["Howe CL"],"firstName":["Charles L"],"lastName":["Howe"],"initials":["CL"],"affiliation":["Departments of Immunology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["LaFrance-Corey RG"],"firstName":["Reghann G"],"lastName":["LaFrance-Corey"],"initials":["RG"],"affiliation":["Departments of Neurology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["Mirchia K"],"firstName":["Kanish"],"lastName":["Mirchia"],"initials":["K"],"affiliation":["Departments of Neurology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["Sauer BM"],"firstName":["Brian M"],"lastName":["Sauer"],"initials":["BM"],"affiliation":["Neurobiology of Disease PhD program, Mayo Graduate School, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["McGovern RM"],"firstName":["Renee M"],"lastName":["McGovern"],"initials":["RM"],"affiliation":["Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["Reid JM"],"firstName":["Joel M"],"lastName":["Reid"],"initials":["JM"],"affiliation":["Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]},{"fullName":["Buenz EJ"],"firstName":["Eric J"],"lastName":["Buenz"],"initials":["EJ"],"affiliation":["Departments of Neurology, Mayo Clinic, Rochester, Minnesota, 55905 USA."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["28699"],"abstractText":["Neurologic complications associated with viral encephalitis, including seizures and cognitive impairment, are a global health issue, especially in children. We previously showed that hippocampal injury during acute picornavirus infection in mice is associated with calpain activation and is the result of neuronal death triggered by brain-infiltrating inflammatory monocytes. We therefore hypothesized that treatment with a calpain inhibitor would protect neurons from immune-mediated bystander injury. C57BL/6J mice infected with the Daniel's strain of Theiler's murine encephalomyelitis virus were treated with the FDA-approved drug ritonavir using a dosing regimen that resulted in plasma concentrations within the therapeutic range for calpain inhibition. Ritonavir treatment significantly reduced calpain activity in the hippocampus, protected hippocampal neurons from death, preserved cognitive performance, and suppressed seizure escalation, even when therapy was initiated 36 hours after disease onset. Calpain inhibition by ritonavir may be a powerful tool for preserving neurons and cognitive function and preventing neural circuit dysregulation in humans with neuroinflammatory disorders."],"affiliation":["Departments of Immunology, Mayo Clinic, Rochester, Minnesota, 55905 USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4921808?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4921808"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep28699"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-27"],"dateOfRevision":["2016-06-29"],"electronicPublicationDate":["2016-06-27"],"firstPublicationDate":["2016-06-27"],"cprojectID":"PMC4921808"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrs-_8VqgcuJwvjZ","_score":1,"_source":{"id":["27362353"],"source":["MED"],"pmid":["27362353"],"pmcid":["PMC4928862"],"doi":["10.1371/journal.ppat.1005625"],"title":["Dissecting Virus Infectious Cycles by Cryo-Electron Microscopy."],"authorString":["Lee KK, Gui L."],"authorList":[{"author":[{"fullName":["Lee KK"],"firstName":["Kelly K"],"lastName":["Lee"],"initials":["KK"],"affiliation":["Biological Structure Physics and Design Program, University of Washington, Seattle, Washington, United States of America."]},{"fullName":["Gui L"],"firstName":["Long"],"lastName":["Gui"],"initials":["L"],"affiliation":["Biological Structure Physics and Design Program, University of Washington, Seattle, Washington, United States of America."]}]}],"journalInfo":[{"issue":["6"],"volume":["12"],"journalIssueId":["2413990"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005625"],"affiliation":["Biological Structure Physics and Design Program, University of Washington, Seattle, Washington, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928862?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928862"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005625"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-01"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-06-30"],"firstPublicationDate":["2016-06-30"],"cprojectID":"PMC4928862"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtD_8VqgcuJwvjb","_score":1,"_source":{"id":["27363842"],"source":["MED"],"pmid":["27363842"],"pmcid":["PMC4929711"],"doi":["10.1186/s13071-016-1660-9"],"title":["Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae)."],"authorString":["Coon KL, Brown MR, Strand MR."],"authorList":[{"author":[{"fullName":["Coon KL"],"firstName":["Kerri L"],"lastName":["Coon"],"initials":["KL"],"affiliation":["Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA."]},{"fullName":["Brown MR"],"firstName":["Mark R"],"lastName":["Brown"],"initials":["MR"],"affiliation":["Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA."]},{"fullName":["Strand MR"],"firstName":["Michael R"],"lastName":["Strand"],"initials":["MR"],"affiliation":["Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA. mrstrand@uga.edu."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["375"],"abstractText":["Aedes aegypti and A. atropalpus are related mosquitoes that differ reproductively. Aedes aegypti must blood-feed to produce eggs (anautogenous) while A. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). We recently characterized the gut microbiota of A. aegypti and A. atropalpus that were reared identically in the laboratory. Here, we assessed the effects of specific members of the gut microbiota in A. aegypti and A. atropalpus on female fitness including egg production.Gnotobiotic A. aegypti and A. atropalpus larvae were colonized by specific members of the gut microbiota. Survival, development time, size and egg production for each treatment was then compared to axenic and conventionally reared larvae.Most species of bacteria we tested supported normal development and egg production by A. aegypti but only one betaproteobacterium, a Comamonas, supported development and egg production by A. atropalpus to equivalent levels as conventionally reared females. Aedes atropalpus females colonized by Comamonas contained similar stores of glycogen and protein as conventionally reared females, whereas females colonized by Aquitalea did not. Small differences in bacterial loads were detected between gnotobiotic and conventionally reared A. aegypti and A. atropalpus, but this variation did not correlate with the beneficial effects of Comamonas in A. atropalpus.Specific members of the gut microbiota more strongly affected survival, size and egg production by A. atropalpus than A. aegypti."],"affiliation":["Department of Entomology, The University of Georgia, 120 Cedar Street, 420 Biological Sciences, Athens, GA, 30602, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["R01 AI106892"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Development","Reproduction","Oogenesis","Clutch size","Microbiota"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4929711?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4929711"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1660-9"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-01"],"dateOfRevision":["2016-07-15"],"electronicPublicationDate":["2016-06-30"],"firstPublicationDate":["2016-06-30"],"cprojectID":"PMC4929711"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtRJ9hGurOzZIbZ","_score":1,"_source":{"id":["26994075"],"source":["MED"],"pmid":["26994075"],"pmcid":["PMC4959074"],"doi":["10.1128/aem.00275-16"],"title":["Wolbachia Modulates Lipid Metabolism in Aedes albopictus Mosquito Cells."],"authorString":["Molloy JC, Sommer U, Viant MR, Sinkins SP."],"authorList":[{"author":[{"fullName":["Molloy JC"],"firstName":["Jennifer C"],"lastName":["Molloy"],"initials":["JC"],"affiliation":["Nuffield Department of Medicine and Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Sommer U"],"firstName":["Ulf"],"lastName":["Sommer"],"initials":["U"],"affiliation":["NERC Biomolecular Analysis Facility-Metabolomics Node, School of Biosciences, University of Birmingham, Birmingham, United Kingdom."]},{"fullName":["Viant MR"],"firstName":["Mark R"],"lastName":["Viant"],"initials":["MR"],"affiliation":["NERC Biomolecular Analysis Facility-Metabolomics Node, School of Biosciences, University of Birmingham, Birmingham, United Kingdom."]},{"fullName":["Sinkins SP"],"firstName":["Steven P"],"lastName":["Sinkins"],"initials":["SP"],"affiliation":["Nuffield Department of Medicine and Department of Zoology, University of Oxford, Oxford, United Kingdom Biomedical and Life Sciences, Lancaster University, Lancaster, United Kingdom s.sinkins@lancaster.ac.uk."]}]}],"journalInfo":[{"issue":["10"],"volume":["82"],"journalIssueId":["2410783"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Applied and environmental microbiology"],"ISOAbbreviation":["Appl. Environ. Microbiol."],"medlineAbbreviation":["Appl Environ Microbiol"],"NLMid":["7605801"],"ISSN":["0099-2240"],"ESSN":["1098-5336"]}]}],"pubYear":["2016"],"pageInfo":["3109-3120"],"abstractText":["Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia in comparison to uninfected Aa23-T cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia Most significantly, almost all sphingolipid classes were depleted, and some reductions in diacylglycerols and phosphatidylcholines were also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve our understanding of the intracellular interactions between Wolbachia and mosquitoes.Mosquitoes transmit a variety of important viruses to humans, such as dengue virus and Zika virus. Certain strains of the intracellular bacterial genus called Wolbachia found in or introduced into mosquitoes can block the transmission of viruses, including dengue virus, but the mechanisms responsible are not well understood. We found substantial shifts in the cellular lipid profiles in the presence of these bacteria. Some lipid classes previously shown to be enriched in dengue virus-infected mosquito cells were depleted in the presence of Wolbachia, suggesting that Wolbachia may produce a cellular lipid environment that inhibits mosquito-borne viruses."],"affiliation":["Nuffield Department of Medicine and Department of Zoology, University of Oxford, Oxford, United Kingdom."],"language":["eng"],"pubModel":["Electronic-Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4959074?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4959074"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/AEM.00275-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["metabolights"]}],"dateOfCreation":["2016-05-21"],"dateOfRevision":["2016-07-26"],"electronicPublicationDate":["2016-05-02"],"firstPublicationDate":["2016-03-18"],"cprojectID":"PMC4959074"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtZ_8VqgcuJwvje","_score":1,"_source":{"id":["27419068"],"source":["MED"],"pmid":["27419068"],"pmcid":["PMC4932437"],"doi":["10.1016/j.gdata.2016.06.002"],"title":["Complete genome sequencing and comparative genomic analysis of functionally diverse Lysinibacillus sphaericus III(3)7."],"authorString":["Rey A, Silva-Quintero L, Dussán J."],"authorList":[{"author":[{"fullName":["Rey A"],"firstName":["Andrés"],"lastName":["Rey"],"initials":["A"],"affiliation":["Centro de Investigaciones Microbiologicas (CIMIC), Universidad de los Andes, Bogotá D.C., Cundinamarca, Colombia."]},{"fullName":["Silva-Quintero L"],"firstName":["Laura"],"lastName":["Silva-Quintero"],"initials":["L"],"affiliation":["Centro de Investigaciones Microbiologicas (CIMIC), Universidad de los Andes, Bogotá D.C., Cundinamarca, Colombia."]},{"fullName":["Dussán J"],"firstName":["Jenny"],"lastName":["Dussán"],"initials":["J"],"affiliation":["Centro de Investigaciones Microbiologicas (CIMIC), Universidad de los Andes, Bogotá D.C., Cundinamarca, Colombia."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2421948"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Genomics data"],"ISOAbbreviation":["Genom Data"],"medlineAbbreviation":["Genom Data"],"NLMid":["101634120"],"ESSN":["2213-5960"]}]}],"pubYear":["2016"],"pageInfo":["78-86"],"abstractText":["Lysinibacillus sphaericus III(3)7 is a native Colombian strain, the first one isolated from soil samples. This strain has shown high levels of pathogenic activity against Culex quinquefaciatus larvae in laboratory assays compared to other members of the same species. Using Pacific Biosciences sequencing technology we sequenced, annotated (de novo) and described the genome of strain III(3)7, achieving a complete genome sequence status. We then performed a comparative analysis between the newly sequenced genome and the ones previously reported for Colombian isolates L. sphaericus OT4b.31, CBAM5 and OT4b.25, with the inclusion of L. sphaericus C3-41 that has been used as a reference genome for most of previous genome sequencing projects. We concluded that L. sphaericus III(3)7 is highly similar with strain OT4b.25 and shares high levels of synteny with isolates CBAM5 and C3-41."],"affiliation":["Centro de Investigaciones Microbiologicas (CIMIC), Universidad de los Andes, Bogotá D.C., Cundinamarca, Colombia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Comparative genomics","complete genome sequencing","Lysinibacillus Sphaericus","Putative Extrachromosomal Elements"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4932437?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4932437"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.gdata.2016.06.002"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-15"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-06-26"],"firstPublicationDate":["2016-06-26"],"cprojectID":"PMC4932437"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrulhOUS2orL1IRq","_score":1,"_source":{"id":["27459322"],"source":["MED"],"pmid":["27459322"],"pmcid":["PMC4961380"],"doi":["10.1371/journal.pntd.0004842"],"title":["A Sequence of Flushing and Drying of Breeding Habitats of Aedes aegypti (L.) Prior to the Low Dengue Season in Singapore."],"authorString":["Seidahmed OM, Eltahir EA."],"authorList":[{"author":[{"fullName":["Seidahmed OM"],"firstName":["Osama M E"],"lastName":["Seidahmed"],"initials":["OM"],"affiliation":["Ralph M Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America."]},{"fullName":["Eltahir EA"],"firstName":["Elfatih A B"],"lastName":["Eltahir"],"initials":["EA"],"affiliation":["Ralph M Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America."]}]}],"journalInfo":[{"issue":["7"],"volume":["10"],"journalIssueId":["2424122"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004842"],"abstractText":["In dengue-endemic areas, transmission shows both a seasonal and interannual variability. To investigate how rainfall impacts dengue seasonality in Singapore, we carried out a longitudinal survey in the Geylang neighborhood from August 2014 to August 2015. The survey comprised of twice-weekly random inspections to outdoor breeding habitats and continuous monitoring for positive ones. In addition, observations of rainstorms were collected. Out of 6824 inspected habitats, 67 contained Aedes aegypti, 11 contained Aedes albopictus and 24 contained Culex spp. The main outdoors habitat of Aedes aegypti was storm drains (54/67). We found that 80% of breeding sites in drains (43/54) were lost after intense rainstorms related to the wet phase of the Northeast monsoon (NE) between November 2014 and early January 2015. Subsequently, 95% (41/43) of these flushed drains had dried out during the dry phase of the NE in late January-February 2015. A return in the outdoor breeding of Aedes aegypti was observed after the onset of Southwest monsoon (SW) between May and August 2015. There was also a reduction in productivity of breeding habitats for larvae and pupae after the onset of the NE. In wet equatorial regions like Singapore, rainfall varies with the monsoons. A monsoon-driven sequence of flushing and drying shapes the outdoor seasonal abundance of Aedes aegypti. This finding can be used to optimize vector control strategies and better understand dengue in the context of climate change."],"affiliation":["Ralph M Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4961380?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4961380"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004842"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-27"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-07-26"],"firstPublicationDate":["2016-07-26"],"cprojectID":"PMC4961380"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnry3_8VqgcuJwvjq","_score":1,"_source":{"id":["27429697"],"source":["MED"],"pmid":["27429697"],"pmcid":["PMC4944803"],"doi":["10.5811/westjem.2016.5.30326"],"title":["Check the Head: Emergency Ultrasound Diagnosis of Fetal Anencephaly."],"authorString":["Hall JW, Denne N, Minardi JJ, Williams D, Balcik BJ."],"authorList":[{"author":[{"fullName":["Hall JW 4th"],"firstName":["John W"],"lastName":["Hall"],"initials":["JW"],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."]},{"fullName":["Denne N"],"firstName":["Nicolas"],"lastName":["Denne"],"initials":["N"],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."]},{"fullName":["Minardi JJ"],"firstName":["Joseph J"],"lastName":["Minardi"],"initials":["JJ"],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."]},{"fullName":["Williams D"],"firstName":["Debra"],"lastName":["Williams"],"initials":["D"],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."]},{"fullName":["Balcik BJ"],"firstName":["B J"],"lastName":["Balcik"],"initials":["BJ"],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."]}]}],"journalInfo":[{"issue":["4"],"volume":["17"],"journalIssueId":["2430676"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["The western journal of emergency medicine"],"ISOAbbreviation":["West J Emerg Med"],"medlineAbbreviation":["West J Emerg Med"],"NLMid":["101476450"],"ISSN":["1936-900X"],"ESSN":["1936-9018"]}]}],"pubYear":["2016"],"pageInfo":["460-463"],"abstractText":["Early pregnancy complaints in emergency medicine are common. Emergency physicians (EP) increasingly employ ultrasound (US) in the evaluation of these complaints. As a result, it is likely that rare and important diagnoses will be encountered. We report a case of fetal anencephaly diagnosed by bedside emergency US in a patient presenting with first-trimester vaginal bleeding.A 33-year-old patient at 10 weeks gestation presented with vaginal bleeding. After initial history and physical examination, a bedside US was performed. The EP noted the abnormal appearance of the fetal cranium and anencephaly was suspected. This finding was confirmed by a consultative high-resolution fetal US. Making the diagnosis at the point of care allowed earlier detection and more comprehensive maternal counseling about pregnancy options. This particular patient underwent elective abortion which was able to be performed at an earlier gestation, thus decreasing maternal risk. If this diagnosis would not have been recognized by the EP at the point of care, it may not have been diagnosed until the second trimester, and lower-risk maternal options would not have been available."],"affiliation":["West Virginia University School of Medicine, Department of Emergency Medicine, Morgantown, West Virginia."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4944803?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4944803"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.5811/westjem.2016.5.30326"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"electronicPublicationDate":["2016-07-05"],"firstPublicationDate":["2016-07-05"],"cprojectID":"PMC4944803"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3EJ9hGurOzZIb1","_score":1,"_source":{"id":["27440927"],"source":["MED"],"pmid":["27440927"],"pmcid":["PMC4975820"],"doi":["10.1136/sextrans-2015-052377"],"title":["Navigating an outbreak: geospatial methods for STI outbreak investigations."],"authorString":["Smith CM, Emmett L."],"authorList":[{"author":[{"fullName":["Smith CM"],"firstName":["Catherine M"],"lastName":["Smith"],"initials":["CM"],"affiliation":["Department of Infectious Disease Informatics, Farr Institute of Health Informatics Research, UCL, London, UK."]},{"fullName":["Emmett L"],"firstName":["Lynsey"],"lastName":["Emmett"],"initials":["L"],"affiliation":["Field Epidemiology Service East, National Infection Service, Public Health England, Cambridge, UK."]}]}],"journalInfo":[{"issue":["5"],"volume":["92"],"journalIssueId":["2432167"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Sexually transmitted infections"],"ISOAbbreviation":["Sex Transm Infect"],"medlineAbbreviation":["Sex Transm Infect"],"NLMid":["9805554"],"ISSN":["1368-4973"],"ESSN":["1472-3263"]}]}],"pubYear":["2016"],"pageInfo":["327-328"],"affiliation":["Department of Infectious Disease Informatics, Farr Institute of Health Informatics Research, UCL, London, UK."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Editorial"]}],"keywordList":[{"keyword":["Surveillance","Epidemiology (General)","Communication Technologies"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4975820?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4975820"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1136/sextrans-2015-052377"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-21"],"dateOfRevision":["2016-08-19"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4975820"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr4FhOUS2orL1ISJ","_score":1,"_source":{"id":["27505002"],"source":["MED"],"pmid":["27505002"],"pmcid":["PMC4978396"],"doi":["10.1371/journal.pntd.0004901"],"title":["Zika Virus, a New Threat for Europe?"],"authorString":["Jupille H, Seixas G, Mousson L, Sousa CA, Failloux AB."],"authorList":[{"author":[{"fullName":["Jupille H"],"firstName":["Henri"],"lastName":["Jupille"],"initials":["H"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Department of Virology, Paris, France."]},{"fullName":["Seixas G"],"firstName":["Gonçalo"],"lastName":["Seixas"],"initials":["G"],"affiliation":["Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal."]},{"fullName":["Mousson L"],"firstName":["Laurence"],"lastName":["Mousson"],"initials":["L"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Department of Virology, Paris, France."]},{"fullName":["Sousa CA"],"firstName":["Carla A"],"lastName":["Sousa"],"initials":["CA"],"affiliation":["Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal."]},{"fullName":["Failloux AB"],"firstName":["Anna-Bella"],"lastName":["Failloux"],"initials":["AB"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Department of Virology, Paris, France."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004901"],"abstractText":["Since its emergence in 2007 in Micronesia and Polynesia, the arthropod-borne flavivirus Zika virus (ZIKV) has spread in the Americas and the Caribbean, following first detection in Brazil in May 2015. The risk of ZIKV emergence in Europe increases as imported cases are repeatedly reported. Together with chikungunya virus (CHIKV) and dengue virus (DENV), ZIKV is transmitted by Aedes mosquitoes. Any countries where these mosquitoes are present could be potential sites for future ZIKV outbreak. We assessed the vector competence of European Aedes mosquitoes (Aedes aegypti and Aedes albopictus) for the currently circulating Asian genotype of ZIKV.Two populations of Ae. aegypti from the island of Madeira (Funchal and Paul do Mar) and two populations of Ae. albopictus from France (Nice and Bar-sur-Loup) were challenged with an Asian genotype of ZIKV isolated from a patient in April 2014 in New Caledonia. Fully engorged mosquitoes were then maintained in insectary conditions (28°±1°C, 16h:8h light:dark cycle and 80% humidity). 16-24 mosquitoes from each population were examined at 3, 6, 9 and 14 days post-infection to estimate the infection rate, disseminated infection rate and transmission efficiency. Based on these experimental infections, we demonstrated that Ae. albopictus from France were not very susceptible to ZIKV.In combination with the restricted distribution of European Ae. albopictus, our results on vector competence corroborate the low risk for ZIKV to expand into most parts of Europe with the possible exception of the warmest regions bordering the Mediterranean coastline."],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Department of Virology, Paris, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4978396?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4978396"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004901"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-10"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-09"],"firstPublicationDate":["2016-08-09"],"cprojectID":"PMC4978396"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr51J9hGurOzZIcC","_score":1,"_source":{"id":["27510731"],"source":["MED"],"pmid":["27510731"],"pmcid":["PMC4980774"],"doi":["10.1186/s40249-016-0172-3"],"title":["A community-based prospective cohort study of dengue viral infection in Malaysia: the study protocol."],"authorString":["Jahan NK, Ahmad MP, Dhanoa A, Meng CY, Ming LW, Reidpath DD, Allotey P, Zaini A, Phipps ME, Fatt QK, Rabu AB, Sirajudeen R, Fatan AA, Ghafar FA, Ahmad HB, Othman I, SyedHassan S."],"authorList":[{"author":[{"fullName":["Jahan NK"],"firstName":["Nowrozy Kamar"],"lastName":["Jahan"],"initials":["NK"],"affiliation":["South East Asia Community Observatory (SEACO), 146 Jalan Sia Her Yam, Suite 601-606, Wisma Centrepoint, Segamat, Johor Darul Takzim, 85000, Malaysia. nowrozy.jahan@monash.edu."]},{"fullName":["Ahmad MP"],"firstName":["Mohtar Pungut"],"lastName":["Ahmad"],"initials":["MP"],"affiliation":["Segamat District Public Health Office, Ministry of Health Malaysia, Peti Surat 102, Jalan Gudang Ubat, Kampung Gubah, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Dhanoa A"],"firstName":["Amreeta"],"lastName":["Dhanoa"],"initials":["A"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Meng CY"],"firstName":["Cheong Yuet"],"lastName":["Meng"],"initials":["CY"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Ming LW"],"firstName":["Lau Wee"],"lastName":["Ming"],"initials":["LW"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Reidpath DD"],"firstName":["Daniel D"],"lastName":["Reidpath"],"initials":["DD"],"affiliation":["South East Asia Community Observatory (SEACO), 146 Jalan Sia Her Yam, Suite 601-606, Wisma Centrepoint, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Allotey P"],"firstName":["Pascale"],"lastName":["Allotey"],"initials":["P"],"affiliation":["South East Asia Community Observatory (SEACO), 146 Jalan Sia Her Yam, Suite 601-606, Wisma Centrepoint, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Zaini A"],"firstName":["Anuar"],"lastName":["Zaini"],"initials":["A"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Phipps ME"],"firstName":["Maude Elvira"],"lastName":["Phipps"],"initials":["ME"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Fatt QK"],"firstName":["Quek Kia"],"lastName":["Fatt"],"initials":["QK"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["Rabu AB"],"firstName":["Aman Bin"],"lastName":["Rabu"],"initials":["AB"],"affiliation":["Hospital Segamat, Ministry of Health Malaysia , KM 6, Jalan Genuang, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Sirajudeen R"],"firstName":["Rowther"],"lastName":["Sirajudeen"],"initials":["R"],"affiliation":["Hospital Segamat, Ministry of Health Malaysia , KM 6, Jalan Genuang, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Fatan AA"],"firstName":["Ahmad AbdulBasitz Ahmad"],"lastName":["Fatan"],"initials":["AA"],"affiliation":["Hospital Segamat, Ministry of Health Malaysia , KM 6, Jalan Genuang, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Ghafar FA"],"firstName":["Faidzal Adlee"],"lastName":["Ghafar"],"initials":["FA"],"affiliation":["Hospital Segamat, Ministry of Health Malaysia , KM 6, Jalan Genuang, Segamat, Johor Darul Takzim, 85000, Malaysia."]},{"fullName":["Ahmad HB"],"firstName":["Hamdan Bin"],"lastName":["Ahmad"],"initials":["HB"],"affiliation":["University Sains Malaysia, Gelugor, Penang, 11800, Malaysia."]},{"fullName":["Othman I"],"firstName":["Iekhsan"],"lastName":["Othman"],"initials":["I"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]},{"fullName":["SyedHassan S"],"firstName":["Sharifah"],"lastName":["SyedHassan"],"initials":["S"],"affiliation":["Infection and Immunity Cluster, Tropical Medicine and Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Petaling Jaya, 47500, Malaysia."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2376921"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infectious diseases of poverty"],"ISOAbbreviation":["Infect Dis Poverty"],"medlineAbbreviation":["Infect Dis Poverty"],"NLMid":["101606645"],"ESSN":["2049-9957"]}]}],"pubYear":["2016"],"pageInfo":["76"],"abstractText":["Globally, dengue infections constitute a significant public health burden. In recent decades, Malaysia has become a dengue hyper-endemic country with the co-circulation of the four dengue virus serotypes. The cyclical dominance of sub-types contributes to a pattern of major outbreaks. The consequences can be observed in the rising incidence of reported dengue cases and dengue related deaths. Understanding the complex interaction of the dengue virus, its human hosts and the mosquito vectors at the community level may help develop strategies for addressing the problem.A prospective cohort study will be conducted in Segamat district of Johor State in Peninsular Malaysia. Researchers received approval from the Malaysian Medical Research Ethics Committee and Monash University Human Research Ethics Committee. The study will be conducted at a Malaysian based health and demographic surveillance site over a 1 year period in three different settings (urban, semi-urban and rural). The study will recruit healthy adults (male and female) aged 18 years and over, from three ethnic groups (Malay, Chinese and Indian). The sample size calculated using the Fleiss method with continuity correction is 333. Sero-surveillance of participants will be undertaken to identify asymptomatic, otherwise healthy cases; cases with dengue fever who are managed as out-patients; and cases with dengue fever admitted to a hospital. A genetic analysis of the participants will be undertaken to determine whether there is a relationship between genetic predisposition and disease severity. A detailed medical history, past history of dengue infection, vaccination history against other flaviviruses such as Japanese encephalitis and Yellow fever, and the family history of dengue infection will also be collected. In addition, a mosquito surveillance will be carried out simultaneously in recruitment areas to determine the molecular taxonomy of circulating vectors.The research findings will estimate the burden of asymptomatic and symptomatic dengue at the community level. It will also examine the relationship between virus serotypes and host genotypes, and the association of the clinical manifestation of the early phase with the entire course of illness."],"affiliation":["South East Asia Community Observatory (SEACO), 146 Jalan Sia Her Yam, Suite 601-606, Wisma Centrepoint, Segamat, Johor Darul Takzim, 85000, Malaysia. nowrozy.jahan@monash.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Protocol","Dengue","Prospective Cohort Study","Community-based","Seaco","Health And Demographic Surveillance Site"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980774?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980774"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40249-016-0172-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-11"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-08-11"],"firstPublicationDate":["2016-08-11"],"cprojectID":"PMC4980774"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr6ahOUS2orL1ISS","_score":1,"_source":{"id":["27139219"],"source":["MED"],"pmid":["27139219"],"pmcid":["PMC4982178"],"doi":["10.3201/eid2208.160292"],"title":["Febrile or Exanthematous Illness Associated with Zika, Dengue, and Chikungunya Viruses, Panama."],"authorString":["Araúz D, De Urriola L, Jones J, Castillo M, Martínez A, Murillo E, Troncoso L, Chen M, Abrego L, Armién B, Pascale JM, Sosa N, López-Verges S, Moreno B."],"authorList":[{"author":[{"fullName":["Araúz D"],"firstName":["Dimelza"],"lastName":["Araúz"],"initials":["D"]},{"fullName":["De Urriola L"],"firstName":["Luis"],"lastName":["De Urriola"],"initials":["L"]},{"fullName":["Jones J"],"firstName":["José"],"lastName":["Jones"],"initials":["J"]},{"fullName":["Castillo M"],"firstName":["Marlene"],"lastName":["Castillo"],"initials":["M"]},{"fullName":["Martínez A"],"firstName":["Alexander"],"lastName":["Martínez"],"initials":["A"]},{"fullName":["Murillo E"],"firstName":["Edison"],"lastName":["Murillo"],"initials":["E"]},{"fullName":["Troncoso L"],"firstName":["Leonidas"],"lastName":["Troncoso"],"initials":["L"]},{"fullName":["Chen M"],"firstName":["María"],"lastName":["Chen"],"initials":["M"]},{"fullName":["Abrego L"],"firstName":["Leyda"],"lastName":["Abrego"],"initials":["L"]},{"fullName":["Armién B"],"firstName":["Blas"],"lastName":["Armién"],"initials":["B"]},{"fullName":["Pascale JM"],"firstName":["Juan M"],"lastName":["Pascale"],"initials":["JM"]},{"fullName":["Sosa N"],"firstName":["Néstor"],"lastName":["Sosa"],"initials":["N"]},{"fullName":["López-Verges S"],"firstName":["Sandra"],"lastName":["López-Verges"],"initials":["S"]},{"fullName":["Moreno B"],"firstName":["Brechla"],"lastName":["Moreno"],"initials":["B"]}]}],"journalInfo":[{"issue":["8"],"volume":["22"],"journalIssueId":["2403977"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1515-1517"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Dengue virus","Chikungunya virus","outbreak","Panama","Flavivirus","Febrile Illness","Zika Virus","Exanthematous Illness"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982178?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982178"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2208.160292"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-20"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-08-15"],"firstPublicationDate":["2016-08-15"],"cprojectID":"PMC4982178"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr7lJ9hGurOzZIcJ","_score":1,"_source":{"id":["27540724"],"source":["MED"],"pmid":["27540724"],"pmcid":["PMC4991785"],"doi":["10.1371/journal.pntd.0004924"],"title":["Dengue Sentinel Traveler Surveillance: Monthly and Yearly Notification Trends among Japanese Travelers, 2006-2014."],"authorString":["Fukusumi M, Arashiro T, Arima Y, Matsui T, Shimada T, Kinoshita H, Arashiro A, Takasaki T, Sunagawa T, Oishi K."],"authorList":[{"author":[{"fullName":["Fukusumi M"],"firstName":["Munehisa"],"lastName":["Fukusumi"],"initials":["M"],"affiliation":["Osaka University Graduate School of Medicine, Osaka, Japan."]},{"fullName":["Arashiro T"],"firstName":["Takeshi"],"lastName":["Arashiro"],"initials":["T"],"affiliation":["School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan."]},{"fullName":["Arima Y"],"firstName":["Yuzo"],"lastName":["Arima"],"initials":["Y"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Matsui T"],"firstName":["Tamano"],"lastName":["Matsui"],"initials":["T"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Shimada T"],"firstName":["Tomoe"],"lastName":["Shimada"],"initials":["T"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Kinoshita H"],"firstName":["Hitomi"],"lastName":["Kinoshita"],"initials":["H"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Arashiro A"],"firstName":["Ashley"],"lastName":["Arashiro"],"initials":["A"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Takasaki T"],"firstName":["Tomohiko"],"lastName":["Takasaki"],"initials":["T"],"affiliation":["Kanagawa Prefectural Institute of Public Health, Chigasaki, Japan."]},{"fullName":["Sunagawa T"],"firstName":["Tomimasa"],"lastName":["Sunagawa"],"initials":["T"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]},{"fullName":["Oishi K"],"firstName":["Kazunori"],"lastName":["Oishi"],"initials":["K"],"affiliation":["Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004924"],"abstractText":["Dengue is becoming an increasing threat to non-endemic countries. In Japan, the reported number of imported cases has been rising, and the first domestic dengue outbreak in nearly 70 years was confirmed in 2014, highlighting the need for greater situational awareness and better-informed risk assessment.Using national disease surveillance data and publically available traveler statistics, we compared monthly and yearly trends in the destination country-specific dengue notification rate per 100,000 Japanese travelers with those of domestic dengue cases in the respective country visited during 2006-2014. Comparisons were made for countries accounting for the majority of importations; yearly comparisons were restricted to countries where respective national surveillance data were publicly available.There were 1007 imported Japanese dengue cases (Bali, Indonesia (n = 202), the Philippines (n = 230), Thailand (n = 160), and India (n = 152)). Consistent with historic local dengue seasonality, monthly notification rate among travelers peaked in August in Thailand, September in the Philippines, and in Bali during April with a smaller peak in August. While the number of travelers to Bali was greatest in August, the notification rate was highest in April. Annually, trends in the notification rate among travelers to the Philippines and Thailand also closely reflected local notification trends.Travelers to dengue-endemic countries appear to serve as reliable \"sentinels\", with the trends in estimated risk of dengue infection among Japanese travelers closely reflecting local dengue trends, both seasonally and annually. Sentinel traveler surveillance can contribute to evidence-based pretravel advice, and help inform risk assessments and decision-making for importation and potentially for subsequent secondary transmission. As our approach takes advantage of traveler data that are readily available as a proxy denominator, sentinel traveler surveillance can be a practical surveillance tool that other countries could consider for implementation."],"affiliation":["Osaka University Graduate School of Medicine, Osaka, Japan."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991785?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991785"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004924"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-20"],"electronicPublicationDate":["2016-08-19"],"firstPublicationDate":["2016-08-19"],"cprojectID":"PMC4991785"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsAX_8VqgcuJwvkS","_score":1,"_source":{"id":["27482881"],"source":["MED"],"pmid":["27482881"],"pmcid":["PMC4976254"],"doi":["10.1089/hs.2016.0001"],"title":["A Role for Science in Responding to Health Crises."],"authorString":["Colf LA, Brothers R, Murata CE."],"authorList":[{"author":[{"fullName":["Colf LA"],"firstName":["Leremy A"],"lastName":["Colf"],"initials":["LA"]},{"fullName":["Brothers R"],"firstName":["Reginald"],"lastName":["Brothers"],"initials":["R"]},{"fullName":["Murata CE"],"firstName":["Christina E"],"lastName":["Murata"],"initials":["CE"]}]}],"journalInfo":[{"issue":["4"],"volume":["14"],"journalIssueId":["2436316"],"dateOfPublication":["2016 Jul-Aug"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Health security"],"ISOAbbreviation":["Health Secur"],"medlineAbbreviation":["Health Secur"],"NLMid":["101654694"],"ISSN":["2326-5094"],"ESSN":["2326-5108"]}]}],"pubYear":["2016"],"pageInfo":["272-279"],"abstractText":["The Department of Homeland Security's (DHS) Science and Technology (S&T) Directorate plays a role in public health that extends beyond biodefense. These responsibilities were exercised as part of the 2014-16 Ebola outbreak, leading to productive and beneficial contributions to the international public health response and improved operations in the United States. However, we and others have identified numerous areas for improvement. Based on our successes and lessons learned, we propose a number of ways that DHS, the interagency, and academia can act now to ensure improved responses to future public health crises. These include pre-developing scientific capabilities to respond agnostically to threats, and disease-specific master question lists to organize and inform initial efforts. We are generating DHS-specific playbooks and tools for anticipating future needs and capturing requests from DHS components and our national and international partners, where efforts will also be used to refine and exercise communication and information-sharing practices. These experiences and improvement efforts have encouraged discussions on the role of science in developing government policy, specifically responding to public health crises. We propose specific considerations for both scientists and government decision makers to ensure that the best available science is incorporated into policy and operational decisions to facilitate highly effective responses to future health crises."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976254?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976254"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1089/hs.2016.0001"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-03"],"dateOfRevision":["2016-08-18"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4976254"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsCq_8VqgcuJwvkd","_score":1,"_source":{"id":["27331627"],"source":["MED"],"pmid":["27331627"],"pmcid":["PMC4982162"],"doi":["10.3201/eid2208.160480"],"title":["Zika Virus Disease in Traveler Returning from Vietnam to Israel."],"authorString":["Meltzer E, Lustig Y, Leshem E, Levy R, Gottesman G, Weissmann R, Rabi DH, Hindiyeh M, Koren R, Mendelson E, Schwartz E."],"authorList":[{"author":[{"fullName":["Meltzer E"],"firstName":["Eyal"],"lastName":["Meltzer"],"initials":["E"]},{"fullName":["Lustig Y"],"firstName":["Yaniv"],"lastName":["Lustig"],"initials":["Y"]},{"fullName":["Leshem E"],"firstName":["Eyal"],"lastName":["Leshem"],"initials":["E"]},{"fullName":["Levy R"],"firstName":["Ran"],"lastName":["Levy"],"initials":["R"]},{"fullName":["Gottesman G"],"firstName":["Giora"],"lastName":["Gottesman"],"initials":["G"]},{"fullName":["Weissmann R"],"firstName":["Rotem"],"lastName":["Weissmann"],"initials":["R"]},{"fullName":["Rabi DH"],"firstName":["Duha Hejleh"],"lastName":["Rabi"],"initials":["DH"]},{"fullName":["Hindiyeh M"],"firstName":["Musa"],"lastName":["Hindiyeh"],"initials":["M"]},{"fullName":["Koren R"],"firstName":["Ravit"],"lastName":["Koren"],"initials":["R"]},{"fullName":["Mendelson E"],"firstName":["Ella"],"lastName":["Mendelson"],"initials":["E"]},{"fullName":["Schwartz E"],"firstName":["Eli"],"lastName":["Schwartz"],"initials":["E"]}]}],"journalInfo":[{"issue":["8"],"volume":["22"],"journalIssueId":["2403977"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1521-1522"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Vietnam","Mexico","Colombia","Travel","Israel","Dominican Republic","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982162?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982162"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2208.160480"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-20"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-08-15"],"firstPublicationDate":["2016-08-15"],"cprojectID":"PMC4982162"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDsJ9hGurOzZIca","_score":1,"_source":{"id":["27590311"],"source":["MED"],"pmid":["27590311"],"pmcid":["PMC5009685"],"doi":["10.1186/s12985-016-0606-3"],"title":["Characterization of a Chikungunya virus strain isolated from banked patients' sera."],"authorString":["Chalaem P, Chusri S, Fernandez S, Chotigeat W, Anguita J, Pal U, Promnares K."],"authorList":[{"author":[{"fullName":["Chalaem P"],"firstName":["Pattra"],"lastName":["Chalaem"],"initials":["P"],"affiliation":["Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand."]},{"fullName":["Chusri S"],"firstName":["Sarunyou"],"lastName":["Chusri"],"initials":["S"],"affiliation":["Division of Infectious Disease, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand."]},{"fullName":["Fernandez S"],"firstName":["Stefan"],"lastName":["Fernandez"],"initials":["S"],"affiliation":["Department of Virology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand."]},{"fullName":["Chotigeat W"],"firstName":["Wilaiwan"],"lastName":["Chotigeat"],"initials":["W"],"affiliation":["Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand."]},{"fullName":["Anguita J"],"firstName":["Juan"],"lastName":["Anguita"],"initials":["J"],"affiliation":["Ikerbasque, Basque Foundation for Science, 48011, Bilbao, Bizkaia, Spain."]},{"fullName":["Pal U"],"firstName":["Utpal"],"lastName":["Pal"],"initials":["U"],"affiliation":["Department of Veterinary Medicine and Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD, 20742, USA."]},{"fullName":["Promnares K"],"firstName":["Kamoltip"],"lastName":["Promnares"],"initials":["K"],"affiliation":["Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand. kpromnares@gmail.com."]}]}],"journalInfo":[{"issue":["1"],"volume":["13"],"journalIssueId":["2370131"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["150"],"abstractText":["Chikungunya virus (CHIKV) is a prevalent mosquito-borne pathogen that is emerging in many parts of the globe causing significant human morbidity. Here, we report the isolation and characterization of an infectious CHIKV from banked serum specimens of suspected patients from the 2009 epidemic in Thailand.Standard plaque assay was used for CHIKV isolation from the banked serum specimens. Isolated CHIKV was identified base on E1 structural gene sequence. Growth kinetic, infectivity, cell viability and cytokine gene expression throughout CHIKV infection in a permissive cell line, 293T cells, was performed using several approaches, including standard plaque assay, immunofluorescence assay, classical MTT assay, and quantitative real-time PCR. Two tailed Student's t test was used for evaluation statistically significance between the mean values of the groups.Based on the E1 structural gene sequence and phylogenetic analysis, we identified the virus as the CHIK/SBY8/10 isolate from Indonesia. Assessment of the growth kinetics, cytopathic effects as well as its ability to induce cellular immune responses suggested that the currently isolated CHIK/SBY8/10 virus is relatively more virulent than a known CHIKV vaccine strain, which also induces more dramatic proinflammatory responses.Our studies further add to the infectivity of a less-studied yet infectious CHIKV isolate as well as underscored the importance and utility of 293T cells as an excellent cell culture model for studying viral growth, CHIKV-induced inflammatory cellular responses and cell death. Together, these studies provide novel information on the CHIKV biology, infectivity and virus-cell interaction, which would help develop novel interventions against the infection."],"affiliation":["Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Cytokines","Chikungunya virus","293T cells","Gene Expression","Chik/sby8/10 Isolate","Chikv 181/Clone 25"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009685?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009685"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0606-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-09-03"],"dateOfRevision":["2016-09-06"],"electronicPublicationDate":["2016-09-02"],"firstPublicationDate":["2016-09-02"],"cprojectID":"PMC5009685"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsHO_8VqgcuJwvko","_score":1,"_source":{"id":["27534704"],"source":["MED"],"pmid":["27534704"],"pmcid":["PMC4993171"],"doi":["10.1111/1751-7915.12397"],"title":["New vaccines: challenges of discovery."],"authorString":["Mahmoud A."],"authorList":[{"author":[{"fullName":["Mahmoud A"],"firstName":["Adel"],"lastName":["Mahmoud"],"initials":["A"],"affiliation":["Molecular Biology and Public Policy, Princeton University, Princeton, NJ, 08544, USA."]}]}],"journalInfo":[{"issue":["5"],"volume":["9"],"journalIssueId":["2443655"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Microbial biotechnology"],"ISOAbbreviation":["Microb Biotechnol"],"medlineAbbreviation":["Microb Biotechnol"],"NLMid":["101316335"],"ESSN":["1751-7915"]}]}],"pubYear":["2016"],"pageInfo":["549-552"],"abstractText":["Vaccines have been a major component of preventing and controlling infectious diseases. The basis for discovery of what protects is reviewed as well as new attempts in utilizing Reverse Vaccinology, RNA-RNA methods and proteome analysis are adding significantly to our knowledge. The challenge of how to define protective and defined components of microbes is still hampering efforts to discover new vaccines. Recent excitement about immunotherapy of cancer opens the way to develop vaccines against multiple malignancies."],"affiliation":["Molecular Biology and Public Policy, Princeton University, Princeton, NJ, 08544, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4993171?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4993171"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1111/1751-7915.12397"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-23"],"dateOfRevision":["2016-09-01"],"electronicPublicationDate":["2016-08-18"],"firstPublicationDate":["2016-08-18"],"cprojectID":"PMC4993171"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsIm_8VqgcuJwvku","_score":1,"_source":{"id":["27610098"],"source":["MED"],"pmid":["27610098"],"pmcid":["PMC4996823"],"doi":["10.3389/fmicb.2016.01233"],"title":["Interplay between Inflammation and Cellular Stress Triggered by Flaviviridae Viruses."],"authorString":["Valadão AL, Aguiar RS, de Arruda LB."],"authorList":[{"author":[{"fullName":["Valadão AL"],"firstName":["Ana L C"],"lastName":["Valadão"],"initials":["AL"],"affiliation":["Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil."]},{"fullName":["Aguiar RS"],"firstName":["Renato S"],"lastName":["Aguiar"],"initials":["RS"],"affiliation":["Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil."]},{"fullName":["de Arruda LB"],"firstName":["Luciana B"],"lastName":["de Arruda"],"initials":["LB"],"affiliation":["Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2369092"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2016"],"pageInfo":["1233"],"abstractText":["The Flaviviridae family comprises several human pathogens, including Dengue, Zika, Yellow Fever, West Nile, Japanese Encephalitis viruses, and Hepatitis C Virus. Those are enveloped, single-stranded positive sense RNA viruses, which replicate mostly in intracellular compartments associated to endoplasmic reticulum (ER) and Golgi complex. Virus replication results in abundant viral RNAs and proteins, which are recognized by cellular mechanisms evolved to prevent virus infection, resulting in inflammation and stress responses. Virus RNA molecules are sensed by Toll-like receptors (TLRs), RIG-I-like receptors (RIG-I and MDA5) and RNA-dependent protein kinases (PKR), inducing the production of inflammatory mediators and interferons. Simultaneously, the synthesis of virus RNA and proteins are distinguished in different compartments such as mitochondria, ER and cytoplasmic granules, triggering intracellular stress pathways, including oxidative stress, unfolded protein response pathway, and stress granules assembly. Here, we review the new findings that connect the inflammatory pathways to cellular stress sensors and the strategies of Flaviviridae members to counteract these cellular mechanisms and escape immune response."],"affiliation":["Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Reactive oxygen species","ER stress","innate immune response","Flavivirus","Stress Granules"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996823?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996823"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2016.01233"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-09-09"],"dateOfCreation":["2016-09-09"],"electronicPublicationDate":["2016-08-25"],"firstPublicationDate":["2016-08-25"],"cprojectID":"PMC4996823"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqwm_8VqgcuJwvgk","_score":1,"_source":{"id":["25404070"],"source":["MED"],"pmid":["25404070"],"pmcid":["PMC4347352"],"doi":["10.4269/ajtmh.14-0374"],"title":["Incrimination of Aedes (Stegomyia) hensilli Farner as an epidemic vector of Chikungunya virus on Yap Island, Federated States of Micronesia, 2013."],"authorString":["Savage HM, Ledermann JP, Yug L, Burkhalter KL, Marfel M, Hancock WT."],"authorList":[{"author":[{"fullName":["Savage HM"],"firstName":["Harry M"],"lastName":["Savage"],"initials":["HM"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia HMS1@cdc.gov."]},{"fullName":["Ledermann JP"],"firstName":["Jeremy P"],"lastName":["Ledermann"],"initials":["JP"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia."]},{"fullName":["Yug L"],"firstName":["Laurence"],"lastName":["Yug"],"initials":["L"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia."]},{"fullName":["Burkhalter KL"],"firstName":["Kristen L"],"lastName":["Burkhalter"],"initials":["KL"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia."]},{"fullName":["Marfel M"],"firstName":["Maria"],"lastName":["Marfel"],"initials":["M"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia."]},{"fullName":["Hancock WT"],"firstName":["W Thane"],"lastName":["Hancock"],"initials":["WT"],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia."]}]}],"journalInfo":[{"issue":["2"],"volume":["92"],"journalIssueId":["2250028"],"dateOfPublication":["2015 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2015"],"printPublicationDate":["2015-02-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2015"],"pageInfo":["429-436"],"abstractText":["Two species of Aedes (Stegomyia) were collected in response to the first chikungunya virus (CHIKV) outbreak on Yap Island: the native species Ae. hensilli Farner and the introduced species Ae. aegypti (L.). Fourteen CHIKV-positive mosquito pools were detected. Six pools were composed of female Ae. hensilli, six pools were composed of female Ae. aegypti, one pool was composed of male Ae. hensilli, and one pool contained female specimens identified as Ae. (Stg.) spp. Infection rates were not significantly different between female Ae. hensilli and Ae. aegypti. The occurrence of human cases in all areas of Yap Island and the greater number of sites that yielded virus from Ae. hensilli combined with the ubiquitous distribution of this species incriminate Ae. hensilli as the most important vector of CHIKV during the outbreak. Phylogenic analysis shows that virus strains on Yap are members of the Asia lineage and closely related to strains currently circulating in the Caribbean."],"affiliation":["Centers for Disease Control and Prevention, Fort Collins, Colorado; Yap Hospital and Yap State Department of Health, Yap, Federated States of Micronesia HMS1@cdc.gov."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Micronesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Epidemics"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["DNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4347352?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4347352"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.14-0374"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-04-20"],"dateOfCreation":["2015-02-05"],"dateOfRevision":["2015-03-21"],"electronicPublicationDate":["2014-11-17"],"firstPublicationDate":["2014-11-17"],"cprojectID":"PMC4347352"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSaJ9hGurOzZIXs","_score":1,"_source":{"id":["24225644"],"source":["MED"],"pmid":["24225644"],"pmcid":["PMC3863887"],"doi":["10.3390/ijerph10116049"],"title":["Flaviviruses in Europe: complex circulation patterns and their consequences for the diagnosis and control of West Nile disease."],"authorString":["Beck C, Jimenez-Clavero MA, Leblond A, Durand B, Nowotny N, Leparc-Goffart I, Zientara S, Jourdain E, Lecollinet S."],"authorList":[{"author":[{"fullName":["Beck C"],"firstName":["Cécile"],"lastName":["Beck"],"initials":["C"],"affiliation":["UMR1161 Virologie INRA, ANSES, ENVA, EU-RL on equine West Nile disease, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France. sylvie.lecollinet@anses.fr."]},{"fullName":["Jimenez-Clavero MA"],"firstName":["Miguel Angel"],"lastName":["Jimenez-Clavero"],"initials":["MA"],"authorId":[{"_":"0000-0003-2125-9743","$":{"type":"ORCID"}}]},{"fullName":["Leblond A"],"firstName":["Agnès"],"lastName":["Leblond"],"initials":["A"]},{"fullName":["Durand B"],"firstName":["Benoît"],"lastName":["Durand"],"initials":["B"],"authorId":[{"_":"0000-0003-0669-1394","$":{"type":"ORCID"}}]},{"fullName":["Nowotny N"],"firstName":["Norbert"],"lastName":["Nowotny"],"initials":["N"]},{"fullName":["Leparc-Goffart I"],"firstName":["Isabelle"],"lastName":["Leparc-Goffart"],"initials":["I"]},{"fullName":["Zientara S"],"firstName":["Stéphan"],"lastName":["Zientara"],"initials":["S"]},{"fullName":["Jourdain E"],"firstName":["Elsa"],"lastName":["Jourdain"],"initials":["E"]},{"fullName":["Lecollinet S"],"firstName":["Sylvie"],"lastName":["Lecollinet"],"initials":["S"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2125-9743","$":{"type":"ORCID"}},{"_":"0000-0003-0669-1394","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["11"],"volume":["10"],"journalIssueId":["2091915"],"dateOfPublication":["2013 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2013"],"printPublicationDate":["2013-11-01"],"journal":[{"title":["International journal of environmental research and public health"],"ISOAbbreviation":["Int J Environ Res Public Health"],"medlineAbbreviation":["Int J Environ Res Public Health"],"NLMid":["101238455"],"ISSN":["1661-7827"],"ESSN":["1660-4601"]}]}],"pubYear":["2013"],"pageInfo":["6049-6083"],"abstractText":["In Europe, many flaviviruses are endemic (West Nile, Usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). Due to the temporal and geographical co-circulation of flaviviruses in Europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. Serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the Flavivirus genus. Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. Serological assay results should thus be interpreted with care and confirmed by comparative neutralization tests using a panel of viruses known to circulate in Europe. However, antibody cross-reactivity could be advantageous in efforts to control emerging flaviviruses because it ensures partial cross-protection. In contrast, it might also facilitate subsequent diseases, through a phenomenon called antibody-dependent enhancement mainly described for dengue virus infections. Here, we review the serological methods commonly used in WNV diagnosis and surveillance in Europe. By examining past and current epidemiological situations in different European countries, we present the challenges involved in interpreting flavivirus serological tests and setting up appropriate surveillance programs; we also address the consequences of flavivirus circulation and vaccination for host immunity."],"affiliation":["UMR1161 Virologie INRA, ANSES, ENVA, EU-RL on equine West Nile disease, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France. sylvie.lecollinet@anses.fr."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["West Nile virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["West Nile Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TH"],"qualifierName":["therapy"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Serologic Tests"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Europe"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3863887?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3863887"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24225644"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24225644&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijerph10116049"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["18"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-06-06"],"dateOfCreation":["2013-11-14"],"electronicPublicationDate":["2013-11-12"],"firstPublicationDate":["2013-11-12"],"cprojectID":"PMC3863887"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSaJ9hGurOzZIXt","_score":1,"_source":{"id":["22447371"],"source":["MED"],"pmid":["22447371"],"pmcid":["PMC3929025"],"doi":["10.1007/s10943-012-9597-6"],"title":["Do existential variables mediate between religious-spiritual facets of functionality and psychological wellbeing."],"authorString":["Wnuk M, Marcinkowski JT."],"authorList":[{"author":[{"fullName":["Wnuk M"],"firstName":["Marcin"],"lastName":["Wnuk"],"initials":["M"],"affiliation":["Department of Social Medicine, Poznań University of Medical Sciences, Rokietnicka Street 5C, 60-806, Poznan, Poland, marwnuk@wp.pl."]},{"fullName":["Marcinkowski JT"],"firstName":["Jerzy Tadeusz"],"lastName":["Marcinkowski"],"initials":["JT"]}]}],"journalInfo":[{"issue":["1"],"volume":["53"],"journalIssueId":["2118895"],"dateOfPublication":["2014 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2014"],"printPublicationDate":["2014-02-01"],"journal":[{"title":["Journal of religion and health"],"ISOAbbreviation":["J Relig Health"],"medlineAbbreviation":["J Relig Health"],"NLMid":["2985199R"],"ISSN":["0022-4197"],"ESSN":["1573-6571"]}]}],"pubYear":["2014"],"pageInfo":["56-67"],"abstractText":["Religiosity has been related to psychological wellbeing outcomes. Although this relationship is primarily based on studies of church attendance or prayer and wellbeing, more recent work has focused on the potential mechanisms that may mediate the religion-wellbeing findings. One of the major function of religion is finding of meaning of life and improving hope. Recent studies have indicated that hope and meaning of life are the potential variables mediate between religion and wellbeing. It was hypothesized that one pathway through which religiosity may exert its positive influence on psychological wellbeing is through finding meaning of life and improving hope. One study was conducted examining the relationships among spiritual experiences, hope, meaning of life and psychological wellbeing operationalized as satisfaction with life, positive affect and negative affect. The following research tools were used: Daily Spiritual Experiences Scale, Purpose in Life Test, Hearth Hope Index, Cantril Ladder, Positive and Negative Affect Schedule. Meaning of life and hope were noticed to mediate between spiritual experiences and satisfaction with life as well as between spiritual experiences and positive affect. Spiritual experiences were not related to negative affect. Both meaning of life and hope predicted negative affect. This study found meaning of life and hope to be an important factors in the religion-wellbeing relationship and related to positive psychological outcomes, including improved satisfaction with life and positive affect as well as reduced negative affect."],"affiliation":["Department of Social Medicine, Poznań University of Medical Sciences, Rokietnicka Street 5C, 60-806, Poznan, Poland, marwnuk@wp.pl."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Adaptation, Psychological"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Personal Satisfaction"]},{"majorTopic_YN":["N"],"descriptorName":["Affect"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Mental Health"]},{"majorTopic_YN":["Y"],"descriptorName":["Religion and Psychology"]},{"majorTopic_YN":["N"],"descriptorName":["Students"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Spiritualism"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Poland"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Hope"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3929025?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3929025"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22447371&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22447371"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s10943-012-9597-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-04-10"],"dateOfCreation":["2014-01-24"],"firstPublicationDate":["2014-02-01"],"cprojectID":"PMC3929025"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSchOUS2orL1IN4","_score":1,"_source":{"id":["22701115"],"source":["MED"],"pmid":["22701115"],"pmcid":["PMC3369199"],"doi":["10.3389/fmicb.2012.00204"],"title":["Feedback Interactions between Trace Metal Nutrients and Phytoplankton in the Ocean."],"authorString":["Sunda WG."],"authorList":[{"author":[{"fullName":["Sunda WG"],"firstName":["William G"],"lastName":["Sunda"],"initials":["WG"]}]}],"journalInfo":[{"volume":["3"],"journalIssueId":["1899249"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2012"],"pageInfo":["204"],"abstractText":["In addition to control by major nutrient elements (nitrogen, phosphorus, and silicon) the productivity and species composition of marine phytoplankton communities are also regulated by a number of trace metal nutrients (iron, zinc, cobalt, manganese, copper, and cadmium). Of these, iron is most limiting to phytoplankton growth and has the greatest effect on algal species diversity. It also plays an important role in limiting di-nitrogen (N(2)) fixation rates, and thus is important in controlling ocean inventories of fixed nitrogen. Because of these effects, iron is thought to play a key role in regulating biological cycles of carbon and nitrogen in the ocean, including the biological transfer of carbon to the deep sea, the so-called biological CO(2) pump, which helps regulate atmospheric CO(2) and CO(2)-linked global warming. Other trace metal nutrients (zinc, cobalt, copper, and manganese) have lesser effects on productivity; but may exert an important influence on the species composition of algal communities because of large differences in metal requirements among species. The interactions between trace metals and ocean plankton are reciprocal: not only do the metals control the plankton, but the plankton regulate the distributions, chemical speciation, and cycling of these metals through cellular uptake and recycling processes, downward flux of biogenic particles, biological release of organic chelators, and mediation of redox reactions. This two way interaction has influenced not only the biology and chemistry of the modern ocean, but has had a profound influence on biogeochemistry of the ocean and earth system as a whole, and on the evolution of marine and terrestrial biology over geologic history."],"affiliation":["National Ocean Service, National Oceanic and Atmospheric Administration Beaufort, NC, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Iron","Cadmium","Zinc","Cobalt","Manganese","Phytoplankton","Trace Metal Nutrients","Trace Metal Chemistry"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3369199?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3369199"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22701115&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22701115"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2012.00204"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["11"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2012-10-02"],"dateOfCreation":["2012-06-15"],"dateOfRevision":["2013-08-13"],"electronicPublicationDate":["2012-06-07"],"firstPublicationDate":["2012-06-07"],"cprojectID":"PMC3369199"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSehOUS2orL1IN7","_score":1,"_source":{"id":["19915965"],"source":["MED"],"pmid":["19915965"],"pmcid":["PMC2937152"],"doi":["10.1007/s10964-009-9475-z"],"title":["Meaning in life as a mediator of ethnic identity and adjustment among adolescents from Latin, Asian, and European American backgrounds."],"authorString":["Kiang L, Fuligni AJ."],"authorList":[{"author":[{"fullName":["Kiang L"],"firstName":["Lisa"],"lastName":["Kiang"],"initials":["L"]},{"fullName":["Fuligni AJ"],"firstName":["Andrew J"],"lastName":["Fuligni"],"initials":["AJ"]}]}],"journalInfo":[{"issue":["11"],"volume":["39"],"journalIssueId":["1761489"],"dateOfPublication":["2010 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2010"],"printPublicationDate":["2010-11-01"],"journal":[{"title":["Journal of youth and adolescence"],"ISOAbbreviation":["J Youth Adolesc"],"medlineAbbreviation":["J Youth Adolesc"],"NLMid":["0333507"],"ISSN":["0047-2891"],"ESSN":["1573-6601"]}]}],"pubYear":["2010"],"pageInfo":["1253-1264"],"abstractText":["Establishing a sense of life meaning is a primary facet of well-being, yet is understudied in adolescent development. Using data from 579 adolescents (53% female) from Latin American, Asian, and European backgrounds, demographic differences in meaning in life, links with psychological and academic adjustment, and the role of meaning in explaining associations between ethnic identity and adjustment were examined. Although no generational or gender differences were found, Asian Americans reported higher search for meaning than Latin and European Americans. Presence of meaning was positively associated with self-esteem, academic adjustment, daily well-being, and ethnic belonging and exploration, whereas search for meaning was related to lower self-esteem and less stability in daily well-being. Presence of meaning mediated associations between ethnic identity and adjustment, explaining 28-52% of ethnic identity's protective effect on development. Ethnic identity thus appears to affect adjustment, in part, through its role in fostering a positive sense of meaning in adolescents' lives."],"affiliation":["Wake Forest University, Winston-Salem, NC 27109, USA. KiangL@wfu.edu"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent Behavior"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Social Adjustment"]},{"majorTopic_YN":["Y"],"descriptorName":["Social Identification"]},{"majorTopic_YN":["Y"],"descriptorName":["Adolescent Development"]},{"majorTopic_YN":["N"],"descriptorName":["Self Concept"]},{"majorTopic_YN":["N"],"descriptorName":["Cross-Cultural Comparison"]},{"majorTopic_YN":["N"],"descriptorName":["Quality of Life"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Value of Life"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Asian Americans"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["Y"]},{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["European Continental Ancestry Group"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["Y"]},{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ethnic Groups"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["N"]},{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Hispanic Americans"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PX"],"qualifierName":["psychology"],"majorTopic_YN":["Y"]},{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2937152?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2937152"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=19915965&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=19915965"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s10964-009-9475-z"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2011-01-12"],"dateOfCreation":["2010-09-13"],"dateOfRevision":["2013-05-31"],"electronicPublicationDate":["2009-11-14"],"firstPublicationDate":["2009-11-14"],"cprojectID":"PMC2937152"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSghOUS2orL1IOA","_score":1,"_source":{"id":["23418179"],"source":["MED"],"pmid":["23418179"],"pmcid":["PMC3622304"],"doi":["10.1093/gbe/evt026"],"title":["Rapid evolutionary dynamics of structural disorder as a potential driving force for biological divergence in flaviviruses."],"authorString":["Ortiz JF, MacDonald ML, Masterson P, Uversky VN, Siltberg-Liberles J."],"authorList":[{"author":[{"fullName":["Ortiz JF"],"firstName":["Juan F"],"lastName":["Ortiz"],"initials":["JF"]},{"fullName":["MacDonald ML"],"firstName":["Madolyn L"],"lastName":["MacDonald"],"initials":["ML"]},{"fullName":["Masterson P"],"firstName":["Patrick"],"lastName":["Masterson"],"initials":["P"]},{"fullName":["Uversky VN"],"firstName":["Vladimir N"],"lastName":["Uversky"],"initials":["VN"]},{"fullName":["Siltberg-Liberles J"],"firstName":["Jessica"],"lastName":["Siltberg-Liberles"],"initials":["J"]}]}],"journalInfo":[{"issue":["3"],"volume":["5"],"journalIssueId":["2048816"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Genome biology and evolution"],"ISOAbbreviation":["Genome Biol Evol"],"medlineAbbreviation":["Genome Biol Evol"],"NLMid":["101509707"],"ESSN":["1759-6653"]}]}],"pubYear":["2013"],"pageInfo":["504-513"],"abstractText":["Protein structure is commonly regarded to be conserved and to dictate function. Most proteins rely on conformational flexibility to some degree. Are regions that convey conformational flexibility conserved over evolutionary time? Can changes in conformational flexibility alter protein function? Here, the evolutionary dynamics of structurally ordered and disordered (flexible) regions are investigated genome-wide in flaviviruses, revealing that the amount and location of structural disorder fluctuates highly among related proteins. Some regions are prone to shift between structured and flexible states. Increased evolutionary dynamics of structural disorder is observed for some lineages but not in others. Lineage-specific transitions of this kind could alter the conformational ensemble accessible to the same protein in different species, causing a functional change, even if the predominant function remains conserved. Thus, rapid evolutionary dynamics of structural disorder is a potential driving force for phenotypic divergence among flaviviruses."],"affiliation":["Department of Molecular Biology, University of Wyoming, USA."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["8 P20 GM103432-12"],"agency":["NIGMS NIH HHS"],"acronym":["GM"],"orderIn":["0"]},{"grantId":["5P20RR016474-12"],"agency":["NCRR NIH HHS"],"acronym":["RR"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Alignment"]},{"majorTopic_YN":["Y"],"descriptorName":["Evolution, Molecular"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acid Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Biological Evolution"]},{"majorTopic_YN":["N"],"descriptorName":["Mutation Rate"]}]}],"chemicalList":[{"chemical":[{"name":["Viral Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3622304?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3622304"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23418179"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23418179&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/gbe/evt026"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["6"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-08-14"],"dateOfCreation":["2013-03-13"],"dateOfRevision":["2013-10-24"],"firstPublicationDate":["2013-01-01"],"cprojectID":"PMC3622304"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSW_8VqgcuJwvfw","_score":1,"_source":{"id":["16485468"],"source":["MED"],"pmid":["16485468"],"pmcid":["PMC3367654"],"doi":["10.3201/eid1112.050997"],"title":["Host range and emerging and reemerging pathogens."],"authorString":["Woolhouse ME, Gowtage-Sequeria S."],"authorList":[{"author":[{"fullName":["Woolhouse ME"],"firstName":["Mark E J"],"lastName":["Woolhouse"],"initials":["ME"]},{"fullName":["Gowtage-Sequeria S"],"firstName":["Sonya"],"lastName":["Gowtage-Sequeria"],"initials":["S"]}]}],"journalInfo":[{"issue":["12"],"volume":["11"],"journalIssueId":["1298338"],"dateOfPublication":["2005 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2005"],"printPublicationDate":["2005-12-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2005"],"pageInfo":["1842-1847"],"abstractText":["An updated literature survey identified 1,407 recognized species of human pathogen, 58% of which are zoonotic. Of the total, 177 are regarded as emerging or reemerging. Zoonotic pathogens are twice as likely to be in this category as are nonzoonotic pathogens. Emerging and reemerging pathogens are not strongly associated with particular types of nonhuman hosts, but they are most likely to have the broadest host ranges. Emerging and reemerging zoonoses are associated with a wide range of drivers, but changes in land use and agriculture and demographic and societal changes are most commonly cited. However, although zoonotic pathogens do represent the most likely source of emerging and reemerging infectious disease, only a small minority have proved capable of causing major epidemics in the human population."],"affiliation":["Centre for Infectious Diseases, University of Edinburgh, Edinburgh, United Kingdom. mark.woolhouse@ed.ac.uk"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Helminths"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Bacteria"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Fungi"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zoonoses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["N"]},{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Reservoirs"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["N"]},{"abbreviation":["VE"],"qualifierName":["veterinary"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Demography"]},{"majorTopic_YN":["N"],"descriptorName":["Eukaryota"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3367654?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3367654"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1112.050997"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["237"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2006-03-16"],"dateOfCreation":["2006-02-20"],"dateOfRevision":["2012-09-19"],"firstPublicationDate":["2005-12-01"],"cprojectID":"PMC3367654"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSV_8VqgcuJwvfv","_score":1,"_source":{"id":["22514548"],"source":["MED"],"pmid":["22514548"],"pmcid":["PMC3323869"],"doi":["10.3389/fmicb.2012.00124"],"title":["The influence of extracellular superoxide on iron redox chemistry and bioavailability to aquatic microorganisms."],"authorString":["Rose AL."],"authorList":[{"author":[{"fullName":["Rose AL"],"firstName":["Andrew L"],"lastName":["Rose"],"initials":["AL"]}]}],"journalInfo":[{"volume":["3"],"journalIssueId":["1899249"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2012"],"pageInfo":["124"],"abstractText":["Superoxide, the one-electron reduced form of dioxygen, is produced in the extracellular milieu of aquatic microbes through a range of abiotic chemical processes and also by microbes themselves. Due to its ability to promote both oxidative and reductive reactions, superoxide may have a profound impact on the redox state of iron, potentially influencing iron solubility, complex speciation, and bioavailability. The interplay between iron, superoxide, and oxygen may also produce a cascade of other highly reactive transients in oxygenated natural waters. For microbes, the overall effect of reactions between superoxide and iron may be deleterious or beneficial, depending on the organism and its chemical environment. Here I critically discuss recent advances in understanding: (i) sources of extracellular superoxide in natural waters, with a particular emphasis on microbial generation; (ii) the chemistry of reactions between superoxide and iron; and (iii) the influence of these processes on iron bioavailability and microbial iron nutrition."],"affiliation":["Southern Cross GeoScience, Southern Cross University Lismore, NSW, Australia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Iron","Superoxide","Bioavailability"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3323869?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3323869"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22514548"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22514548&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2012.00124"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["6"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2012-10-02"],"dateOfCreation":["2012-04-19"],"dateOfRevision":["2013-08-13"],"electronicPublicationDate":["2012-04-11"],"firstPublicationDate":["2012-04-11"],"cprojectID":"PMC3323869"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSc_8VqgcuJwvf0","_score":1,"_source":{"id":["23809208"],"source":["MED"],"pmid":["23809208"],"pmcid":["PMC3765938"],"doi":["10.1186/1743-422x-10-201"],"title":["A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches."],"authorString":["Carter JR, Balaraman V, Kucharski CA, Fraser TS, Fraser MJ."],"authorList":[{"author":[{"fullName":["Carter JR"],"firstName":["James R"],"lastName":["Carter"],"initials":["JR"],"affiliation":["Department of Biological Sciences, Eck Institute of Global Health, University of Notre Dame, Notre Dame, IN 46556, USA. jcarte11@nd.edu."]},{"fullName":["Balaraman V"],"firstName":["Velmurugan"],"lastName":["Balaraman"],"initials":["V"]},{"fullName":["Kucharski CA"],"firstName":["Cheryl A"],"lastName":["Kucharski"],"initials":["CA"]},{"fullName":["Fraser TS"],"firstName":["Tresa S"],"lastName":["Fraser"],"initials":["TS"]},{"fullName":["Fraser MJ Jr"],"firstName":["Malcolm J"],"lastName":["Fraser"],"initials":["MJ"]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2002717"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2013"],"pageInfo":["201"],"abstractText":["BACKGROUND: Recent epidemics of dengue viruses (DENV) coupled with new outbreaks on the horizon have renewed the demand for novel detection methods that have the ability to identify this viral pathogen prior to the manifestation of symptoms. The ability to detect DENV in a timely manner is essential for rapid recovery from disease symptoms. A modified lab-derived 10-23 DNAzyme tethered to gold nanoparticles provides a powerful tool for the detection of viruses, such as DENV. RESULTS: We examined the effectiveness of coupling DNAzyme (DDZ) activation to the salt-induced aggregation of gold nanoparticles (AuNP) to detect dengue virus (DENV) progeny in mosquito cells. A DNAzyme was designed to recognize the 5' cyclization sequence (5' CS) that is conserved among all DENV, and conjugated to AuNPs. DDZ-AuNP has demonstrated the ability to detect the genomic RNA of our model dengue strain, DENV-2 NGC, isolated from infected Aedes albopictus C6/36 cells. These targeting events lead to the rapid aggregation of AuNPs, resulting in a red to clear color transition of the reaction mixes, and thus positive detection of the DENV RNA genome. The inclusion of SDS in the reaction mixture permitted the detection of DENV directly from cell culture supernatants without additional sample processing. Specificity assays demonstrated detection is DENV-specific, while sensitivity assays confirm detection at levels of 1 × 10(1) TCID50 units. These results demonstrate DDZ-AuNP effectively detects DENV genomes in a sequence specific manner and at concentrations that are practical for field use. CONCLUSIONS: We have developed an effective detection assay using DNAzyme catalysis coupled with AuNP aggregation for the detection of DENV genomes in a sequence specific manner. Full development of our novel DDZ-AuNP detection method will provide a practical, rapid, and low cost alternative for the detection of DENV in mosquito cells and tissues, and possibly infected patient serum, in a matter of minutes with little to no specialized training required."],"affiliation":["Department of Biological Sciences, Eck Institute of Global Health, University of Notre Dame, Notre Dame, IN 46556, USA. jcarte11@nd.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R01AI048561"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["R01 AI097554"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Cell Line"]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Gold"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DU"],"qualifierName":["diagnostic use"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Catalytic"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DU"],"qualifierName":["diagnostic use"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Virology"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Nanoparticles"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DU"],"qualifierName":["diagnostic use"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Gold"],"registryNumber":["7440-57-5"]},{"name":["RNA, Viral"],"registryNumber":["0"]},{"name":["DNA, Catalytic"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3765938?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3765938"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23809208&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23809208"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1743-422X-10-201"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2014-06-23"],"dateOfCreation":["2013-11-15"],"electronicPublicationDate":["2013-06-28"],"firstPublicationDate":["2013-06-28"],"cprojectID":"PMC3765938"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqm_J9hGurOzZIYO","_score":1,"_source":{"id":["25859151"],"source":["MED"],"pmid":["25859151"],"pmcid":["PMC4361343"],"doi":["10.2149/tmh.2014-17"],"title":["Assessment of Climate-sensitive Infectious Diseases in the Federated States of Micronesia."],"authorString":["McIver L, Hashizume M, Kim H, Honda Y, Pretrick M, Iddings S, Pavlin B."],"authorList":[{"author":[{"fullName":["McIver L"],"firstName":["Lachlan"],"lastName":["McIver"],"initials":["L"],"affiliation":["National Centre for Epidemiology and Population Health, Australian National University , Canberra, Australia."]},{"fullName":["Hashizume M"],"firstName":["Masahiro"],"lastName":["Hashizume"],"initials":["M"],"affiliation":["Institute of Tropical Medicine, Nagasaki University , 1-12-4 Sakamoto, Nagasaki 852-8523 Japan."]},{"fullName":["Kim H"],"firstName":["Ho"],"lastName":["Kim"],"initials":["H"],"affiliation":["Graduate School of Public Health, Seoul National University , Building 221, 1 Gwanak-ro, Gwanak-gu, Seoul, Korea 151-742."]},{"fullName":["Honda Y"],"firstName":["Yasushi"],"lastName":["Honda"],"initials":["Y"],"affiliation":["University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-0006, Japan."]},{"fullName":["Pretrick M"],"firstName":["Moses"],"lastName":["Pretrick"],"initials":["M"],"affiliation":["Department of Health and Social Affairs , P.O. Box PS 70 Palikir, Pohnpei 96941, Federated States of Micronesia."]},{"fullName":["Iddings S"],"firstName":["Steven"],"lastName":["Iddings"],"initials":["S"],"affiliation":["World Health Organization , Sankat Chak Tomouk, Khan Daun Penh, Phnom Penh, Cambodia."]},{"fullName":["Pavlin B"],"firstName":["Boris"],"lastName":["Pavlin"],"initials":["B"],"affiliation":["World Health Organization , 4th Floor, Aopi Centre, Waigani Drive, Port Moresby, Papua New Guinea."]}]}],"journalInfo":[{"issue":["1"],"volume":["43"],"journalIssueId":["2258584"],"dateOfPublication":["2015 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2015"],"printPublicationDate":["2015-03-01"],"journal":[{"title":["Tropical medicine and health"],"ISOAbbreviation":["Trop Med Health"],"medlineAbbreviation":["Trop Med Health"],"NLMid":["101215093"],"ISSN":["1348-8945"],"ESSN":["1349-4147"]}]}],"pubYear":["2015"],"pageInfo":["29-40"],"abstractText":["BACKGROUND: The health impacts of climate change are an issue of growing concern in the Pacific region. Prior to 2010, no formal, structured, evidence-based approach had been used to identify the most significant health risks posed by climate change in Pacific island countries. During 2010 and 2011, the World Health Organization supported the Federated States of Micronesia (FSM) in performing a climate change and health vulnerability and adaptation assessment. This paper summarizes the priority climate-sensitive health risks in FSM, with a focus on diarrheal disease, its link with climatic variables and the implications of climate change. METHODS: The vulnerability and adaptation assessment process included a review of the literature, extensive stakeholder consultations, ranking of climate-sensitive health risks, and analysis of the available long-term data on climate and climate-sensitive infectious diseases in FSM, which involved examination of health information data from the four state hospitals in FSM between 2000 and 2010; along with each state's rainfall, temperature and El Niño-Southern Oscillation data. Generalized linear Poisson regression models were used to demonstrate associations between monthly climate variables and cases of climate-sensitive diseases at differing temporal lags. RESULTS: Infectious diseases were among the highest priority climate-sensitive health risks identified in FSM, particularly diarrheal diseases, vector-borne diseases and leptospirosis. Correlation with climate data demonstrated significant associations between monthly maximum temperature and monthly outpatient cases of diarrheal disease in Pohnpei and Kosrae at a lag of one month and 0 to 3 months, respectively; no such associations were observed in Chuuk or Yap. Significant correlations between disease incidence and El Niño-Southern Oscillation cycles were demonstrated in Kosrae state. CONCLUSIONS: Analysis of the available data demonstrated significant associations between climate variables and climate-sensitive infectious diseases. This information should prove useful in implementing health system and community adaptation strategies to avoid the most serious impacts of climate change on health in FSM."],"affiliation":["National Centre for Epidemiology and Population Health, Australian National University , Canberra, Australia."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Infectious diseases","Climate","Federated States Of Micronesia"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4361343?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4361343"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2149/tmh.2014-17"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-04-10"],"dateOfCreation":["2015-04-10"],"dateOfRevision":["2015-04-12"],"electronicPublicationDate":["2014-11-15"],"firstPublicationDate":["2014-11-15"],"cprojectID":"PMC4361343"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoEhOUS2orL1IOc","_score":1,"_source":{"id":["25589229"],"source":["MED"],"pmid":["25589229"],"pmcid":["PMC4295104"],"doi":["10.1038/srep07795"],"title":["Functional characterization of aquaporins and aquaglyceroporins of the yellow fever mosquito, Aedes aegypti."],"authorString":["Drake LL, Rodriguez SD, Hansen IA."],"authorList":[{"author":[{"fullName":["Drake LL"],"firstName":["Lisa L"],"lastName":["Drake"],"initials":["LL"],"affiliation":["Department of Biology, New Mexico State University, Las Cruces, NM."]},{"fullName":["Rodriguez SD"],"firstName":["Stacy D"],"lastName":["Rodriguez"],"initials":["SD"],"affiliation":["Department of Biology, New Mexico State University, Las Cruces, NM."]},{"fullName":["Hansen IA"],"firstName":["Immo A"],"lastName":["Hansen"],"initials":["IA"],"affiliation":["1] Department of Biology, New Mexico State University, Las Cruces, NM [2] Institute for Applied Biosciences, New Mexico State University, Las Cruces, NM [3] Molecular Biology Program, New Mexico State University, Las Cruces, NM."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2243512"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2015"],"pageInfo":["7795"],"abstractText":["After taking vertebrate blood, female mosquitoes quickly shed excess water and ions while retaining and concentrating the mostly proteinaceous nutrients. Aquaporins (AQPs) are an evolutionary conserved family of membrane transporter proteins that regulate the flow of water and in some cases glycerol and other small molecules across cellular membranes. In a previous study, we found six putative AQP genes in the genome of the yellow fever mosquito, Ae. aegypti, and demonstrated the involvement of three of them in the blood meal-induced diuresis. Here we characterized AQP expression in different tissues before and after a blood meal, explored the substrate specificity of AQPs expressed in the Malpighian tubules and performed RNAi-mediated knockdown and tested for changes in mosquito desiccation resistance. We found that AQPs are generally down-regulated 24 hrs after a blood meal. Ae. aegypti AQP 1 strictly transports water, AQP 2 and 5 demonstrate limited solute transport, but primarily function as water transporters. AQP 4 is an aquaglyceroporin with multiple substrates. Knockdown of AQPs expressed in the MTs increased survival of Ae. aegypti under dry conditions. We conclude that Malpighian tubules of adult female yellow fever mosquitoes utilize three distinct AQPs and one aquaglyceroporin in their osmoregulatory functions."],"affiliation":["Department of Biology, New Mexico State University, Las Cruces, NM."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Oocytes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Xenopus"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Water"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Aquaporins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Biological Assay"]},{"majorTopic_YN":["N"],"descriptorName":["Desiccation"]},{"majorTopic_YN":["N"],"descriptorName":["Gene Expression Profiling"]},{"majorTopic_YN":["N"],"descriptorName":["Cell Membrane Permeability"]},{"majorTopic_YN":["N"],"descriptorName":["Organ Specificity"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Gene Expression Regulation"]},{"majorTopic_YN":["N"],"descriptorName":["RNA Interference"]},{"majorTopic_YN":["N"],"descriptorName":["Biological Transport"]},{"majorTopic_YN":["N"],"descriptorName":["Aquaglyceroporins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Gene Knockdown Techniques"]}]}],"chemicalList":[{"chemical":[{"name":["Insect Proteins"],"registryNumber":["0"]},{"name":["Aquaporins"],"registryNumber":["0"]},{"name":["Aquaglyceroporins"],"registryNumber":["0"]},{"name":["Water"],"registryNumber":["059QF0KO0R"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4295104?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4295104"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep07795"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-10-13"],"dateOfCreation":["2015-01-15"],"dateOfRevision":["2015-01-28"],"electronicPublicationDate":["2015-01-15"],"firstPublicationDate":["2015-01-15"],"cprojectID":"PMC4295104"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoihOUS2orL1IOh","_score":1,"_source":{"id":["25811410"],"source":["MED"],"pmid":["25811410"],"pmcid":["PMC4378478"],"doi":["10.3201/eid2104.141707"],"title":["Zika virus infection, Philippines, 2012."],"authorString":["Alera MT, Hermann L, Tac-An IA, Klungthong C, Rutvisuttinunt W, Manasatienkij W, Villa D, Thaisomboonsuk B, Velasco JM, Chinnawirotpisan P, Lago CB, Roque VG, Macareo LR, Srikiatkhachorn A, Fernandez S, Yoon IK."],"authorList":[{"author":[{"fullName":["Alera MT"],"firstName":["Maria Theresa"],"lastName":["Alera"],"initials":["MT"]},{"fullName":["Hermann L"],"firstName":["Laura"],"lastName":["Hermann"],"initials":["L"]},{"fullName":["Tac-An IA"],"firstName":["Ilya A"],"lastName":["Tac-An"],"initials":["IA"]},{"fullName":["Klungthong C"],"firstName":["Chonticha"],"lastName":["Klungthong"],"initials":["C"]},{"fullName":["Rutvisuttinunt W"],"firstName":["Wiriya"],"lastName":["Rutvisuttinunt"],"initials":["W"]},{"fullName":["Manasatienkij W"],"firstName":["Wudtichai"],"lastName":["Manasatienkij"],"initials":["W"]},{"fullName":["Villa D"],"firstName":["Daisy"],"lastName":["Villa"],"initials":["D"]},{"fullName":["Thaisomboonsuk B"],"firstName":["Butsaya"],"lastName":["Thaisomboonsuk"],"initials":["B"]},{"fullName":["Velasco JM"],"firstName":["John Mark"],"lastName":["Velasco"],"initials":["JM"]},{"fullName":["Chinnawirotpisan P"],"firstName":["Piyawan"],"lastName":["Chinnawirotpisan"],"initials":["P"]},{"fullName":["Lago CB"],"firstName":["Catherine B"],"lastName":["Lago"],"initials":["CB"]},{"fullName":["Roque VG Jr"],"firstName":["Vito G"],"lastName":["Roque"],"initials":["VG"]},{"fullName":["Macareo LR"],"firstName":["Louis R"],"lastName":["Macareo"],"initials":["LR"]},{"fullName":["Srikiatkhachorn A"],"firstName":["Anon"],"lastName":["Srikiatkhachorn"],"initials":["A"]},{"fullName":["Fernandez S"],"firstName":["Stefan"],"lastName":["Fernandez"],"initials":["S"]},{"fullName":["Yoon IK"],"firstName":["In-Kyu"],"lastName":["Yoon"],"initials":["IK"]}]}],"journalInfo":[{"issue":["4"],"volume":["21"],"journalIssueId":["2266692"],"dateOfPublication":["2015 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2015"],"printPublicationDate":["2015-04-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqolhOUS2orL1IOi","_score":1,"_source":{"id":["26038768"],"source":["MED"],"pmid":["26038768"],"pmcid":["PMC4395659"],"doi":["10.1038/emi.2015.18"],"title":["Factors responsible for the emergence of arboviruses; strategies, challenges and limitations for their control."],"authorString":["Liang G, Gao X, Gould EA."],"authorList":[{"author":[{"fullName":["Liang G"],"firstName":["Guodong"],"lastName":["Liang"],"initials":["G"],"affiliation":["State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention , Beijing 102206, China ; Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases , Hangzhou 310003, Zhejiang province, China."]},{"fullName":["Gao X"],"firstName":["Xiaoyan"],"lastName":["Gao"],"initials":["X"],"affiliation":["State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention , Beijing 102206, China ; Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases , Hangzhou 310003, Zhejiang province, China."]},{"fullName":["Gould EA"],"firstName":["Ernest A"],"lastName":["Gould"],"initials":["EA"],"affiliation":["Aix Marseille University, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 \"Emergence des Pathologies Virales\" , Marseille 13385, France ; Centre for Ecology & Hydrology , Oxford OX1 3SR, United Kingdom."]}]}],"journalInfo":[{"issue":["3"],"volume":["4"],"journalIssueId":["2290265"],"dateOfPublication":["2015 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2015"],"printPublicationDate":["2015-03-01"],"journal":[{"title":["Emerging microbes & infections"],"ISOAbbreviation":["Emerg Microbes Infect"],"medlineAbbreviation":["Emerg Microbes Infect"],"NLMid":["101594885"],"ESSN":["2222-1751"]}]}],"pubYear":["2015"],"pageInfo":["e18"],"abstractText":["Slave trading of Africans to the Americas, during the 16th to the 19th century was responsible for the first recorded emergence in the New World of two arthropod-borne viruses (arboviruses), yellow fever virus and dengue virus. Many other arboviruses have since emerged from their sylvatic reservoirs and dispersed globally due to evolving factors that include anthropological behaviour, commercial transportation and land-remediation. Here, we outline some characteristics of these highly divergent arboviruses, including the variety of life cycles they have developed and the mechanisms by which they have adapted to evolving changes in habitat and host availability. We cite recent examples of virus emergence that exemplify how arboviruses have exploited the consequences of the modern human lifestyle. Using our current understanding of these viruses, we also attempt to demonstrate some of the limitations encountered in developing control strategies to reduce the impact of future emerging arbovirus diseases. Finally, we present recommendations for development by an international panel of experts reporting directly to World Health Organization, with the intention of providing internationally acceptable guidelines for improving emerging arbovirus disease control strategies. Success in these aims should alleviate the suffering and costs encountered during recent decades when arboviruses have emerged from their sylvatic environment."],"affiliation":["State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention , Beijing 102206, China ; Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases , Hangzhou 310003, Zhejiang province, China."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Arboviruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Arbovirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]}]}]}]}],"keywordList":[{"keyword":["emergence","Arbovirus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4395659?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4395659"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/emi.2015.18"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-25"],"dateOfCreation":["2015-06-03"],"electronicPublicationDate":["2015-03-25"],"firstPublicationDate":["2015-03-25"],"cprojectID":"PMC4395659"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqrS_8VqgcuJwvgX","_score":1,"_source":{"id":["25421891"],"source":["MED"],"pmid":["25421891"],"pmcid":["PMC4246241"],"doi":["10.3390/v6114628"],"title":["Chikungunya virus-vector interactions."],"authorString":["Coffey LL, Failloux AB, Weaver SC."],"authorList":[{"author":[{"fullName":["Coffey LL"],"firstName":["Lark L"],"lastName":["Coffey"],"initials":["LL"],"affiliation":["Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. lcoffey@ucdavis.edu."]},{"fullName":["Failloux AB"],"firstName":["Anna-Bella"],"lastName":["Failloux"],"initials":["AB"],"affiliation":["Department of Virology, Arboviruses and Insect Vectors, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris cedex 15, France. anna-bella.failloux@pasteur.fr."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Institute for Human Infections and Immunity, Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA. sweaver@utmb.edu."]}]}],"journalInfo":[{"issue":["11"],"volume":["6"],"journalIssueId":["2236404"],"dateOfPublication":["2014 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2014"],"printPublicationDate":["2014-11-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2014"],"pageInfo":["4628-4663"],"abstractText":["Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes chikungunya fever, a severe, debilitating disease that often produces chronic arthralgia. Since 2004, CHIKV has emerged in Africa, Indian Ocean islands, Asia, Europe, and the Americas, causing millions of human infections. Central to understanding CHIKV emergence is knowledge of the natural ecology of transmission and vector infection dynamics. This review presents current understanding of CHIKV infection dynamics in mosquito vectors and its relationship to human disease emergence. The following topics are reviewed: CHIKV infection and vector life history traits including transmission cycles, genetic origins, distribution, emergence and spread, dispersal, vector competence, vector immunity and microbial interactions, and co-infection by CHIKV and other arboviruses. The genetics of vector susceptibility and host range changes, population heterogeneity and selection for the fittest viral genomes, dual host cycling and its impact on CHIKV adaptation, viral bottlenecks and intrahost diversity, and adaptive constraints on CHIKV evolution are also discussed. The potential for CHIKV re-emergence and expansion into new areas and prospects for prevention via vector control are also briefly reviewed."],"affiliation":["Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. lcoffey@ucdavis.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Host-Pathogen Interactions"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4246241?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4246241"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v6114628"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["10"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-10-19"],"dateOfCreation":["2014-11-25"],"dateOfRevision":["2014-12-24"],"electronicPublicationDate":["2014-11-24"],"firstPublicationDate":["2014-11-24"],"cprojectID":"PMC4246241"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsBJ9hGurOzZIYY","_score":1,"_source":{"id":["26225214"],"source":["MED"],"pmid":["26225214"],"pmcid":["PMC4518591"],"doi":["10.1186/s40201-015-0211-1"],"title":["Determination of hydrogen cyanide concentration in mainstream smoke of tobacco products by polarography."],"authorString":["Mahernia S, Amanlou A, Kiaee G, Amanlou M."],"authorList":[{"author":[{"fullName":["Mahernia S"],"firstName":["Shabnam"],"lastName":["Mahernia"],"initials":["S"],"affiliation":["Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran."]},{"fullName":["Amanlou A"],"firstName":["Arash"],"lastName":["Amanlou"],"initials":["A"],"affiliation":["Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran."]},{"fullName":["Kiaee G"],"firstName":["Gita"],"lastName":["Kiaee"],"initials":["G"],"affiliation":["Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran."]},{"fullName":["Amanlou M"],"firstName":["Massoud"],"lastName":["Amanlou"],"initials":["M"],"affiliation":["Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2250104"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Journal of environmental health science & engineering"],"ISOAbbreviation":["J Environ Health Sci Eng"],"medlineAbbreviation":["J Environ Health Sci Eng"],"NLMid":["101613643"],"ESSN":["2052-336X"]}]}],"pubYear":["2015"],"pageInfo":["57"],"abstractText":["BACKGROUND: There has been a worldwide concern for the health risks of cigarette smoking and hydrogen cyanide (HCN) considered as one of the hazardous tobacco compounds which is needed to be determined in order to reduce the dose related to smoke disease risk. In this study, we prepare the experimental procedure to entrap the HCN from mainstream smoke of different brands of Tehran cigarette, through simulating human inhalation and determine its concentration applying polarography. RESULTS: The HCN level of the 50 commonly consumed tobacco products (47 cigarettes and 3 cigars) obtained from local store is ranged between 17.56 ± 1.02 and 1553.98 ± 0.56 μg per stick, this acquired amount is more than FDA approval (10 μg per stick), so the harmful effects of smoking is indicative. CONCLUSIONS: The comparative study of the results shows that the price and the weight of each product do not indicate HCN level. As can be seen, R(2) value which is a statistical measure of how close the data are to the fitted regression line is low (R(2) < 0.2). So it should not be deceived by names such as ultra light or infinite gravity to suck, because this names or the price haven(')t effect on the amount of HCN and its destructive effects."],"affiliation":["Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Polarography","Cigarette","Hydrogen Cyanide","Tobacco Products"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4518591?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4518591"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40201-015-0211-1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-07-30"],"dateOfCreation":["2015-07-30"],"dateOfRevision":["2015-08-02"],"electronicPublicationDate":["2015-07-29"],"firstPublicationDate":["2015-07-29"],"cprojectID":"PMC4518591"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqxx_8VqgcuJwvgp","_score":1,"_source":{"id":["25880767"],"source":["MED"],"pmid":["25880767"],"pmcid":["PMC4400050"],"doi":["10.1371/journal.pntd.0003575"],"title":["Neglected tropical diseases among the Association of Southeast Asian Nations (ASEAN): overview and update."],"authorString":["Hotez PJ, Bottazzi ME, Strych U, Chang LY, Lim YA, Goodenow MM, AbuBakar S."],"authorList":[{"author":[{"fullName":["Hotez PJ"],"firstName":["Peter J"],"lastName":["Hotez"],"initials":["PJ"],"authorId":[{"_":"0000-0001-8770-1042","$":{"type":"ORCID"}}],"affiliation":["Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development, Departments of Pediatrics and Molecular Virology and Microbiology, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America; Department of Biology, Baylor University, Waco, Texas, United States of America; James A. Baker III Institute for Public Policy, Rice University, Houston, Texas, United States of America."]},{"fullName":["Bottazzi ME"],"firstName":["Maria Elena"],"lastName":["Bottazzi"],"initials":["ME"],"affiliation":["Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development, Departments of Pediatrics and Molecular Virology and Microbiology, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America; Department of Biology, Baylor University, Waco, Texas, United States of America."]},{"fullName":["Strych U"],"firstName":["Ulrich"],"lastName":["Strych"],"initials":["U"],"authorId":[{"_":"0000-0001-9455-7683","$":{"type":"ORCID"}}],"affiliation":["Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development, Departments of Pediatrics and Molecular Virology and Microbiology, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America."]},{"fullName":["Chang LY"],"firstName":["Li-Yen"],"lastName":["Chang"],"initials":["LY"],"affiliation":["Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia."]},{"fullName":["Lim YA"],"firstName":["Yvonne A L"],"lastName":["Lim"],"initials":["YA"],"authorId":[{"_":"0000-0003-4050-6332","$":{"type":"ORCID"}}],"affiliation":["Department of Parasitology, University of Malaya, Kuala Lumpur, Malaysia."]},{"fullName":["Goodenow MM"],"firstName":["Maureen M"],"lastName":["Goodenow"],"initials":["MM"],"affiliation":["Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, College of Medicine, Gainesville, Florida, United States of America."]},{"fullName":["AbuBakar S"],"firstName":["Sazaly"],"lastName":["AbuBakar"],"initials":["S"],"affiliation":["Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-8770-1042","$":{"type":"ORCID"}},{"_":"0000-0001-9455-7683","$":{"type":"ORCID"}},{"_":"0000-0003-4050-6332","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["9"],"journalIssueId":["2269290"],"dateOfPublication":["2015 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2015"],"printPublicationDate":["2015-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0003575"],"abstractText":["The ten member states of the Association of Southeast Asian Nations (ASEAN) constitute an economic powerhouse, yet these countries also harbor a mostly hidden burden of poverty and neglected tropical diseases (NTDs). Almost 200 million people live in extreme poverty in ASEAN countries, mostly in the low or lower middle-income countries of Indonesia, the Philippines, Myanmar, Viet Nam, and Cambodia, and many of them are affected by at least one NTD. However, NTDs are prevalent even among upper middle-income ASEAN countries such as Malaysia and Thailand, especially among the indigenous populations. The three major intestinal helminth infections are the most common NTDs; each helminthiasis is associated with approximately 100 million infections in the region. In addition, more than 10 million people suffer from either liver or intestinal fluke infections, as well as schistosomiasis and lymphatic filariasis (LF). Intestinal protozoan infections are widespread, while leishmaniasis has emerged in Thailand, and zoonotic malaria (Plasmodium knowlesi infection) causes severe morbidity in Malaysia. Melioidosis has emerged as an important bacterial NTD, as have selected rickettsial infections, and leptospirosis. Leprosy, yaws, and trachoma are still endemic in focal areas. Almost 70 million cases of dengue fever occur annually in ASEAN countries, such that this arboviral infection is now one of the most common and economically important NTDs in the region. A number of other arboviral and zoonotic viral infections have also emerged, including Japanese encephalitis; tick-borne viral infections; Nipah virus, a zoonosis present in fruit bats; and enterovirus 71 infection. There are urgent needs to expand surveillance activities in ASEAN countries, as well as to ensure mass drug administration is provided to populations at risk for intestinal helminth and fluke infections, LF, trachoma, and yaws. An ASEAN Network for Drugs, Diagnostics, Vaccines, and Traditional Medicines Innovation provides a policy framework for the development of new control and elimination tools. Together with prominent research institutions and universities, the World Health Organization (WHO), and its regional offices, these organizations could implement important public health improvements through NTD control and elimination in the coming decade."],"affiliation":["Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development, Departments of Pediatrics and Molecular Virology and Microbiology, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, United States of America; Department of Biology, Baylor University, Waco, Texas, United States of America; James A. Baker III Institute for Public Policy, Rice University, Houston, Texas, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Tropical Medicine"]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Disease Control"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Socioeconomic Factors"]},{"majorTopic_YN":["N"],"descriptorName":["Asia, Southeastern"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Neglected Diseases"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4400050?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4400050"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0003575"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["3"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-12-17"],"dateOfCreation":["2015-04-17"],"dateOfRevision":["2015-05-22"],"electronicPublicationDate":["2015-04-16"],"firstPublicationDate":["2015-04-16"],"cprojectID":"PMC4400050"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqzVJ9hGurOzZIYs","_score":1,"_source":{"id":["26584464"],"source":["MED"],"pmid":["26584464"],"pmcid":["PMC4672408"],"doi":["10.3201/eid2112.151167"],"title":["Outbreak of Exanthematous Illness Associated with Zika, Chikungunya, and Dengue Viruses, Salvador, Brazil."],"authorString":["Cardoso CW, Paploski IA, Kikuti M, Rodrigues MS, Silva MM, Campos GS, Sardi SI, Kitron U, Reis MG, Ribeiro GS."],"authorList":[{"author":[{"fullName":["Cardoso CW"],"firstName":["Cristiane W"],"lastName":["Cardoso"],"initials":["CW"]},{"fullName":["Paploski IA"],"firstName":["Igor A D"],"lastName":["Paploski"],"initials":["IA"]},{"fullName":["Kikuti M"],"firstName":["Mariana"],"lastName":["Kikuti"],"initials":["M"]},{"fullName":["Rodrigues MS"],"firstName":["Moreno S"],"lastName":["Rodrigues"],"initials":["MS"]},{"fullName":["Silva MM"],"firstName":["Monaise M O"],"lastName":["Silva"],"initials":["MM"]},{"fullName":["Campos GS"],"firstName":["Gubio S"],"lastName":["Campos"],"initials":["GS"]},{"fullName":["Sardi SI"],"firstName":["Silvia I"],"lastName":["Sardi"],"initials":["SI"]},{"fullName":["Kitron U"],"firstName":["Uriel"],"lastName":["Kitron"],"initials":["U"]},{"fullName":["Reis MG"],"firstName":["Mitermayer G"],"lastName":["Reis"],"initials":["MG"]},{"fullName":["Ribeiro GS"],"firstName":["Guilherme S"],"lastName":["Ribeiro"],"initials":["GS"]}]}],"journalInfo":[{"issue":["12"],"volume":["21"],"journalIssueId":["2344391"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2015"],"pageInfo":["2274-2276"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]}]}],"keywordList":[{"keyword":["Viruses","Dengue virus","Arboviruses","Brazil","Chikungunya virus","epidemiology","Disease Outbreak","Flavivirus","Salvador","Zika Virus","Vectorborne Infections","Acute Exanthematous Illness"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4672408?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4672408"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2112.151167"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["10"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-25"],"dateOfCreation":["2015-11-20"],"dateOfRevision":["2015-12-09"],"firstPublicationDate":["2015-12-01"],"cprojectID":"PMC4672408"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq5lJ9hGurOzZIY5","_score":1,"_source":{"id":["PMC4773937"],"source":["PMC"],"pmcid":["PMC4773937"],"title":["In this month’s Bulletin."],"journalInfo":[{"issue":["3"],"volume":["94"],"journalIssueId":["2385453"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["157-157"],"language":["eng"],"pubModel":["Undetermined"],"pubTypeList":[{"pubType":["In Brief"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4773937"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.org/articles/PMC4773937/?tool=EBI"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-14"],"dateOfRevision":["2016-07-14"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4773937"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq5_hOUS2orL1IPE","_score":1,"_source":{"id":["26843462"],"source":["MED"],"pmid":["26843462"],"pmcid":["PMC4740769"],"doi":["10.1038/srep20122"],"title":["Inhibition of Chikungunya Virus Replication by 1-[(2-Methylbenzimidazol-1-yl) Methyl]-2-Oxo-Indolin-3-ylidene] Amino] Thiourea(MBZM-N-IBT)."],"authorString":["Mishra P, Kumar A, Mamidi P, Kumar S, Basantray I, Saswat T, Das I, Nayak TK, Chattopadhyay S, Subudhi BB, Chattopadhyay S."],"authorList":[{"author":[{"fullName":["Mishra P"],"firstName":["Priyadarsee"],"lastName":["Mishra"],"initials":["P"],"affiliation":["School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar, India."]},{"fullName":["Kumar A"],"firstName":["Abhishek"],"lastName":["Kumar"],"initials":["A"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Mamidi P"],"firstName":["Prabhudutta"],"lastName":["Mamidi"],"initials":["P"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Kumar S"],"firstName":["Sameer"],"lastName":["Kumar"],"initials":["S"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Basantray I"],"firstName":["Itishree"],"lastName":["Basantray"],"initials":["I"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Saswat T"],"firstName":["Tanuja"],"lastName":["Saswat"],"initials":["T"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Das I"],"firstName":["Indrani"],"lastName":["Das"],"initials":["I"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]},{"fullName":["Nayak TK"],"firstName":["Tapas Kumar"],"lastName":["Nayak"],"initials":["TK"],"affiliation":["School of Biological Sciences, National Institute of Science Education &Research, Bhubaneswar, India."]},{"fullName":["Chattopadhyay S"],"firstName":["Subhasis"],"lastName":["Chattopadhyay"],"initials":["S"],"affiliation":["School of Biological Sciences, National Institute of Science Education &Research, Bhubaneswar, India."]},{"fullName":["Subudhi BB"],"firstName":["Bharat Bhusan"],"lastName":["Subudhi"],"initials":["BB"],"affiliation":["School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar, India."]},{"fullName":["Chattopadhyay S"],"firstName":["Soma"],"lastName":["Chattopadhyay"],"initials":["S"],"affiliation":["Institute of Life Sciences, Bhubaneswar, India."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["20122"],"abstractText":["Chikungunya virus (CHIKV) infection is one of the most challenging human Arboviral infections with global significance and without any specific antiviral. In this investigation, 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea (MBZM-N-IBT) was synthesised as a molecular hybrid of 2-methyl benzimidazole and isatin-β-thiosemicarbazone and its anti-CHIKV property was evaluated. The release of infectious virus particles was calculated by plaque assay, expression profile of viral RNA was estimated by RT-PCR and viral protein profiles were assessed by Western blot and FACS analyses. The safety index of MBZM-N-IBT was found to be >21. The CHIKV infectious viral particle formation was abrogated around 76.02% by MBZM-N-IBT during infection in mammalian system and the viral RNA synthesis was reduced by 65.53% and 23.71% for nsP2 and E1 respectively. Surprisingly, the viral protein levels were reduced by 97% for both nsP2 and E2. In the time-of-addition experiment it abrogated viral infection at early as well as late phase of viral life cycle, which indicates about multiple mechanisms for its anti-CHIKV action. In silico analysis justified development of MBZM-N-IBT with good affinities for potential target proteins of CHIKV and related virus. With predictions of good drug-likeness property, it shows potential of a drug candidate which needs further experimental validation."],"affiliation":["School of Pharmaceutical Sciences, Siksha O Anusandhan University, Bhubaneswar, India."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4740769?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4740769"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep20122"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-04"],"dateOfRevision":["2016-02-10"],"electronicPublicationDate":["2016-02-04"],"firstPublicationDate":["2016-02-04"],"cprojectID":"PMC4740769"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq7QhOUS2orL1IPJ","_score":1,"_source":{"id":["26957861"],"source":["MED"],"pmid":["26957861"],"pmcid":["PMC4766771"],"doi":["10.4103/0975-7406.173860"],"title":["Journal of Pharmacy and Bioallied Sciences."],"authorString":["Jamshed SQ."],"authorList":[{"author":[{"fullName":["Jamshed SQ"],"firstName":["Shazia Qasim"],"lastName":["Jamshed"],"initials":["SQ"],"affiliation":["Editor, Journal of Pharmacy and Bioallied Sciences, Department of Pharmacy Practice, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang, Malaysia E-mail: editor@jpbsonline.org ; info@opubs.com."]}]}],"journalInfo":[{"issue":["1"],"volume":["8"],"journalIssueId":["2384633"],"dateOfPublication":["2016 Jan-Mar"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of pharmacy & bioallied sciences"],"ISOAbbreviation":["J Pharm Bioallied Sci"],"medlineAbbreviation":["J Pharm Bioallied Sci"],"NLMid":["101537209"],"ISSN":["0976-4879"],"ESSN":["0975-7406"]}]}],"pubYear":["2016"],"pageInfo":["1"],"affiliation":["Editor, Journal of Pharmacy and Bioallied Sciences, Department of Pharmacy Practice, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang, Malaysia E-mail: editor@jpbsonline.org ; info@opubs.com."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4766771"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0975-7406.173860"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-03-09"],"dateOfCreation":["2016-03-09"],"dateOfRevision":["2016-03-11"],"firstPublicationDate":["2016-01-01"],"cprojectID":"PMC4766771"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq7yJ9hGurOzZIY9","_score":1,"_source":{"id":["26910315"],"source":["MED"],"pmid":["26910315"],"pmcid":["PMC4775211"],"doi":["10.7554/elife.11285"],"title":["Evaluating probabilistic dengue risk forecasts from a prototype early warning system for Brazil."],"authorString":["Lowe R, Coelho CA, Barcellos C, Carvalho MS, Catão Rde C, Coelho GE, Ramalho WM, Bailey TC, Stephenson DB, Rodó X."],"authorList":[{"author":[{"fullName":["Lowe R"],"firstName":["Rachel"],"lastName":["Lowe"],"initials":["R"],"authorId":[{"_":"0000-0003-3939-7343","$":{"type":"ORCID"}}],"affiliation":["Climate Dynamics and Impacts Unit, Institut Català de Ciències del Clima, Barcelona, Spain."]},{"fullName":["Coelho CA"],"firstName":["Caio As"],"lastName":["Coelho"],"initials":["CA"],"affiliation":["Centro de Previsão de Tempo e Estudos Climáticos, Instituto Nacional de Pesquisas Espaciais, Cachoeira Paulista, Brazil."]},{"fullName":["Barcellos C"],"firstName":["Christovam"],"lastName":["Barcellos"],"initials":["C"],"authorId":[{"_":"0000-0002-1161-2753","$":{"type":"ORCID"}}],"affiliation":["Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Carvalho MS"],"firstName":["Marilia Sá"],"lastName":["Carvalho"],"initials":["MS"],"affiliation":["Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Catão Rde C"],"firstName":["Rafael De Castro"],"lastName":["Catão"],"initials":["Rde C"],"affiliation":["Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, Brazil."]},{"fullName":["Coelho GE"],"firstName":["Giovanini E"],"lastName":["Coelho"],"initials":["GE"],"affiliation":["Coordenação Geral do Programa Nacional de Controle da Dengue, Ministério da Saúde, Brasília, Brazil."]},{"fullName":["Ramalho WM"],"firstName":["Walter Massa"],"lastName":["Ramalho"],"initials":["WM"],"affiliation":["Faculdade de Ceilândia, Universidade de Brasília, Brasília, Brazil."]},{"fullName":["Bailey TC"],"firstName":["Trevor C"],"lastName":["Bailey"],"initials":["TC"],"affiliation":["Exeter Climate Systems, College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, United Kingdom."]},{"fullName":["Stephenson DB"],"firstName":["David B"],"lastName":["Stephenson"],"initials":["DB"],"affiliation":["Exeter Climate Systems, College of Engineering, Mathematics and Physical Sciences, University of Exeter, Exeter, United Kingdom."]},{"fullName":["Rodó X"],"firstName":["Xavier"],"lastName":["Rodó"],"initials":["X"],"affiliation":["Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-3939-7343","$":{"type":"ORCID"}},{"_":"0000-0002-1161-2753","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2360542"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["eLife"],"ISOAbbreviation":["Elife"],"medlineAbbreviation":["Elife"],"NLMid":["101579614"],"ESSN":["2050-084X"]}]}],"pubYear":["2016"],"abstractText":["Recently, a prototype dengue early warning system was developed to produce probabilistic forecasts of dengue risk three months ahead of the 2014 World Cup in Brazil. Here, we evaluate the categorical dengue forecasts across all microregions in Brazil, using dengue cases reported in June 2014 to validate the model. We also compare the forecast model framework to a null model, based on seasonal averages of previously observed dengue incidence. When considering the ability of the two models to predict high dengue risk across Brazil, the forecast model produced more hits and fewer missed events than the null model, with a hit rate of 57% for the forecast model compared to 33% for the null model. This early warning model framework may be useful to public health services, not only ahead of mass gatherings, but also before the peak dengue season each year, to control potentially explosive dengue epidemics."],"affiliation":["Climate Dynamics and Impacts Unit, Institut Català de Ciències del Clima, Barcelona, Spain."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Model","Microbiology","Climate","Infectious disease","Evaluation","epidemiology","Dengue","Early warning system","Global Health","None","Probabilistic"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4775211?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4775211"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7554/eLife.11285"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-25"],"dateOfRevision":["2016-03-08"],"electronicPublicationDate":["2016-02-24"],"firstPublicationDate":["2016-02-24"],"cprojectID":"PMC4775211"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9ShOUS2orL1IPN","_score":1,"_source":{"id":["26966326"],"source":["MED"],"pmid":["26966326"],"pmcid":["PMC4773940"],"doi":["10.2471/blt.16.030316"],"title":["Brazil's scientists scramble to solve the Zika puzzle."],"authorString":["Bispo A."],"authorList":[{"author":[{"fullName":["Bispo A"],"firstName":["Ana"],"lastName":["Bispo"],"initials":["A"]}]}],"journalInfo":[{"issue":["3"],"volume":["94"],"journalIssueId":["2385453"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["165-166"],"abstractText":["The World Health Organization has declared the recent leap in the number of microcephaly cases and their suspected association with Zika virus a public health emergency of international concern. Ana Bispo tells Andréia Azevedo Soares why Brazil should have some scientific answers in coming months."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Interview"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4773940?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4773940"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.030316"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-11"],"dateOfRevision":["2016-03-13"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4773940"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-p_8VqgcuJwvhB","_score":1,"_source":{"id":["26668431"],"source":["MED"],"pmid":["26668431"],"pmcid":["PMC4669736"],"doi":["10.2471/blt.15.166199"],"title":["Climate change and health in Pacific island states."],"authorString":["Kim R, Costello A, Campbell-Lendrum D."],"authorList":[{"author":[{"fullName":["Kim R"],"firstName":["Rokho"],"lastName":["Kim"],"initials":["R"],"affiliation":["Division of Pacific Technical Support, World Health Organization Regional Office for the Western Pacific, Level 4, Provident Plaza One, Downtown Boulevard, 33 Ellery Street, Suva, Fiji ."]},{"fullName":["Costello A"],"firstName":["Anthony"],"lastName":["Costello"],"initials":["A"],"affiliation":["Department of Maternal, Newborn, Child and Adolescent Health, World Health Organization, Geneva."]},{"fullName":["Campbell-Lendrum D"],"firstName":["Diarmid"],"lastName":["Campbell-Lendrum"],"initials":["D"],"affiliation":["Department of Public Health, Environment and Social Determinants of Health, World Health Organization, Geneva."]}]}],"journalInfo":[{"issue":["12"],"volume":["93"],"journalIssueId":["2351494"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2015"],"pageInfo":["819"],"affiliation":["Division of Pacific Technical Support, World Health Organization Regional Office for the Western Pacific, Level 4, Provident Plaza One, Downtown Boulevard, 33 Ellery Street, Suva, Fiji ."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4669736?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4669736"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.15.166199"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-12-15"],"firstPublicationDate":["2015-12-01"],"cprojectID":"PMC4669736"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrBsJ9hGurOzZIZJ","_score":1,"_source":{"id":["26986468"],"source":["MED"],"pmid":["26986468"],"pmcid":["PMC4795802"],"doi":["10.1371/journal.pntd.0004551"],"title":["Is Dengue Vector Control Deficient in Effectiveness or Evidence?: Systematic Review and Meta-analysis."],"authorString":["Bowman LR, Donegan S, McCall PJ."],"authorList":[{"author":[{"fullName":["Bowman LR"],"firstName":["Leigh R"],"lastName":["Bowman"],"initials":["LR"],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom."]},{"fullName":["Donegan S"],"firstName":["Sarah"],"lastName":["Donegan"],"initials":["S"],"affiliation":["Department of Biostatistics, University of Liverpool, Liverpool, United Kingdom."]},{"fullName":["McCall PJ"],"firstName":["Philip J"],"lastName":["McCall"],"initials":["PJ"],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom."]}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004551"],"abstractText":["Although a vaccine could be available as early as 2016, vector control remains the primary approach used to prevent dengue, the most common and widespread arbovirus of humans worldwide. We reviewed the evidence for effectiveness of vector control methods in reducing its transmission.Studies of any design published since 1980 were included if they evaluated method(s) targeting Aedes aegypti or Ae. albopictus for at least 3 months. Primary outcome was dengue incidence. Following Cochrane and PRISMA Group guidelines, database searches yielded 960 reports, and 41 were eligible for inclusion, with 19 providing data for meta-analysis. Study duration ranged from 5 months to 10 years. Studies evaluating multiple tools/approaches (23 records) were more common than single methods, while environmental management was the most common method (19 studies). Only 9/41 reports were randomized controlled trials (RCTs). Two out of 19 studies evaluating dengue incidence were RCTs, and neither reported any statistically significant impact. No RCTs evaluated effectiveness of insecticide space-spraying (fogging) against dengue. Based on meta-analyses, house screening significantly reduced dengue risk, OR 0.22 (95% CI 0.05-0.93, p = 0.04), as did combining community-based environmental management and water container covers, OR 0.22 (95% CI 0.15-0.32, p<0.0001). Indoor residual spraying (IRS) did not impact significantly on infection risk (OR 0.67; 95% CI 0.22-2.11; p = 0.50). Skin repellents, insecticide-treated bed nets or traps had no effect (p>0.5), but insecticide aerosols (OR 2.03; 95% CI 1.44-2.86) and mosquito coils (OR 1.44; 95% CI 1.09-1.91) were associated with higher dengue risk (p = 0.01). Although 23/41 studies examined the impact of insecticide-based tools, only 9 evaluated the insecticide susceptibility status of the target vector population during the study.This review and meta-analysis demonstrate the remarkable paucity of reliable evidence for the effectiveness of any dengue vector control method. Standardised studies of higher quality to evaluate and compare methods must be prioritised to optimise cost-effective dengue prevention."],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Meta-Analysis","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Incidence"]},{"majorTopic_YN":["N"],"descriptorName":["Mosquito Control"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Health Services Research"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4795802?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4795802"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004551"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-03-18"],"dateOfRevision":["2016-03-24"],"electronicPublicationDate":["2016-03-17"],"firstPublicationDate":["2016-03-17"],"cprojectID":"PMC4795802"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrDd_8VqgcuJwvhP","_score":1,"_source":{"id":["26911930"],"source":["MED"],"pmid":["26911930"],"pmcid":["PMC4766633"],"doi":["10.1186/s13071-016-1391-y"],"title":["Unique PFK regulatory property from some mosquito vectors of disease, and from Drosophila melanogaster."],"authorString":["Nunes RD, Romeiro NC, De Carvalho HT, Moreira JR, Sola-Penna M, Silva-Neto MA, Braz GR."],"authorList":[{"author":[{"fullName":["Nunes RD"],"firstName":["Rodrigo Dutra"],"lastName":["Nunes"],"initials":["RD"],"authorId":[{"_":"0000-0003-1346-291X","$":{"type":"ORCID"}}],"affiliation":["Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. rdnunes@bioqmed.ufrj.br."]},{"fullName":["Romeiro NC"],"firstName":["Nelilma Correia"],"lastName":["Romeiro"],"initials":["NC"],"affiliation":["NUPEM-Macaé, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Nelilma@iq.ufrj.br."]},{"fullName":["De Carvalho HT"],"firstName":["Hugo Tremonte"],"lastName":["De Carvalho"],"initials":["HT"],"affiliation":["Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Riode Janeiro, RJ, Brazil. hugo.carvalho@ime.uerj.br."]},{"fullName":["Moreira JR"],"firstName":["Jean Ribeiro"],"lastName":["Moreira"],"initials":["JR"],"affiliation":["Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Riode Janeiro, RJ, Brazil. je.ri@hotmail.com."]},{"fullName":["Sola-Penna M"],"firstName":["Mauro"],"lastName":["Sola-Penna"],"initials":["M"],"authorId":[{"_":"0000-0001-9796-6829","$":{"type":"ORCID"}}],"affiliation":["Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. maurosp@pharma.ufrj.br."]},{"fullName":["Silva-Neto MA"],"firstName":["Mário Alberto C"],"lastName":["Silva-Neto"],"initials":["MA"],"affiliation":["Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. maneto@bioqmed.ufrj.br."]},{"fullName":["Braz GR"],"firstName":["Glória Regina Cardoso"],"lastName":["Braz"],"initials":["GR"],"affiliation":["Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. gbraz@iq.ufrj.br."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-1346-291X","$":{"type":"ORCID"}},{"_":"0000-0001-9796-6829","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2401389"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["107"],"abstractText":["Arthropod-borne diseases are some of the most rapidly spreading diseases. Reducing the vector population is currently the only effective way to reduce case numbers. Central metabolic pathways are potential targets to control vector populations, but have not been well explored to this aim. The information available on energy metabolism, as a way to control lifespan and dispersion through flight of dipteran vectors, is inadequate.Phosphofructokinase (PFK) activity was measured in the presence of both of its substrates, fructose-6-phosphate (F6P) and ATP, as well as some allosteric effectors: Fructose- 2,6 - bisphosphate (F2, 6BP), citrate and AMP. Aedes aegypti phosphofructokinase sequence (AaPFK) was aligned with many other insects and also vertebrate sequences. A 3D AaPFK model was produced and docking experiments were performed with AMP and citrate.The kinetic parameters of AaPFK were determined for both substrates: F6P (V = 4.47 ± 0.15 μmol of F1, 6BP/min, K0.5 = 1.48 ± 0.22 mM) and ATP (V = 4.73 ± 0.57 μmol of F1, 6BP/min, K0.5 = 0.43 ± 0.10 mM). F2,6P was a powerful activator of AaPFK, even at low ATP concentrations. AaPFK inhibition by ATP was not enhanced by citrate, consistent with observations in other insects. After examining the sequence alignment of insect and non-insect PFKs, the hypothesis is that a modification of the citrate binding site is responsible for this unique behavior. AMP, a well-known positive effector of PFK, was not capable of reverting ATP inhibition. Aedes, Anopheles and Culex are dengue, malaria and filariasis vectors, respectively, and are shown to have this distinct characteristic in phosphofructokinase control. The alignment of several insect PFKs suggested a difference in the AMP binding site and a significant change in local charges, which introduces a highly negative charge in this part of the protein, making the binding of AMP unlikely. This hypothesis was supported by 3D modeling of PFK with AMP docking, which suggested that the AMP molecule binds in a reverse orientation due to the electrostatic environment. The present findings imply a potential new way to control PFK activity and are a unique feature of these Diptera.The present findings provide the first molecular explanation for citrate insensitivity in insect PFKs, as well as demonstrating for the first time AMP insensitivity in dipterans. It also identified a potential target for novel insecticides for the control of arthropod-borne diseases."],"affiliation":["Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, Brazil. rdnunes@bioqmed.ufrj.br."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4766633?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4766633"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1391-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCreation":["2016-02-25"],"dateOfRevision":["2016-02-27"],"electronicPublicationDate":["2016-02-25"],"firstPublicationDate":["2016-02-25"],"cprojectID":"PMC4766633"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrDiJ9hGurOzZIZR","_score":1,"_source":{"id":["27066299"],"source":["MED"],"pmid":["27066299"],"pmcid":["PMC4807952"],"doi":["10.1371/currents.outbreaks.50dfc7f46798675fc63e7d7da563da76"],"title":["On the Seasonal Occurrence and Abundance of the Zika Virus Vector Mosquito Aedes Aegypti in the Contiguous United States."],"authorString":["Monaghan AJ, Morin CW, Steinhoff DF, Wilhelmi O, Hayden M, Quattrochi DA, Reiskind M, Lloyd AL, Smith K, Schmidt CA, Scalf PE, Ernst K."],"authorList":[{"author":[{"fullName":["Monaghan AJ"],"firstName":["Andrew J"],"lastName":["Monaghan"],"initials":["AJ"]},{"fullName":["Morin CW"],"firstName":["Cory W"],"lastName":["Morin"],"initials":["CW"]},{"fullName":["Steinhoff DF"],"firstName":["Daniel F"],"lastName":["Steinhoff"],"initials":["DF"]},{"fullName":["Wilhelmi O"],"firstName":["Olga"],"lastName":["Wilhelmi"],"initials":["O"]},{"fullName":["Hayden M"],"firstName":["Mary"],"lastName":["Hayden"],"initials":["M"]},{"fullName":["Quattrochi DA"],"firstName":["Dale A"],"lastName":["Quattrochi"],"initials":["DA"]},{"fullName":["Reiskind M"],"firstName":["Michael"],"lastName":["Reiskind"],"initials":["M"]},{"fullName":["Lloyd AL"],"firstName":["Alun L"],"lastName":["Lloyd"],"initials":["AL"]},{"fullName":["Smith K"],"firstName":["Kirk"],"lastName":["Smith"],"initials":["K"]},{"fullName":["Schmidt CA"],"firstName":["Chris A"],"lastName":["Schmidt"],"initials":["CA"]},{"fullName":["Scalf PE"],"firstName":["Paige E"],"lastName":["Scalf"],"initials":["PE"]},{"fullName":["Ernst K"],"firstName":["Kacey"],"lastName":["Ernst"],"initials":["K"]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["An ongoing Zika virus pandemic in Latin America and the Caribbean has raised concerns that travel-related introduction of Zika virus could initiate local transmission in the United States (U.S.) by its primary vector, the mosquito Aedes aegypti.We employed meteorologically driven models for 2006-2015 to simulate the potential seasonal abundance of adult Aedes aegypti for fifty cities within or near the margins of its known U.S. range. Mosquito abundance results were analyzed alongside travel and socioeconomic factors that are proxies of viral introduction and vulnerability to human-vector contact. Meteorological conditions are largely unsuitable for Aedes aegypti over the U.S. during winter months (December-March), except in southern Florida and south Texas where comparatively warm conditions can sustain low-to-moderate potential mosquito abundance. Meteorological conditions are suitable for Aedes aegypti across all fifty cities during peak summer months (July-September), though the mosquito has not been documented in all cities. Simulations indicate the highest mosquito abundance occurs in the Southeast and south Texas where locally acquired cases of Aedes-transmitted viruses have been reported previously. Cities in southern Florida and south Texas are at the nexus of high seasonal suitability for Aedes aegypti and strong potential for travel-related virus introduction. Higher poverty rates in cities along the U.S.-Mexico border may correlate with factors that increase human exposure to Aedes aegypti. Our results can inform baseline risk for local Zika virus transmission in the U.S. and the optimal timing of vector control activities, and underscore the need for enhanced surveillance for Aedes mosquitoes and Aedes-transmitted viruses."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4807952"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.50dfc7f46798675fc63e7d7da563da76"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-12"],"dateOfCreation":["2016-04-12"],"dateOfRevision":["2016-04-13"],"electronicPublicationDate":["2016-03-16"],"firstPublicationDate":["2016-03-16"],"cprojectID":"PMC4807952"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrGa_8VqgcuJwvhZ","_score":1,"_source":{"id":["26939897"],"source":["MED"],"pmid":["26939897"],"pmcid":["PMC4776405"],"doi":["10.1186/s12941-016-0128-2"],"title":["The expanding spectrum of modes of transmission of Zika virus: a global concern."],"authorString":["Rodriguez-Morales AJ, Bandeira AC, Franco-Paredes C."],"authorList":[{"author":[{"fullName":["Rodriguez-Morales AJ"],"firstName":["Alfonso J"],"lastName":["Rodriguez-Morales"],"initials":["AJ"],"affiliation":["Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Bucaramanga, Santander, Colombia. ajrodriguezmmd@gmail.com."]},{"fullName":["Bandeira AC"],"firstName":["Antonio Carlos"],"lastName":["Bandeira"],"initials":["AC"],"affiliation":["Hospital Aliança and Faculdade de Tecnologia e Ciencias Medical School, Salvador, Brazil. antoniobandeira@gmail.com."]},{"fullName":["Franco-Paredes C"],"firstName":["Carlos"],"lastName":["Franco-Paredes"],"initials":["C"],"affiliation":["Hospital Infantil de México, Federico Gómez, México, DF, Mexico. cparedes@ppmh.org."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2409276"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Annals of clinical microbiology and antimicrobials"],"ISOAbbreviation":["Ann. Clin. Microbiol. Antimicrob."],"medlineAbbreviation":["Ann Clin Microbiol Antimicrob"],"NLMid":["101152152"],"ESSN":["1476-0711"]}]}],"pubYear":["2016"],"pageInfo":["13"],"affiliation":["Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Bucaramanga, Santander, Colombia. ajrodriguezmmd@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4776405?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4776405"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12941-016-0128-2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-04"],"dateOfRevision":["2016-03-06"],"electronicPublicationDate":["2016-03-03"],"firstPublicationDate":["2016-03-03"],"cprojectID":"PMC4776405"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrIz_8VqgcuJwvhe","_score":1,"_source":{"id":["27041891"],"source":["MED"],"pmid":["27041891"],"pmcid":["PMC4792037"],"doi":["10.4103/0976-237x.177096"],"title":["Tiger mosquito - generated devastation in the biosphere!!!"],"authorString":["Damle SG."],"authorList":[{"author":[{"fullName":["Damle SG"],"firstName":["S G"],"lastName":["Damle"],"initials":["SG"],"affiliation":["Editor-in-Chief, Contemporary Clinical Dentistry E-mail: journalccd@gmail.com."]}]}],"journalInfo":[{"issue":["1"],"volume":["7"],"journalIssueId":["2393466"],"dateOfPublication":["2016 Jan-Mar"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Contemporary clinical dentistry"],"ISOAbbreviation":["Contemp Clin Dent"],"medlineAbbreviation":["Contemp Clin Dent"],"NLMid":["101552967"],"ISSN":["0976-237X"],"ESSN":["0976-2361"]}]}],"pubYear":["2016"],"pageInfo":["1-2"],"affiliation":["Editor-in-Chief, Contemporary Clinical Dentistry E-mail: journalccd@gmail.com."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4792037"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0976-237X.177096"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-04"],"dateOfCreation":["2016-04-04"],"dateOfRevision":["2016-04-06"],"firstPublicationDate":["2016-01-01"],"cprojectID":"PMC4792037"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrKq_8VqgcuJwvhk","_score":1,"_source":{"id":["26991663"],"source":["MED"],"pmid":["26991663"],"pmcid":["PMC4798639"],"doi":["10.1371/journal.pntd.0004589"],"title":["The Zika Pandemic - A Perfect Storm?"],"authorString":["Russell PK."],"authorList":[{"author":[{"fullName":["Russell PK"],"firstName":["Philip K"],"lastName":["Russell"],"initials":["PK"],"affiliation":["Sabin Vaccine Institute, Washington, D.C., United States of America."]}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004589"],"affiliation":["Sabin Vaccine Institute, Washington, D.C., United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Editorial"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Microcephaly"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Pandemics"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animal Distribution"]},{"majorTopic_YN":["N"],"descriptorName":["Global Health"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4798639?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4798639"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004589"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-28"],"dateOfCreation":["2016-03-19"],"dateOfRevision":["2016-03-24"],"electronicPublicationDate":["2016-03-18"],"firstPublicationDate":["2016-03-18"],"cprojectID":"PMC4798639"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrNOhOUS2orL1IPz","_score":1,"_source":{"id":["27134732"],"source":["MED"],"pmid":["27134732"],"pmcid":["PMC4837980"],"doi":["10.12688/f1000research.8436.1"],"title":["Mapping Zika virus infection using geographical information systems in Tolima, Colombia, 2015-2016."],"authorString":["Rodriguez-Morales AJ, Galindo-Marquez ML, García-Loaiza CJ, Sabogal-Roman JA, Marin-Loaiza S, Ayala AF, Lozada-Riascos CO, Sarmiento-Ospina A, Vásquez-Serna H, Jimenez-Canizales CE, Escalera-Antezana JP."],"authorList":[{"author":[{"fullName":["Rodriguez-Morales AJ"],"firstName":["Alfonso J"],"lastName":["Rodriguez-Morales"],"initials":["AJ"],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia; Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Riohacha, Colombia; Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia."]},{"fullName":["Galindo-Marquez ML"],"firstName":["Maria Leonor"],"lastName":["Galindo-Marquez"],"initials":["ML"],"authorId":[{"_":"0000-0003-2498-0878","$":{"type":"ORCID"}}],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia."]},{"fullName":["García-Loaiza CJ"],"firstName":["Carlos Julian"],"lastName":["García-Loaiza"],"initials":["CJ"],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia."]},{"fullName":["Sabogal-Roman JA"],"firstName":["Juan Alejandro"],"lastName":["Sabogal-Roman"],"initials":["JA"],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia."]},{"fullName":["Marin-Loaiza S"],"firstName":["Santiago"],"lastName":["Marin-Loaiza"],"initials":["S"],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia."]},{"fullName":["Ayala AF"],"firstName":["Andrés Felipe"],"lastName":["Ayala"],"initials":["AF"],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia."]},{"fullName":["Lozada-Riascos CO"],"firstName":["Carlos O"],"lastName":["Lozada-Riascos"],"initials":["CO"],"affiliation":["Regional Information System, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Sarmiento-Ospina A"],"firstName":["Andrea"],"lastName":["Sarmiento-Ospina"],"initials":["A"],"affiliation":["Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia; Secretary of Health of Ibagué, Ibagué, Colombia."]},{"fullName":["Vásquez-Serna H"],"firstName":["Heriberto"],"lastName":["Vásquez-Serna"],"initials":["H"],"affiliation":["Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia; Secretary of Health of Tolima, Ibagué, Colombia."]},{"fullName":["Jimenez-Canizales CE"],"firstName":["Carlos E"],"lastName":["Jimenez-Canizales"],"initials":["CE"],"authorId":[{"_":"0000-0001-7728-1523","$":{"type":"ORCID"}}],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia; Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia; Secretary of Health of Tolima, Ibagué, Colombia."]},{"fullName":["Escalera-Antezana JP"],"firstName":["Juan Pablo"],"lastName":["Escalera-Antezana"],"initials":["JP"],"affiliation":["Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia; Tongji Hospital - Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2498-0878","$":{"type":"ORCID"}},{"_":"0000-0001-7728-1523","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["568"],"abstractText":["Geographical information systems (GIS) have been extensively used for the development of epidemiological maps of tropical diseases, however not yet specifically for Zika virus (ZIKV) infection.Surveillance case data of the ongoing epidemics of ZIKV in the Tolima department, Colombia (2015-2016) were used to estimate cumulative incidence rates (cases/100,000 pop.) to develop the first maps in the department and its municipalities, including detail for the capital, Ibagué. The GIS software used was Kosmo Desktop 3.0RC1®. Two thematic maps were developed according to municipality and communes incidence rates.Up to March 5, 2016, 4,094 cases of ZIKV were reported in Tolima, for cumulated rates of 289.9 cases/100,000 pop. (7.95% of the country). Burden of ZIKV infection has been concentrated in its east area, where municipalities have reported >500 cases/100,000 pop. These municipalities are bordered by two other departments, Cundinamarca (3,778 cases) and Huila (5,338 cases), which also have high incidences of ZIKV infection. Seven municipalities of Tolima ranged from 250-499.99 cases/100,000 pop., of this group five border with high incidence municipalities (>250), including the capital, where almost half of the reported cases of ZIKV in Tolima are concentrated.Use of GIS-based epidemiological maps helps to guide decisions for the prevention and control of diseases that represent significant issues in the region and the country, but also in emerging conditions such as ZIKV."],"affiliation":["Public Health and Infection Research Group, Universidad Tecnologica de Pereira, Pereira, Colombia; Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Riohacha, Colombia; Colombian Collaborative Network of Zika (RECOLZIKA), Pereira, Colombia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["epidemiology","Colombia","Public Health","Travelers","Latin America.","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837980?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837980"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8436.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-05-02"],"dateOfCreation":["2016-05-02"],"dateOfRevision":["2016-05-04"],"electronicPublicationDate":["2016-04-05"],"firstPublicationDate":["2016-04-05"],"cprojectID":"PMC4837980"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrNtJ9hGurOzZIZp","_score":1,"_source":{"id":["27158557"],"source":["MED"],"pmid":["27158557"],"pmcid":["PMC4844562"],"doi":["10.1371/currents.outbreaks.a2fe1b3d6d71e24ad2b5afe982824053"],"title":["Clinical and Epidemiological Characterization of Laboratory-Confirmed Autochthonous Cases of Zika Virus Disease in Mexico."],"authorString":["Jimenez Corona ME, De la Garza Barroso AL, Rodriguez Martínez JC, Luna Guzmán NI, Ruiz Matus C, Díaz Quiñonez JA, Lopez Martinez I, Kuri Morales PA."],"authorList":[{"author":[{"fullName":["Jimenez Corona ME"],"firstName":["Maria Eugenia"],"lastName":["Jimenez Corona"],"initials":["ME"],"affiliation":["General Directorate of Epidemiology, Ministry of Health, Mexico City, Mexico."]},{"fullName":["De la Garza Barroso AL"],"firstName":["Ana Lucía"],"lastName":["De la Garza Barroso"],"initials":["AL"],"affiliation":["Dirección General Adjunta de Epidemiología, Dirección General de Epidemiología, Secretaría de Salud, Mexico City, Mexico."]},{"fullName":["Rodriguez Martínez JC"],"firstName":["Jose Cruz"],"lastName":["Rodriguez Martínez"],"initials":["JC"],"affiliation":["Dirección de Vigilancia de Enfermedades Transmisibles, Dirección General de Epidemiología, Secretaría de Salud, Mexico City, Mexico."]},{"fullName":["Luna Guzmán NI"],"firstName":["Norma Irene"],"lastName":["Luna Guzmán"],"initials":["NI"],"affiliation":["Dirección General de Epidemiología, Secretaría de Salud, Mexico City, Mexico."]},{"fullName":["Ruiz Matus C"],"firstName":["Cuitláhuac"],"lastName":["Ruiz Matus"],"initials":["C"],"affiliation":["Dirección General de Epidemiología, Secretaría de Salud, Mexico City, Mexico."]},{"fullName":["Díaz Quiñonez JA"],"firstName":["José Alberto"],"lastName":["Díaz Quiñonez"],"initials":["JA"],"affiliation":["Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico; Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) \"Dr. Manuel Martínez Báez\", Mexico City, Mexico."]},{"fullName":["Lopez Martinez I"],"firstName":["Irma"],"lastName":["Lopez Martinez"],"initials":["I"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) \"Dr. Manuel Martínez Báez\", Mexico City, Mexico."]},{"fullName":["Kuri Morales PA"],"firstName":["Pablo A"],"lastName":["Kuri Morales"],"initials":["PA"],"affiliation":["Subsecretaría de Prevención y Promoción de la Salud, Secretaria de Salud, Mexico City, Mexico."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["Since 2014, autochthonous circulation of Zika virus (ZIKV) in the Americas was detected (Easter Island, Chile). In May 2015, Brazil confirmed autochthonous --transmission and in October of that year Colombia reported their first cases. Now more than 52 countries have reported cases, including Mexico. To deal with this contingency in Mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. Also, the Ministry of Health defined an Action Plan against ZIKV for the whole country.We analyzed 93 autochthonous cases of ZIKV disease identified by Epidemiological Surveillance System for Zika Virus in Mexico. All autochthonous cases confirmed by laboratory since November 25, 2015 to February 19, 2016 were included. A description of clinical and epidemiological characteristics of 93 cases of ZIKV disease are presenting and, we describe the Action Plan against this public health emergency. The distribution of cases by sex was 61 men and 32 women; mean age was 35 years old (S.D. 15, range 6-90). The main clinical features in the 93 cases were fever (96.6%), rash (93.3%), non-purulent conjunctivitis (88.8%), headache (85.4%), and myalgia (84.3%). No deaths were reported.The ZIKV epidemic poses new challenges to public health systems. The information provided for basic, clinical, and epidemiological research, in addition to the data derived from epidemiological surveillance is essential. However, there are still many unanswered questions regarding mechanisms of transmission, complications, and impact of this virus."],"affiliation":["General Directorate of Epidemiology, Ministry of Health, Mexico City, Mexico."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Surveillance","epidemiology","Arbovirus","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4844562"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.a2fe1b3d6d71e24ad2b5afe982824053"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-09"],"dateOfCreation":["2016-05-09"],"dateOfRevision":["2016-05-11"],"electronicPublicationDate":["2016-04-15"],"firstPublicationDate":["2016-04-15"],"cprojectID":"PMC4844562"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrPE_8VqgcuJwvhs","_score":1,"_source":{"id":["27066475"],"source":["MED"],"pmid":["27066475"],"pmcid":["PMC4812844"],"doi":["10.3389/fchem.2016.00005"],"title":["Species-Level Variability in Extracellular Production Rates of Reactive Oxygen Species by Diatoms."],"authorString":["Schneider RJ, Roe KL, Hansel CM, Voelker BM."],"authorList":[{"author":[{"fullName":["Schneider RJ"],"firstName":["Robin J"],"lastName":["Schneider"],"initials":["RJ"],"authorId":[{"_":"0000-0001-5496-7020","$":{"type":"ORCID"}}],"affiliation":["Department of Chemistry, St. John's University New York, NY, USA."]},{"fullName":["Roe KL"],"firstName":["Kelly L"],"lastName":["Roe"],"initials":["KL"],"affiliation":["Department of Chemistry and Geochemistry, Colorado School of Mines Golden, CO, USA."]},{"fullName":["Hansel CM"],"firstName":["Colleen M"],"lastName":["Hansel"],"initials":["CM"],"affiliation":["Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution Woods Hole, MA, USA."]},{"fullName":["Voelker BM"],"firstName":["Bettina M"],"lastName":["Voelker"],"initials":["BM"],"affiliation":["Department of Chemistry and Geochemistry, Colorado School of Mines Golden, CO, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-5496-7020","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2397310"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in chemistry"],"ISOAbbreviation":["Front Chem"],"medlineAbbreviation":["Front Chem"],"NLMid":["101627988"],"ESSN":["2296-2646"]}]}],"pubYear":["2016"],"pageInfo":["5"],"abstractText":["Biological production and decay of the reactive oxygen species (ROS) hydrogen peroxide (H2O2) and superoxide (O[Formula: see text]) likely have significant effects on the cycling of trace metals and carbon in marine systems. In this study, extracellular production rates of H2O2 and O[Formula: see text] were determined for five species of marine diatoms in the presence and absence of light. Production of both ROS was measured in parallel by suspending cells on filters and measuring the ROS downstream using chemiluminescence probes. In addition, the ability of these organisms to break down O[Formula: see text] and H2O2 was examined by measuring recovery of O[Formula: see text] and H2O2 added to the influent medium. O[Formula: see text] production rates ranged from undetectable to 7.3 × 10(-16) mol cell(-1) h(-1), while H2O2 production rates ranged from undetectable to 3.4 × 10(-16) mol cell(-1) h(-1). Results suggest that extracellular ROS production occurs through a variety of pathways even amongst organisms of the same genus. Thalassiosira spp. produced more O[Formula: see text] in light than dark, even when the organisms were killed, indicating that O[Formula: see text] is produced via a passive photochemical process on the cell surface. The ratio of H2O2 to O[Formula: see text] production rates was consistent with production of H2O2 solely through dismutation of O[Formula: see text] for T. oceanica, while T. pseudonana made much more H2O2 than O[Formula: see text]. T. weissflogii only produced H2O2 when stressed or killed. P. tricornutum cells did not make cell-associated ROS, but did secrete H2O2-producing substances into the growth medium. In all organisms, recovery rates for killed cultures (94-100% H2O2; 10-80% O[Formula: see text]) were consistently higher than those for live cultures (65-95% H2O2; 10-50% O[Formula: see text]). While recovery rates for killed cultures in H2O2 indicate that nearly all H2O2 was degraded by active cell processes, O[Formula: see text] decay appeared to occur via a combination of active and passive processes. Overall, this study shows that the rates and pathways for ROS production and decay vary greatly among diatom species, even between those that are closely related, and as a function of light conditions."],"affiliation":["Department of Chemistry, St. John's University New York, NY, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Culture","Hydrogen peroxide","Reactive oxygen species","Diatoms","Superoxide"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4812844?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4812844"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fchem.2016.00005"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-12"],"dateOfCreation":["2016-04-12"],"dateOfRevision":["2016-04-13"],"electronicPublicationDate":["2016-03-30"],"firstPublicationDate":["2016-03-30"],"cprojectID":"PMC4812844"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrPY_8VqgcuJwvht","_score":1,"_source":{"id":["26976611"],"source":["MED"],"pmid":["26976611"],"pmcid":["PMC4818755"],"doi":["10.15252/emmm.201506058"],"title":["Causal or not: applying the Bradford Hill aspects of evidence to the association between Zika virus and microcephaly."],"authorString":["Frank C, Faber M, Stark K."],"authorList":[{"author":[{"fullName":["Frank C"],"firstName":["Christina"],"lastName":["Frank"],"initials":["C"],"authorId":[{"_":"0000-0001-6776-8450","$":{"type":"ORCID"}}],"affiliation":["Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany."]},{"fullName":["Faber M"],"firstName":["Mirko"],"lastName":["Faber"],"initials":["M"],"affiliation":["Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany."]},{"fullName":["Stark K"],"firstName":["Klaus"],"lastName":["Stark"],"initials":["K"],"affiliation":["Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-6776-8450","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["8"],"journalIssueId":["2404342"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["EMBO molecular medicine"],"ISOAbbreviation":["EMBO Mol Med"],"medlineAbbreviation":["EMBO Mol Med"],"NLMid":["101487380"],"ISSN":["1757-4676"],"ESSN":["1757-4684"]}]}],"pubYear":["2016"],"pageInfo":["305-307"],"affiliation":["Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4818755?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4818755"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.15252/emmm.201506058"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-04"],"dateOfRevision":["2016-05-04"],"electronicPublicationDate":["2016-03-31"],"firstPublicationDate":["2016-03-14"],"cprojectID":"PMC4818755"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrQc_8VqgcuJwvhx","_score":1,"_source":{"id":["27123365"],"source":["MED"],"pmid":["27123365"],"pmcid":["PMC4830737"],"doi":["10.1155/2016/8258946"],"title":["International Air Travel to Ohio, USA, and the Impact on Malaria, Influenza, and Hepatitis A."],"authorString":["Brannen DE, Alhammad A, Branum M, Schmitt A."],"authorList":[{"author":[{"fullName":["Brannen DE"],"firstName":["Donald E"],"lastName":["Brannen"],"initials":["DE"],"affiliation":["Greene County Public Health, 360 Wilson Drive, Xenia, OH 45385, USA; Wright State University, Dayton, OH 45435, USA; Xavier University, Cincinnati, OH 45207, USA."]},{"fullName":["Alhammad A"],"firstName":["Ali"],"lastName":["Alhammad"],"initials":["A"],"affiliation":["Wright State University, Dayton, OH 45435, USA; Division of Aerospace Medicine, Boonshoft College of Medicine, Wright State University, Dayton, OH 45435, USA; Royal Saudi Arabian Armed Forces Medical Services, Jeddah 21577, Saudi Arabia."]},{"fullName":["Branum M"],"firstName":["Melissa"],"lastName":["Branum"],"initials":["M"],"affiliation":["Greene County Public Health, 360 Wilson Drive, Xenia, OH 45385, USA; Wright State University, Dayton, OH 45435, USA."]},{"fullName":["Schmitt A"],"firstName":["Amy"],"lastName":["Schmitt"],"initials":["A"],"affiliation":["Greene County Public Health, 360 Wilson Drive, Xenia, OH 45385, USA."]}]}],"journalInfo":[{"volume":["2016"],"journalIssueId":["2391230"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientifica"],"ISOAbbreviation":["Scientifica (Cairo)"],"medlineAbbreviation":["Scientifica (Cairo)"],"NLMid":["101589932"],"ESSN":["2090-908X"]}]}],"pubYear":["2016"],"pageInfo":["8258946"],"abstractText":["The State of Ohio led the United States in measles in 2014, ostensibly related to international air travel (IAT), and ranked lower than 43 other states in infectious disease outbreak preparedness. We conducted a retrospective cohort study using surveillance data of the total Ohio population of 11 million from 2010 through 2014 with a nested case control of air travelers to determine the risk of malaria, seasonal influenza hospitalizations (IH), and hepatitis A (HA) disease related to international travel and to estimate the association with domestic enplanement. IAT appeared protective for HA and IH with a risk of 0.031 (.02-.04) but for malaria was 2.7 (2.07-3.62). Enplanement increased the risk for nonendemic M 3.5 (2.5-4.9) and for HA and IH 1.39 (1.34-1.44). IAT's ratio of relative risk (RRR) of malaria to HA and IH was 87.1 (55.8-136) greater than 219 times versus domestic enplanement which was protective for malaria at 0.397 (0.282-0.559). Malaria is correlated with IAT with cases increasing by 6.9 for every 10,000 passports issued."],"affiliation":["Greene County Public Health, 360 Wilson Drive, Xenia, OH 45385, USA; Wright State University, Dayton, OH 45435, USA; Xavier University, Cincinnati, OH 45207, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4830737?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4830737"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2016/8258946"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-28"],"dateOfCreation":["2016-04-28"],"dateOfRevision":["2016-04-30"],"electronicPublicationDate":["2016-03-31"],"firstPublicationDate":["2016-03-31"],"cprojectID":"PMC4830737"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRShOUS2orL1IQB","_score":1,"_source":{"id":["27144888"],"source":["MED"],"pmid":["27144888"],"pmcid":["PMC4856362"],"doi":["10.1371/journal.pntd.0004694"],"title":["Vector Competence of Aedes aegypti and Aedes polynesiensis Populations from French Polynesia for Chikungunya Virus."],"authorString":["Richard V, Paoaafaite T, Cao-Lormeau VM."],"authorList":[{"author":[{"fullName":["Richard V"],"firstName":["Vaea"],"lastName":["Richard"],"initials":["V"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]},{"fullName":["Paoaafaite T"],"firstName":["Tuterarii"],"lastName":["Paoaafaite"],"initials":["T"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]},{"fullName":["Cao-Lormeau VM"],"firstName":["Van-Mai"],"lastName":["Cao-Lormeau"],"initials":["VM"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004694"],"abstractText":["From October 2014 to March 2015, French Polynesia experienced for the first time a chikungunya outbreak. Two Aedes mosquitoes may have contributed to chikungunya virus (CHIKV) transmission in French Polynesia: the worldwide distributed Ae. aegypti and the Polynesian islands-endemic Ae. polynesiensis mosquito.To investigate the vector competence of French Polynesian populations of Ae. aegypti and Ae. polynesiensis for CHIKV, mosquitoes were exposed per os at viral titers of 7 logs tissue culture infectious dose 50%. At 2, 6, 9, 14 and 21 days post-infection (dpi), saliva was collected from each mosquito and inoculated onto C6/36 mosquito cells to check for the presence of CHIKV infectious particles. Legs and body (thorax and abdomen) of each mosquito were also collected at the different dpi and submitted separately to viral RNA extraction and CHIKV real-time RT-PCR.CHIKV infection rate, dissemination and transmission efficiencies ranged from 7-90%, 18-78% and 5-53% respectively for Ae. aegypti and from 39-41%, 3-17% and 0-14% respectively for Ae. polynesiensis, depending on the dpi. Infectious saliva was found as early as 2 dpi for Ae. aegypti and from 6 dpi for Ae. polynesiensis. Our laboratory results confirm that the French Polynesian population of Ae. aegypti is highly competent for CHIKV and they provide clear evidence for Ae. polynesiensis to act as an efficient CHIKV vector.As supported by our findings, the presence of two CHIKV competent vectors in French Polynesia certainly contributed to enabling this virus to quickly disseminate from the urban/peri-urban areas colonized by Ae. aegypti to the most remote atolls where Ae. polynesiensis is predominating. Ae. polynesiensis was probably involved in the recent chikungunya outbreaks in Samoa and the Cook Islands. Moreover, this vector may contribute to the risk for CHIKV to emerge in other Polynesian islands like Fiji, and more particularly Wallis where there is no Ae. aegypti."],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4856362?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4856362"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004694"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-05"],"dateOfRevision":["2016-05-07"],"electronicPublicationDate":["2016-05-04"],"firstPublicationDate":["2016-05-04"],"cprojectID":"PMC4856362"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRxJ9hGurOzZIZ2","_score":1,"_source":{"id":["27158450"],"source":["MED"],"pmid":["27158450"],"pmcid":["PMC4857756"],"title":["Surveillance, insecticide resistance and control of an invasive Aedes aegypti (Diptera: Culicidae) population in California."],"authorString":["Cornel AJ, Holeman J, Nieman CC, Lee Y, Smith C, Amorino M, Brisco KK, Barrera R, Lanzaro GC, Mulligan Iii FS."],"authorList":[{"author":[{"fullName":["Cornel AJ"],"firstName":["Anthony J"],"lastName":["Cornel"],"initials":["AJ"],"affiliation":["Mosquito Control Research Laboratory, Kearney Agricultural Center, Department of Entomology and Nematology, UC Davis, Parlier, CA, USA."]},{"fullName":["Holeman J"],"firstName":["Jodi"],"lastName":["Holeman"],"initials":["J"],"affiliation":["Consolidated Mosquito Abatement District, Selma, CA, USA."]},{"fullName":["Nieman CC"],"firstName":["Catelyn C"],"lastName":["Nieman"],"initials":["CC"],"affiliation":["Vector Genetics Laboratory, School of Veterinary Medicine, UC Davis, Davis, CA, USA."]},{"fullName":["Lee Y"],"firstName":["Yoosook"],"lastName":["Lee"],"initials":["Y"],"affiliation":["Vector Genetics Laboratory, School of Veterinary Medicine, UC Davis, Davis, CA, USA."]},{"fullName":["Smith C"],"firstName":["Charles"],"lastName":["Smith"],"initials":["C"],"affiliation":["Consolidated Mosquito Abatement District, Selma, CA, USA."]},{"fullName":["Amorino M"],"firstName":["Mark"],"lastName":["Amorino"],"initials":["M"],"affiliation":["Consolidated Mosquito Abatement District, Selma, CA, USA."]},{"fullName":["Brisco KK"],"firstName":["Katherine K"],"lastName":["Brisco"],"initials":["KK"],"affiliation":["Mosquito Control Research Laboratory, Kearney Agricultural Center, Department of Entomology and Nematology, UC Davis, Parlier, CA, USA."]},{"fullName":["Barrera R"],"firstName":["Roberto"],"lastName":["Barrera"],"initials":["R"],"affiliation":["Entomology and Ecology Actvity7, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico."]},{"fullName":["Lanzaro GC"],"firstName":["Gregory C"],"lastName":["Lanzaro"],"initials":["GC"],"affiliation":["Vector Genetics Laboratory, School of Veterinary Medicine, UC Davis, Davis, CA, USA."]},{"fullName":["Mulligan Iii FS"],"firstName":["F Stephen"],"lastName":["Mulligan Iii"],"initials":["FS"],"affiliation":["Consolidated Mosquito Abatement District, Selma, CA, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["194"],"abstractText":["The invasion and subsequent establishment in California of Aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. Studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. Overall, BG Sentinel (BGS) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. Autocidal-Gravid-Ovitraps (AGO-B), when placed at a site for a week, performed equally to BGS in detecting the presence of female Ae. aegypti. Considering operational cost and our findings, we recommend use of BGS traps for surveillance in response to service requests especially in locations outside the known infestation area. We recommend AGO-Bs be placed at fixed sites, cleared and processed once a week to monitor mosquito abundance within a known infestation area. Long-term high density placements of AGO-Bs were found to show promise as an environmentally friendly trap-kill control strategy. California Ae. aegypti were found to be homozygous for the V1016I mutation in the voltage gated sodium channel gene, which is implicated to be involved in insecticide resistance. This strain originating from Clovis, California was resistant to some pyrethroids but not to deltamethrin in bottle bio-assays. Sentinel cage ultra-low-volume (ULV) trials using a new formulation of deltamethrin (DeltaGard®) demonstrated that it provided some control (average of 56% death in sentinel cages in a 91.4 m spray swath) after a single truck mounted aerial ULV application in residential areas."],"affiliation":["Mosquito Control Research Laboratory, Kearney Agricultural Center, Department of Entomology and Nematology, UC Davis, Parlier, CA, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857756?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857756"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," doi"," "]}],"dateOfCompletion":["2016-05-24"],"dateOfCreation":["2016-05-24"],"electronicPublicationDate":["2016-02-19"],"firstPublicationDate":["2016-02-19"],"cprojectID":"PMC4857756"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXoJ9hGurOzZIaC","_score":1,"_source":{"id":["27174284"],"source":["MED"],"pmid":["27174284"],"pmcid":["PMC4866861"],"doi":["10.1128/genomea.00377-16"],"title":["Complete Genome Sequences of Five Zika Virus Isolates."],"authorString":["Ladner JT, Wiley MR, Prieto K, Yasuda CY, Nagle E, Kasper MR, Reyes D, Vasilakis N, Heang V, Weaver SC, Haddow A, Tesh RB, Sovann L, Palacios G."],"authorList":[{"author":[{"fullName":["Ladner JT"],"firstName":["Jason T"],"lastName":["Ladner"],"initials":["JT"],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Wiley MR"],"firstName":["Michael R"],"lastName":["Wiley"],"initials":["MR"],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Prieto K"],"firstName":["Karla"],"lastName":["Prieto"],"initials":["K"],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Yasuda CY"],"firstName":["Chadwick Y"],"lastName":["Yasuda"],"initials":["CY"],"affiliation":["U.S. Naval Medical Research Unit No. 2, Det Phnom Penh (NAMRU-2), Cambodia."]},{"fullName":["Nagle E"],"firstName":["Elyse"],"lastName":["Nagle"],"initials":["E"],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Kasper MR"],"firstName":["Matthew R"],"lastName":["Kasper"],"initials":["MR"],"affiliation":["Naval Medical Research Center, Silver Spring, Maryland, USA."]},{"fullName":["Reyes D"],"firstName":["Daniel"],"lastName":["Reyes"],"initials":["D"],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Vasilakis N"],"firstName":["Nikolaos"],"lastName":["Vasilakis"],"initials":["N"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA."]},{"fullName":["Heang V"],"firstName":["Vireak"],"lastName":["Heang"],"initials":["V"],"affiliation":["U.S. Naval Medical Research Unit No. 2, Det Phnom Penh (NAMRU-2), Cambodia."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA."]},{"fullName":["Haddow A"],"firstName":["Andrew"],"lastName":["Haddow"],"initials":["A"],"affiliation":["Division of Virology, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."]},{"fullName":["Tesh RB"],"firstName":["Robert B"],"lastName":["Tesh"],"initials":["RB"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA."]},{"fullName":["Sovann L"],"firstName":["Ly"],"lastName":["Sovann"],"initials":["L"],"affiliation":["Communicable Disease Centre, Cambodian Ministry of Health, Phnom Penh, Cambodia."]},{"fullName":["Palacios G"],"firstName":["Gustavo"],"lastName":["Palacios"],"initials":["G"],"authorId":[{"_":"0000-0001-5062-1938","$":{"type":"ORCID"}}],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA gustavo.f.palacios.ctr@mail.mil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-5062-1938","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["4"],"journalIssueId":["2405937"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome announcements"],"ISOAbbreviation":["Genome Announc"],"medlineAbbreviation":["Genome Announc"],"NLMid":["101595808"],"ESSN":["2169-8287"]}]}],"pubYear":["2016"],"abstractText":["Zika virus is an emerging human pathogen of great concern due to putative links to microcephaly and Guillain-Barre syndrome. Here, we report the complete genomes, including the 5' and 3' untranslated regions, of five Zika virus isolates, one from the Asian lineage and four from the African lineage."],"affiliation":["Center for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4866861?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4866861"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00377-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-05-13"],"dateOfCreation":["2016-05-13"],"dateOfRevision":["2016-05-19"],"electronicPublicationDate":["2016-05-12"],"firstPublicationDate":["2016-05-12"],"cprojectID":"PMC4866861"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYQ_8VqgcuJwviJ","_score":1,"_source":{"id":["27212842"],"source":["MED"],"pmid":["27212842"],"pmcid":["PMC4857463"],"doi":["10.6026/97320630012028"],"title":["Hypothalamus-Pituitary-Adrenal cell-mediated immunity regulation in the Immune Restoration Inflammatory Syndrome."],"authorString":["Khakshooy A, Chiappelli F."],"authorList":[{"author":[{"fullName":["Khakshooy A"],"firstName":["Allen"],"lastName":["Khakshooy"],"initials":["A"],"affiliation":["Oral Biology and Medicine, Center for the health Sciences University of California Los Angeles, USA; Health Sciences, California State University, Northridge, USA."]},{"fullName":["Chiappelli F"],"firstName":["Francesco"],"lastName":["Chiappelli"],"initials":["F"],"affiliation":["Oral Biology and Medicine, Center for the health Sciences University of California Los Angeles, USA; Evidence-Based Decisions Practice-Based Research Network, USA."]}]}],"journalInfo":[{"issue":["1"],"volume":["12"],"journalIssueId":["2410688"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Bioinformation"],"ISOAbbreviation":["Bioinformation"],"medlineAbbreviation":["Bioinformation"],"NLMid":["101258255"],"ESSN":["0973-2063"]}]}],"pubYear":["2016"],"pageInfo":["28-31"],"abstractText":["Over one third of the patients sero-positive for the human immunodeficiency virus (HIV) with signs of the acquired immune deficiency syndrome (AIDS), and under treatment with anti-retroviral therapy (ART), develop the immune reconstitution inflammatory syndrome (IRIS). It is not clear what variables are that determine whether a patient with HIV/AIDS will develop ART-related IRIS, but the best evidence base thus far indicates that HIV/AIDS patients with low CD4 cell count, and HIV/AIDS patients whose CD4 count recovery shows a sharp slope, suggesting a particularly fast \"immune reconstitution\", are at greater risk of developing IRIS. Here, we propose the hypothesis that one important variable that can contribute to low CD4 cell count number and function in ART-treated HIV/AIDS patients is altered hypothalamic-pituitary-adrenal (HPA) cell-mediated immune (CMI) regulation. We discuss HPA-CMI deregulation in IRIS as the new frontier in comparative effectiveness research (CRE) for obtaining and utilizing the best evidence base for treatment of patients with HIV/AIDS in specific clinical settings. We propose that our hypothesis about altered HPA-CMI may extend to the pathologies observed in related viral infection, including Zika."],"affiliation":["Oral Biology and Medicine, Center for the health Sciences University of California Los Angeles, USA; Health Sciences, California State University, Northridge, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857463?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857463"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.6026/97320630012028"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-23"],"dateOfCreation":["2016-05-23"],"dateOfRevision":["2016-05-25"],"electronicPublicationDate":["2016-01-31"],"firstPublicationDate":["2016-01-31"],"cprojectID":"PMC4857463"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYC_8VqgcuJwviI","_score":1,"_source":{"id":["27144981"],"source":["MED"],"pmid":["27144981"],"pmcid":["PMC4856356"],"doi":["10.1371/journal.pntd.0004696"],"title":["Multi-country Survey Revealed Prevalent and Novel F1534S Mutation in Voltage-Gated Sodium Channel (VGSC) Gene in Aedes albopictus."],"authorString":["Xu J, Bonizzoni M, Zhong D, Zhou G, Cai S, Li Y, Wang X, Lo E, Lee R, Sheen R, Duan J, Yan G, Chen XG."],"authorList":[{"author":[{"fullName":["Xu J"],"firstName":["Jiabao"],"lastName":["Xu"],"initials":["J"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Bonizzoni M"],"firstName":["Mariangela"],"lastName":["Bonizzoni"],"initials":["M"],"affiliation":["Department of Biology and Biotechnology, University of Pavia, Pavia, Italy."]},{"fullName":["Zhong D"],"firstName":["Daibin"],"lastName":["Zhong"],"initials":["D"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Zhou G"],"firstName":["Guofa"],"lastName":["Zhou"],"initials":["G"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Cai S"],"firstName":["Songwu"],"lastName":["Cai"],"initials":["S"],"affiliation":["Department of Vector Control, Centers for Disease Control and Prevention of Guangdong Province, Guangzhou, China."]},{"fullName":["Li Y"],"firstName":["Yiji"],"lastName":["Li"],"initials":["Y"],"affiliation":["Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China."]},{"fullName":["Wang X"],"firstName":["Xiaoming"],"lastName":["Wang"],"initials":["X"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Lo E"],"firstName":["Eugenia"],"lastName":["Lo"],"initials":["E"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Lee R"],"firstName":["Rebecca"],"lastName":["Lee"],"initials":["R"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Sheen R"],"firstName":["Roger"],"lastName":["Sheen"],"initials":["R"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Duan J"],"firstName":["Jinhua"],"lastName":["Duan"],"initials":["J"],"affiliation":["Department of Vector Control, Centers for Disease Control and Prevention of Guangdong Province, Guangzhou, China."]},{"fullName":["Yan G"],"firstName":["Guiyun"],"lastName":["Yan"],"initials":["G"],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."]},{"fullName":["Chen XG"],"firstName":["Xiao-Guang"],"lastName":["Chen"],"initials":["XG"],"affiliation":["Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004696"],"abstractText":["Aedes albopictus is an important dengue vector because of its aggressive biting behavior and rapid spread out of its native home range in Southeast Asia. Pyrethroids are widely used for adult mosquito control, and resistance to pyrethroids should be carefully monitored because vector control is the only effective method currently available to prevent dengue transmission. The voltage-gated sodium channel gene is the target site of pyrethroids, and mutations in this gene cause knockdown resistance (kdr). Previous studies reported various mutations in the voltage-gated sodium channel (VGSC) gene, but the spatial distribution of kdr mutations in Ae. albopictus has not been systematically examined, and the association between kdr mutation and phenotypic resistance has not been established.A total of 597 Ae. albopictus individuals from 12 populations across Asia, Africa, America and Europe were examined for mutations in the voltage-gated sodium channel gene. Three domains for a total of 1,107 bp were sequenced for every individual. Two populations from southern China were examined for pyrethroid resistance using the World Health Organization standard tube bioassay, and the association between kdr mutations and phenotypic resistance was tested.A total of 29 synonymous mutations were found across domain II, III and IV of the VGSC gene. Non-synonymous mutations in two codons of the VGSC gene were detected in 5 populations from 4 countries. A novel mutation at 1532 codon (I1532T) was found in Rome, Italy with a frequency of 19.7%. The second novel mutation at codon 1534 (F1534S) was detected in southern China and Florida, USA with a frequency ranging from 9.5-22.6%. The WHO insecticide susceptibility bioassay found 90.1% and 96.1% mortality in the two populations from southern China, suggesting resistance and probable resistance. Positive association between kdr mutations with deltamethrin resistance was established in these two populations.Two novel kdr mutations, I1532T and F1534S were found in Ae. albopictus. This is the first report of I1532T mutations in Italy and F1534S mutation in China and US. Significant association between kdr mutation and protection from deltamethrin raised the possibility that kdr mutation may be a viable biomarker for pyrethroid resistance surveillance in Ae. albopictus. The patchy distribution of kdr mutations in Ae. albopictus mosquitoes calls for developing global surveillance plan for pyrethroid resistance and developing countermeasures to mitigate the spread of resistance."],"affiliation":["Program in Public Health, University of California, Irvine, Irvine, California, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4856356?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4856356"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004696"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-05"],"dateOfRevision":["2016-05-07"],"electronicPublicationDate":["2016-05-04"],"firstPublicationDate":["2016-05-04"],"cprojectID":"PMC4856356"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrZA_8VqgcuJwviN","_score":1,"_source":{"id":["27159058"],"source":["MED"],"pmid":["27159058"],"pmcid":["PMC4861341"],"doi":["10.1371/journal.pntd.0004704"],"title":["Using Rapid Diagnostic Tests as a Source of Viral RNA for Dengue Serotyping by RT-PCR - A Novel Epidemiological Tool."],"authorString":["Vongsouvath M, Phommasone K, Sengvilaipaseuth O, Kosoltanapiwat N, Chantratita N, Blacksell SD, Lee SJ, de Lamballerie X, Mayxay M, Keomany S, Newton PN, Dubot-Pérès A."],"authorList":[{"author":[{"fullName":["Vongsouvath M"],"firstName":["Manivanh"],"lastName":["Vongsouvath"],"initials":["M"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Phommasone K"],"firstName":["Koukeo"],"lastName":["Phommasone"],"initials":["K"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Sengvilaipaseuth O"],"firstName":["Onanong"],"lastName":["Sengvilaipaseuth"],"initials":["O"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Kosoltanapiwat N"],"firstName":["Nathamon"],"lastName":["Kosoltanapiwat"],"initials":["N"],"affiliation":["Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Salaya, Thailand."]},{"fullName":["Chantratita N"],"firstName":["Narisara"],"lastName":["Chantratita"],"initials":["N"],"affiliation":["Mahidol - Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Salaya, Thailand."]},{"fullName":["Blacksell SD"],"firstName":["Stuart D"],"lastName":["Blacksell"],"initials":["SD"],"authorId":[{"_":"0000-0001-6576-726X","$":{"type":"ORCID"}}],"affiliation":["Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom."]},{"fullName":["Lee SJ"],"firstName":["Sue J"],"lastName":["Lee"],"initials":["SJ"],"affiliation":["Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom."]},{"fullName":["de Lamballerie X"],"firstName":["Xavier"],"lastName":["de Lamballerie"],"initials":["X"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University - IRD 190 - Inserm 1207 - EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Mayxay M"],"firstName":["Mayfong"],"lastName":["Mayxay"],"initials":["M"],"affiliation":["Faculty of Postgraduate Studies, University of Health Sciences, Vientiane, Lao PDR."]},{"fullName":["Keomany S"],"firstName":["Sommay"],"lastName":["Keomany"],"initials":["S"],"affiliation":["Salavan Provincial Hospital, Salavan Provincial Health Department, Salavan Province, Laos."]},{"fullName":["Newton PN"],"firstName":["Paul N"],"lastName":["Newton"],"initials":["PN"],"affiliation":["Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom."]},{"fullName":["Dubot-Pérès A"],"firstName":["Audrey"],"lastName":["Dubot-Pérès"],"initials":["A"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University - IRD 190 - Inserm 1207 - EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-6576-726X","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004704"],"abstractText":["Dengue virus infection causes major public health problems in tropical and subtropical areas. In many endemic areas, including the Lao PDR, inadequate access to laboratory facilities is a major obstacle to surveillance and study of dengue epidemiology. Filter paper is widely used for blood collection for subsequent laboratory testing for antibody and nucleic acid detection. For the first time, we demonstrate that dengue viral RNA can be extracted from dengue rapid diagnostic tests (RDT) and then submitted to real-time RT-PCR for serotyping.We evaluated the Standard Diagnostics (SD) Bioline Dengue Duo RDT, a commonly used test in dengue endemic areas. First, using the QIAamp RNA kit, dengue RNA was purified from the sample pad of the NS1 RDT loaded with virus isolates of the four serotypes, then quantified by RT-PCR. We observed greater recovery of virus, with a mean of 27 times more RNA recovered from RDT, than from filter paper. Second, we evaluated dengue NS1 RDTs from patients at Mahosot Hospital, Vientiane, (99 patients) and from rural Salavan Provincial Hospital (362 patients). There was good agreement between dengue RT-PCR from NS1 RDT with RT-PCR performed on RNA extracted from patient sera, either using RDT loaded with blood (82.8% and 91.4%, in Vientiane and Salavan, respectively) or serum (91.9% and 93.9%). There was 100% concordance between RDT and serum RT-PCR of infecting dengue serotype.Therefore, the collection of NS1 positive RDTs, which do not require cold storage, may be a novel approach for dengue serotyping by RT-PCR and offers promising prospects for the collection of epidemiological data from previously inaccessible tropical areas to aid surveillance and public health interventions."],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861341?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861341"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004704"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-10"],"dateOfRevision":["2016-05-14"],"electronicPublicationDate":["2016-05-09"],"firstPublicationDate":["2016-05-09"],"cprojectID":"PMC4861341"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrZFJ9hGurOzZIaJ","_score":1,"_source":{"id":["27143489"],"source":["MED"],"pmid":["27143489"],"pmcid":["PMC4878300"],"doi":["10.1590/0074-02760150409"],"title":["Spatial and temporal country-wide survey of temephos resistance in Brazilian populations of Aedes aegypti."],"authorString":["Chediak M, G Pimenta F, Coelho GE, Braga IA, Lima JB, Cavalcante KR, Sousa LC, Melo-Santos MA, Macoris Mde L, Araújo AP, Ayres CF, Andrighetti MT, Gomes RG, Campos KB, Guedes RN."],"authorList":[{"author":[{"fullName":["Chediak M"],"firstName":["Mateus"],"lastName":["Chediak"],"initials":["M"],"affiliation":["Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brasil."]},{"fullName":["G Pimenta F Jr"],"firstName":["Fabiano"],"lastName":["G Pimenta"],"initials":["F"],"affiliation":["Secretaria Municipal de Saúde de Belo Horizonte, Belo Horizonte, MG, Brasil."]},{"fullName":["Coelho GE"],"firstName":["Giovanini E"],"lastName":["Coelho"],"initials":["GE"],"affiliation":["Secretaria de Vigilância em Saúde, Ministério da Saúde, Brasília, DF, Brasil."]},{"fullName":["Braga IA"],"firstName":["Ima A"],"lastName":["Braga"],"initials":["IA"],"affiliation":["Secretaria de Vigilância em Saúde, Ministério da Saúde, Brasília, DF, Brasil."]},{"fullName":["Lima JB"],"firstName":["José Bento P"],"lastName":["Lima"],"initials":["JB"],"affiliation":["Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Cavalcante KR"],"firstName":["Karina Ribeiro Lj"],"lastName":["Cavalcante"],"initials":["KR"],"affiliation":["Secretaria de Vigilância em Saúde, Ministério da Saúde, Brasília, DF, Brasil."]},{"fullName":["Sousa LC"],"firstName":["Lindemberg C de"],"lastName":["Sousa"],"initials":["LC"],"affiliation":["Laboratório de Entomologia, Secretaria de Saúde do Ceará, Fortaleza, CE, Brasil."]},{"fullName":["Melo-Santos MA"],"firstName":["Maria Alice V de"],"lastName":["Melo-Santos"],"initials":["MA"],"affiliation":["Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil."]},{"fullName":["Macoris Mde L"],"firstName":["Maria de Lourdes da G"],"lastName":["Macoris"],"initials":["Mde L"],"affiliation":["Secretaria de Saúde de São Paulo, Marília, SP, Brasil."]},{"fullName":["Araújo AP"],"firstName":["Ana Paula de"],"lastName":["Araújo"],"initials":["AP"],"affiliation":["Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil."]},{"fullName":["Ayres CF"],"firstName":["Constância Flávia J"],"lastName":["Ayres"],"initials":["CF"],"affiliation":["Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil."]},{"fullName":["Andrighetti MT"],"firstName":["Maria Teresa M"],"lastName":["Andrighetti"],"initials":["MT"],"affiliation":["Secretaria de Saúde de São Paulo, Marília, SP, Brasil."]},{"fullName":["Gomes RG"],"firstName":["Ricristhi Gonçalves de A"],"lastName":["Gomes"],"initials":["RG"],"affiliation":["Laboratório de Entomologia, Secretaria de Saúde do Ceará, Fortaleza, CE, Brasil."]},{"fullName":["Campos KB"],"firstName":["Kauara B"],"lastName":["Campos"],"initials":["KB"],"affiliation":["Secretaria de Vigilância em Saúde, Ministério da Saúde, Brasília, DF, Brasil."]},{"fullName":["Guedes RN"],"firstName":["Raul Narciso C"],"lastName":["Guedes"],"initials":["RN"],"affiliation":["Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brasil."]}]}],"journalInfo":[{"issue":["5"],"volume":["111"],"journalIssueId":["2412036"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["311-321"],"abstractText":["The organophosphate temephos has been the main insecticide used against larvae of the dengue and yellow fever mosquito (Aedes aegypti) in Brazil since the mid-1980s. Reports of resistance date back to 1995; however, no systematic reports of widespread temephos resistance have occurred to date. As resistance investigation is paramount for strategic decision-making by health officials, our objective here was to investigate the spatial and temporal spread of temephos resistance in Ae. aegypti in Brazil for the last 12 years using discriminating temephos concentrations and the bioassay protocols of the World Health Organization. The mortality results obtained were subjected to spatial analysis for distance interpolation using semi-variance models to generate maps that depict the spread of temephos resistance in Brazil since 1999. The problem has been expanding. Since 2002-2003, approximately half the country has exhibited mosquito populations resistant to temephos. The frequency of temephos resistance and, likely, control failures, which start when the insecticide mortality level drops below 80%, has increased even further since 2004. Few parts of Brazil are able to achieve the target 80% efficacy threshold by 2010/2011, resulting in a significant risk of control failure by temephos in most of the country. The widespread resistance to temephos in Brazilian Ae. aegypti populations greatly compromise effective mosquito control efforts using this insecticide and indicates the urgent need to identify alternative insecticides aided by the preventive elimination of potential mosquito breeding sites."],"affiliation":["Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878300?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878300"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760150409"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-26"],"dateOfRevision":["2016-05-26"],"electronicPublicationDate":["2016-04-29"],"firstPublicationDate":["2016-04-29"],"cprojectID":"PMC4878300"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraShOUS2orL1IQd","_score":1,"_source":{"id":["27274849"],"source":["MED"],"pmid":["27274849"],"pmcid":["PMC4879239"],"doi":["10.1016/j.nmni.2016.02.013"],"title":["Complete coding sequence of Zika virus from Martinique outbreak in 2015."],"authorString":["Piorkowski G, Richard P, Baronti C, Gallian P, Charrel R, Leparc-Goffart I, de Lamballerie X."],"authorList":[{"author":[{"fullName":["Piorkowski G"],"firstName":["G"],"lastName":["Piorkowski"],"initials":["G"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Richard P"],"firstName":["P"],"lastName":["Richard"],"initials":["P"],"affiliation":["Etablissement Français du Sang-Fort de France, Martinique, France."]},{"fullName":["Baronti C"],"firstName":["C"],"lastName":["Baronti"],"initials":["C"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Gallian P"],"firstName":["P"],"lastName":["Gallian"],"initials":["P"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France; Etablissement Français du Sang Alpes Méditerranée, Marseille, France."]},{"fullName":["Charrel R"],"firstName":["R"],"lastName":["Charrel"],"initials":["R"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Leparc-Goffart I"],"firstName":["I"],"lastName":["Leparc-Goffart"],"initials":["I"],"affiliation":["French National Reference Centre for Arboviruses, IRBA Armed Forces Biomedical Research Institute, Marseille, France."]},{"fullName":["de Lamballerie X"],"firstName":["X"],"lastName":["de Lamballerie"],"initials":["X"],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]}]}],"journalInfo":[{"volume":["11"],"journalIssueId":["2384654"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["New microbes and new infections"],"ISOAbbreviation":["New Microbes New Infect"],"medlineAbbreviation":["New Microbes New Infect"],"NLMid":["101624750"],"ESSN":["2052-2975"]}]}],"pubYear":["2016"],"pageInfo":["52-53"],"abstractText":["Zika virus is an Aedes-borne Flavivirus causing fever, arthralgia, myalgia rash, associated with Guillain-Barré syndrome and suspected to induce microcephaly in the fetus. We report here the complete coding sequence of the first characterized Caribbean Zika virus strain, isolated from a patient from Martinique in December, 2015."],"affiliation":["UMR \"Emergence des Pathologies Virales\" (EPV: Aix-Marseille University-IRD 190-Inserm 1207-EHESP) & Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","outbreak","Americas","Microcephaly","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4879239?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4879239"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.nmni.2016.02.013"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-06-09"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-03-08"],"firstPublicationDate":["2016-03-08"],"cprojectID":"PMC4879239"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrcFJ9hGurOzZIaZ","_score":1,"_source":{"id":["27139439"],"source":["MED"],"pmid":["27139439"],"pmcid":["PMC4889731"],"doi":["10.4269/ajtmh.16-0277"],"title":["The Development of Small Animal Models for Zika Virus Vaccine Efficacy Testing and Pathological Assessment."],"authorString":["Brault AC, Bowen RA."],"authorList":[{"author":[{"fullName":["Brault AC"],"firstName":["Aaron C"],"lastName":["Brault"],"initials":["AC"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado abrault@cdc.gov."]},{"fullName":["Bowen RA"],"firstName":["Richard A"],"lastName":["Bowen"],"initials":["RA"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado."]}]}],"journalInfo":[{"issue":["6"],"volume":["94"],"journalIssueId":["2414127"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2016"],"pageInfo":["1187-1188"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado abrault@cdc.gov."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889731?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889731"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.16-0277"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-02"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-05-02"],"firstPublicationDate":["2016-05-02"],"cprojectID":"PMC4889731"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnreLJ9hGurOzZIad","_score":1,"_source":{"id":["27258065"],"source":["MED"],"pmid":["27258065"],"pmcid":["PMC4892518"],"doi":["10.1371/journal.pntd.0004760"],"title":["First International Workshop on Zika Virus Held by Oswaldo Cruz Foundation FIOCRUZ in Northeast Brazil March 2016 - A Meeting Report."],"authorString":["Franca RF, Neves MH, Ayres CF, Melo-Neto OP, Filho SP."],"authorList":[{"author":[{"fullName":["Franca RF"],"firstName":["Rafael F O"],"lastName":["Franca"],"initials":["RF"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."]},{"fullName":["Neves MH"],"firstName":["Maria Helena L"],"lastName":["Neves"],"initials":["MH"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."]},{"fullName":["Ayres CF"],"firstName":["Constancia F Junqueira"],"lastName":["Ayres"],"initials":["CF"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."]},{"fullName":["Melo-Neto OP"],"firstName":["Osvaldo P"],"lastName":["Melo-Neto"],"initials":["OP"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."]},{"fullName":["Filho SP"],"firstName":["Sinval P Brandão"],"lastName":["Filho"],"initials":["SP"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."]}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004760"],"affiliation":["Instituto Aggeu Magalhães (IAM), Fundação Oswaldo Cruz (Fiocruz), Recife, Pernambuco, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4892518?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4892518"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004760"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-04"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-06-03"],"firstPublicationDate":["2016-06-03"],"cprojectID":"PMC4892518"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfmhOUS2orL1IQx","_score":1,"_source":{"id":["27376056"],"source":["MED"],"pmid":["27376056"],"pmcid":["PMC4899437"],"doi":["10.3389/fpubh.2016.00120"],"title":["The Ebola Virus Disease Outbreak in West Africa: A Wake-up Call to Revitalize Implementation of the International Health Regulations."],"authorString":["Olu OO."],"authorList":[{"author":[{"fullName":["Olu OO"],"firstName":["Olushayo Oluseun"],"lastName":["Olu"],"initials":["OO"],"affiliation":["World Health Organization , Kigali , Rwanda."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-6832-873X","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2374588"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in public health"],"ISOAbbreviation":["Front Public Health"],"medlineAbbreviation":["Front Public Health"],"NLMid":["101616579"],"ESSN":["2296-2565"]}]}],"pubYear":["2016"],"pageInfo":["120"],"abstractText":["The 2014/15 Ebola virus disease (EVD) outbreak in West Africa has highlighted the inherent weaknesses associated with the implementation of the International Health Regulations (IHR). In this perspective article, the lessons learnt from the outbreak are used to review the challenges impeding effective implementation of the IHR and to propose policy and strategic options for enhancing its application. While some progress has been achieved in implementing the IHR in several countries, numerous challenges continue to impede its effectiveness, especially in developing countries, such as those affected by the West Africa EVD outbreak. Political and economic sensitivities associated with reporting public health emergencies of international concern (PHEIC), inadequate resources (human and financial), and lack of technical know-how required for implementation of the IHR are weaknesses that continue to constrain the implementation of the regulations. In view of the complex sociopolitical, cultural, and public health dimensions of PHEICs, frameworks, such as the IHR, which have legal backing, seem to be the most effective and sustainable option for assuring timely detection, notification, and response to such events. Renewed efforts to strengthen national and global institutional frameworks for implementation of the IHR are therefore required. Improvements in transparency, commitment, and accountability of parties to the IHR, mainstreaming of the IHR into national public health governance structures, use of multidisciplinary approaches, and mobilization of the required resources for the implementation of the IHR are imperative."],"affiliation":["World Health Organization , Kigali , Rwanda."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["outbreak","International Health Regulations","West Africa","Ebola Virus Disease","Improved Implementation"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4899437?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4899437"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fpubh.2016.00120"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-04"],"dateOfCreation":["2016-07-04"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-09"],"firstPublicationDate":["2016-06-09"],"cprojectID":"PMC4899437"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrgDhOUS2orL1IQ0","_score":1,"_source":{"id":["27293547"],"source":["MED"],"pmid":["27293547"],"pmcid":["PMC4902960"],"doi":["10.1186/s13578-016-0110-x"],"title":["Bioinformatic analysis reveals the expression of unique transcriptomic signatures in Zika virus infected human neural stem cells."],"authorString":["Rolfe AJ, Bosco DB, Wang J, Nowakowski RS, Fan J, Ren Y."],"authorList":[{"author":[{"fullName":["Rolfe AJ"],"firstName":["Alyssa J"],"lastName":["Rolfe"],"initials":["AJ"],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."]},{"fullName":["Bosco DB"],"firstName":["Dale B"],"lastName":["Bosco"],"initials":["DB"],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."]},{"fullName":["Wang J"],"firstName":["Jingying"],"lastName":["Wang"],"initials":["J"],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."]},{"fullName":["Nowakowski RS"],"firstName":["Richard S"],"lastName":["Nowakowski"],"initials":["RS"],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."]},{"fullName":["Fan J"],"firstName":["Jianqing"],"lastName":["Fan"],"initials":["J"],"affiliation":["Statistical Laboratory, Princeton University, Princeton, NJ 08540 USA."]},{"fullName":["Ren Y"],"firstName":["Yi"],"lastName":["Ren"],"initials":["Y"],"authorId":[{"_":"0000-0002-0176-493X","$":{"type":"ORCID"}}],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0176-493X","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2367765"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Cell & bioscience"],"ISOAbbreviation":["Cell Biosci"],"medlineAbbreviation":["Cell Biosci"],"NLMid":["101561195"],"ESSN":["2045-3701"]}]}],"pubYear":["2016"],"pageInfo":["42"],"abstractText":["The single-stranded RNA Flavivirus, Zika virus (ZIKV), has recently re-emerged and spread rapidly across the western hemisphere's equatorial countries, primarily through Aedes mosquito transmission. While symptoms in adult infections appear to be self-limiting and mild, severe birth defects, such as microcephaly, have been linked to infection during early pregnancy. Recently, Tang et al. (Cell Stem Cell 2016, doi: 10.1016/j.stem.2016.02.016) demonstrated that ZIKV efficiently infects induced pluripotent stem cell (iPSC) derived human neural progenitor cells (hNPCs), resulting in cell cycle abnormalities and apoptosis. Consequently, hNPCs are a suggested ZIKV target.We analyzed the transcriptomic sequencing (RNA-seq) data (GEO: GSE78711) of ZIKV (Strain: MR766) infected hNPCs. For comparison to the ZIKV-infected hNPCs, the expression data from hNPCs infected with human cytomegalovirus (CMV) (Strain: AD169) was used (GEO: GSE35295). Utilizing a combination of Gene Ontology, database of human diseases, and pathway analysis, we generated a putative systemic model of infection supported by known molecular pathways of other highly related viruses.We analyzed RNA-sequencing data for transcript expression alterations in ZIKV-infected hNPCs, and then compared them to expression patterns of iPSC-derived hNPCs infected with CMV, a virus that can also induce severe congenital neurological defects in developing fetuses. We demonstrate for the first time that many of cellular pathways correlate with clinical pathologies following ZIKV infection such as microcephaly, congenital nervous system disorders and epilepsy. Furthermore, ZIKV activates several inflammatory signals within infected hNPCs that are implicated in innate and acquired immune responses, while CMV-infected hNPCs showed limited representation of these pathways. Moreover, several genes related to pathogen responses are significantly upregulated upon ZIKV infection, but not perturbed in CMV-infected hNPCs.The presented study is the first to report enrichment of numerous pro-inflammatory pathways in ZIKV-infected hNPCs, indicating that hNPCs are capable of signaling through canonical pro-inflammatory pathways following viral infection. By defining gene expression profiles, new factors in the pathogenesis of ZIKV were identified which could help develop new therapeutic strategies."],"affiliation":["Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Street, Tallahassee, FL 32306 USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["R01 GM100474"],"agency":["NIGMS NIH HHS"],"acronym":["GM"],"orderIn":["0"]},{"grantId":["R01 GM072611"],"agency":["NIGMS NIH HHS"],"acronym":["GM"],"orderIn":["0"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4902960?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4902960"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13578-016-0110-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-13"],"dateOfCreation":["2016-06-13"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-06-10"],"firstPublicationDate":["2016-06-10"],"cprojectID":"PMC4902960"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrgGhOUS2orL1IQ3","_score":1,"_source":{"id":["27304211"],"source":["MED"],"pmid":["27304211"],"pmcid":["PMC4909274"],"doi":["10.1371/journal.pntd.0004762"],"title":["Potential Risk of Dengue and Chikungunya Outbreaks in Northern Italy Based on a Population Model of Aedes albopictus (Diptera: Culicidae)."],"authorString":["Guzzetta G, Montarsi F, Baldacchino FA, Metz M, Capelli G, Rizzoli A, Pugliese A, Rosà R, Poletti P, Merler S."],"authorList":[{"author":[{"fullName":["Guzzetta G"],"firstName":["Giorgio"],"lastName":["Guzzetta"],"initials":["G"],"authorId":[{"_":"0000-0002-9296-9470","$":{"type":"ORCID"}}],"affiliation":["Fondazione Bruno Kessler, Trento, Italy."]},{"fullName":["Montarsi F"],"firstName":["Fabrizio"],"lastName":["Montarsi"],"initials":["F"],"affiliation":["Laboratory of Parasitology, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy."]},{"fullName":["Baldacchino FA"],"firstName":["Frédéric Alexandre"],"lastName":["Baldacchino"],"initials":["FA"],"affiliation":["Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy."]},{"fullName":["Metz M"],"firstName":["Markus"],"lastName":["Metz"],"initials":["M"],"affiliation":["Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy."]},{"fullName":["Capelli G"],"firstName":["Gioia"],"lastName":["Capelli"],"initials":["G"],"affiliation":["Laboratory of Parasitology, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy."]},{"fullName":["Rizzoli A"],"firstName":["Annapaola"],"lastName":["Rizzoli"],"initials":["A"],"affiliation":["Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy."]},{"fullName":["Pugliese A"],"firstName":["Andrea"],"lastName":["Pugliese"],"initials":["A"],"affiliation":["Department of Mathematics, University of Trento, Trento, Italy."]},{"fullName":["Rosà R"],"firstName":["Roberto"],"lastName":["Rosà"],"initials":["R"],"authorId":[{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}],"affiliation":["Department of Biodiversity and Molecular Ecology, Fondazione Edmund Mach, San Michele all'Adige (TN), Italy."]},{"fullName":["Poletti P"],"firstName":["Piero"],"lastName":["Poletti"],"initials":["P"],"affiliation":["Dondena Centre for Research on Social Dynamics and Public Policy, Bocconi University, Milan, Italy."]},{"fullName":["Merler S"],"firstName":["Stefano"],"lastName":["Merler"],"initials":["S"],"affiliation":["Fondazione Bruno Kessler, Trento, Italy."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9296-9470","$":{"type":"ORCID"}},{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004762"],"abstractText":["The rapid invasion and spread of Aedes albopictus (Skuse, 1894) within new continents and climatic ranges has created favorable conditions for the emergence of tropical arboviral diseases in the invaded areas. We used mosquito abundance data from 2014 collected across ten sites in northern Italy to calibrate a population model for Aedes albopictus and estimate the potential of imported human cases of chikungunya or dengue to generate the condition for their autochthonous transmission in the absence of control interventions. The model captured intra-year seasonality and heterogeneity across sites in mosquito abundance, based on local temperature patterns and the estimated site-specific mosquito habitat suitability. A robust negative correlation was found between the latter and local late spring precipitations, indicating a possible washout effect on larval breeding sites. The model predicts a significant risk of chikungunya outbreaks in most sites if a case is imported between the beginning of summer and up to mid-November, with an average outbreak probability between 4.9% and 25%, depending on the site. A lower risk is predicted for dengue, with an average probability between 4.2% and 10.8% for cases imported between mid-July and mid-September. This study shows the importance of an integrated entomological and medical surveillance for the evaluation of arboviral disease risk, which is a precondition for designing cost-effective vector control programs."],"affiliation":["Fondazione Bruno Kessler, Trento, Italy."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909274?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909274"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004762"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-16"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-15"],"firstPublicationDate":["2016-06-15"],"cprojectID":"PMC4909274"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrgSJ9hGurOzZIak","_score":1,"_source":{"id":["27280803"],"source":["MED"],"pmid":["27280803"],"pmcid":["PMC4900517"],"doi":["10.1371/journal.pntd.0004653"],"title":["Dengue Vaccine: Considerations before Rollout in Colombia."],"authorString":["Villabona-Arenas CJ, Ocazionez Jimenez RE, Jimenez Silva CL."],"authorList":[{"author":[{"fullName":["Villabona-Arenas CJ"],"firstName":["Christian Julian"],"lastName":["Villabona-Arenas"],"initials":["CJ"],"affiliation":["Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier (LIRMM), Institut de Biologie Computationnelle (IBC), Université de Montpellier, Montpellier, France."]},{"fullName":["Ocazionez Jimenez RE"],"firstName":["Raquel Elvira"],"lastName":["Ocazionez Jimenez"],"initials":["RE"],"affiliation":["Laboratorio de Arbovirus, Centro de Investigaciones en Enfermedades Tropicales (CINTROP), Universidad Industrial de Santander, Santander, Colombia."]},{"fullName":["Jimenez Silva CL"],"firstName":["Cinthy Lorena"],"lastName":["Jimenez Silva"],"initials":["CL"],"affiliation":["Laboratorio de Arbovirus, Centro de Investigaciones en Enfermedades Tropicales (CINTROP), Universidad Industrial de Santander, Santander, Colombia."]}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004653"],"affiliation":["Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier (LIRMM), Institut de Biologie Computationnelle (IBC), Université de Montpellier, Montpellier, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4900517?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4900517"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004653"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-10"],"dateOfRevision":["2016-06-24"],"electronicPublicationDate":["2016-06-09"],"firstPublicationDate":["2016-06-09"],"cprojectID":"PMC4900517"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhdhOUS2orL1IQ6","_score":1,"_source":{"id":["27366585"],"source":["MED"],"pmid":["27366585"],"pmcid":["PMC4915778"],"doi":["10.1371/currents.outbreaks.1c39ec05a743d41ee39391ed0f2ed8d3"],"title":["Genetically Modified (GM) Mosquito Use to Reduce Mosquito-Transmitted Disease in the US: A Community Opinion Survey."],"authorString":["Adalja A, Sell TK, McGinty M, Boddie C."],"authorList":[{"author":[{"fullName":["Adalja A"],"firstName":["Amesh"],"lastName":["Adalja"],"initials":["A"],"affiliation":["UPMC Center for Health Security, Baltimore, Maryland, USA."]},{"fullName":["Sell TK"],"firstName":["Tara Kirk"],"lastName":["Sell"],"initials":["TK"],"affiliation":["UPMC Center for Health Security, Baltimore, Maryland, USA."]},{"fullName":["McGinty M"],"firstName":["Meghan"],"lastName":["McGinty"],"initials":["M"],"affiliation":["UPMC Center for Health Security, Baltimore, Maryland, USA."]},{"fullName":["Boddie C"],"firstName":["Crystal"],"lastName":["Boddie"],"initials":["C"],"affiliation":["UPMC Center for Health Security, Baltimore, Maryland, USA."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["Mosquito-borne infectious diseases such as dengue, chikungunya, and now Zika, pose a public health threat to the US, particularly Florida, the Gulf Coast states, and Hawaii. Recent autochthonous transmission of dengue and chikungunya in Florida, the recent dengue outbreak in Hawaii, and the potential for future local spread of Zika in the US, has led to the consideration of novel approaches to mosquito management. One such novel approach, the release of sterile genetically modified mosquitoes, has been proposed as a possible intervention, and a trial release of GM mosquitoes is being considered in one Florida community. However, this proposal has been controversial. The objective of this research was to increase understanding of community knowledge, attitudes, and beliefs regarding mosquito control and GM mosquitoes. An 18-question self-administered survey was mailed to all households in the identified Key West, Florida neighborhood where a GM mosquito trial has been proposed. This survey was fielded between July 20, 2015 and November 1, 2015. The main outcome variable was opposition to the use of GM mosquitoes. Measures included demographic information and opinions on mosquitoes, mosquito control, and vector-borne diseases. A majority of survey respondents did not support use of GM mosquitoes as a mosquito control method.Reasons for opposition included general fears about possible harmful impacts of this intervention, specific worries about human and animal health impacts from the GM mosquitoes, and environmental concerns about potential negative effects on the ecosystem. Residents were more likely to oppose GM mosquito use if they had a low perception of the potential risks posed by diseases like dengue and chikungunya, if they were female, and if they were less concerned about the need to control mosquitoes in general. These findings suggest a need for new approaches to risk communication, including educational efforts surrounding mosquito control and reciprocal dialogue between residents and public health officials."],"affiliation":["UPMC Center for Health Security, Baltimore, Maryland, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","GMO","Mosquitoes","Dengue","Chikungunya","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4915778"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.1c39ec05a743d41ee39391ed0f2ed8d3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-01"],"dateOfCreation":["2016-07-01"],"electronicPublicationDate":["2016-05-25"],"firstPublicationDate":["2016-05-25"],"cprojectID":"PMC4915778"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhvJ9hGurOzZIat","_score":1,"_source":{"id":["27312324"],"source":["MED"],"pmid":["27312324"],"pmcid":["PMC4911567"],"doi":["10.1038/srep28070"],"title":["Prevention and Control of Zika as a Mosquito-Borne and Sexually Transmitted Disease: A Mathematical Modeling Analysis."],"authorString":["Gao D, Lou Y, He D, Porco TC, Kuang Y, Chowell G, Ruan S."],"authorList":[{"author":[{"fullName":["Gao D"],"firstName":["Daozhou"],"lastName":["Gao"],"initials":["D"],"affiliation":["Mathematics and Science College, Shanghai Normal University, Shanghai 200234, China."]},{"fullName":["Lou Y"],"firstName":["Yijun"],"lastName":["Lou"],"initials":["Y"],"affiliation":["Department of Applied Mathematics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China."]},{"fullName":["He D"],"firstName":["Daihai"],"lastName":["He"],"initials":["D"],"affiliation":["Department of Applied Mathematics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China."]},{"fullName":["Porco TC"],"firstName":["Travis C"],"lastName":["Porco"],"initials":["TC"],"affiliation":["Francis I Proctor Foundation, Department of Epidemiology and Biostatistics, and Department of Ophthalmology; University of California at San Francisco, San Francisco, CA 94143 USA."]},{"fullName":["Kuang Y"],"firstName":["Yang"],"lastName":["Kuang"],"initials":["Y"],"affiliation":["School of Mathematical and Statistical Sciences, Arizona State University, Tempe, AZ 85287, USA."]},{"fullName":["Chowell G"],"firstName":["Gerardo"],"lastName":["Chowell"],"initials":["G"],"affiliation":["School of Public Health, Georgia State University, Atlanta, GA 30302, USA."]},{"fullName":["Ruan S"],"firstName":["Shigui"],"lastName":["Ruan"],"initials":["S"],"affiliation":["Department of Mathematics, University of Miami, Coral Gables, FL 33146, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-3253-654X","$":{"type":"ORCID"}},{"_":"0000-0003-3864-2001","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["28070"],"abstractText":["The ongoing Zika virus (ZIKV) epidemic in the Americas poses a major global public health emergency. While ZIKV is transmitted from human to human by bites of Aedes mosquitoes, recent evidence indicates that ZIKV can also be transmitted via sexual contact with cases of sexually transmitted ZIKV reported in Argentina, Canada, Chile, France, Italy, New Zealand, Peru, Portugal, and the USA. Yet, the role of sexual transmission on the spread and control of ZIKV infection is not well-understood. We introduce a mathematical model to investigate the impact of mosquito-borne and sexual transmission on the spread and control of ZIKV and calibrate the model to ZIKV epidemic data from Brazil, Colombia, and El Salvador. Parameter estimates yielded a basic reproduction number 0 = 2.055 (95% CI: 0.523-6.300), in which the percentage contribution of sexual transmission is 3.044% (95% CI: 0.123-45.73). Our sensitivity analyses indicate that 0 is most sensitive to the biting rate and mortality rate of mosquitoes while sexual transmission increases the risk of infection and epidemic size and prolongs the outbreak. Prevention and control efforts against ZIKV should target both the mosquito-borne and sexual transmission routes."],"affiliation":["Mathematics and Science College, Shanghai Normal University, Shanghai 200234, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4911567?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4911567"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep28070"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-06-21"],"electronicPublicationDate":["2016-06-17"],"firstPublicationDate":["2016-06-17"],"cprojectID":"PMC4911567"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnriw_8VqgcuJwvir","_score":1,"_source":{"id":["27249063"],"source":["MED"],"pmid":["27249063"],"pmcid":["PMC4889139"],"doi":["10.1371/journal.pntd.0004769"],"title":["Control of Urban Zika Vectors: Should We Return to the Successful PAHO/WHO Strategy?"],"authorString":["Reiter P."],"authorList":[{"author":[{"fullName":["Reiter P"],"firstName":["Paul"],"lastName":["Reiter"],"initials":["P"],"affiliation":["Institut Pasteur, Paris, France."]}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004769"],"affiliation":["Institut Pasteur, Paris, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889139?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889139"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004769"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-02"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-06-01"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4889139"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrir_8VqgcuJwviq","_score":1,"_source":{"id":["27229686"],"source":["MED"],"pmid":["27229686"],"pmcid":["PMC4882898"],"doi":["10.1186/s13071-016-1577-3"],"title":["Spread and establishment of Aedes albopictus in southern Switzerland between 2003 and 2014: an analysis of oviposition data and weather conditions."],"authorString":["Flacio E, Engeler L, Tonolla M, Müller P."],"authorList":[{"author":[{"fullName":["Flacio E"],"firstName":["Eleonora"],"lastName":["Flacio"],"initials":["E"],"affiliation":["Laboratory of Eco-Epidemiology of Parasites, Institute of Biology, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland. eleonora.flacio@supsi.ch."]},{"fullName":["Engeler L"],"firstName":["Lukas"],"lastName":["Engeler"],"initials":["L"],"affiliation":["Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland, via Mirasole 22A, 6500, Bellinzona, Switzerland."]},{"fullName":["Tonolla M"],"firstName":["Mauro"],"lastName":["Tonolla"],"initials":["M"],"affiliation":["Microbiology Unit, Plant Biology Department, Sciences III University of Geneva, Quai Ernest-Ansermet 30, 1211, Geneva, Switzerland."]},{"fullName":["Müller P"],"firstName":["Pie"],"lastName":["Müller"],"initials":["P"],"affiliation":["University of Basel, Petersplatz 1, 4003, Basel, Switzerland."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["304"],"abstractText":["The Asian tiger mosquito, Aedes albopictus, is a highly invasive mosquito species of public health importance. In the wake of its arrival in neighbouring Italy the authorities of the canton of Ticino in southern Switzerland initiated a surveillance programme in 2000 that is still on-going. Here we explored the unique data set, compiled from 2003 to 2014, to analyse the local dynamic of introduction and establishment of Ae. albopictus, its relative density in relation to precipitation and temperature, and its potential distribution at the passage from southern to northern Europe.The presence of Ae. albopictus was recorded by ovitraps placed across Ticino. In addition to presence-absence, the relationship between relative egg densities and year, month, temperature and precipitation was analysed by a generalised linear mixed model.Since its first detection in 2003 at Ticino's border with Italy Ae. albopictus has continuously spread north across the lower valleys, mainly along the trans-European motorway, E35. Detailed local analysis showed that industrial areas were colonised by the mosquito before residential areas and that, afterwards, the mosquito was more present in residential than in industrial areas. Ae. albopictus appeared sporadically and then became more present in the same places the following years, suggesting gradual establishment of locally reproducing populations that manage to overwinter. This trend continues as witnessed by both a growing area being infested and increasing egg counts in the ovitraps. There was a clear South-North gradient with more traps being repeatedly positive in the South and fewer eggs laid during periods of intensive precipitation. In the North, the mosquito appeared repeatedly through the years, but never managed to establish, probably because of unfavourable weather conditions and low road traffic.Given the present results we assume that additional areas may still become infested. While the current study provides good estimates of relative egg densities and shows the local and regional dynamics of Ae. albopictus invasion, additional parameters ought to be measured to make an objective risk assessment for epidemic disease transmission. The likelihood of Ae. albopictus to further spread and increase in densities calls for continued surveillance."],"affiliation":["Laboratory of Eco-Epidemiology of Parasites, Institute of Biology, University of Neuchâtel, Emile-Argand 11, 2000, Neuchâtel, Switzerland. eleonora.flacio@supsi.ch."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882898?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882898"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1577-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-27"],"dateOfRevision":["2016-05-29"],"electronicPublicationDate":["2016-05-26"],"firstPublicationDate":["2016-05-26"],"cprojectID":"PMC4882898"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhwJ9hGurOzZIau","_score":1,"_source":{"id":["27333276"],"source":["MED"],"pmid":["27333276"],"pmcid":["PMC4917172"],"doi":["10.1371/journal.pntd.0004758"],"title":["Spatial and Temporal Hot Spots of Aedes albopictus Abundance inside and outside a South European Metropolitan Area."],"authorString":["Manica M, Filipponi F, D'Alessandro A, Screti A, Neteler M, Rosà R, Solimini A, Della Torre A, Caputo B."],"authorList":[{"author":[{"fullName":["Manica M"],"firstName":["Mattia"],"lastName":["Manica"],"initials":["M"],"affiliation":["Dipartimento di Biodiversità ed Ecologia Molecolare, Centro Ricerca e Innovazione, Fondazione Edmund Mach, San Michele all'Adige, Trentino, Italy."]},{"fullName":["Filipponi F"],"firstName":["Federico"],"lastName":["Filipponi"],"initials":["F"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]},{"fullName":["D'Alessandro A"],"firstName":["Antonello"],"lastName":["D'Alessandro"],"initials":["A"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]},{"fullName":["Screti A"],"firstName":["Alessia"],"lastName":["Screti"],"initials":["A"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]},{"fullName":["Neteler M"],"firstName":["Markus"],"lastName":["Neteler"],"initials":["M"],"affiliation":["mundialis GmbH & Co. KG, Bonn, Germany."]},{"fullName":["Rosà R"],"firstName":["Roberto"],"lastName":["Rosà"],"initials":["R"],"authorId":[{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}],"affiliation":["Dipartimento di Biodiversità ed Ecologia Molecolare, Centro Ricerca e Innovazione, Fondazione Edmund Mach, San Michele all'Adige, Trentino, Italy."]},{"fullName":["Solimini A"],"firstName":["Angelo"],"lastName":["Solimini"],"initials":["A"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]},{"fullName":["Della Torre A"],"firstName":["Alessandra"],"lastName":["Della Torre"],"initials":["A"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]},{"fullName":["Caputo B"],"firstName":["Beniamino"],"lastName":["Caputo"],"initials":["B"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Piazzale Aldo Moro 5, Rome, Italy."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004758"],"abstractText":["Aedes albopictus is a tropical invasive species which in the last decades spread worldwide, also colonizing temperate regions of Europe and US, where it has become a public health concern due to its ability to transmit exotic arboviruses, as well as severe nuisance problems due to its aggressive daytime outdoor biting behaviour. While several studies have been carried out in order to predict the potential limits of the species expansions based on eco-climatic parameters, few studies have so far focused on the specific effects of these variables in shaping its micro-geographic abundance and dynamics. The present study investigated eco-climatic factors affecting Ae. albopictus abundance and dynamics in metropolitan and sub-urban/rural sites in Rome (Italy), which was colonized in 1997 and is nowadays one of the most infested metropolitan areas in Southern Europe. To this aim, longitudinal adult monitoring was carried out along a 70 km-transect across and beyond the most urbanized and densely populated metropolitan area. Two fine scale spatiotemporal datasets (one with reference to a 20m circular buffer around sticky traps used to collect mosquitoes and the second to a 300m circular buffer within each sampling site) were exploited to analyze the effect of climatic and socio-environmental variables on Ae. albopictus abundance and dynamics along the transect. Results showed an association between highly anthropized habitats and high adult abundance both in metropolitan and sub-urban/rural areas, with \"small green islands\" corresponding to hot spots of abundance in the metropolitan areas only, and a bimodal seasonal dynamics with a second peak of abundance in autumn, due to heavy rains occurring in the preceding weeks in association with permissive temperatures. The results provide useful indications to prioritize public mosquito control measures in temperate urban areas where nuisance, human-mosquito contact and risk of local arbovirus transmission are likely higher, and highlight potential public health risks also after the summer months typically associated with high mosquito densities."],"affiliation":["Dipartimento di Biodiversità ed Ecologia Molecolare, Centro Ricerca e Innovazione, Fondazione Edmund Mach, San Michele all'Adige, Trentino, Italy."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917172?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917172"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004758"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-23"],"dateOfRevision":["2016-07-09"],"electronicPublicationDate":["2016-06-22"],"firstPublicationDate":["2016-06-22"],"cprojectID":"PMC4917172"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrk-hOUS2orL1IQ_","_score":1,"_source":{"id":["27334012"],"source":["MED"],"pmid":["27334012"],"pmcid":["PMC4917985"],"doi":["10.1186/s12974-016-0628-1"],"title":["IL-33 signaling is essential to attenuate viral-induced encephalitis development by downregulating iNOS expression in the central nervous system."],"authorString":["Franca RF, Costa RS, Silva JR, Peres RS, Mendonça LR, Colón DF, Alves-Filho JC, Cunha FQ."],"authorList":[{"author":[{"fullName":["Franca RF"],"firstName":["Rafael F O"],"lastName":["Franca"],"initials":["RF"],"affiliation":["Department of Virology and Experimental Therapy LAVITE, Oswaldo Cruz Foundation - FIOCRUZ, Institute Aggeu Magalhães IAM, Av. Professor Moraes Rego, s/n, Recife, PE, 50740-465, Brazil. rafael.franca@cpqam.fiocruz.br."]},{"fullName":["Costa RS"],"firstName":["Renata S"],"lastName":["Costa"],"initials":["RS"],"affiliation":["Program of Basic and Applied Immunology, University of São Paulo, Ribeirao Preto, SP, 14049-900, Brazil."]},{"fullName":["Silva JR"],"firstName":["Jaqueline R"],"lastName":["Silva"],"initials":["JR"],"affiliation":["Program of Basic and Applied Immunology, University of São Paulo, Ribeirao Preto, SP, 14049-900, Brazil."]},{"fullName":["Peres RS"],"firstName":["Raphael S"],"lastName":["Peres"],"initials":["RS"],"affiliation":["Program of Basic and Applied Immunology, University of São Paulo, Ribeirao Preto, SP, 14049-900, Brazil."]},{"fullName":["Mendonça LR"],"firstName":["Leila R"],"lastName":["Mendonça"],"initials":["LR"],"affiliation":["Department of Virology and Experimental Therapy LAVITE, Oswaldo Cruz Foundation - FIOCRUZ, Institute Aggeu Magalhães IAM, Av. Professor Moraes Rego, s/n, Recife, PE, 50740-465, Brazil."]},{"fullName":["Colón DF"],"firstName":["David F"],"lastName":["Colón"],"initials":["DF"],"affiliation":["Program of Basic and Applied Immunology, University of São Paulo, Ribeirao Preto, SP, 14049-900, Brazil."]},{"fullName":["Alves-Filho JC"],"firstName":["José Carlos"],"lastName":["Alves-Filho"],"initials":["JC"],"affiliation":["Department of Pharmacology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil."]},{"fullName":["Cunha FQ"],"firstName":["Fernando Q"],"lastName":["Cunha"],"initials":["FQ"],"affiliation":["Department of Pharmacology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil. fdqcunha@fmrp.uspbr."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0417-779X","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["13"],"journalIssueId":["2358851"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of neuroinflammation"],"ISOAbbreviation":["J Neuroinflammation"],"medlineAbbreviation":["J Neuroinflammation"],"NLMid":["101222974"],"ESSN":["1742-2094"]}]}],"pubYear":["2016"],"pageInfo":["159"],"abstractText":["Viral encephalitis is a common cause of lethal infections in humans, and several different viruses are documented to be responsible. Rocio virus is a flavivirus that causes a severe lethal encephalitis syndrome in humans and also mice, providing an interesting model to study the CNS compartmentalized immune response. Interleukin 33 (IL-33), a member of the IL-1 family, is an immunomodulatory cytokine that is highly expressed in the CNS. However, the role of IL-33 on viral encephalitis remains unclear. Therefore, we aimed to explore how the IL-33/ST2 axis regulates the local immune response during Rocio virus infection.Wild-type (WT), ST2 (ST2(-/-)), and nitric oxide synthase-deficient mice (iNOS(-/-)) and Stat6 (Stat6(-/-))-deficient mice were infected with different concentrations of the Rocio virus by intraperitoneal route, the cytokine mRNA level in CNS was analyzed by qPCR, and cellular immunophenotyping was performed on infected mice by the flow cytometry of isolated CNS mononuclear cells.We have shown that the mRNA expression of IL-33 and ST2 receptors is increased in the CNS of Rocio virus-infected WT mice and that ST2(-/-) mice showed increased susceptibility to infection. ST2 deficiency was correlated with increased tissue pathology, cellular infiltration, and tumor necrosis factor alpha (TNF-α) and interferon-gamma (IFN-γ) mRNA levels and higher viral load in the CNS, compared with wild-type mice. The increased Th1 cytokine levels released in the CNS acted on infiltrating macrophages, as evidenced by flow cytometry characterization of cellular infiltrates, inducing the expression of iNOS, contributing to brain injury. Moreover, iNOS(-/-) mice were more resistant to Rocio virus encephalitis, presenting a lower clinical score and reduced mortality rate, despite the increased tissue pathology.We provide evidences of a specific role for IL-33 receptor signaling in nitric oxide induction through local IFN-γ modulation, suggesting that nitric oxide overproduction might have an important role in the progression of experimental viral encephalitis."],"affiliation":["Department of Virology and Experimental Therapy LAVITE, Oswaldo Cruz Foundation - FIOCRUZ, Institute Aggeu Magalhães IAM, Av. Professor Moraes Rego, s/n, Recife, PE, 50740-465, Brazil. rafael.franca@cpqam.fiocruz.br."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Nitric oxide","Flavivirus","Interleukin 33","Experimental Viral Encephalitis","St2 Receptor"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917985?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917985"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12974-016-0628-1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-23"],"dateOfRevision":["2016-06-26"],"electronicPublicationDate":["2016-06-22"],"firstPublicationDate":["2016-06-22"],"cprojectID":"PMC4917985"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrk_hOUS2orL1IRA","_score":1,"_source":{"id":["27184629"],"source":["MED"],"pmid":["27184629"],"pmcid":["PMC4918162"],"doi":["10.3201/eid2207.160326"],"title":["Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses."],"authorString":["Waggoner JJ, Gresh L, Mohamed-Hadley A, Ballesteros G, Davila MJ, Tellez Y, Sahoo MK, Balmaseda A, Harris E, Pinsky BA."],"authorList":[{"author":[{"fullName":["Waggoner JJ"],"firstName":["Jesse J"],"lastName":["Waggoner"],"initials":["JJ"]},{"fullName":["Gresh L"],"firstName":["Lionel"],"lastName":["Gresh"],"initials":["L"]},{"fullName":["Mohamed-Hadley A"],"firstName":["Alisha"],"lastName":["Mohamed-Hadley"],"initials":["A"]},{"fullName":["Ballesteros G"],"firstName":["Gabriela"],"lastName":["Ballesteros"],"initials":["G"]},{"fullName":["Davila MJ"],"firstName":["Maria Jose Vargas"],"lastName":["Davila"],"initials":["MJ"]},{"fullName":["Tellez Y"],"firstName":["Yolanda"],"lastName":["Tellez"],"initials":["Y"]},{"fullName":["Sahoo MK"],"firstName":["Malaya K"],"lastName":["Sahoo"],"initials":["MK"]},{"fullName":["Balmaseda A"],"firstName":["Angel"],"lastName":["Balmaseda"],"initials":["A"]},{"fullName":["Harris E"],"firstName":["Eva"],"lastName":["Harris"],"initials":["E"]},{"fullName":["Pinsky BA"],"firstName":["Benjamin A"],"lastName":["Pinsky"],"initials":["BA"]}]}],"journalInfo":[{"issue":["7"],"volume":["22"],"journalIssueId":["2397213"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1295-1297"],"abstractText":["Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Viruses","Dengue virus","Arboviruses","Chikungunya virus","real-time RT-PCR","Vector-borne Infections","Zika Virus","Multiplex Molecular Diagnostics"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918162?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918162"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2207.160326"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"embargoDate":["2016-07-01"],"cprojectID":"PMC4918162"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrop_8VqgcuJwvjD","_score":1,"_source":{"id":["27322537"],"source":["MED"],"pmid":["27322537"],"pmcid":["PMC4913923"],"doi":["10.1371/journal.pone.0157120"],"title":["Seasonal Differences in Density But Similar Competitive Impact of Aedes albopictus (Skuse) on Aedes aegypti (L.) in Rio de Janeiro, Brazil."],"authorString":["Camara DC, Codeço CT, Juliano SA, Lounibos LP, Riback TI, Pereira GR, Honorio NA."],"authorList":[{"author":[{"fullName":["Camara DC"],"firstName":["Daniel Cardoso Portela"],"lastName":["Camara"],"initials":["DC"],"affiliation":["Núcleo Operacional Sentinela de Mosquitos Vetores - NOSMOVE, DIRAC - IOC - VPAAPS, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Codeço CT"],"firstName":["Claudia Torres"],"lastName":["Codeço"],"initials":["CT"],"affiliation":["Programa de Computação Científica, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Juliano SA"],"firstName":["Steven A"],"lastName":["Juliano"],"initials":["SA"],"affiliation":["School of Biological Sciences, Behavior, Ecology, Evolution and Systematics Section, Illinois State University, Normal Illinois, United States of America."]},{"fullName":["Lounibos LP"],"firstName":["L Philip"],"lastName":["Lounibos"],"initials":["LP"],"affiliation":["Florida Medical Entomology Laboratory, University of Florida, Vero Beach Florida, United States of America."]},{"fullName":["Riback TI"],"firstName":["Thais Irene Souza"],"lastName":["Riback"],"initials":["TI"],"affiliation":["Programa de Computação Científica, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Pereira GR"],"firstName":["Glaucio Rocha"],"lastName":["Pereira"],"initials":["GR"],"affiliation":["Núcleo Operacional Sentinela de Mosquitos Vetores - NOSMOVE, DIRAC - IOC - VPAAPS, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Honorio NA"],"firstName":["Nildimar Alves"],"lastName":["Honorio"],"initials":["NA"],"affiliation":["Núcleo Operacional Sentinela de Mosquitos Vetores - NOSMOVE, DIRAC - IOC - VPAAPS, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."]}]}],"journalInfo":[{"issue":["6"],"volume":["11"],"journalIssueId":["2413805"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0157120"],"abstractText":["Previous studies have shown that the negative effects of density of Ae. albopictus on Ae. aegypti exceed those of Ae. aegypti on Ae. albopictus for population growth, adult size, survivorship, and developmental rate. This competitive superiority has been invoked to explain the displacement of Ae. aegypti by Ae. albopictus in the southeastern USA. In Brazil, these species coexist in many vegetated suburban and rural areas. We investigated a related, but less-well-studied question: do effects of Ae. albopictus on Ae. aegypti larval development and survival occur under field conditions at realistic densities across multiple seasons in Brazil? We conducted additive competition experiments in a vegetated area of Rio de Janeiro where these species coexist. We tested the hypothesis that Ae. aegypti (the focal species, at a fixed density) suffers negative effects on development and survivorship across a gradient of increasing densities of Ae. albopictus (the associate species) in three seasons. The results showed statistically significant effects of both season and larval density on Ae. aegypti survivorship, and significant effects of season on development rate, with no significant season-density interactions. Densities of Aedes larvae in these habitats differed among seasons by a factor of up to 7x. Overall, Spring was the most favorable season for Ae. aegypti survivorship and development. Results showed that under natural conditions the negative competitive effects of Ae. albopictus on Ae. aegypti were expressed primarily as lower survivorship. Coexistence between Ae. aegypti and Ae. albopictus in vegetated areas is likely affected by seasonal environmental differences, such as detrital resource levels or egg desiccation, which can influence competition between these species. Interactions between these Aedes are important in Brazil, where both species are well established and widely distributed and vector dengue, Zika and chikungunya viruses."],"affiliation":["Núcleo Operacional Sentinela de Mosquitos Vetores - NOSMOVE, DIRAC - IOC - VPAAPS, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4913923?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4913923"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0157120"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-21"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-20"],"firstPublicationDate":["2016-06-20"],"cprojectID":"PMC4913923"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnroo_8VqgcuJwvjC","_score":1,"_source":{"id":["27306056"],"source":["MED"],"pmid":["27306056"],"pmcid":["PMC4910250"],"doi":["10.1186/s12985-016-0556-9"],"title":["Detection of chikungunya virus in saliva and urine."],"authorString":["Musso D, Teissier A, Rouault E, Teururai S, de Pina JJ, Nhan TX."],"authorList":[{"author":[{"fullName":["Musso D"],"firstName":["Didier"],"lastName":["Musso"],"initials":["D"],"affiliation":["Pôle de recherche et de veille sur les maladies infectieuses émergentes, Institut Louis Malardé, Tahiti, French Polynesia. dmusso@ilm.pf."]},{"fullName":["Teissier A"],"firstName":["Anita"],"lastName":["Teissier"],"initials":["A"],"affiliation":["Pôle de recherche et de veille sur les maladies infectieuses émergentes, Institut Louis Malardé, Tahiti, French Polynesia."]},{"fullName":["Rouault E"],"firstName":["Eline"],"lastName":["Rouault"],"initials":["E"],"affiliation":["Laboratoire de biologie médicale, Institut Louis Malardé, PO Box 30, 98713, Papeete, Tahiti, French Polynesia."]},{"fullName":["Teururai S"],"firstName":["Sylviane"],"lastName":["Teururai"],"initials":["S"],"affiliation":["Laboratoire de biologie médicale, Institut Louis Malardé, PO Box 30, 98713, Papeete, Tahiti, French Polynesia."]},{"fullName":["de Pina JJ"],"firstName":["Jean-Jacques"],"lastName":["de Pina"],"initials":["JJ"],"affiliation":["Laboratoire de biologie médicale, Institut Louis Malardé, PO Box 30, 98713, Papeete, Tahiti, French Polynesia."]},{"fullName":["Nhan TX"],"firstName":["Tu-Xuan"],"lastName":["Nhan"],"initials":["TX"],"affiliation":["Laboratoire de biologie médicale, Institut Louis Malardé, PO Box 30, 98713, Papeete, Tahiti, French Polynesia."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2401989"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["102"],"abstractText":["Saliva and urine have been used for arthropod-borne viruses molecular detection but not yet for chikungunya virus (CHIKV). We investigated the use of saliva and urine for molecular detection of CHIKV during the French Polynesian outbreak.During the French Polynesian chikungunya outbreak (2014-2015), we collected the same day blood and saliva samples from 60 patients with probable chikungunya (47 during the 1st week post symptoms onset and 13 after), urine was available for 39 of them. All samples were tested using a CHIKV reverse-transcription PCR.Forty eight patients had confirmed chikungunya. For confirmed chikungunya presenting during the 1st week post symptoms onset, CHIKV RNA was detected from 86.1 % (31/36) of blood, 58.3 % (21/36) of saliva and 8.3 % (2/24) of urine. Detection rate of CHIKV RNA was significantly higher in blood compared to saliva. For confirmed chikungunya presenting after the 1st week post symptoms onset, CHIKV RNA was detected from 8.3 % (1/12) of blood, 8.3 % (1/12) of saliva and 0 % (0/8) of urine.In contrast to Zika virus (ZIKV), saliva did not increased the detection rate of CHIKV RNA during the 1st week post symptoms onset. In contrast to ZIKV, dengue virus and West Nile virus, urine did not enlarged the window of detection of CHIKV RNA after the 1st week post symptoms onset. Saliva can be used for molecular detection of CHIKV during the 1st week post symptoms onset only if blood is impossible to collect but with a lower sensitivity compared to blood."],"affiliation":["Pôle de recherche et de veille sur les maladies infectieuses émergentes, Institut Louis Malardé, Tahiti, French Polynesia. dmusso@ilm.pf."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Saliva","Urine","PCR","Arbovirus","French Polynesia","Chikungunya","Chikv"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4910250?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4910250"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0556-9"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27377265"],"source":["MED"],"reference":["Virol J. 2016;13(1):120"],"type":["Erratum in"],"orderIn":["1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-16"],"dateOfRevision":["2016-06-18"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4910250"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrpM_8VqgcuJwvjG","_score":1,"_source":{"id":["27191188"],"source":["MED"],"pmid":["27191188"],"pmcid":["PMC4918159"],"doi":["10.3201/eid2207.160022"],"title":["Development of Medical Countermeasures to Middle East Respiratory Syndrome Coronavirus."],"authorString":["Uyeki TM, Erlandson KJ, Korch G, O'Hara M, Wathen M, Hu-Primmer J, Hojvat S, Stemmy EJ, Donabedian A."],"authorList":[{"author":[{"fullName":["Uyeki TM"],"firstName":["Timothy M"],"lastName":["Uyeki"],"initials":["TM"]},{"fullName":["Erlandson KJ"],"firstName":["Karl J"],"lastName":["Erlandson"],"initials":["KJ"]},{"fullName":["Korch G"],"firstName":["George"],"lastName":["Korch"],"initials":["G"]},{"fullName":["O'Hara M"],"firstName":["Michael"],"lastName":["O'Hara"],"initials":["M"]},{"fullName":["Wathen M"],"firstName":["Michael"],"lastName":["Wathen"],"initials":["M"]},{"fullName":["Hu-Primmer J"],"firstName":["Jean"],"lastName":["Hu-Primmer"],"initials":["J"]},{"fullName":["Hojvat S"],"firstName":["Sally"],"lastName":["Hojvat"],"initials":["S"]},{"fullName":["Stemmy EJ"],"firstName":["Erik J"],"lastName":["Stemmy"],"initials":["EJ"]},{"fullName":["Donabedian A"],"firstName":["Armen"],"lastName":["Donabedian"],"initials":["A"]}]}],"journalInfo":[{"issue":["7"],"volume":["22"],"journalIssueId":["2397213"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"abstractText":["Preclinical development of and research on potential Middle East respiratory syndrome coronavirus (MERS-CoV) medical countermeasures remain preliminary; advancements are needed before most countermeasures are ready to be tested in human clinical trials. Research priorities include standardization of animal models and virus stocks for studying disease pathogenesis and efficacy of medical countermeasures; development of MERS-CoV diagnostics; improved access to nonhuman primates to support preclinical research; studies to better understand and control MERS-CoV disease, including vaccination studies in camels; and development of a standardized clinical trial protocol. Partnering with clinical trial networks in affected countries to evaluate safety and efficacy of investigational therapeutics will strengthen efforts to identify successful medical countermeasures."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Antibodies","Vaccines","Viruses","Monoclonal","Non-human primates","Therapeutics","Nonhuman primates","Animal Models","Respiratory Infections","Diagnostics","Antivirals","polyclonal","Medical Countermeasures","Mers-cov","Middle East Respiratory Syndrome Coronavirus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918159"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2207.160022"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"embargoDate":["2016-07-01"],"cprojectID":"PMC4918159"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrphhOUS2orL1IRX","_score":1,"_source":{"id":["27389584"],"source":["MED"],"pmid":["27389584"],"pmcid":["PMC4936676"],"doi":["10.1371/journal.ppat.1005715"],"title":["Metarhizium brunneum Blastospore Pathogenesis in Aedes aegypti Larvae: Attack on Several Fronts Accelerates Mortality."],"authorString":["Alkhaibari AM, Carolino AT, Yavasoglu SI, Maffeis T, Mattoso TC, Bull JC, Samuels RI, Butt TM."],"authorList":[{"author":[{"fullName":["Alkhaibari AM"],"firstName":["Abeer M"],"lastName":["Alkhaibari"],"initials":["AM"],"affiliation":["Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, United Kingdom."]},{"fullName":["Carolino AT"],"firstName":["Aline T"],"lastName":["Carolino"],"initials":["AT"],"affiliation":["Department of Entomology and Plant Pathology, State University of North Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil."]},{"fullName":["Yavasoglu SI"],"firstName":["Sare I"],"lastName":["Yavasoglu"],"initials":["SI"],"affiliation":["Department of Biology, Faculty of Arts & Sciences, Adnan Menderes University, Aydin, Turkey."]},{"fullName":["Maffeis T"],"firstName":["Thierry"],"lastName":["Maffeis"],"initials":["T"],"affiliation":["College of Engineering, Swansea University, Swansea, United Kingdom."]},{"fullName":["Mattoso TC"],"firstName":["Thalles C"],"lastName":["Mattoso"],"initials":["TC"],"affiliation":["Department of Entomology and Plant Pathology, State University of North Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil."]},{"fullName":["Bull JC"],"firstName":["James C"],"lastName":["Bull"],"initials":["JC"],"affiliation":["Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, United Kingdom."]},{"fullName":["Samuels RI"],"firstName":["Richard I"],"lastName":["Samuels"],"initials":["RI"],"affiliation":["Department of Entomology and Plant Pathology, State University of North Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil."]},{"fullName":["Butt TM"],"firstName":["Tariq M"],"lastName":["Butt"],"initials":["TM"],"affiliation":["Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, United Kingdom."]}]}],"journalInfo":[{"issue":["7"],"volume":["12"],"journalIssueId":["2424238"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005715"],"abstractText":["Aedes aegypti is the vector of a wide range of diseases (e.g. yellow fever, dengue, Chikungunya and Zika) which impact on over half the world's population. Entomopathogenic fungi such as Metarhizium anisopliae and Beauveria bassiana have been found to be highly efficacious in killing mosquito larvae but only now are the underlying mechanisms for pathogenesis being elucidated. Recently it was shown that conidia of M. anisopliae caused stress induced mortality in Ae. aegypti larvae, a different mode of pathogenicity to that normally seen in terrestrial hosts. Blastospores constitute a different form of inoculum produced by this fungus when cultured in liquid media and although blastospores are generally considered to be more virulent than conidia no evidence has been presented to explain why. In our study, using a range of biochemical, molecular and microscopy methods, the infection process of Metarhizium brunneum (formerly M. anisopliae) ARSEF 4556 blastospores was investigated. It appears that the blastospores, unlike conidia, readily adhere to and penetrate mosquito larval cuticle. The blastospores are readily ingested by the larvae but unlike the conidia are able infect the insect through the gut and rapidly invade the haemocoel. The fact that pathogenicity related genes were upregulated in blastospores exposed to larvae prior to invasion, suggests the fungus was detecting host derived cues. Similarly, immune and defence genes were upregulated in the host prior to infection suggesting mosquitoes were also able to detect pathogen-derived cues. The hydrophilic blastospores produce copious mucilage, which probably facilitates adhesion to the host but do not appear to depend on production of Pr1, a cuticle degrading subtilisin protease, for penetration since protease inhibitors did not significantly alter blastospore virulence. The fact the blastospores have multiple routes of entry (cuticle and gut) may explain why this form of the inoculum killed Ae. aegypti larvae in a relatively short time (12-24hrs), significantly quicker than when larvae were exposed to conidia. This study shows that selecting the appropriate form of inoculum is important for efficacious control of disease vectors such as Ae. aegypti."],"affiliation":["Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4936676?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4936676"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005715"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-08"],"dateOfRevision":["2016-07-24"],"electronicPublicationDate":["2016-07-07"],"firstPublicationDate":["2016-07-07"],"cprojectID":"PMC4936676"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrp7hOUS2orL1IRZ","_score":1,"_source":{"id":["27353760"],"source":["MED"],"pmid":["27353760"],"pmcid":["PMC4937217"],"doi":["10.1128/mbio.00907-16"],"title":["The Brazilian Dilemma: Increased Scientific Production and High Publication Costs during a Global Health Crisis and Major Economic Downturn."],"authorString":["Rodrigues ML, Morel CM."],"authorList":[{"author":[{"fullName":["Rodrigues ML"],"firstName":["Marcio L"],"lastName":["Rodrigues"],"initials":["ML"],"authorId":[{"_":"0000-0002-6081-3439","$":{"type":"ORCID"}}],"affiliation":["Fundação Oswaldo Cruz (Fiocruz), Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro, Brazil Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil marciolrodrig@gmail.com morel@cdts.fiocruz.br."]},{"fullName":["Morel CM"],"firstName":["Carlos M"],"lastName":["Morel"],"initials":["CM"],"affiliation":["Fundação Oswaldo Cruz (Fiocruz), Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro, Brazil marciolrodrig@gmail.com morel@cdts.fiocruz.br."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6081-3439","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["7"],"journalIssueId":["2404410"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"affiliation":["Fundação Oswaldo Cruz (Fiocruz), Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Rio de Janeiro, Brazil Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil marciolrodrig@gmail.com morel@cdts.fiocruz.br."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4937217?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4937217"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.00907-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-29"],"dateOfRevision":["2016-07-13"],"electronicPublicationDate":["2016-06-28"],"firstPublicationDate":["2016-06-28"],"cprojectID":"PMC4937217"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrra_8VqgcuJwvjR","_score":1,"_source":{"id":["27366587"],"source":["MED"],"pmid":["27366587"],"pmcid":["PMC4922883"],"doi":["10.1371/currents.outbreaks.711379ace737b7c04c89765342a9a8c9"],"title":["FLIRT-ing with Zika: A Web Application to Predict the Movement of Infected Travelers Validated Against the Current Zika Virus Epidemic."],"authorString":["Huff A, Allen T, Whiting K, Breit N, Arnold B."],"authorList":[{"author":[{"fullName":["Huff A"],"firstName":["Andrew"],"lastName":["Huff"],"initials":["A"]},{"fullName":["Allen T"],"firstName":["Toph"],"lastName":["Allen"],"initials":["T"],"affiliation":["Technology & Data Science, EcoHealth Alliance, New York, NY, USA."]},{"fullName":["Whiting K"],"firstName":["Karissa"],"lastName":["Whiting"],"initials":["K"],"affiliation":["Technology & Data Science, EcoHealth Alliance, New York, NY, USA."]},{"fullName":["Breit N"],"firstName":["Nathan"],"lastName":["Breit"],"initials":["N"],"affiliation":["Technology & Data Science, EcoHealth Alliance, New York, NY, USA."]},{"fullName":["Arnold B"],"firstName":["Brock"],"lastName":["Arnold"],"initials":["B"],"affiliation":["Technology & Data Science, EcoHealth Alliance, New York, NY, USA."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":[" Beginning in 2015, Zika virus rapidly spread throughout the Americas and has been linked to neurological and autoimmune diseases in adults and babies. Developing accurate tools to anticipate Zika spread is one of the first steps to mitigate further spread of the disease. When combined, air traffic data and network simulations can be used to create tools to predict where infectious disease may spread to and aid in the prevention of infectious diseases. Specific goals were to: 1) predict where travelers infected with the Zika Virus would arrive in the U.S.; and, 2) analyze and validate the open access web application's (i.e., FLIRT) predictions using data collected after the prediction was made.FLIRT was built to predict the flow and likely destinations of infected travelers through the air travel network. FLIRT uses a database of flight schedules from over 800 airlines, and can display direct flight traffic and perform passenger simulations between selected airports. FLIRT was used to analyze flights departing from five selected airports in locations where sustained Zika Virus transmission was occurring. FLIRT's predictions were validated against Zika cases arriving in the U.S. from selected airports during the selected time periods. Kendall's τ and Generalized Linear Models were computed for all permutations of FLIRT and case data to test the accuracy of FLIRT's predictions.FLIRT was found to be predictive of the final destinations of infected travelers in the U.S. from areas with ongoing transmission of Zika in the Americas from 01 February 2016 - 01 to April 2016, and 11 January 2016 to 11 March 2016 time periods. MIA-FLL, JFK-EWR-LGA, and IAH were top ranked at-risk metro areas, and Florida, Texas and New York were top ranked states at-risk for the future time period analyzed (11 March 2016 - 11 June 2016). For the 11 January 2016 to 11 March 2016 time period, the region-aggregated model indicated 7.24 (95% CI 6.85 - 7.62) imported Zika cases per 100,000 passengers, and the state-aggregated model suggested 11.33 (95% CI 10.80 - 11.90) imported Zika cases per 100,000 passengers.The results from 01 February 2016 to 01 April 2016 and 11 January 2016 to 11 March 2016 time periods support that modeling air travel and passenger movement can be a powerful tool in predicting where infectious diseases will spread next. As FLIRT was shown to significantly predict distribution of Zika Virus cases in the past, there should be heightened biosurveillance and educational campaigns to medical service providers and the general public in these states, especially in the large metropolitan areas."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Networks","Air Travel","Flirt","Zika Virus","Disease Forecasting","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4922883"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.711379ace737b7c04c89765342a9a8c9"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-01"],"dateOfCreation":["2016-07-01"],"electronicPublicationDate":["2016-06-10"],"firstPublicationDate":["2016-06-10"],"cprojectID":"PMC4922883"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrsJJ9hGurOzZIbS","_score":1,"_source":{"id":["27416771"],"source":["MED"],"pmid":["27416771"],"pmcid":["PMC4946210"],"doi":["10.1186/s13071-016-1634-y"],"title":["Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population."],"authorString":["Bona AC, Chitolina RF, Fermino ML, de Castro Poncio L, Weiss A, Lima JB, Paldi N, Bernardes ES, Henen J, Maori E."],"authorList":[{"author":[{"fullName":["Bona AC"],"firstName":["Ana Caroline Dalla"],"lastName":["Bona"],"initials":["AC"],"affiliation":["Forrest Brasil Tecnologia Ltda, Curitiba, PR, Brazil."]},{"fullName":["Chitolina RF"],"firstName":["Rodrigo Faitta"],"lastName":["Chitolina"],"initials":["RF"],"affiliation":["Forrest Brasil Tecnologia Ltda, Curitiba, PR, Brazil."]},{"fullName":["Fermino ML"],"firstName":["Marise Lopes"],"lastName":["Fermino"],"initials":["ML"],"affiliation":["Forrest Brasil Tecnologia Ltda, Curitiba, PR, Brazil."]},{"fullName":["de Castro Poncio L"],"firstName":["Lisiane"],"lastName":["de Castro Poncio"],"initials":["L"],"affiliation":["Forrest Brasil Tecnologia Ltda, Curitiba, PR, Brazil."]},{"fullName":["Weiss A"],"firstName":["Avital"],"lastName":["Weiss"],"initials":["A"],"affiliation":["Forrest Innovations Ltd, Caesarea, Israel."]},{"fullName":["Lima JB"],"firstName":["José Bento Pereira"],"lastName":["Lima"],"initials":["JB"],"affiliation":["Instituto Oswaldo Cruz - Fiocruz, Laboratório de Fisiologia e Controle de artrópodes vetores, Rio de Janeiro, RJ, Brazil."]},{"fullName":["Paldi N"],"firstName":["Nitzan"],"lastName":["Paldi"],"initials":["N"],"affiliation":["Forrest Innovations Ltd, Caesarea, Israel."]},{"fullName":["Bernardes ES"],"firstName":["Emerson Soares"],"lastName":["Bernardes"],"initials":["ES"],"affiliation":["Nuclear Energy Research Institute, Radiopharmacy Center, São Paulo, Brazil."]},{"fullName":["Henen J"],"firstName":["Jonathan"],"lastName":["Henen"],"initials":["J"],"affiliation":["Forrest Innovations Ltd, Caesarea, Israel."]},{"fullName":["Maori E"],"firstName":["Eyal"],"lastName":["Maori"],"initials":["E"],"authorId":[{"_":"0000-0002-9541-9554","$":{"type":"ORCID"}}],"affiliation":["Forrest Innovations Ltd, Caesarea, Israel. Eyal@forrestinnovations.com."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9541-9554","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["397"],"abstractText":["Mosquitoes host and pass on to humans a variety of disease-causing pathogens such as infectious viruses and other parasitic microorganisms. The emergence and spread of insecticide resistance is threatening the effectiveness of current control measures for common mosquito vector borne diseases, such as malaria, dengue and Zika. Therefore, the emerging resistance to the widely used pyrethroid insecticides is an alarming problem for public health. Herein we demonstrated the use of RNA interference (RNAi) to increase susceptibility of adult mosquitoes to a widely used pyrethroid insecticide.Experiments were performed on a field-collected pyrethroid resistant strain of Ae. aegypti (Rio de Janeiro; RJ). Larvae from the resistant Ae. aegypti population were soaked with double-stranded RNAs (dsRNAs) that correspond either to voltage-gate sodium channel (VGSC), P-glycoprotein, or P450 detoxification genes and reared to adulthood. Adult mortality rates in the presence of various Deltamethrin pyrethroid concentrations were used to assess mosquito insecticide susceptibility.We characterized the RJ Ae. aegypti strain with regard to its level of resistance to a pyrethroid insecticide and found that it was approximately 6 times more resistant to Deltamethrin compared to the laboratory Rockefeller strain. The RJ strain displayed a higher frequency of Val1016Ile and Phe1534Cys substitutions of the VGSC gene. The resistant strain also displayed a higher basal expression level of VGSC compared to the Rockefeller strain. When dsRNA-treated mosquitoes were subjected to a standard pyrethroid contact bioassay, only dsRNA targeting VGSC increased the adult mortality of the pyrethroid resistant strain. The dsRNA treatment proved effective in increasing adult mosquito susceptibility over a range of pyrethroid concentrations and these results were associated with dsRNA-specific small interfering RNAs in treated adults, and the corresponding specific down regulation of VGSC gene expression level. Finally, we demonstrated that the efficiency of our approach was further improved by 'tiling' along the VGSC gene in order to identify the most potent dsRNA sequences.These results demonstrate that dsRNA applied to mosquito larvae retains its biological activity into adulthood. Thus, the RNAi system reported here could be a useful approach to control the widespread insecticide resistance in mosquitoes and other insect vectors of human diseases."],"affiliation":["Forrest Brasil Tecnologia Ltda, Curitiba, PR, Brazil."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Pyrethroids","Aedes aegypti","Insecticide resistance","RNAi","Mosquitoes","voltage-gated sodium channel"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4946210?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4946210"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1634-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-07-14"],"firstPublicationDate":["2016-07-14"],"cprojectID":"PMC4946210"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrs7J9hGurOzZIbV","_score":1,"_source":{"id":["27333366"],"source":["MED"],"pmid":["27333366"],"pmcid":["PMC4957500"],"doi":["10.1590/0074-02760160134"],"title":["Larvicidal efficacies of plants from Midwestern Brazil: melianodiol from Guarea kunthiana as a potential biopesticide against Aedes aegypti."],"authorString":["Sarmento UC, Miguita CH, Almeida LH, Gaban CR, Silva LM, Souza AS, Garcez WS, Garcez FR."],"authorList":[{"author":[{"fullName":["Sarmento UC"],"firstName":["Ulana Chaves"],"lastName":["Sarmento"],"initials":["UC"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Miguita CH"],"firstName":["Carlos Henrique"],"lastName":["Miguita"],"initials":["CH"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Almeida LH"],"firstName":["Luís Henrique de Oliveira"],"lastName":["Almeida"],"initials":["LH"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Gaban CR"],"firstName":["Cleusa Rocha Garcia"],"lastName":["Gaban"],"initials":["CR"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Silva LM"],"firstName":["Lilliam May Grespan Estodutto da"],"lastName":["Silva"],"initials":["LM"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Souza AS"],"firstName":["Albert Schiaveto de"],"lastName":["Souza"],"initials":["AS"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Centro de Ciências Biológicas e da Saúde, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Centro de Ciências Biológicas e da Saúde, Campo Grande, MS, Brasil."]},{"fullName":["Garcez WS"],"firstName":["Walmir Silva"],"lastName":["Garcez"],"initials":["WS"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]},{"fullName":["Garcez FR"],"firstName":["Fernanda Rodrigues"],"lastName":["Garcez"],"initials":["FR"],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."]}]}],"journalInfo":[{"volume":["0"],"journalIssueId":["2417578"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["0"],"abstractText":["A total of 36 ethanol extracts from different anatomical parts of 27 plant species (18 families), native to the Pantanal and Cerrado biomes in Midwest Brazil, was assessed for their effect against Aedes aegypti larvae, the vector of dengue, hemorrhagic dengue, Zika and chikungunya fevers. Only the extract obtained from seeds of Guarea kunthiana (Meliaceae) proved active (LC50 = 169.93 μg/mL). A bioassay-guided investigation of this extract led to the isolation and identification of melianodiol, a protolimonoid, as the active constituent (LC50 = 14.44 mg/mL). Meliantriol, which was also obtained from the bioactive fraction, was nevertheless devoid of any larval toxicity, even at the highest concentration tested (LC50 > 100.0 mg/mL). These results indicate that the larvicidal activity of melianodiol stems from the presence of the carbonyl moiety at C-3 in the 21,23-epoxy-21,24,25-trihydroxy-tirucall-7-ene-type skeleton. The structures of both protolimonoids were established on the basis of spectral methods (1H and 13C NMR and MS). This is the first report on the toxicity of melianodiol against Ae. aegypti larvae. Based on the results, melianodiol can be regarded as a potential candidate for use as an ecologically sound biocontrol agent for reducing the larval population of this vector."],"affiliation":["Universidade Federal de Mato Grosso do Sul, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande MS , Brasil, Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, MS, Brasil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957500?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957500"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160134"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-23"],"dateOfRevision":["2016-07-28"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4957500"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrvvhOUS2orL1IRx","_score":1,"_source":{"id":["27540468"],"source":["MED"],"pmid":["27540468"],"pmcid":["PMC4965698"],"doi":["10.12688/f1000research.8853.1"],"title":["MEPPitope: spatial, electrostatic and secondary structure perturbations in the post-fusion Dengue virus envelope protein highlights known epitopes and conserved residues in the Zika virus."],"authorString":["Chakraborty S."],"authorList":[{"author":[{"fullName":["Chakraborty S"],"firstName":["Sandeep"],"lastName":["Chakraborty"],"initials":["S"],"affiliation":["Celia Engineers, Navi Mumbai, India."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["1150"],"abstractText":["The dramatic transformation of the Zika virus (ZIKV) from a relatively unknown virus to a pathogen generating global-wide panic has exposed the dearth of detailed knowledge about this virus. Decades of research in the related Dengue virus (DENV), finally culminating in a vaccine registered for use in endemic regions (CYD-TDV), provides key insights in developing strategies for tackling ZIKV. The previously established MEPP methodology compares two conformations of the same protein and identifies residues with significant spatial and electrostatic perturbations. In the current work, MEPP analyzed the pre-and post-fusion DENV type 2 envelope (E) protein, and identified several known epitopes (His317, Tyr299, Glu26, Arg188, etc.) (MEPPitope). These residues are overwhelmingly conserved in ZIKV and all DENV serotypes. Characterization of α-helices in E-proteins show that α1 is not conserved in the sequence space of ZIKV and DENV. Furthermore, perturbation of α1 in the post-fusion DENV structure includes a known epitope Asp215, a residue absent in the pre-fusion α1. A cationic β-sheet in the GAG-binding domain that is stereochemically equivalent in ZIKV and all DENV serotypes is also highlighted due to a residue pair (Arg286-Arg288) that has a significant electrostatic polarity reversal upon fusion. Finally, two highly conserved residues (Thr32 and Thr40), with little emphasis in existing literature, are found to have significant electrostatic perturbation. Thus, a combination of different computational methods enable the rapid and rational detection of critical residues that can be made the target of small drugs, or as epitopes in the search for an elusive therapy or vaccine that neutralizes multiple members of the Flaviviridae family."],"affiliation":["Celia Engineers, Navi Mumbai, India."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Epitopes","Envelope proteins","Guillain-Barre syndrome","Dengue","Microcephaly","Neutralizing Antibodies","Flaviviruses","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4965698?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4965698"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8853.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"," doi"," "]}],"dateOfCompletion":["2016-08-19"],"dateOfCreation":["2016-08-19"],"dateOfRevision":["2016-08-21"],"electronicPublicationDate":["2016-06-03"],"firstPublicationDate":["2016-06-03"],"cprojectID":"PMC4965698"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwLJ9hGurOzZIbh","_score":1,"_source":{"id":["27464886"],"source":["MED"],"pmid":["27464886"],"pmcid":["PMC4963997"],"doi":["10.1186/s13071-016-1705-0"],"title":["Storm drains as larval development and adult resting sites for Aedes aegypti and Aedes albopictus in Salvador, Brazil."],"authorString":["Paploski IA, Rodrigues MS, Mugabe VA, Kikuti M, Tavares AS, Reis MG, Kitron U, Ribeiro GS."],"authorList":[{"author":[{"fullName":["Paploski IA"],"firstName":["Igor Adolfo Dexheimer"],"lastName":["Paploski"],"initials":["IA"],"affiliation":["Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, BA, Brazil."]},{"fullName":["Rodrigues MS"],"firstName":["Moreno S"],"lastName":["Rodrigues"],"initials":["MS"],"affiliation":["Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, BA, Brazil."]},{"fullName":["Mugabe VA"],"firstName":["Vánio André"],"lastName":["Mugabe"],"initials":["VA"],"affiliation":["Universidade Pedagógica de Quelimane, Quelimane, ZB, Mozambique."]},{"fullName":["Kikuti M"],"firstName":["Mariana"],"lastName":["Kikuti"],"initials":["M"],"affiliation":["Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, BA, Brazil."]},{"fullName":["Tavares AS"],"firstName":["Aline S"],"lastName":["Tavares"],"initials":["AS"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Saúde, Salvador, BA, Brazil."]},{"fullName":["Reis MG"],"firstName":["Mitermayer Galvão"],"lastName":["Reis"],"initials":["MG"],"affiliation":["Faculdade de Medicina, Universidade Federal da Bahia, Salvador, BA, Brazil."]},{"fullName":["Kitron U"],"firstName":["Uriel"],"lastName":["Kitron"],"initials":["U"],"affiliation":["Emory University, Atlanta, GE, USA."]},{"fullName":["Ribeiro GS"],"firstName":["Guilherme Sousa"],"lastName":["Ribeiro"],"initials":["GS"],"affiliation":["Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, BA, Brazil. guilherme.ribeiro@bahia.fiocruz.br."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["419"],"abstractText":["Dengue (DENV), Chikungunya (CHIKV), Zika (ZIKV), as well as yellow fever (YFV) viruses are transmitted to humans by Aedes spp. females. In Salvador, the largest urban center in north-eastern Brazil, the four DENV types have been circulating, and more recently, CHIKV and ZIKV have also become common. We studied the role of storm drains as Aedes larval development and adult resting sites in four neighbourhoods of Salvador, representing different socioeconomic, infrastructure and topographic conditions.A sample of 122 storm drains in the four study sites were surveyed twice during a 4-month period in 2015; in 49.0 % of the visits, the storm drains contained water. Adults and immatures of Aedes aegypti were captured in two of the four sites, and adults and immatures of Aedes albopictus were captured in one of these two sites. A total of 468 specimens were collected: 148 Ae. aegypti (38 adults and 110 immatures), 79 Ae. albopictus (48 adults and 31 immatures), and 241 non-Aedes (mainly Culex spp.) mosquitoes (42 adults and 199 immatures). The presence of adults or immatures of Ae. aegypti in storm drains was independently associated with the presence of non-Aedes mosquitoes and with rainfall of ≤ 50 mm during the preceding week.We found that in Salvador, one of the epicentres of the 2015 ZIKV outbreak, storm drains often accumulate water and serve as larval development sites and adult resting areas for both Ae. aegypti and Ae. albopictus. Vector control campaigns usually overlook storm drains, as most of the effort to prevent Ae. agypti reproduction is directed towards containers in the domicile environment. While further studies are needed to determine the added contribution of storm drains for the maintenance of Aedes spp. populations, we advocate that vector control programs incorporate actions directed at storm drains, including regular inspections and use of larvicides, and that human and capital resources are mobilized to modify storm drains, so that they do not serves as larval development sites for Aedes (and other) mosquitoes."],"affiliation":["Instituto de Saúde Coletiva, Universidade Federal da Bahia, Salvador, BA, Brazil."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Entomology","Arboviruses","Insect vectors","epidemiology","Disease vectors"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963997?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963997"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1705-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-28"],"dateOfRevision":["2016-07-31"],"electronicPublicationDate":["2016-07-27"],"firstPublicationDate":["2016-07-27"],"cprojectID":"PMC4963997"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwLJ9hGurOzZIbi","_score":1,"_source":{"id":["27354505"],"source":["MED"],"pmid":["27354505"],"pmcid":["PMC4966430"],"doi":["10.1177/1757913916650225"],"title":["Time to abandon the hygiene hypothesis: new perspectives on allergic disease, the human microbiome, infectious disease prevention and the role of targeted hygiene."],"authorString":["Bloomfield SF, Rook GA, Scott EA, Shanahan F, Stanwell-Smith R, Turner P."],"authorList":[{"author":[{"fullName":["Bloomfield SF"],"firstName":["Sally F"],"lastName":["Bloomfield"],"initials":["SF"],"affiliation":["London School of Hygiene & Tropical Medicine and International Scientific Forum on Home Hygiene, The Old Dairy Cottage, Montacute, Somerset TA15 6XL, UK sallyfbloomfield@aol.com."]},{"fullName":["Rook GA"],"firstName":["Graham Aw"],"lastName":["Rook"],"initials":["GA"],"affiliation":["Centre for Clinical Microbiology, Department of Infection, University College London (UCL), London, UK."]},{"fullName":["Scott EA"],"firstName":["Elizabeth A"],"lastName":["Scott"],"initials":["EA"],"affiliation":["Center for Hygiene and Health, Department of Biology, Simmons College, Boston, MA, USA."]},{"fullName":["Shanahan F"],"firstName":["Fergus"],"lastName":["Shanahan"],"initials":["F"],"affiliation":["APC Microbiome Institute, University College Cork - National University of Ireland, Cork, Ireland."]},{"fullName":["Stanwell-Smith R"],"firstName":["Rosalind"],"lastName":["Stanwell-Smith"],"initials":["R"],"affiliation":["London School of Hygiene & Tropical Medicine, London, UK."]},{"fullName":["Turner P"],"firstName":["Paul"],"lastName":["Turner"],"initials":["P"],"affiliation":["Section of Paediatrics (Allergy & Infectious Diseases) and MRC & Asthma UK Centre in Allergic Mechanisms of Asthma, Imperial College London, London, UK; Discipline of Paediatrics and Child Health, The University of Sydney, Sydney, NSW, Australia."]}]}],"journalInfo":[{"issue":["4"],"volume":["136"],"journalIssueId":["2423652"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Perspectives in public health"],"ISOAbbreviation":["Perspect Public Health"],"medlineAbbreviation":["Perspect Public Health"],"NLMid":["101499631"],"ISSN":["1757-9139"],"ESSN":["1757-9147"]}]}],"pubYear":["2016"],"pageInfo":["213-224"],"abstractText":["To review the burden of allergic and infectious diseases and the evidence for a link to microbial exposure, the human microbiome and immune system, and to assess whether we could develop lifestyles which reconnect us with exposures which could reduce the risk of allergic disease while also protecting against infectious disease.Using methodology based on the Delphi technique, six experts in infectious and allergic disease were surveyed to allow for elicitation of group judgement and consensus view on issues pertinent to the aim.Key themes emerged where evidence shows that interaction with microbes that inhabit the natural environment and human microbiome plays an essential role in immune regulation. Changes in lifestyle and environmental exposure, rapid urbanisation, altered diet and antibiotic use have had profound effects on the human microbiome, leading to failure of immunotolerance and increased risk of allergic disease. Although evidence supports the concept of immune regulation driven by microbe-host interactions, the term 'hygiene hypothesis' is a misleading misnomer. There is no good evidence that hygiene, as the public understands, is responsible for the clinically relevant changes to microbial exposures.Evidence suggests a combination of strategies, including natural childbirth, breast feeding, increased social exposure through sport, other outdoor activities, less time spent indoors, diet and appropriate antibiotic use, may help restore the microbiome and perhaps reduce risks of allergic disease. Preventive efforts must focus on early life. The term 'hygiene hypothesis' must be abandoned. Promotion of a risk assessment approach (targeted hygiene) provides a framework for maximising protection against pathogen exposure while allowing spread of essential microbes between family members. To build on these findings, we must change public, public health and professional perceptions about the microbiome and about hygiene. We need to restore public understanding of hygiene as a means to prevent infectious disease."],"affiliation":["London School of Hygiene & Tropical Medicine and International Scientific Forum on Home Hygiene, The Old Dairy Cottage, Montacute, Somerset TA15 6XL, UK sallyfbloomfield@aol.com."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Antibiotics","Allergy","Cleaning","Diet","Infectious disease","Hygiene"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4966430?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4966430"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1177/1757913916650225"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-29"],"dateOfRevision":["2016-08-14"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4966430"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzJJ9hGurOzZIbt","_score":1,"_source":{"id":["27396345"],"source":["MED"],"pmid":["27396345"],"pmcid":["PMC4972564"],"doi":["10.1016/j.ebiom.2016.06.035"],"title":["Mice are not Men: ADAM30 Findings Emphasize a Broader Look Towards Murine Alzheimer's Disease Models."],"authorString":["Becker-Pauly C, Pietrzik CU."],"authorList":[{"author":[{"fullName":["Becker-Pauly C"],"firstName":["Christoph"],"lastName":["Becker-Pauly"],"initials":["C"],"affiliation":["Unit for Degradomics of the Protease Web, Institute of Biochemistry, University of Kiel, 24118 Kiel, Germany. Electronic address: cbeckerpauly@biochem.uni-kiel.de."]},{"fullName":["Pietrzik CU"],"firstName":["Claus U"],"lastName":["Pietrzik"],"initials":["CU"],"affiliation":["Institute for Pathobiochemistry, University Medical Center of the Johannes Gutenberg University of Mainz, 55128 Mainz, Germany. Electronic address: pietrzik@uni-mainz.de."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2435137"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["19-20"],"affiliation":["Unit for Degradomics of the Protease Web, Institute of Biochemistry, University of Kiel, 24118 Kiel, Germany. Electronic address: cbeckerpauly@biochem.uni-kiel.de."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972564?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972564"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.06.035"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-01"],"dateOfRevision":["2016-08-12"],"electronicPublicationDate":["2016-06-25"],"firstPublicationDate":["2016-06-25"],"cprojectID":"PMC4972564"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrleJ9hGurOzZIa1","_score":1,"_source":{"id":["27332734"],"source":["MED"],"pmid":["27332734"],"pmcid":["PMC4920956"],"doi":["10.1016/j.immuni.2016.06.002"],"title":["Host Inflammatory Response to Mosquito Bites Enhances the Severity of Arbovirus Infection."],"authorString":["Pingen M, Bryden SR, Pondeville E, Schnettler E, Kohl A, Merits A, Fazakerley JK, Graham GJ, McKimmie CS."],"authorList":[{"author":[{"fullName":["Pingen M"],"firstName":["Marieke"],"lastName":["Pingen"],"initials":["M"],"affiliation":["Virus Host Interaction Team, Section of Infection and Immunity, Leeds Institute of Cancer and Pathology, University of Leeds, Leeds LS9 7TF, UK."]},{"fullName":["Bryden SR"],"firstName":["Steven R"],"lastName":["Bryden"],"initials":["SR"],"affiliation":["Virus Host Interaction Team, Section of Infection and Immunity, Leeds Institute of Cancer and Pathology, University of Leeds, Leeds LS9 7TF, UK; Institute of Infection, Immunology and Inflammation, University of Glasgow, Glasgow G12 8TA, UK."]},{"fullName":["Pondeville E"],"firstName":["Emilie"],"lastName":["Pondeville"],"initials":["E"],"affiliation":["MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK."]},{"fullName":["Schnettler E"],"firstName":["Esther"],"lastName":["Schnettler"],"initials":["E"],"affiliation":["MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK."]},{"fullName":["Kohl A"],"firstName":["Alain"],"lastName":["Kohl"],"initials":["A"],"affiliation":["MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, UK."]},{"fullName":["Merits A"],"firstName":["Andres"],"lastName":["Merits"],"initials":["A"],"affiliation":["Institute of Technology, University of Tartu, 50411 Tartu, Estonia."]},{"fullName":["Fazakerley JK"],"firstName":["John K"],"lastName":["Fazakerley"],"initials":["JK"],"affiliation":["The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK."]},{"fullName":["Graham GJ"],"firstName":["Gerard J"],"lastName":["Graham"],"initials":["GJ"],"affiliation":["Institute of Infection, Immunology and Inflammation, University of Glasgow, Glasgow G12 8TA, UK."]},{"fullName":["McKimmie CS"],"firstName":["Clive S"],"lastName":["McKimmie"],"initials":["CS"],"authorId":[{"_":"0000-0002-7694-9509","$":{"type":"ORCID"}}],"affiliation":["Virus Host Interaction Team, Section of Infection and Immunity, Leeds Institute of Cancer and Pathology, University of Leeds, Leeds LS9 7TF, UK. Electronic address: c.s.mckimmie@leeds.ac.uk."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7694-9509","$":{"type":"ORCID"}},{"_":"0000-0001-5689-9076","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["44"],"journalIssueId":["2422149"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Immunity"],"ISOAbbreviation":["Immunity"],"medlineAbbreviation":["Immunity"],"NLMid":["9432918"],"ISSN":["1074-7613"],"ESSN":["1097-4180"]}]}],"pubYear":["2016"],"pageInfo":["1455-1469"],"abstractText":["Aedes aegypti mosquitoes are responsible for transmitting many medically important viruses such as those that cause Zika and dengue. The inoculation of viruses into mosquito bite sites is an important and common stage of all mosquito-borne virus infections. We show, using Semliki Forest virus and Bunyamwera virus, that these viruses use this inflammatory niche to aid their replication and dissemination in vivo. Mosquito bites were characterized by an edema that retained virus at the inoculation site and an inflammatory influx of neutrophils that coordinated a localized innate immune program that inadvertently facilitated virus infection by encouraging the entry and infection of virus-permissive myeloid cells. Neutrophil depletion and therapeutic blockade of inflammasome activity suppressed inflammation and abrogated the ability of the bite to promote infection. This study identifies facets of mosquito bite inflammation that are important determinants of the subsequent systemic course and clinical outcome of virus infection."],"affiliation":["Virus Host Interaction Team, Section of Infection and Immunity, Leeds Institute of Cancer and Pathology, University of Leeds, Leeds LS9 7TF, UK."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920956"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.immuni.2016.06.002"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-23"],"dateOfRevision":["2016-07-01"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4920956"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmoJ9hGurOzZIa7","_score":1,"_source":{"id":["PMC4928527"],"source":["PMC"],"pmcid":["PMC4928527"],"title":["Vaccination guidelines in patients with chronic kidney disease and renal transplant recipients travelling abroad."],"journalInfo":[{"issue":["Suppl 1"],"volume":["26"],"journalIssueId":["2428903"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Indian journal of nephrology"],"ISOAbbreviation":["Indian J Nephrol"],"medlineAbbreviation":["Indian J Nephrol"],"NLMid":["8914356"],"ISSN":["0971-4065"],"ESSN":["1998-3662"]}]}],"pubYear":["2016"],"pageInfo":["S26-S28"],"language":["eng"],"pubModel":["Undetermined"],"pubTypeList":[{"pubType":["Abstract"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928527"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.org/articles/PMC4928527/?tool=EBI"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-13"],"dateOfRevision":["2016-07-13"],"firstPublicationDate":["2016-04-01"],"cprojectID":"PMC4928527"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmzJ9hGurOzZIa8","_score":1,"_source":{"id":["27364935"],"source":["MED"],"pmid":["27364935"],"pmcid":["PMC4929456"],"doi":["10.1038/srep28792"],"title":["The wMel strain of Wolbachia Reduces Transmission of Zika virus by Aedes aegypti."],"authorString":["Aliota MT, Peinado SA, Velez ID, Osorio JE."],"authorList":[{"author":[{"fullName":["Aliota MT"],"firstName":["Matthew T"],"lastName":["Aliota"],"initials":["MT"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA."]},{"fullName":["Peinado SA"],"firstName":["Stephen A"],"lastName":["Peinado"],"initials":["SA"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA."]},{"fullName":["Velez ID"],"firstName":["Ivan Dario"],"lastName":["Velez"],"initials":["ID"],"affiliation":["Programa de Estudio y Control de Enfermedades Tropicales (PECET), Universidad de Antioquia, Medellin, A1226, Colombia."]},{"fullName":["Osorio JE"],"firstName":["Jorge E"],"lastName":["Osorio"],"initials":["JE"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["28792"],"abstractText":["Zika virus (ZIKV) is causing an explosive outbreak of febrile disease in the Americas. There are no effective antiviral therapies or licensed vaccines for this virus, and mosquito control strategies have not been adequate to contain the virus. A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV) transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against other arboviruses. At present, it is unknown whether or not ZIKV can infect, disseminate, and be transmitted by Wolbachia-infected Ae. aegypti. Using Ae. aegypti infected with the wMel strain of Wolbachia that are being released in Medellin, Colombia, we report that these mosquitoes have reduced vector competence for ZIKV. These results support the use of Wolbachia biocontrol as a multivalent strategy against Ae. aegypti-transmitted viruses."],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4929456?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4929456"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep28792"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-07-01"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-07-01"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4929456"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnron_8VqgcuJwvjB","_score":1,"_source":{"id":["27304097"],"source":["MED"],"pmid":["27304097"],"pmcid":["PMC4909043"],"doi":["10.1590/0074-02760160003"],"title":["Zika is not a reason for missing the Olympic Games in Rio de Janeiro: response to the open letter of Dr Attaran and colleagues to Dr Margaret Chan, Director - General, WHO, on the Zika threat to the Olympic and Paralympic Games."],"authorString":["Codeço C, Villela D, Gomes MF, Bastos L, Cruz O, Struchiner C, Carvalho LM, Coelho F."],"authorList":[{"author":[{"fullName":["Codeço C"],"firstName":["Claudia"],"lastName":["Codeço"],"initials":["C"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Villela D"],"firstName":["Daniel"],"lastName":["Villela"],"initials":["D"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Gomes MF"],"firstName":["Marcelo F"],"lastName":["Gomes"],"initials":["MF"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Bastos L"],"firstName":["Leonardo"],"lastName":["Bastos"],"initials":["L"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Cruz O"],"firstName":["Oswaldo"],"lastName":["Cruz"],"initials":["O"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Struchiner C"],"firstName":["Claudio"],"lastName":["Struchiner"],"initials":["C"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Carvalho LM"],"firstName":["Luis Max"],"lastName":["Carvalho"],"initials":["LM"],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Coelho F"],"firstName":["Flavio"],"lastName":["Coelho"],"initials":["F"],"affiliation":["Fundação Getúlio Vargas, Fundação Getúlio Vargas, Rio de Janeiro RJ , Brasil, Fundação Getúlio Vargas, Rio de Janeiro, RJ, Brasil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-4693-5402","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["111"],"journalIssueId":["2420073"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["414-415"],"abstractText":["Attaran and colleagues in an open letter to WHO expressed their concern about the upcoming Olympic and Paralympic Games in Rio de Janeiro and the threat posed by the Zika epidemic (Attaran 2016). We agree that Zika virus is of great public health concern and much remains to be known about this disease. Care should be taken to reduce the risk of infection, especially to pregnant women. However, we argue that this is not sufficient reason for changing the original plans for the Games, in particular because of the time of the year when they will take place. The present article outlines several scientific results related to Zika and mosquito-borne infectious diseases dynamics that we believe ratify the current position of WHO in not endorsing the postponing or relocation of the 2016 Olympic and Paralympic Games (WHO 2016)."],"affiliation":["Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro RJ , Brasil, Fundação Oswaldo Cruz, Programa de Computação Científica, Rio de Janeiro, RJ, Brasil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909043?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909043"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160003"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-16"],"dateOfRevision":["2016-06-17"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4909043"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr0JhOUS2orL1IR9","_score":1,"_source":{"id":["27381219"],"source":["MED"],"pmid":["27381219"],"pmcid":["PMC4972906"],"doi":["10.1038/emi.2016.68"],"title":["Increasing genetic diversity of Zika virus in the Latin American outbreak."],"authorString":["Shi W, Zhang Z, Ling C, Carr MJ, Tong Y, Gao GF."],"authorList":[{"author":[{"fullName":["Shi W"],"firstName":["Weifeng"],"lastName":["Shi"],"initials":["W"],"affiliation":["Institute of Pathogen Biology, Taishan Medical College, Taian, Shandong Province 271000, China."]},{"fullName":["Zhang Z"],"firstName":["Zhenjie"],"lastName":["Zhang"],"initials":["Z"],"affiliation":["Institute of Pathogen Biology, Taishan Medical College, Taian, Shandong Province 271000, China."]},{"fullName":["Ling C"],"firstName":["Cheng"],"lastName":["Ling"],"initials":["C"],"affiliation":["College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China."]},{"fullName":["Carr MJ"],"firstName":["Michael J"],"lastName":["Carr"],"initials":["MJ"],"affiliation":["National Virus Reference Laboratory, University College Dublin, Dublin 4, Ireland."]},{"fullName":["Tong Y"],"firstName":["Yigang"],"lastName":["Tong"],"initials":["Y"],"affiliation":["State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100101, China."]},{"fullName":["Gao GF"],"firstName":["George F"],"lastName":["Gao"],"initials":["GF"],"affiliation":["Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2370343"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Emerging microbes & infections"],"ISOAbbreviation":["Emerg Microbes Infect"],"medlineAbbreviation":["Emerg Microbes Infect"],"NLMid":["101594885"],"ESSN":["2222-1751"]}]}],"pubYear":["2016"],"pageInfo":["e68"],"affiliation":["Institute of Pathogen Biology, Taishan Medical College, Taian, Shandong Province 271000, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972906?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972906"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/emi.2016.68"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-07-06"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-07-06"],"firstPublicationDate":["2016-07-06"],"cprojectID":"PMC4972906"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr26hOUS2orL1ISE","_score":1,"_source":{"id":["27498856"],"source":["MED"],"pmid":["27498856"],"pmcid":["PMC4976331"],"doi":["10.1038/srep31170"],"title":["Singapore grouper iridovirus protein VP088 is essential for viral infectivity."],"authorString":["Yuan Y, Wang Y, Liu Q, Zhu F, Hong Y."],"authorList":[{"author":[{"fullName":["Yuan Y"],"firstName":["Yongming"],"lastName":["Yuan"],"initials":["Y"],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."]},{"fullName":["Wang Y"],"firstName":["Yunzhi"],"lastName":["Wang"],"initials":["Y"],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."]},{"fullName":["Liu Q"],"firstName":["Qizhi"],"lastName":["Liu"],"initials":["Q"],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."]},{"fullName":["Zhu F"],"firstName":["Feng"],"lastName":["Zhu"],"initials":["F"],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."]},{"fullName":["Hong Y"],"firstName":["Yunhan"],"lastName":["Hong"],"initials":["Y"],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["31170"],"abstractText":["Viral infection is a great challenge in healthcare and agriculture. The Singapore grouper iridovirus (SGIV) is highly infectious to numerous marine fishes and increasingly threatens mariculture and wildlife conservation. SGIV intervention is not available because little is known about key players and their precise roles in SGVI infection. Here we report the precise role of VP088 as a key player in SGIV infection. VP088 was verified as an envelope protein encoded by late gene orf088. We show that SGIV could be neutralized with an antibody against VP088. Depletion or deletion of VP088 significantly suppresses SGIV infection without altering viral gene expression and host responses. By precisely quantifying the genome copy numbers of host cells and virions, we reveal that VP088 deletion dramatically reduces SGIV infectivity through inhibiting virus entry without altering viral pathogenicity, genome stability and replication and progeny virus release. These results pinpoint that VP088 is a key player in SGIV entry and represents an ideal target for SGIV intervention."],"affiliation":["Department of Biological Sciences, National University of Singapore, Science Drive 4, Singapore 117543, Singapore."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976331?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976331"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep31170"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-08"],"dateOfRevision":["2016-08-23"],"electronicPublicationDate":["2016-08-08"],"firstPublicationDate":["2016-08-08"],"cprojectID":"PMC4976331"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3BJ9hGurOzZIb0","_score":1,"_source":{"id":["27336445"],"source":["MED"],"pmid":["27336445"],"pmcid":["PMC4974448"],"doi":["10.4178/epih.e2016026"],"title":["The current status of Zika virus in Southeast Asia."],"authorString":["Wiwanitkit V."],"authorList":[{"author":[{"fullName":["Wiwanitkit V"],"firstName":["Viroj"],"lastName":["Wiwanitkit"],"initials":["V"],"authorId":[{"_":"0000-0003-1039-3728","$":{"type":"ORCID"}}],"affiliation":["Department of Public Health Curriculum, Surindra Rajabhat University, Surin, Thailand."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-1039-3728","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["38"],"journalIssueId":["2430403"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Epidemiology and health"],"ISOAbbreviation":["Epidemiol Health"],"medlineAbbreviation":["Epidemiol Health"],"NLMid":["101519472"],"ESSN":["2092-7193"]}]}],"pubYear":["2016"],"pageInfo":["e2016026"],"abstractText":["Zika virus currently poses a global threat and is a major public health issue throughout Latin America and the Caribbean. However, Zika virus infections in humans have also been observed in other regions, including Southeast Asia, where arboviral diseases are very common. In this study, we summarize the current status of Zika virus in Southeast Asia. This review aims to provide an overview of the current situation and also to suggest ways of adequately managing the emergence of Zika virus in Southeast Asia.The literature searching for the reports on Zika virus in Southeast Asia was done using standard database PubMed and the re-analysis and summarization on the reports was done.A limited number of reports have addressed Zika virus disease in Southeast Asia, but it is has been confirmed that a problem already exists. Individual case reports and outbreaks of Zika virus have been confirmed in Southeast Asia. Several reports have also described patients becoming infected after visiting Southeast Asia. In addition, the concurrent circulation of Zika virus with other arboviruses has been confirmed.As a tropical region with a high prevalence of arboviral diseases, the emergence of Zika virus in Southeast Asia is a major concern. It is essential for local medical personnel to recognize this disease. Given the status of Southeast Asia as a globally important tourist destination, continuous updates on the status of Zika virus in Southeast Asia are required and should be incorporated into global health advisories regarding travel."],"affiliation":["Department of Public Health Curriculum, Surindra Rajabhat University, Surin, Thailand."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Infection","Southeast Asia","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974448?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974448"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4178/epih.e2016026"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-08-11"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4974448"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3W_8VqgcuJwvj8","_score":1,"_source":{"id":["27473856"],"source":["MED"],"pmid":["27473856"],"pmcid":["PMC4966872"],"doi":["10.1186/s12985-016-0590-7"],"title":["Flavivirus NS1: a multifaceted enigmatic viral protein."],"authorString":["Rastogi M, Sharma N, Singh SK."],"authorList":[{"author":[{"fullName":["Rastogi M"],"firstName":["Meghana"],"lastName":["Rastogi"],"initials":["M"],"affiliation":["Institute of Medical Sciences (IMS), Laboratory of Human Molecular Virology & Immunology, Molecular Biology Unit, Faculty of Medicine, Banaras Hindu University, Varanasi, 221005, India."]},{"fullName":["Sharma N"],"firstName":["Nikhil"],"lastName":["Sharma"],"initials":["N"],"affiliation":["Laboratory of Neurovirology and Inflammation Biology, CSIR-Centre for Cellular and Molecular Biology (CCMB), Uppal Road, Hyderabad, 500007, India."]},{"fullName":["Singh SK"],"firstName":["Sunit Kumar"],"lastName":["Singh"],"initials":["SK"],"affiliation":["Institute of Medical Sciences (IMS), Laboratory of Human Molecular Virology & Immunology, Molecular Biology Unit, Faculty of Medicine, Banaras Hindu University, Varanasi, 221005, India. sunitsingh2000@gmail.com."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2401989"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["131"],"abstractText":["Flaviviruses are emerging arthropod-borne viruses representing an immense global health problem. The prominent viruses of this group include dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus tick borne encephalitis virus and Zika Virus. These are endemic in many parts of the world. They are responsible for the illness ranging from mild flu like symptoms to severe hemorrhagic, neurologic and cognitive manifestations leading to death. NS1 is a highly conserved non-structural protein among flaviviruses, which exist in diverse forms. The intracellular dimer form of NS1 plays role in genome replication, whereas, the secreted hexamer plays role in immune evasion. The secreted NS1 has been identified as a potential diagnostic marker for early detection of the infections caused by flaviviruses. In addition to the diagnostic marker, the importance of NS1 has been reported in the development of therapeutics. NS1 based subunit vaccines are at various stages of development. The structural details and diverse functions of NS1 have been discussed in detail in this review."],"affiliation":["Institute of Medical Sciences (IMS), Laboratory of Human Molecular Virology & Immunology, Molecular Biology Unit, Faculty of Medicine, Banaras Hindu University, Varanasi, 221005, India."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["NS1 protein","Arboviruses","Flavivirus","Non-structural Proteins"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4966872?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4966872"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0590-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["sprot"]}],"dateOfCreation":["2016-07-30"],"dateOfRevision":["2016-08-01"],"electronicPublicationDate":["2016-07-29"],"firstPublicationDate":["2016-07-29"],"cprojectID":"PMC4966872"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr4XhOUS2orL1ISL","_score":1,"_source":{"id":["27509020"],"source":["MED"],"pmid":["27509020"],"pmcid":["PMC4980036"],"doi":["10.1371/journal.pntd.0004851"],"title":["Extended Evaluation of Virological, Immunological and Pharmacokinetic Endpoints of CELADEN: A Randomized, Placebo-Controlled Trial of Celgosivir in Dengue Fever Patients."],"authorString":["Sung C, Wei Y, Watanabe S, Lee HS, Khoo YM, Fan L, Rathore AP, Chan KW, Choy MM, Kamaraj US, Sessions OM, Aw P, de Sessions PF, Lee B, Connolly JE, Hibberd ML, Vijaykrishna D, Wijaya L, Ooi EE, Low JG, Vasudevan SG."],"authorList":[{"author":[{"fullName":["Sung C"],"firstName":["Cynthia"],"lastName":["Sung"],"initials":["C"],"affiliation":["Vigilance and Compliance Branch, Health Sciences Authority, Singapore."]},{"fullName":["Wei Y"],"firstName":["Yuan"],"lastName":["Wei"],"initials":["Y"],"affiliation":["Singapore Clinical Research Institute, Singapore."]},{"fullName":["Watanabe S"],"firstName":["Satoru"],"lastName":["Watanabe"],"initials":["S"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Lee HS"],"firstName":["How Sung"],"lastName":["Lee"],"initials":["HS"],"affiliation":["Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore."]},{"fullName":["Khoo YM"],"firstName":["Yok Moi"],"lastName":["Khoo"],"initials":["YM"],"affiliation":["Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore."]},{"fullName":["Fan L"],"firstName":["Lu"],"lastName":["Fan"],"initials":["L"],"affiliation":["Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore."]},{"fullName":["Rathore AP"],"firstName":["Abhay P S"],"lastName":["Rathore"],"initials":["AP"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Chan KW"],"firstName":["Kitti Wing-Ki"],"lastName":["Chan"],"initials":["KW"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Choy MM"],"firstName":["Milly M"],"lastName":["Choy"],"initials":["MM"],"affiliation":["Genome Institute of Singapore, A*STAR, Biopolis, Singapore."]},{"fullName":["Kamaraj US"],"firstName":["Uma S"],"lastName":["Kamaraj"],"initials":["US"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Sessions OM"],"firstName":["October M"],"lastName":["Sessions"],"initials":["OM"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Aw P"],"firstName":["Pauline"],"lastName":["Aw"],"initials":["P"],"affiliation":["Genome Institute of Singapore, A*STAR, Biopolis, Singapore."]},{"fullName":["de Sessions PF"],"firstName":["Paola F"],"lastName":["de Sessions"],"initials":["PF"],"affiliation":["Genome Institute of Singapore, A*STAR, Biopolis, Singapore."]},{"fullName":["Lee B"],"firstName":["Bernett"],"lastName":["Lee"],"initials":["B"],"affiliation":["Institute of Molecular & Cellular Biology, A*STAR, Biopolis, Singapore."]},{"fullName":["Connolly JE"],"firstName":["John E"],"lastName":["Connolly"],"initials":["JE"],"affiliation":["Institute of Molecular & Cellular Biology, A*STAR, Biopolis, Singapore."]},{"fullName":["Hibberd ML"],"firstName":["Martin L"],"lastName":["Hibberd"],"initials":["ML"],"affiliation":["Genome Institute of Singapore, A*STAR, Biopolis, Singapore."]},{"fullName":["Vijaykrishna D"],"firstName":["Dhanasekaran"],"lastName":["Vijaykrishna"],"initials":["D"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Wijaya L"],"firstName":["Limin"],"lastName":["Wijaya"],"initials":["L"],"affiliation":["Department of Infectious Diseases, Singapore General Hospital, Singapore."]},{"fullName":["Ooi EE"],"firstName":["Eng Eong"],"lastName":["Ooi"],"initials":["EE"],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]},{"fullName":["Low JG"],"firstName":["Jenny Guek-Hong"],"lastName":["Low"],"initials":["JG"],"affiliation":["Department of Infectious Diseases, Singapore General Hospital, Singapore."]},{"fullName":["Vasudevan SG"],"firstName":["Subhash G"],"lastName":["Vasudevan"],"initials":["SG"],"authorId":[{"_":"0000-0002-5083-3831","$":{"type":"ORCID"}}],"affiliation":["Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5083-3831","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004851"],"abstractText":["CELADEN was a randomized placebo-controlled trial of 50 patients with confirmed dengue fever to evaluate the efficacy and safety of celgosivir (A study registered at ClinicalTrials.gov, number NCT01619969). Celgosivir was given as a 400 mg loading dose and 200 mg bid (twice a day) over 5 days. Replication competent virus was measured by plaque assay and compared to reverse transcription quantitative PCR (qPCR) of viral RNA. Pharmacokinetics (PK) correlations with viremia, immunological profiling, next generation sequence (NGS) analysis and hematological data were evaluated as exploratory endpoints here to identify possible signals of pharmacological activity. Viremia by plaque assay strongly correlated with qPCR during the first four days. Immunological profiling demonstrated a qualitative shift in T helper cell profile during the course of infection. NGS analysis did not reveal any prominent signature that could be associated with drug treatment; however the phylogenetic spread of patients' isolates underlines the importance of strain variability that may potentially confound interpretation of dengue drug trials conducted during different outbreaks and in different countries. Celgosivir rapidly converted to castanospermine (Cast) with mean peak and trough concentrations of 5727 ng/mL (30.2 μM) and 430 ng/mL (2.3 μM), respectively and cleared with a half-life of 2.5 (± 0.6) hr. Mean viral log reduction between day 2 and 4 (VLR2-4) was significantly greater in secondary dengue than primary dengue (p = 0.002). VLR2-4 did not correlate with drug AUC but showed a trend of greater response with increasing Cmin. PK modeling identified dosing regimens predicted to achieve 2.4 to 4.5 times higher Cmin. than in the CELADEN trial for only 13% to 33% increase in overall dose. A small, non-statistical trend towards better outcome on platelet nadir and difference between maximum and minimum hematocrit was observed in celgosivir-treated patients with secondary dengue infection. Optimization of the dosing regimen and patient stratification may enhance the ability of a clinical trial to demonstrate celgosivir activity in treating dengue fever based on hematological endpoints. A new clinical trial with a revised dosing regimen is slated to start in 2016 (NCT02569827). Furthermore celgosivir's potential value for treatment of other flaviruses such as Zika virus should be investigated urgently.ClinicalTrials.gov NCT01619969."],"affiliation":["Vigilance and Compliance Branch, Health Sciences Authority, Singapore."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980036?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980036"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004851"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCreation":["2016-08-11"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-10"],"firstPublicationDate":["2016-08-10"],"cprojectID":"PMC4980036"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr4-_8VqgcuJwvj-","_score":1,"_source":{"id":["27482461"],"source":["MED"],"pmid":["27482461"],"pmcid":["PMC4967973"],"title":["Highlights from the 2016 International Symposium on HIV & Emerging Infectious Diseases (ISHEID): 25-27 May, Marseille, France."],"authorString":["Routy JP, Psomas C, Soriano V, Philibert P, Tissot-Dupont H, Lafeuillade A."],"authorList":[{"author":[{"fullName":["Routy JP"],"firstName":["Jean-Pierre"],"lastName":["Routy"],"initials":["JP"],"affiliation":["McGill Health Centre , Montreal , Canada."]},{"fullName":["Psomas C"],"firstName":["Christina"],"lastName":["Psomas"],"initials":["C"],"affiliation":["University Hospital , Montpellier , France."]},{"fullName":["Soriano V"],"firstName":["Vicente"],"lastName":["Soriano"],"initials":["V"],"affiliation":["Infectious Diseases Unit , La Paz University Hospital , Madrid , Spain."]},{"fullName":["Philibert P"],"firstName":["Patrick"],"lastName":["Philibert"],"initials":["P"],"affiliation":["European Hospital , Marseille , France."]},{"fullName":["Tissot-Dupont H"],"firstName":["Hervé"],"lastName":["Tissot-Dupont"],"initials":["H"],"affiliation":["Institut Hospitalo-Universitaire Méditerranée Infection , Marseille , France."]},{"fullName":["Lafeuillade A"],"firstName":["Alain"],"lastName":["Lafeuillade"],"initials":["A"],"affiliation":["General Hospital , Toulon , France."]}]}],"journalInfo":[{"issue":["3"],"volume":["2"],"journalIssueId":["2435853"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of virus eradication"],"ISOAbbreviation":["J Virus Erad"],"medlineAbbreviation":["J Virus Erad"],"NLMid":["101654142"],"ISSN":["2055-6640"],"ESSN":["2055-6659"]}]}],"pubYear":["2016"],"pageInfo":["187-192"],"abstractText":["For three days in May 2016, the International Symposium on HIV & Emerging Infectious Diseases gathered participants from all over the world around the theme 'Fighting deadly viruses'. HIV infection remained the main topic of the meeting but hepatitis, Ebola and Zika viruses as well as other emergent pathogens were also extensively covered. In this article we have tried to summarise what was presented during the plenary lectures, the two keynote lectures, and some of the work accepted for oral presentation. However, all abstracts can be found on the Journal of Virus Eradication website ( viruseradication.com/abstract.php)."],"affiliation":["McGill Health Centre , Montreal , Canada."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Tuberculosis","Hepatitis B","HIV","Hepatitis E","hepatitis C","Flu","Sexually Transmitted Infections","Hiv Vaccine","Ebola","Hiv Cure","Human Papilloma Viruses","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967973?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967973"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-04"],"electronicPublicationDate":["2016-07-01"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4967973"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr74_8VqgcuJwvkG","_score":1,"_source":{"id":["27516635"],"source":["MED"],"pmid":["27516635"],"pmcid":["PMC4969995"],"doi":["10.2471/blt.16.171207"],"title":["Background review for diagnostic test development for Zika virus infection."],"authorString":["Charrel RN, Leparc-Goffart I, Pas S, de Lamballerie X, Koopmans M, Reusken C."],"authorList":[{"author":[{"fullName":["Charrel RN"],"firstName":["Rémi N"],"lastName":["Charrel"],"initials":["RN"],"affiliation":["UMR EPV Emergence des Pathologies Virales, Aix Marseille Université, Marseille, France."]},{"fullName":["Leparc-Goffart I"],"firstName":["Isabelle"],"lastName":["Leparc-Goffart"],"initials":["I"],"affiliation":["French National Reference Centre for Arbovirus, Armed Forces Biomedical Research Institute, Marseille, France ."]},{"fullName":["Pas S"],"firstName":["Suzan"],"lastName":["Pas"],"initials":["S"],"affiliation":["Department of Viroscience, WHO Collaborating Centre for Arboviruses and Viral Haemorrhagic Fever Research and Reference, Wytemaweg 80, Room EE 17-26, 3015 CN Erasmus MC, Rotterdam, Netherlands ."]},{"fullName":["de Lamballerie X"],"firstName":["Xavier"],"lastName":["de Lamballerie"],"initials":["X"],"affiliation":["UMR EPV Emergence des Pathologies Virales, Aix Marseille Université, Marseille, France."]},{"fullName":["Koopmans M"],"firstName":["Marion"],"lastName":["Koopmans"],"initials":["M"],"affiliation":["Department of Viroscience, WHO Collaborating Centre for Arboviruses and Viral Haemorrhagic Fever Research and Reference, Wytemaweg 80, Room EE 17-26, 3015 CN Erasmus MC, Rotterdam, Netherlands ."]},{"fullName":["Reusken C"],"firstName":["Chantal"],"lastName":["Reusken"],"initials":["C"],"affiliation":["Department of Viroscience, WHO Collaborating Centre for Arboviruses and Viral Haemorrhagic Fever Research and Reference, Wytemaweg 80, Room EE 17-26, 3015 CN Erasmus MC, Rotterdam, Netherlands ."]}]}],"journalInfo":[{"issue":["8"],"volume":["94"],"journalIssueId":["2440780"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["574-584D"],"abstractText":["To review the state of knowledge about diagnostic testing for Zika virus infection and identify areas of research needed to address the current gaps in knowledge.We made a non-systematic review of the published literature about Zika virus and supplemented this with information from commercial diagnostic test kits and personal communications with researchers in European preparedness networks. The review covered current knowledge about the geographical spread, pathogen characteristics, life cycle and infection kinetics of the virus. The available molecular and serological tests and biosafety issues are described and discussed in the context of the current outbreak strain.We identified the following areas of research to address current knowledge gaps: (i) an urgent assessment of the laboratory capacity and capability of countries to detect Zika virus; (ii) rapid and extensive field validation of the available molecular and serological tests in areas with and without Zika virus transmission, with a focus on pregnant women; (iii) monitoring the genomic diversity of circulating Zika virus strains; (iv) prospective studies into the virus infection kinetics, focusing on diagnostic sampling (specimen types, combinations and timings); and (v) developing external quality assessments for molecular and serological testing, including differential diagnosis for similar viruses and symptom clusters. The availability of reagents for diagnostic development (virus strains and antigens, quantified viral ribonucleic acid) needs to be facilitated.An international laboratory response is needed, including preparation of protocols for prospective studies to address the most pressing information needs."],"affiliation":["UMR EPV Emergence des Pathologies Virales, Aix Marseille Université, Marseille, France."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969995?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969995"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.171207"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-12"],"dateOfRevision":["2016-08-17"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4969995"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8ohOUS2orL1ISb","_score":1,"_source":{"id":["27533717"],"source":["MED"],"pmid":["27533717"],"pmcid":["PMC4994370"],"doi":["10.3201/eid2209.160603"],"title":["Autochthonous Chikungunya Fever in Traveler Returning to Japan from Cuba."],"authorString":["Tsuboi M, Kutsuna S, Kato Y, Nakayama E, Shibasaki K, Tajima S, Takasaki T, Katanami Y, Yamamoto K, Takeshita N, Hayakawa K, Kanagawa S, Ohmagari N."],"authorList":[{"author":[{"fullName":["Tsuboi M"],"firstName":["Motoyuki"],"lastName":["Tsuboi"],"initials":["M"]},{"fullName":["Kutsuna S"],"firstName":["Satoshi"],"lastName":["Kutsuna"],"initials":["S"]},{"fullName":["Kato Y"],"firstName":["Yasuyuki"],"lastName":["Kato"],"initials":["Y"]},{"fullName":["Nakayama E"],"firstName":["Eri"],"lastName":["Nakayama"],"initials":["E"]},{"fullName":["Shibasaki K"],"firstName":["Ken-Ichi"],"lastName":["Shibasaki"],"initials":["K"]},{"fullName":["Tajima S"],"firstName":["Shigeru"],"lastName":["Tajima"],"initials":["S"]},{"fullName":["Takasaki T"],"firstName":["Tomohiko"],"lastName":["Takasaki"],"initials":["T"]},{"fullName":["Katanami Y"],"firstName":["Yuichi"],"lastName":["Katanami"],"initials":["Y"]},{"fullName":["Yamamoto K"],"firstName":["Kei"],"lastName":["Yamamoto"],"initials":["K"]},{"fullName":["Takeshita N"],"firstName":["Nozomi"],"lastName":["Takeshita"],"initials":["N"]},{"fullName":["Hayakawa K"],"firstName":["Kayoko"],"lastName":["Hayakawa"],"initials":["K"]},{"fullName":["Kanagawa S"],"firstName":["Shuzo"],"lastName":["Kanagawa"],"initials":["S"]},{"fullName":["Ohmagari N"],"firstName":["Norio"],"lastName":["Ohmagari"],"initials":["N"]}]}],"journalInfo":[{"issue":["9"],"volume":["22"],"journalIssueId":["2409892"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1683-1685"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Phylogeography","Chikungunya virus","Japan","Dengue","Conjunctivitis","Travel","Cuba","Viral Infections","Global Health","Chikungunya Fever","Zika Virus","Imported Viral Diseases"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994370?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994370"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2209.160603"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-18"],"dateOfRevision":["2016-09-08"],"firstPublicationDate":["2016-09-01"],"cprojectID":"PMC4994370"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8pJ9hGurOzZIcM","_score":1,"_source":{"id":["27583097"],"source":["MED"],"pmid":["27583097"],"pmcid":["PMC4992390"],"doi":["10.11604/pamj.2016.24.33.9749"],"title":["Zika: what we know and don't know."],"authorString":["Diop D, Rambe DS, Sanicas M."],"authorList":[{"author":[{"fullName":["Diop D"],"firstName":["Doudou"],"lastName":["Diop"],"initials":["D"],"affiliation":["EPLS Biomedical Research Center, Saint-Louis, Senegal."]},{"fullName":["Rambe DS"],"firstName":["Dirga Sakti"],"lastName":["Rambe"],"initials":["DS"],"affiliation":["Faculty of Medicine of University of Indonesia, Jakarta, Indonesia."]},{"fullName":["Sanicas M"],"firstName":["Melvin"],"lastName":["Sanicas"],"initials":["M"],"affiliation":["Bill and Melinda Gates Foundation, Seattle, WA, USA."]}]}],"journalInfo":[{"volume":["24"],"journalIssueId":["2425787"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["The Pan African medical journal"],"ISOAbbreviation":["Pan Afr Med J"],"medlineAbbreviation":["Pan Afr Med J"],"NLMid":["101517926"],"ESSN":["1937-8688"]}]}],"pubYear":["2016"],"pageInfo":["33"],"abstractText":["Since the initial reports of a link between Zika and microcephaly, researchers across the world began working toward understanding the virus. In a short amount of time, Zika has become a household name, prompting worldwide concern. The virus is spread rapidly by mosquito bites. We currently do not have a vaccine for Zika. But with the recent findings, vaccine companies are mobilizing their resources to expedite efforts to shave years off the typical decade-long process of vaccine development."],"affiliation":["EPLS Biomedical Research Center, Saint-Louis, Senegal."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Virus","Disease","Prevention","Treatment","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4992390?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4992390"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.11604/pamj.2016.24.33.9749"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-01"],"dateOfRevision":["2016-09-04"],"electronicPublicationDate":["2016-05-09"],"firstPublicationDate":["2016-05-09"],"cprojectID":"PMC4992390"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8r_8VqgcuJwvkI","_score":1,"_source":{"id":["27583132"],"source":["MED"],"pmid":["27583132"],"pmcid":["PMC4972086"],"doi":["10.12688/f1000research.9110.1"],"title":["An open RNA-Seq data analysis pipeline tutorial with an example of reprocessing data from a recent Zika virus study."],"authorString":["Wang Z, Ma'ayan A."],"authorList":[{"author":[{"fullName":["Wang Z"],"firstName":["Zichen"],"lastName":["Wang"],"initials":["Z"],"affiliation":["Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; BD2K-LINCS Data Coordination and Integration Center, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; Mount Sinai Knowledge Management Center for Illuminating the Druggable Genome, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA."]},{"fullName":["Ma'ayan A"],"firstName":["Avi"],"lastName":["Ma'ayan"],"initials":["A"],"affiliation":["Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; BD2K-LINCS Data Coordination and Integration Center, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; Mount Sinai Knowledge Management Center for Illuminating the Druggable Genome, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1415-1286","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["1574"],"abstractText":["RNA-seq analysis is becoming a standard method for global gene expression profiling. However, open and standard pipelines to perform RNA-seq analysis by non-experts remain challenging due to the large size of the raw data files and the hardware requirements for running the alignment step. Here we introduce a reproducible open source RNA-seq pipeline delivered as an IPython notebook and a Docker image. The pipeline uses state-of-the-art tools and can run on various platforms with minimal configuration overhead. The pipeline enables the extraction of knowledge from typical RNA-seq studies by generating interactive principal component analysis (PCA) and hierarchical clustering (HC) plots, performing enrichment analyses against over 90 gene set libraries, and obtaining lists of small molecules that are predicted to either mimic or reverse the observed changes in mRNA expression. We apply the pipeline to a recently published RNA-seq dataset collected from human neuronal progenitors infected with the Zika virus (ZIKV). In addition to confirming the presence of cell cycle genes among the genes that are downregulated by ZIKV, our analysis uncovers significant overlap with upregulated genes that when knocked out in mice induce defects in brain morphology. This result potentially points to the molecular processes associated with the microcephaly phenotype observed in newborns from pregnant mothers infected with the virus. In addition, our analysis predicts small molecules that can either mimic or reverse the expression changes induced by ZIKV. The IPython notebook and Docker image are freely available at: http://nbviewer.jupyter.org/github/maayanlab/Zika-RNAseq-Pipeline/blob/master/Zika.ipynb and https://hub.docker.com/r/maayanlab/zika/."],"affiliation":["Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; BD2K-LINCS Data Coordination and Integration Center, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA; Mount Sinai Knowledge Management Center for Illuminating the Druggable Genome, Icahn School of Medicine at Mount Sinai, New York, NY, Box 1603, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Gene expression analysis","systems biology","Rna-seq","Bioinformatics Pipeline"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972086?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972086"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.9110.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-09-01"],"dateOfCreation":["2016-09-01"],"dateOfRevision":["2016-09-04"],"electronicPublicationDate":["2016-07-05"],"firstPublicationDate":["2016-07-05"],"cprojectID":"PMC4972086"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr9KJ9hGurOzZIcP","_score":1,"_source":{"id":["27554037"],"source":["MED"],"pmid":["27554037"],"pmcid":["PMC4995374"],"doi":["10.1038/srep32227"],"title":["An ultrasensitive electrogenerated chemiluminescence-based immunoassay for specific detection of Zika virus."],"authorString":["Acharya D, Bastola P, Le L, Paul AM, Fernandez E, Diamond MS, Miao W, Bai F."],"authorList":[{"author":[{"fullName":["Acharya D"],"firstName":["Dhiraj"],"lastName":["Acharya"],"initials":["D"],"affiliation":["Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]},{"fullName":["Bastola P"],"firstName":["Pradip"],"lastName":["Bastola"],"initials":["P"],"affiliation":["Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]},{"fullName":["Le L"],"firstName":["Linda"],"lastName":["Le"],"initials":["L"],"affiliation":["Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]},{"fullName":["Paul AM"],"firstName":["Amber M"],"lastName":["Paul"],"initials":["AM"],"affiliation":["Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]},{"fullName":["Fernandez E"],"firstName":["Estefania"],"lastName":["Fernandez"],"initials":["E"],"affiliation":["Departments of Medicine, Molecular Microbiology, Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA."]},{"fullName":["Diamond MS"],"firstName":["Michael S"],"lastName":["Diamond"],"initials":["MS"],"affiliation":["Departments of Medicine, Molecular Microbiology, Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA."]},{"fullName":["Miao W"],"firstName":["Wujian"],"lastName":["Miao"],"initials":["W"],"affiliation":["Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]},{"fullName":["Bai F"],"firstName":["Fengwei"],"lastName":["Bai"],"initials":["F"],"affiliation":["Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["32227"],"abstractText":["Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research."],"affiliation":["Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4995374?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4995374"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep32227"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-24"],"dateOfRevision":["2016-08-31"],"electronicPublicationDate":["2016-08-24"],"firstPublicationDate":["2016-08-24"],"cprojectID":"PMC4995374"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsAfJ9hGurOzZIcT","_score":1,"_source":{"id":["27555311"],"source":["MED"],"pmid":["27555311"],"pmcid":["PMC4999549"],"doi":["10.1128/mbio.01114-16"],"title":["A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 Epidemic in Brazil as a Genetic Platform for Studies of Virus-Host Interactions and Vaccine Development."],"authorString":["Tsetsarkin KA, Kenney H, Chen R, Liu G, Manukyan H, Whitehead SS, Laassri M, Chumakov K, Pletnev AG."],"authorList":[{"author":[{"fullName":["Tsetsarkin KA"],"firstName":["Konstantin A"],"lastName":["Tsetsarkin"],"initials":["KA"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA."]},{"fullName":["Kenney H"],"firstName":["Heather"],"lastName":["Kenney"],"initials":["H"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA."]},{"fullName":["Chen R"],"firstName":["Rubing"],"lastName":["Chen"],"initials":["R"],"affiliation":["Department of Pathology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA."]},{"fullName":["Liu G"],"firstName":["Guangping"],"lastName":["Liu"],"initials":["G"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA."]},{"fullName":["Manukyan H"],"firstName":["Hasmik"],"lastName":["Manukyan"],"initials":["H"],"affiliation":["Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA."]},{"fullName":["Whitehead SS"],"firstName":["Stephen S"],"lastName":["Whitehead"],"initials":["SS"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA."]},{"fullName":["Laassri M"],"firstName":["Majid"],"lastName":["Laassri"],"initials":["M"],"affiliation":["Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA."]},{"fullName":["Chumakov K"],"firstName":["Konstantin"],"lastName":["Chumakov"],"initials":["K"],"authorId":[{"_":"0000-0003-3002-6247","$":{"type":"ORCID"}}],"affiliation":["Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, Maryland, USA."]},{"fullName":["Pletnev AG"],"firstName":["Alexander G"],"lastName":["Pletnev"],"initials":["AG"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA apletnev@niaid.nih.gov."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-3002-6247","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["7"],"journalIssueId":["2426379"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"abstractText":["An arthropod-borne virus, Zika virus (ZIKV), has recently emerged as a major human pathogen. Associated with complications during perinatal development and Guillain-Barré syndrome in adults, ZIKV raises new challenges for understanding the molecular determinants of flavivirus pathogenesis. This underscores the necessity for the development of a reverse genetic system based on an epidemic ZIKV strain. Here, we describe the generation and characterization in cell cultures of an infectious cDNA clone of ZIKV isolated from the 2015 epidemic in Brazil. The cDNA-derived ZIKV replicated efficiently in a variety of cell lines, including those of both neuronal and placental origin. We observed that the growth of cDNA-derived virus was attenuated compared to the growth of the parental isolate in most cell lines, which correlates with substantial differences in sequence heterogeneity between these viruses that were determined by deep-sequencing analysis. Our findings support the role of genetic diversity in maintaining the replicative fitness of viral populations under changing conditions. Moreover, these results indicate that caution should be exercised when interpreting the results of reverse-genetics experiments in attempts to accurately predict the biology of natural viruses. Finally, a Vero cell-adapted cDNA clone of ZIKV was generated that can be used as a convenient platform for studies aimed at the development of ZIKV vaccines and therapeutics.The availability of genetic tools and laboratory models determines the progress in understanding mechanisms of virus emergence and pathogenesis. Recent large-scale outbreaks of Zika virus (ZIKV) that were linked to complications during perinatal development and Guillain-Barré syndrome in adults emphasize the urgency for the development of a reverse-genetics system based on an epidemic ZIKV strain. Here, we report a stable infectious cDNA clone for ZIKV isolated during the 2015 epidemic in Brazil, as well as a Vero cell-adapted version of it, which will be used for virus-host interaction studies and vaccine development."],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999549?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999549"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.01114-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-24"],"dateOfRevision":["2016-08-28"],"electronicPublicationDate":["2016-08-23"],"firstPublicationDate":["2016-08-23"],"cprojectID":"PMC4999549"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsBghOUS2orL1ISi","_score":1,"_source":{"id":["27560129"],"source":["MED"],"pmid":["27560129"],"pmcid":["PMC4999274"],"doi":["10.1371/journal.pntd.0004877"],"title":["Zika Fetal Neuropathogenesis: Etiology of a Viral Syndrome."],"authorString":["Klase ZA, Khakhina S, Schneider Ade B, Callahan MV, Glasspool-Malone J, Malone R."],"authorList":[{"author":[{"fullName":["Klase ZA"],"firstName":["Zachary A"],"lastName":["Klase"],"initials":["ZA"],"affiliation":["Department of Biological Sciences, University of the Sciences, Philadelphia, Pennsylvania, United States of America."]},{"fullName":["Khakhina S"],"firstName":["Svetlana"],"lastName":["Khakhina"],"initials":["S"],"affiliation":["Department of Biological Sciences, University of the Sciences, Philadelphia, Pennsylvania, United States of America."]},{"fullName":["Schneider Ade B"],"firstName":["Adriano De Bernardi"],"lastName":["Schneider"],"initials":["Ade B"],"affiliation":["Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina, United States of America."]},{"fullName":["Callahan MV"],"firstName":["Michael V"],"lastName":["Callahan"],"initials":["MV"],"affiliation":["Zika Foundation, College Station, Texas, United States of America."]},{"fullName":["Glasspool-Malone J"],"firstName":["Jill"],"lastName":["Glasspool-Malone"],"initials":["J"],"affiliation":["Global Clinical Scholars Research Training Program, Harvard Medical School, Boston, Massachusetts, United States of America."]},{"fullName":["Malone R"],"firstName":["Robert"],"lastName":["Malone"],"initials":["R"],"authorId":[{"_":"0000-0003-0340-7490","$":{"type":"ORCID"}}],"affiliation":["Global Clinical Scholars Research Training Program, Harvard Medical School, Boston, Massachusetts, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-0340-7490","$":{"type":"ORCID"}},{"_":"0000-0003-0340-7490","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004877"],"abstractText":["The ongoing Zika virus epidemic in the Americas and the observed association with both fetal abnormalities (primary microcephaly) and adult autoimmune pathology (Guillain-Barré syndrome) has brought attention to this neglected pathogen. While initial case studies generated significant interest in the Zika virus outbreak, larger prospective epidemiology and basic virology studies examining the mechanisms of Zika viral infection and associated pathophysiology are only now starting to be published. In this review, we analyze Zika fetal neuropathogenesis from a comparative pathology perspective, using the historic metaphor of \"TORCH\" viral pathogenesis to provide context. By drawing parallels to other viral infections of the fetus, we identify common themes and mechanisms that may illuminate the observed pathology. The existing data on the susceptibility of various cells to both Zika and other flavivirus infections are summarized. Finally, we highlight relevant aspects of the known molecular mechanisms of flavivirus replication."],"affiliation":["Department of Biological Sciences, University of the Sciences, Philadelphia, Pennsylvania, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999274?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999274"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004877"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-26"],"electronicPublicationDate":["2016-08-25"],"firstPublicationDate":["2016-08-25"],"cprojectID":"PMC4999274"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr_u_8VqgcuJwvkR","_score":1,"_source":{"id":["27428993"],"source":["MED"],"pmid":["27428993"],"pmcid":["PMC4974529"],"doi":["10.3390/v8070194"],"title":["RNA Encapsidation and Packaging in the Phleboviruses."],"authorString":["Hornak KE, Lanchy JM, Lodmell JS."],"authorList":[{"author":[{"fullName":["Hornak KE"],"firstName":["Katherine E"],"lastName":["Hornak"],"initials":["KE"],"affiliation":["Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA. katherine.hornak@umontana.edu."]},{"fullName":["Lanchy JM"],"firstName":["Jean-Marc"],"lastName":["Lanchy"],"initials":["JM"],"affiliation":["Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA. jean-marc.lanchy@umontana.edu."]},{"fullName":["Lodmell JS"],"firstName":["J Stephen"],"lastName":["Lodmell"],"initials":["JS"],"affiliation":["Center for Biomolecular Structure and Dynamics, University of Montana, Missoula, MT 59812, USA. stephen.lodmell@umontana.edu."]}]}],"journalInfo":[{"issue":["7"],"volume":["8"],"journalIssueId":["2423133"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2016"],"abstractText":["The Bunyaviridae represents the largest family of segmented RNA viruses, which infect a staggering diversity of plants, animals, and insects. Within the family Bunyaviridae, the Phlebovirus genus includes several important human and animal pathogens, including Rift Valley fever virus (RVFV), severe fever with thrombocytopenia syndrome virus (SFTSV), Uukuniemi virus (UUKV), and the sandfly fever viruses. The phleboviruses have small tripartite RNA genomes that encode a repertoire of 5-7 proteins. These few proteins accomplish the daunting task of recognizing and specifically packaging a tri-segment complement of viral genomic RNA in the midst of an abundance of host components. The critical nucleation events that eventually lead to virion production begin early on in the host cytoplasm as the first strands of nascent viral RNA (vRNA) are synthesized. The interaction between the vRNA and the viral nucleocapsid (N) protein effectively protects and masks the RNA from the host, and also forms the ribonucleoprotein (RNP) architecture that mediates downstream interactions and drives virion formation. Although the mechanism by which all three genomic counterparts are selectively co-packaged is not completely understood, we are beginning to understand the hierarchy of interactions that begins with N-RNA packaging and culminates in RNP packaging into new virus particles. In this review we focus on recent progress that highlights the molecular basis of RNA genome packaging in the phleboviruses."],"affiliation":["Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA. katherine.hornak@umontana.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Phlebovirus","Bunyavirus","Rna Packaging"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974529?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974529"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v8070194"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"cprojectID":"PMC4974529"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsBy_8VqgcuJwvkb","_score":1,"_source":{"id":["27273325"],"source":["MED"],"pmid":["27273325"],"pmcid":["PMC4981682"],"doi":["10.1038/hdy.2016.35"],"title":["Population genetics of the Asian tiger mosquito Aedes albopictus, an invasive vector of human diseases."],"authorString":["Goubert C, Minard G, Vieira C, Boulesteix M."],"authorList":[{"author":[{"fullName":["Goubert C"],"firstName":["C"],"lastName":["Goubert"],"initials":["C"],"affiliation":["Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Villeurbanne, France."]},{"fullName":["Minard G"],"firstName":["G"],"lastName":["Minard"],"initials":["G"],"affiliation":["Department of Biosciences, Metapopulation Research Center, University of Helsinki, Helsinki, Finland."]},{"fullName":["Vieira C"],"firstName":["C"],"lastName":["Vieira"],"initials":["C"],"affiliation":["Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Villeurbanne, France."]},{"fullName":["Boulesteix M"],"firstName":["M"],"lastName":["Boulesteix"],"initials":["M"],"affiliation":["Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Villeurbanne, France."]}]}],"journalInfo":[{"issue":["3"],"volume":["117"],"journalIssueId":["2440265"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Heredity"],"ISOAbbreviation":["Heredity (Edinb)"],"medlineAbbreviation":["Heredity (Edinb)"],"NLMid":["0373007"],"ISSN":["0018-067X"],"ESSN":["1365-2540"]}]}],"pubYear":["2016"],"pageInfo":["125-134"],"abstractText":["The Asian tiger mosquito Aedes albopictus is currently one of the most threatening invasive species in the world. Native to Southeast Asia, the species has spread throughout the world in the past 30 years and is now present in every continent but Antarctica. Because it was the main vector of recent Dengue and Chikungunya outbreaks, and because of its competency for numerous other viruses and pathogens such as the Zika virus, A. albopictus stands out as a model species for invasive diseases vector studies. A synthesis of the current knowledge about the genetic diversity of A. albopictus is needed, knowing the interplays between the vector, the pathogens, the environment and their epidemiological consequences. Such resources are also valuable for assessing the role of genetic diversity in the invasive success. We review here the large but sometimes dispersed literature about the population genetics of A. albopictus. We first debate about the experimental design of these studies and present an up-to-date assessment of the available molecular markers. We then summarize the main genetic characteristics of natural populations and synthesize the available data regarding the worldwide structuring of the vector. Finally, we pinpoint the gaps that remain to be addressed and suggest possible research directions."],"affiliation":["Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR5558, Villeurbanne, France."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981682?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981682"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/hdy.2016.35"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-10"],"dateOfRevision":["2016-09-01"],"electronicPublicationDate":["2016-06-08"],"firstPublicationDate":["2016-06-08"],"cprojectID":"PMC4981682"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsCy_8VqgcuJwvke","_score":1,"_source":{"id":["27519588"],"source":["MED"],"pmid":["27519588"],"pmcid":["PMC4983072"],"doi":["10.1186/s13071-016-1734-8"],"title":["The importance of male body size on sperm uptake and usage, and female fecundity in Aedes aegypti and Aedes albopictus."],"authorString":["De Jesus CE, Reiskind MH."],"authorList":[{"author":[{"fullName":["De Jesus CE"],"firstName":["Carrie E"],"lastName":["De Jesus"],"initials":["CE"],"affiliation":["Present address: Delta Vector Control District, P.O. Box 310, Visalia, CA, 93279-0310, USA."]},{"fullName":["Reiskind MH"],"firstName":["Michael H"],"lastName":["Reiskind"],"initials":["MH"],"affiliation":["Department of Entomology, North Carolina State University, Box 7613, Raleigh, NC, 27695, USA. mhreiski@ncsu.edu."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["447"],"abstractText":["Adult mosquito density is a critical factor in the transmission of arboviruses by container Aedes spp. mosquitoes. Female fecundity drives population growth, and therefore contributes to adult mosquito density. Previous studies have focused on female body size as the major determinant of fecundity, paying little attention to male condition. In this study, we examined the effects of male body size on the abundance of sperm in spermatheca, depletion of sperm over time, and female fecundity.We generated males in two size classes using different larval densities, and allowed them to mate with females generated from a moderately dense larval environment. We counted sperm in female spermatheca in a sample of females immediately after mating, then every week for four weeks post-mating. We provided weekly blood meals to females and determined their fecundity over four weeks after the initial blood meal.We found significantly more sperm in Aedes albopictus females than in Aedes aegypti, and detected depletion of sperm in Ae. aegypti, but not in Ae. albopictus. We did not see significant differences in number of sperm in spermathecae in relation to male body size in either species over subsequent gonotrophic cycles. We found a significant effect of male body size on fecundity in Ae. albopictus, but not Ae. aegypti, with a 46 % increase in fecundity for female Ae. albopictus offered four blood meals.Our results suggest substantial differences in the mating biology of these ecologically similar species and the importance of considering males in understanding female fecundity. The substantial increase in fecundity in Ae. albopictus has implications for population growth, estimating vector density, and modeling the transmission of pathogens."],"affiliation":["Present address: Delta Vector Control District, P.O. Box 310, Visalia, CA, 93279-0310, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Mating","Seminal Fluid Proteins","Male Fitness","Accessory Gland Proteins","Container Mosquitoes"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4983072?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4983072"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1734-8"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-13"],"dateOfRevision":["2016-08-18"],"electronicPublicationDate":["2016-08-12"],"firstPublicationDate":["2016-08-12"],"cprojectID":"PMC4983072"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsErhOUS2orL1ISs","_score":1,"_source":{"id":["27364784"],"source":["MED"],"pmid":["27364784"],"pmcid":["PMC5006602"],"doi":["10.1016/j.ebiom.2016.06.026"],"title":["Infectivity of Immature Neurons to Zika Virus: A Link to Congenital Zika Syndrome."],"authorString":["Hughes BW, Addanki KC, Sriskanda AN, McLean E, Bagasra O."],"authorList":[{"author":[{"fullName":["Hughes BW"],"firstName":["Brandon W"],"lastName":["Hughes"],"initials":["BW"],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States."]},{"fullName":["Addanki KC"],"firstName":["Krishna C"],"lastName":["Addanki"],"initials":["KC"],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States."]},{"fullName":["Sriskanda AN"],"firstName":["Ahila N"],"lastName":["Sriskanda"],"initials":["AN"],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States."]},{"fullName":["McLean E"],"firstName":["Ewen"],"lastName":["McLean"],"initials":["E"],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States."]},{"fullName":["Bagasra O"],"firstName":["Omar"],"lastName":["Bagasra"],"initials":["O"],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States. Electronic address: obagasra@claflin.edu."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2444983"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["65-70"],"abstractText":["Epidemiological data strongly suggest that microcephaly cases in Brazil are associated with the ongoing epidemic of Zika virus (ZIKV). In order to further solidify the possible link, we investigated the infectivity of ZIKV using various neuroblastoma (NB) cell lines.Six undifferentiated, two terminally differentiated and two retinoic acid (RA) -induced, partially differentiated cell lines were exposed to ZIKV strain PRVABC59, which is genetically similar to the French Polynesia strain, with 97-100% genetic homology to the current ZIKV strain found in Brazil. All infections were confirmed by real-time PCR (RT-qPCR), immunofluorescence assay (IFA) probing with anti-flavivirus E antibody, and evaluation of cytopathic effects.ZIKV infected all six undifferentiated NB cell lines. In five out of six NB cell lines, between 90 and 70% cells were positive by IFA whereas for one cell line, CCL-127, ~80% of cells were positive for ZIKV as determined by IFA but showed persistent infection. Two differentiated cell lines, JFEN and T-268, were highly resistant to ZIKV with <1% of the cells being susceptible, as determined by IFA and confirmed by qRT-PCR. Two retinoic acid (RA)-induced NB partially differentiated cell lines showed no difference in permissiveness as compared to their undifferentiated mother cell lines.These findings strengthen the reported association between high incidences of microcephaly and ZIKV infection in newborns in Brazil. Our results suggest that the undifferentiated neurons are highly permissive to ZIKV infection, as one would expect during the early stages of neurogenesis in fetal brains; whereas differentiated neurons, representative of adult brain neurons, are relatively resistant to the virus, which explains the rare occurrence of neurological complications in adults infected with ZIKV. Our studies confirm the neurotropism of the ZIKV strain closely related to the current epidemic in Latin America."],"affiliation":["Claflin University, South Carolina Center for Biotechnology, 400 Magnolia Street, 29115 Orangeburg, SC, United States."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Neurogenesis","Neuroblastoma","Microcephaly","Undifferentiated","Terminally Differentiated","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006602?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006602"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.06.026"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-02"],"electronicPublicationDate":["2016-06-23"],"firstPublicationDate":["2016-06-23"],"cprojectID":"PMC5006602"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsMd_8VqgcuJwvk2","_score":1,"_source":{"id":["27475896"],"source":["MED"],"pmid":["27475896"],"pmcid":["PMC5006724"],"doi":["10.1016/j.ebiom.2016.07.026"],"title":["Finding Clues for Congenital Zika Syndrome: Zika Virus Selective Infection of Immature Neurons."],"authorString":["Maestre AM, Fernández-Sesma A."],"authorList":[{"author":[{"fullName":["Maestre AM"],"firstName":["Ana M"],"lastName":["Maestre"],"initials":["AM"],"affiliation":["Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, United States."]},{"fullName":["Fernández-Sesma A"],"firstName":["Ana"],"lastName":["Fernández-Sesma"],"initials":["A"],"affiliation":["Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, United States; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, United States. Electronic address: ana.sesma@mssm.edu."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2444983"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["7-8"],"affiliation":["Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, United States."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006724?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006724"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.07.026"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-02"],"electronicPublicationDate":["2016-07-25"],"firstPublicationDate":["2016-07-25"],"cprojectID":"PMC5006724"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsKC_8VqgcuJwvkz","_score":1,"_source":{"id":["27578761"],"source":["MED"],"pmid":["27578761"],"pmcid":["PMC4999558"],"doi":["10.1128/mbio.01381-16"],"title":["Moonshot Science-Risks and Benefits."],"authorString":["Casadevall A, Fang FC."],"authorList":[{"author":[{"fullName":["Casadevall A"],"firstName":["Arturo"],"lastName":["Casadevall"],"initials":["A"],"authorId":[{"_":"0000-0002-9402-9167","$":{"type":"ORCID"}}],"affiliation":["Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA acasade1@jhu.edu."]},{"fullName":["Fang FC"],"firstName":["Ferric C"],"lastName":["Fang"],"initials":["FC"],"authorId":[{"_":"0000-0002-3243-110X","$":{"type":"ORCID"}}],"affiliation":["Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle, Washington, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9402-9167","$":{"type":"ORCID"}},{"_":"0000-0002-3243-110X","$":{"type":"ORCID"}},{"_":"0000-0002-3243-110X","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["7"],"journalIssueId":["2426379"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"abstractText":["Ever since the successful Apollo 11 Moon landing in 1969, a \"moonshot\" has come to signify a bold effort to achieve a seemingly impossible task. The Obama administration recently called for a moonshot to cure cancer, an initiative that has elicited mixed responses from researchers who welcome additional funding but worry about raising expectations. We suggest that a successful moonshot requires a sufficient understanding of the basic science underlying a problem in question so that efforts can be focused on engineering a solution. Current gaps in our basic knowledge of cancer biology make the cancer moonshot a uniquely challenging endeavor. Nevertheless, history has shown that intensive research efforts have frequently yielded conceptual and technological breakthroughs with unanticipated benefits for society. We expect that this effort will be no different."],"affiliation":["Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA acasade1@jhu.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999558?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4999558"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.01381-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-31"],"dateOfRevision":["2016-09-02"],"electronicPublicationDate":["2016-08-30"],"firstPublicationDate":["2016-08-30"],"cprojectID":"PMC4999558"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsZJ9hGurOzZIYZ","_score":1,"_source":{"id":["26101272"],"source":["MED"],"pmid":["26101272"],"pmcid":["PMC4530765"],"doi":["10.4269/ajtmh.15-0022"],"title":["Detection of Zika Virus Infection in Thailand, 2012-2014."],"authorString":["Buathong R, Hermann L, Thaisomboonsuk B, Rutvisuttinunt W, Klungthong C, Chinnawirotpisan P, Manasatienkij W, Nisalak A, Fernandez S, Yoon IK, Akrasewi P, Plipat T."],"authorList":[{"author":[{"fullName":["Buathong R"],"firstName":["Rome"],"lastName":["Buathong"],"initials":["R"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Hermann L"],"firstName":["Laura"],"lastName":["Hermann"],"initials":["L"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada laura.hermann@afrims.org."]},{"fullName":["Thaisomboonsuk B"],"firstName":["Butsaya"],"lastName":["Thaisomboonsuk"],"initials":["B"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Rutvisuttinunt W"],"firstName":["Wiriya"],"lastName":["Rutvisuttinunt"],"initials":["W"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Klungthong C"],"firstName":["Chonticha"],"lastName":["Klungthong"],"initials":["C"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Chinnawirotpisan P"],"firstName":["Piyawan"],"lastName":["Chinnawirotpisan"],"initials":["P"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Manasatienkij W"],"firstName":["Wudtichai"],"lastName":["Manasatienkij"],"initials":["W"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Nisalak A"],"firstName":["Ananda"],"lastName":["Nisalak"],"initials":["A"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Fernandez S"],"firstName":["Stefan"],"lastName":["Fernandez"],"initials":["S"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Yoon IK"],"firstName":["In-Kyu"],"lastName":["Yoon"],"initials":["IK"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Akrasewi P"],"firstName":["Passakorn"],"lastName":["Akrasewi"],"initials":["P"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]},{"fullName":["Plipat T"],"firstName":["Tanarak"],"lastName":["Plipat"],"initials":["T"],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."]}]}],"journalInfo":[{"issue":["2"],"volume":["93"],"journalIssueId":["2311825"],"dateOfPublication":["2015 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2015"],"printPublicationDate":["2015-08-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. 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These endemic cases, combined with previous reports in travelers, provide evidence that ZIKV is widespread throughout Thailand."],"affiliation":["Department of Disease Control, Bureau of Epidemiology, Ministry of Public Health, Nonthaburi, Thailand; Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Department of Medicine, University of Toronto, Toronto, Ontario, Canada."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Case Reports","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"agency":["Canadian Institutes of Health Research"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Child"]},{"majorTopic_YN":["N"],"descriptorName":["Thailand"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4530765?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4530765"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.15-0022"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["9"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-10-27"],"dateOfCreation":["2015-08-07"],"dateOfRevision":["2016-02-03"],"electronicPublicationDate":["2015-06-22"],"firstPublicationDate":["2015-06-22"],"cprojectID":"PMC4530765"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSX_8VqgcuJwvfy","_score":1,"_source":{"id":["22460397"],"source":["MED"],"pmid":["22460397"],"pmcid":["PMC3167654"],"doi":["10.3134/ehtj.10.163"],"title":["Emerging viral threats in Gabon: health capacities and response to the risk of emerging zoonotic diseases in Central Africa."],"authorString":["Bourgarel M, Wauquier N, Gonzalez JP."],"authorList":[{"author":[{"fullName":["Bourgarel M"],"firstName":["M"],"lastName":["Bourgarel"],"initials":["M"]},{"fullName":["Wauquier N"],"firstName":["N"],"lastName":["Wauquier"],"initials":["N"]},{"fullName":["Gonzalez JP"],"firstName":["J-P"],"lastName":["Gonzalez"],"initials":["JP"]}]}],"journalInfo":[{"volume":["3"],"journalIssueId":["1959578"],"dateOfPublication":["2010 "],"monthOfPublication":["0"],"yearOfPublication":["2010"],"printPublicationDate":["2010-01-01"],"journal":[{"title":["Emerging health threats journal"],"ISOAbbreviation":["Emerg Health Threats J"],"medlineAbbreviation":["Emerg Health Threats J"],"NLMid":["101484599"],"ESSN":["1752-8550"]}]}],"pubYear":["2010"],"pageInfo":["e7"],"abstractText":["Emerging infectious diseases (EID) are currently the major threat to public health worldwide and most EID events have involved zoonotic infectious agents. Central Africa in general and Gabon in particular are privileged areas for the emergence of zoonotic EIDs. Indeed, human incursions in Gabonese forests for exploitation purposes lead to intensified contacts between humans and wildlife thus generating an increased risk of emergence of zoonotic diseases. In Gabon, 51 endemic or potential endemic viral infectious diseases have been reported. Among them, 22 are of zoonotic origin and involve 12 families of viruses. The most notorious are dengue, yellow fever, ebola, marburg, Rift Valley fever and chikungunya viruses. Potential EID due to wildlife in Gabon are thereby plentiful and need to be inventoried. The Gabonese Public Health system covers geographically most of the country allowing a good access to sanitary information and efficient monitoring of emerging diseases. However, access to treatment and prevention is better in urban areas where medical structures are more developed and financial means are concentrated even though the population is equally distributed between urban and rural areas. In spite of this, Gabon could be a good field for investigating the emergence or re-emergence of zoonotic EID. Indeed Gabonese health research structures such as CIRMF, advantageously located, offer high quality researchers and facilities that study pathogens and wildlife ecology, aiming toward a better understanding of the contact and transmission mechanisms of new pathogens from wildlife to human, the emergence of zoonotic EID and the breaking of species barriers by pathogens."],"affiliation":["Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UPR AGIRs, Campus International de Baillarguet, Montpellier cedex 5, France."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3167654?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3167654"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22460397"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22460397&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3134/ehtj.10.163"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2012-08-23"],"dateOfCreation":["2012-03-30"],"electronicPublicationDate":["2010-06-03"],"firstPublicationDate":["2010-06-03"],"cprojectID":"PMC3167654"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsH_8VqgcuJwvgb","_score":1,"_source":{"id":["25606971"],"source":["MED"],"pmid":["25606971"],"pmcid":["PMC4306835"],"doi":["10.3390/v7010219"],"title":["Usutu virus: an emerging flavivirus in Europe."],"authorString":["Ashraf U, Ye J, Ruan X, Wan S, Zhu B, Cao S."],"authorList":[{"author":[{"fullName":["Ashraf U"],"firstName":["Usama"],"lastName":["Ashraf"],"initials":["U"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. usamaashraf1523@gmail.com."]},{"fullName":["Ye J"],"firstName":["Jing"],"lastName":["Ye"],"initials":["J"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. yeah_zaizai@hotmail.com."]},{"fullName":["Ruan X"],"firstName":["Xindi"],"lastName":["Ruan"],"initials":["X"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. 258390599@qq.com."]},{"fullName":["Wan S"],"firstName":["Shengfeng"],"lastName":["Wan"],"initials":["S"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. wansf126@126.com."]},{"fullName":["Zhu B"],"firstName":["Bibo"],"lastName":["Zhu"],"initials":["B"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. zhubibohzau@126.com."]},{"fullName":["Cao S"],"firstName":["Shengbo"],"lastName":["Cao"],"initials":["S"],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. sbcao@mail.hzau.edu.cn."]}]}],"journalInfo":[{"issue":["1"],"volume":["7"],"journalIssueId":["2249728"],"dateOfPublication":["2015 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2015"],"pageInfo":["219-238"],"abstractText":["Usutu virus (USUV) is an African mosquito-borne flavivirus belonging to the Japanese encephalitis virus serocomplex. USUV is closely related to Murray Valley encephalitis virus, Japanese encephalitis virus, and West Nile virus. USUV was discovered in South Africa in 1959. In Europe, the first true demonstration of circulation of USUV was reported in Austria in 2001 with a significant die-off of Eurasian blackbirds. In the subsequent years, USUV expanded to neighboring countries, including Italy, Germany, Spain, Hungary, Switzerland, Poland, England, Czech Republic, Greece, and Belgium, where it caused unusual mortality in birds. In 2009, the first two human cases of USUV infection in Europe have been reported in Italy, causing meningoencephalitis in immunocompromised patients. This review describes USUV in terms of its life cycle, USUV surveillance from Africa to Europe, human cases, its cellular tropism and pathogenesis, its genetic relationship with other flaviviruses, genetic diversity among USUV strains, its diagnosis, and a discussion of the potential future threat to Asian countries."],"affiliation":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. usamaashraf1523@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis Viruses, Japanese"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis, Arbovirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Europe"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4306835?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4306835"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v7010219"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-08-31"],"dateOfCreation":["2015-01-22"],"dateOfRevision":["2015-02-19"],"electronicPublicationDate":["2015-01-19"],"firstPublicationDate":["2015-01-19"],"cprojectID":"PMC4306835"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSY_8VqgcuJwvfz","_score":1,"_source":{"id":["23936579"],"source":["MED"],"pmid":["23936579"],"pmcid":["PMC3731215"],"doi":["10.1371/journal.pntd.0002348"],"title":["Aedes (Stegomyia) albopictus (Skuse): a potential vector of Zika virus in Singapore."],"authorString":["Wong PS, Li MZ, Chong CS, Ng LC, Tan CH."],"authorList":[{"author":[{"fullName":["Wong PS"],"firstName":["Pei-Sze Jeslyn"],"lastName":["Wong"],"initials":["PS"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Li MZ"],"firstName":["Mei-zhi Irene"],"lastName":["Li"],"initials":["MZ"]},{"fullName":["Chong CS"],"firstName":["Chee-Seng"],"lastName":["Chong"],"initials":["CS"]},{"fullName":["Ng LC"],"firstName":["Lee-Ching"],"lastName":["Ng"],"initials":["LC"]},{"fullName":["Tan CH"],"firstName":["Cheong-Huat"],"lastName":["Tan"],"initials":["CH"]}]}],"journalInfo":[{"issue":["8"],"volume":["7"],"journalIssueId":["2070282"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2013"],"pageInfo":["e2348"],"abstractText":["Zika virus (ZIKV) is a little known arbovirus until it caused a major outbreak in the Pacific Island of Yap in 2007. Although the virus has a wide geographic distribution, most of the known vectors are sylvatic Aedes mosquitoes from Africa where the virus was first isolated. Presently, Ae. aegypti is the only known vector to transmit the virus outside the African continent, though Ae. albopictus has long been a suspected vector. Currently, Ae. albopictus has been shown capable of transmitting more than 20 arboviruses and its notoriety as an important vector came to light during the recent chikungunya pandemic. The vulnerability of Singapore to emerging infectious arboviruses has stimulated our interest to determine the competence of local Ae. albopictus to transmit ZIKV.To determine the competence of Ae. albopictus to ZIKV, we orally infected local mosquito strains to a Ugandan strain virus. Fully engorged mosquitoes were maintained in an environmental chamber set at 29°C and 80-85%RH. Twelve mosquitoes were then sampled daily from day one to seven and on day 10 and 14 post infection (pi). Zika virus titre in the midgut and salivary glands of each mosquito were determined using tissue culture infectious dose50 assay, while transmissibility of the virus was determined by detecting viral antigen in the mosquito saliva by qRT-PCR. High dissemination and transmission rate of ZIKV were observed. By day 7-pi, all mosquitoes have disseminated infection and 73% of these mosquitoes have ZIKV in their saliva. By day 10-pi, all mosquitoes were potentially infectious.The study highlighted the potential of Ae. albopictus to transmit ZIKV and the possibility that the virus could be established locally. Nonetheless, the threat of ZIKV can be mitigated by existing dengue and chikungunya control program being implemented in Singapore."],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."],"language":["eng"],"pubModel":["Electronic-Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Gastrointestinal Tract"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Salivary Glands"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Saliva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Load"]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Singapore"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]},{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3731215?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3731215"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23936579"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23936579&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0002348"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["15"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-02-28"],"dateOfCreation":["2013-08-12"],"dateOfRevision":["2016-02-05"],"electronicPublicationDate":["2013-08-01"],"firstPublicationDate":["2013-08-01"],"cprojectID":"PMC3731215"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsoJ9hGurOzZIYa","_score":1,"_source":{"id":["26366194"],"source":["MED"],"pmid":["26366194"],"pmcid":["PMC4561566"],"doi":["10.1111/eva.12286"],"title":["Wolbachia strains for disease control: ecological and evolutionary considerations."],"authorString":["Hoffmann AA, Ross PA, Rašić G."],"authorList":[{"author":[{"fullName":["Hoffmann AA"],"firstName":["Ary A"],"lastName":["Hoffmann"],"initials":["AA"],"affiliation":["Pest and Environmental Adaptation Research Group, School of BioSciences, Bio21 Institute, The University of Melbourne Parkville, Vic., Australia."]},{"fullName":["Ross PA"],"firstName":["Perran A"],"lastName":["Ross"],"initials":["PA"],"affiliation":["Pest and Environmental Adaptation Research Group, School of BioSciences, Bio21 Institute, The University of Melbourne Parkville, Vic., Australia."]},{"fullName":["Rašić G"],"firstName":["Gordana"],"lastName":["Rašić"],"initials":["G"],"authorId":[{"_":"0000-0002-3057-0093","$":{"type":"ORCID"}}],"affiliation":["Pest and Environmental Adaptation Research Group, School of BioSciences, Bio21 Institute, The University of Melbourne Parkville, Vic., Australia."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3057-0093","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["8"],"journalIssueId":["2323665"],"dateOfPublication":["2015 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2015"],"printPublicationDate":["2015-09-01"],"journal":[{"title":["Evolutionary applications"],"ISOAbbreviation":["Evol Appl"],"medlineAbbreviation":["Evol Appl"],"NLMid":["101461828"],"ESSN":["1752-4571"]}]}],"pubYear":["2015"],"pageInfo":["751-768"],"abstractText":["Wolbachia are endosymbionts found in many insects with the potential to suppress vectorborne diseases, particularly through interfering with pathogen transmission. Wolbachia strains are highly variable in their effects on hosts, raising the issue of which attributes should be selected to ensure that the best strains are developed for disease control. This depends on their ability to suppress viral transmission, invade host populations, persist without loss of viral suppression and not interfere with other control strategies. The potential to achieve these objectives is likely to involve evolutionary constraints; viral suppression may be limited by the ability of infections to spread due to deleterious host fitness effects. However, there are exceptions to these patterns in both natural infections and in novel associations generated following interspecific transfer, suggesting that pathogen blockage, deleterious fitness effects and changes to reproductive biology might be at least partly decoupled to achieve ideal infection attributes. The stability of introduced Wolbachia and its effects on viral transmission remain unclear, but rapid evolutionary changes seem unlikely. Although deliberate transfers of Wolbachia across species remain particularly challenging, the availability of strains with desirable attributes should be expanded, taking advantage of the diversity available across thousands of strains in natural populations."],"affiliation":["Pest and Environmental Adaptation Research Group, School of BioSciences, Bio21 Institute, The University of Melbourne Parkville, Vic., Australia."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Aedes","Virus","Fitness","Wolbachia","Disease control","Deployment Issues","Strain Attributes"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4561566?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4561566"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1111/eva.12286"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["5"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-09-14"],"dateOfCreation":["2015-09-14"],"dateOfRevision":["2015-09-16"],"electronicPublicationDate":["2015-07-20"],"firstPublicationDate":["2015-07-20"],"cprojectID":"PMC4561566"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSd_8VqgcuJwvf3","_score":1,"_source":{"id":["24516683"],"source":["MED"],"pmid":["24516683"],"pmcid":["PMC3916288"],"doi":["10.1371/journal.pntd.0002681"],"title":["Zika virus in Gabon (Central Africa)--2007: a new threat from Aedes albopictus?"],"authorString":["Grard G, Caron M, Mombo IM, Nkoghe D, Mboui Ondo S, Jiolle D, Fontenille D, Paupy C, Leroy EM."],"authorList":[{"author":[{"fullName":["Grard G"],"firstName":["Gilda"],"lastName":["Grard"],"initials":["G"],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon."]},{"fullName":["Caron M"],"firstName":["Mélanie"],"lastName":["Caron"],"initials":["M"],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon ; MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France."]},{"fullName":["Mombo IM"],"firstName":["Illich Manfred"],"lastName":["Mombo"],"initials":["IM"],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon ; MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France."]},{"fullName":["Nkoghe D"],"firstName":["Dieudonné"],"lastName":["Nkoghe"],"initials":["D"],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon ; Ministère de la Santé Publique, Libreville, Gabon."]},{"fullName":["Mboui Ondo S"],"firstName":["Statiana"],"lastName":["Mboui Ondo"],"initials":["S"],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon."]},{"fullName":["Jiolle D"],"firstName":["Davy"],"lastName":["Jiolle"],"initials":["D"],"affiliation":["MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France ; URES, CIRMF, Franceville, Gabon."]},{"fullName":["Fontenille D"],"firstName":["Didier"],"lastName":["Fontenille"],"initials":["D"],"affiliation":["MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France."]},{"fullName":["Paupy C"],"firstName":["Christophe"],"lastName":["Paupy"],"initials":["C"],"affiliation":["MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France ; URES, CIRMF, Franceville, Gabon."]},{"fullName":["Leroy EM"],"firstName":["Eric Maurice"],"lastName":["Leroy"],"initials":["EM"],"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}}],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon ; MIVEGEC, Institut de Recherche pour le Développement (IRD-224, CNRS-5290, Universités de Montpellier 1 & 2), Montpellier, France."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["8"],"journalIssueId":["2125944"],"dateOfPublication":["2014 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2014"],"printPublicationDate":["2014-02-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2014"],"pageInfo":["e2681"],"abstractText":["Chikungunya and dengue viruses emerged in Gabon in 2007, with large outbreaks primarily affecting the capital Libreville and several northern towns. Both viruses subsequently spread to the south-east of the country, with new outbreaks occurring in 2010. The mosquito species Aedes albopictus, that was known as a secondary vector for both viruses, recently invaded the country and was the primary vector involved in the Gabonese outbreaks. We conducted a retrospective study of human sera and mosquitoes collected in Gabon from 2007 to 2010, in order to identify other circulating arboviruses.Sample collections, including 4312 sera from patients presenting with painful febrile disease, and 4665 mosquitoes belonging to 9 species, split into 247 pools (including 137 pools of Aedes albopictus), were screened with molecular biology methods. Five human sera and two Aedes albopictus pools, all sampled in an urban setting during the 2007 outbreak, were positive for the flavivirus Zika (ZIKV). The ratio of Aedes albopictus pools positive for ZIKV was similar to that positive for dengue virus during the concomitant dengue outbreak suggesting similar mosquito infection rates and, presumably, underlying a human ZIKV outbreak. ZIKV sequences from the envelope and NS3 genes were amplified from a human serum sample. Phylogenetic analysis placed the Gabonese ZIKV at a basal position in the African lineage, pointing to ancestral genetic diversification and spread.We provide the first direct evidence of human ZIKV infections in Gabon, and its first occurrence in the Asian tiger mosquito, Aedes albopictus. These data reveal an unusual natural life cycle for this virus, occurring in an urban environment, and potentially representing a new emerging threat due to this novel association with a highly invasive vector whose geographic range is still expanding across the globe."],"affiliation":["UMVE, Centre International de Recherches Médicales de Franceville, Franceville, Gabon."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Retrospective Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Gabon"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3916288?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3916288"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24516683&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24516683"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0002681"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["29"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2014-11-03"],"dateOfCreation":["2014-02-11"],"dateOfRevision":["2016-02-04"],"electronicPublicationDate":["2014-02-06"],"firstPublicationDate":["2014-02-06"],"cprojectID":"PMC3916288"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqxlJ9hGurOzZIYn","_score":1,"_source":{"id":["26549900"],"source":["MED"],"pmid":["26549900"],"pmcid":["PMC4622157"],"doi":["10.2471/blt.15.021115"],"title":["Averting climate change's health effects in Fiji."],"journalInfo":[{"issue":["11"],"volume":["93"],"journalIssueId":["2341451"],"dateOfPublication":["2015 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2015"],"printPublicationDate":["2015-11-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2015"],"pageInfo":["746-747"],"abstractText":["Pacific islanders face up to the dire health effects of global warming. Atasa Moceituba and Monique Tsang report."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4622157?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4622157"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.15.021115"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-11-09"],"dateOfRevision":["2015-11-12"],"firstPublicationDate":["2015-11-01"],"cprojectID":"PMC4622157"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqxv_8VqgcuJwvgo","_score":1,"_source":{"id":["25885461"],"source":["MED"],"pmid":["25885461"],"pmcid":["PMC4381565"],"doi":["10.1186/s13071-015-0808-3"],"title":["Invasion of Aedes albopictus (Diptera: Culicidae) into central Africa: what consequences for emerging diseases?"],"authorString":["Ngoagouni C, Kamgang B, Nakouné E, Paupy C, Kazanji M."],"authorList":[{"author":[{"fullName":["Ngoagouni C"],"firstName":["Carine"],"lastName":["Ngoagouni"],"initials":["C"],"affiliation":["Institut Pasteur de Bangui, Bangui, Central African Republic. ngoagounic@yahoo.fr."]},{"fullName":["Kamgang B"],"firstName":["Basile"],"lastName":["Kamgang"],"initials":["B"],"affiliation":["Institut Pasteur de Bangui, Bangui, Central African Republic. kamgang_d@yahoo.fr."]},{"fullName":["Nakouné E"],"firstName":["Emmanuel"],"lastName":["Nakouné"],"initials":["E"],"affiliation":["Institut Pasteur de Bangui, Bangui, Central African Republic. enakouney@gmail.com."]},{"fullName":["Paupy C"],"firstName":["Chistophe"],"lastName":["Paupy"],"initials":["C"],"affiliation":["Laboratoire des Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle, UMR 224-5290, CNRS-IRD-UM1-UM2, IRD, Montpellier, France. Christophe.paupy@ird.fr."]},{"fullName":["Kazanji M"],"firstName":["Mirdad"],"lastName":["Kazanji"],"initials":["M"],"affiliation":["Institut Pasteur de Bangui, Bangui, Central African Republic. mirdad.kazanji@pasteur.fr."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2261389"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2015"],"pageInfo":["191"],"abstractText":["Aedes albopictus, a mosquito native to Asia, has invaded all five continents during the past three decades. It was reported in central Africa in the 2000s, first in Cameroon, and, since then, has colonised almost all countries of the region. The species, originally considered a secondary vector of dengue viruses, has been showed to play a major role in transmission of chikungunya virus in numerous countries, including in the central African region. We review the current spread of Ae. albopictus in central Africa, its larval ecology and its impact on indigenous species such as Ae. aegypti. We explore the potential of Ae. albopictus to affect the epidemiology of emerging or re-emerging arboviruses and discuss the conventional means for its control, while emphasizing the importance of data on its susceptibility to insecticides to cope with potential outbreaks."],"affiliation":["Institut Pasteur de Bangui, Bangui, Central African Republic. ngoagounic@yahoo.fr."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Animal Population Groups"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Arbovirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Ecosystem"]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Africa, Central"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4381565?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4381565"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-015-0808-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["5"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-01-04"],"dateOfCreation":["2015-04-18"],"dateOfRevision":["2015-04-20"],"electronicPublicationDate":["2015-03-31"],"firstPublicationDate":["2015-03-31"],"cprojectID":"PMC4381565"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqyKJ9hGurOzZIYq","_score":1,"_source":{"id":["26580074"],"source":["MED"],"pmid":["26580074"],"pmcid":["PMC4651505"],"doi":["10.1371/journal.pntd.0004199"],"title":["Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases."],"authorString":["Sahadeo N, Mohammed H, Allicock OM, Auguste AJ, Widen SG, Badal K, Pulchan K, Foster JE, Weaver SC, Carrington CV."],"authorList":[{"author":[{"fullName":["Sahadeo N"],"firstName":["Nikita"],"lastName":["Sahadeo"],"initials":["N"],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."]},{"fullName":["Mohammed H"],"firstName":["Hamish"],"lastName":["Mohammed"],"initials":["H"],"affiliation":["Public Health England, HIV & STI Department, London, United Kingdom."]},{"fullName":["Allicock OM"],"firstName":["Orchid M"],"lastName":["Allicock"],"initials":["OM"],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."]},{"fullName":["Auguste AJ"],"firstName":["Albert J"],"lastName":["Auguste"],"initials":["AJ"],"affiliation":["Institute for Human Infections and Immunity and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America."]},{"fullName":["Widen SG"],"firstName":["Steven G"],"lastName":["Widen"],"initials":["SG"],"affiliation":["Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, United States of America."]},{"fullName":["Badal K"],"firstName":["Kimberly"],"lastName":["Badal"],"initials":["K"],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."]},{"fullName":["Pulchan K"],"firstName":["Krishna"],"lastName":["Pulchan"],"initials":["K"],"affiliation":["Adult Emergency Department, Eric Williams Medical Sciences Complex, Mt. Hope, Republic of Trinidad and Tobago."]},{"fullName":["Foster JE"],"firstName":["Jerome E"],"lastName":["Foster"],"initials":["JE"],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Institute for Human Infections and Immunity and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America."]},{"fullName":["Carrington CV"],"firstName":["Christine V F"],"lastName":["Carrington"],"initials":["CV"],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."]}]}],"journalInfo":[{"issue":["11"],"volume":["9"],"journalIssueId":["2338334"],"dateOfPublication":["2015 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2015"],"printPublicationDate":["2015-11-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004199"],"abstractText":["Local transmission of Chikungunya virus (CHIKV) was first documented in Trinidad and Tobago (T&T) in July 2014 preceding a large epidemic. At initial presentation, it is difficult to distinguish chikungunya fever (CHIKF) from other acute undifferentiated febrile illnesses (AUFIs), including life-threatening dengue disease. We characterised and compared dengue virus (DENV) and CHIKV infections in 158 patients presenting with suspected dengue fever (DF) and CHIKF at a major hospital in T&T, and performed phylogenetic analyses on CHIKV genomic sequences recovered from 8 individuals. The characteristics of patients with and without PCR-confirmed CHIKV were compared using Pearson's χ2 and student's t-tests, and adjusted odds ratios (aORs) and 95% confidence intervals (CIs) were determined using logistic regression. We then compared signs and symptoms of people with RT-qPCR-confirmed CHIKV and DENV infections using the Mann-Whitney U, Pearson's χ2 and Fisher's exact tests. Among the 158 persons there were 8 (6%) RT-qPCR-confirmed DENV and 30 (22%) RT-qPCR-confirmed CHIKV infections. Phylogenetic analyses showed that the CHIKV strains belonged to the Asian genotype and were most closely related to a British Virgin Islands strain isolated at the beginning of the 2013/14 outbreak in the Americas. Compared to persons who were RT-qPCR-negative for CHIKV, RT-qPCR-positive individuals were significantly more likely to have joint pain (aOR: 4.52 [95% CI: 1.28-16.00]), less likely to be interviewed at a later stage of illness (days post onset of fever--aOR: 0.56 [0.40-0.78]) and had a lower white blood cell count (aOR: 0.83 [0.71-0.96]). Among the 38 patients with RT-qPCR-confirmed CHIKV or DENV, there were no significant differences in symptomatic presentation. However when individuals with serological evidence of recent DENV or CHIKV infection were included in the analyses, there were key differences in clinical presentation between CHIKF and other AUFIs including DF, which can be used to triage patients for appropriate care in the clinical setting."],"affiliation":["Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Republic of Trinidad and Tobago."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, N.I.H., Extramural","Comparative Study"]}],"grantsList":[{"grant":[{"grantId":["AI093491"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Fever of Unknown Origin"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Diagnosis, Differential"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Diagnostic Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Homology"]},{"majorTopic_YN":["N"],"descriptorName":["Genotype"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Aged, 80 and over"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Trinidad and Tobago"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4651505?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4651505"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004199"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["26656103"],"source":["MED"],"reference":["PLoS Negl Trop Dis. 2015 Dec;9(12):e0004305"],"type":["Erratum in"],"orderIn":["43"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-13"],"dateOfCreation":["2015-11-20"],"dateOfRevision":["2015-11-26"],"electronicPublicationDate":["2015-11-18"],"firstPublicationDate":["2015-11-18"],"cprojectID":"PMC4651505"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq13_8VqgcuJwvgw","_score":1,"_source":{"id":["26217340"],"source":["MED"],"pmid":["26217340"],"pmcid":["PMC4495344"],"doi":["10.3389/fimmu.2015.00348"],"title":["Preclinical Assessment of Viral Vectored and Protein Vaccines Targeting the Duffy-Binding Protein Region II of Plasmodium Vivax."],"authorString":["de Cassan SC, Shakri AR, Llewellyn D, Elias SC, Cho JS, Goodman AL, Jin J, Douglas AD, Suwanarusk R, Nosten FH, Rénia L, Russell B, Chitnis CE, Draper SJ."],"authorList":[{"author":[{"fullName":["de Cassan SC"],"firstName":["Simone C"],"lastName":["de Cassan"],"initials":["SC"],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Shakri AR"],"firstName":["A Rushdi"],"lastName":["Shakri"],"initials":["AR"],"affiliation":["International Center for Genetic Engineering and Biotechnology , New Delhi , India."]},{"fullName":["Llewellyn D"],"firstName":["David"],"lastName":["Llewellyn"],"initials":["D"],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Elias SC"],"firstName":["Sean C"],"lastName":["Elias"],"initials":["SC"],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Cho JS"],"firstName":["Jee Sun"],"lastName":["Cho"],"initials":["JS"],"affiliation":["Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore , Singapore , Singapore ; Singapore Immunology Network, Agency for Science, Technology and Research (ASTAR) , Singapore , Singapore."]},{"fullName":["Goodman AL"],"firstName":["Anna L"],"lastName":["Goodman"],"initials":["AL"],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Jin J"],"firstName":["Jing"],"lastName":["Jin"],"initials":["J"],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Douglas AD"],"firstName":["Alexander D"],"lastName":["Douglas"],"initials":["AD"],"authorId":[{"_":"0000-0002-5410-7562","$":{"type":"ORCID"}}],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]},{"fullName":["Suwanarusk R"],"firstName":["Rossarin"],"lastName":["Suwanarusk"],"initials":["R"],"affiliation":["Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore , Singapore , Singapore ; Singapore Immunology Network, Agency for Science, Technology and Research (ASTAR) , Singapore , Singapore."]},{"fullName":["Nosten FH"],"firstName":["François H"],"lastName":["Nosten"],"initials":["FH"],"affiliation":["Shoklo Malaria Research Unit (SMRU), Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University , Mae Sot , Thailand."]},{"fullName":["Rénia L"],"firstName":["Laurent"],"lastName":["Rénia"],"initials":["L"],"affiliation":["Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore , Singapore , Singapore ; Singapore Immunology Network, Agency for Science, Technology and Research (ASTAR) , Singapore , Singapore."]},{"fullName":["Russell B"],"firstName":["Bruce"],"lastName":["Russell"],"initials":["B"],"affiliation":["Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore , Singapore , Singapore."]},{"fullName":["Chitnis CE"],"firstName":["Chetan E"],"lastName":["Chitnis"],"initials":["CE"],"affiliation":["International Center for Genetic Engineering and Biotechnology , New Delhi , India."]},{"fullName":["Draper SJ"],"firstName":["Simon J"],"lastName":["Draper"],"initials":["SJ"],"authorId":[{"_":"0000-0002-9415-1357","$":{"type":"ORCID"}}],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5410-7562","$":{"type":"ORCID"}},{"_":"0000-0002-9415-1357","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2260597"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Frontiers in immunology"],"ISOAbbreviation":["Front Immunol"],"medlineAbbreviation":["Front Immunol"],"NLMid":["101560960"],"ESSN":["1664-3224"]}]}],"pubYear":["2015"],"pageInfo":["348"],"abstractText":["Malaria vaccine development has largely focused on Plasmodium falciparum; however, a reawakening to the importance of Plasmodium vivax has spurred efforts to develop vaccines against this difficult to treat and at times severe form of relapsing malaria, which constitutes a significant proportion of human malaria cases worldwide. The almost complete dependence of P. vivax red blood cell invasion on the interaction of the P. vivax Duffy-binding protein region II (PvDBP_RII) with the human Duffy antigen receptor for chemokines (DARC) makes this antigen an attractive vaccine candidate against blood-stage P. vivax. Here, we generated both preclinical and clinically compatible adenoviral and poxviral vectored vaccine candidates expressing the Salvador I allele of PvDBP_RII - including human adenovirus serotype 5 (HAdV5), chimpanzee adenovirus serotype 63 (ChAd63), and modified vaccinia virus Ankara (MVA) vectors. We report on the antibody and T cell immunogenicity of these vaccines in mice or rabbits, either used alone in a viral vectored prime-boost regime or in \"mixed-modality\" adenovirus prime - protein-in--adjuvant boost regimes (using a recombinant PvDBP_RII protein antigen formulated in Montanide(®)ISA720 or Abisco(®)100 adjuvants). Antibodies induced by these regimes were found to bind to native parasite antigen from P. vivax infected Thai patients and were capable of inhibiting the binding of PvDBP_RII to its receptor DARC using an in vitro binding inhibition assay. In recent years, recombinant ChAd63 and MVA vectors have been quickly translated into human clinical trials for numerous antigens from P. falciparum as well as a growing number of other pathogens. The vectors reported here are immunogenic in small animals, elicit antibodies against PvDBP_RII, and have recently entered clinical trials, which will provide the first assessment of the safety and immunogenicity of the PvDBP_RII antigen in humans."],"affiliation":["The Jenner Institute, University of Oxford , Oxford , UK."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["G0600424"],"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["G1100086"],"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["G1000527"],"agency":["Medical Research Council"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Poxvirus","Malaria","Adenovirus","Vaccine","Plasmodium vivax","Mva","Blood-stage","Duffy-binding Protein"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4495344?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4495344"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fimmu.2015.00348"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," nct"," "]}],"dateOfCompletion":["2015-07-28"],"dateOfCreation":["2015-07-28"],"dateOfRevision":["2015-12-22"],"electronicPublicationDate":["2015-07-08"],"firstPublicationDate":["2015-07-08"],"cprojectID":"PMC4495344"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq3gJ9hGurOzZIYx","_score":1,"_source":{"id":["26839785"],"source":["MED"],"pmid":["26839785"],"pmcid":["PMC4735082"],"doi":["10.1016/j.idcr.2014.10.009"],"title":["Trouble in paradise."],"authorString":["Brust KB, Prince WS, Fader RC."],"authorList":[{"author":[{"fullName":["Brust KB"],"firstName":["Karen B"],"lastName":["Brust"],"initials":["KB"],"affiliation":["Division of Infectious Diseases, Baylor Scott & White Healthcare, Texas A&M Health Science Center College of Medicine, Temple, TX, USA."]},{"fullName":["Prince WS"],"firstName":["Whitney S"],"lastName":["Prince"],"initials":["WS"],"affiliation":["Division of Pulmonary, Baylor Scott & White Healthcare, Texas A&M Health Science Center College of Medicine, Temple, TX, USA."]},{"fullName":["Fader RC"],"firstName":["Robert C"],"lastName":["Fader"],"initials":["RC"],"affiliation":["Division of Microbiology, Baylor Scott & White Healthcare, Temple, TX, USA."]}]}],"journalInfo":[{"issue":["4"],"volume":["1"],"journalIssueId":["2370433"],"dateOfPublication":["2014"],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["IDCases"],"ISOAbbreviation":["IDCases"],"medlineAbbreviation":["IDCases"],"NLMid":["101634540"],"ESSN":["2214-2509"]}]}],"pubYear":["2014"],"pageInfo":["95-96"],"affiliation":["Division of Infectious Diseases, Baylor Scott & White Healthcare, Texas A&M Health Science Center College of Medicine, Temple, TX, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["outbreak","Flavivirus","Zika Fever","Fever In A Traveler"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4735082?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4735082"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.idcr.2014.10.009"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-02-03"],"dateOfCreation":["2016-02-03"],"dateOfRevision":["2016-02-08"],"electronicPublicationDate":["2014-11-03"],"firstPublicationDate":["2014-11-03"],"cprojectID":"PMC4735082"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq4bhOUS2orL1IPA","_score":1,"_source":{"id":["26784852"],"source":["MED"],"pmid":["26784852"],"pmcid":["PMC4727432"],"doi":["10.1590/0074-02760150270"],"title":["Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiaba, state of Mato Grosso, Brazil."],"authorString":["Serra OP, Cardoso BF, Ribeiro AL, Santos FA, Slhessarenko RD."],"authorList":[{"author":[{"fullName":["Serra OP"],"firstName":["Otacília Pereira"],"lastName":["Serra"],"initials":["OP"],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."]},{"fullName":["Cardoso BF"],"firstName":["Belgath Fernandes"],"lastName":["Cardoso"],"initials":["BF"],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."]},{"fullName":["Ribeiro AL"],"firstName":["Ana Lúcia Maria"],"lastName":["Ribeiro"],"initials":["AL"],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."]},{"fullName":["Santos FA"],"firstName":["Fábio Alexandre Leal dos"],"lastName":["Santos"],"initials":["FA"],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."]},{"fullName":["Slhessarenko RD"],"firstName":["Renata Dezengrini"],"lastName":["Slhessarenko"],"initials":["RD"],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."]}]}],"journalInfo":[{"issue":["1"],"volume":["111"],"journalIssueId":["2367659"],"dateOfPublication":["2016 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["20-29"],"abstractText":["This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (RT-PCR) for 11 flavivirus and five alphavirus. Positive pools were tested by single RT-PCR followed by nucleotide sequencing, by RT-PCR for E1 gene [Mayaro virus (MAYV)] and by inoculation in Vero cells (MAYV) or C6/36 cells (flaviviruses). One/171 Aedes aegypti was positive for dengue virus (DENV)-1, 12/403 Culex quinquefasciatus, and four/171Ae. aegypti for MAYV, which was isolated from two pools containing two nonengorged females of Ae. aegypti and two of Cx. quinquefasciatus. DENV-4 was detected in 58/171 pools of Ae. aegytpi, 105/403 Cx. quinquefasciatus, two/five Psorophora sp., two/11 Psorophora varipes/Psorophora albigenu, one/one Sabethes chloropterus, two/five Culex bidens/Culex interfor, and one/one Aedes sp. DENV-4 was isolated from two pools containing three and 16 nonengorged Cx. quinquefasciatus females. Phylogenetic analysis revealed MAYV belongs to genotype L, clustering with human samples of the virus previously identified in the city. Cuiabá has biodiversity and ecosystem favourable for vector proliferation, representing a risk for arbovirus outbreaks."],"affiliation":["Faculdade de Medicina, Centro de Ciências Básicas da Saúde I, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Alphavirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"]},{"majorTopic_YN":["N"],"descriptorName":["Seasons"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Genotype"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Multiplex Polymerase Chain Reaction"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4727432?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4727432"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760150270"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-08"],"dateOfCreation":["2016-01-28"],"dateOfRevision":["2016-01-28"],"firstPublicationDate":["2016-01-08"],"cprojectID":"PMC4727432"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq8d_8VqgcuJwvg4","_score":1,"_source":{"id":["26413514"],"source":["MED"],"pmid":["26413514"],"pmcid":["PMC4568054"],"doi":["10.1155/2015/321873"],"title":["Epidemiological Scenario of Dengue in Brazil."],"authorString":["Fares RC, Souza KP, Añez G, Rios M."],"authorList":[{"author":[{"fullName":["Fares RC"],"firstName":["Rafaelle C G"],"lastName":["Fares"],"initials":["RC"],"affiliation":["United States Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD 20993, USA."]},{"fullName":["Souza KP"],"firstName":["Katia P R"],"lastName":["Souza"],"initials":["KP"],"affiliation":["United States Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD 20993, USA."]},{"fullName":["Añez G"],"firstName":["Germán"],"lastName":["Añez"],"initials":["G"],"authorId":[{"_":"0000-0001-5361-3001","$":{"type":"ORCID"}}],"affiliation":["United States Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD 20993, USA."]},{"fullName":["Rios M"],"firstName":["Maria"],"lastName":["Rios"],"initials":["M"],"affiliation":["United States Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD 20993, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-5361-3001","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["2015"],"journalIssueId":["2246988"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BioMed research international"],"ISOAbbreviation":["Biomed Res Int"],"medlineAbbreviation":["Biomed Res Int"],"NLMid":["101600173"],"ISSN":["2314-6133"],"ESSN":["2314-6141"]}]}],"pubYear":["2015"],"pageInfo":["321873"],"abstractText":["Dengue is the most important reemerging mosquito-borne viral disease worldwide. It is caused by any of four Dengue virus types or serotypes (DENV-1 to DENV-4) and is transmitted by mosquitoes from the genus Aedes. Ecological changes have favored the geographic expansion of the vector and, since the dengue pandemic in the Asian and Pacific regions, the infection became widely distributed worldwide, reaching Brazil in 1845. The incidence of dengue in Brazil has been frequently high, and the number of cases in the country has at some point in time represented up to 60% of the dengue reported cases worldwide. This review addresses vector distribution, dengue outbreaks, circulating serotypes and genotypes, and prevention approaches being utilized in Brazil."],"affiliation":["United States Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, MD 20993, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Incidence"]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4568054?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4568054"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2015/321873"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2015-09-28"],"dateOfRevision":["2015-10-01"],"electronicPublicationDate":["2015-08-30"],"firstPublicationDate":["2015-08-30"],"cprojectID":"PMC4568054"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9T_8VqgcuJwvg-","_score":1,"_source":{"id":["26583130"],"source":["MED"],"pmid":["26583130"],"pmcid":["PMC4637084"],"doi":["10.1155/2015/763715"],"title":["Importation and Transmission of Parasitic and Other Infectious Diseases Associated with International Adoptees and Refugees Immigrating into the United States of America."],"authorString":["Smith Darr J, Conn DB."],"authorList":[{"author":[{"fullName":["Smith Darr J"],"firstName":["Jordan"],"lastName":["Smith Darr"],"initials":["J"],"affiliation":["One Health Center and Department of Biology, Berry College, Mount Berry, GA 30149, USA."]},{"fullName":["Conn DB"],"firstName":["David Bruce"],"lastName":["Conn"],"initials":["DB"],"authorId":[{"_":"0000-0003-0198-0234","$":{"type":"ORCID"}}],"affiliation":["One Health Center and Department of Biology, Berry College, Mount Berry, GA 30149, USA ; Department of Invertebrate Zoology, Museum of Comparative Zoology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-0198-0234","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["2015"],"journalIssueId":["2246988"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BioMed research international"],"ISOAbbreviation":["Biomed Res Int"],"medlineAbbreviation":["Biomed Res Int"],"NLMid":["101600173"],"ISSN":["2314-6133"],"ESSN":["2314-6141"]}]}],"pubYear":["2015"],"pageInfo":["763715"],"abstractText":["Each year, hundreds of millions of people travel across international borders or even oceans, and up to 230 million may remain for long periods. Among these, 3-5 million settle permanently in their new homes, with about 1 million migrating permanently to the United States of America. This may result in transport of parasites and other pathogens, which might become established, infecting individuals in the new location. Beyond concern of disease spread, the health of migrants is of concern since the rigors, circumstances, and living conditions surrounding migrations may increase the vulnerability of migrants to infections. International adoptees and refugees are a small subset of these migrants but are of special significance inasmuch as adoptees may be more vulnerable to infection due to their immature immune status, and refugees may be more vulnerable due to substandard living conditions. Both originate from diverse regions, but often from environments of low hygiene and health care standards. This review examines recent examples of infections reported from adoptees and refugees entering the USA through 2010, highlighting the most common origin countries and the diseases most frequently involved, including Chagas disease, Balamuthia amebic meningoencephalitis, giardiasis, microsporidiosis, hepatitis, measles, pertussis, tuberculosis, malaria, intestinal helminths, and syphilis."],"affiliation":["One Health Center and Department of Biology, Berry College, Mount Berry, GA 30149, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["N"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Refugees"]},{"majorTopic_YN":["N"],"descriptorName":["United States"]},{"majorTopic_YN":["Y"],"descriptorName":["Human Migration"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4637084?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4637084"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2015/763715"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-09-06"],"dateOfCreation":["2015-11-20"],"dateOfRevision":["2015-11-23"],"electronicPublicationDate":["2015-10-25"],"firstPublicationDate":["2015-10-25"],"cprojectID":"PMC4637084"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9VJ9hGurOzZIZB","_score":1,"_source":{"id":["27013839"],"source":["MED"],"pmid":["27013839"],"pmcid":["PMC4785754"],"doi":["10.4103/0974-777x.176140"],"title":["The Emergence of Zika Virus as a Global Health Security Threat: A Review and a Consensus Statement of the INDUSEM Joint working Group (JWG)."],"authorString":["Sikka V, Chattu VK, Popli RK, Galwankar SC, Kelkar D, Sawicki SG, Stawicki SP, Papadimos TJ."],"authorList":[{"author":[{"fullName":["Sikka V"],"firstName":["Veronica"],"lastName":["Sikka"],"initials":["V"],"affiliation":["Department of Emergency Medicine, Veterans Affairs Medical Center, Orlando, USA."]},{"fullName":["Chattu VK"],"firstName":["Vijay Kumar"],"lastName":["Chattu"],"initials":["VK"],"affiliation":["Institute for International Relations, The University of West Indies, St. Augustine, Trinidad and Tobago, USA."]},{"fullName":["Popli RK"],"firstName":["Raaj K"],"lastName":["Popli"],"initials":["RK"],"affiliation":["Digestive Disease Consultants of Central Florida, Altamonte Springs, Florida, USA."]},{"fullName":["Galwankar SC"],"firstName":["Sagar C"],"lastName":["Galwankar"],"initials":["SC"],"affiliation":["Department of Emergency Medicine, University of Florida, Jacksonville, Florida, USA."]},{"fullName":["Kelkar D"],"firstName":["Dhanashree"],"lastName":["Kelkar"],"initials":["D"],"affiliation":["Department of Emergency Medicine, University of Florida, Jacksonville, Florida, USA."]},{"fullName":["Sawicki SG"],"firstName":["Stanley G"],"lastName":["Sawicki"],"initials":["SG"],"affiliation":["Department of Medical Microbiology and Immunology, College of Medicine and the Life Sciences, University of Toledo, Toledo, USA."]},{"fullName":["Stawicki SP"],"firstName":["Stanislaw P"],"lastName":["Stawicki"],"initials":["SP"],"affiliation":["Department of Research and Innovation, St. Luke's University Health Network, Bethlehem, Pennsylvania, USA."]},{"fullName":["Papadimos TJ"],"firstName":["Thomas J"],"lastName":["Papadimos"],"initials":["TJ"],"affiliation":["Department of Anesthesiology, The Ohio State University College of Medicine, Columbus, Ohio, USA."]}]}],"journalInfo":[{"issue":["1"],"volume":["8"],"journalIssueId":["2390792"],"dateOfPublication":["2016 Jan-Mar"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of global infectious diseases"],"ISOAbbreviation":["J Glob Infect Dis"],"medlineAbbreviation":["J Glob Infect Dis"],"NLMid":["101521436"],"ISSN":["0974-777X"],"ESSN":["0974-8245"]}]}],"pubYear":["2016"],"pageInfo":["3-15"],"abstractText":["The Zika virus (ZIKV), first discovered in 1947, has emerged as a global public health threat over the last decade, with the accelerated geographic spread of the virus noted during the last 5 years. The World Health Organization (WHO) predicts that millions of cases of ZIKV are likely to occur in the Americas during the next 12 months. These projections, in conjunction with suspected Zika-associated increase in newborn microcephaly cases, prompted WHO to declare public health emergency of international concern. ZIKV-associated illness is characterized by an incubation period of 3-12 days. Most patients remain asymptomatic (i.e., ~80%) after contracting the virus. When symptomatic, clinical presentation is usually mild and consists of a self-limiting febrile illness that lasts approximately 2-7 days. Among common clinical manifestations are fever, arthralgia, conjunctivitis, myalgia, headache, and maculopapular rash. Hospitalization and complication rates are low, with fatalities being extremely rare. Newborn microcephaly, the most devastating and insidious complication associated with the ZIKV, has been described in the offspring of women who became infected while pregnant. Much remains to be elucidated about the timing of ZIKV infection in the context of the temporal progression of pregnancy, the corresponding in utero fetal development stage(s), and the risk of microcephaly. Without further knowledge of the pathophysiology involved, the true risk of ZIKV to the unborn remains difficult to quantify and remediate. Accurate, portable, and inexpensive point-of-care testing is required to better identify cases and manage the current and future outbreaks of ZIKV, including optimization of preventive approaches and the identification of more effective risk reduction strategies. In addition, much more work needs to be done to produce an effective vaccine. Given the rapid geographic spread of ZIKV in recent years, a coordinated local, regional, and global effort is needed to generate sufficient resources and political traction to effectively halt and contain further expansion of the current outbreak."],"affiliation":["Department of Emergency Medicine, Veterans Affairs Medical Center, Orlando, USA."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Public Health","Arbovirus","Flavivirus","Global Health Security","Zika Virus","Public Health Emergency Of International Concern","Viral Vector Control"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4785754"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0974-777X.176140"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-03-25"],"dateOfCreation":["2016-03-25"],"dateOfRevision":["2016-03-27"],"firstPublicationDate":["2016-01-01"],"cprojectID":"PMC4785754"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-3_8VqgcuJwvhD","_score":1,"_source":{"id":["26799213"],"source":["MED"],"pmid":["26799213"],"pmcid":["PMC4723151"],"doi":["10.1371/journal.pntd.0004374"],"title":["Genetic Diversity and Phylogeny of Aedes aegypti, the Main Arbovirus Vector in the Pacific."],"authorString":["Calvez E, Guillaumot L, Millet L, Marie J, Bossin H, Rama V, Faamoe A, Kilama S, Teurlai M, Mathieu-Daudé F, Dupont-Rouzeyrol M."],"authorList":[{"author":[{"fullName":["Calvez E"],"firstName":["Elodie"],"lastName":["Calvez"],"initials":["E"],"affiliation":["URE-Dengue et autres Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."]},{"fullName":["Guillaumot L"],"firstName":["Laurent"],"lastName":["Guillaumot"],"initials":["L"],"affiliation":["URE-Entomologie Médicale, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."]},{"fullName":["Millet L"],"firstName":["Laurent"],"lastName":["Millet"],"initials":["L"],"affiliation":["UMR 9220, ENTROPIE, Institut de Recherche pour le Développement, Noumea, New Caledonia."]},{"fullName":["Marie J"],"firstName":["Jérôme"],"lastName":["Marie"],"initials":["J"],"affiliation":["Pôle des Maladies Infectieuses et Emergentes, Institut Louis Malardé, Papeete, Tahiti."]},{"fullName":["Bossin H"],"firstName":["Hervé"],"lastName":["Bossin"],"initials":["H"],"affiliation":["Pôle des Maladies Infectieuses et Emergentes, Institut Louis Malardé, Papeete, Tahiti."]},{"fullName":["Rama V"],"firstName":["Vineshwaran"],"lastName":["Rama"],"initials":["V"],"affiliation":["Ministry of Health, Suva, Fiji."]},{"fullName":["Faamoe A"],"firstName":["Akata"],"lastName":["Faamoe"],"initials":["A"],"affiliation":["Ministry of Health, Nuku'alofa, Kingdom of Tonga."]},{"fullName":["Kilama S"],"firstName":["Sosiasi"],"lastName":["Kilama"],"initials":["S"],"affiliation":["URE-Entomologie Médicale, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."]},{"fullName":["Teurlai M"],"firstName":["Magali"],"lastName":["Teurlai"],"initials":["M"],"affiliation":["URE-Epidémiologie des Maladies Infectieuses, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."]},{"fullName":["Mathieu-Daudé F"],"firstName":["Françoise"],"lastName":["Mathieu-Daudé"],"initials":["F"],"affiliation":["UMR IRD 224-CNRS 5290-UM1-UM2, MIVEGEC, Institut de Recherche pour le Développement, Noumea, New Caledonia."]},{"fullName":["Dupont-Rouzeyrol M"],"firstName":["Myrielle"],"lastName":["Dupont-Rouzeyrol"],"initials":["M"],"affiliation":["URE-Dengue et autres Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."]}]}],"journalInfo":[{"issue":["1"],"volume":["10"],"journalIssueId":["2358496"],"dateOfPublication":["2016 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004374"],"abstractText":["The Pacific region is an area unique in the world, composed of thousands of islands with differing climates and environments. The spreading and establishment of the mosquito Aedes aegypti in these islands might be linked to human migration. Ae. aegypti is the major vector of arboviruses (dengue, chikungunya and Zika viruses) in the region. The intense circulation of these viruses in the Pacific during the last decade led to an increase of vector control measures by local health authorities. The aim of this study is to analyze the genetic relationships among Ae. aegypti populations in this region.We studied the genetic variability and population genetics of 270 Ae. aegypti, sampled from 9 locations in New Caledonia, Fiji, Tonga and French Polynesia by analyzing nine microsatellites and two mitochondrial DNA regions (CO1 and ND4). Microsatellite markers revealed heterogeneity in the genetic structure between the western, central and eastern Pacific island countries. The microsatellite markers indicate a statistically moderate differentiation (FST = 0.136; P < = 0.001) in relation to island isolation. A high degree of mixed ancestry can be observed in the most important towns (e.g. Noumea, Suva and Papeete) compared with the most isolated islands (e.g. Ouvea and Vaitahu). Phylogenetic analysis indicated that most of samples are related to Asian and American specimens.Our results suggest a link between human migrations in the Pacific region and the origin of Ae. aegypti populations. The genetic pattern observed might be linked to the island isolation and to the different environmental conditions or ecosystems."],"affiliation":["URE-Dengue et autres Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International Institut Pasteur, Noumea, New Caledonia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Arboviruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA"]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Mitochondrial"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["Y"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Microsatellite Repeats"]},{"majorTopic_YN":["N"],"descriptorName":["Pacific Islands"]},{"majorTopic_YN":["Y"],"descriptorName":["Genetic Variation"]}]}],"chemicalList":[{"chemical":[{"name":["DNA"],"registryNumber":["9007-49-2"]},{"name":["DNA, Mitochondrial"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4723151?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4723151"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004374"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-05-02"],"dateOfCreation":["2016-01-23"],"dateOfRevision":["2016-01-31"],"electronicPublicationDate":["2016-01-22"],"firstPublicationDate":["2016-01-22"],"cprojectID":"PMC4723151"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnronJ9hGurOzZIa9","_score":1,"_source":{"id":["27352279"],"source":["MED"],"pmid":["27352279"],"pmcid":["PMC4931337"],"doi":["10.1038/ncomms12204"],"title":["A rhesus macaque model of Asian-lineage Zika virus infection."],"authorString":["Dudley DM, Aliota MT, Mohr EL, Weiler AM, Lehrer-Brey G, Weisgrau KL, Mohns MS, Breitbach ME, Rasheed MN, Newman CM, Gellerup DD, Moncla LH, Post J, Schultz-Darken N, Schotzko ML, Hayes JM, Eudailey JA, Moody MA, Permar SR, O'Connor SL, Rakasz EG, Simmons HA, Capuano S, Golos TG, Osorio JE, Friedrich TC, O'Connor DH."],"authorList":[{"author":[{"fullName":["Dudley DM"],"firstName":["Dawn M"],"lastName":["Dudley"],"initials":["DM"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Aliota MT"],"firstName":["Matthew T"],"lastName":["Aliota"],"initials":["MT"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA."]},{"fullName":["Mohr EL"],"firstName":["Emma L"],"lastName":["Mohr"],"initials":["EL"],"affiliation":["Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Weiler AM"],"firstName":["Andrea M"],"lastName":["Weiler"],"initials":["AM"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Lehrer-Brey G"],"firstName":["Gabrielle"],"lastName":["Lehrer-Brey"],"initials":["G"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Weisgrau KL"],"firstName":["Kim L"],"lastName":["Weisgrau"],"initials":["KL"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Mohns MS"],"firstName":["Mariel S"],"lastName":["Mohns"],"initials":["MS"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Breitbach ME"],"firstName":["Meghan E"],"lastName":["Breitbach"],"initials":["ME"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Rasheed MN"],"firstName":["Mustafa N"],"lastName":["Rasheed"],"initials":["MN"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Newman CM"],"firstName":["Christina M"],"lastName":["Newman"],"initials":["CM"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Gellerup DD"],"firstName":["Dane D"],"lastName":["Gellerup"],"initials":["DD"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Moncla LH"],"firstName":["Louise H"],"lastName":["Moncla"],"initials":["LH"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA."]},{"fullName":["Post J"],"firstName":["Jennifer"],"lastName":["Post"],"initials":["J"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Schultz-Darken N"],"firstName":["Nancy"],"lastName":["Schultz-Darken"],"initials":["N"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Schotzko ML"],"firstName":["Michele L"],"lastName":["Schotzko"],"initials":["ML"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Hayes JM"],"firstName":["Jennifer M"],"lastName":["Hayes"],"initials":["JM"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Eudailey JA"],"firstName":["Josh A"],"lastName":["Eudailey"],"initials":["JA"],"affiliation":["Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA."]},{"fullName":["Moody MA"],"firstName":["M Anthony"],"lastName":["Moody"],"initials":["MA"],"affiliation":["Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA."]},{"fullName":["Permar SR"],"firstName":["Sallie R"],"lastName":["Permar"],"initials":["SR"],"affiliation":["Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA."]},{"fullName":["O'Connor SL"],"firstName":["Shelby L"],"lastName":["O'Connor"],"initials":["SL"],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."]},{"fullName":["Rakasz EG"],"firstName":["Eva G"],"lastName":["Rakasz"],"initials":["EG"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Simmons HA"],"firstName":["Heather A"],"lastName":["Simmons"],"initials":["HA"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Capuano S"],"firstName":["Saverio"],"lastName":["Capuano"],"initials":["S"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["Golos TG"],"firstName":["Thaddeus G"],"lastName":["Golos"],"initials":["TG"],"affiliation":["Department of Comparative Biosciences and Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA."]},{"fullName":["Osorio JE"],"firstName":["Jorge E"],"lastName":["Osorio"],"initials":["JE"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA."]},{"fullName":["Friedrich TC"],"firstName":["Thomas C"],"lastName":["Friedrich"],"initials":["TC"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]},{"fullName":["O'Connor DH"],"firstName":["David H"],"lastName":["O'Connor"],"initials":["DH"],"affiliation":["Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2358575"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Nature communications"],"ISOAbbreviation":["Nat Commun"],"medlineAbbreviation":["Nat Commun"],"NLMid":["101528555"],"ESSN":["2041-1723"]}]}],"pubYear":["2016"],"pageInfo":["12204"],"abstractText":["Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy."],"affiliation":["Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["P51 OD011106"],"agency":["NIH HHS"],"acronym":["OD"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4931337?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4931337"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/ncomms12204"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-06-29"],"dateOfRevision":["2016-07-15"],"electronicPublicationDate":["2016-06-28"],"firstPublicationDate":["2016-06-28"],"cprojectID":"PMC4931337"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrpThOUS2orL1IRW","_score":1,"_source":{"id":["27429487"],"source":["MED"],"pmid":["27429487"],"pmcid":["PMC4933147"],"doi":["10.2471/blt.16.030716"],"title":["Clean energy benefits the climate, the economy and our health."],"journalInfo":[{"issue":["7"],"volume":["94"],"journalIssueId":["2430669"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["489-490"],"abstractText":["Jeffrey D Sachs tells Fiona Fleck why investing in renewable energy is good for our health, but why poor countries need more time to make the switch."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933147?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933147"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.030716"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4933147"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrp-J9hGurOzZIbJ","_score":1,"_source":{"id":["27355468"],"source":["MED"],"pmid":["27355468"],"pmcid":["PMC4936892"],"doi":["10.1590/s1518-8787.2016050006791"],"title":["Emerging arboviruses and public health challenges in Brazil."],"authorString":["Lima-Camara TN."],"authorList":[{"author":[{"fullName":["Lima-Camara TN"],"firstName":["Tamara Nunes"],"lastName":["Lima-Camara"],"initials":["TN"],"affiliation":["Departamento de Epidemiologia. Faculdade de Saúde Pública. Universidade de São Paulo. São Paulo, SP, Brasil."]}]}],"journalInfo":[{"volume":["50"],"journalIssueId":["2420128"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Revista de saude publica"],"ISOAbbreviation":["Rev Saude Publica"],"medlineAbbreviation":["Rev Saude Publica"],"NLMid":["0135043"],"ISSN":["0034-8910"],"ESSN":["1518-8787"]}]}],"pubYear":["2016"],"abstractText":["Environmental modification by anthropogenic actions, disordered urban growth, globalization of international exchange and climate change are some factors that help the emergence and dissemination of human infectious diseases transmitted by vectors. This review discusses the recent entry of three arboviruses in Brazil: Chikungunya, West Nile, and Zika virus, focusing on the challenges for the Country's public health. The Brazilian population is exposed to infections caused by these three arboviruses widely distributed on the national territory and associated with humans. Without effective vaccine and specific treatment, the maintainance and integration of a continuos entomological and epidemiological surveillance are important so we can set methods to control and prevent these arboviruses in the Country. RESUMO A modificação do ambiente por ações antrópicas, o crescimento urbano desordenado, o processo de globalização do intercâmbio internacional e as mudanças climáticas são alguns fatores que vêm facilitando a emergência e disseminação de doenças infecciosas humanas transmitidas por vetores. Este comentário aborda a recente entrada de três arbovírus no Brasil, Chikungunya (CHIKV), West Nile (WNV) e Zika (ZIKV), com enfoque nos desafios para a Saúde Pública do País. Transmitidos por mosquitos vetores amplamente distribuídos no território nacional e associados ao homem, a população brasileira encontra-se exposta à infecção por esses três arbovírus. Na ausência de vacina eficaz e tratamento específico, são importantes a manutenção e integração de uma vigilância entomológica e epidemiológica contínua, a fim de direcionarmos métodos de controle e prevenção contra essas arboviroses no País."],"affiliation":["Departamento de Epidemiologia. Faculdade de Saúde Pública. Universidade de São Paulo. São Paulo, SP, Brasil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4936892?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4936892"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/S1518-8787.2016050006791"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-30"],"dateOfRevision":["2016-07-19"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4936892"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrqOhOUS2orL1IRa","_score":1,"_source":{"id":["27404496"],"source":["MED"],"pmid":["27404496"],"pmcid":["PMC4942056"],"doi":["10.1371/journal.pone.0159240"],"title":["Biodiversity and Temporal Distribution of Immature Culicidae in the Atlantic Forest, Rio de Janeiro State, Brazil."],"authorString":["Alencar J, de Mello CF, Serra-Freire NM, Guimarães AÉ, Gil-Santana HR, Gleiser RM."],"authorList":[{"author":[{"fullName":["Alencar J"],"firstName":["Jeronimo"],"lastName":["Alencar"],"initials":["J"],"authorId":[{"_":"0000-0001-7863-2698","$":{"type":"ORCID"}}],"affiliation":["Diptera Laboratory, Oswaldo Cruz Institute (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["de Mello CF"],"firstName":["Cecília Ferreira"],"lastName":["de Mello"],"initials":["CF"],"affiliation":["Postgraduate Program on Animal Biology, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil."]},{"fullName":["Serra-Freire NM"],"firstName":["Nicolau Maués"],"lastName":["Serra-Freire"],"initials":["NM"],"affiliation":["National Reference Laboratory on Rickettsioses Vectors, Oswaldo Cruz Institute (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Guimarães AÉ"],"firstName":["Anthony Érico"],"lastName":["Guimarães"],"initials":["AÉ"],"affiliation":["Diptera Laboratory, Oswaldo Cruz Institute (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Gil-Santana HR"],"firstName":["Hélcio R"],"lastName":["Gil-Santana"],"initials":["HR"],"affiliation":["Diptera Laboratory, Oswaldo Cruz Institute (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Gleiser RM"],"firstName":["Raquel M"],"lastName":["Gleiser"],"initials":["RM"],"affiliation":["Centro de Relevamiento y Evaluación de Recursos Agrícolas y Naturales-Instituto Multidisciplinario de Biología Vegetal (Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Córdoba, CONICET-UNC), Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7863-2698","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["11"],"journalIssueId":["2424094"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0159240"],"abstractText":["To increase the knowledge of biodiversity and identify larval habitats used by immature mosquitoes in the Atlantic Forest, we conducted a study in areas with various stages of preservation within the Guapiaçu Ecological Reserve in Cachoeiras de Macacu, Rio de Janeiro state. The Culicidae fauna were sampled during February, April, June, August, October, and December 2012; February, March, April, May, June, August, October, and December 2013; and January and March 2014. Immature mosquitoes were collected with dippers and suction tubes (mouth aspirators). Over the sampling period, 2697 larvae of 56 species were collected, some of which are recognized vectors of human diseases. The larval mosquito community found in artificial habitats, temporary ground water, and phytotelmata differed between sites, except for the mosquito fauna in bromeliads, which were almost 80% similar. Species segregation was more evident between larval habitats than between sites. Culex usquatus was the dominant species and colonized the highest number of larval habitats. The artificial larval habitats found in REGUA were colonized by a great diversity of species and high abundance as well, thus human artifacts left by the public in the area that collect water may promote an increase in mosquito populations. Among the species collected, some are known or suspected vectors of pathogens to humans and/or veterinary relevance, and their medical relevance is discussed."],"affiliation":["Diptera Laboratory, Oswaldo Cruz Institute (Fiocruz), Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942056?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942056"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0159240"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-13"],"dateOfRevision":["2016-08-02"],"electronicPublicationDate":["2016-07-12"],"firstPublicationDate":["2016-07-12"],"cprojectID":"PMC4942056"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrrwhOUS2orL1IRf","_score":1,"_source":{"id":["27462882"],"source":["MED"],"pmid":["27462882"],"pmcid":["PMC4943367"],"doi":["10.1590/s1679-45082016ed3725"],"title":["Bioethical considerations at times of Zika virus."],"authorString":["Bueno MA, Grunspun H."],"authorList":[{"author":[{"fullName":["Bueno MA"],"firstName":["Marco Aurélio Scarpinella"],"lastName":["Bueno"],"initials":["MA"],"affiliation":["Hospital Israelita Albert Einstein, São Paulo, SP, Brazil."]},{"fullName":["Grunspun H"],"firstName":["Henrique"],"lastName":["Grunspun"],"initials":["H"],"affiliation":["Hospital Israelita Albert Einstein, São Paulo, SP, Brazil."]}]}],"journalInfo":[{"issue":["2"],"volume":["14"],"journalIssueId":["2434165"],"dateOfPublication":["2016 Apr-Jun"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Einstein (Sao Paulo, Brazil)"],"ISOAbbreviation":["Einstein (Sao Paulo)"],"medlineAbbreviation":["Einstein (Sao Paulo)"],"NLMid":["101281800"],"ISSN":["1679-4508"],"ESSN":["2317-6385"]}]}],"pubYear":["2016"],"pageInfo":["13-18"],"affiliation":["Hospital Israelita Albert Einstein, São Paulo, SP, Brazil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4943367?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4943367"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/S1679-45082016ED3725"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-28"],"dateOfRevision":["2016-08-12"],"firstPublicationDate":["2016-04-01"],"cprojectID":"PMC4943367"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrr_hOUS2orL1IRj","_score":1,"_source":{"id":["27306491"],"source":["MED"],"pmid":["27306491"],"pmcid":["PMC4955015"],"doi":["10.1021/acs.analchem.6b01632"],"title":["Instrument-Free Point-of-Care Molecular Detection of Zika Virus."],"authorString":["Song J, Mauk MG, Hackett BA, Cherry S, Bau HH, Liu C."],"authorList":[{"author":[{"fullName":["Song J"],"firstName":["Jinzhao"],"lastName":["Song"],"initials":["J"],"affiliation":["Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]},{"fullName":["Mauk MG"],"firstName":["Michael G"],"lastName":["Mauk"],"initials":["MG"],"affiliation":["Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]},{"fullName":["Hackett BA"],"firstName":["Brent A"],"lastName":["Hackett"],"initials":["BA"],"affiliation":["Department of Microbiology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]},{"fullName":["Cherry S"],"firstName":["Sara"],"lastName":["Cherry"],"initials":["S"],"affiliation":["Department of Microbiology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]},{"fullName":["Bau HH"],"firstName":["Haim H"],"lastName":["Bau"],"initials":["HH"],"affiliation":["Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]},{"fullName":["Liu C"],"firstName":["Changchun"],"lastName":["Liu"],"initials":["C"],"affiliation":["Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."]}]}],"journalInfo":[{"issue":["14"],"volume":["88"],"journalIssueId":["2431456"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Analytical chemistry"],"ISOAbbreviation":["Anal. 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We report on a highly sensitive reverse-transcription loop-mediated, isothermal amplification (RT-LAMP) assay for rapid detection of ZIKV and its implementation in a simple, easy-to-use, inexpensive, point-of-care (POC) disposable cassette that carries out all the unit operations from sample introduction to detection. For thermal control of the cassette, we use a chemically heated cup without a need for electrical power. Amplification products are detected with leuco crystal violet (LCV) dye by eye without a need for instrumentation. We demonstrated the utility of our POC diagnostic system by detecting ZIKV in oral samples with sensitivity of 5 plaque-forming units (PFU) in less than 40 min. Our system is particularly suitable for resource-poor settings, where centralized laboratory facilities, funds, and trained personnel are in short supply, and for use in doctors' offices, clinics, and at home."],"affiliation":["Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania , Philadelphia, Pennsylvania 19104, United States."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4955015?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4955015"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1021/acs.analchem.6b01632"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-24"],"electronicPublicationDate":["2016-06-29"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4955015"}}
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Promoting, scaling up data and information sharing between African researchers and international partners are of vital importance in accelerating open access at no cost. Free Open Access (FOA) health data and information acceptability, uptake tactics and sustainable mechanisms are urgently needed. These are critical in establishing real time and effective knowledge or evidence-based translation, proven and validated approaches, strategies and tools to strengthen and revamp health systems. As such, early and timely access to needed emerging public health information is meant to be instrumental and valuable for policy-makers, implementers, care providers, researchers, health-related institutions and stakeholders including populations when guiding health financing, and planning contextual programs."],"affiliation":["Department of Biochemistry and Pharmaceutical Sciences, Universite des Montagnes, Bangangté, Cameroon; Africa Disease Intelligence and Surveillance, Communication and Response (Africa DISCoR) Foundation, Yaoundé, Cameroon."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Information","Africa","Policy","Program","intervention","Evidence","Open Access","Free Access"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4955019?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4955019"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8662.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-10"],"dateOfCreation":["2016-08-10"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-05-09"],"firstPublicationDate":["2016-05-09"],"cprojectID":"PMC4955019"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtbhOUS2orL1IRn","_score":1,"_source":{"id":["18680646"],"source":["MED"],"pmid":["18680646"],"pmcid":["PMC2600394"],"doi":["10.3201/eid1408.080287"],"title":["Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007."],"authorString":["Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, Stanfield SM, Duffy MR."],"authorList":[{"author":[{"fullName":["Lanciotti RS"],"firstName":["Robert S"],"lastName":["Lanciotti"],"initials":["RS"],"affiliation":["Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA. rsl2@cdc.gov"]},{"fullName":["Kosoy OL"],"firstName":["Olga L"],"lastName":["Kosoy"],"initials":["OL"]},{"fullName":["Laven JJ"],"firstName":["Janeen J"],"lastName":["Laven"],"initials":["JJ"]},{"fullName":["Velez JO"],"firstName":["Jason O"],"lastName":["Velez"],"initials":["JO"]},{"fullName":["Lambert AJ"],"firstName":["Amy J"],"lastName":["Lambert"],"initials":["AJ"]},{"fullName":["Johnson AJ"],"firstName":["Alison J"],"lastName":["Johnson"],"initials":["AJ"]},{"fullName":["Stanfield SM"],"firstName":["Stephanie M"],"lastName":["Stanfield"],"initials":["SM"]},{"fullName":["Duffy MR"],"firstName":["Mark R"],"lastName":["Duffy"],"initials":["MR"]}]}],"journalInfo":[{"issue":["8"],"volume":["14"],"journalIssueId":["1546509"],"dateOfPublication":["2008 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2008"],"printPublicationDate":["2008-08-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2008"],"pageInfo":["1232-1239"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne flavivirus first isolated in Uganda from a sentinel monkey in 1947. Mosquito and sentinel animal surveillance studies have demonstrated that ZIKV is endemic to Africa and Southeast Asia, yet reported human cases are rare, with <10 cases reported in the literature. In June 2007, an epidemic of fever and rash associated with ZIKV was detected in Yap State, Federated States of Micronesia. We report the genetic and serologic properties of the ZIKV associated with this epidemic."],"affiliation":["Diagnostic and Reference Laboratory, Arbovirus Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado 80521, USA. rsl2@cdc.gov"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin G"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin M"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Serologic Tests"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Base Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Micronesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["Immunoglobulin G"],"registryNumber":["0"]},{"name":["Immunoglobulin M"],"registryNumber":["0"]},{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2600394?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2600394"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=18680646"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=18680646&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1408.080287"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["72"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2008-10-28"],"dateOfCreation":["2008-08-05"],"dateOfRevision":["2016-02-05"],"firstPublicationDate":["2008-08-01"],"cprojectID":"PMC2600394"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtjJ9hGurOzZIba","_score":1,"_source":{"id":["27457250"],"source":["MED"],"pmid":["27457250"],"pmcid":["PMC4960669"],"doi":["10.1186/s13071-016-1683-2"],"title":["The insect-specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes."],"authorString":["Hall-Mendelin S, McLean BJ, Bielefeldt-Ohmann H, Hobson-Peters J, Hall RA, van den Hurk AF."],"authorList":[{"author":[{"fullName":["Hall-Mendelin S"],"firstName":["Sonja"],"lastName":["Hall-Mendelin"],"initials":["S"],"affiliation":["Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, PO Box 594, Archerfield, 4108, QLD, Australia."]},{"fullName":["McLean BJ"],"firstName":["Breeanna J"],"lastName":["McLean"],"initials":["BJ"],"affiliation":["Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, 4072, QLD, Australia."]},{"fullName":["Bielefeldt-Ohmann H"],"firstName":["Helle"],"lastName":["Bielefeldt-Ohmann"],"initials":["H"],"affiliation":["School of Veterinary Science, The University of Queensland, Gatton Campus, Gatton, 4343, QLD, Australia."]},{"fullName":["Hobson-Peters J"],"firstName":["Jody"],"lastName":["Hobson-Peters"],"initials":["J"],"affiliation":["Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, 4072, QLD, Australia."]},{"fullName":["Hall RA"],"firstName":["Roy A"],"lastName":["Hall"],"initials":["RA"],"affiliation":["Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, 4072, QLD, Australia."]},{"fullName":["van den Hurk AF"],"firstName":["Andrew F"],"lastName":["van den Hurk"],"initials":["AF"],"affiliation":["Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, PO Box 594, Archerfield, 4108, QLD, Australia. andrew.vandenHurk@health.qld.gov.au."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["414"],"abstractText":["Insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. These viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. Following the isolation of the first Australian insect-specific flavivirus (ISF), Palm Creek virus (PCV), we investigated routes of infection and transmission of this virus in key Australian arbovirus vectors and its impact on replication and transmission of West Nile virus (WNV).Culex annulirostris, Aedes aegypti and Aedes vigilax were exposed to PCV, and infection, replication and transmission rates in individual mosquitoes determined. To test whether the virus could be transmitted vertically, progeny reared from eggs oviposited by PCV-inoculated Cx. annulirostris were analysed for the presence of PCV. To assess whether prior infection of mosquitoes with PCV could also suppress the transmission of pathogenic flaviviruses, PCV positive or negative Cx. annulirostris were subsequently exposed to WNV.No PCV-infected Cx. annulirostris were detected 16 days after feeding on an infectious blood meal. However, when intrathoracically inoculated with PCV, Cx. annulirostris infection rates were 100 %. Similar rates of infection were observed in Ae. aegypti (100 %) and Ae. vigilax (95 %). Notably, PCV was not detected in any saliva expectorates collected from any of these species. PCV was not detected in 1038 progeny reared from 59 PCV-infected Cx. annulirostris. After feeding on a blood meal containing 10(7) infectious units of WNV, significantly fewer PCV-infected Cx. annulirostris were infected or transmitted WNV compared to PCV negative mosquitoes. Immunohistochemistry revealed that PCV localized in the midgut epithelial cells, which are the first site of infection with WNV.Our results indicate that PCV cannot infect Cx. annulirostris via the oral route, nor be transmitted in saliva or vertically to progeny. We also provide further evidence that prior infection with insect-specific viruses can regulate the infection and transmission of pathogenic arboviruses."],"affiliation":["Public Health Virology, Forensic and Scientific Services, Department of Health, Queensland Government, PO Box 594, Archerfield, 4108, QLD, Australia."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes aegypti","West Nile virus","Insect-specific Flavivirus","Palm Creek Virus","Aedes Vigilax","Culex Annulirostris"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960669?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960669"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1683-2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-26"],"dateOfRevision":["2016-07-29"],"electronicPublicationDate":["2016-07-25"],"firstPublicationDate":["2016-07-25"],"cprojectID":"PMC4960669"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtz_8VqgcuJwvji","_score":1,"_source":{"id":["27458575"],"source":["MED"],"pmid":["27458575"],"pmcid":["PMC4935673"],"doi":["10.3389/fpubh.2016.00107"],"title":["The Precautionary Principle, Evidence-Based Medicine, and Decision Theory in Public Health Evaluation."],"authorString":["Fischer AJ, Ghelardi G."],"authorList":[{"author":[{"fullName":["Fischer AJ"],"firstName":["Alastair J"],"lastName":["Fischer"],"initials":["AJ"],"affiliation":["Office for Health Economics (OHE) , London , UK."]},{"fullName":["Ghelardi G"],"firstName":["Gemma"],"lastName":["Ghelardi"],"initials":["G"],"affiliation":["London School of Economics , London , UK."]}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2374588"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in public health"],"ISOAbbreviation":["Front Public Health"],"medlineAbbreviation":["Front Public Health"],"NLMid":["101616579"],"ESSN":["2296-2565"]}]}],"pubYear":["2016"],"pageInfo":["107"],"abstractText":["The precautionary principle (PP) has been used in the evaluation of the effectiveness and/or cost-effectiveness of interventions designed to prevent future harms in a range of activities, particularly in the area of the environment. Here, we provide details of circumstances under which the PP can be applied to the topic of harm reduction in Public Health. The definition of PP that we use says that the PP reverses the onus of proof of effectiveness between an intervention and its comparator when the intervention has been designed to reduce harm. We first describe the two frameworks used for health-care evaluation: evidence-based medicine (EBM) and decision theory (DT). EBM is usually used in treatment effectiveness evaluation, while either EBM or DT may be used in evaluating the effectiveness of the prevention of illness. For cost-effectiveness, DT is always used. The expectation in Public Health is that interventions employed to reduce harm will not actually increase harm, where \"harm\" in this context does not include opportunity cost. That implies that an intervention's effectiveness can often be assumed. Attention should therefore focus on its cost-effectiveness. This view is consistent with the conclusions of DT. It is also very close to the PP notion of reversing the onus of proof, but is not consistent with EBM as normally practiced, where the onus is on showing a new practice to be superior to usual practice with a sufficiently high degree of certainty. Under our definitions, we show that where DT and the PP differ in their evaluation is in cost-effectiveness, but only for decisions that involve potential catastrophic circumstances, where the nation-state will act as if it is risk-averse. In those cases, it is likely that the state will pay more, and possibly much more, than DT would allow, in an attempt to mitigate impending disaster. That is, the rules that until now have governed all cost-effectiveness analyses are shown not to apply to catastrophic situations, where the PP applies."],"affiliation":["Office for Health Economics (OHE) , London , UK."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Effectiveness","Cost-effectiveness","Decision Theory","evidence-based medicine","Precautionary Principle"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4935673?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4935673"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fpubh.2016.00107"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-26"],"dateOfCreation":["2016-07-26"],"dateOfRevision":["2016-07-29"],"electronicPublicationDate":["2016-07-07"],"firstPublicationDate":["2016-07-07"],"cprojectID":"PMC4935673"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrt3hOUS2orL1IRo","_score":1,"_source":{"id":["27435464"],"source":["MED"],"pmid":["27435464"],"pmcid":["PMC4958264"],"doi":["10.1128/mbio.01123-16"],"title":["Dengue Virus Envelope Dimer Epitope Monoclonal Antibodies Isolated from Dengue Patients Are Protective against Zika Virus."],"authorString":["Swanstrom JA, Plante JA, Plante KS, Young EF, McGowan E, Gallichotte EN, Widman DG, Heise MT, de Silva AM, Baric RS."],"authorList":[{"author":[{"fullName":["Swanstrom JA"],"firstName":["J A"],"lastName":["Swanstrom"],"initials":["JA"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Plante JA"],"firstName":["J A"],"lastName":["Plante"],"initials":["JA"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Plante KS"],"firstName":["K S"],"lastName":["Plante"],"initials":["KS"],"affiliation":["Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Young EF"],"firstName":["E F"],"lastName":["Young"],"initials":["EF"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["McGowan E"],"firstName":["E"],"lastName":["McGowan"],"initials":["E"],"affiliation":["Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Gallichotte EN"],"firstName":["E N"],"lastName":["Gallichotte"],"initials":["EN"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Widman DG"],"firstName":["D G"],"lastName":["Widman"],"initials":["DG"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Heise MT"],"firstName":["M T"],"lastName":["Heise"],"initials":["MT"],"affiliation":["Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["de Silva AM"],"firstName":["A M"],"lastName":["de Silva"],"initials":["AM"],"affiliation":["Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."]},{"fullName":["Baric RS"],"firstName":["R S"],"lastName":["Baric"],"initials":["RS"],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA rbaric@email.unc.edu."]}]}],"journalInfo":[{"issue":["4"],"volume":["7"],"journalIssueId":["2426379"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne flavivirus responsible for thousands of cases of severe fetal malformations and neurological disease since its introduction to Brazil in 2013. Antibodies to flaviviruses can be protective, resulting in lifelong immunity to reinfection by homologous virus. However, cross-reactive antibodies can complicate flavivirus diagnostics and promote more severe disease, as noted after serial dengue virus (DENV) infections. The endemic circulation of DENV in South America and elsewhere raises concerns that preexisting flavivirus immunity may modulate ZIKV disease and transmission potential. Here, we report on the ability of human monoclonal antibodies and immune sera derived from dengue patients to neutralize contemporary epidemic ZIKV strains. We demonstrate that a class of human monoclonal antibodies isolated from DENV patients neutralizes ZIKV in cell culture and is protective in a lethal murine model. We also tested a large panel of convalescent-phase immune sera from humans exposed to primary and repeat DENV infection. Although ZIKV is most closely related to DENV compared to other human-pathogenic flaviviruses, most DENV immune sera (73%) failed to neutralize ZIKV, while others had low (50% effective concentration [EC50], <1:100 serum dilution; 18%) or moderate to high (EC50, >1:100 serum dilution; 9%) levels of cross-neutralizing antibodies. Our results establish that ZIKV and DENV share epitopes that are targeted by neutralizing, protective human antibodies. The availability of potently neutralizing human monoclonal antibodies provides an immunotherapeutic approach to control life-threatening ZIKV infection and also points to the possibility of repurposing DENV vaccines to induce cross-protective immunity to ZIKV.ZIKV is an emerging arbovirus that has been associated with severe neurological birth defects and fetal loss in pregnant women and Guillain-Barré syndrome in adults. Currently, there is no vaccine or therapeutic for ZIKV. The identification of a class of antibodies (envelope dimer epitope 1 [EDE1]) that potently neutralizes ZIKV in addition to all four DENV serotypes points to a potential immunotherapeutic to combat ZIKV. This is especially salient given the precedent of antibody therapy to treat pregnant women infected with other viruses associated with microcephaly, such as cytomegalovirus and rubella virus. Furthermore, the identification of a functionally conserved epitope between ZIKV and DENV raises the possibility that a vaccine may be able to elicit neutralizing antibodies against both viruses."],"affiliation":["Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4958264?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4958264"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.01123-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-20"],"dateOfRevision":["2016-07-26"],"electronicPublicationDate":["2016-07-19"],"firstPublicationDate":["2016-07-19"],"cprojectID":"PMC4958264"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnruQJ9hGurOzZIbd","_score":1,"_source":{"id":["27459519"],"source":["MED"],"pmid":["27459519"],"pmcid":["PMC4961373"],"doi":["10.1371/journal.pntd.0004873"],"title":["Heat Sensitivity of wMel Wolbachia during Aedes aegypti Development."],"authorString":["Ulrich JN, Beier JC, Devine GJ, Hugo LE."],"authorList":[{"author":[{"fullName":["Ulrich JN"],"firstName":["Jill N"],"lastName":["Ulrich"],"initials":["JN"],"authorId":[{"_":"0000-0001-7704-072X","$":{"type":"ORCID"}}],"affiliation":["Leonard and Jayne Abess Center for Ecosystem Science and Policy, University of Miami, Coral Gables, Florida, United States of America."]},{"fullName":["Beier JC"],"firstName":["John C"],"lastName":["Beier"],"initials":["JC"],"affiliation":["Department of Public Health Sciences, University of Miami Miller School of Medicine, Miami, Florida, United States of America."]},{"fullName":["Devine GJ"],"firstName":["Gregor J"],"lastName":["Devine"],"initials":["GJ"],"affiliation":["QIMR Berghofer Medical Research Institute, Royal Brisbane Hospital, Brisbane, Australia."]},{"fullName":["Hugo LE"],"firstName":["Leon E"],"lastName":["Hugo"],"initials":["LE"],"affiliation":["QIMR Berghofer Medical Research Institute, Royal Brisbane Hospital, Brisbane, Australia."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7704-072X","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["10"],"journalIssueId":["2424122"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004873"],"abstractText":["The wMel strain of Wolbachia bacteria is known to prevent dengue and Zika virus transmission in the mosquito vector Aedes aegypti. Accordingly, the release of wMel-infected A. aegypti in endemic regions has been recommended by the World Health Organization as a potential strategy for controlling dengue and Zika outbreaks. However, the utility of this approach could be limited if high temperatures in the aquatic habitats where A. aegypti develop are detrimental to Wolbachia. We exposed wMel-infected A. aegypti eggs and larvae to fluctuating daily temperatures of 30-40°C for three, five, or seven days during their development. We found that Wolbachia levels in females emerging from heat treatments were significantly lower than in the controls that had developed at 20-30°C. Notably, seven days of high temperatures starting at the egg stage reduced Wolbachia levels in emerging females to less than 0.1% of the wMel control levels. However, after adult females returned to 20-30°C for 4-7 days, they experienced differing degrees of Wolbachia recovery. Our findings suggest that the spread of Wolbachia in wild A. aegypti populations and any consequent protection from dengue and Zika viruses might be limited in ecosystems that experience periods of extreme heat, but Wolbachia levels recover partially after temperatures return to normal."],"affiliation":["Leonard and Jayne Abess Center for Ecosystem Science and Policy, University of Miami, Coral Gables, Florida, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4961373?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4961373"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004873"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-27"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-07-26"],"firstPublicationDate":["2016-07-26"],"cprojectID":"PMC4961373"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrxSJ9hGurOzZIbp","_score":1,"_source":{"id":["27516632"],"source":["MED"],"pmid":["27516632"],"pmcid":["PMC4969993"],"doi":["10.2471/blt.16.020816"],"title":["Promising new tools to fight Aedes mosquitoes."],"journalInfo":[{"issue":["8"],"volume":["94"],"journalIssueId":["2440780"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["562-563"],"abstractText":["Two new tools for suppressing Aedes aegypti mosquito populations have been recommended for pilot testing. Carefully designed trials will be needed to see whether they actually reduce disease as well. Andréia Azevedo Soares reports."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969993?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969993"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.020816"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-12"],"dateOfRevision":["2016-08-17"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4969993"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwsJ9hGurOzZIbm","_score":1,"_source":{"id":["27479050"],"source":["MED"],"pmid":["27479050"],"pmcid":["PMC4968812"],"doi":["10.1371/journal.pone.0160196"],"title":["Using Wolbachia Releases to Estimate Aedes aegypti (Diptera: Culicidae) Population Size and Survival."],"authorString":["Garcia Gde A, Dos Santos LM, Villela DA, Maciel-de-Freitas R."],"authorList":[{"author":[{"fullName":["Garcia Gde A"],"firstName":["Gabriela de Azambuja"],"lastName":["Garcia"],"initials":["Gde A"],"affiliation":["Laboratório de Transmissão de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, Brazil."]},{"fullName":["Dos Santos LM"],"firstName":["Lilha Maria Barbosa"],"lastName":["Dos Santos"],"initials":["LM"],"affiliation":["Laboratório de Transmissão de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, Brazil."]},{"fullName":["Villela DA"],"firstName":["Daniel Antunes Maciel"],"lastName":["Villela"],"initials":["DA"],"affiliation":["Programa de Computação Científica, Fundação Oswaldo Cruz (PROCC), Rio de Janeiro, Brazil."]},{"fullName":["Maciel-de-Freitas R"],"firstName":["Rafael"],"lastName":["Maciel-de-Freitas"],"initials":["R"],"affiliation":["Laboratório de Transmissão de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, Brazil."]}]}],"journalInfo":[{"issue":["8"],"volume":["11"],"journalIssueId":["2435494"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0160196"],"abstractText":["Mosquitoes carrying the endosymbiont bacterium Wolbachia have been deployed in field trials as a biological control intervention due to Wolbachia effects on reducing transmission of arboviruses. We performed mark, release and recapture (MRR) experiments using Wolbachia as an internal marker with daily collections with BG-Traps during the first two weeks of releases in Rio de Janeiro, Brazil. The MRR design allowed us to investigate two critical parameters that determine whether Wolbachia would successful invade a field population: the probability of daily survival (PDS) of Wolbachia-infected Aedes aegypti females, and the wild population density during releases. Released females had a PDS of 0.82 and 0.89 in the first and second weeks, respectively, immediately after releases, which is well within the range of previous estimates of survivorship of wild mosquitoes in Rio de Janeiro. Abundance estimation of wild population varied up to 10-fold higher depending on the estimation method used (634-3565 females on the average-difference model to 6365-16188 females according to Lincoln-Petersen). Wolbachia-released mosquitoes were lower than the density estimation of their wild counterparts, irrespectively of the model used. Individually screening mosquitoes for the presence of Wolbachia reduced uncertainty on abundance estimations due to fluctuation in capturing per week. A successful invasion into local population requires Ae. aegypti fitness is unaffected by Wolbachia presence, but also reliable estimates on the population size of wild mosquitoes."],"affiliation":["Laboratório de Transmissão de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4968812?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4968812"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0160196"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-19"],"electronicPublicationDate":["2016-08-01"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4968812"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzBhOUS2orL1IR3","_score":1,"_source":{"id":["27500084"],"source":["MED"],"pmid":["27500084"],"pmcid":["PMC4971151"],"doi":["10.1016/j.idcr.2016.07.008"],"title":["Neonatal encephalitis due to Chikungunya vertical transmission: First report in Brazil."],"authorString":["Bandeira AC, Campos GS, Sardi SI, Rocha VF, Rocha GC."],"authorList":[{"author":[{"fullName":["Bandeira AC"],"firstName":["Antonio Carlos"],"lastName":["Bandeira"],"initials":["AC"],"affiliation":["Faculdade de Tecnologia e Ciências Medical School, Av. Luís Viana, 8812, Salvador - Bahia, Brazil."]},{"fullName":["Campos GS"],"firstName":["Gubio Soares"],"lastName":["Campos"],"initials":["GS"],"affiliation":["Federal University of Bahia, Virology Laboratory, Brazil."]},{"fullName":["Sardi SI"],"firstName":["Silvia Ines"],"lastName":["Sardi"],"initials":["SI"],"affiliation":["Federal University of Bahia, Virology Laboratory, Brazil."]},{"fullName":["Rocha VF"],"firstName":["Veronica França Diniz"],"lastName":["Rocha"],"initials":["VF"],"affiliation":["Hospital Geral Roberto Santos, Salvador, Brazil."]},{"fullName":["Rocha GC"],"firstName":["Guilherme Cesar Mendes"],"lastName":["Rocha"],"initials":["GC"],"affiliation":["Hospital Aliança, Salvador, Brazil."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2422930"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["IDCases"],"ISOAbbreviation":["IDCases"],"medlineAbbreviation":["IDCases"],"NLMid":["101634540"],"ESSN":["2214-2509"]}]}],"pubYear":["2016"],"pageInfo":["57-59"],"abstractText":["To report the first case of Chikungunya encephalitis acquired in the perinatal period during the current outbreak in Brazil.Case report.A male neonate with 3500 g developed macular erythematous rash, hypoactivity and fever progressing to generalized seizures. His mother had experienced a disseminated rash and fever before delivery. EEG showed diffuse slowing and cranial NMR was suggestive of encephalitis. Rt-PCR for Chikungunya virus (CHIKV) was positive in cerebrospinal fluid (CSF), blood, urine and saliva. The newborn was discharged home with neurological improvement.We report the first case of a perinatal CHIKV infection associated with a rapidly evolving encephalitis and an extensive dissemination of the virus as documented by positive rt-PCR results in CSF, blood, urine and saliva in the present outbreak in Brazil. In countries experiencing outbreaks of CHIKV infections, clinicians and neonatologists must be familiar with the possibility of the occurrence of neurologic complications and its possible consequences."],"affiliation":["Faculdade de Tecnologia e Ciências Medical School, Av. Luís Viana, 8812, Salvador - Bahia, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Neonatal Encephalitis","Neurological Syndromes","Chikungunya-associated Encephalitis","Chikungunya Neonatal","Vertical Transmission."]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4971151?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4971151"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.idcr.2016.07.008"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-08"],"dateOfCreation":["2016-08-08"],"dateOfRevision":["2016-08-11"],"electronicPublicationDate":["2016-07-25"],"firstPublicationDate":["2016-07-25"],"cprojectID":"PMC4971151"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrx8_8VqgcuJwvjp","_score":1,"_source":{"id":["27412129"],"source":["MED"],"pmid":["27412129"],"pmcid":["PMC4942964"],"doi":["10.1186/s13071-016-1674-3"],"title":["New \"light\" for one-world approach toward safe and effective control of animal diseases and insect vectors from leishmaniac perspectives."],"authorString":["Chang KP, Kolli BK, New Light Group."],"authorList":[{"author":[{"fullName":["Chang KP"],"firstName":["Kwang Poo"],"lastName":["Chang"],"initials":["KP"],"authorId":[{"_":"0000-0002-1317-1218","$":{"type":"ORCID"}}],"affiliation":["Department of Microbiology/Immunology, Chicago Medical School/Rosalind Franklin University of Medicine and Science, 3333 Green Bay Rd, North Chicago, IL, 60064, USA. kwangpoo.chang@rosalindfranklin.edu."]},{"fullName":["Kolli BK"],"firstName":["Bala K"],"lastName":["Kolli"],"initials":["BK"],"affiliation":["Department of Microbiology/Immunology, Chicago Medical School/Rosalind Franklin University of Medicine and Science, 3333 Green Bay Rd, North Chicago, IL, 60064, USA."]},{"collectiveName":["New Light Group"]}]}],"investigatorList":[{"investigator":[{"fullName":["Batchu R"],"firstName":["RameshB"],"lastName":["Batchu"],"initials":["R"]},{"fullName":["Chen HW"],"firstName":["Hui-Wen"],"lastName":["Chen"],"initials":["HW"]},{"fullName":["Chow LC"],"firstName":["LarryMing C"],"lastName":["Chow"],"initials":["LC"]},{"fullName":["Elliott R"],"firstName":["Robert"],"lastName":["Elliott"],"initials":["R"]},{"fullName":["Head J"],"firstName":["JonathanF"],"lastName":["Head"],"initials":["J"]},{"fullName":["Fan CK"],"firstName":["Chia-Kwung"],"lastName":["Fan"],"initials":["CK"]},{"fullName":["Hung CH"],"firstName":["Chen-Hsiung"],"lastName":["Hung"],"initials":["CH"]},{"fullName":["Ji DD"],"firstName":["Dar-Der"],"lastName":["Ji"],"initials":["DD"]},{"fullName":["Lun ZR"],"firstName":["Zhao-Rong"],"lastName":["Lun"],"initials":["ZR"]},{"fullName":["Manna L"],"firstName":["Laura"],"lastName":["Manna"],"initials":["L"]},{"fullName":["Matsumoto Y"],"firstName":["Yoshitsugu"],"lastName":["Matsumoto"],"initials":["Y"]},{"fullName":["Ng DP"],"firstName":["DennisK P"],"lastName":["Ng"],"initials":["DP"]},{"fullName":["de Oliveira C"],"firstName":["CamilaI"],"lastName":["de Oliveira"],"initials":["C"]},{"fullName":["Melo S"],"firstName":["Sayonara"],"lastName":["Melo"],"initials":["S"]},{"fullName":["Ozbel Y"],"firstName":["Yusuf"],"lastName":["Ozbel"],"initials":["Y"]},{"fullName":["Özbilgin A"],"firstName":["Ahmet"],"lastName":["Özbilgin"],"initials":["A"]},{"fullName":["Reynolds J"],"firstName":["Joseph"],"lastName":["Reynolds"],"initials":["J"]},{"fullName":["Sanjoba C"],"firstName":["Chizu"],"lastName":["Sanjoba"],"initials":["C"]},{"fullName":["Shiao SH"],"firstName":["Shin-Hong"],"lastName":["Shiao"],"initials":["SH"]},{"fullName":["Shih NY"],"firstName":["Nang-Yao"],"lastName":["Shih"],"initials":["NY"]},{"fullName":["Tsai CW"],"firstName":["Chi-Wei"],"lastName":["Tsai"],"initials":["CW"]},{"fullName":["Vicente MG"],"firstName":["Mariada Graça H"],"lastName":["Vicente"],"initials":["MG"]},{"fullName":["Barré C"],"firstName":["CharlesH"],"lastName":["Barré"],"initials":["C"]},{"fullName":["Volf P"],"firstName":["Petr"],"lastName":["Volf"],"initials":["P"]},{"fullName":["Wu YL"],"firstName":["Yueh-Lung"],"lastName":["Wu"],"initials":["YL"]},{"fullName":["Yu CL"],"firstName":["Chao-Lan"],"lastName":["Yu"],"initials":["CL"]},{"fullName":["Zhou XN"],"firstName":["Xiao-Nong"],"lastName":["Zhou"],"initials":["XN"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1317-1218","$":{"type":"ORCID"}},{"_":"0000-0002-7749-7807","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["396"],"abstractText":["Light is known to excite photosensitizers (PS) to produce cytotoxic reactive oxygen species (ROS) in the presence of oxygen. This modality is attractive for designing control measures against animal diseases and pests. Many PS have a proven safety record. Also, the ROS cytotoxicity selects no resistant mutants, unlike other drugs and pesticides. Photodynamic therapy (PDT) refers to the use of PS as light activable tumoricides, microbicides and pesticides in medicine and agriculture.Here we describe \"photodynamic vaccination\" (PDV) that uses PDT-inactivation of parasites, i.e. Leishmania as whole-cell vaccines against leishmaniasis, and as a universal carrier to deliver transgenic add-on vaccines against other infectious and malignant diseases. The efficacy of Leishmania for vaccine delivery makes use of their inherent attributes to parasitize antigen (vaccine)-presenting cells. Inactivation of Leishmania by PDT provides safety for their use. This is accomplished in two different ways: (i) chemical engineering of PS to enhance their uptake, e.g. Si-phthalocyanines; and (ii) transgenic approach to render Leishmania inducible for porphyrinogenesis. Three different schemes of Leishmania-based PDV are presented diagrammatically to depict the cellular events resulting in cell-mediated immunity, as seen experimentally against leishmaniasis and Leishmania-delivered antigen in vitro and in vivo. Safety versus efficacy evaluations are under way for PDT-inactivated Leishmania, including those further processed to facilitate their storage and transport. Leishmania transfected to express cancer and viral vaccine candidates are being prepared accordingly for experimental trials.We have begun to examine PS-mediated photodynamic insecticides (PDI). Mosquito cells take up rose bengal/cyanosine, rendering them light-sensitive to undergo disintegration in vitro, thereby providing a cellular basis for the larvicidal activity seen by the same treatments. Ineffectiveness of phthalocyanines and porphyrins for PDI underscores its requirement for different PS. Differential uptake of PS by insect versus other cells to account for this difference is under study.The ongoing work is patterned after the one-world approach by enlisting the participation of experts in medicinal chemistry, cell/molecular biology, immunology, parasitology, entomology, cancer research, tropical medicine and veterinary medicine. The availability of multidisciplinary expertise is indispensable for implementation of the necessary studies to move the project toward product development."],"affiliation":["Department of Microbiology/Immunology, Chicago Medical School/Rosalind Franklin University of Medicine and Science, 3333 Green Bay Rd, North Chicago, IL, 60064, USA. kwangpoo.chang@rosalindfranklin.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Photosensitizers","Leishmania","Photodynamic therapy","Mosquito","Photodynamic Vaccination","Photodynamic Insecticide"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942964?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942964"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1674-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-14"],"dateOfRevision":["2016-07-16"],"electronicPublicationDate":["2016-07-13"],"firstPublicationDate":["2016-07-13"],"cprojectID":"PMC4942964"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXJ_8VqgcuJwviE","_score":1,"_source":{"id":["27124663"],"source":["MED"],"pmid":["27124663"],"pmcid":["PMC4849757"],"doi":["10.1371/journal.pntd.0004677"],"title":["The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti."],"authorString":["Aliota MT, Walker EC, Uribe Yepes A, Velez ID, Christensen BM, Osorio JE."],"authorList":[{"author":[{"fullName":["Aliota MT"],"firstName":["Matthew T"],"lastName":["Aliota"],"initials":["MT"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Walker EC"],"firstName":["Emma C"],"lastName":["Walker"],"initials":["EC"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Uribe Yepes A"],"firstName":["Alexander"],"lastName":["Uribe Yepes"],"initials":["A"],"affiliation":["Programa de Estudio y Control de Enfermedades Tropicales (PECET), Universidad de Antioquia, Medellin, Colombia."]},{"fullName":["Velez ID"],"firstName":["Ivan Dario"],"lastName":["Velez"],"initials":["ID"],"affiliation":["Programa de Estudio y Control de Enfermedades Tropicales (PECET), Universidad de Antioquia, Medellin, Colombia."]},{"fullName":["Christensen BM"],"firstName":["Bruce M"],"lastName":["Christensen"],"initials":["BM"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Osorio JE"],"firstName":["Jorge E"],"lastName":["Osorio"],"initials":["JE"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004677"],"abstractText":["New approaches to preventing chikungunya virus (CHIKV) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival. A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV) transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against CHIKV. Although this approach holds much promise for limiting virus transmission, at present our understanding of the ability of CHIKV to infect, disseminate, and be transmitted by wMel-infected Ae. aegypti currently being used at Wolbachia release sites is limited.Using Ae. aegypti infected with the wMel strain of Wolbachia that are being released in Medellin, Colombia, we report that these mosquitoes have reduced vector competence for CHIKV, even with extremely high viral titers in the bloodmeal. In addition, we examined the dynamics of CHIKV infection over the course of four to seven days post feeding. Wolbachia-infected mosquitoes remained non-infective over the duration of seven days, i.e., no infectious virus was detected in the saliva when exposed to bloodmeals of moderate viremia, but CHIKV-exposed, wild type mosquitoes did have viral loads in the saliva consistent with what has been reported elsewhere. Finally, the presence of wMel infection had no impact on the lifespan of mosquitoes as compared to wild type mosquitoes following CHIKV infection.These results could have an impact on vector control strategies in areas where Ae. aegypti are transmitting both DENV and CHIKV; i.e., they argue for further exploration, both in the laboratory and the field, on the feasibility of expanding this technology beyond DENV."],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R21 AI117413"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["#AI117413-01"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Saliva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Wolbachia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Survival Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["Y"],"descriptorName":["Antibiosis"]},{"majorTopic_YN":["N"],"descriptorName":["Disease Transmission, Infectious"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4849757?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4849757"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004677"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-29"],"dateOfRevision":["2016-05-20"],"electronicPublicationDate":["2016-04-28"],"firstPublicationDate":["2016-04-28"],"cprojectID":"PMC4849757"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXwJ9hGurOzZIaF","_score":1,"_source":{"id":["27186984"],"source":["MED"],"pmid":["27186984"],"pmcid":["PMC4871342"],"doi":["10.1371/journal.pntd.0004726"],"title":["Transmission Dynamics of Zika Virus in Island Populations: A Modelling Analysis of the 2013-14 French Polynesia Outbreak."],"authorString":["Kucharski AJ, Funk S, Eggo RM, Mallet HP, Edmunds WJ, Nilles EJ."],"authorList":[{"author":[{"fullName":["Kucharski AJ"],"firstName":["Adam J"],"lastName":["Kucharski"],"initials":["AJ"],"affiliation":["Centre for the Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Funk S"],"firstName":["Sebastian"],"lastName":["Funk"],"initials":["S"],"affiliation":["Centre for the Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Eggo RM"],"firstName":["Rosalind M"],"lastName":["Eggo"],"initials":["RM"],"authorId":[{"_":"0000-0002-0362-6717","$":{"type":"ORCID"}}],"affiliation":["Centre for the Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Mallet HP"],"firstName":["Henri-Pierre"],"lastName":["Mallet"],"initials":["HP"],"affiliation":["Bureau de Veille Sanitaire, Direction de la Santé, Polynésie française."]},{"fullName":["Edmunds WJ"],"firstName":["W John"],"lastName":["Edmunds"],"initials":["WJ"],"affiliation":["Centre for the Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Nilles EJ"],"firstName":["Eric J"],"lastName":["Nilles"],"initials":["EJ"],"authorId":[{"_":"0000-0001-7044-5257","$":{"type":"ORCID"}}],"affiliation":["World Health Organization, Suva, Fiji."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0362-6717","$":{"type":"ORCID"}},{"_":"0000-0001-7044-5257","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004726"],"abstractText":["Between October 2013 and April 2014, more than 30,000 cases of Zika virus (ZIKV) disease were estimated to have attended healthcare facilities in French Polynesia. ZIKV has also been reported in Africa and Asia, and in 2015 the virus spread to South America and the Caribbean. Infection with ZIKV has been associated with neurological complications including Guillain-Barré Syndrome (GBS) and microcephaly, which led the World Health Organization to declare a Public Health Emergency of International Concern in February 2015. To better understand the transmission dynamics of ZIKV, we used a mathematical model to examine the 2013-14 outbreak on the six major archipelagos of French Polynesia. Our median estimates for the basic reproduction number ranged from 2.6-4.8, with an estimated 11.5% (95% CI: 7.32-17.9%) of total infections reported. As a result, we estimated that 94% (95% CI: 91-97%) of the total population of the six archipelagos were infected during the outbreak. Based on the demography of French Polynesia, our results imply that if ZIKV infection provides complete protection against future infection, it would take 12-20 years before there are a sufficient number of susceptible individuals for ZIKV to re-emerge, which is on the same timescale as the circulation of dengue virus serotypes in the region. Our analysis suggests that ZIKV may exhibit similar dynamics to dengue virus in island populations, with transmission characterized by large, sporadic outbreaks with a high proportion of asymptomatic or unreported cases."],"affiliation":["Centre for the Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871342?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871342"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004726"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-18"],"dateOfRevision":["2016-06-01"],"electronicPublicationDate":["2016-05-17"],"firstPublicationDate":["2016-05-17"],"cprojectID":"PMC4871342"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYdhOUS2orL1IQU","_score":1,"_source":{"id":["27178645"],"source":["MED"],"pmid":["27178645"],"pmcid":["PMC4867046"],"doi":["10.3402/gha.v9.31620"],"title":["Internet-based media coverage on dengue in Sri Lanka between 2007 and 2015."],"authorString":["Wilder-Smith A, Cohn E, Lloyd DC, Tozan Y, Brownstein JS."],"authorList":[{"author":[{"fullName":["Wilder-Smith A"],"firstName":["Annelies"],"lastName":["Wilder-Smith"],"initials":["A"],"affiliation":["Institute of Public Health, University of Heidelberg, Heidelberg, Germany; awilder-smith@ntu.edu.sg."]},{"fullName":["Cohn E"],"firstName":["Emily"],"lastName":["Cohn"],"initials":["E"],"affiliation":["Boston Children's Hospital, Boston, MA, USA."]},{"fullName":["Lloyd DC"],"firstName":["David C"],"lastName":["Lloyd"],"initials":["DC"],"affiliation":["Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore."]},{"fullName":["Tozan Y"],"firstName":["Yesim"],"lastName":["Tozan"],"initials":["Y"],"affiliation":["College of Global Public Health, New York University, New York, NY, USA."]},{"fullName":["Brownstein JS"],"firstName":["John S"],"lastName":["Brownstein"],"initials":["JS"],"affiliation":["Boston Children's Hospital, Boston, MA, USA."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2363164"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Global health action"],"ISOAbbreviation":["Glob Health Action"],"medlineAbbreviation":["Glob Health Action"],"NLMid":["101496665"],"ISSN":["1654-9716"],"ESSN":["1654-9880"]}]}],"pubYear":["2016"],"pageInfo":["31620"],"abstractText":["Internet-based media coverage to explore the extent of awareness of a disease and perceived severity of an outbreak at a national level can be used for early outbreak detection. Dengue has emerged as a major public health problem in Sri Lanka since 2009.To compare Internet references to dengue in Sri Lana with references to other diseases (malaria and influenza) in Sri Lanka and to compare Internet references to dengue in Sri Lanka with notified cases of dengue in Sri Lanka.We examined Internet-based news media articles on dengue queried from HealthMap for Sri Lanka, for the period January 2007 to November 2015. For comparative purposes, we compared hits on dengue with hits on influenza and malaria.There were 565 hits on dengue between 2007 and 2015, with a rapid rise in 2009 and followed by a rising trend ever since. These hits were highly correlated with the national epidemiological trend of dengue. The volume of digital media coverage of dengue was much higher than of influenza and malaria.Dengue in Sri Lanka is receiving increasing media attention. Our findings underpin previous claims that digital media reports reflect national epidemiological trends, both in annual trends and inter-annual seasonal variation, thus acting as proxy biosurveillance to provide early warning and situation awareness of emerging infectious diseases."],"affiliation":["Institute of Public Health, University of Heidelberg, Heidelberg, Germany; awilder-smith@ntu.edu.sg."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Malaria","Internet","Influenza","Media","Sri Lanka","Dengue","Healthmap","News Coverage","Awareness About Dengue","Emergence Of Dengue"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867046?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867046"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3402/gha.v9.31620"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-14"],"dateOfRevision":["2016-05-26"],"electronicPublicationDate":["2016-05-12"],"firstPublicationDate":["2016-05-12"],"cprojectID":"PMC4867046"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraSJ9hGurOzZIaN","_score":1,"_source":{"id":["27232603"],"source":["MED"],"pmid":["27232603"],"pmcid":["PMC4884096"],"doi":["10.1371/journal.pntd.0004764"],"title":["Correction: Spread of the Invasive Mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea Region Increases Risk of Chikungunya, Dengue, and Zika Outbreaks in Europe."],"authorString":["Akiner MM, Demirci B, Babuadze G, Robert V, Schaffner F."],"authorList":[{"author":[{"fullName":["Akiner MM"],"firstName":["Muhammet M"],"lastName":["Akiner"],"initials":["MM"]},{"fullName":["Demirci B"],"firstName":["Berna"],"lastName":["Demirci"],"initials":["B"]},{"fullName":["Babuadze G"],"firstName":["Giorgi"],"lastName":["Babuadze"],"initials":["G"]},{"fullName":["Robert V"],"firstName":["Vincent"],"lastName":["Robert"],"initials":["V"]},{"fullName":["Schaffner F"],"firstName":["Francis"],"lastName":["Schaffner"],"initials":["F"]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004764"],"abstractText":["[This corrects the article DOI: 10.1371/journal.pntd.0004664.]."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Published Erratum"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4884096?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4884096"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004764"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27115737"],"source":["MED"],"reference":["PLoS Negl Trop Dis. 2016 Apr 26;10(4):e0004664. doi: 10.1371/journal.pntd.0004664. eCollection 2016 Apr."],"type":["Erratum for"],"orderIn":["1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-27"],"dateOfRevision":["2016-05-27"],"electronicPublicationDate":["2016-05-27"],"firstPublicationDate":["2016-05-27"],"cprojectID":"PMC4884096"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraqhOUS2orL1IQf","_score":1,"_source":{"id":["27223693"],"source":["MED"],"pmid":["27223693"],"pmcid":["PMC4880295"],"doi":["10.1371/journal.pntd.0004621"],"title":["Temporal Dynamics and Spatial Patterns of Aedes aegypti Breeding Sites, in the Context of a Dengue Control Program in Tartagal (Salta Province, Argentina)."],"authorString":["Espinosa M, Weinberg D, Rotela CH, Polop F, Abril M, Scavuzzo CM."],"authorList":[{"author":[{"fullName":["Espinosa M"],"firstName":["Manuel"],"lastName":["Espinosa"],"initials":["M"],"affiliation":["Fundación Mundo Sano, Buenos Aires, Argentina."]},{"fullName":["Weinberg D"],"firstName":["Diego"],"lastName":["Weinberg"],"initials":["D"],"affiliation":["Fundación Mundo Sano, Buenos Aires, Argentina."]},{"fullName":["Rotela CH"],"firstName":["Camilo H"],"lastName":["Rotela"],"initials":["CH"],"affiliation":["Comisión Nacional de Actividades Espaciales, Falda del Carmen, Córdoba, Argentina."]},{"fullName":["Polop F"],"firstName":["Francisco"],"lastName":["Polop"],"initials":["F"],"affiliation":["Fundación Mundo Sano, Buenos Aires, Argentina."]},{"fullName":["Abril M"],"firstName":["Marcelo"],"lastName":["Abril"],"initials":["M"],"affiliation":["Fundación Mundo Sano, Buenos Aires, Argentina."]},{"fullName":["Scavuzzo CM"],"firstName":["Carlos Marcelo"],"lastName":["Scavuzzo"],"initials":["CM"],"affiliation":["Comisión Nacional de Actividades Espaciales, Falda del Carmen, Córdoba, Argentina."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004621"],"abstractText":["Since 2009, Fundación Mundo Sano has implemented an Aedes aegypti Surveillance and Control Program in Tartagal city (Salta Province, Argentina). The purpose of this study was to analyze temporal dynamics of Ae. aegypti breeding sites spatial distribution, during five years of samplings, and the effect of control actions over vector population dynamics.Seasonal entomological (larval) samplings were conducted in 17,815 fixed sites in Tartagal urban area between 2009 and 2014. Based on information of breeding sites abundance, from satellite remote sensing data (RS), and by the use of Geographic Information Systems (GIS), spatial analysis (hotspots and cluster analysis) and predictive model (MaxEnt) were performed. Spatial analysis showed a distribution pattern with the highest breeding densities registered in city outskirts. The model indicated that 75% of Ae. aegypti distribution is explained by 3 variables: bare soil coverage percentage (44.9%), urbanization coverage percentage(13.5%) and water distribution (11.6%).This results have called attention to the way entomological field data and information from geospatial origin (RS/GIS) are used to infer scenarios which could then be applied in epidemiological surveillance programs and in the determination of dengue control strategies. Predictive maps development constructed with Ae. aegypti systematic spatiotemporal data, in Tartagal city, would allow public health workers to identify and target high-risk areas with appropriate and timely control measures. These tools could help decision-makers to improve health system responses and preventive measures related to vector control."],"affiliation":["Fundación Mundo Sano, Buenos Aires, Argentina."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4880295?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4880295"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004621"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-26"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-25"],"firstPublicationDate":["2016-05-25"],"cprojectID":"PMC4880295"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraSJ9hGurOzZIaO","_score":1,"_source":{"id":["27080092"],"source":["MED"],"pmid":["27080092"],"pmcid":["PMC4882355"],"doi":["10.1007/s00381-016-3074-6"],"title":["Microcephaly and Zika virus: neonatal neuroradiological aspects."],"authorString":["Cavalheiro S, Lopez A, Serra S, Da Cunha A, da Costa MD, Moron A, Lederman HM."],"authorList":[{"author":[{"fullName":["Cavalheiro S"],"firstName":["Sergio"],"lastName":["Cavalheiro"],"initials":["S"],"affiliation":["Department of Neurosurgery, Federal University of Sao Paulo, Rua Botucatu, 591, conj 41, Sao Paulo, SP, 04023-062, Brazil. sergiocavalheironeuro@gmail.com."]},{"fullName":["Lopez A"],"firstName":["Amanda"],"lastName":["Lopez"],"initials":["A"],"affiliation":["Neurosurgery Service of Hospital da Restauração, Recife, Pernambuco, Brazil."]},{"fullName":["Serra S"],"firstName":["Suzana"],"lastName":["Serra"],"initials":["S"],"affiliation":["Neurosurgery Service of Hospital da Restauração, Recife, Pernambuco, Brazil."]},{"fullName":["Da Cunha A"],"firstName":["Arthur"],"lastName":["Da Cunha"],"initials":["A"],"affiliation":["Neurosurgery Service of Hospital da Restauração, Recife, Pernambuco, Brazil."]},{"fullName":["da Costa MD"],"firstName":["Marcos Devanir S"],"lastName":["da Costa"],"initials":["MD"],"affiliation":["Department of Neurosurgery, Federal University of Sao Paulo, Rua Botucatu, 591, conj 41, Sao Paulo, SP, 04023-062, Brazil."]},{"fullName":["Moron A"],"firstName":["Antonio"],"lastName":["Moron"],"initials":["A"],"affiliation":["Department of Obstetrics, Federal University of Sao Paulo, Sao Paulo, SP, Brazil."]},{"fullName":["Lederman HM"],"firstName":["Henrique M"],"lastName":["Lederman"],"initials":["HM"],"affiliation":["Department of Diagnostic Imaging, Federal University of Sao Paulo, Sao Paulo, SP, Brazil."]}]}],"journalInfo":[{"issue":["6"],"volume":["32"],"journalIssueId":["2412520"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery"],"ISOAbbreviation":["Childs Nerv Syst"],"medlineAbbreviation":["Childs Nerv Syst"],"NLMid":["8503227"],"ISSN":["0256-7040"],"ESSN":["1433-0350"]}]}],"pubYear":["2016"],"pageInfo":["1057-1060"],"abstractText":["The aim of this study is to describe some radiological features in the newborns with microcephaly caused by Zika virus infection during pregnancy.We radiologically analyzed 13 cases of newborns with microcephaly born to mothers who were infected by the Zika virus in the early stage of pregnancy.The most frequently observed radiological findings were microcephaly and decreased brain parenchymal volume associated with lissencephaly, ventriculomegaly secondary to the lack of brain tissue (not hypertensive), and coarse and anarchic calcifications mainly involving the subcortical cortical transition, and the basal ganglia.Although it cannot be concluded that there is a definitive pathognomonic radiographic pattern of microcephaly caused by Zika virus, gross calcifications and anarchic distribution involving the subcortical cortical transition and the basal ganglia, in association with lissencephaly and in the absence of hypertensive ventriculomegaly, are characteristic of this type of infection."],"affiliation":["Department of Neurosurgery, Federal University of Sao Paulo, Rua Botucatu, 591, conj 41, Sao Paulo, SP, 04023-062, Brazil. sergiocavalheironeuro@gmail.com."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes aegypti","Microcephaly","Lissencephaly","Ventriculomegaly","Intracranial Calcifications","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882355?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882355"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s00381-016-3074-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-27"],"dateOfRevision":["2016-06-22"],"electronicPublicationDate":["2016-04-14"],"firstPublicationDate":["2016-04-14"],"cprojectID":"PMC4882355"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrbIJ9hGurOzZIaV","_score":1,"_source":{"id":["27172988"],"source":["MED"],"pmid":["27172988"],"pmcid":["PMC4887331"],"doi":["10.1007/s13238-016-0275-4"],"title":["The crystal structure of Zika virus helicase: basis for antiviral drug design."],"authorString":["Tian H, Ji X, Yang X, Xie W, Yang K, Chen C, Wu C, Chi H, Mu Z, Wang Z, Yang H."],"authorList":[{"author":[{"fullName":["Tian H"],"firstName":["Hongliang"],"lastName":["Tian"],"initials":["H"],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China."]},{"fullName":["Ji X"],"firstName":["Xiaoyun"],"lastName":["Ji"],"initials":["X"],"affiliation":["The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210023, China."]},{"fullName":["Yang X"],"firstName":["Xiaoyun"],"lastName":["Yang"],"initials":["X"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Xie W"],"firstName":["Wei"],"lastName":["Xie"],"initials":["W"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Yang K"],"firstName":["Kailin"],"lastName":["Yang"],"initials":["K"],"affiliation":["Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, 44195, USA."]},{"fullName":["Chen C"],"firstName":["Cheng"],"lastName":["Chen"],"initials":["C"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Wu C"],"firstName":["Chen"],"lastName":["Wu"],"initials":["C"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Chi H"],"firstName":["Heng"],"lastName":["Chi"],"initials":["H"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Mu Z"],"firstName":["Zhongyu"],"lastName":["Mu"],"initials":["Z"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Wang Z"],"firstName":["Zefang"],"lastName":["Wang"],"initials":["Z"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Yang H"],"firstName":["Haitao"],"lastName":["Yang"],"initials":["H"],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. yanght@tju.edu.cn."]}]}],"journalInfo":[{"issue":["6"],"volume":["7"],"journalIssueId":["2413534"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Protein & cell"],"ISOAbbreviation":["Protein Cell"],"medlineAbbreviation":["Protein Cell"],"NLMid":["101532368"],"ISSN":["1674-800X"],"ESSN":["1674-8018"]}]}],"pubYear":["2016"],"pageInfo":["450-454"],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887331?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887331"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s13238-016-0275-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["PDB"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCreation":["2016-06-01"],"dateOfRevision":["2016-06-16"],"firstPublicationDate":["2016-05-12"],"cprojectID":"PMC4887331"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnre-hOUS2orL1IQt","_score":1,"_source":{"id":["27375618"],"source":["MED"],"pmid":["27375618"],"pmcid":["PMC4893500"],"doi":["10.3389/fimmu.2016.00218"],"title":["The Cellular Bases of Antibody Responses during Dengue Virus Infection."],"authorString":["Yam-Puc JC, Cedillo-Barrón L, Aguilar-Medina EM, Ramos-Payán R, Escobar-Gutiérrez A, Flores-Romo L."],"authorList":[{"author":[{"fullName":["Yam-Puc JC"],"firstName":["Juan Carlos"],"lastName":["Yam-Puc"],"initials":["JC"],"affiliation":["Department of Cell Biology, Center for Advanced Research, The National Polytechnic Institute, Cinvestav-IPN , Mexico City , Mexico."]},{"fullName":["Cedillo-Barrón L"],"firstName":["Leticia"],"lastName":["Cedillo-Barrón"],"initials":["L"],"affiliation":["Department of Molecular Biomedicine, Center for Advanced Research, The National Polytechnic Institute, Cinvestav-IPN , Mexico City , Mexico."]},{"fullName":["Aguilar-Medina EM"],"firstName":["Elsa Maribel"],"lastName":["Aguilar-Medina"],"initials":["EM"],"affiliation":["Faculty of Biological and Chemical Sciences, Autonomous University of Sinaloa (UAS) , Culiacan, Sinaloa , Mexico."]},{"fullName":["Ramos-Payán R"],"firstName":["Rosalío"],"lastName":["Ramos-Payán"],"initials":["R"],"affiliation":["Faculty of Biological and Chemical Sciences, Autonomous University of Sinaloa (UAS) , Culiacan, Sinaloa , Mexico."]},{"fullName":["Escobar-Gutiérrez A"],"firstName":["Alejandro"],"lastName":["Escobar-Gutiérrez"],"initials":["A"],"affiliation":["Department for Immunological Investigations, Institute for Epidemiological Diagnosis and Reference, Health Secretariat , Mexico City , Mexico."]},{"fullName":["Flores-Romo L"],"firstName":["Leopoldo"],"lastName":["Flores-Romo"],"initials":["L"],"affiliation":["Department of Cell Biology, Center for Advanced Research, The National Polytechnic Institute, Cinvestav-IPN , Mexico City , Mexico."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2377805"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in immunology"],"ISOAbbreviation":["Front Immunol"],"medlineAbbreviation":["Front Immunol"],"NLMid":["101560960"],"ESSN":["1664-3224"]}]}],"pubYear":["2016"],"pageInfo":["218"],"abstractText":["Dengue virus (DENV) is one of the most significant human viral pathogens transmitted by mosquitoes and can cause from an asymptomatic disease to mild undifferentiated fever, classical dengue, and severe dengue. Neutralizing memory antibody (Ab) responses are one of the most important mechanisms that counteract reinfections and are therefore the main aim of vaccination. However, it has also been proposed that in dengue, some of these class-switched (IgG) memory Abs might worsen the disease. Although these memory Abs derive from B cells by T-cell-dependent processes, we know rather little about the (acute, chronic, or memory) B cell responses and the complex cellular mechanisms generating these Abs during DENV infections. This review aims to provide an updated and comprehensive perspective of the B cell responses during DENV infection, starting since the very early events such as the cutaneous DENV entrance and the arrival into draining lymph nodes, to the putative B cell activation, proliferation, and germinal centers (GCs) formation (the source of affinity-matured class-switched memory Abs), till the outcome of GC reactions such as the generation of plasmablasts, Ab-secreting plasma cells, and memory B cells. We discuss topics very poorly explored such as the possibility of B cell infection by DENV or even activation-induced B cell death. The current information about the nature of the Ab responses to DENV is also illustrated."],"affiliation":["Department of Cell Biology, Center for Advanced Research, The National Polytechnic Institute, Cinvestav-IPN , Mexico City , Mexico."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Antibodies","Dengue virus","Plasma cells","Memory B Cells","In Vivo B Cell Responses"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4893500?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4893500"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fimmu.2016.00218"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-04"],"dateOfCreation":["2016-07-04"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-06"],"firstPublicationDate":["2016-06-06"],"cprojectID":"PMC4893500"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfmhOUS2orL1IQy","_score":1,"_source":{"id":["27278486"],"source":["MED"],"pmid":["27278486"],"pmcid":["PMC4898363"],"doi":["10.1186/s12985-016-0551-1"],"title":["Nucleotide composition of the Zika virus RNA genome and its codon usage."],"authorString":["van Hemert F, Berkhout B."],"authorList":[{"author":[{"fullName":["van Hemert F"],"firstName":["Formijn"],"lastName":["van Hemert"],"initials":["F"],"affiliation":["Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands."]},{"fullName":["Berkhout B"],"firstName":["Ben"],"lastName":["Berkhout"],"initials":["B"],"affiliation":["Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands. b.berkhout@amc.uva.nl."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2401989"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["95"],"abstractText":["RNA viruses have genomes with a distinct nucleotide composition and codon usage. We present the global characteristics of the RNA genome of Zika virus (ZIKV), an emerging pathogen within the Flavivirus genus. ZIKV was first isolated in 1947 in Uganda, caused a widespread epidemic in South and Central America and the Caribbean in 2015 and has recently been associated with microcephaly in newborns.The nearly 11 kb positive-stranded RNA genome of ZIKV was analyzed for its nucleotide composition, also in the context of the folded RNA molecule. Nucleotide trends were investigated along the genome length by skew analyses and we analyzed the codons used for translation of the ZIKV proteins.ZIKV RNA has a biased nucleotide composition in being purine-rich and pyrimidine-poor. This preference for purines is a general characteristic of the mosquito-borne and tick-borne flaviviruses. The virus-specific nucleotide bias is further enriched in the unpaired, single-stranded regions of the structured ZIKV RNA genome, thus further imposing this ZIKV-specific signature. The codons used for translation of the ZIKV proteins is also unusual, but we show that it is the underlying bias in nucleotide composition of the viral RNA that largely dictates these codon preferences.The ZIKV RNA genome has a biased nucleotide composition that dictates the codon usage of this flavivirus. We discuss the evolutionary scenarios and molecular mechanisms that may be responsible for these distinctive ZIKV RNA genome features."],"affiliation":["Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["RNA structure","Codon usage","Flavivirus","Nucleotide Composition","Zika Virus","Zikv","Nucleotide Bias","Viral Rna Genome","Purine-rich"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4898363?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4898363"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0551-1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["refseq"]}],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-12"],"electronicPublicationDate":["2016-06-08"],"firstPublicationDate":["2016-06-08"],"cprojectID":"PMC4898363"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhkJ9hGurOzZIar","_score":1,"_source":{"id":["27251981"],"source":["MED"],"pmid":["27251981"],"pmcid":["PMC4909981"],"doi":["10.2196/publichealth.5814"],"title":["Utilizing Nontraditional Data Sources for Near Real-Time Estimation of Transmission Dynamics During the 2015-2016 Colombian Zika Virus Disease Outbreak."],"authorString":["Majumder MS, Santillana M, Mekaru SR, McGinnis DP, Khan K, Brownstein JS."],"authorList":[{"author":[{"fullName":["Majumder MS"],"firstName":["Maimuna S"],"lastName":["Majumder"],"initials":["MS"],"authorId":[{"_":"0000-0002-3986-4303","$":{"type":"ORCID"}}],"affiliation":["Computational Epidemiology Group, Division of Emergency Medicine, Boston Children's Hospital, Boston, MA, United States. maimuna@mit.edu."]},{"fullName":["Santillana M"],"firstName":["Mauricio"],"lastName":["Santillana"],"initials":["M"],"authorId":[{"_":"0000-0002-4206-418X","$":{"type":"ORCID"}}]},{"fullName":["Mekaru SR"],"firstName":["Sumiko R"],"lastName":["Mekaru"],"initials":["SR"],"authorId":[{"_":"0000-0002-9782-3833","$":{"type":"ORCID"}}]},{"fullName":["McGinnis DP"],"firstName":["Denise P"],"lastName":["McGinnis"],"initials":["DP"],"authorId":[{"_":"0000-0002-5984-9338","$":{"type":"ORCID"}}]},{"fullName":["Khan K"],"firstName":["Kamran"],"lastName":["Khan"],"initials":["K"],"authorId":[{"_":"0000-0003-4367-960X","$":{"type":"ORCID"}}]},{"fullName":["Brownstein JS"],"firstName":["John S"],"lastName":["Brownstein"],"initials":["JS"],"authorId":[{"_":"0000-0001-8568-5317","$":{"type":"ORCID"}}]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3986-4303","$":{"type":"ORCID"}},{"_":"0000-0002-4206-418X","$":{"type":"ORCID"}},{"_":"0000-0002-9782-3833","$":{"type":"ORCID"}},{"_":"0000-0002-5984-9338","$":{"type":"ORCID"}},{"_":"0000-0003-4367-960X","$":{"type":"ORCID"}},{"_":"0000-0001-8568-5317","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["2"],"journalIssueId":["2413333"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["JMIR public health and surveillance"],"ISOAbbreviation":["JMIR Public Health Surveill"],"medlineAbbreviation":["JMIR Public Health Surveill"],"NLMid":["101669345"],"ESSN":["2369-2960"]}]}],"pubYear":["2016"],"pageInfo":["e30"],"abstractText":["Approximately 40 countries in Central and South America have experienced local vector-born transmission of Zika virus, resulting in nearly 300,000 total reported cases of Zika virus disease to date. Of the cases that have sought care thus far in the region, more than 70,000 have been reported out of Colombia.In this paper, we use nontraditional digital disease surveillance data via HealthMap and Google Trends to develop near real-time estimates for the basic (R) and observed (Robs) reproductive numbers associated with Zika virus disease in Colombia. We then validate our results against traditional health care-based disease surveillance data.Cumulative reported case counts of Zika virus disease in Colombia were acquired via the HealthMap digital disease surveillance system. Linear smoothing was conducted to adjust the shape of the HealthMap cumulative case curve using Google search data. Traditional surveillance data on Zika virus disease were obtained from weekly Instituto Nacional de Salud (INS) epidemiological bulletin publications. The Incidence Decay and Exponential Adjustment (IDEA) model was used to estimate R0 and Robs for both data sources.Using the digital (smoothed HealthMap) data, we estimated a mean R0 of 2.56 (range 1.42-3.83) and a mean Robs of 1.80 (range 1.42-2.30). The traditional (INS) data yielded a mean R0 of 4.82 (range 2.34-8.32) and a mean Robs of 2.34 (range 1.60-3.31).Although modeling using the traditional (INS) data yielded higher R estimates than the digital (smoothed HealthMap) data, modeled ranges for Robs were comparable across both data sources. As a result, the narrow range of possible case projections generated by the traditional (INS) data was largely encompassed by the wider range produced by the digital (smoothed HealthMap) data. Thus, in the absence of traditional surveillance data, digital surveillance data can yield similar estimates for key transmission parameters and should be utilized in other Zika virus-affected countries to assess outbreak dynamics in near real time."],"affiliation":["Computational Epidemiology Group, Division of Emergency Medicine, Boston Children's Hospital, Boston, MA, United States. maimuna@mit.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Reproductive number","Transmission Dynamics","Mathematical Modeling","Zika Virus Disease","Digital Disease Surveillance"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909981?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4909981"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2196/publichealth.5814"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-02"],"dateOfCreation":["2016-06-02"],"dateOfRevision":["2016-06-29"],"electronicPublicationDate":["2016-06-01"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4909981"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnriw_8VqgcuJwvit","_score":1,"_source":{"id":["27233238"],"source":["MED"],"pmid":["27233238"],"pmcid":["PMC4884387"],"doi":["10.1186/s40249-016-0148-3"],"title":["Outwitting dengue threat and epidemics resurgence in Asia-Pacific countries: strengthening integrated dengue surveillance, monitoring and response systems."],"authorString":["Tambo E, Chen JH, Zhou XN, Khater EI."],"authorList":[{"author":[{"fullName":["Tambo E"],"firstName":["Ernest"],"lastName":["Tambo"],"initials":["E"],"affiliation":["Public Health Pests Laboratory, Jeddah, Jeddah Governate, Saudi Arabia. tambo0711@gmail.com."]},{"fullName":["Chen JH"],"firstName":["Jun-Hu"],"lastName":["Chen"],"initials":["JH"],"affiliation":["WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, People's Republic of China."]},{"fullName":["Zhou XN"],"firstName":["Xiao-Nong"],"lastName":["Zhou"],"initials":["XN"],"affiliation":["WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, People's Republic of China. ipdzhouxn@sh163.net."]},{"fullName":["Khater EI"],"firstName":["Emad I M"],"lastName":["Khater"],"initials":["EI"],"affiliation":["Public Health Pests Laboratory, Jeddah, Jeddah Governate, Saudi Arabia."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2376921"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infectious diseases of poverty"],"ISOAbbreviation":["Infect Dis Poverty"],"medlineAbbreviation":["Infect Dis Poverty"],"NLMid":["101606645"],"ESSN":["2049-9957"]}]}],"pubYear":["2016"],"pageInfo":["56"],"abstractText":["Dengue is still a substantial vector-borne viral disease threat and burden of public health importance worldwide. This situation is complicated by dengue virus unprecedented resurgence and persistence of varied serotypes in endemic-prone areas, and man-made and natural activities consequences that promote vector emergence, transmission dynamics and spread across the Asia-Pacific region. There is an urgent need to strengthen operational and contextual surveillance-response research in improving early detection of active reservoir detection, novel drug in case management and quality evidence-based response including the deployment of dengue mass vaccination. Moreover, sustained mapping and watching of dengue risk factors or determinants, performance and outcome indicators of control or elimination programs effectiveness in defining minimum effective data towards community knowledge-based decision-making policy and effective response packages is imperative. Moreover, implementation of a robust, integrated dengue early warning surveillance, monitoring and response systems metrics is required for evidence-based, timely and cost-effective contextual mitigation strategies, and innovative interventions."],"affiliation":["Public Health Pests Laboratory, Jeddah, Jeddah Governate, Saudi Arabia. tambo0711@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"keywordList":[{"keyword":["China","Monitoring","Response","Resurgence","Surveillance","epidemics","Dengue","Asia-pacific"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4884387?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4884387"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40249-016-0148-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-28"],"dateOfRevision":["2016-05-31"],"electronicPublicationDate":["2016-05-27"],"firstPublicationDate":["2016-05-27"],"cprojectID":"PMC4884387"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlAhOUS2orL1IRB","_score":1,"_source":{"id":["27070847"],"source":["MED"],"pmid":["27070847"],"pmcid":["PMC4918188"],"doi":["10.3201/eid2207.160375"],"title":["Clinical Manifestations of Zika Virus Infection, Rio de Janeiro, Brazil, 2015."],"authorString":["Cerbino-Neto J, Mesquita EC, Souza TM, Parreira V, Wittlin BB, Durovni B, Lemos MC, Vizzoni A, Bispo de Filippis AM, Sampaio SA, Gonçalves Bde S, Bozza FA."],"authorList":[{"author":[{"fullName":["Cerbino-Neto J"],"firstName":["José"],"lastName":["Cerbino-Neto"],"initials":["J"]},{"fullName":["Mesquita EC"],"firstName":["Emersom Cicilini"],"lastName":["Mesquita"],"initials":["EC"]},{"fullName":["Souza TM"],"firstName":["Thiago Moreno L"],"lastName":["Souza"],"initials":["TM"]},{"fullName":["Parreira V"],"firstName":["Viviane"],"lastName":["Parreira"],"initials":["V"]},{"fullName":["Wittlin BB"],"firstName":["Bernardo Bastos"],"lastName":["Wittlin"],"initials":["BB"]},{"fullName":["Durovni B"],"firstName":["Betina"],"lastName":["Durovni"],"initials":["B"]},{"fullName":["Lemos MC"],"firstName":["Maria Cristina Ferreira"],"lastName":["Lemos"],"initials":["MC"]},{"fullName":["Vizzoni A"],"firstName":["Alexandre"],"lastName":["Vizzoni"],"initials":["A"]},{"fullName":["Bispo de Filippis AM"],"firstName":["Ana Maria"],"lastName":["Bispo de Filippis"],"initials":["AM"]},{"fullName":["Sampaio SA"],"firstName":["Simone Alves"],"lastName":["Sampaio"],"initials":["SA"]},{"fullName":["Gonçalves Bde S"],"firstName":["Bianca de Santis"],"lastName":["Gonçalves"],"initials":["Bde S"]},{"fullName":["Bozza FA"],"firstName":["Fernando A"],"lastName":["Bozza"],"initials":["FA"]}]}],"journalInfo":[{"issue":["7"],"volume":["22"],"journalIssueId":["2397213"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1318-1320"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Symptom","Brazil","RaSH","Surveillance","Fever","Headache","Conjunctivitis","Exanthema","Flavivirus","Arthralgia","Flaviviridae","Rio De Janeiro","Myalgia","Joint Swelling","Zika Virus","Zika Fever","Public Health Emergency Of International Concern","Pheic"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918188?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918188"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2207.160375"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"embargoDate":["2016-07-01"],"cprojectID":"PMC4918188"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmBJ9hGurOzZIa5","_score":1,"_source":{"id":["27294951"],"source":["MED"],"pmid":["27294951"],"pmcid":["PMC4926186"],"doi":["10.3390/v8060166"],"title":["Alphavirus Infection: Host Cell Shut-Off and Inhibition of Antiviral Responses."],"authorString":["Fros JJ, Pijlman GP."],"authorList":[{"author":[{"fullName":["Fros JJ"],"firstName":["Jelke J"],"lastName":["Fros"],"initials":["JJ"],"affiliation":["Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen 6700 AB, The Netherlands. jelke.fros@ndm.ox.ac.uk."]},{"fullName":["Pijlman GP"],"firstName":["Gorben P"],"lastName":["Pijlman"],"initials":["GP"],"affiliation":["Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen 6700 AB, The Netherlands. gorben.pijlman@wur.nl."]}]}],"journalInfo":[{"issue":["6"],"volume":["8"],"journalIssueId":["2412414"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2016"],"abstractText":["Alphaviruses cause debilitating disease in humans and animals and are transmitted by blood-feeding arthropods, typically mosquitoes. With a traditional focus on two models, Sindbis virus and Semliki Forest virus, alphavirus research has significantly intensified in the last decade partly due to the re-emergence and dramatic expansion of chikungunya virus in Asia, Europe, and the Americas. As a consequence, alphavirus-host interactions are now understood in much more molecular detail, and important novel mechanisms have been elucidated. It has become clear that alphaviruses not only cause a general host shut-off in infected vertebrate cells, but also specifically suppress different host antiviral pathways using their viral nonstructural proteins, nsP2 and nsP3. Here we review the current state of the art of alphavirus host cell shut-off of viral transcription and translation, and describe recent insights in viral subversion of interferon induction and signaling, the unfolded protein response, and stress granule assembly."],"affiliation":["Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen 6700 AB, The Netherlands. jelke.fros@ndm.ox.ac.uk."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Transcription","Translation","Interferon","Alphavirus","Unfolded protein response","Chikungunya","Sindbis","Stress Granules","Antiviral Response","Semliki Forest","Host Shut-off"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926186?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926186"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v8060166"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-14"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-11"],"firstPublicationDate":["2016-06-11"],"cprojectID":"PMC4926186"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmh_8VqgcuJwvi7","_score":1,"_source":{"id":["27267256"],"source":["MED"],"pmid":["27267256"],"pmcid":["PMC4897912"],"doi":["10.1186/s12879-016-1600-5"],"title":["Global research trends of Middle East respiratory syndrome coronavirus: a bibliometric analysis."],"authorString":["Zyoud SH."],"authorList":[{"author":[{"fullName":["Zyoud SH"],"firstName":["Sa'ed H"],"lastName":["Zyoud"],"initials":["SH"],"affiliation":["Poison Control and Drug Information Center (PCDIC), Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, 44839, Palestine. saedzyoud@yahoo.com."]}]}],"journalInfo":[{"volume":["16"],"journalIssueId":["2401698"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC infectious diseases"],"ISOAbbreviation":["BMC Infect. Dis."],"medlineAbbreviation":["BMC Infect Dis"],"NLMid":["100968551"],"ESSN":["1471-2334"]}]}],"pubYear":["2016"],"pageInfo":["255"],"abstractText":["Middle East respiratory syndrome coronavirus (MERS-CoV) is a virus that causes severe viral pneumonia in humans, known to have a high mortality rate and a similarity in clinical symptoms with severe acute respiratory syndrome coronavirus. It was first isolated in Kingdom of Saudi Arabia (KSA) in 2012 and after that, MERS-CoV exhibited outbreaks in several regions of the world. This study aimed to assess the characteristics of publications involving MERS-CoV at global level by using a bibliometric analysis.Scopus database was searched on March 4, 2016 for MERS-CoV publications published between 2012 and 2015. It was performed on the same day in order to avoid the possible bias came from update on the database because the metrics are changing over time. All publication types were considered; however publications as errata were excluded. Analysis parameters include year of publication, publication type, patterns of international collaboration, research institutions, journals, impact factor, h-index, language, and times cited.A total of 883 MERS-CoV research publications were published across the world. The MERS-CoV-associated publications were originated from 92 countries/territories, indicating the international spread of MERS-CoV research. The USA was the largest contributor, with 319 articles published over 4 years, followed by KSA (113 articles). The total number of citations for these publications has already achieved 8,015, with an average of 9.01 citations per each publication. The h-index for MERS-CoV-associated publications was 48. The USA also have the highest h-index (32), followed by KSA (26) and UK (22). Netherland produced the greatest proportion of publications with international research collaboration (72.7 %) followed by the UK (71 %) and Germany (69.1 %) out of the total number of publications for each country.There is a rapid increase in research activities related to MERS-CoV from 2012 to 2015. This study demonstrates that the MERS-CoV related literature has grown to be more extensive and global over the past 4 years. The bulk of publications in the field of MERS-CoV research are published by high-income countries such as the USA. Furthermore, the USA, the UK and KSA may have higher quality of articles according to the value of h-index."],"affiliation":["Poison Control and Drug Information Center (PCDIC), Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, 44839, Palestine. saedzyoud@yahoo.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Coronavirus","Scopus","Bibliometric","Mers-cov","Middle East Respiratory Syndrome Coronavirus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4897912?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4897912"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12879-016-1600-5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-12"],"electronicPublicationDate":["2016-06-07"],"firstPublicationDate":["2016-06-07"],"cprojectID":"PMC4897912"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmKhOUS2orL1IRI","_score":1,"_source":{"id":["27443350"],"source":["MED"],"pmid":["27443350"],"pmcid":["PMC4923202"],"doi":["10.3389/fmicb.2016.01028"],"title":["Commentary: Zika Virus: the Latest Newcomer."],"authorString":["Craig AT, Paterson BJ, Durrheim DN."],"authorList":[{"author":[{"fullName":["Craig AT"],"firstName":["Adam T"],"lastName":["Craig"],"initials":["AT"],"affiliation":["School of Medicine and Public Health, University of Newcastle Callaghan, NSW, Australia."]},{"fullName":["Paterson BJ"],"firstName":["Beverley J"],"lastName":["Paterson"],"initials":["BJ"],"affiliation":["School of Medicine and Public Health, University of Newcastle Callaghan, NSW, Australia."]},{"fullName":["Durrheim DN"],"firstName":["David N"],"lastName":["Durrheim"],"initials":["DN"],"affiliation":["School of Medicine and Public Health, University of Newcastle Callaghan, NSW, Australia."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2369092"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2016"],"pageInfo":["1028"],"affiliation":["School of Medicine and Public Health, University of Newcastle Callaghan, NSW, Australia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Comment","Journal Article"]}],"keywordList":[{"keyword":["Surveillance","epidemiology","Outbreaks","Communicable Diseases","Disease Surveillance","Emerging","Zika Virus (Zikv)","Communicable Disease Epidemiology"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4923202?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4923202"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2016.01028"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27148186"],"source":["MED"],"reference":["Front Microbiol. 2016;7:496"],"type":["Comment on"],"orderIn":["1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-22"],"dateOfCreation":["2016-07-22"],"dateOfRevision":["2016-07-25"],"electronicPublicationDate":["2016-06-28"],"firstPublicationDate":["2016-06-28"],"cprojectID":"PMC4923202"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrH8_8VqgcuJwvhc","_score":1,"_source":{"id":["27006779"],"source":["MED"],"pmid":["27006779"],"pmcid":["PMC4786754"],"doi":["10.1016/j.nmni.2016.02.003"],"title":["Simultaneous outbreaks of dengue, chikungunya and Zika virus infections: diagnosis challenge in a returning traveller with nonspecific febrile illness."],"authorString":["Moulin E, Selby K, Cherpillod P, Kaiser L, Boillat-Blanco N."],"authorList":[{"author":[{"fullName":["Moulin E"],"firstName":["E"],"lastName":["Moulin"],"initials":["E"],"affiliation":["Infectious Diseases Service, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland."]},{"fullName":["Selby K"],"firstName":["K"],"lastName":["Selby"],"initials":["K"],"affiliation":["Department of Ambulatory Care and Community Medicine, Lausanne, Switzerland."]},{"fullName":["Cherpillod P"],"firstName":["P"],"lastName":["Cherpillod"],"initials":["P"],"affiliation":["Swiss Reference Centre for Emerging Viral Diseases, Laboratory of Virology, University Hospital of Geneva, Geneva, Switzerland."]},{"fullName":["Kaiser L"],"firstName":["L"],"lastName":["Kaiser"],"initials":["L"],"affiliation":["Swiss Reference Centre for Emerging Viral Diseases, Laboratory of Virology, University Hospital of Geneva, Geneva, Switzerland; Infectious Diseases Service, University Hospital of Geneva, Geneva, Switzerland."]},{"fullName":["Boillat-Blanco N"],"firstName":["N"],"lastName":["Boillat-Blanco"],"initials":["N"],"affiliation":["Infectious Diseases Service, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland; Department of Ambulatory Care and Community Medicine, Lausanne, Switzerland."]}]}],"journalInfo":[{"volume":["11"],"journalIssueId":["2384654"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["New microbes and new infections"],"ISOAbbreviation":["New Microbes New Infect"],"medlineAbbreviation":["New Microbes New Infect"],"NLMid":["101624750"],"ESSN":["2052-2975"]}]}],"pubYear":["2016"],"pageInfo":["6-7"],"abstractText":["Zika virus is an emerging flavivirus that is following the path of dengue and chikungunya. The three Aedes-borne viruses cause simultaneous outbreaks with similar clinical manifestations which represents a diagnostic challenge in ill returning travellers. We report the first Zika virus infection case imported to Switzerland and present a diagnostic algorithm."],"affiliation":["Infectious Diseases Service, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["outbreak","Dengue","Chikungunya","Diagnostic Algorithm","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4786754?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4786754"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.nmni.2016.02.003"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-03-23"],"dateOfCreation":["2016-03-23"],"dateOfRevision":["2016-03-25"],"electronicPublicationDate":["2016-02-11"],"firstPublicationDate":["2016-02-11"],"cprojectID":"PMC4786754"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrIehOUS2orL1IPm","_score":1,"_source":{"id":["27081477"],"source":["MED"],"pmid":["27081477"],"pmcid":["PMC4813633"],"title":["Post-chikungunya chronic inflammatory rheumatism: results from a retrospective follow-up study of 283 adult and child cases in La Virginia, Risaralda, Colombia."],"authorString":["Rodriguez-Morales AJ, Gil-Restrepo AF, Ramírez-Jaramillo V, Montoya-Arias CP, Acevedo-Mendoza WF, Bedoya-Arias JE, Chica-Quintero LA, Murillo-García DR, García-Robledo JE, Castrillón-Spitia JD, Londoño JJ, Bedoya-Rendón HD, Cárdenas-Pérez Jde J, Cardona-Ospina JA, Lagos-Grisales GJ."],"authorList":[{"author":[{"fullName":["Rodriguez-Morales AJ"],"firstName":["Alfonso J"],"lastName":["Rodriguez-Morales"],"initials":["AJ"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia; Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Bucaramanga, Colombia; Committee on Zoonoses and Haemorrhagic Fevers, Asociación Colombiana de Infectología (ACIN), Bogota, Colombia; Working Group on Zoonoses, International Society for Chemotherapy, Aberdeen, UK."]},{"fullName":["Gil-Restrepo AF"],"firstName":["Andrés F"],"lastName":["Gil-Restrepo"],"initials":["AF"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Ramírez-Jaramillo V"],"firstName":["Valeria"],"lastName":["Ramírez-Jaramillo"],"initials":["V"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Montoya-Arias CP"],"firstName":["Cindy P"],"lastName":["Montoya-Arias"],"initials":["CP"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Acevedo-Mendoza WF"],"firstName":["Wilmer F"],"lastName":["Acevedo-Mendoza"],"initials":["WF"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Bedoya-Arias JE"],"firstName":["Juan E"],"lastName":["Bedoya-Arias"],"initials":["JE"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Chica-Quintero LA"],"firstName":["Laura A"],"lastName":["Chica-Quintero"],"initials":["LA"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Murillo-García DR"],"firstName":["David R"],"lastName":["Murillo-García"],"initials":["DR"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["García-Robledo JE"],"firstName":["Juan E"],"lastName":["García-Robledo"],"initials":["JE"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia; School of Medicine, Faculty of Health Sciences, Universidad ICESI, Cali, Colombia."]},{"fullName":["Castrillón-Spitia JD"],"firstName":["Juan D"],"lastName":["Castrillón-Spitia"],"initials":["JD"],"affiliation":["ESE Hospital San Pedro y San Pablo, La Virginia, Colombia."]},{"fullName":["Londoño JJ"],"firstName":["Jose J"],"lastName":["Londoño"],"initials":["JJ"],"affiliation":["School of Medicine, Faculty of Health Sciences, Universidad ICESI, Cali, Colombia."]},{"fullName":["Bedoya-Rendón HD"],"firstName":["Hector D"],"lastName":["Bedoya-Rendón"],"initials":["HD"],"affiliation":["ESE Hospital San Pedro y San Pablo, La Virginia, Colombia."]},{"fullName":["Cárdenas-Pérez Jde J"],"firstName":["Javier de Jesús"],"lastName":["Cárdenas-Pérez"],"initials":["Jde J"],"affiliation":["ESE Hospital San Pedro y San Pablo, La Virginia, Colombia."]},{"fullName":["Cardona-Ospina JA"],"firstName":["Jaime A"],"lastName":["Cardona-Ospina"],"initials":["JA"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]},{"fullName":["Lagos-Grisales GJ"],"firstName":["Guillermo J"],"lastName":["Lagos-Grisales"],"initials":["GJ"],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["360"],"abstractText":["There are limited studies in Latin America regarding the chronic consequences of the Chikungunya virus (CHIK), such as post-CHIK chronic inflammatory rheumatism (pCHIK-CIR). We assessed the largest cohort so far of pCHIK-CIR in Latin America, at the municipality of La Virginia, Risaralda, a new endemic area of CHIK in Colombia.We conducted a cohort retrospective study in Colombia of 283 patients diagnosed with CHIK that persisted with pCHIK-CIR after a minimum of 6 weeks and up to a maximum of 26.1 weeks. pCHIK cases were identified according to validated criteria via telephone.Of the total CHIK-infected subjects, 152 (53.7%) reported persistent rheumatological symptoms (pCHIK-CIR). All of these patients reported joint pains (chronic polyarthralgia, pCHIK-CPA), 49.5% morning stiffness, 40.6% joint edema, and 16.6% joint redness. Of all patients, 19.4% required and attended for care prior to the current study assessment (1.4% consulting rheumatologists). Significant differences in the frequency were observed according to age groups and gender. Patients aged >40 years old required more medical attention (39.5%) than those ≤40 years-old (12.1%) (RR=4.748, 95%CI 2.550-8.840).According to our results, at least half of the patients with CHIK developed chronic rheumatologic sequelae, and from those with pCHIK-CPA, nearly half presented clinical symptoms consistent with inflammatory forms of the disease. These results support previous estimates obtained from pooled data of studies in La Reunion (France) and India and are consistent with the results published previously from other Colombian cohorts in Venadillo (Tolima) and Since (Sucre)."],"affiliation":["Public Health and Infection Research Group, Faculty of Health Sciences,, Universidad Tecnológica de Pereira, Pereira, Colombia; Organización Latinoamericana para el Fomento de la Investigación en Salud (OLFIS), Bucaramanga, Colombia; Committee on Zoonoses and Haemorrhagic Fevers, Asociación Colombiana de Infectología (ACIN), Bogota, Colombia; Working Group on Zoonoses, International Society for Chemotherapy, Aberdeen, UK."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4813633?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4813633"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-04-15"],"dateOfCreation":["2016-04-15"],"dateOfRevision":["2016-04-17"],"electronicPublicationDate":["2016-03-16"],"firstPublicationDate":["2016-03-16"],"cprojectID":"PMC4813633"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrI6J9hGurOzZIZf","_score":1,"_source":{"id":["27158456"],"source":["MED"],"pmid":["27158456"],"pmcid":["PMC4837983"],"title":["The academic, economic and societal impacts of Open Access: an evidence-based review."],"authorString":["Tennant JP, Waldner F, Jacques DC, Masuzzo P, Collister LB, Hartgerink CH."],"authorList":[{"author":[{"fullName":["Tennant JP"],"firstName":["Jonathan P"],"lastName":["Tennant"],"initials":["JP"],"affiliation":["Department of Earth Science and Engineering, Imperial College London, London, UK."]},{"fullName":["Waldner F"],"firstName":["François"],"lastName":["Waldner"],"initials":["F"],"affiliation":["Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium."]},{"fullName":["Jacques DC"],"firstName":["Damien C"],"lastName":["Jacques"],"initials":["DC"],"affiliation":["Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgium."]},{"fullName":["Masuzzo P"],"firstName":["Paola"],"lastName":["Masuzzo"],"initials":["P"],"affiliation":["Medical Biotechnology Center, VIB, Ghent, Belgium; Department of Biochemistry, Ghent University, Ghent, Belgium."]},{"fullName":["Collister LB"],"firstName":["Lauren B"],"lastName":["Collister"],"initials":["LB"],"affiliation":["University Library System, University of Pittsburgh, Pittsburgh, PA, USA."]},{"fullName":["Hartgerink CH"],"firstName":["Chris H J"],"lastName":["Hartgerink"],"initials":["CH"],"affiliation":["Department of Methodology and Statistics, Tilburg University, Tilburg, Netherlands."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["632"],"abstractText":["Ongoing debates surrounding Open Access to the scholarly literature are multifaceted and complicated by disparate and often polarised viewpoints from engaged stakeholders. At the current stage, Open Access has become such a global issue that it is critical for all involved in scholarly publishing, including policymakers, publishers, research funders, governments, learned societies, librarians, and academic communities, to be well-informed on the history, benefits, and pitfalls of Open Access. In spite of this, there is a general lack of consensus regarding the advantages or disadvantages of Open Access at multiple levels. This review aims to to be a resource for current knowledge on the impacts of Open Access by synthesizing important research in three major areas of impact: academic, economic and societal. While there is clearly much scope for additional research, several key trends are identified, including a broad citation advantage for researchers who publish openly, as well as additional benefits to the non-academic dissemination of their work. The economic case for Open Access is less well-understood, although it is clear that access to the research literature is key for innovative enterprises, and a range of governmental and non-governmental services. Furthermore, Open Access has the potential to save publishers and research funders considerable amounts of financial resources. The social case for Open Access is strong, in particular for advancing citizen science initiatives, and leveling the playing field for researchers in developing countries. Open Access supersedes all potential alternative modes of access to the scholarly literature through enabling unrestricted re-use, and long-term stability independent of financial constraints of traditional publishers that impede knowledge sharing. Open Access remains only one of the multiple challenges that the scholarly publishing system is currently facing. Yet, it provides one foundation for increasing engagement with researchers regarding ethical standards of publishing. We recommend that Open Access supporters focus their efforts on working to establish viable new models and systems of scholarly communication, rather than trying to undermine the existing ones as part of the natural evolution of the scholarly ecosystem. Based on this, future research should investigate the wider impacts of an ecosystem-wide transformation to a system of Open Research."],"affiliation":["Department of Earth Science and Engineering, Imperial College London, London, UK."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837983?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837983"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-05-09"],"dateOfCreation":["2016-05-09"],"dateOfRevision":["2016-05-11"],"electronicPublicationDate":["2016-04-11"],"firstPublicationDate":["2016-04-11"],"cprojectID":"PMC4837983"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrMnhOUS2orL1IPw","_score":1,"_source":{"id":["27093158"],"source":["MED"],"pmid":["27093158"],"pmcid":["PMC4836712"],"doi":["10.1371/journal.pntd.0004682"],"title":["Characterization of Lethal Zika Virus Infection in AG129 Mice."],"authorString":["Aliota MT, Caine EA, Walker EC, Larkin KE, Camacho E, Osorio JE."],"authorList":[{"author":[{"fullName":["Aliota MT"],"firstName":["Matthew T"],"lastName":["Aliota"],"initials":["MT"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Caine EA"],"firstName":["Elizabeth A"],"lastName":["Caine"],"initials":["EA"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Walker EC"],"firstName":["Emma C"],"lastName":["Walker"],"initials":["EC"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Larkin KE"],"firstName":["Katrina E"],"lastName":["Larkin"],"initials":["KE"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Camacho E"],"firstName":["Erwin"],"lastName":["Camacho"],"initials":["E"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]},{"fullName":["Osorio JE"],"firstName":["Jorge E"],"lastName":["Osorio"],"initials":["JE"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."]}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004682"],"abstractText":["Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever, which often is accompanied by maculopapular rash, headache, and myalgia. During the current outbreak in South America, ZIKV infection during pregnancy has been hypothesized to cause microcephaly and other diseases. The detection of ZIKV in fetal brain tissue supports this hypothesis. Because human infections with ZIKV historically have remained sporadic and, until recently, have been limited to small-scale epidemics, neither the disease caused by ZIKV nor the molecular determinants of virulence and/or pathogenicity have been well characterized. Here, we describe a small animal model for wild-type ZIKV of the Asian lineage.Using mice deficient in interferon α/β and Ɣ receptors (AG129 mice), we report that these animals were highly susceptible to ZIKV infection and disease, succumbing within seven to eight days. Rapid viremic dissemination was observed in visceral organs and brain; but only was associated with severe pathologies in the brain and muscle. Finally, these results were consistent across challenge routes, age of mice, and inoculum doses. These data represent a mouse model for ZIKV that is not dependent on adapting ZIKV to intracerebral passage in mice.Foot pad injection of AG129 mice with ZIKV represents a biologically relevant model for studying ZIKV infection and disease development following wild-type virus inoculation without the requirement for adaptation of the virus or intracerebral delivery of the virus. This newly developed Zika disease model can be exploited to identify determinants of ZIKV virulence and reveal molecular mechanisms that control the virus-host interaction, providing a framework for rational design of acute phase therapeutics and for vaccine efficacy testing."],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["#AI117413-01"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["R21 AI117413"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["NIHT32AI055397"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Muscles"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Brain"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Mice, Knockout"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Viremia"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Models, Animal"]},{"majorTopic_YN":["N"],"descriptorName":["Death"]},{"majorTopic_YN":["N"],"descriptorName":["Receptors, Interferon"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DF"],"qualifierName":["deficiency"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Receptor, Interferon alpha-beta"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DF"],"qualifierName":["deficiency"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["interferon gamma receptor"],"registryNumber":["0"]},{"name":["Receptors, Interferon"],"registryNumber":["0"]},{"name":["Receptor, Interferon alpha-beta"],"registryNumber":["156986-95-7"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4836712?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4836712"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004682"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27214379"],"source":["MED"],"reference":["PLoS Negl Trop Dis. 2016 May;10(5):e0004750"],"type":["Erratum in"],"orderIn":["41"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-20"],"dateOfRevision":["2016-09-09"],"electronicPublicationDate":["2016-04-19"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4836712"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrMFhOUS2orL1IPu","_score":1,"_source":{"id":["27075009"],"source":["MED"],"pmid":["27075009"],"pmcid":["PMC4830901"],"doi":["10.1136/bmj.i1901"],"title":["Clinical features and neuroimaging (CT and MRI) findings in presumed Zika virus related congenital infection and microcephaly: retrospective case series study."],"authorString":["de Fatima Vasco Aragao M, van der Linden V, Brainer-Lima AM, Coeli RR, Rocha MA, Sobral da Silva P, Durce Costa Gomes de Carvalho M, van der Linden A, Cesario de Holanda A, Valenca MM."],"authorList":[{"author":[{"fullName":["de Fatima Vasco Aragao M"],"firstName":["Maria"],"lastName":["de Fatima Vasco Aragao"],"initials":["M"],"affiliation":["Centro Diagnostico Multimagem, Rua Frei Matias Tevis, 194, Ilha do Leite Recife Pernambuco 52010-450, Brazil; Medical School, Mauricio de Nassau University, Recife, Pernambuco, Brazil fatima.vascoaragao@gmail.com."]},{"fullName":["van der Linden V"],"firstName":["Vanessa"],"lastName":["van der Linden"],"initials":["V"],"affiliation":["Association for Assistance of Disabled Children, Recife, Brazil; Barão de Lucena Hospital, Recife, Brazil."]},{"fullName":["Brainer-Lima AM"],"firstName":["Alessandra Mertens"],"lastName":["Brainer-Lima"],"initials":["AM"],"affiliation":["PROCAPE, University of Pernambuco, Recife, Brazil."]},{"fullName":["Coeli RR"],"firstName":["Regina Ramos"],"lastName":["Coeli"],"initials":["RR"],"affiliation":["Oswaldo Cruz University Hospital, Recife, Brazil."]},{"fullName":["Rocha MA"],"firstName":["Maria Angela"],"lastName":["Rocha"],"initials":["MA"],"affiliation":["Oswaldo Cruz University Hospital, Recife, Brazil."]},{"fullName":["Sobral da Silva P"],"firstName":["Paula"],"lastName":["Sobral da Silva"],"initials":["P"],"affiliation":["Oswaldo Cruz University Hospital, Recife, Brazil."]},{"fullName":["Durce Costa Gomes de Carvalho M"],"firstName":["Maria"],"lastName":["Durce Costa Gomes de Carvalho"],"initials":["M"],"affiliation":["Oswaldo Cruz University Hospital, Recife, Brazil."]},{"fullName":["van der Linden A"],"firstName":["Ana"],"lastName":["van der Linden"],"initials":["A"],"affiliation":["Prof Fernando Figueira Integral Medicine Institute, Recife, Brazil."]},{"fullName":["Cesario de Holanda A"],"firstName":["Arthur"],"lastName":["Cesario de Holanda"],"initials":["A"],"affiliation":["Medical School, Federal University of Pernambuco, Recife, Brazil."]},{"fullName":["Valenca MM"],"firstName":["Marcelo Moraes"],"lastName":["Valenca"],"initials":["MM"],"affiliation":["Neurology and Neurosurgery, Federal University of Pernambuco, Recife, Brazil."]}]}],"journalInfo":[{"volume":["353"],"journalIssueId":["2394075"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMJ (Clinical research ed.)"],"ISOAbbreviation":["BMJ"],"medlineAbbreviation":["BMJ"],"NLMid":["8900488"],"ISSN":["0959-8138"],"ESSN":["1756-1833"]}]}],"pubYear":["2016"],"pageInfo":["i1901"],"abstractText":["To report radiological findings observed in computed tomography (CT) and magnetic resonance imaging (MRI) scans of the first cases of congenital infection and microcephaly presumably associated with the Zika virus in the current Brazilian epidemic.Retrospective study with a case series.Association for Assistance of Disabled Children (AACD), Pernambuco state, Brazil.23 children with a diagnosis of congenital infection presumably associated with the Zika virus during the Brazilian microcephaly epidemic.Types of abnormalities and the radiological pattern of lesions identified on CT and MRI brain scans.Six of the 23 children tested positive for IgM antibodies to Zika virus in cerebrospinal fluid. The other 17 children met the protocol criteria for congenital infection presumably associated with the Zika virus, even without being tested for IgM antibodies to the virus--the test was not yet available on a routine basis. Of the 23 children, 15 underwent CT, seven underwent both CT and MRI, and one underwent MRI. Of the 22 children who underwent CT, all had calcifications in the junction between cortical and subcortical white matter, 21 (95%) had malformations of cortical development, 20 (91%) had a decreased brain volume, 19 (86%) had ventriculomegaly, and 11 (50%) had hypoplasia of the cerebellum or brainstem. Of the eight children who underwent MRI, all had calcifications in the junction between cortical and subcortical white matter, malformations of cortical development occurring predominantly in the frontal lobes, and ventriculomegaly. Seven of the eight (88%) children had enlarged cisterna magna, seven (88%) delayed myelination, and six each (75%) a moderate to severe decrease in brain volume, simplified gyral pattern, and abnormalities of the corpus callosum (38% hypogenesis and 38% hypoplasia). Malformations were symmetrical in 75% of the cases.Severe cerebral damage was found on imaging in most of the children in this case series with congenital infection presumably associated with the Zika virus. The features most commonly found were brain calcifications in the junction between cortical and subcortical white matter associated with malformations of cortical development, often with a simplified gyral pattern and predominance of pachygyria or polymicrogyria in the frontal lobes. Additional findings were enlarged cisterna magna, abnormalities of corpus callosum (hypoplasia or hypogenesis), ventriculomegaly, delayed myelination, and hypoplasia of the cerebellum and the brainstem."],"affiliation":["Centro Diagnostico Multimagem, Rua Frei Matias Tevis, 194, Ilha do Leite Recife Pernambuco 52010-450, Brazil; Medical School, Mauricio de Nassau University, Recife, Pernambuco, Brazil fatima.vascoaragao@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Video-Audio Media"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Brain"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]},{"abbreviation":["RA"],"qualifierName":["radiography"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Corpus Callosum"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["RA"],"qualifierName":["radiography"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cisterna Magna"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["RA"],"qualifierName":["radiography"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Nerve Fibers, Myelinated"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["RA"],"qualifierName":["radiography"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Pregnancy Complications, Infectious"]},{"majorTopic_YN":["N"],"descriptorName":["Microcephaly"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Hydrocephalus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Calcinosis"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin M"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CF"],"qualifierName":["cerebrospinal fluid"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Tomography, X-Ray Computed"]},{"majorTopic_YN":["N"],"descriptorName":["Magnetic Resonance Imaging"]},{"majorTopic_YN":["N"],"descriptorName":["Organ Size"]},{"majorTopic_YN":["N"],"descriptorName":["Retrospective Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Pregnancy"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Infant"]},{"majorTopic_YN":["N"],"descriptorName":["Infant, Newborn"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Malformations of Cortical Development"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Agenesis of Corpus Callosum"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["White Matter"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["RA"],"qualifierName":["radiography"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrOMJ9hGurOzZIZq","_score":1,"_source":{"id":["27115737"],"source":["MED"],"pmid":["27115737"],"pmcid":["PMC4846050"],"doi":["10.1371/journal.pntd.0004664"],"title":["Spread of the Invasive Mosquitoes Aedes aegypti and Aedes albopictus in the Black Sea Region Increases Risk of Chikungunya, Dengue, and Zika Outbreaks in Europe."],"authorString":["Akiner MM, Demirci B, Babuadze G, Robert V, Schaffner F."],"authorList":[{"author":[{"fullName":["Akiner MM"],"firstName":["Muhammet M"],"lastName":["Akiner"],"initials":["MM"],"affiliation":["Recep Tayyip Erdogan University, Faculty of Arts and Sciences, Department of Biology, Fener, Rize, Turkey."]},{"fullName":["Demirci B"],"firstName":["Berna"],"lastName":["Demirci"],"initials":["B"],"affiliation":["Kafkas University, Molecular Biology and Genetics Department, Kars, Turkey."]},{"fullName":["Babuadze G"],"firstName":["Giorgi"],"lastName":["Babuadze"],"initials":["G"],"affiliation":["Division of 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Recently, the tetravalent chimeric live attenuated Dengue vaccine Dengvaxia® was approved for use in several dengue endemic countries. In general, live attenuated vaccines (LAV) are very efficacious and offer long-lasting immunity against virus-induced disease. Rationally designed LAVs can be generated through reverse genetics technology, a method of generating infectious recombinant viruses from full length cDNA contained in bacterial plasmids. In vitro transcribed (IVT) viral RNA from these infectious clones is transfected into susceptible cells to generate recombinant virus. However, the generation of full-length dengue virus cDNA clones can be difficult due to the genetic instability of viral sequences in bacterial plasmids. To circumvent the need for a single plasmid containing a full length cDNA, in vitro ligation of two or three cDNA fragments contained in separate plasmids can be used to generate a full-length dengue viral cDNA template. However, in vitro ligation of multiple fragments often yields low quality template for IVT reactions, resulting in inconsistent low yield RNA. These technical difficulties make recombinant virus recovery less efficient. In this study, we describe a simple, rapid and efficient method of using LONG-PCR to recover recombinant chimeric Yellow fever dengue (CYD) viruses as potential dengue vaccine candidates. Using this method, we were able to efficiently generate several viable recombinant viruses without introducing any artificial mutations into the viral genomes. 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrPq_8VqgcuJwvhu","_score":1,"_source":{"id":["27114968"],"source":["MED"],"pmid":["27114968"],"pmcid":["PMC4821090"],"doi":["10.4103/1947-2714.179112"],"title":["Zika Virus: A Global Threat to Humanity: A Comprehensive Review and Current Developments."],"authorString":["Hajra A, Bandyopadhyay D, Hajra SK."],"authorList":[{"author":[{"fullName":["Hajra A"],"firstName":["Adrija"],"lastName":["Hajra"],"initials":["A"],"affiliation":["Department of Internal Medicine, Institute of Post Graduate Medical Education and Research (IPGMER), Kolkata, West Bengal, India."]},{"fullName":["Bandyopadhyay D"],"firstName":["Dhrubajyoti"],"lastName":["Bandyopadhyay"],"initials":["D"],"affiliation":["Department of Accident and Emergency, Lady Hardinge Medical College, New Delhi, India."]},{"fullName":["Hajra SK"],"firstName":["Shyamal Kumar"],"lastName":["Hajra"],"initials":["SK"],"affiliation":["Consultant Physician, NMB Diagnostics, Serampore, Hooghly, West Bengal, India."]}]}],"journalInfo":[{"issue":["3"],"volume":["8"],"journalIssueId":["2401512"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["North American journal of medical sciences"],"ISOAbbreviation":["N Am J Med Sci"],"medlineAbbreviation":["N Am J Med Sci"],"NLMid":["101521411"],"ISSN":["2250-1541"],"ESSN":["1947-2714"]}]}],"pubYear":["2016"],"pageInfo":["123-128"],"abstractText":["At present, one of greatest concerns of medical personnel is Zika virus (ZIKV). Though it has been reported for quite a long time, its rapid emergence, new modes of transmission, and more importantly, the congenital anomalies associated with it have made the situation worse. It was first detected in 1947. After that, this infection was found in the countries of Africa as well as Asia. At present, interestingly it has been reported from Brazil. Microcephaly and intracranial calcification have been postulated to be related to maternal infection with this virus. Though it is asymptomatic in maximum number of cases, the serious complications of the infection should be prevented at the earliest. No specific treatment and vaccine are available till now. But research continues and hopefully, success is not far off. The right information about this infection should reach patients as well as physicians. It will prevent unnecessary panic. In August, Brazil is going to organize the Olympic and Paralympic Games and all eyes are now focused on this. In this review article, the authors have tried to focus on the important points about this infection. The data were gathered after searching for relevant articles published in PubMed, the World Health Organization's (WHO) website, Centers for Disease Control and Prevention's (CDC) website, and some other related websites on the Internet."],"affiliation":["Department of Internal Medicine, Institute of Post Graduate Medical Education and Research (IPGMER), Kolkata, West Bengal, India."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Pregnancy","Microcephaly","Aedes Mosquitoes","Zika Virus (Zikv)"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4821090"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/1947-2714.179112"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-26"],"dateOfCreation":["2016-04-26"],"dateOfRevision":["2016-04-28"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4821090"}}
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Lourenço Center for the Study of Emerging and Re-emerging Pathogens, Rutgers University-New Jersey Medical School, Newark, NJ, USA."]},{"fullName":["Freitas-Junior L"],"firstName":["Lucio"],"lastName":["Freitas-Junior"],"initials":["L"],"affiliation":["Chemical Biology and Screening Platform, Brazilian Laboratory of Biosciences (LNBio), CNPEM, Campinas, Brazil."]},{"fullName":["Muratov E"],"firstName":["Eugene"],"lastName":["Muratov"],"initials":["E"],"affiliation":["Laboratory for Molecular Modeling, Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, USA."]},{"fullName":["Siqueira-Neto J"],"firstName":["Jair"],"lastName":["Siqueira-Neto"],"initials":["J"],"affiliation":["Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, San Diego, CA, USA."]},{"fullName":["Williams AJ"],"firstName":["Antony J"],"lastName":["Williams"],"initials":["AJ"],"authorId":[{"_":"0000-0002-2668-4821","$":{"type":"ORCID"}}],"affiliation":["ChemConnector, Wake Forest, NC, USA."]},{"fullName":["Andrade C"],"firstName":["Carolina"],"lastName":["Andrade"],"initials":["C"],"authorId":[{"_":"0000-0003-0101-1492","$":{"type":"ORCID"}}],"affiliation":["LabMol - Laboratory for Molecular Modeling and Drug Design, Faculty of Pharmacy, Federal University of Goias, Goiânia, Brazil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9488-1870","$":{"type":"ORCID"}},{"_":"0000-0002-2668-4821","$":{"type":"ORCID"}},{"_":"0000-0003-0101-1492","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["150"],"abstractText":["The Zika virus (ZIKV) outbreak in the Americas has caused global concern that we may be on the brink of a healthcare crisis. The lack of research on ZIKV in the over 60 years that we have known about it has left us with little in the way of starting points for drug discovery. Our response can build on previous efforts with virus outbreaks and lean heavily on work done on other flaviviruses such as dengue virus. We provide some suggestions of what might be possible and propose an open drug discovery effort that mobilizes global science efforts and provides leadership, which thus far has been lacking. We also provide a listing of potential resources and molecules that could be prioritized for testing as in vitro assays for ZIKV are developed. We propose also that in order to incentivize drug discovery, a neglected disease priority review voucher should be available to those who successfully develop an FDA approved treatment. Learning from the response to the ZIKV, the approaches to drug discovery used and the success and failures will be critical for future infectious disease outbreaks."],"affiliation":["Collaborations in Chemistry Inc, Fuquay-Varina, NC, USA; Collaborations Pharmaceuticals Inc., Fuquay-Varina, NC, USA; Collaborative Drug Discovery Inc., Burlingame, CA, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","Dengue","Yellow Fever","drug discovery","Microcephaly","Flavivirus","Ebola","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841202?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841202"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8013.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-02"],"dateOfCreation":["2016-05-02"],"dateOfRevision":["2016-05-04"],"electronicPublicationDate":["2016-02-09"],"firstPublicationDate":["2016-02-09"],"cprojectID":"PMC4841202"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrPfJ9hGurOzZIZw","_score":1,"_source":{"id":["27143174"],"source":["MED"],"pmid":["27143174"],"pmcid":["PMC4855997"],"doi":["10.1590/1414-431x20165420"],"title":["Overview of Zika virus (ZIKV) infection in regards to the Brazilian epidemic."],"authorString":["Slavov SN, Otaguiri KK, Kashima S, Covas DT."],"authorList":[{"author":[{"fullName":["Slavov SN"],"firstName":["S N"],"lastName":["Slavov"],"initials":["SN"],"affiliation":["Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil."]},{"fullName":["Otaguiri KK"],"firstName":["K K"],"lastName":["Otaguiri"],"initials":["KK"],"affiliation":["Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil."]},{"fullName":["Kashima S"],"firstName":["S"],"lastName":["Kashima"],"initials":["S"],"affiliation":["Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil."]},{"fullName":["Covas DT"],"firstName":["D T"],"lastName":["Covas"],"initials":["DT"],"affiliation":["Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil."]}]}],"journalInfo":[{"issue":["5"],"volume":["49"],"journalIssueId":["2400032"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas / Sociedade Brasileira de Biofisica ... [et al.]"],"ISOAbbreviation":["Braz. J. Med. Biol. Res."],"medlineAbbreviation":["Braz J Med Biol Res"],"NLMid":["8112917"],"ISSN":["0100-879X"],"ESSN":["1414-431X"]}]}],"pubYear":["2016"],"pageInfo":["e5420"],"abstractText":["Zika virus (ZIKV), a mosquito-borne flavivirus, belongs to the Flaviviridae family, genus Flavivirus. ZIKV was initially isolated in 1947 from a sentinel monkey in the Zika forest, Uganda. Little clinical importance was attributed to ZIKV, once only few symptomatic cases were reported in some African and Southeast Asiatic countries. This situation changed in 2007, when a large outbreak was registered on the Yap Island, Micronesia, caused by the Asian ZIKV lineage. Between 2013 and 2014, ZIKV spread explosively and caused many outbreaks in different islands of the Southern Pacific Ocean and in 2015 autochthonous transmission was reported in Brazil. Currently, Brazil is the country with the highest number of ZIKV-positive cases in Latin America. Moreover, for the first time after the discovery of ZIKV, the Brazilian scientists are studying the possibility for the virus to cause severe congenital infection related to microcephaly and serious birth defects due to the time-spatial coincidence of the alarming increase of newborns with microcephaly and the Brazilian ZIKV epidemic. The present review summarizes recent information for ZIKV epidemiology, clinical picture, transmission, diagnosis and the consequences of this emerging virus in Brazil."],"affiliation":["Hemocentro de Ribeirão Preto, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4855997?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4855997"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/1414-431X20165420"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-04"],"dateOfRevision":["2016-05-10"],"electronicPublicationDate":["2016-04-29"],"firstPublicationDate":["2016-04-29"],"cprojectID":"PMC4855997"}}
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It is also evident that many of the nationally based governance bodies in this field do not have the legal powers or expertise to adjudicate on the complex issues, such as privacy and disclosure risks that are raised by cross-border data sharing. In addition, the conceptual underpinning of this research governance structure is based on the \"one researcher, one project, one jurisdiction\" model. In the conclusion of this paper, I lay out some preliminary ideas as to how this system has to change to accommodate research that is based on networks. 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqnehOUS2orL1IOY","_score":1,"_source":{"id":["25299181"],"source":["MED"],"pmid":["25299181"],"pmcid":["PMC4191940"],"doi":["10.1371/journal.pntd.0003188"],"title":["Aedes hensilli as a potential vector of Chikungunya and Zika viruses."],"authorString":["Ledermann JP, Guillaumot L, Yug L, Saweyog SC, Tided M, Machieng P, Pretrick M, Marfel M, Griggs A, Bel M, Duffy MR, Hancock WT, Ho-Chen T, Powers AM."],"authorList":[{"author":[{"fullName":["Ledermann JP"],"firstName":["Jeremy P"],"lastName":["Ledermann"],"initials":["JP"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America."]},{"fullName":["Guillaumot L"],"firstName":["Laurent"],"lastName":["Guillaumot"],"initials":["L"],"affiliation":["URE-Entomologie Medicale, Institut Pasteur de Nouvelle-Caledonie, Noumea, New Caledonia."]},{"fullName":["Yug L"],"firstName":["Lawrence"],"lastName":["Yug"],"initials":["L"],"affiliation":["Environmental Health Services, Division of Public Health, Department of Health Services, Pohnpei, Federated States of Micronesia."]},{"fullName":["Saweyog SC"],"firstName":["Steven C"],"lastName":["Saweyog"],"initials":["SC"],"affiliation":["National Food Safety Program, Department of Health and Social Affairs, Pohnpei, Federated States of Micronesia."]},{"fullName":["Tided M"],"firstName":["Mary"],"lastName":["Tided"],"initials":["M"],"affiliation":["Environmental Health Services, Division of Public Health, Department of Health Services, Pohnpei, Federated States of Micronesia."]},{"fullName":["Machieng P"],"firstName":["Paul"],"lastName":["Machieng"],"initials":["P"],"affiliation":["National Food Safety Program, Department of Health and Social Affairs, Pohnpei, Federated States of Micronesia."]},{"fullName":["Pretrick M"],"firstName":["Moses"],"lastName":["Pretrick"],"initials":["M"],"affiliation":["Department of Health, Education and Social Affairs, Pohnpei, Federated States of Micronesia."]},{"fullName":["Marfel M"],"firstName":["Maria"],"lastName":["Marfel"],"initials":["M"],"affiliation":["Wa'ab Community Health Center, Yap, Federated States of Micronesia."]},{"fullName":["Griggs A"],"firstName":["Anne"],"lastName":["Griggs"],"initials":["A"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America."]},{"fullName":["Bel M"],"firstName":["Martin"],"lastName":["Bel"],"initials":["M"],"affiliation":["Wa'ab Community Health Center, Yap, Federated States of Micronesia."]},{"fullName":["Duffy MR"],"firstName":["Mark R"],"lastName":["Duffy"],"initials":["MR"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America."]},{"fullName":["Hancock WT"],"firstName":["W Thane"],"lastName":["Hancock"],"initials":["WT"],"affiliation":["Wa'ab Community Health Center, Yap, Federated States of Micronesia."]},{"fullName":["Ho-Chen T"],"firstName":["Tai"],"lastName":["Ho-Chen"],"initials":["T"],"affiliation":["Epidemic Intelligence Service Field Assignments Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America."]},{"fullName":["Powers AM"],"firstName":["Ann M"],"lastName":["Powers"],"initials":["AM"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America."]}]}],"journalInfo":[{"issue":["10"],"volume":["8"],"journalIssueId":["2207097"],"dateOfPublication":["2014 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2014"],"printPublicationDate":["2014-10-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2014"],"pageInfo":["e3188"],"abstractText":["An epidemic of Zika virus (ZIKV) illness that occurred in July 2007 on Yap Island in the Federated States of Micronesia prompted entomological studies to identify both the primary vector(s) involved in transmission and the ecological parameters contributing to the outbreak. Larval and pupal surveys were performed to identify the major containers serving as oviposition habitat for the likely vector(s). Adult mosquitoes were also collected by backpack aspiration, light trap, and gravid traps at select sites around the capital city. The predominant species found on the island was Aedes (Stegomyia) hensilli. No virus isolates were obtained from the adult field material collected, nor did any of the immature mosquitoes that were allowed to emerge to adulthood contain viable virus or nucleic acid. Therefore, laboratory studies of the probable vector, Ae. hensilli, were undertaken to determine the likelihood of this species serving as a vector for Zika virus and other arboviruses. Infection rates of up to 86%, 62%, and 20% and dissemination rates of 23%, 80%, and 17% for Zika, chikungunya, and dengue-2 viruses respectively, were found supporting the possibility that this species served as a vector during the Zika outbreak and that it could play a role in transmitting other medically important arboviruses."],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, U.S. Gov't, P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Species Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Micronesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4191940?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4191940"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0003188"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["9"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-02-08"],"dateOfCreation":["2014-10-10"],"electronicPublicationDate":["2014-10-09"],"firstPublicationDate":["2014-10-09"],"cprojectID":"PMC4191940"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqodJ9hGurOzZIYT","_score":1,"_source":{"id":["26122427"],"source":["MED"],"pmid":["26122427"],"pmcid":["PMC4491199"],"doi":["10.1186/s13071-015-0936-9"],"title":["Current status of invasive mosquito surveillance in the UK."],"authorString":["Vaux AG, Medlock JM."],"authorList":[{"author":[{"fullName":["Vaux AG"],"firstName":["Alexander G C"],"lastName":["Vaux"],"initials":["AG"],"affiliation":["Medical Entomology & Zoonoses Ecology group, Emergency Response Department, Public Health England, Porton Down, Salisbury, SP4 0JG, United Kingdom. Alex.Vaux@phe.gov.uk."]},{"fullName":["Medlock JM"],"firstName":["Jolyon M"],"lastName":["Medlock"],"initials":["JM"],"affiliation":["Medical Entomology & Zoonoses Ecology group, Emergency Response Department, Public Health England, Porton Down, Salisbury, SP4 0JG, United Kingdom. Jolyon.Medlock@phe.gov.uk."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2261389"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2015"],"pageInfo":["351"],"abstractText":["Non-native invasive mosquitoes have for many years made incursions into Europe, and are now established in many European countries. The continued European importation of potential vectors and their expansion within Europe increases their potential for importation and establishment in the UK. Coupled with increasing numbers of returning dengue and chikungunya infected travellers, the potential exists for transmission of vector borne disease in new regions.To ensure a cost-effective risk assessment and preparedness strategy the UK employs a multi-faceted approach to surveillance for non-native Aedes mosquitoes, including passive and active surveillance strategies at a local, regional, and national level. Passive surveillance, including a national mosquito recording scheme and local authority nuisance biting reporting, are combined with targeted active surveillance at seaports, airports, used tyre importers, and motorway service stations.There is no evidence to date that any invasive Aedes species (e.g., Aedes albopictus, Aedes japonicus, Aedes aegypti) occur in the UK despite sharing many of the same routes that have been found to have facilitated their entry into other countries.This paper sets in context the UK approaches with other European countries and those recommended by the ECDC. It also highlights future UK strategies to enhance surveillance for non-native mosquitoes to help ensure that incursions can be managed, and these mosquitoes do not establish and public health is protected. Focus will be given to increasing the number of submissions of mosquitoes to passive surveillance schemes and maintaining active surveillance efforts at key routes of potential importation."],"affiliation":["Medical Entomology & Zoonoses Ecology group, Emergency Response Department, Public Health England, Porton Down, Salisbury, SP4 0JG, United Kingdom. Alex.Vaux@phe.gov.uk."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Data Collection"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Mosquito Control"]},{"majorTopic_YN":["N"],"descriptorName":["Great Britain"]},{"majorTopic_YN":["N"],"descriptorName":["Epidemiological Monitoring"]},{"majorTopic_YN":["N"],"descriptorName":["Animal Distribution"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4491199?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4491199"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-015-0936-9"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-22"],"dateOfCreation":["2015-07-04"],"dateOfRevision":["2015-07-08"],"electronicPublicationDate":["2015-06-30"],"firstPublicationDate":["2015-06-30"],"cprojectID":"PMC4491199"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqn8hOUS2orL1IOb","_score":1,"_source":{"id":["25522325"],"source":["MED"],"pmid":["25522325"],"pmcid":["PMC4270494"],"doi":["10.1371/journal.pntd.0003416"],"title":["Universal single-probe RT-PCR assay for diagnosis of dengue virus infections."],"authorString":["Alm E, Lesko B, Lindegren G, Ahlm C, Söderholm S, Falk KI, Lagerqvist N."],"authorList":[{"author":[{"fullName":["Alm E"],"firstName":["Erik"],"lastName":["Alm"],"initials":["E"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden."]},{"fullName":["Lesko B"],"firstName":["Birgitta"],"lastName":["Lesko"],"initials":["B"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden; Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden."]},{"fullName":["Lindegren G"],"firstName":["Gunnel"],"lastName":["Lindegren"],"initials":["G"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden."]},{"fullName":["Ahlm C"],"firstName":["Clas"],"lastName":["Ahlm"],"initials":["C"],"affiliation":["Department of Clinical Microbiology, Umeå University, Umeå, Sweden."]},{"fullName":["Söderholm S"],"firstName":["Sandra"],"lastName":["Söderholm"],"initials":["S"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden."]},{"fullName":["Falk KI"],"firstName":["Kerstin I"],"lastName":["Falk"],"initials":["KI"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden."]},{"fullName":["Lagerqvist N"],"firstName":["Nina"],"lastName":["Lagerqvist"],"initials":["N"],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden; Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Stockholm, Sweden."]}]}],"journalInfo":[{"issue":["12"],"volume":["8"],"journalIssueId":["2224174"],"dateOfPublication":["2014 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2014"],"printPublicationDate":["2014-12-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2014"],"pageInfo":["e3416"],"abstractText":["Dengue is a mosquito-borne viral disease that has become more prevalent in the last few decades. Most patients are viremic when they present with symptoms, and early diagnosis of dengue is important in preventing severe clinical complications associated with this disease and also represents a key factor in differential diagnosis. Here, we designed and validated a hydrolysis-probe-based one-step real-time RT-PCR assay that targets the genomes of dengue virus serotypes 1-4.The primers and probe used in our RT-PCR assay were designed to target the 3' untranslated region of all complete genome sequences of dengue virus available in GenBank (n = 3,305). Performance of the assay was evaluated using in vitro transcribed RNA, laboratory-adapted virus strains, external control panels, and clinical specimens. The linear dynamic range was found to be 104-1011 GCE/mL, and the detection limit was between 6.0×102 and 1.1×103 GCE/mL depending on target sequence. The assay did not cross-react with human RNA, nor did it produce false-positive results for other human pathogenic flaviviruses or clinically important etiological agents of febrile illnesses. We used clinical serum samples obtained from returning travelers with dengue-compatible symptomatology (n = 163) to evaluate the diagnostic relevance of our assay, and laboratory diagnosis performed by the RT-PCR assay had 100% positive agreement with diagnosis performed by NS1 antigen detection. In a retrospective evaluation including 60 archived serum samples collected from confirmed dengue cases 1-9 days after disease onset, the RT-PCR assay detected viral RNA up to 9 days after appearance of symptoms.The validation of the RT-PCR assay presented here indicates that this technique can be a reliable diagnostic tool, and hence we suggest that it be introduced as the method of choice during the first 5 days of dengue symptoms."],"affiliation":["Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Retrospective Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4270494?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4270494"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0003416"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-12-21"],"dateOfCreation":["2014-12-19"],"dateOfRevision":["2014-12-27"],"electronicPublicationDate":["2014-12-18"],"firstPublicationDate":["2014-12-18"],"cprojectID":"PMC4270494"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoohOUS2orL1IOj","_score":1,"_source":{"id":["25937916"],"source":["MED"],"pmid":["25937916"],"pmcid":["PMC4409421"],"doi":["10.1002/ece3.1477"],"title":["Temporal patterns in Saturnidae (silk moth) and Sphingidae (hawk moth) assemblages in protected forests of central Uganda."],"authorString":["Akite P, Telford RJ, Waring P, Akol AM, Vandvik V."],"authorList":[{"author":[{"fullName":["Akite P"],"firstName":["Perpetra"],"lastName":["Akite"],"initials":["P"],"authorId":[{"_":"0000-0002-0302-1822","$":{"type":"ORCID"}}],"affiliation":["Department of Biological Sciences, Makerere University Kampala, Uganda ; Department of Biology, University of Bergen Bergen, Norway."]},{"fullName":["Telford RJ"],"firstName":["Richard J"],"lastName":["Telford"],"initials":["RJ"],"affiliation":["Department of Biology, University of Bergen Bergen, Norway."]},{"fullName":["Waring P"],"firstName":["Paul"],"lastName":["Waring"],"initials":["P"],"affiliation":["Windall View Werrington, Peterborough, UK."]},{"fullName":["Akol AM"],"firstName":["Anne M"],"lastName":["Akol"],"initials":["AM"],"affiliation":["Department of Biological Sciences, Makerere University Kampala, Uganda."]},{"fullName":["Vandvik V"],"firstName":["Vigdis"],"lastName":["Vandvik"],"initials":["V"],"affiliation":["Department of Biology, University of Bergen Bergen, Norway."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0302-1822","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["5"],"journalIssueId":["2279902"],"dateOfPublication":["2015 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2015"],"printPublicationDate":["2015-04-01"],"journal":[{"title":["Ecology and evolution"],"ISOAbbreviation":["Ecol Evol"],"medlineAbbreviation":["Ecol Evol"],"NLMid":["101566408"],"ESSN":["2045-7758"]}]}],"pubYear":["2015"],"pageInfo":["1746-1757"],"abstractText":["Forest-dependent biodiversity is threatened throughout the tropics by habitat loss and land-use intensification of the matrix habitats. We resampled historic data on two moth families, known to play central roles in many ecosystem processes, to evaluate temporal changes in species richness and community structure in three protected forests in central Uganda in a rapidly changing matrix. Our results show some significant declines in the moth species richness and the relative abundance and richness of forest-dependent species over the last 20-40 years. The observed changes in species richness and composition among different forests, ecological types, and moth groups highlight the need to repeatedly monitor biodiversity even within protected and relatively intact forests."],"affiliation":["Department of Biological Sciences, Makerere University Kampala, Uganda ; Department of Biology, University of Bergen Bergen, Norway."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Lepidoptera","Resampling","Forest Degradation","Extinction Debt","Compositional Change","Matrix Intensification","Species Decline"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4409421?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4409421"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1002/ece3.1477"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-05-04"],"dateOfCreation":["2015-05-04"],"dateOfRevision":["2015-05-07"],"electronicPublicationDate":["2015-03-25"],"firstPublicationDate":["2015-03-25"],"cprojectID":"PMC4409421"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSSJ9hGurOzZIXk","_score":1,"_source":{"id":["13713265"],"source":["MED"],"pmid":["13713265"],"pmcid":["PMC2604178"],"title":["Applications of primary cell cultures in the study of animal viruses. II. Variations in host responses to infection by certain arthropod-borne viruses."],"authorString":["HENDERSON JR."],"authorList":[{"author":[{"fullName":["HENDERSON JR"],"lastName":["HENDERSON"],"initials":["JR"]}]}],"journalInfo":[{"volume":["33"],"journalIssueId":["266516"],"dateOfPublication":["1961 Apr"],"monthOfPublication":["4"],"yearOfPublication":["1961"],"printPublicationDate":["1961-04-01"],"journal":[{"title":["The Yale journal of biology and medicine"],"ISOAbbreviation":["Yale J Biol Med"],"medlineAbbreviation":["Yale J Biol Med"],"NLMid":["0417414"],"ISSN":["0044-0086"],"ESSN":["1551-4056"]}]}],"pubYear":["1961"],"pageInfo":["350-358"],"abstractText":[" "],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Viruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EH"],"qualifierName":["ethnology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Tissue Culture Techniques"]}]}],"keywordList":[{"keyword":["Tissue culture","Viruses/culture"]}],"subsetList":[{"subset":[{"code":["OM"],"name":["pre-1966 citations"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2604178?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2604178"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=13713265&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=13713265"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["5"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["1998-11-01"],"dateOfCreation":["1961-12-01"],"dateOfRevision":["2012-04-16"],"firstPublicationDate":["1961-04-01"],"cprojectID":"PMC2604178"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSehOUS2orL1IN6","_score":1,"_source":{"id":["23967358"],"source":["MED"],"pmid":["23967358"],"pmcid":["PMC3744434"],"doi":["10.1371/journal.pntd.0002349"],"title":["Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray."],"authorString":["Grubaugh ND, McMenamy SS, Turell MJ, Lee JS."],"authorList":[{"author":[{"fullName":["Grubaugh ND"],"firstName":["Nathan D"],"lastName":["Grubaugh"],"initials":["ND"],"affiliation":["Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, United States of America. nathan.grubaugh@colostate.edu"]},{"fullName":["McMenamy SS"],"firstName":["Scott S"],"lastName":["McMenamy"],"initials":["SS"]},{"fullName":["Turell MJ"],"firstName":["Michael J"],"lastName":["Turell"],"initials":["MJ"]},{"fullName":["Lee JS"],"firstName":["John S"],"lastName":["Lee"],"initials":["JS"],"authorId":[{"_":"0000-0001-7605-1814","$":{"type":"ORCID"}}]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7605-1814","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["7"],"journalIssueId":["2070282"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2013"],"pageInfo":["e2349"],"abstractText":["BACKGROUND: Arthropod-borne viruses are important emerging pathogens world-wide. Viruses transmitted by mosquitoes, such as dengue, yellow fever, and Japanese encephalitis viruses, infect hundreds of millions of people and animals each year. Global surveillance of these viruses in mosquito vectors using molecular based assays is critical for prevention and control of the associated diseases. Here, we report an oligonucleotide DNA microarray design, termed ArboChip5.1, for multi-gene detection and identification of mosquito-borne RNA viruses from the genera Flavivirus (family Flaviviridae), Alphavirus (Togaviridae), Orthobunyavirus (Bunyaviridae), and Phlebovirus (Bunyaviridae). METHODOLOGY/PRINCIPAL FINDINGS: The assay utilizes targeted PCR amplification of three genes from each virus genus for electrochemical detection on a portable, field-tested microarray platform. Fifty-two viruses propagated in cell-culture were used to evaluate the specificity of the PCR primer sets and the ArboChip5.1 microarray capture probes. The microarray detected all of the tested viruses and differentiated between many closely related viruses such as members of the dengue, Japanese encephalitis, and Semliki Forest virus clades. Laboratory infected mosquitoes were used to simulate field samples and to determine the limits of detection. Additionally, we identified dengue virus type 3, Japanese encephalitis virus, Tembusu virus, Culex flavivirus, and a Quang Binh-like virus from mosquitoes collected in Thailand in 2011 and 2012. CONCLUSIONS/SIGNIFICANCE: We demonstrated that the described assay can be utilized in a comprehensive field surveillance program by the broad-range amplification and specific identification of arboviruses from infected mosquitoes. Furthermore, the microarray platform can be deployed in the field and viral RNA extraction to data analysis can occur in as little as 12 h. The information derived from the ArboChip5.1 microarray can help to establish public health priorities, detect disease outbreaks, and evaluate control programs."],"affiliation":["Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, United States of America. nathan.grubaugh@colostate.edu"],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA Viruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Oligonucleotide Array Sequence Analysis"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Virology"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Thailand"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3744434?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3744434"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23967358&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23967358"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0002349"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-02-28"],"dateOfCreation":["2013-08-22"],"electronicPublicationDate":["2013-08-15"],"firstPublicationDate":["2013-08-15"],"cprojectID":"PMC3744434"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSRJ9hGurOzZIXi","_score":1,"_source":{"id":["22305269"],"source":["MED"],"pmid":["22305269"],"pmcid":["PMC3310457"],"doi":["10.3201/eid1802.111224"],"title":["Zika virus infection, Cambodia, 2010."],"authorString":["Heang V, Yasuda CY, Sovann L, Haddow AD, Travassos da Rosa AP, Tesh RB, Kasper MR."],"authorList":[{"author":[{"fullName":["Heang V"],"firstName":["Vireak"],"lastName":["Heang"],"initials":["V"]},{"fullName":["Yasuda CY"],"firstName":["Chadwick Y"],"lastName":["Yasuda"],"initials":["CY"]},{"fullName":["Sovann L"],"firstName":["Ly"],"lastName":["Sovann"],"initials":["L"]},{"fullName":["Haddow AD"],"firstName":["Andrew D"],"lastName":["Haddow"],"initials":["AD"]},{"fullName":["Travassos da Rosa AP"],"firstName":["Amelia P"],"lastName":["Travassos da Rosa"],"initials":["AP"]},{"fullName":["Tesh RB"],"firstName":["Robert B"],"lastName":["Tesh"],"initials":["RB"]},{"fullName":["Kasper MR"],"firstName":["Matthew R"],"lastName":["Kasper"],"initials":["MR"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8957-2608","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["18"],"journalIssueId":["1902485"],"dateOfPublication":["2012 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2012"],"printPublicationDate":["2012-02-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2012"],"pageInfo":["349-351"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter","Case Reports"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Child, Preschool"]},{"majorTopic_YN":["N"],"descriptorName":["Cambodia"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Typing"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["DNA, Viral"],"registryNumber":["0"]},{"name":["Antibodies, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3310457?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3310457"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22305269&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22305269"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1802.111224"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["19"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2012-05-23"],"dateOfCreation":["2012-02-06"],"dateOfRevision":["2016-02-05"],"firstPublicationDate":["2012-02-01"],"cprojectID":"PMC3310457"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSaJ9hGurOzZIXu","_score":1,"_source":{"id":["24825977"],"source":["MED"],"pmid":["24825977"],"pmcid":["PMC3979174"],"title":["Magnetized solutions altered the absorbance of tramadol hcl: uv-spectrophotometer study."],"authorString":["Al-Nimer MS, Neda FSh, Lateef ZW."],"authorList":[{"author":[{"fullName":["Al-Nimer MS"],"firstName":["Marwan S M"],"lastName":["Al-Nimer"],"initials":["MS"],"affiliation":["Department of Pharmacology College of Medicine Al-Mustansiriya University P.O. Box 14132 Baghdad, Iraq."]},{"fullName":["Neda FSh"],"firstName":["Fezaa Sh"],"lastName":["Neda"],"initials":["FSh"],"affiliation":["Department of Physiology, College of Medicine, Al-Mustansiriya University, P.O. Box 14132, Baghdad, Iraq."]},{"fullName":["Lateef ZW"],"firstName":["Zainab W Abdul"],"lastName":["Lateef"],"initials":["ZW"],"affiliation":["Department of Physiology, College of Medicine, Al-Mustansiriya University, P.O. Box 14132, Baghdad, Iraq."]}]}],"journalInfo":[{"issue":["2"],"volume":["1"],"journalIssueId":["2159655"],"dateOfPublication":["2010 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2010"],"printPublicationDate":["2010-03-01"],"journal":[{"title":["Journal of basic and clinical pharmacy"],"ISOAbbreviation":["J Basic Clin Pharm"],"medlineAbbreviation":["J Basic Clin Pharm"],"NLMid":["101599515"],"ISSN":["0976-0105"],"ESSN":["0976-0113"]}]}],"pubYear":["2010"],"pageInfo":["119-123"],"abstractText":["Magnetized water showed alterations in the electrolyte potential and vibration modes. This study aimed to investigate the effect of magnetic water and physiological solutions on the UV-spectra of tramadol HCl. Distilled water as well as full strength or diluted physiological solutions are magnetized by magnetic disc. Then tramadol HCl was dissolved in each magnetic and in non-magnetic solution to obtain the following final concentrations: 0, 25, 50, 100, and 200μg/mL. The pH of each solution and the absorbance (O.D.) was recorded at wavelength 271 nm. Magnetized water or physiological solutions showed alterations in pH as well as in the absorbance (O.D.) of tramadol HCl detected by ultraviolet spectra at λ271 nm. It concludes that magnetized physiological solutions adversely altered the stability of tramadol HCl."],"affiliation":["Department of Pharmacology College of Medicine Al-Mustansiriya University P.O. Box 14132 Baghdad, Iraq."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Magnetized Solutions","Tramadol Hcl"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3979174?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3979174"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24825977"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24825977&action=stream&blobtype=pdf"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-05-14"],"dateOfCreation":["2014-05-14"],"dateOfRevision":["2014-05-23"],"electronicPublicationDate":["2010-05-15"],"firstPublicationDate":["2010-03-01"],"cprojectID":"PMC3979174"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSbhOUS2orL1IN2","_score":1,"_source":{"id":["23170174"],"source":["MED"],"pmid":["23170174"],"pmcid":["PMC3499821"],"doi":["10.3390/v4091592"],"title":["Filovirus research in Gabon and equatorial Africa: the experience of a research center in the heart of Africa."],"authorString":["Leroy E, Gonzalez JP."],"authorList":[{"author":[{"fullName":["Leroy E"],"firstName":["Eric"],"lastName":["Leroy"],"initials":["E"],"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}}]},{"fullName":["Gonzalez JP"],"firstName":["Jean Paul"],"lastName":["Gonzalez"],"initials":["JP"],"authorId":[{"_":"0000-0003-3063-1770","$":{"type":"ORCID"}}]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-0022-0890","$":{"type":"ORCID"}},{"_":"0000-0003-3063-1770","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["9"],"volume":["4"],"journalIssueId":["1985220"],"dateOfPublication":["2012 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2012"],"printPublicationDate":["2012-09-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2012"],"pageInfo":["1592-1604"],"abstractText":["Health research programs targeting the population of Gabon and Equatorial Africa at the International Center for Medical Research in Franceville (CIRMF), Gabon, have evolved during the years since its inception in 1979 in accordance with emerging diseases. Since the reemergence of Ebola virus in Central Africa, the CIRMF \"Emerging Viral Disease Unit\" developed diagnostic tools and epidemiologic strategies and transfers of such technology to support the response of the National Public Health System and the World Health Organization to epidemics of Ebola virus disease. The Unit carries out a unique investigation program on the natural history of the filoviruses, emergence of epidemics, and Ebola virus pathogenesis. In addition, academic training is provided at all levels to regional and international students covering emerging conditions (host factors, molecular biology, genetics) that favor the spread of viral diseases."],"affiliation":["Centre International de Recherches Médicales de Franceville (Franceville International Center for Medical Research), CIRMF, Libreville BP 2105, Gabon. eric.leroy@ird.fr"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Chiroptera"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Hemorrhagic Fever, Ebola"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Epidemiologic Methods"]},{"majorTopic_YN":["N"],"descriptorName":["Infection Control"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Biomedical Research"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]},{"abbreviation":["TD"],"qualifierName":["trends"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ebolavirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["Y"]}]}]}]}],"keywordList":[{"keyword":["Bats","Hemorrhagic fever","immunology","Gabon","Filovirus","Ebola","Central Africa","Cirmf"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3499821?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3499821"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23170174"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23170174&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v4091592"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-04-24"],"dateOfCreation":["2012-11-21"],"dateOfRevision":["2013-07-11"],"electronicPublicationDate":["2012-09-13"],"firstPublicationDate":["2012-09-13"],"cprojectID":"PMC3499821"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqUf_8VqgcuJwvf4","_score":1,"_source":{"id":["24294219"],"source":["MED"],"pmid":["24294219"],"pmcid":["PMC3827716"],"doi":["10.3389/fgene.2013.00240"],"title":["Practice variation across consent templates for biobank research. a survey of German biobanks."],"authorString":["Hirschberg I, Knüppel H, Strech D."],"authorList":[{"author":[{"fullName":["Hirschberg I"],"firstName":["Irene"],"lastName":["Hirschberg"],"initials":["I"],"affiliation":["Hannover Medical School, Institute for History, Ethics and Philosophy of Medicine / Centre for Ethics and Law in the Life Sciences Hannover, Germany."]},{"fullName":["Knüppel H"],"firstName":["Hannes"],"lastName":["Knüppel"],"initials":["H"]},{"fullName":["Strech D"],"firstName":["Daniel"],"lastName":["Strech"],"initials":["D"],"authorId":[{"_":"0000-0002-9153-079X","$":{"type":"ORCID"}}]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9153-079X","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2003505"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Frontiers in genetics"],"ISOAbbreviation":["Front Genet"],"medlineAbbreviation":["Front Genet"],"NLMid":["101560621"],"ESSN":["1664-8021"]}]}],"pubYear":["2013"],"pageInfo":["240"],"abstractText":["INTRODUCTION: Informed, voluntary, and valid consent from biomaterial donors is a precondition for biobank research. Valid consent protects donors' rights and helps maintain public trust in biobank research. Harmonization of consent procedures in biobank research is needed, because of the widely shared vision on national and international networking of biobanks including data and sample sharing. So far, no study has assessed and compared the content of current consent forms especially for biobank research. The objective of this study was to perform a content analysis of consent forms in German biobanks. METHODS: Based on ten guidelines for biomedical research, we developed an assessment matrix with 41 content issues that are potentially relevant for consent forms in biobank research. This assessment matrix was applied in a thematic text analysis to 30 consent documents of German biobanks identified via the German Biobank Registry in July 2012. RESULTS: Coverage of the 41 items in the assessed consent forms varied widely. For example, the items \"Right to withdraw consent (without disadvantage),\" \"Policy for genetic information/consent to genetic analyzes\" and \"International cooperation/transborder use\" were addressed in 97, 40, and 23% of all 30 consent forms respectively. The number of items covered by a single consent form ranged from 9 to 36 (22-88% out of 41 items). DISCUSSION: Our findings serve as a starting point to reflect upon the spectrum of consent issues that must be addressed in biobank research. The findings show that the majority of consent forms for German biobanks, if not all, should be improved and harmonized to better support an informed and balanced choice of potential donors and to facilitate networking of biobanks. Best practice models for consent forms in biobank research should be developed and biobank operators need to be more aware of relevant consent issues."],"affiliation":["Hannover Medical School, Institute for History, Ethics and Philosophy of Medicine / Centre for Ethics and Law in the Life Sciences Hannover, Germany."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Survey","Informed consent","Research Ethics","Biobank","Tissue Bank","Broad Consent","Thematic Text Analysis"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3827716?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3827716"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24294219&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24294219"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fgene.2013.00240"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-12-02"],"dateOfCreation":["2013-12-02"],"dateOfRevision":["2014-07-31"],"electronicPublicationDate":["2013-11-14"],"firstPublicationDate":["2013-11-14"],"cprojectID":"PMC3827716"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqST_8VqgcuJwvfr","_score":1,"_source":{"id":["2533555"],"source":["MED"],"pmid":["2533555"],"pmcid":["PMC1474587"],"doi":["10.1289/ehp.8983191"],"title":["Application of a membrane model to the sorptive interactions of humic substances."],"authorString":["Wershaw RL."],"authorList":[{"author":[{"fullName":["Wershaw RL"],"lastName":["Wershaw"],"initials":["RL"]}]}],"journalInfo":[{"volume":["83"],"journalIssueId":["722866"],"dateOfPublication":["1989 Nov"],"monthOfPublication":["11"],"yearOfPublication":["1989"],"printPublicationDate":["1989-11-01"],"journal":[{"title":["Environmental health perspectives"],"ISOAbbreviation":["Environ. Health Perspect."],"medlineAbbreviation":["Environ Health Perspect"],"NLMid":["0330411"],"ISSN":["0091-6765"],"ESSN":["1552-9924"]}]}],"pubYear":["1989"],"pageInfo":["191-203"],"abstractText":["Humic substances, the dark-colored, natural organic polyelectrolytes that are found in practically all soils, sediments, and natural water, strongly interact with both inorganic and organic pollutants. Inorganic cationic species generally undergo complexation reactions with humic substances. The binding of cations, such as cupric ions, by humic substances often markedly reduces their toxicity to aquatic organisms. Some inorganic anionic species, in the presence of metal ions, are sorbed by humic substances. In these instances the metal ions appear to form bridges between the humic substances and the anions. Several different types of interactions take place between organic compounds and humic materials. Hydrophobic organic species partition into either insoluble or soluble humic substances. The insoluble humic substances will remove hydrophobic organic compounds from the aqueous phase, thereby rendering them less mobile. However, soluble humic substances will solubilize hydrophobic organics, increasing their mobility. Other types of interactions between humic substances and organic compounds, such as adsorption and ion exchange, also have been observed. These various interactions between humic substances and pollutants are important in governing their fate and movement in natural water systems, and, for this reason, a detailed understanding of the mechanisms of the interaction is important. A recently developed membrane model of the structure of humic substances is described; this model enables one to better understand the physical-chemical properties of these materials."],"affiliation":["U.S. Geological Survey, Arveda, CO 80002."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Humic Substances"]},{"majorTopic_YN":["Y"],"descriptorName":["Soil Pollutants"]},{"majorTopic_YN":["N"],"descriptorName":["Water Movements"]},{"majorTopic_YN":["N"],"descriptorName":["Adsorption"]},{"majorTopic_YN":["Y"],"descriptorName":["Models, Molecular"]}]}],"chemicalList":[{"chemical":[{"name":["Humic Substances"],"registryNumber":["0"]},{"name":["Soil Pollutants"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1474587?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1474587"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1289/ehp.8983191"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["3"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["1990-03-16"],"dateOfCreation":["1990-03-16"],"dateOfRevision":["2008-11-20"],"firstPublicationDate":["1989-11-01"],"cprojectID":"PMC1474587"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr0JhOUS2orL1IR8","_score":1,"_source":{"id":["27344225"],"source":["MED"],"pmid":["27344225"],"pmcid":["PMC4972550"],"doi":["10.1016/j.ebiom.2016.06.009"],"title":["Assessing Seasonal Risks for the Introduction and Mosquito-borne Spread of Zika Virus in Europe."],"authorString":["Rocklöv J, Quam MB, Sudre B, German M, Kraemer MU, Brady O, Bogoch II, Liu-Helmersson J, Wilder-Smith A, Semenza JC, Ong M, Aaslav KK, Khan K."],"authorList":[{"author":[{"fullName":["Rocklöv J"],"firstName":["Joacim"],"lastName":["Rocklöv"],"initials":["J"],"affiliation":["Department of Public Health and Clinical Medicine, Epidemiology and Global Health, Umeå University, Sweden. Electronic address: joacim.rocklov@umu.se."]},{"fullName":["Quam MB"],"firstName":["Mikkel Brandon"],"lastName":["Quam"],"initials":["MB"],"affiliation":["Department of Public Health and Clinical Medicine, Epidemiology and Global Health, Umeå University, Sweden."]},{"fullName":["Sudre B"],"firstName":["Bertrand"],"lastName":["Sudre"],"initials":["B"],"affiliation":["European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden."]},{"fullName":["German M"],"firstName":["Matthew"],"lastName":["German"],"initials":["M"],"affiliation":["Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, ON, Canada."]},{"fullName":["Kraemer MU"],"firstName":["Moritz U G"],"lastName":["Kraemer"],"initials":["MU"],"affiliation":["Spatial Ecology and Epidemiology Group, Department of Zoology, University of Oxford; Oxford, UK."]},{"fullName":["Brady O"],"firstName":["Oliver"],"lastName":["Brady"],"initials":["O"],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford; Oxford, UK."]},{"fullName":["Bogoch II"],"firstName":["Isaac I"],"lastName":["Bogoch"],"initials":["II"],"affiliation":["Department of Medicine, Division of Infectious Diseases, University of Toronto, Toronto, ON, Canada; Divisions of Internal Medicine and Infectious Diseases, University Health Network, Toronto, Canada."]},{"fullName":["Liu-Helmersson J"],"firstName":["Jing"],"lastName":["Liu-Helmersson"],"initials":["J"],"affiliation":["Department of Public Health and Clinical Medicine, Epidemiology and Global Health, Umeå University, Sweden."]},{"fullName":["Wilder-Smith A"],"firstName":["Annelies"],"lastName":["Wilder-Smith"],"initials":["A"],"affiliation":["Department of Public Health and Clinical Medicine, Epidemiology and Global Health, Umeå University, Sweden; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore."]},{"fullName":["Semenza JC"],"firstName":["Jan C"],"lastName":["Semenza"],"initials":["JC"],"affiliation":["European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden."]},{"fullName":["Ong M"],"firstName":["Mark"],"lastName":["Ong"],"initials":["M"],"affiliation":["Geomatics Program, University of Waterloo, Waterloo, ON, Canada."]},{"fullName":["Aaslav KK"],"firstName":["Kaja Kaasik"],"lastName":["Aaslav"],"initials":["KK"],"affiliation":["European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden."]},{"fullName":["Khan K"],"firstName":["Kamran"],"lastName":["Khan"],"initials":["K"],"affiliation":["Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, ON, Canada; Department of Medicine, Division of Infectious Diseases, University of Toronto, Toronto, ON, Canada."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2435137"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["250-256"],"abstractText":["The explosive Zika virus epidemic in the Americas is amplifying spread of this emerging pathogen into previously unaffected regions of the world, including Europe (Gulland, 2016), where local populations are immunologically naïve. As summertime approaches in the northern hemisphere, Aedes mosquitoes in Europe may find suitable climatic conditions to acquire and subsequently transmit Zika virus from viremic travellers to local populations. While Aedes albopictus has proven to be a vector for the transmission of dengue and chikungunya viruses in Europe (Delisle et al., 2015; ECDC, n.d.) there is growing experimental and ecological evidence to suggest that it may also be competent for Zika virus(Chouin-Carneiro et al., 2016; Grard et al., 2014; Li et al., 2012; Wong et al., 2013). Here we analyze and overlay the monthly flows of airline travellers arriving into European cities from Zika affected areas across the Americas, the predicted monthly estimates of the basic reproduction number of Zika virus in areas where Aedes mosquito populations reside in Europe (Aedes aegypti in Madeira, Portugal and Ae. albopictus in continental Europe), and human populations living within areas where mosquito-borne transmission of Zika virus may be possible. We highlight specific geographic areas and timing of risk for Zika virus introduction and possible spread within Europe to inform the efficient use of human disease surveillance, vector surveillance and control, and public education resources."],"affiliation":["Department of Public Health and Clinical Medicine, Epidemiology and Global Health, Umeå University, Sweden. Electronic address: joacim.rocklov@umu.se."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","Climate","Air Travel","Zikv","Zika","Globalization, Mosquito"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972550?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972550"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.06.009"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-01"],"dateOfRevision":["2016-08-12"],"electronicPublicationDate":["2016-06-10"],"firstPublicationDate":["2016-06-10"],"cprojectID":"PMC4972550"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr1q_8VqgcuJwvj3","_score":1,"_source":{"id":["27457219"],"source":["MED"],"pmid":["27457219"],"pmcid":["PMC4960802"],"doi":["10.1186/s13059-016-1019-8"],"title":["Virus genomics and evolution: the transformative effect of new technologies and multidisciplinary collaboration on virus research and outbreak management."],"authorString":["Ip CL, Pybus OG, Gardy JL."],"authorList":[{"author":[{"fullName":["Ip CL"],"firstName":["Camilla L C"],"lastName":["Ip"],"initials":["CL"],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX37BN, UK."]},{"fullName":["Pybus OG"],"firstName":["Oliver G"],"lastName":["Pybus"],"initials":["OG"],"affiliation":["Department of Zoology, University of Oxford, Oxford, OX1 3PS, UK."]},{"fullName":["Gardy JL"],"firstName":["Jennifer L"],"lastName":["Gardy"],"initials":["JL"],"affiliation":["British Columbia Centre for Disease Control, Vancouver, BC, V5Z 4R4, Canada. jennifer.gardy@bccdc.ca."]}]}],"journalInfo":[{"issue":["1"],"volume":["17"],"journalIssueId":["2368932"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome biology"],"ISOAbbreviation":["Genome Biol."],"medlineAbbreviation":["Genome Biol"],"NLMid":["100960660"],"ISSN":["1474-7596"],"ESSN":["1474-760X"]}]}],"pubYear":["2016"],"pageInfo":["159"],"abstractText":["The first Virus Genomics and Evolution Conference was held at the Wellcome Genome Campus in Hinxton, UK, 8-10 June 2016."],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX37BN, UK."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960802?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960802"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13059-016-1019-8"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-26"],"dateOfRevision":["2016-07-29"],"electronicPublicationDate":["2016-07-25"],"firstPublicationDate":["2016-07-25"],"cprojectID":"PMC4960802"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr0mhOUS2orL1IR-","_score":1,"_source":{"id":["27489330"],"source":["MED"],"pmid":["27489330"],"pmcid":["PMC4973205"],"doi":["10.4269/ajtmh.16-0281"],"title":["Arboviruses: Molecular Biology, Evolution and Control. Nikos Vasilakis and Duane J. Gubler."],"authorString":["Blitvich B."],"authorList":[{"author":[{"fullName":["Blitvich B"],"firstName":["Bradley"],"lastName":["Blitvich"],"initials":["B"],"affiliation":["Iowa State University, 2116 Veterinary Medicine Building, Ames, IA 50011-1250. E-mail: blitvich@iastate.edu."]}]}],"journalInfo":[{"issue":["2"],"volume":["95"],"journalIssueId":["2437453"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2016"],"pageInfo":["488-489"],"affiliation":["Iowa State University, 2116 Veterinary Medicine Building, Ames, IA 50011-1250. E-mail: blitvich@iastate.edu."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973205?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973205"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.16-0281"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-08-24"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4973205"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3W_8VqgcuJwvj7","_score":1,"_source":{"id":["27467271"],"source":["MED"],"pmid":["27467271"],"pmcid":["PMC4965063"],"doi":["10.1371/journal.pbio.1002520"],"title":["Preventing Zika Virus Infection during Pregnancy Using a Seasonal Window of Opportunity for Conception."],"authorString":["Martinez ME."],"authorList":[{"author":[{"fullName":["Martinez ME"],"firstName":["Micaela Elvira"],"lastName":["Martinez"],"initials":["ME"],"authorId":[{"_":"0000-0002-9248-9450","$":{"type":"ORCID"}}],"affiliation":["Global Health Program, Woodrow Wilson School of Public and International Affairs, Princeton University, Princeton, New Jersey, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9248-9450","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["14"],"journalIssueId":["2424217"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["PLoS biology"],"ISOAbbreviation":["PLoS Biol."],"medlineAbbreviation":["PLoS Biol"],"NLMid":["101183755"],"ISSN":["1544-9173"],"ESSN":["1545-7885"]}]}],"pubYear":["2016"],"pageInfo":["e1002520"],"abstractText":["It has come to light that Zika virus (ZIKV) infection during pregnancy can result in trans-placental transmission to the fetus along with fetal death, congenital microcephaly, and/or Central Nervous System (CNS) malformations. There are projected to be >9,200,000 births annually in countries with ongoing ZIKV transmission. In response to the ZIKV threat, the World Health Organization (WHO) is strategically targeting prevention of infection in pregnant women and funding contraception in epidemic regions. I propose that the damaging effects of ZIKV can be reduced using a seasonal window of opportunity for conception that may minimize maternal exposure. Like other acute viral infections-including the related flavivirus, dengue virus (DENV)-the transmission of ZIKV is anticipated to be seasonal. By seasonally planning pregnancy, this aspect of pathogen ecology can be leveraged to align sensitive periods of gestation with the low-transmission season."],"affiliation":["Global Health Program, Woodrow Wilson School of Public and International Affairs, Princeton University, Princeton, New Jersey, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4965063?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4965063"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pbio.1002520"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-29"],"dateOfRevision":["2016-08-19"],"electronicPublicationDate":["2016-07-28"],"firstPublicationDate":["2016-07-28"],"cprojectID":"PMC4965063"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3ihOUS2orL1ISG","_score":1,"_source":{"id":["27500641"],"source":["MED"],"pmid":["27500641"],"pmcid":["PMC4976923"],"doi":["10.1371/journal.ppat.1005737"],"title":["Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling."],"authorString":["Lim SP, Noble CG, Seh CC, Soh TS, El Sahili A, Chan GK, Lescar J, Arora R, Benson T, Nilar S, Manjunatha U, Wan KF, Dong H, Xie X, Shi PY, Yokokawa F."],"authorList":[{"author":[{"fullName":["Lim SP"],"firstName":["Siew Pheng"],"lastName":["Lim"],"initials":["SP"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Noble CG"],"firstName":["Christian Guy"],"lastName":["Noble"],"initials":["CG"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Seh CC"],"firstName":["Cheah Chen"],"lastName":["Seh"],"initials":["CC"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Soh TS"],"firstName":["Tingjin Sherryl"],"lastName":["Soh"],"initials":["TS"],"affiliation":["School of Biological Sciences, Nanyang Technological University, Singapore."]},{"fullName":["El Sahili A"],"firstName":["Abbas"],"lastName":["El Sahili"],"initials":["A"],"affiliation":["School of Biological Sciences, Nanyang Technological University, Singapore."]},{"fullName":["Chan GK"],"firstName":["Grace Kar Yarn"],"lastName":["Chan"],"initials":["GK"],"affiliation":["School of Biological Sciences, Nanyang Technological University, Singapore."]},{"fullName":["Lescar J"],"firstName":["Julien"],"lastName":["Lescar"],"initials":["J"],"affiliation":["UPMC UMRS CR7-CNRS ERL 8255-INSERM U1135 Centre d'Immunologie et des Maladies Infectieuses, Centre Hospitalier Universitaire Pitié-Salpêtrière, Faculté de Médecine Pierre et Marie Curie, Paris, France."]},{"fullName":["Arora R"],"firstName":["Rishi"],"lastName":["Arora"],"initials":["R"],"affiliation":["Novartis Institute for Biomedical Research, Cambridge, Massachusetts, United States of America."]},{"fullName":["Benson T"],"firstName":["Timothy"],"lastName":["Benson"],"initials":["T"],"affiliation":["Novartis Institute for Biomedical Research, Cambridge, Massachusetts, United States of America."]},{"fullName":["Nilar S"],"firstName":["Shahul"],"lastName":["Nilar"],"initials":["S"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Manjunatha U"],"firstName":["Ujjini"],"lastName":["Manjunatha"],"initials":["U"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Wan KF"],"firstName":["Kah Fei"],"lastName":["Wan"],"initials":["KF"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Dong H"],"firstName":["Hongping"],"lastName":["Dong"],"initials":["H"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Xie X"],"firstName":["Xuping"],"lastName":["Xie"],"initials":["X"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Shi PY"],"firstName":["Pei-Yong"],"lastName":["Shi"],"initials":["PY"],"authorId":[{"_":"0000-0001-5553-1616","$":{"type":"ORCID"}}],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]},{"fullName":["Yokokawa F"],"firstName":["Fumiaki"],"lastName":["Yokokawa"],"initials":["F"],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-5553-1616","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["12"],"journalIssueId":["2436285"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005737"],"abstractText":["Flaviviruses comprise major emerging pathogens such as dengue virus (DENV) or Zika virus (ZIKV). The flavivirus RNA genome is replicated by the RNA-dependent-RNA polymerase (RdRp) domain of non-structural protein 5 (NS5). This essential enzymatic activity renders the RdRp attractive for antiviral therapy. NS5 synthesizes viral RNA via a \"de novo\" initiation mechanism. Crystal structures of the flavivirus RdRp revealed a \"closed\" conformation reminiscent of a pre-initiation state, with a well ordered priming loop that extrudes from the thumb subdomain into the dsRNA exit tunnel, close to the \"GDD\" active site. To-date, no allosteric pockets have been identified for the RdRp, and compound screening campaigns did not yield suitable drug candidates. Using fragment-based screening via X-ray crystallography, we found a fragment that bound to a pocket of the apo-DENV RdRp close to its active site (termed \"N pocket\"). Structure-guided improvements yielded DENV pan-serotype inhibitors of the RdRp de novo initiation activity with nano-molar potency that also impeded elongation activity at micro-molar concentrations. Inhibitors exhibited mixed inhibition kinetics with respect to competition with the RNA or GTP substrate. The best compounds have EC50 values of 1-2 μM against all four DENV serotypes in cell culture assays. Genome-sequencing of compound-resistant DENV replicons, identified amino acid changes that mapped to the N pocket. Since inhibitors bind at the thumb/palm interface of the RdRp, this class of compounds is proposed to hinder RdRp conformational changes during its transition from initiation to elongation. This is the first report of a class of pan-serotype and cell-active DENV RdRp inhibitors. Given the evolutionary conservation of residues lining the N pocket, these molecules offer insights to treat other serious conditions caused by flaviviruses."],"affiliation":["Novartis Institute for Tropical Diseases, Singapore."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976923?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976923"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005737"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["PDB"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-09"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-08"],"firstPublicationDate":["2016-08-08"],"cprojectID":"PMC4976923"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr4HJ9hGurOzZIb7","_score":1,"_source":{"id":["27509902"],"source":["MED"],"pmid":["27509902"],"pmcid":["PMC4979356"],"doi":["10.1136/bmj.i3899"],"title":["Congenital Zika syndrome with arthrogryposis: retrospective case series study."],"authorString":["van der Linden V, Filho EL, Lins OG, van der Linden A, Aragão Mde F, Brainer-Lima AM, Cruz DD, Rocha MA, Sobral da Silva PF, Carvalho MD, do Amaral FJ, Gomes JA, Ribeiro de Medeiros IC, Ventura CV, Ramos RC."],"authorList":[{"author":[{"fullName":["van der Linden V"],"firstName":["Vanessa"],"lastName":["van der Linden"],"initials":["V"],"affiliation":["Association for Assistance of Disabled Children, AACD, Recife, Brazil Barão de Lucena Hospital, HBL, Recife, Brazil vanessavdlinden@hotmail.com."]},{"fullName":["Filho EL"],"firstName":["Epitacio Leite Rolim"],"lastName":["Filho"],"initials":["EL"],"affiliation":["Association for Assistance of Disabled Children, AACD, Recife, Brazil Federal University of Pernambuco, UFPE, Recife, Brazil."]},{"fullName":["Lins OG"],"firstName":["Otavio Gomes"],"lastName":["Lins"],"initials":["OG"],"affiliation":["Federal University of Pernambuco, UFPE, Recife, Brazil."]},{"fullName":["van der Linden A"],"firstName":["Ana"],"lastName":["van der Linden"],"initials":["A"],"affiliation":["Prof Fernando Figueira Integral Medicine Institute, IMIP, Recife, Brazil vanessavdlinden@hotmail.com."]},{"fullName":["Aragão Mde F"],"firstName":["Maria de Fátima Viana Vasco"],"lastName":["Aragão"],"initials":["Mde F"],"affiliation":["Centro Diagnóstico Multimagem, Recife, Brazil Mauricio de Nassau University, Recife, Brazil."]},{"fullName":["Brainer-Lima AM"],"firstName":["Alessandra Mertens"],"lastName":["Brainer-Lima"],"initials":["AM"],"affiliation":["Mauricio de Nassau University, Recife, Brazil University of Pernambuco, UPE, Recife, Brazil."]},{"fullName":["Cruz DD"],"firstName":["Danielle Di Cavalcanti Sousa"],"lastName":["Cruz"],"initials":["DD"],"affiliation":["Prof Fernando Figueira Integral Medicine Institute, IMIP, Recife, Brazil."]},{"fullName":["Rocha MA"],"firstName":["Maria Angela Wanderley"],"lastName":["Rocha"],"initials":["MA"],"affiliation":["Oswaldo Cruz University Hospital, HUOC, Recife, Brazil."]},{"fullName":["Sobral da Silva PF"],"firstName":["Paula Fabiana"],"lastName":["Sobral da Silva"],"initials":["PF"],"affiliation":["Oswaldo Cruz University Hospital, HUOC, Recife, Brazil."]},{"fullName":["Carvalho MD"],"firstName":["Maria Durce Costa Gomes"],"lastName":["Carvalho"],"initials":["MD"],"affiliation":["Oswaldo Cruz University Hospital, HUOC, Recife, Brazil."]},{"fullName":["do Amaral FJ"],"firstName":["Fernando José"],"lastName":["do Amaral"],"initials":["FJ"],"affiliation":["Barão de Lucena Hospital, HBL, Recife, Brazil."]},{"fullName":["Gomes JA"],"firstName":["Joelma Arruda"],"lastName":["Gomes"],"initials":["JA"],"affiliation":["Barão de Lucena Hospital, HBL, Recife, Brazil."]},{"fullName":["Ribeiro de Medeiros IC"],"firstName":["Igor Colaço"],"lastName":["Ribeiro de Medeiros"],"initials":["IC"],"affiliation":["Hospital Infantil Jorge de Medeiros Recife, Brazil."]},{"fullName":["Ventura CV"],"firstName":["Camila V"],"lastName":["Ventura"],"initials":["CV"],"affiliation":["Altino Ventura Foundation, FAV, Recife, Brazil Pernambuco's Eye Hospital, Recife, Brazil."]},{"fullName":["Ramos RC"],"firstName":["Regina Coeli"],"lastName":["Ramos"],"initials":["RC"],"affiliation":["Oswaldo Cruz University Hospital, HUOC, Recife, Brazil."]}]}],"journalInfo":[{"volume":["354"],"journalIssueId":["2424732"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMJ (Clinical research ed.)"],"ISOAbbreviation":["BMJ"],"medlineAbbreviation":["BMJ"],"NLMid":["8900488"],"ISSN":["0959-8138"],"ESSN":["1756-1833"]}]}],"pubYear":["2016"],"pageInfo":["i3899"],"abstractText":["To describe the clinical, radiological, and electromyographic features in a series of children with joint contractures (arthrogryposis) associated with congenital infection presumably caused by Zika virus.Retrospective case series study.Association for Assistance of Disabled Children, Pernambuco state, Brazil.Seven children with arthrogryposis and a diagnosis of congenital infection presumably caused by Zika virus during the Brazilian microcephaly epidemic.Main clinical, radiological, and electromyographic findings, and likely correlation between clinical and primary neurological abnormalities.The brain images of all seven children were characteristic of congenital infection and arthrogryposis. Two children tested positive for IgM to Zika virus in the cerebrospinal fluid. Arthrogryposis was present in the arms and legs of six children (86%) and the legs of one child (14%). Hip radiographs showed bilateral dislocation in seven children, subluxation of the knee associated with genu valgus in three children (43%), which was bilateral in two (29%). All the children underwent high definition ultrasonography of the joints, and there was no evidence of abnormalities. Moderate signs of remodeling of the motor units and a reduced recruitment pattern were found on needle electromyography (monopolar). Five of the children underwent brain computed tomography (CT) and magnetic resonance imaging (MRI) and the remaining two CT only. All presented malformations of cortical development, calcifications predominantly in the cortex and subcortical white matter (especially in the junction between the cortex and white matter), reduction in brain volume, ventriculomegaly, and hypoplasia of the brainstem and cerebellum. MRI of the spine in four children showed apparent thinning of the cord and reduced ventral roots.Congenital Zika syndrome should be added to the differential diagnosis of congenital infections and arthrogryposis. The arthrogryposis was unrelated to the abnormalities of the joints themselves, but was possibly of neurogenic origin, with chronic involvement of central and peripheral motor neurones leading to deformities as a result of fixed postures in utero. Based on the neurophysiological observations, we suggest two possible mechanisms: tropism of neurones, with involvement of peripheral and central motor neurones, or a relation with vascular disorders."],"affiliation":["Association for Assistance of Disabled Children, AACD, Recife, Brazil Barão de Lucena Hospital, HBL, Recife, Brazil vanessavdlinden@hotmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4979356"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1136/bmj.i3899"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-11"],"dateOfRevision":["2016-08-20"],"electronicPublicationDate":["2016-08-09"],"firstPublicationDate":["2016-08-09"],"cprojectID":"PMC4979356"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr5Q_8VqgcuJwvkA","_score":1,"_source":{"id":["27489798"],"source":["MED"],"pmid":["27489798"],"pmcid":["PMC4969283"],"doi":["10.7774/cevr.2016.5.2.89"],"title":["Prospects for dengue vaccines for travelers."],"authorString":["Lim SK, Lee YS, Namkung S, Lim JK, Yoon IK."],"authorList":[{"author":[{"fullName":["Lim SK"],"firstName":["Sl-Ki"],"lastName":["Lim"],"initials":["SK"],"authorId":[{"_":"0000-0003-2246-6824","$":{"type":"ORCID"}}],"affiliation":["International Vaccine Institute, Seoul, Korea."]},{"fullName":["Lee YS"],"firstName":["Yong Seok"],"lastName":["Lee"],"initials":["YS"],"authorId":[{"_":"0000-0002-9633-654X","$":{"type":"ORCID"}}],"affiliation":["International Vaccine Institute, Seoul, Korea."]},{"fullName":["Namkung S"],"firstName":["Suk"],"lastName":["Namkung"],"initials":["S"],"authorId":[{"_":"0000-0002-2220-6785","$":{"type":"ORCID"}}],"affiliation":["International Vaccine Institute, Seoul, Korea."]},{"fullName":["Lim JK"],"firstName":["Jacqueline K"],"lastName":["Lim"],"initials":["JK"],"authorId":[{"_":"0000-0001-6744-7825","$":{"type":"ORCID"}}],"affiliation":["International Vaccine Institute, Seoul, Korea."]},{"fullName":["Yoon IK"],"firstName":["In-Kyu"],"lastName":["Yoon"],"initials":["IK"],"authorId":[{"_":"0000-0001-5077-6470","$":{"type":"ORCID"}}],"affiliation":["International Vaccine Institute, Seoul, Korea."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2246-6824","$":{"type":"ORCID"}},{"_":"0000-0002-9633-654X","$":{"type":"ORCID"}},{"_":"0000-0002-2220-6785","$":{"type":"ORCID"}},{"_":"0000-0001-6744-7825","$":{"type":"ORCID"}},{"_":"0000-0001-5077-6470","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["5"],"journalIssueId":["2436870"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Clinical and experimental vaccine research"],"ISOAbbreviation":["Clin Exp Vaccine Res"],"medlineAbbreviation":["Clin Exp Vaccine Res"],"NLMid":["101592344"],"ISSN":["2287-3651"],"ESSN":["2287-366X"]}]}],"pubYear":["2016"],"pageInfo":["89-100"],"abstractText":["Travel-acquired dengue cases have been increasing as the overall global dengue burden has expanded. In Korea, imported dengue cases have been reported since 2000 when it first became a notifiable disease. During the first four months of 2016, three times more dengue cases were reported in Korea than during the same period the previous year. A safe and efficacious vaccine for travelers would be beneficial to prevent dengue disease in individual travelers and potentially decrease the risk of virus spread to non-endemic areas. Here, we summarize the characteristics of dengue vaccines for travelers and review dengue vaccines currently licensed or in clinical development."],"affiliation":["International Vaccine Institute, Seoul, Korea."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Dengue","Travel","Dengue Vaccines"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969283?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969283"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7774/cevr.2016.5.2.89"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-04"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-08-07"],"electronicPublicationDate":["2016-07-29"],"firstPublicationDate":["2016-07-29"],"cprojectID":"PMC4969283"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr56hOUS2orL1ISQ","_score":1,"_source":{"id":["27191483"],"source":["MED"],"pmid":["27191483"],"pmcid":["PMC4982148"],"doi":["10.3201/eid2208.160270"],"title":["Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses."],"authorString":["Kilianski A, Roth PA, Liem AT, Hill JM, Willis KL, Rossmaier RD, Marinich AV, Maughan MN, Karavis MA, Kuhn JH, Honko AN, Rosenzweig CN."],"authorList":[{"author":[{"fullName":["Kilianski A"],"firstName":["Andy"],"lastName":["Kilianski"],"initials":["A"]},{"fullName":["Roth PA"],"firstName":["Pierce A"],"lastName":["Roth"],"initials":["PA"]},{"fullName":["Liem AT"],"firstName":["Alvin T"],"lastName":["Liem"],"initials":["AT"]},{"fullName":["Hill JM"],"firstName":["Jessica M"],"lastName":["Hill"],"initials":["JM"]},{"fullName":["Willis KL"],"firstName":["Kristen L"],"lastName":["Willis"],"initials":["KL"]},{"fullName":["Rossmaier RD"],"firstName":["Rebecca D"],"lastName":["Rossmaier"],"initials":["RD"]},{"fullName":["Marinich AV"],"firstName":["Andrew V"],"lastName":["Marinich"],"initials":["AV"]},{"fullName":["Maughan MN"],"firstName":["Michele N"],"lastName":["Maughan"],"initials":["MN"]},{"fullName":["Karavis MA"],"firstName":["Mark A"],"lastName":["Karavis"],"initials":["MA"]},{"fullName":["Kuhn JH"],"firstName":["Jens H"],"lastName":["Kuhn"],"initials":["JH"]},{"fullName":["Honko AN"],"firstName":["Anna N"],"lastName":["Honko"],"initials":["AN"]},{"fullName":["Rosenzweig CN"],"firstName":["C Nicole"],"lastName":["Rosenzweig"],"initials":["CN"]}]}],"journalInfo":[{"issue":["8"],"volume":["22"],"journalIssueId":["2403977"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1448-1451"],"abstractText":["Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Viruses","Sequencing","Genomics","RNA virus","Ebola Virus","Venezuelan Equine Encephalitis Virus","Nanopore Sequencing","Zika Virus","Fieldable Platform"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982148?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982148"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2208.160270"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-07-20"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-08-15"],"firstPublicationDate":["2016-08-15"],"cprojectID":"PMC4982148"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr6VJ9hGurOzZIcE","_score":1,"_source":{"id":["27525157"],"source":["MED"],"pmid":["27525157"],"pmcid":["PMC4981576"],"doi":["10.4258/hir.2016.22.3.164"],"title":["New Evaluation Vector through the Stanford Mobile Inquiry-Based Learning Environment (SMILE) for Participatory Action Research."],"authorString":["Kim P, An JY."],"authorList":[{"author":[{"fullName":["Kim P"],"firstName":["Paul"],"lastName":["Kim"],"initials":["P"],"affiliation":["Graduate School of Education, Stanford University, Stanford, CA, USA."]},{"fullName":["An JY"],"firstName":["Ji-Young"],"lastName":["An"],"initials":["JY"],"affiliation":["Graduate School of Education, Stanford University, Stanford, CA, USA.; u-Healthcare Design, Design Institute, Inje University, Seoul, Korea."]}]}],"journalInfo":[{"issue":["3"],"volume":["22"],"journalIssueId":["2440892"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Healthcare informatics research"],"ISOAbbreviation":["Healthc Inform Res"],"medlineAbbreviation":["Healthc Inform Res"],"NLMid":["101534553"],"ISSN":["2093-3681"],"ESSN":["2093-369X"]}]}],"pubYear":["2016"],"pageInfo":["164-171"],"abstractText":["This article reviews an evaluation vector model driven from a participatory action research leveraging a collective inquiry system named SMILE (Stanford Mobile Inquiry-based Learning Environment).SMILE has been implemented in a diverse set of collective inquiry generation and analysis scenarios including community health care-specific professional development sessions and community-based participatory action research projects. In each scenario, participants are given opportunities to construct inquiries around physical and emotional health-related phenomena in their own community.Participants formulated inquiries as well as potential clinical treatments and hypothetical scenarios to address health concerns or clarify misunderstandings or misdiagnoses often found in their community practices. From medical universities to rural village health promotion organizations, all participatory inquiries and potential solutions can be collected and analyzed. The inquiry and solution sets represent an evaluation vector which helps educators better understand community health issues at a much deeper level.SMILE helps collect problems that are most important and central to their community health concerns. The evaluation vector, consisting participatory and collective inquiries and potential solutions, helps the researchers assess the participants' level of understanding on issues around health concerns and practices while helping the community adequately formulate follow-up action plans. The method used in SMILE requires much further enhancement with machine learning and advanced data visualization."],"affiliation":["Graduate School of Education, Stanford University, Stanford, CA, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Public Health","Telemedicine","Social Learning","Community-based Participatory Research","Public Health Informatics"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981576?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981576"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4258/hir.2016.22.3.164"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-15"],"dateOfCreation":["2016-08-15"],"dateOfRevision":["2016-08-17"],"electronicPublicationDate":["2016-07-31"],"firstPublicationDate":["2016-07-31"],"cprojectID":"PMC4981576"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr69hOUS2orL1ISU","_score":1,"_source":{"id":["27529422"],"source":["MED"],"pmid":["27529422"],"pmcid":["PMC4987038"],"doi":["10.1371/journal.pmed.1002109"],"title":["Make Data Sharing Routine to Prepare for Public Health Emergencies."],"authorString":["Chretien JP, Rivers CM, Johansson MA."],"authorList":[{"author":[{"fullName":["Chretien JP"],"firstName":["Jean-Paul"],"lastName":["Chretien"],"initials":["JP"],"authorId":[{"_":"0000-0001-8143-6823","$":{"type":"ORCID"}}],"affiliation":["Integrated Biosurveillance Section, Armed Forces Health Surveillance Branch, Defense Health Agency, Silver Spring, Maryland, United States of America."]},{"fullName":["Rivers CM"],"firstName":["Caitlin M"],"lastName":["Rivers"],"initials":["CM"],"affiliation":["Epidemiology and Disease Surveillance, US Army Institute of Public Health, Aberdeen Proving Ground, Maryland, United States of America."]},{"fullName":["Johansson MA"],"firstName":["Michael A"],"lastName":["Johansson"],"initials":["MA"],"affiliation":["Dengue Branch, Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, San Juan, Puerto Rico, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-8143-6823","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["13"],"journalIssueId":["2436284"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS medicine"],"ISOAbbreviation":["PLoS Med."],"medlineAbbreviation":["PLoS Med"],"NLMid":["101231360"],"ISSN":["1549-1277"],"ESSN":["1549-1676"]}]}],"pubYear":["2016"],"pageInfo":["e1002109"],"abstractText":["Jean-Paul Chretien and colleagues argue that recent Ebola and Zika virus outbreaks highlight the importance of data sharing in scientific research."],"affiliation":["Integrated Biosurveillance Section, Armed Forces Health Surveillance Branch, Defense Health Agency, Silver Spring, Maryland, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4987038?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4987038"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pmed.1002109"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-17"],"dateOfRevision":["2016-08-31"],"electronicPublicationDate":["2016-08-16"],"firstPublicationDate":["2016-08-16"],"cprojectID":"PMC4987038"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr7LJ9hGurOzZIcH","_score":1,"_source":{"id":["27532496"],"source":["MED"],"pmid":["27532496"],"pmcid":["PMC4988691"],"doi":["10.1371/journal.pone.0161365"],"title":["Modeling Mosquito-Borne Disease Spread in U.S. Urbanized Areas: The Case of Dengue in Miami."],"authorString":["Robert MA, Christofferson RC, Silva NJ, Vasquez C, Mores CN, Wearing HJ."],"authorList":[{"author":[{"fullName":["Robert MA"],"firstName":["Michael A"],"lastName":["Robert"],"initials":["MA"],"affiliation":["Department of Mathematics and Statistics, University of New Mexico, Albuquerque, NM, United States of America."]},{"fullName":["Christofferson RC"],"firstName":["Rebecca C"],"lastName":["Christofferson"],"initials":["RC"],"affiliation":["Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA, United States of America."]},{"fullName":["Silva NJ"],"firstName":["Noah J B"],"lastName":["Silva"],"initials":["NJ"],"affiliation":["Department of Biology, University of New Mexico, Albuquerque, NM, United States of America."]},{"fullName":["Vasquez C"],"firstName":["Chalmers"],"lastName":["Vasquez"],"initials":["C"],"affiliation":["Miami-Dade County Mosquito Control Division, Miami, FL, United States of America."]},{"fullName":["Mores CN"],"firstName":["Christopher N"],"lastName":["Mores"],"initials":["CN"],"affiliation":["Department of Pathobiological Sciences, Louisiana State University, Baton Rouge, LA, United States of America."]},{"fullName":["Wearing HJ"],"firstName":["Helen J"],"lastName":["Wearing"],"initials":["HJ"],"affiliation":["Department of Mathematics and Statistics, University of New Mexico, Albuquerque, NM, United States of America."]}]}],"journalInfo":[{"issue":["8"],"volume":["11"],"journalIssueId":["2435494"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0161365"],"abstractText":["Expansion of mosquito-borne pathogens into more temperate regions of the world necessitates tools such as mathematical models for understanding the factors that contribute to the introduction and emergence of a disease in populations naïve to the disease. Often, these models are not developed and analyzed until after a pathogen is detected in a population. In this study, we develop a spatially explicit stochastic model parameterized with publicly available U.S. Census data for studying the potential for disease spread in Urbanized Areas of the United States. To illustrate the utility of the model, we specifically study the potential for introductions of dengue to lead to autochthonous transmission and outbreaks in a population representative of the Miami Urbanized Area, where introductions of dengue have occurred frequently in recent years. We describe seasonal fluctuations in mosquito populations by fitting a population model to trap data provided by the Miami-Dade Mosquito Control Division. We show that the timing and location of introduced cases could play an important role in determining both the probability that local transmission occurs as well as the total number of cases throughout the entire region following introduction. We show that at low rates of clinical presentation, small outbreaks of dengue could go completely undetected during a season, which may confound mitigation efforts that rely upon detection. We discuss the sensitivity of the model to several critical parameter values that are currently poorly characterized and motivate the collection of additional data to strengthen the predictive power of this and similar models. Finally, we emphasize the utility of the general structure of this model in studying mosquito-borne diseases such as chikungunya and Zika virus in other regions."],"affiliation":["Department of Mathematics and Statistics, University of New Mexico, Albuquerque, NM, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988691?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988691"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0161365"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-18"],"dateOfRevision":["2016-08-31"],"electronicPublicationDate":["2016-08-17"],"firstPublicationDate":["2016-08-17"],"cprojectID":"PMC4988691"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr63_8VqgcuJwvkD","_score":1,"_source":{"id":["27547128"],"source":["MED"],"pmid":["27547128"],"pmcid":["PMC4969322"],"title":["Zika Virus: Where Is the Treatment?"],"authorString":["Mumtaz N, van Kampen JJ, Reusken CB, Boucher CA, Koopmans MP."],"authorList":[{"author":[{"fullName":["Mumtaz N"],"firstName":["Noreen"],"lastName":["Mumtaz"],"initials":["N"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."]},{"fullName":["van Kampen JJ"],"firstName":["Jeroen J A"],"lastName":["van Kampen"],"initials":["JJ"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."]},{"fullName":["Reusken CB"],"firstName":["Chantal B E M"],"lastName":["Reusken"],"initials":["CB"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."]},{"fullName":["Boucher CA"],"firstName":["Charles A B"],"lastName":["Boucher"],"initials":["CA"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."]},{"fullName":["Koopmans MP"],"firstName":["Marion P G"],"lastName":["Koopmans"],"initials":["MP"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2443214"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Current treatment options in infectious diseases"],"ISOAbbreviation":["Curr Treat Options Infect Dis"],"medlineAbbreviation":["Curr Treat Options Infect Dis"],"NLMid":["100888980"],"ISSN":["1523-3820"],"ESSN":["1534-6250"]}]}],"pubYear":["2016"],"pageInfo":["208-211"],"affiliation":["Department of Viroscience, Unit Clinical Virology, Erasmus MC, Room Na-1018, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969322?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969322"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-22"],"dateOfRevision":["2016-08-22"],"electronicPublicationDate":["2016-07-08"],"firstPublicationDate":["2016-07-08"],"cprojectID":"PMC4969322"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8chOUS2orL1ISZ","_score":1,"_source":{"id":["PMC4991975"],"source":["PMC"],"pmcid":["PMC4991975"],"title":["Microbial enzymes: industrial progress in 21st century."],"authorString":["Singh R, Kumar M, Mittal A, Mehta PK."],"authorList":[{"author":[{"fullName":["Singh R"],"firstName":["Rajendra"],"lastName":["Singh"],"initials":["R"],"affiliation":["1Department of Biochemistry, VP Chest Institute, University of Delhi, Delhi, 110007 India"]},{"fullName":["Kumar M"],"firstName":["Manoj"],"lastName":["Kumar"],"initials":["M"]},{"fullName":["Mittal A"],"firstName":["Anshumali"],"lastName":["Mittal"],"initials":["A"]},{"fullName":["Mehta PK"],"firstName":["Praveen Kumar"],"lastName":["Mehta"],"initials":["PK"]}]}],"journalInfo":[{"issue":["2"],"volume":["6"],"journalIssueId":["2420148"],"dateOfPublication":["2016 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2016"],"printPublicationDate":["2016-12-01"],"journal":[{"title":["3 Biotech"],"ISOAbbreviation":["3 Biotech"],"medlineAbbreviation":["3 Biotech"],"NLMid":["101565857"],"ESSN":["2190-5738"]}]}],"pubYear":["2016"],"abstractText":["AbstractBiocatalytic potential of microorganisms have been employed for centuries to produce bread, wine, vinegar and other common products without understanding the biochemical basis of their ingredients. Microbial enzymes have gained interest for their widespread uses in industries and medicine owing to their stability, catalytic activity, and ease of production and optimization than plant and animal enzymes. The use of enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics. Microbial enzymes are capable of degrading toxic chemical compounds of industrial and domestic wastes (phenolic compounds, nitriles, amines etc.) either via degradation or conversion. Here in this review, we highlight and discuss current technical and scientific involvement of microorganisms in enzyme production and their present status in worldwide enzyme market.Graphical abstract"],"affiliation":["1Department of Biochemistry, VP Chest Institute, University of Delhi, Delhi, 110007 India"],"language":["eng"],"pubModel":["Undetermined"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Enzymes","Microorganisms","Application","Bioconversion","Industry"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991975"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.org/articles/PMC4991975/?tool=EBI"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-24"],"dateOfRevision":["2016-08-24"],"electronicPublicationDate":["2016-08-19"],"firstPublicationDate":["2016-08-19"],"cprojectID":"PMC4991975"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr88J9hGurOzZIcN","_score":1,"_source":{"id":["27347853"],"source":["MED"],"pmid":["27347853"],"pmcid":["PMC4994358"],"doi":["10.3201/eid2209.160725"],"title":["Specificity of Dengue NS1 Antigen in Differential Diagnosis of Dengue and Zika Virus Infection."],"authorString":["Matheus S, Boukhari R, Labeau B, Ernault V, Bremand L, Kazanji M, Rousset D."],"authorList":[{"author":[{"fullName":["Matheus S"],"firstName":["Séverine"],"lastName":["Matheus"],"initials":["S"]},{"fullName":["Boukhari R"],"firstName":["Rachida"],"lastName":["Boukhari"],"initials":["R"]},{"fullName":["Labeau B"],"firstName":["Bhety"],"lastName":["Labeau"],"initials":["B"]},{"fullName":["Ernault V"],"firstName":["Valérie"],"lastName":["Ernault"],"initials":["V"]},{"fullName":["Bremand L"],"firstName":["Laetitia"],"lastName":["Bremand"],"initials":["L"]},{"fullName":["Kazanji M"],"firstName":["Mirdad"],"lastName":["Kazanji"],"initials":["M"]},{"fullName":["Rousset D"],"firstName":["Dominique"],"lastName":["Rousset"],"initials":["D"]}]}],"journalInfo":[{"issue":["9"],"volume":["22"],"journalIssueId":["2409892"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1691-1693"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Specificity","NS1 protein","Differential diagnosis","Nonstructural protein","Dengue","cross-reactivity","Ns1 Antigen","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994358?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994358"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2209.160725"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-17"],"dateOfRevision":["2016-09-08"],"electronicPublicationDate":["2016-09-15"],"firstPublicationDate":["2016-09-15"],"cprojectID":"PMC4994358"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr9Q_8VqgcuJwvkL","_score":1,"_source":{"id":["26884455"],"source":["MED"],"pmid":["26884455"],"pmcid":["PMC4973165"],"doi":["10.4269/ajtmh.16-0096"],"title":["Zika Virus Infection Presenting with Postauricular Lymphadenopathy."],"authorString":["Weitzel T, Cortes CP."],"authorList":[{"author":[{"fullName":["Weitzel T"],"firstName":["Thomas"],"lastName":["Weitzel"],"initials":["T"],"affiliation":["Programa Medicina del Viajero, Clínica Alemana de Santiago, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago, Chile. Laboratorio Clínico, Clínica Alemana de Santiago, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago, Chile. thomas.weitzel@gmail.com."]},{"fullName":["Cortes CP"],"firstName":["Claudia P"],"lastName":["Cortes"],"initials":["CP"],"affiliation":["Departamento de Infectología, Clínica Santa María, Santiago, Chile. Departamento de Medicina, Escuela de Medicina, Universidad de Chile, Santiago, Chile."]}]}],"journalInfo":[{"issue":["2"],"volume":["95"],"journalIssueId":["2437453"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2016"],"pageInfo":["255-256"],"affiliation":["Programa Medicina del Viajero, Clínica Alemana de Santiago, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago, Chile. Laboratorio Clínico, Clínica Alemana de Santiago, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago, Chile. thomas.weitzel@gmail.com."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973165?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973165"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.16-0096"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-08-24"],"electronicPublicationDate":["2016-02-16"],"firstPublicationDate":["2016-02-16"],"cprojectID":"PMC4973165"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDbhOUS2orL1ISk","_score":1,"_source":{"id":["PMC5007542"],"source":["PMC"],"pmcid":["PMC5007542"],"title":["Zika virus: A call to action for physicians in the era of climate change."],"authorString":["Yang YT, Sarfaty M."],"authorList":[{"author":[{"fullName":["Yang YT"],"firstName":["Y. Tony"],"lastName":["Yang"],"initials":["YT"],"affiliation":["aDepartment of Health Administration and Policy, George Mason University, Fairfax, VA 22030, USA"]},{"fullName":["Sarfaty M"],"firstName":["Mona"],"lastName":["Sarfaty"],"initials":["M"]}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2429895"],"dateOfPublication":["2016 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2016"],"printPublicationDate":["2016-12-01"],"journal":[{"title":["Preventive Medicine Reports"],"medlineAbbreviation":["Preventive Medicine Reports"],"ISSN":["2211-3355"]}]}],"pubYear":["2016"],"pageInfo":["444-446"],"abstractText":["In February 2016, the World Health Organization declared the mosquito-borne Zika virus to be a “public health emergency of international concern” as the disease linked to thousands of birth defects in Brazil spreads rapidly. The distribution of the Aedes mosquitos has drastically increased over the past few decades, which have been the hottest decades on Earth in more than 1000 years based on climate proxy measures. Although a combination of factors explains the current Zika virus outbreak, it's highly likely that the changes in the climate contribute to the spread of Aedes vector carrying the Zika virus, the pathogen causing serious birth defects. Physicians, both individually and collectively, as trusted and educated members of society have critical roles to play. In addition to clinical management and prevention of Zika, physicians should communicate about the health benefits of addressing climate change in straightforward evidence-based language to their local communities and policymakers, and make clear their support for policies mitigating climate change."],"affiliation":["aDepartment of Health Administration and Policy, George Mason University, Fairfax, VA 22030, USA"],"language":["eng"],"pubModel":["Undetermined"],"pubTypeList":[{"pubType":["Editorial"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5007542"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.org/articles/PMC5007542/?tool=EBI"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-11"],"dateOfRevision":["2016-09-11"],"electronicPublicationDate":["2016-08-12"],"firstPublicationDate":["2016-08-12"],"cprojectID":"PMC5007542"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDghOUS2orL1ISm","_score":1,"_source":{"id":["27604081"],"source":["MED"],"pmid":["27604081"],"pmcid":["PMC5015245"],"doi":["10.1186/s12916-016-0686-3"],"title":["Infectious diseases epidemic threats and mass gatherings: refocusing global attention on the continuing spread of the Middle East Respiratory syndrome coronavirus (MERS-CoV)."],"authorString":["Zumla A, Alagaili AN, Cotten M, Azhar EI."],"authorList":[{"author":[{"fullName":["Zumla A"],"firstName":["Alimuddin"],"lastName":["Zumla"],"initials":["A"],"affiliation":["NIHR Biomedical Research Centre, UCL Hospitals NHS Foundation Trust, London, UK. a.zumla@ucl.ac.uk."]},{"fullName":["Alagaili AN"],"firstName":["Abdulaziz N"],"lastName":["Alagaili"],"initials":["AN"],"affiliation":["KSU Mammals Research Chair, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia."]},{"fullName":["Cotten M"],"firstName":["Matthew"],"lastName":["Cotten"],"initials":["M"],"affiliation":["Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands."]},{"fullName":["Azhar EI"],"firstName":["Esam I"],"lastName":["Azhar"],"initials":["EI"],"affiliation":["Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia."]}]}],"journalInfo":[{"issue":["1"],"volume":["14"],"journalIssueId":["2358711"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC medicine"],"ISOAbbreviation":["BMC Med"],"medlineAbbreviation":["BMC Med"],"NLMid":["101190723"],"ESSN":["1741-7015"]}]}],"pubYear":["2016"],"pageInfo":["132"],"abstractText":["Media and World Health Organization (WHO) attention on Zika virus transmission at the 2016 Rio Olympic Games and the 2015 Ebola virus outbreak in West Africa diverted the attention of global public health authorities from other lethal infectious diseases with epidemic potential. Mass gatherings such as the annual Hajj pilgrimage hosted by Kingdom of Saudi Arabia attract huge crowds from all continents, creating high-risk conditions for the rapid global spread of infectious diseases. The highly lethal Middle Eastern respiratory syndrome coronavirus (MERS-CoV) remains in the WHO list of top emerging diseases likely to cause major epidemics. The 2015 MERS-CoV outbreak in South Korea, in which 184 MERS cases including 33 deaths occurred in 2 months, that was imported from the Middle East by a South Korean businessman was a wake-up call for the global community to refocus attention on MERS-CoV and other emerging and re-emerging infectious diseases with epidemic potential. The international donor community and Middle Eastern countries should make available resources for, and make a serious commitment to, taking forward a \"One Health\" global network for proactive surveillance, rapid detection, and prevention of MERS-CoV and other epidemic infectious diseases threats."],"affiliation":["NIHR Biomedical Research Centre, UCL Hospitals NHS Foundation Trust, London, UK. a.zumla@ucl.ac.uk."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"keywordList":[{"keyword":["Infectious diseases","Control","Hajj","Mass Gathering","Mers-cov","Epidemic Transmission"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5015245?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5015245"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12916-016-0686-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-08"],"electronicPublicationDate":["2016-09-07"],"firstPublicationDate":["2016-09-07"],"cprojectID":"PMC5015245"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsEl_8VqgcuJwvki","_score":1,"_source":{"id":["27487998"],"source":["MED"],"pmid":["27487998"],"pmcid":["PMC4989828"],"doi":["10.4103/0971-5916.187103"],"title":["Zika virus: Indian perspectives."],"authorString":["Mourya DT, Shil P, Sapkal GN, Yadav PD."],"authorList":[{"author":[{"fullName":["Mourya DT"],"firstName":["Devendra T"],"lastName":["Mourya"],"initials":["DT"],"affiliation":["National Institute of Virology (ICMR), Pune, India."]},{"fullName":["Shil P"],"firstName":["Pratip"],"lastName":["Shil"],"initials":["P"],"affiliation":["National Institute of Virology (ICMR), Pune, India."]},{"fullName":["Sapkal GN"],"firstName":["Gajanan N"],"lastName":["Sapkal"],"initials":["GN"],"affiliation":["National Institute of Virology (ICMR), Pune, India."]},{"fullName":["Yadav PD"],"firstName":["Pragya D"],"lastName":["Yadav"],"initials":["PD"],"affiliation":["National Institute of Virology (ICMR), Pune, India."]}]}],"journalInfo":[{"issue":["5"],"volume":["143"],"journalIssueId":["2437265"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["The Indian journal of medical research"],"ISOAbbreviation":["Indian J. Med. Res."],"medlineAbbreviation":["Indian J Med Res"],"NLMid":["0374701"],"ISSN":["0971-5916"]}]}],"pubYear":["2016"],"pageInfo":["553-564"],"abstractText":["The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective."],"affiliation":["National Institute of Virology (ICMR), Pune, India."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4989828"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0971-5916.187103"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-09-02"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4989828"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDpJ9hGurOzZIcX","_score":1,"_source":{"id":["27593529"],"source":["MED"],"pmid":["27593529"],"pmcid":["PMC5011355"],"doi":["10.1186/s40249-016-0177-y"],"title":["Maintenance of demographic and hematological profiles in a long-lasting dengue fever outbreak: implications for management."],"authorString":["Dos Santos Carmo AM, Suzuki RB, Riquena MM, Eterovic A, Sperança MA."],"authorList":[{"author":[{"fullName":["Dos Santos Carmo AM"],"firstName":["Andréia Moreira"],"lastName":["Dos Santos Carmo"],"initials":["AM"],"affiliation":["Secretaria do Estado da Saúde do Estado de São Paulo, Instituto Adolfo Lutz, Centro de Laboratório Regional VIII, Santo André, 09040-160, São Paulo, Brazil."]},{"fullName":["Suzuki RB"],"firstName":["Rodrigo Buzinaro"],"lastName":["Suzuki"],"initials":["RB"],"affiliation":["Discipline of Parasitology, Marilia Medical School, Marilia, 17519-030, São Paulo, Brazil."]},{"fullName":["Riquena MM"],"firstName":["Michele Marcondes"],"lastName":["Riquena"],"initials":["MM"],"affiliation":["Center for Natural and Human Sciences, Universidade Federal do ABC, Campus São Bernardo do Campo, 09606-070, São Bernardo Do Campo, São Paulo, Brasil."]},{"fullName":["Eterovic A"],"firstName":["André"],"lastName":["Eterovic"],"initials":["A"],"affiliation":["Center for Natural and Human Sciences, Universidade Federal do ABC, Campus Santo André, Santo André, 09210-170, São Paulo, Brazil."]},{"fullName":["Sperança MA"],"firstName":["Márcia Aparecida"],"lastName":["Sperança"],"initials":["MA"],"affiliation":["Center for Natural and Human Sciences, Universidade Federal do ABC, Campus São Bernardo do Campo, 09606-070, São Bernardo Do Campo, São Paulo, Brasil. marcia.speranca@ufabc.edu.br."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2376921"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infectious diseases of poverty"],"ISOAbbreviation":["Infect Dis Poverty"],"medlineAbbreviation":["Infect Dis Poverty"],"NLMid":["101606645"],"ESSN":["2049-9957"]}]}],"pubYear":["2016"],"pageInfo":["84"],"abstractText":["Dengue fever (DF) outbreaks present regionally specific epidemiological and clinical characteristics. In certain medium-sized cities (100 000-250 000 inhabitants) of São Paulo State, Brazil, and after reaching an incidence of 150 cases/100 000 inhabitants (\"epidemiological threshold\"), clinical diagnosis indicated dengue virus (DENV) infection. During this period, other seasonally infectious diseases with symptoms and physical signs mimicking DF can simultaneously occur, with the consequential overcrowding of health care facilities as the principal drawbacks. Confirmation of clinical diagnosis of DF with serological tests may help in avoiding faulty diagnosis in patients, who might later undergo dengue hemorrhagic fever (DHF) and the dengue-shock syndrome (DSS). Furthermore, demographic and hematological profiles of patients are useful in detecting specific early characteristics associated to DF, DHF and DSS.From March to June, 2007, 456 patients from Marilia in northwest São Paulo State who had only been diagnosed for DF by clinical criteria, underwent serologic testing for non-structural 1 (NS1) DENV antigens. Individual results were used in comparative analysis according to demographic (gender, age) and hematological (leukocyte and platelet counts, percentage of atypical lymphocytes) profiles. Temporal patterns were evaluated by subdividing data according to time of initial attendance, using recorded variables as predictors of DENV infection in logistic regression models and ROC curves.Serologic DENV detection was positive in 70.6 % of the patients. Lower leukocyte and platelet counts were the most important factors in predicting DENV infection (respective medians DENV + = 3 715 cells/ml and DENV- = 6 760 cells/ml, and DENV + = 134 896 cells/ml and DENV- = 223 872 cells/ml). Furthermore, all demographic and hematological profiles presented a conservative temporal pattern throughout this long-lasting outbreak.As consistency throughout the epidemic facilitated defining the conservation pattern throughout the early stages, this was useful for improving management during the remaining period."],"affiliation":["Secretaria do Estado da Saúde do Estado de São Paulo, Instituto Adolfo Lutz, Centro de Laboratório Regional VIII, Santo André, 09040-160, São Paulo, Brazil."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Dengue fever","Clinical Diagnosis","Hematological Profile","Demographic Profile","Dengue- Outbreak Pattern","Epidemiological Threshold"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5011355?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5011355"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40249-016-0177-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-05"],"dateOfRevision":["2016-09-08"],"electronicPublicationDate":["2016-09-05"],"firstPublicationDate":["2016-09-05"],"cprojectID":"PMC5011355"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsHz_8VqgcuJwvkr","_score":1,"_source":{"id":["27367466"],"source":["MED"],"pmid":["27367466"],"pmcid":["PMC4994368"],"doi":["10.3201/eid2209.160664"],"title":["Inactivation and Environmental Stability of Zika Virus."],"authorString":["Müller JA, Harms M, Schubert A, Jansen S, Michel D, Mertens T, Schmidt-Chanasit J, Münch J."],"authorList":[{"author":[{"fullName":["Müller JA"],"firstName":["Janis A"],"lastName":["Müller"],"initials":["JA"]},{"fullName":["Harms M"],"firstName":["Mirja"],"lastName":["Harms"],"initials":["M"]},{"fullName":["Schubert A"],"firstName":["Axel"],"lastName":["Schubert"],"initials":["A"]},{"fullName":["Jansen S"],"firstName":["Stephanie"],"lastName":["Jansen"],"initials":["S"]},{"fullName":["Michel D"],"firstName":["Detlef"],"lastName":["Michel"],"initials":["D"]},{"fullName":["Mertens T"],"firstName":["Thomas"],"lastName":["Mertens"],"initials":["T"]},{"fullName":["Schmidt-Chanasit J"],"firstName":["Jonas"],"lastName":["Schmidt-Chanasit"],"initials":["J"]},{"fullName":["Münch J"],"firstName":["Jan"],"lastName":["Münch"],"initials":["J"]}]}],"journalInfo":[{"issue":["9"],"volume":["22"],"journalIssueId":["2409892"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1685-1687"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Inactivation","Vector-borne Infections","Flavivirus","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994368?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4994368"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2209.160664"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-17"],"dateOfRevision":["2016-09-08"],"electronicPublicationDate":["2016-09-15"],"firstPublicationDate":["2016-09-15"],"cprojectID":"PMC4994368"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsKC_8VqgcuJwvky","_score":1,"_source":{"id":["27057874"],"source":["MED"],"pmid":["27057874"],"pmcid":["PMC4998790"],"doi":["10.1097/md.0000000000003257"],"title":["Guillain-Barre Syndrome (42 Cases) Occurring During a Zika Virus Outbreak in French Polynesia."],"authorString":["Watrin L, Ghawché F, Larre P, Neau JP, Mathis S, Fournier E."],"authorList":[{"author":[{"fullName":["Watrin L"],"firstName":["Louise"],"lastName":["Watrin"],"initials":["L"],"affiliation":["From the Department of Neurology (LW, J-PN, SM), Poitiers University Hospital Center, Poitiers, France; Department of Neurology (FG, PL), French Polynesia Hospital Center, Papeete, Tahiti, French Polynesia; and Department of Clinical Neurophysiology (EF), La Pitié-Salpêtrière University Hospital Center, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Bd de l'Hôpital, Paris cedex, France."]},{"fullName":["Ghawché F"],"firstName":["Frédéric"],"lastName":["Ghawché"],"initials":["F"]},{"fullName":["Larre P"],"firstName":["Philippe"],"lastName":["Larre"],"initials":["P"]},{"fullName":["Neau JP"],"firstName":["Jean-Philippe"],"lastName":["Neau"],"initials":["JP"]},{"fullName":["Mathis S"],"firstName":["Stéphane"],"lastName":["Mathis"],"initials":["S"]},{"fullName":["Fournier E"],"firstName":["Emmanuel"],"lastName":["Fournier"],"initials":["E"]}]}],"journalInfo":[{"issue":["14"],"volume":["95"],"journalIssueId":["2395851"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Medicine"],"ISOAbbreviation":["Medicine (Baltimore)"],"medlineAbbreviation":["Medicine (Baltimore)"],"NLMid":["2985248R"],"ISSN":["0025-7974"],"ESSN":["1536-5964"]}]}],"pubYear":["2016"],"pageInfo":["e3257"],"abstractText":["Zika virus (transmitted by mosquitoes) reached French Polynesia for the first time in 2013, leading to an epidemic affecting 10% of the total population. So far, it has not been known to induce any neurological complications, but, a few weeks after the outbreak, an unexpectedly high number of 42 patients presented with Guillain-Barré syndrome.We report the clinical and electrophysiological characteristics of this series. Males predominated with a sex ratio of 2.82 (mean age: 46). All patients (except 2) were native Polynesian. At admission, 55% were able to walk unaided against 38% at nadir, 24% had swallowing troubles (nadir: 45%), 74% had motor weakness of the limbs (nadir: 86%) and deep tendon reflexes were diminished or not found in the vast majority of patients. Mean duration of the progressive phase and of the plateau phase was respectively 7 and 9 days. Thirty-eight percent of the patients were admitted in intensive care unit and 10 patients underwent tracheotomy. Nerve electrophysiological studies at admission showed marked distal motor conduction alterations, which had almost completely disappeared at the 4th month; this pattern was more suggestive of acute motor axonal neuropathy (AMAN) than of acute inflammatory demyelinating polyneuropathy (AIDP). Lumbar puncture showed elevated proteins in 90% of the cases, with cell count always inferior to 50/μL.This epidemic raises several questions, such as the potential existence of interactions between Zika virus and Polynesian HLA system and/or the consequences of several recombination events of this virus. This situation should call for increased vigilance, especially in countries where Aedes mosquitoes are present."],"affiliation":["From the Department of Neurology (LW, J-PN, SM), Poitiers University Hospital Center, Poitiers, France; Department of Neurology (FG, PL), French Polynesia Hospital Center, Papeete, Tahiti, French Polynesia; and Department of Clinical Neurophysiology (EF), La Pitié-Salpêtrière University Hospital Center, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Bd de l'Hôpital, Paris cedex, France."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Guillain-Barre Syndrome"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PP"],"qualifierName":["physiopathology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Retrospective Studies"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Polynesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Electrophysiological Phenomena"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4998790?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4998790"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1097/MD.0000000000003257"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-25"],"dateOfCreation":["2016-04-09"],"dateOfRevision":["2016-08-31"],"firstPublicationDate":["2016-04-01"],"cprojectID":"PMC4998790"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsMd_8VqgcuJwvk3","_score":1,"_source":{"id":["27575636"],"source":["MED"],"pmid":["27575636"],"pmcid":["PMC5004807"],"doi":["10.1371/journal.ppat.1005841"],"title":["The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing."],"authorString":["De Maio FA, Risso G, Iglesias NG, Shah P, Pozzi B, Gebhard LG, Mammi P, Mancini E, Yanovsky MJ, Andino R, Krogan N, Srebrow A, Gamarnik AV."],"authorList":[{"author":[{"fullName":["De Maio FA"],"firstName":["Federico A"],"lastName":["De Maio"],"initials":["FA"],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]},{"fullName":["Risso G"],"firstName":["Guillermo"],"lastName":["Risso"],"initials":["G"],"affiliation":["Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, UBA-CONICET), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires."]},{"fullName":["Iglesias NG"],"firstName":["Nestor G"],"lastName":["Iglesias"],"initials":["NG"],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]},{"fullName":["Shah P"],"firstName":["Priya"],"lastName":["Shah"],"initials":["P"],"affiliation":["Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California, United States of America."]},{"fullName":["Pozzi B"],"firstName":["Berta"],"lastName":["Pozzi"],"initials":["B"],"affiliation":["Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, UBA-CONICET), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires."]},{"fullName":["Gebhard LG"],"firstName":["Leopoldo G"],"lastName":["Gebhard"],"initials":["LG"],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]},{"fullName":["Mammi P"],"firstName":["Pablo"],"lastName":["Mammi"],"initials":["P"],"affiliation":["Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, UBA-CONICET), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires."]},{"fullName":["Mancini E"],"firstName":["Estefania"],"lastName":["Mancini"],"initials":["E"],"authorId":[{"_":"0000-0002-7308-1273","$":{"type":"ORCID"}}],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]},{"fullName":["Yanovsky MJ"],"firstName":["Marcelo J"],"lastName":["Yanovsky"],"initials":["MJ"],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]},{"fullName":["Andino R"],"firstName":["Raul"],"lastName":["Andino"],"initials":["R"],"affiliation":["Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, United States of America."]},{"fullName":["Krogan N"],"firstName":["Nevan"],"lastName":["Krogan"],"initials":["N"],"affiliation":["Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California, United States of America."]},{"fullName":["Srebrow A"],"firstName":["Anabella"],"lastName":["Srebrow"],"initials":["A"],"affiliation":["Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, UBA-CONICET), Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires."]},{"fullName":["Gamarnik AV"],"firstName":["Andrea V"],"lastName":["Gamarnik"],"initials":["AV"],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7308-1273","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["12"],"journalIssueId":["2436285"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005841"],"abstractText":["Dengue virus NS5 protein plays multiple functions in the cytoplasm of infected cells, enabling viral RNA replication and counteracting host antiviral responses. Here, we demonstrate a novel function of NS5 in the nucleus where it interferes with cellular splicing. Using global proteomic analysis of infected cells together with functional studies, we found that NS5 binds spliceosome complexes and modulates endogenous splicing as well as minigene-derived alternative splicing patterns. In particular, we show that NS5 alone, or in the context of viral infection, interacts with core components of the U5 snRNP particle, CD2BP2 and DDX23, alters the inclusion/exclusion ratio of alternative splicing events, and changes mRNA isoform abundance of known antiviral factors. Interestingly, a genome wide transcriptome analysis, using recently developed bioinformatics tools, revealed an increase of intron retention upon dengue virus infection, and viral replication was improved by silencing specific U5 components. Different mechanistic studies indicate that binding of NS5 to the spliceosome reduces the efficiency of pre-mRNA processing, independently of NS5 enzymatic activities. We propose that NS5 binding to U5 snRNP proteins hijacks the splicing machinery resulting in a less restrictive environment for viral replication."],"affiliation":["Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5004807?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5004807"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005841"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-31"],"electronicPublicationDate":["2016-08-30"],"firstPublicationDate":["2016-08-30"],"cprojectID":"PMC5004807"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8__8VqgcuJwvkJ","_score":1,"_source":{"id":["27333025"],"source":["MED"],"pmid":["27333025"],"pmcid":["PMC4972568"],"doi":["10.1016/j.ebiom.2016.06.020"],"title":["The When and the Where of Zika Epidemic Potential in Europe - An Evidence Base for Public Health Preparedness."],"authorString":["Paul RE."],"authorList":[{"author":[{"fullName":["Paul RE"],"firstName":["Richard E"],"lastName":["Paul"],"initials":["RE"],"affiliation":["Functional Genetics of Infectious Diseases Unit, Institut Pasteur, Paris, France."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2435137"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["17-18"],"affiliation":["Functional Genetics of Infectious Diseases Unit, Institut Pasteur, Paris, France."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","Mosquitoes","Flavivirus","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972568?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4972568"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.06.020"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-01"],"dateOfRevision":["2016-08-12"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4972568"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRM_8VqgcuJwvh0","_score":1,"_source":{"id":["27134823"],"source":["MED"],"pmid":["27134823"],"pmcid":["PMC4833829"],"doi":["10.1016/j.idcr.2016.03.004"],"title":["Zika virus infections imported from Brazil to Portugal, 2015."],"authorString":["Zé-Zé L, Prata MB, Teixeira T, Marques N, Mondragão A, Fernandes R, Saraiva da Cunha J, Alves MJ."],"authorList":[{"author":[{"fullName":["Zé-Zé L"],"firstName":["L"],"lastName":["Zé-Zé"],"initials":["L"],"authorId":[{"_":"0000-0001-7258-1439","$":{"type":"ORCID"}}],"affiliation":["Centro de Estudos de Vectores e Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Águas de Moura, Portugal; Biosystems & Integrative Sciences Institute, University of Lisbon, Faculty of Sciences, Campo Grande, Lisbon, Portugal."]},{"fullName":["Prata MB"],"firstName":["M B"],"lastName":["Prata"],"initials":["MB"],"affiliation":["Serviço de Doenças Infecciosas, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal."]},{"fullName":["Teixeira T"],"firstName":["T"],"lastName":["Teixeira"],"initials":["T"],"affiliation":["Unidade de Doenças Infecciosas/Serviço de Medicina Interna, Centro Hospitalar de Vila Nova de Gaia/Espinho, Vila Nova de Gaia, Portugal."]},{"fullName":["Marques N"],"firstName":["N"],"lastName":["Marques"],"initials":["N"],"affiliation":["Serviço de Doenças Infecciosas, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal."]},{"fullName":["Mondragão A"],"firstName":["A"],"lastName":["Mondragão"],"initials":["A"],"affiliation":["Unidade de Doenças Infecciosas/Serviço de Medicina Interna, Centro Hospitalar de Vila Nova de Gaia/Espinho, Vila Nova de Gaia, Portugal."]},{"fullName":["Fernandes R"],"firstName":["R"],"lastName":["Fernandes"],"initials":["R"],"affiliation":["Unidade de Doenças Infecciosas/Serviço de Medicina Interna, Centro Hospitalar de Vila Nova de Gaia/Espinho, Vila Nova de Gaia, Portugal."]},{"fullName":["Saraiva da Cunha J"],"firstName":["J"],"lastName":["Saraiva da Cunha"],"initials":["J"],"affiliation":["Serviço de Doenças Infecciosas, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal."]},{"fullName":["Alves MJ"],"firstName":["M J"],"lastName":["Alves"],"initials":["MJ"],"affiliation":["Centro de Estudos de Vectores e Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Águas de Moura, Portugal."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7258-1439","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2395180"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["IDCases"],"ISOAbbreviation":["IDCases"],"medlineAbbreviation":["IDCases"],"NLMid":["101634540"],"ESSN":["2214-2509"]}]}],"pubYear":["2016"],"pageInfo":["46-49"],"abstractText":["Zika virus is an emerging arbovirus transmitted by Aedes sp. mosquitoes like the Dengue and Chikungunya viruses. Zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. In 2007, an epidemic in Micronesia initiated the turnover in the epidemiological history of Zika virus and more recently, the potential association with congenital microcephaly cases in Brazil 2015, still under investigation, led the World Health Organization (WHO) to declare a Public Health Emergency of International Concern on February 1, 2016. Here, we present the clinical and laboratory aspects related to the first four imported human cases of Zika virus in Portugal from Brazil, and alert, regarding the high level of traveling between Portugal and Brazil, and the ongoing expansion of this virus in the Americas, for the threat for Zika virus introduction in Europe and the possible introduction to Madeira Island where Aedes aegypti is present."],"affiliation":["Centro de Estudos de Vectores e Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Águas de Moura, Portugal; Biosystems & Integrative Sciences Institute, University of Lisbon, Faculty of Sciences, Campo Grande, Lisbon, Portugal."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Arbovirus","Imported Cases","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4833829?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4833829"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.idcr.2016.03.004"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-02"],"dateOfCreation":["2016-05-02"],"dateOfRevision":["2016-05-04"],"electronicPublicationDate":["2016-04-07"],"firstPublicationDate":["2016-04-07"],"cprojectID":"PMC4833829"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrWy_8VqgcuJwviC","_score":1,"_source":{"id":["26962084"],"source":["MED"],"pmid":["26962084"],"pmcid":["PMC4844727"],"doi":["10.1128/jcm.00113-16"],"title":["Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus."],"authorString":["Lagerqvist N, Hagström Å, Lundahl M, Nilsson E, Juremalm M, Larsson I, Alm E, Bucht G, Ahlm C, Klingström J."],"authorList":[{"author":[{"fullName":["Lagerqvist N"],"firstName":["Nina"],"lastName":["Lagerqvist"],"initials":["N"],"affiliation":["Public Health Agency of Sweden, Solna, Sweden Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden."]},{"fullName":["Hagström Å"],"firstName":["Åsa"],"lastName":["Hagström"],"initials":["Å"],"affiliation":["National Veterinary Institute, Uppsala, Sweden."]},{"fullName":["Lundahl M"],"firstName":["Malin"],"lastName":["Lundahl"],"initials":["M"],"affiliation":["Public Health Agency of Sweden, Solna, Sweden."]},{"fullName":["Nilsson E"],"firstName":["Elin"],"lastName":["Nilsson"],"initials":["E"],"affiliation":["Swedish Defence Research Agency, Umeå, Sweden."]},{"fullName":["Juremalm M"],"firstName":["Mikael"],"lastName":["Juremalm"],"initials":["M"],"affiliation":["National Veterinary Institute, Uppsala, Sweden."]},{"fullName":["Larsson I"],"firstName":["Inger"],"lastName":["Larsson"],"initials":["I"],"affiliation":["Sunderbyn Hospital, Luleå, Sweden."]},{"fullName":["Alm E"],"firstName":["Erik"],"lastName":["Alm"],"initials":["E"],"affiliation":["Public Health Agency of Sweden, Solna, Sweden."]},{"fullName":["Bucht G"],"firstName":["Göran"],"lastName":["Bucht"],"initials":["G"],"affiliation":["Swedish Defence Research Agency, Umeå, Sweden."]},{"fullName":["Ahlm C"],"firstName":["Clas"],"lastName":["Ahlm"],"initials":["C"],"affiliation":["Department of Clinical Microbiology, Infectious Diseases, Umeå University, Umeå, Sweden."]},{"fullName":["Klingström J"],"firstName":["Jonas"],"lastName":["Klingström"],"initials":["J"],"affiliation":["Public Health Agency of Sweden, Solna, Sweden Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden jonas.klingstrom@ki.se."]}]}],"journalInfo":[{"issue":["5"],"volume":["54"],"journalIssueId":["2401473"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Journal of clinical microbiology"],"ISOAbbreviation":["J. Clin. Microbiol."],"medlineAbbreviation":["J Clin Microbiol"],"NLMid":["7505564"],"ISSN":["0095-1137"],"ESSN":["1098-660X"]}]}],"pubYear":["2016"],"pageInfo":["1335-1339"],"abstractText":["Rodent-borne hantaviruses cause two severe acute diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus pulmonary syndrome (HPS; also called hantavirus cardiopulmonary syndrome [HCPS]) in the Americas. Puumala virus (PUUV) is the most common causative agent of HFRS in Europe. Current routine diagnostic methods are based on serological analyses and can yield inconclusive results. Hantavirus-infected patients are viremic during the early phase of disease; therefore, detection of viral RNA genomes can be a valuable complement to existing serological methods. However, the high genomic sequence diversity of PUUV has hampered the development of molecular diagnostics, and currently no real-time reverse transcription-quantitative (RT)-PCR assay is available for routine diagnosis of HFRS. Here, we present a novel PUUV RT-PCR assay. The assay was validated for routine diagnosis of HFRS on samples collected in Sweden during the winter season from 2013 to 2014. The assay allowed detection of PUUV RNA in 98.7% of confirmed clinical HFRS samples collected within 8 days after symptomatic onset. In summary, this study shows that real-time RT-PCR can be a reliable alternative to serological tests during the early phase of HFRS."],"affiliation":["Public Health Agency of Sweden, Solna, Sweden Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4844727?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4844727"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/JCM.00113-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-04-26"],"dateOfRevision":["2016-05-07"],"electronicPublicationDate":["2016-03-09"],"firstPublicationDate":["2016-03-09"],"cprojectID":"PMC4844727"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYTJ9hGurOzZIaG","_score":1,"_source":{"id":["27195954"],"source":["MED"],"pmid":["27195954"],"pmcid":["PMC4873000"],"doi":["10.1371/journal.pmed.1002042"],"title":["Toward a Common Secure Future: Four Global Commissions in the Wake of Ebola."],"authorString":["Gostin LO, Tomori O, Wibulpolprasert S, Jha AK, Frenk J, Moon S, Phumaphi J, Piot P, Stocking B, Dzau VJ, Leung GM."],"authorList":[{"author":[{"fullName":["Gostin LO"],"firstName":["Lawrence O"],"lastName":["Gostin"],"initials":["LO"],"affiliation":["O'Neill Institute for National and Global Health Law, Georgetown University, Washington, DC, United States of America."]},{"fullName":["Tomori O"],"firstName":["Oyewale"],"lastName":["Tomori"],"initials":["O"],"affiliation":["Nigeria Academy of Sciences, Lagos, Nigeria."]},{"fullName":["Wibulpolprasert S"],"firstName":["Suwit"],"lastName":["Wibulpolprasert"],"initials":["S"],"affiliation":["Ministry of Public Health, Nonthaburi, Thailand."]},{"fullName":["Jha AK"],"firstName":["Ashish K"],"lastName":["Jha"],"initials":["AK"],"affiliation":["Harvard T.H. Chan School of Public Health, Boston, Massachusetts, United States of America."]},{"fullName":["Frenk J"],"firstName":["Julio"],"lastName":["Frenk"],"initials":["J"],"authorId":[{"_":"0000-0002-8760-7926","$":{"type":"ORCID"}}],"affiliation":["University of Miami, Coral Gables, Florida, United States of America."]},{"fullName":["Moon S"],"firstName":["Suerie"],"lastName":["Moon"],"initials":["S"],"affiliation":["Harvard Global Health Institute, Harvard University, Cambridge, Massachusetts, United States of America."]},{"fullName":["Phumaphi J"],"firstName":["Joy"],"lastName":["Phumaphi"],"initials":["J"],"affiliation":["African Leaders Malaria Alliance, New York, New York, United States of America."]},{"fullName":["Piot P"],"firstName":["Peter"],"lastName":["Piot"],"initials":["P"],"affiliation":["London School of Hygiene & Tropical Medicine, London, United Kingdom."]},{"fullName":["Stocking B"],"firstName":["Barbara"],"lastName":["Stocking"],"initials":["B"],"affiliation":["Murray Edwards College, University of Cambridge, United Kingdom."]},{"fullName":["Dzau VJ"],"firstName":["Victor J"],"lastName":["Dzau"],"initials":["VJ"],"affiliation":["National Academy of Medicine, Washington, DC, United States of America."]},{"fullName":["Leung GM"],"firstName":["Gabriel M"],"lastName":["Leung"],"initials":["GM"],"affiliation":["Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8760-7926","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["13"],"journalIssueId":["2403902"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS medicine"],"ISOAbbreviation":["PLoS Med."],"medlineAbbreviation":["PLoS Med"],"NLMid":["101231360"],"ISSN":["1549-1277"],"ESSN":["1549-1676"]}]}],"pubYear":["2016"],"pageInfo":["e1002042"],"abstractText":["Lawrence Gostin and colleagues offer a set of priorities for global health preparedness and response for future infectious disease threats."],"affiliation":["O'Neill Institute for National and Global Health Law, Georgetown University, Washington, DC, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4873000?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4873000"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pmed.1002042"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-20"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-19"],"firstPublicationDate":["2016-05-19"],"cprojectID":"PMC4873000"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYV_8VqgcuJwviL","_score":1,"_source":{"id":["27149521"],"source":["MED"],"pmid":["27149521"],"pmcid":["PMC4858159"],"doi":["10.1371/journal.pntd.0004658"],"title":["A Susceptible Mouse Model for Zika Virus Infection."],"authorString":["Dowall SD, Graham VA, Rayner E, Atkinson B, Hall G, Watson RJ, Bosworth A, Bonney LC, Kitchen S, Hewson R."],"authorList":[{"author":[{"fullName":["Dowall SD"],"firstName":["Stuart D"],"lastName":["Dowall"],"initials":["SD"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Graham VA"],"firstName":["Victoria A"],"lastName":["Graham"],"initials":["VA"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Rayner E"],"firstName":["Emma"],"lastName":["Rayner"],"initials":["E"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Atkinson B"],"firstName":["Barry"],"lastName":["Atkinson"],"initials":["B"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Hall G"],"firstName":["Graham"],"lastName":["Hall"],"initials":["G"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Watson RJ"],"firstName":["Robert J"],"lastName":["Watson"],"initials":["RJ"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Bosworth A"],"firstName":["Andrew"],"lastName":["Bosworth"],"initials":["A"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Bonney LC"],"firstName":["Laura C"],"lastName":["Bonney"],"initials":["LC"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Kitchen S"],"firstName":["Samantha"],"lastName":["Kitchen"],"initials":["S"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]},{"fullName":["Hewson R"],"firstName":["Roger"],"lastName":["Hewson"],"initials":["R"],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004658"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne pathogen which has recently spread beyond Africa and into Pacific and South American regions. Despite first being detected in 1947, very little information is known about the virus, and its spread has been associated with increases in Guillain-Barre syndrome and microcephaly. There are currently no known vaccines or antivirals against ZIKV infection. Progress in assessing interventions will require the development of animal models to test efficacies; however, there are only limited reports on in vivo studies. The only susceptible murine models have involved intracerebral inoculations or juvenile animals, which do not replicate natural infection. Our report has studied the effect of ZIKV infection in type-I interferon receptor deficient (A129) mice and the parent strain (129Sv/Ev) after subcutaneous challenge in the lower leg to mimic a mosquito bite. A129 mice developed severe symptoms with widespread viral RNA detection in the blood, brain, spleen, liver and ovaries. Histological changes were also striking in these animals. 129Sv/Ev mice developed no clinical symptoms or histological changes, despite viral RNA being detectable in the blood, spleen and ovaries, albeit at lower levels than those seen in A129 mice. Our results identify A129 mice as being highly susceptible to ZIKV and thus A129 mice represent a suitable, and urgently required, small animal model for the testing of vaccines and antivirals."],"affiliation":["National Infection Service, Public Health England, Porton Down, Salisbury, Wiltshire, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858159?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858159"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004658"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-05-06"],"dateOfRevision":["2016-05-14"],"electronicPublicationDate":["2016-05-05"],"firstPublicationDate":["2016-05-05"],"cprojectID":"PMC4858159"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrZD_8VqgcuJwviO","_score":1,"_source":{"id":["27088970"],"source":["MED"],"pmid":["27088970"],"pmcid":["PMC4861529"],"doi":["10.3201/eid2205.151915"],"title":["Isolation of Zika Virus from Febrile Patient, Indonesia."],"authorString":["Perkasa A, Yudhaputri F, Haryanto S, Hayati RF, Ma'roef CN, Antonjaya U, Yohan B, Myint KS, Ledermann JP, Rosenberg R, Powers AM, Sasmono RT."],"authorList":[{"author":[{"fullName":["Perkasa A"],"firstName":["Aditya"],"lastName":["Perkasa"],"initials":["A"]},{"fullName":["Yudhaputri F"],"firstName":["Frilasita"],"lastName":["Yudhaputri"],"initials":["F"]},{"fullName":["Haryanto S"],"firstName":["Sotianingsih"],"lastName":["Haryanto"],"initials":["S"]},{"fullName":["Hayati RF"],"firstName":["Rahma F"],"lastName":["Hayati"],"initials":["RF"]},{"fullName":["Ma'roef CN"],"firstName":["Chairin Nisa"],"lastName":["Ma'roef"],"initials":["CN"]},{"fullName":["Antonjaya U"],"firstName":["Ungke"],"lastName":["Antonjaya"],"initials":["U"]},{"fullName":["Yohan B"],"firstName":["Benediktus"],"lastName":["Yohan"],"initials":["B"],"authorId":[{"_":"0000-0003-2378-2386","$":{"type":"ORCID"}}]},{"fullName":["Myint KS"],"firstName":["Khin Saw Aye"],"lastName":["Myint"],"initials":["KS"]},{"fullName":["Ledermann JP"],"firstName":["Jeremy P"],"lastName":["Ledermann"],"initials":["JP"]},{"fullName":["Rosenberg R"],"firstName":["Ronald"],"lastName":["Rosenberg"],"initials":["R"]},{"fullName":["Powers AM"],"firstName":["Ann M"],"lastName":["Powers"],"initials":["AM"]},{"fullName":["Sasmono RT"],"firstName":["R Tedjo"],"lastName":["Sasmono"],"initials":["RT"],"authorId":[{"_":"0000-0003-0986-2590","$":{"type":"ORCID"}}]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2378-2386","$":{"type":"ORCID"}},{"_":"0000-0003-0986-2590","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["924-925"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S.","Research Support, U.S. Gov't, P.H.S."]}],"keywordList":[{"keyword":["Viruses","Indonesia","Arbovirus","Vector-borne Infections","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861529?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861529"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.151915"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL"]}],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861529"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrZShOUS2orL1IQZ","_score":1,"_source":{"id":["27205899"],"source":["MED"],"pmid":["27205899"],"pmcid":["PMC4874682"],"doi":["10.1371/journal.pntd.0004743"],"title":["A Cost-Effectiveness Tool for Informing Policies on Zika Virus Control."],"authorString":["Alfaro-Murillo JA, Parpia AS, Fitzpatrick MC, Tamagnan JA, Medlock J, Ndeffo-Mbah ML, Fish D, Ávila-Agüero ML, Marín R, Ko AI, Galvani AP."],"authorList":[{"author":[{"fullName":["Alfaro-Murillo JA"],"firstName":["Jorge A"],"lastName":["Alfaro-Murillo"],"initials":["JA"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Parpia AS"],"firstName":["Alyssa S"],"lastName":["Parpia"],"initials":["AS"],"authorId":[{"_":"0000-0002-9083-1929","$":{"type":"ORCID"}}],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Fitzpatrick MC"],"firstName":["Meagan C"],"lastName":["Fitzpatrick"],"initials":["MC"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Tamagnan JA"],"firstName":["Jules A"],"lastName":["Tamagnan"],"initials":["JA"],"affiliation":["Center for Infectious Disease Modeling and Analysis, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Medlock J"],"firstName":["Jan"],"lastName":["Medlock"],"initials":["J"],"affiliation":["Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, United States of America."]},{"fullName":["Ndeffo-Mbah ML"],"firstName":["Martial L"],"lastName":["Ndeffo-Mbah"],"initials":["ML"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Fish D"],"firstName":["Durland"],"lastName":["Fish"],"initials":["D"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."]},{"fullName":["Ávila-Agüero ML"],"firstName":["María L"],"lastName":["Ávila-Agüero"],"initials":["ML"],"affiliation":["Pediatric Infectious Diseases Department, Hospital Nacional de Niños \"Dr. Carlos Sáenz Herrera\", San José, Costa Rica."]},{"fullName":["Marín R"],"firstName":["Rodrigo"],"lastName":["Marín"],"initials":["R"],"affiliation":["Programa de Control de Vectores, Ministerio de Salud, San José, Costa Rica."]},{"fullName":["Ko AI"],"firstName":["Albert I"],"lastName":["Ko"],"initials":["AI"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Saúde, Salvador, Bahia, Brasil."]},{"fullName":["Galvani AP"],"firstName":["Alison P"],"lastName":["Galvani"],"initials":["AP"],"affiliation":["Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9083-1929","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004743"],"abstractText":["As Zika virus continues to spread, decisions regarding resource allocations to control the outbreak underscore the need for a tool to weigh policies according to their cost and the health burden they could avert. For example, to combat the current Zika outbreak the US President requested the allocation of $1.8 billion from Congress in February 2016.Illustrated through an interactive tool, we evaluated how the number of Zika cases averted, the period during pregnancy in which Zika infection poses a risk of microcephaly, and probabilities of microcephaly and Guillain-Barré Syndrome (GBS) impact the cost at which an intervention is cost-effective. From Northeast Brazilian microcephaly incidence data, we estimated the probability of microcephaly in infants born to Zika-infected women (0.49% to 2.10%). We also estimated the probability of GBS arising from Zika infections in Brazil (0.02% to 0.06%) and Colombia (0.08%). We calculated that each microcephaly and GBS case incurs the loss of 29.95 DALYs and 1.25 DALYs per case, as well as direct medical costs for Latin America and the Caribbean of $91,102 and $28,818, respectively. We demonstrated the utility of our cost-effectiveness tool with examples evaluating funding commitments by Costa Rica and Brazil, the US presidential proposal, and the novel approach of genetically modified mosquitoes. Our analyses indicate that the commitments and the proposal are likely to be cost-effective, whereas the cost-effectiveness of genetically modified mosquitoes depends on the country of implementation.Current estimates from our tool suggest that the health burden from microcephaly and GBS warrants substantial expenditures focused on Zika virus control. Our results justify the funding committed in Costa Rica and Brazil and many aspects of the budget outlined in the US president's proposal. As data continue to be collected, new parameter estimates can be customized in real-time within our user-friendly tool to provide updated estimates on cost-effectiveness of interventions and inform policy decisions in country-specific settings."],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4874682?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4874682"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004743"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-21"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-20"],"firstPublicationDate":["2016-05-20"],"cprojectID":"PMC4874682"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraLhOUS2orL1IQc","_score":1,"_source":{"id":["27143490"],"source":["MED"],"pmid":["27143490"],"pmcid":["PMC4878297"],"doi":["10.1590/0074-02760160085"],"title":["Zika virus damages the human placental barrier and presents marked fetal neurotropism."],"authorString":["Noronha Ld, Zanluca C, Azevedo ML, Luz KG, Santos CN."],"authorList":[{"author":[{"fullName":["Noronha Ld"],"firstName":["Lucia de"],"lastName":["Noronha"],"initials":["Ld"],"affiliation":["Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil."]},{"fullName":["Zanluca C"],"firstName":["Camila"],"lastName":["Zanluca"],"initials":["C"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."]},{"fullName":["Azevedo ML"],"firstName":["Marina Luize Viola"],"lastName":["Azevedo"],"initials":["ML"],"affiliation":["Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil."]},{"fullName":["Luz KG"],"firstName":["Kleber Giovanni"],"lastName":["Luz"],"initials":["KG"],"affiliation":["Instituto de Medicina Tropical, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil."]},{"fullName":["Santos CN"],"firstName":["Claudia Nunes Duarte Dos"],"lastName":["Santos"],"initials":["CN"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."]}]}],"journalInfo":[{"issue":["5"],"volume":["111"],"journalIssueId":["2412036"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["287-293"],"abstractText":["An unusually high incidence of microcephaly in newborns has recently been observed in Brazil. There is a temporal association between the increase in cases of microcephaly and the Zika virus (ZIKV) epidemic. Viral RNA has been detected in amniotic fluid samples, placental tissues and newborn and fetal brain tissues. However, much remains to be determined concerning the association between ZIKV infection and fetal malformations. In this study, we provide evidence of the transplacental transmission of ZIKV through the detection of viral proteins and viral RNA in placental tissue samples from expectant mothers infected at different stages of gestation. We observed chronic placentitis (TORCH type) with viral protein detection by immunohistochemistry in Hofbauer cells and some histiocytes in the intervillous spaces. We also demonstrated the neurotropism of the virus via the detection of viral proteins in glial cells and in some endothelial cells and the observation of scattered foci of microcalcifications in the brain tissues. Lesions were mainly located in the white matter. ZIKV RNA was also detected in these tissues by real-time-polymerase chain reaction. We believe that these findings will contribute to the body of knowledge of the mechanisms of ZIKV transmission, interactions between the virus and host cells and viral tropism."],"affiliation":["Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878297?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878297"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160085"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-26"],"dateOfRevision":["2016-05-26"],"electronicPublicationDate":["2016-04-29"],"firstPublicationDate":["2016-04-29"],"cprojectID":"PMC4878297"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrbdhOUS2orL1IQl","_score":1,"_source":{"id":["27164125"],"source":["MED"],"pmid":["27164125"],"pmcid":["PMC4885077"],"doi":["10.3390/v8050122"],"title":["Dengue Virus Reporter Replicon is a Valuable Tool for Antiviral Drug Discovery and Analysis of Virus Replication Mechanisms."],"authorString":["Kato F, Hishiki T."],"authorList":[{"author":[{"fullName":["Kato F"],"firstName":["Fumihiro"],"lastName":["Kato"],"initials":["F"],"affiliation":["Department of Virology 1, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. fumihiro@nih.go.jp."]},{"fullName":["Hishiki T"],"firstName":["Takayuki"],"lastName":["Hishiki"],"initials":["T"],"affiliation":["Viral Infectious Diseases Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan. hishiki-tk@igakuken.or.jp."]}]}],"journalInfo":[{"issue":["5"],"volume":["8"],"journalIssueId":["2403542"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2016"],"abstractText":["Dengue, the most prevalent arthropod-borne viral disease, is caused by the dengue virus (DENV), a member of the Flaviviridae family, and is a considerable public health threat in over 100 countries, with 2.5 billion people living in high-risk areas. However, no specific antiviral drug or licensed vaccine currently targets DENV infection. The replicon system has all the factors needed for viral replication in cells. Since the development of replicon systems, transient and stable reporter replicons, as well as reporter viruses, have been used in the study of various virological aspects of DENV and in the identification of DENV inhibitors. In this review, we summarize the DENV reporter replicon system and its applications in high-throughput screening (HTS) for identification of anti-DENV inhibitors. We also describe the use of this system in elucidation of the mechanisms of virus replication and viral dynamics in vivo and in vitro."],"affiliation":["Department of Virology 1, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. fumihiro@nih.go.jp."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Dengue virus","Replication","High-throughput screening","Flavivirus","Antiviral Drug","Reporter Replicon"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4885077?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4885077"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v8050122"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-11"],"dateOfRevision":["2016-06-01"],"electronicPublicationDate":["2016-05-05"],"firstPublicationDate":["2016-05-05"],"cprojectID":"PMC4885077"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrb7_8VqgcuJwviY","_score":1,"_source":{"id":["27171170"],"source":["MED"],"pmid":["27171170"],"pmcid":["PMC4865232"],"doi":["10.1371/journal.ppat.1005548"],"title":["Determinants of Arbovirus Vertical Transmission in Mosquitoes."],"authorString":["Lequime S, Paul RE, Lambrechts L."],"authorList":[{"author":[{"fullName":["Lequime S"],"firstName":["Sebastian"],"lastName":["Lequime"],"initials":["S"],"authorId":[{"_":"0000-0002-3140-0651","$":{"type":"ORCID"}}],"affiliation":["Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France."]},{"fullName":["Paul RE"],"firstName":["Richard E"],"lastName":["Paul"],"initials":["RE"],"affiliation":["Functional Genetics of Infectious Diseases Unit, Department of Genomes and Genetics, Institut Pasteur, Paris, France."]},{"fullName":["Lambrechts L"],"firstName":["Louis"],"lastName":["Lambrechts"],"initials":["L"],"authorId":[{"_":"0000-0001-5958-2138","$":{"type":"ORCID"}}],"affiliation":["Centre National de la Recherche Scientifique, Unité de Recherche Associée 3012, Paris, France."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3140-0651","$":{"type":"ORCID"}},{"_":"0000-0001-5958-2138","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["12"],"journalIssueId":["2403739"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005548"],"abstractText":["Vertical transmission (VT) and horizontal transmission (HT) of pathogens refer to parental and non-parental chains of host-to-host transmission. Combining HT with VT enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. Elucidating these factors is particularly important for understanding the epidemiology of arthropod-borne viruses (arboviruses) of public health significance. Arboviruses are primarily maintained by HT between arthropod vectors and vertebrate hosts in nature, but are occasionally transmitted vertically in the vector population from an infected female to her offspring, which is a proposed maintenance mechanism during adverse conditions for HT. Here, we review over a century of published primary literature on natural and experimental VT, which we previously assembled into large databases, to identify biological factors associated with the efficiency of arbovirus VT in mosquito vectors. Using a robust statistical framework, we highlight a suite of environmental, taxonomic, and physiological predictors of arbovirus VT. These novel insights contribute to refine our understanding of strategies employed by arboviruses to persist in the environment and cause substantial public health concern. They also provide hypotheses on the biological processes underlying the relative VT frequency for pathogens with mixed-mode transmission that can be tested empirically."],"affiliation":["Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4865232?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4865232"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005548"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-13"],"dateOfRevision":["2016-05-27"],"electronicPublicationDate":["2016-05-12"],"firstPublicationDate":["2016-05-12"],"cprojectID":"PMC4865232"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrd9_8VqgcuJwvif","_score":1,"_source":{"id":["27187593"],"source":["MED"],"pmid":["27187593"],"pmcid":["PMC4871372"],"doi":["10.1371/journal.pone.0152805"],"title":["Personal Protection of Permethrin-Treated Clothing against Aedes aegypti, the Vector of Dengue and Zika Virus, in the Laboratory."],"authorString":["Orsborne J, DeRaedt Banks S, Hendy A, Gezan SA, Kaur H, Wilder-Smith A, Lindsay SW, Logan JG."],"authorList":[{"author":[{"fullName":["Orsborne J"],"firstName":["James"],"lastName":["Orsborne"],"initials":["J"],"affiliation":["arctec, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["DeRaedt Banks S"],"firstName":["Sarah"],"lastName":["DeRaedt Banks"],"initials":["S"],"affiliation":["Department of Disease Control, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Hendy A"],"firstName":["Adam"],"lastName":["Hendy"],"initials":["A"],"affiliation":["Department of Disease Control, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Gezan SA"],"firstName":["Salvador A"],"lastName":["Gezan"],"initials":["SA"],"affiliation":["SFRC, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Kaur H"],"firstName":["Harparkash"],"lastName":["Kaur"],"initials":["H"],"affiliation":["Department of Disease Control, London School of Hygiene and Tropical Medicine, London, United Kingdom."]},{"fullName":["Wilder-Smith A"],"firstName":["Annelies"],"lastName":["Wilder-Smith"],"initials":["A"],"affiliation":["Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore."]},{"fullName":["Lindsay SW"],"firstName":["Steve W"],"lastName":["Lindsay"],"initials":["SW"],"affiliation":["School of Biological and Biomedical Sciences, Durham University, Durham, United Kingdom."]},{"fullName":["Logan JG"],"firstName":["James G"],"lastName":["Logan"],"initials":["JG"],"affiliation":["arctec, London School of Hygiene and Tropical Medicine, London, United Kingdom."]}]}],"journalInfo":[{"issue":["5"],"volume":["11"],"journalIssueId":["2403348"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0152805"],"abstractText":["The dengue and Zika viruses are primarily transmitted by Aedes aegypti mosquitoes, which are most active during day light hours and feed both in and outside of the household. Personal protection technologies such as insecticide-treated clothing could provide individual protection. Here we assessed the efficacy of permethrin-treated clothing on personal protection in the laboratory.The effect of washing on treated clothing, skin coverage and protection against resistant and susceptible Ae. aegypti was assessed using modified WHO arm-in-cage assays. Coverage was further assessed using free-flight room tests to investigate the protective efficacy of unwashed factory-dipped permethrin-treated clothing. Clothing was worn as full coverage (long sleeves and trousers) and partial coverage (short sleeves and shorts). Residual permethrin on the skin and its effect on mosquitoes was measured using modified WHO cone assays and quantified using high-pressure liquid chromatography (HPLC) analysis.In the arm-in-cage assays, unwashed clothing reduced landing by 58.9% (95% CI 49.2-66.9) and biting by 28.5% (95% CI 22.5-34.0), but reduced to 18.5% (95% CI 14.7-22.3) and 11.1% (95% CI 8.5-13.8) respectively after 10 washes. Landing and biting for resistant and susceptible strains was not significantly different (p<0.05). In free-flight room tests, full coverage treated clothing reduced landing by 24.3% (95% CI 17.4-31.7) and biting by 91% (95% CI 82.2-95.9) with partial coverage reducing landing and biting by 26.4% (95% CI 20.3-31.2) and 49.3% (95% CI 42.1-59.1) respectively with coverage type having no significant difference on landing (p<0.05). Residual permethrin was present on the skin in low amounts (0.0041mg/cm2), but still produced a KD of >80% one hour after wearing treated clothing.Whilst partially covering the body with permethrin-treated clothing provided some protection against biting, wearing treated clothing with long sleeves and trousers provided the highest form of protection. Washing treated clothing dramatically reduced protection provided. Permethrin-treated clothing could provide protection to individuals from Ae. aegypti that show permethrin resistance. Additionally, it could continue to provide protection even after the clothing has been worn. Field trials are urgently needed to determine whether clothing can protect against dengue and Zika."],"affiliation":["arctec, London School of Hygiene and Tropical Medicine, London, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871372?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871372"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0152805"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-18"],"dateOfRevision":["2016-06-01"],"electronicPublicationDate":["2016-05-17"],"firstPublicationDate":["2016-05-17"],"cprojectID":"PMC4871372"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrd-_8VqgcuJwvig","_score":1,"_source":{"id":["27203689"],"source":["MED"],"pmid":["27203689"],"pmcid":["PMC4874626"],"doi":["10.1371/journal.pone.0155454"],"title":["Exposure to Aedes aegypti Bites Induces a Mixed-Type Allergic Response following Salivary Antigens Challenge in Mice."],"authorString":["Barros MS, Gomes E, Gueroni DI, Ramos AD, Mirotti L, Florsheim E, Bizzarro B, Lino CN, Maciel C, Lino-Dos-Santos-Franco A, Tavares-de-Lima W, Capurro ML, Russo M, Sá-Nunes A."],"authorList":[{"author":[{"fullName":["Barros MS"],"firstName":["Michele S"],"lastName":["Barros"],"initials":["MS"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Gomes E"],"firstName":["Eliane"],"lastName":["Gomes"],"initials":["E"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Gueroni DI"],"firstName":["Daniele I"],"lastName":["Gueroni"],"initials":["DI"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Ramos AD"],"firstName":["Anderson D"],"lastName":["Ramos"],"initials":["AD"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Mirotti L"],"firstName":["Luciana"],"lastName":["Mirotti"],"initials":["L"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Florsheim E"],"firstName":["Esther"],"lastName":["Florsheim"],"initials":["E"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Bizzarro B"],"firstName":["Bruna"],"lastName":["Bizzarro"],"initials":["B"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Lino CN"],"firstName":["Ciro N R"],"lastName":["Lino"],"initials":["CN"],"affiliation":["Institute of Immunology, Hannover Medical School, Hannover, Germany."]},{"fullName":["Maciel C"],"firstName":["Ceres"],"lastName":["Maciel"],"initials":["C"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Lino-Dos-Santos-Franco A"],"firstName":["Adriana"],"lastName":["Lino-Dos-Santos-Franco"],"initials":["A"],"affiliation":["Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), São Paulo - SP, Brazil."]},{"fullName":["Tavares-de-Lima W"],"firstName":["Wothan"],"lastName":["Tavares-de-Lima"],"initials":["W"],"affiliation":["Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Capurro ML"],"firstName":["Margareth L"],"lastName":["Capurro"],"initials":["ML"],"affiliation":["National Institute of Science and Technology in Molecular Entomology, National Council of Scientific and Technological Development (INCT-EM/CNPq), Rio de Janeiro - RJ, Brazil."]},{"fullName":["Russo M"],"firstName":["Momtchilo"],"lastName":["Russo"],"initials":["M"],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."]},{"fullName":["Sá-Nunes A"],"firstName":["Anderson"],"lastName":["Sá-Nunes"],"initials":["A"],"authorId":[{"_":"0000-0002-1859-4973","$":{"type":"ORCID"}}],"affiliation":["National Institute of Science and Technology in Molecular Entomology, National Council of Scientific and Technological Development (INCT-EM/CNPq), Rio de Janeiro - RJ, Brazil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1859-4973","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["11"],"journalIssueId":["2403348"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0155454"],"abstractText":["Classical studies have shown that Aedes aegypti salivary secretion is responsible for the sensitization to mosquito bites and many of the components present in saliva are immunogenic and capable of inducing an intense immune response. Therefore, we have characterized a murine model of adjuvant-free systemic allergy induced by natural exposure to mosquito bites. BALB/c mice were sensitized by exposure to A. aegypti mosquito bites and intranasally challenged with phosphate-buffered saline only or the mosquito's salivary gland extract (SGE). Blood, bronchoalveolar lavage (BAL) and lung were collected and evaluated for cellularity, histopathological analyses, cytokines and antibody determination. Respiratory pattern was analyzed by Penh measurements and tracheal segments were obtained to study in vitro reactivity to methacholine. BAL recovered from sensitized mice following challenge with SGE showed an increased number of eosinophils and Th2 cytokines such as IL-4, IL-5 and IL-13. Peribronchoalveolar eosinophil infiltration, mucus and collagen were also observed in lung parenchyma of sensitized mice, suggesting the development of a typical Th2 response. However, the antibody profile in serum of these mice evidenced a mixed-type response with presence of both, IgG1/IgE (Th2-related) and IgG2a (Th1-related) isotypes. In addition, changes in breathing pattern and tracheal reactivity to methacholine were not found. Taken together, our results show that A. aegypti bites trigger an atypical allergic reaction, with some classical cellular and soluble Th2 components in the lung, but also systemic Th1 and Th2 antibody isotypes and no change in either the respiratory pattern or the trachea responsiveness to agonist."],"affiliation":["Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo - SP, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4874626?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4874626"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0155454"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-21"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-20"],"firstPublicationDate":["2016-05-20"],"cprojectID":"PMC4874626"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnreZ_8VqgcuJwvii","_score":1,"_source":{"id":["27120007"],"source":["MED"],"pmid":["27120007"],"pmcid":["PMC4878304"],"doi":["10.1590/0074-02760160004"],"title":["Phylogenetic analyses of chikungunya virus among travelers in Rio de Janeiro, Brazil, 2014-2015."],"authorString":["Conteville LC, Zanella L, Marín MA, Filippis AM, Nogueira RM, Vicente AC, Mendonça MC."],"authorList":[{"author":[{"fullName":["Conteville LC"],"firstName":["Liliane Costa"],"lastName":["Conteville"],"initials":["LC"],"affiliation":["Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Zanella L"],"firstName":["Louise"],"lastName":["Zanella"],"initials":["L"],"affiliation":["Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Marín MA"],"firstName":["Michel Abanto"],"lastName":["Marín"],"initials":["MA"],"affiliation":["Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Filippis AM"],"firstName":["Ana Maria Bispo de"],"lastName":["Filippis"],"initials":["AM"],"affiliation":["Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Nogueira RM"],"firstName":["Rita Maria Ribeiro"],"lastName":["Nogueira"],"initials":["RM"],"affiliation":["Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Vicente AC"],"firstName":["Ana Carolina Paulo"],"lastName":["Vicente"],"initials":["AC"],"affiliation":["Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Mendonça MC"],"firstName":["Marcos César Lima de"],"lastName":["Mendonça"],"initials":["MC"],"affiliation":["Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."]}]}],"journalInfo":[{"issue":["5"],"volume":["111"],"journalIssueId":["2412036"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["347-348"],"abstractText":["Chikungunya virus (CHIKV) is a mosquito-borne pathogen that emerged in Brazil by late 2014. In the country, two CHIKV foci characterized by the East/Central/South Africa and Asian genotypes, were established in North and Northeast regions. We characterized, by phylogenetic analyses of full and partial genomes, CHIKV from Rio de Janeiro state (2014-2015). These CHIKV strains belong to the Asian genotype, which is the determinant of the current Northern Brazilian focus, even though the genome sequence presents particular single nucleotide variations. This study provides the first genetic characterisation of CHIKV in Rio de Janeiro and highlights the potential impact of human mobility in the spread of an arthropod-borne virus."],"affiliation":["Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878304?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878304"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160004"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-26"],"dateOfRevision":["2016-05-26"],"electronicPublicationDate":["2016-03-29"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4878304"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrerJ9hGurOzZIaf","_score":1,"_source":{"id":["27303723"],"source":["MED"],"pmid":["27303723"],"pmcid":["PMC4894681"],"doi":["10.1128/msphere.00069-16"],"title":["Zika Virus Focuses the Gain-of-Function Debate."],"authorString":["Imperiale MJ, Casadevall A."],"authorList":[{"author":[{"fullName":["Imperiale MJ"],"firstName":["Michael J"],"lastName":["Imperiale"],"initials":["MJ"],"affiliation":["Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA."]},{"fullName":["Casadevall A"],"firstName":["Arturo"],"lastName":["Casadevall"],"initials":["A"],"affiliation":["Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA."]}]}],"journalInfo":[{"issue":["2"],"volume":["1"],"journalIssueId":["2420017"],"dateOfPublication":["2016 Mar-Apr"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["mSphere"],"ISOAbbreviation":["mSphere"],"medlineAbbreviation":["mSphere"],"NLMid":["101674533"],"ESSN":["2379-5042"]}]}],"pubYear":["2016"],"affiliation":["Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4894681?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4894681"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mSphere.00069-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-15"],"dateOfCreation":["2016-06-15"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-04-06"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4894681"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnre9hOUS2orL1IQs","_score":1,"_source":{"id":["27274587"],"source":["MED"],"pmid":["27274587"],"pmcid":["PMC4890210"],"doi":["10.2471/blt.16.000616"],"title":["In this month's Bulletin."],"journalInfo":[{"issue":["6"],"volume":["94"],"journalIssueId":["2415566"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["405"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890210?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4890210"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.000616"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-10"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4890210"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfNJ9hGurOzZIag","_score":1,"_source":{"id":["27174054"],"source":["MED"],"pmid":["27174054"],"pmcid":["PMC4897185"],"doi":["10.1038/cr.2016.58"],"title":["Vertical transmission of Zika virus targeting the radial glial cells affects cortex development of offspring mice."],"authorString":["Wu KY, Zuo GL, Li XF, Ye Q, Deng YQ, Huang XY, Cao WC, Qin CF, Luo ZG."],"authorList":[{"author":[{"fullName":["Wu KY"],"firstName":["Kong-Yan"],"lastName":["Wu"],"initials":["KY"],"affiliation":["Institute of Neuroscience, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China."]},{"fullName":["Zuo GL"],"firstName":["Guo-Long"],"lastName":["Zuo"],"initials":["GL"],"affiliation":["Chinese Academy of Sciences University, Beijing, China."]},{"fullName":["Li XF"],"firstName":["Xiao-Feng"],"lastName":["Li"],"initials":["XF"],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Ye Q"],"firstName":["Qing"],"lastName":["Ye"],"initials":["Q"],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Deng YQ"],"firstName":["Yong-Qiang"],"lastName":["Deng"],"initials":["YQ"],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Huang XY"],"firstName":["Xing-Yao"],"lastName":["Huang"],"initials":["XY"],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Cao WC"],"firstName":["Wu-Chun"],"lastName":["Cao"],"initials":["WC"],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Qin CF"],"firstName":["Cheng-Feng"],"lastName":["Qin"],"initials":["CF"],"authorId":[{"_":"0000-0002-0632-2807","$":{"type":"ORCID"}}],"affiliation":["Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China."]},{"fullName":["Luo ZG"],"firstName":["Zhen-Ge"],"lastName":["Luo"],"initials":["ZG"],"affiliation":["ShanghaiTech University, Shanghai, China."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0632-2807","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["26"],"journalIssueId":["2415413"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Cell research"],"ISOAbbreviation":["Cell Res."],"medlineAbbreviation":["Cell Res"],"NLMid":["9425763"],"ISSN":["1001-0602"],"ESSN":["1748-7838"]}]}],"pubYear":["2016"],"pageInfo":["645-654"],"abstractText":["The recent Zika virus (ZIKV) epidemic in Latin America coincided with a marked increase in microcephaly in newborns. However, the causal link between maternal ZIKV infection and malformation of the fetal brain has not been firmly established. Here we show a vertical transmission of ZIKV in mice and a marked effect on fetal brain development. We found that intraperitoneal (i.p.) injection of a contemporary ZIKV strain in pregnant mice led to the infection of radial glia cells (RGs) of dorsal ventricular zone of the fetuses, the primary neural progenitors responsible for cortex development, and caused a marked reduction of these cortex founder cells in the fetuses. Interestingly, the infected fetal mice exhibited a reduced cavity of lateral ventricles and a discernable decrease in surface areas of the cortex. This study thus supports the conclusion that vertically transmitted ZIKV affects fetal brain development and provides a valuable animal model for the evaluation of potential therapeutic or preventative strategies."],"affiliation":["Institute of Neuroscience, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4897185?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4897185"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/cr.2016.58"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-06-03"],"dateOfRevision":["2016-06-22"],"electronicPublicationDate":["2016-05-13"],"firstPublicationDate":["2016-05-13"],"cprojectID":"PMC4897185"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrim_8VqgcuJwvip","_score":1,"_source":{"id":["27227829"],"source":["MED"],"pmid":["27227829"],"pmcid":["PMC4881945"],"doi":["10.1371/journal.pntd.0004588"],"title":["Quantifying the Epidemiological Impact of Vector Control on Dengue."],"authorString":["Reiner RC, Achee N, Barrera R, Burkot TR, Chadee DD, Devine GJ, Endy T, Gubler D, Hombach J, Kleinschmidt I, Lenhart A, Lindsay SW, Longini I, Mondy M, Morrison AC, Perkins TA, Vazquez-Prokopec G, Reiter P, Ritchie SA, Smith DL, Strickman D, Scott TW."],"authorList":[{"author":[{"fullName":["Reiner RC Jr"],"firstName":["Robert C"],"lastName":["Reiner"],"initials":["RC"],"affiliation":["Fogarty International Center, National Institutes of Health, Bethesda, Maryland, United States of America."]},{"fullName":["Achee N"],"firstName":["Nicole"],"lastName":["Achee"],"initials":["N"],"affiliation":["Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, United States of America."]},{"fullName":["Barrera R"],"firstName":["Roberto"],"lastName":["Barrera"],"initials":["R"],"affiliation":["Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico."]},{"fullName":["Burkot TR"],"firstName":["Thomas R"],"lastName":["Burkot"],"initials":["TR"],"affiliation":["Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Queensland, Australia."]},{"fullName":["Chadee DD"],"firstName":["Dave D"],"lastName":["Chadee"],"initials":["DD"],"affiliation":["Department of Life Sciences, Faculty of Science and Agriculture, The University of the West Indies, St. Augustine Campus, St. Augustine, Trinidad and Tobago."]},{"fullName":["Devine GJ"],"firstName":["Gregor J"],"lastName":["Devine"],"initials":["GJ"],"affiliation":["QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia."]},{"fullName":["Endy T"],"firstName":["Timothy"],"lastName":["Endy"],"initials":["T"],"affiliation":["Department of Medicine, Upstate Medical University of New York, Syracuse, New York, United States of America."]},{"fullName":["Gubler D"],"firstName":["Duane"],"lastName":["Gubler"],"initials":["D"],"affiliation":["Signature Research Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore."]},{"fullName":["Hombach J"],"firstName":["Joachim"],"lastName":["Hombach"],"initials":["J"],"affiliation":["Initiative for Vaccine Research, World Health Organization, Geneva, Switzerland."]},{"fullName":["Kleinschmidt I"],"firstName":["Immo"],"lastName":["Kleinschmidt"],"initials":["I"],"affiliation":["Department of Pathology, School of Health Sciences, University of Witwatersrand, Johannesburg, South Africa."]},{"fullName":["Lenhart A"],"firstName":["Audrey"],"lastName":["Lenhart"],"initials":["A"],"affiliation":["Centers for Disease Control and Prevention, Center for Global Health/Division of Parasitic Diseases and Malaria/Entomology Branch, Atlanta, Georgia, United States of America."]},{"fullName":["Lindsay SW"],"firstName":["Steven W"],"lastName":["Lindsay"],"initials":["SW"],"affiliation":["School of Biological and Biomedical Sciences, Durham University, Durham, United Kingdom."]},{"fullName":["Longini I"],"firstName":["Ira"],"lastName":["Longini"],"initials":["I"],"affiliation":["Department of Biostatistics, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Mondy M"],"firstName":["Mathias"],"lastName":["Mondy"],"initials":["M"],"affiliation":["Innovative Vector Control Consortium."]},{"fullName":["Morrison AC"],"firstName":["Amy C"],"lastName":["Morrison"],"initials":["AC"],"affiliation":["Department of Entomology and Nematology, University of California, Davis, California, United States of America."]},{"fullName":["Perkins TA"],"firstName":["T Alex"],"lastName":["Perkins"],"initials":["TA"],"affiliation":["Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, Indiana, United States of America."]},{"fullName":["Vazquez-Prokopec G"],"firstName":["Gonzalo"],"lastName":["Vazquez-Prokopec"],"initials":["G"],"affiliation":["Department of Environmental Studies, Emory University, Atlanta, Georgia, United States of America."]},{"fullName":["Reiter P"],"firstName":["Paul"],"lastName":["Reiter"],"initials":["P"],"affiliation":["Department of Medical Entomology, Institut Pasteur, Paris, France."]},{"fullName":["Ritchie SA"],"firstName":["Scott A"],"lastName":["Ritchie"],"initials":["SA"],"affiliation":["College of Public Health, Medical, and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns, Queensland, Australia."]},{"fullName":["Smith DL"],"firstName":["David L"],"lastName":["Smith"],"initials":["DL"],"affiliation":["Institute for Health Metrics and Evaluation, University of Washington, Seattle, Washington, United States of America."]},{"fullName":["Strickman D"],"firstName":["Daniel"],"lastName":["Strickman"],"initials":["D"],"affiliation":["Bill & Melinda Gates Foundation, Seattle, Washington, United States of America."]},{"fullName":["Scott TW"],"firstName":["Thomas W"],"lastName":["Scott"],"initials":["TW"],"affiliation":["Department of Entomology and Nematology, University of California, Davis, California, United States of America."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004588"],"affiliation":["Fogarty International Center, National Institutes of Health, Bethesda, Maryland, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4881945?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4881945"]},{"availability":["Subscription 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlg_8VqgcuJwvi4","_score":1,"_source":{"id":["27222466"],"source":["MED"],"pmid":["27222466"],"pmcid":["PMC4895102"],"doi":["10.1128/mbio.00314-16"],"title":["Transcriptome Analysis Reveals a Signature Profile for Tick-Borne Flavivirus Persistence in HEK 293T Cells."],"authorString":["Mlera L, Lam J, Offerdahl DK, Martens C, Sturdevant D, Turner CV, Porcella SF, Bloom ME."],"authorList":[{"author":[{"fullName":["Mlera L"],"firstName":["Luwanika"],"lastName":["Mlera"],"initials":["L"],"affiliation":["Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA Luwanika.Mlera@nih.gov."]},{"fullName":["Lam J"],"firstName":["Jennifer"],"lastName":["Lam"],"initials":["J"],"affiliation":["Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA."]},{"fullName":["Offerdahl DK"],"firstName":["Danielle K"],"lastName":["Offerdahl"],"initials":["DK"],"affiliation":["Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA."]},{"fullName":["Martens C"],"firstName":["Craig"],"lastName":["Martens"],"initials":["C"],"affiliation":["Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA."]},{"fullName":["Sturdevant D"],"firstName":["Daniel"],"lastName":["Sturdevant"],"initials":["D"],"affiliation":["Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA."]},{"fullName":["Turner CV"],"firstName":["Charles V"],"lastName":["Turner"],"initials":["CV"],"affiliation":["Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA."]},{"fullName":["Porcella SF"],"firstName":["Stephen F"],"lastName":["Porcella"],"initials":["SF"],"affiliation":["Genomics Unit, Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA."]},{"fullName":["Bloom ME"],"firstName":["Marshall E"],"lastName":["Bloom"],"initials":["ME"],"affiliation":["Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA."]}]}],"journalInfo":[{"issue":["3"],"volume":["7"],"journalIssueId":["2404410"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"abstractText":["Tick-borne flaviviruses (TBFVs) cause febrile illnesses, which may progress to severe encephalitis and/or death in humans globally. Most people who recover from severe acute disease suffer from debilitating neurological sequelae, which may be due to viral persistence, infection-induced neurological cell damage, host response, or some combination of these. Acute TBFV infection of human embryonic kidney (HEK) 293T cells in vitro results in the death of >95% of infected cells by day 5. However, replacing cell growth medium allows surviving cells to repopulate and become persistently infected for extended periods of time. The mechanisms responsible for initiation and maintenance of viral persistence remain vague. We subjected the HEK 293T cell transcriptome to deep sequencing to identify genes differentially expressed during acute infection and persistent infection. A total of 451 genes showed unique significant differential expression levels in persistently infected cells relative to the acute phase of infection. Ingenuity Pathway Analysis results suggested that the expression of prosurvival oncogenes AKT2 and ERBB2 was upregulated in persistently infected cells, whereas proapoptotic genes, such as Bad and the beta interferon 1 (IFN-β1) gene, were downregulated. Genes encoding antiviral cytokines such as the CCL5, tumor necrosis factor alpha (TNF-α), and CXCL10 genes were upregulated during the acute phase, but the same genes were relatively quiescent in persistently infected cells. Exogenous induction of apoptosis demonstrated that persistently infected cells were resistant to apoptosis in a dose-dependent manner. In summary, the differential transcriptome profiles of acute-phase compared to persistently infected HEK 293T cells demonstrated an evasion of apoptosis, which may be critical for a chronic TBFV infection state. These results provide a basis for further study of the mechanisms of TBFV persistence.Tick-borne flaviviruses (TBFVs) cause life-threatening encephalitic disease in humans worldwide. Some people who recover from severe disease may suffer prolonged neurological symptoms due to either virus- or host response-induced cell damage or a combination of the two that are linked to viral persistence. By examining the genes that are significantly differentially expressed in acute TBFV infection versus persistent TBFV infection, we may be able to find the molecular basis of viral persistence. Here we used deep sequencing of the host cell transcriptome to discover that the expression levels of prosurvival genes were upregulated in persistently infected cells relative to acute TBFV infections whereas the expression levels of genes that promote programmed cell death were downregulated. In addition, persistently infected cells were also resistant to exogenous chemical induction of cell death, in a dose-dependent manner, compared to uninfected cells. Our results pave the way for further studies aimed at understanding the precise mechanisms of TBFV persistence."],"affiliation":["Laboratory of Virology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA Luwanika.Mlera@nih.gov."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4895102?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4895102"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.00314-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-25"],"dateOfRevision":["2016-06-22"],"electronicPublicationDate":["2016-05-24"],"firstPublicationDate":["2016-05-24"],"cprojectID":"PMC4895102"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlsJ9hGurOzZIa3","_score":1,"_source":{"id":["27366586"],"source":["MED"],"pmid":["27366586"],"pmcid":["PMC4922743"],"doi":["10.1371/currents.outbreaks.f14b2217c902f453d9320a43a35b9583"],"title":["Using Phenomenological Models to Characterize Transmissibility and Forecast Patterns and Final Burden of Zika Epidemics."],"authorString":["Chowell G, Hincapie-Palacio D, Ospina J, Pell B, Tariq A, Dahal S, Moghadas S, Smirnova A, Simonsen L, Viboud C."],"authorList":[{"author":[{"fullName":["Chowell G"],"firstName":["Gerardo"],"lastName":["Chowell"],"initials":["G"],"affiliation":["Division of Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA; Mathematical, Computational & Modeling Sciences Center, School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, USA."]},{"fullName":["Hincapie-Palacio D"],"firstName":["Doracelly"],"lastName":["Hincapie-Palacio"],"initials":["D"]},{"fullName":["Ospina J"],"firstName":["Juan"],"lastName":["Ospina"],"initials":["J"],"affiliation":["Logic and Computation Group, EAFIT University, Medellin, Antioquia, Colombia."]},{"fullName":["Pell B"],"firstName":["Bruce"],"lastName":["Pell"],"initials":["B"]},{"fullName":["Tariq A"],"firstName":["Amna"],"lastName":["Tariq"],"initials":["A"],"affiliation":["School of Public Health, Georgia State University, Atlanta, Georgia, USA."]},{"fullName":["Dahal S"],"firstName":["Sushma"],"lastName":["Dahal"],"initials":["S"],"affiliation":["School of Public Health, Georgia State University, Atlanta, Georgia, USA."]},{"fullName":["Moghadas S"],"firstName":["Seyed"],"lastName":["Moghadas"],"initials":["S"],"affiliation":["Agent Based Modelling Laboratory, York University, Toronto, Canada."]},{"fullName":["Smirnova A"],"firstName":["Alexandra"],"lastName":["Smirnova"],"initials":["A"],"affiliation":["Department of Mathematics and Statistics, Georgia State University, Atlanta, Georgia, USA."]},{"fullName":["Simonsen L"],"firstName":["Lone"],"lastName":["Simonsen"],"initials":["L"],"affiliation":["Department of Public Health, University of Copenhagen, Copenhagen, Denmark."]},{"fullName":["Viboud C"],"firstName":["Cécile"],"lastName":["Viboud"],"initials":["C"],"affiliation":["Division of International Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["The World Health Organization declared the ongoing Zika virus (ZIKV) epidemic in the Americas a Public Health Emergency of International Concern on February 1, 2016. ZIKV disease in humans is characterized by a \"dengue-like\" syndrome including febrile illness and rash. However, ZIKV infection in early pregnancy has been associated with severe birth defects, including microcephaly and other developmental issues. Mechanistic models of disease transmission can be used to forecast trajectories and likely disease burden but are currently hampered by substantial uncertainty on the epidemiology of the disease (e.g., the role of asymptomatic transmission, generation interval, incubation period, and key drivers). When insight is limited, phenomenological models provide a starting point for estimation of key transmission parameters, such as the reproduction number, and forecasts of epidemic impact.We obtained daily counts of suspected Zika cases by date of symptoms onset from the Secretary of Health of Antioquia, Colombia during January-April 2016. We calibrated the generalized Richards model, a phenomenological model that accommodates a variety of early exponential and sub-exponential growth kinetics, against the early epidemic trajectory and generated predictions of epidemic size. The reproduction number was estimated by applying the renewal equation to incident cases simulated from the fitted generalized-growth model and assuming gamma or exponentially-distributed generation intervals derived from the literature. We estimated the reproduction number for an increasing duration of the epidemic growth phase.The reproduction number rapidly declined from 10.3 (95% CI: 8.3, 12.4) in the first disease generation to 2.2 (95% CI: 1.9, 2.8) in the second disease generation, assuming a gamma-distributed generation interval with the mean of 14 days and standard deviation of 2 days. The generalized-Richards model outperformed the logistic growth model and provided forecasts within 22% of the actual epidemic size based on an assessment 30 days into the epidemic, with the epidemic peaking on day 36.Phenomenological models represent promising tools to generate early forecasts of epidemic impact particularly in the context of substantial uncertainty in epidemiological parameters. Our findings underscore the need to treat the reproduction number as a dynamic quantity even during the early growth phase, and emphasize the sensitivity of reproduction number estimates to assumptions on the generation interval distribution."],"affiliation":["Division of Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA; Mathematical, Computational & Modeling Sciences Center, School of Human Evolution and Social Change, Arizona State University, Tempe, Arizona, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4922743"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.f14b2217c902f453d9320a43a35b9583"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-01"],"dateOfCreation":["2016-07-01"],"electronicPublicationDate":["2016-05-31"],"firstPublicationDate":["2016-05-31"],"cprojectID":"PMC4922743"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrqf_8VqgcuJwvjO","_score":1,"_source":{"id":["27341420"],"source":["MED"],"pmid":["27341420"],"pmcid":["PMC4920388"],"doi":["10.1371/journal.pntd.0004816"],"title":["Isolation of Infective Zika Virus from Urine and Saliva of Patients in Brazil."],"authorString":["Bonaldo MC, Ribeiro IP, Lima NS, Dos Santos AA, Menezes LS, da Cruz SO, de Mello IS, Furtado ND, de Moura EE, Damasceno L, da Silva KA, de Castro MG, Gerber AL, de Almeida LG, Lourenço-de-Oliveira R, Vasconcelos AT, Brasil P."],"authorList":[{"author":[{"fullName":["Bonaldo MC"],"firstName":["Myrna C"],"lastName":["Bonaldo"],"initials":["MC"],"authorId":[{"_":"0000-0001-9899-3386","$":{"type":"ORCID"}}],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Ribeiro IP"],"firstName":["Ieda P"],"lastName":["Ribeiro"],"initials":["IP"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Lima NS"],"firstName":["Noemia S"],"lastName":["Lima"],"initials":["NS"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Dos Santos AA"],"firstName":["Alexandre A C"],"lastName":["Dos Santos"],"initials":["AA"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Menezes LS"],"firstName":["Lidiane S R"],"lastName":["Menezes"],"initials":["LS"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["da Cruz SO"],"firstName":["Stephanie O D"],"lastName":["da Cruz"],"initials":["SO"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["de Mello IS"],"firstName":["Iasmim S"],"lastName":["de Mello"],"initials":["IS"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Furtado ND"],"firstName":["Nathália D"],"lastName":["Furtado"],"initials":["ND"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["de Moura EE"],"firstName":["Elaine E"],"lastName":["de Moura"],"initials":["EE"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Damasceno L"],"firstName":["Luana"],"lastName":["Damasceno"],"initials":["L"],"affiliation":["Laboratório de Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["da Silva KA"],"firstName":["Kely A B"],"lastName":["da Silva"],"initials":["KA"],"affiliation":["Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["de Castro MG"],"firstName":["Marcia G"],"lastName":["de Castro"],"initials":["MG"],"affiliation":["Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Gerber AL"],"firstName":["Alexandra L"],"lastName":["Gerber"],"initials":["AL"],"affiliation":["Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil."]},{"fullName":["de Almeida LG"],"firstName":["Luiz G P"],"lastName":["de Almeida"],"initials":["LG"],"affiliation":["Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil."]},{"fullName":["Lourenço-de-Oliveira R"],"firstName":["Ricardo"],"lastName":["Lourenço-de-Oliveira"],"initials":["R"],"affiliation":["Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."]},{"fullName":["Vasconcelos AT"],"firstName":["Ana Tereza R"],"lastName":["Vasconcelos"],"initials":["AT"],"affiliation":["Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil."]},{"fullName":["Brasil P"],"firstName":["Patrícia"],"lastName":["Brasil"],"initials":["P"],"affiliation":["Laboratório de Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9899-3386","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004816"],"abstractText":["Zika virus (ZIKV) is an emergent threat provoking a worldwide explosive outbreak. Since January 2015, 41 countries reported autochthonous cases. In Brazil, an increase in Guillain-Barré syndrome and microcephaly cases was linked to ZIKV infections. A recent report describing low experimental transmission efficiency of its main putative vector, Ae. aegypti, in conjunction with apparent sexual transmission notifications, prompted the investigation of other potential sources of viral dissemination. Urine and saliva have been previously established as useful tools in ZIKV diagnosis. Here, we described the presence and isolation of infectious ZIKV particles from saliva and urine of acute phase patients in the Rio de Janeiro state, Brazil.Nine urine and five saliva samples from nine patients from Rio de Janeiro presenting rash and other typical Zika acute phase symptoms were inoculated in Vero cell culture and submitted to specific ZIKV RNA detection and quantification through, respectively, NAT-Zika, RT-PCR and RT-qPCR. Two ZIKV isolates were achieved, one from urine and one from saliva specimens. ZIKV nucleic acid was identified by all methods in four patients. Whenever both urine and saliva samples were available from the same patient, urine viral loads were higher, corroborating the general sense that it is a better source for ZIKV molecular diagnostic. In spite of this, from the two isolated strains, each from one patient, only one derived from urine, suggesting that other factors, like the acidic nature of this fluid, might interfere with virion infectivity. The complete genome of both ZIKV isolates was obtained. Phylogenetic analysis revealed similarity with strains previously isolated during the South America outbreak.The detection of infectious ZIKV particles in urine and saliva of patients during the acute phase may represent a critical factor in the spread of virus. The epidemiological relevance of this finding, regarding the contribution of alternative non-vectorial ZIKV transmission routes, needs further investigation."],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920388?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920388"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004816"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-25"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-06-24"],"firstPublicationDate":["2016-06-24"],"cprojectID":"PMC4920388"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrqzJ9hGurOzZIbP","_score":1,"_source":{"id":["27419186"],"source":["MED"],"pmid":["27419186"],"pmcid":["PMC4942759"],"doi":["10.1093/ofid/ofw122"],"title":["Defining Clinical Excellence in Adult Infectious Disease Practice."],"authorString":["Chida NM, Ghanem KG, Auwaerter PG, Wright SM, Melia MT."],"authorList":[{"author":[{"fullName":["Chida NM"],"firstName":["Natasha M"],"lastName":["Chida"],"initials":["NM"],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."]},{"fullName":["Ghanem KG"],"firstName":["Khalil G"],"lastName":["Ghanem"],"initials":["KG"],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."]},{"fullName":["Auwaerter PG"],"firstName":["Paul G"],"lastName":["Auwaerter"],"initials":["PG"],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."]},{"fullName":["Wright SM"],"firstName":["Scott M"],"lastName":["Wright"],"initials":["SM"],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."]},{"fullName":["Melia MT"],"firstName":["Michael T"],"lastName":["Melia"],"initials":["MT"],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."]}]}],"journalInfo":[{"issue":["3"],"volume":["3"],"journalIssueId":["2426394"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Open forum infectious diseases"],"ISOAbbreviation":["Open Forum Infect Dis"],"medlineAbbreviation":["Open Forum Infect Dis"],"NLMid":["101637045"],"ESSN":["2328-8957"]}]}],"pubYear":["2016"],"pageInfo":["ofw122"],"abstractText":["Clinical excellence should be recognized, particularly in the current climate that appropriately prioritizes relationship-centered care. In order to develop a recognition model, a definition of clinical excellence must be created and agreed upon. A paradigm recently suggested by C. Christmas describes clinical excellence through the following domains: diagnostic acumen, professionalism and humanism, communication and interpersonal skills, skillful negotiation of the healthcare system, knowledge, taking a scholarly approach to clinical practice, and having passion for clinical medicine. This work references examples of infectious disease (ID) clinical excellence across Christmas' domains and, in doing so, both examines how the definition of clinical excellence applies to ID practice and highlights the importance of ID physicians. Emphasizing such aspirational standards may not only inspire trainees and practicing physicians to pursue their own fulfilling clinical ID careers, it may also encourage health systems to fully value outstanding ID physicians who labor tirelessly to provide patients with exceptional care."],"affiliation":["Department of Medicine , Johns Hopkins University School of Medicine , Baltimore, Maryland."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Infectious disease","Clinician","Clinical Excellence"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942759?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4942759"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/ofid/ofw122"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-15"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4942759"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrrXJ9hGurOzZIbQ","_score":1,"_source":{"id":["27419175"],"source":["MED"],"pmid":["27419175"],"pmcid":["PMC4943532"],"doi":["10.1093/ofid/ofw103"],"title":["Time to Empower Release of Insects Carrying a Dominant Lethal and Wolbachia Against Zika."],"authorString":["Dickens BL, Yang J, Cook AR, Carrasco LR."],"authorList":[{"author":[{"fullName":["Dickens BL"],"firstName":["Borame L"],"lastName":["Dickens"],"initials":["BL"],"affiliation":["Department of Biological Sciences , National University of Singapore."]},{"fullName":["Yang J"],"firstName":["Jie"],"lastName":["Yang"],"initials":["J"],"affiliation":["Saw Swee Hock School of Public Health , National University of Singapore."]},{"fullName":["Cook AR"],"firstName":["Alex R"],"lastName":["Cook"],"initials":["AR"],"affiliation":["Saw Swee Hock School of Public Health , National University of Singapore."]},{"fullName":["Carrasco LR"],"firstName":["Luis R"],"lastName":["Carrasco"],"initials":["LR"],"affiliation":["Department of Biological Sciences , National University of Singapore."]}]}],"journalInfo":[{"issue":["2"],"volume":["3"],"journalIssueId":["2430619"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Open forum infectious diseases"],"ISOAbbreviation":["Open Forum Infect Dis"],"medlineAbbreviation":["Open Forum Infect Dis"],"NLMid":["101637045"],"ESSN":["2328-8957"]}]}],"pubYear":["2016"],"pageInfo":["ofw103"],"abstractText":["RIDL (release of insects with dominant lethality) and Wolbachia are 2 potentially powerful tools in the fight against Zika, but their technological advancement is being hampered by policy barriers. In this study, we discuss what could be done to overcome these regulatory deadlocks."],"affiliation":["Department of Biological Sciences , National University of Singapore."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Wolbachia","Health Policy","Vector Control","Ridl","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4943532?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4943532"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/ofid/ofw103"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-15"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-05-19"],"firstPublicationDate":["2016-04-01"],"cprojectID":"PMC4943532"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrrxhOUS2orL1IRg","_score":1,"_source":{"id":["27416870"],"source":["MED"],"pmid":["27416870"],"pmcid":["PMC4945905"],"doi":["10.1038/srep29835"],"title":["Tyrosine Hydroxylase is crucial for maintaining pupal tanning and immunity in Anopheles sinensis."],"authorString":["Qiao L, Du M, Liang X, Hao Y, He X, Si F, Mei T, Chen B."],"authorList":[{"author":[{"fullName":["Qiao L"],"firstName":["Liang"],"lastName":["Qiao"],"initials":["L"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Du M"],"firstName":["Minghui"],"lastName":["Du"],"initials":["M"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Liang X"],"firstName":["Xin"],"lastName":["Liang"],"initials":["X"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Hao Y"],"firstName":["Youjin"],"lastName":["Hao"],"initials":["Y"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["He X"],"firstName":["Xiu"],"lastName":["He"],"initials":["X"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Si F"],"firstName":["Fengling"],"lastName":["Si"],"initials":["F"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Mei T"],"firstName":["Ting"],"lastName":["Mei"],"initials":["T"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]},{"fullName":["Chen B"],"firstName":["Bin"],"lastName":["Chen"],"initials":["B"],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["29835"],"abstractText":["Tyrosine hydroxylase (TH), the initial enzyme in the melanin pathway, catalyzes tyrosine conversion into Dopa. Although expression and regulation of TH have been shown to affect cuticle pigmentation in insects, no direct functional studies to date have focused on the specific physiological processes involving the enzyme during mosquito development. In the current study, silencing of AsTH during the time period of continuous high expression in Anopheles sinensis pupae led to significant impairment of cuticle tanning and thickness, imposing a severe obstacle to eclosion in adults. Meanwhile, deficiency of melanin in interference individuals led to suppression of melanization, compared to control individuals. Consequently, the ability to defend exogenous microorganisms declined sharply. Accompanying down-regulation of the basal expression of five antimicrobial peptide genes resulted in further significant weakening of immunity. TH homologs as well as the composition of upstream transcription factor binding sites at the pupal stage are highly conserved in the Anopheles genus, implying that the TH-mediated functions are crucial in Anopheles. The collective evidence strongly suggests that TH is essential for Anopheles pupae tanning and immunity and provides a reference for further studies to validate the utility of the key genes involved in the melanization pathway in controlling mosquito development."],"affiliation":["Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, 401331, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4945905?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4945905"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep29835"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-27"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"cprojectID":"PMC4945905"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrvIhOUS2orL1IRt","_score":1,"_source":{"id":["27399756"],"source":["MED"],"pmid":["27399756"],"pmcid":["PMC4962230"],"doi":["10.3390/ijerph13070689"],"title":["Surveillance of Mosquitoes and Selected Arthropod-Borne Viruses in the Context of Milan EXPO 2015."],"authorString":["Chiari M, Calzolari M, Prosperi A, Perulli S, Faccin F, Avisani D, Cerioli M, Zanoni M, Tironi M, Bertoletti M, Defilippo F, Moreno A, Farioli M, Piatti A, Dottori M, Lelli D, Lavazza A."],"authorList":[{"author":[{"fullName":["Chiari M"],"firstName":["Mario"],"lastName":["Chiari"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. mario.chiari@izsler.it."]},{"fullName":["Calzolari M"],"firstName":["Mattia"],"lastName":["Calzolari"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. mattia.calzolari@izsler.it."]},{"fullName":["Prosperi A"],"firstName":["Alice"],"lastName":["Prosperi"],"initials":["A"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. alice.prosperi@izsler.it."]},{"fullName":["Perulli S"],"firstName":["Simona"],"lastName":["Perulli"],"initials":["S"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. simona.perulli@izsler.it."]},{"fullName":["Faccin F"],"firstName":["Francesca"],"lastName":["Faccin"],"initials":["F"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. francesca.faccin@izsler.it."]},{"fullName":["Avisani D"],"firstName":["Dominga"],"lastName":["Avisani"],"initials":["D"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. dominga.avisani@izsler.it."]},{"fullName":["Cerioli M"],"firstName":["Monica"],"lastName":["Cerioli"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. monicapierangela.cerioli@izsler.it."]},{"fullName":["Zanoni M"],"firstName":["Mariagrazia"],"lastName":["Zanoni"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. mariagrazia.zanoni@izsler.it."]},{"fullName":["Tironi M"],"firstName":["Marco"],"lastName":["Tironi"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. marco.tironi@izsler.it."]},{"fullName":["Bertoletti M"],"firstName":["Marco"],"lastName":["Bertoletti"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. marco.bertoletti90@gmail.com."]},{"fullName":["Defilippo F"],"firstName":["Francesco"],"lastName":["Defilippo"],"initials":["F"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. francesco.defilippo@izsler.it."]},{"fullName":["Moreno A"],"firstName":["Ana"],"lastName":["Moreno"],"initials":["A"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. anamaria.morenomartin@izsler.it."]},{"fullName":["Farioli M"],"firstName":["Marco"],"lastName":["Farioli"],"initials":["M"],"affiliation":["Regional Health Authority of Lombardy, Milan 20124, Italy. Marco_farioli@regione.lombardia.it."]},{"fullName":["Piatti A"],"firstName":["Alessandra"],"lastName":["Piatti"],"initials":["A"],"affiliation":["Regional Health Authority of Lombardy, Milan 20124, Italy. Alessandra_piatti@regione.lombardia.it."]},{"fullName":["Dottori M"],"firstName":["Michele"],"lastName":["Dottori"],"initials":["M"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. michele.dottori@izsler.it."]},{"fullName":["Lelli D"],"firstName":["Davide"],"lastName":["Lelli"],"initials":["D"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. davide.lelli@izsler.it."]},{"fullName":["Lavazza A"],"firstName":["Antonio"],"lastName":["Lavazza"],"initials":["A"],"authorId":[{"_":"0000-0002-7646-3592","$":{"type":"ORCID"}}],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. antonio.lavazza@izsler.it."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7646-3592","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["13"],"journalIssueId":["2423132"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["International journal of environmental research and public health"],"ISOAbbreviation":["Int J Environ Res Public Health"],"medlineAbbreviation":["Int J Environ Res Public Health"],"NLMid":["101238455"],"ISSN":["1661-7827"],"ESSN":["1660-4601"]}]}],"pubYear":["2016"],"abstractText":["From 1 May 2015 to 31 October 2015 over 20 million visitors from all over the world visited the Universal Exhibition (EXPO) hosted by Milan (Lombardy region, Italy), raising concerns about the possible introduction of mosquito-borne diseases from endemic countries. The entomological surveillance protocol performed in Lombardy over the last three years was implemented in the EXPO area and in the two major regional airports using both Center for Disease Control CO₂ and Biogents Sentinel traps. This surveillance aimed to estimate the presence and densities of putative vectors, and also to support investigations, including the vector species involved and area of diffusion, on the local spread of Chikungunya, Dengue and West Nile viruses (WNV) by competent vectors. From 3544 mosquitoes belonging to five different species, 28 pools of Culex spp. and 45 pools of Aedes spp. were screened for the presence of WNV, and for both Chikungunya and flaviviruses, respectively. The entomological surveillance highlighted a low density of potential vectors in the surveyed areas and did not reveal the presence of Chikungunya or Dengue viruses in the local competent vectors inside the EXPO area or in the two airports. In addition, the surveillance reported a low density of Culex spp. mosquitoes, which all tested negative for WNV."],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\", Brescia 25124, Italy. mario.chiari@izsler.it."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["West Nile virus","Dengue","Chikungunya","Mosquito-borne Diseases","Entomological Surveillance","Lombardy Region","Expo 2015"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962230?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962230"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijerph13070689"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-12"],"dateOfRevision":["2016-08-02"],"electronicPublicationDate":["2016-07-08"],"firstPublicationDate":["2016-07-08"],"cprojectID":"PMC4962230"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrv_J9hGurOzZIbg","_score":1,"_source":{"id":["27460671"],"source":["MED"],"pmid":["27460671"],"pmcid":["PMC4962480"],"doi":["10.1186/s13071-016-1713-0"],"title":["Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C, in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand."],"authorString":["Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkosol P, Sukontason K, Walton C, Somboon P."],"authorList":[{"author":[{"fullName":["Plernsub S"],"firstName":["Suriya"],"lastName":["Plernsub"],"initials":["S"],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Saingamsook J"],"firstName":["Jassada"],"lastName":["Saingamsook"],"initials":["J"],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Yanola J"],"firstName":["Jintana"],"lastName":["Yanola"],"initials":["J"],"affiliation":["Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Lumjuan N"],"firstName":["Nongkran"],"lastName":["Lumjuan"],"initials":["N"],"affiliation":["Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Tippawangkosol P"],"firstName":["Pongsri"],"lastName":["Tippawangkosol"],"initials":["P"],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Sukontason K"],"firstName":["Kom"],"lastName":["Sukontason"],"initials":["K"],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."]},{"fullName":["Walton C"],"firstName":["Catherine"],"lastName":["Walton"],"initials":["C"],"affiliation":["Faculty of Life Sciences, University of Manchester, Manchester, UK."]},{"fullName":["Somboon P"],"firstName":["Pradya"],"lastName":["Somboon"],"initials":["P"],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. pradya.somboon@cmu.ac.th."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["417"],"abstractText":["Mutation in the voltage-gated sodium channel gene that results in knockdown resistance (kdr), is a major mechanism of pyrethroid resistance in several mosquito species. In Aedes aegypti, V1016G (occurring with and without S989P) and F1534C mutations are common and widely distributed throughout Asia. The G1016 allele is known to be associated with resistance to type I and II pyrethroids. The C1534 allele is primarily associated with resistance to type I pyrethroids and is known to be a recessive allele in conferring kdr.We performed crossing experiments using a P989 + G1016 homozygous mutant strain (UPK-R), a C1534 homozygous mutant strain (PMD-R) and a pyrethroid susceptible strain (PMD) to determine the insecticide susceptibility of different genotypic hybrids. Allele-specific PCR methods were used to confirm the genotypes. Metabolic resistance caused by oxidative enzymes and esterase enzymes was ruled out by the addition of piperonyl butoxide (PBO) and bis(4-nitrophenyl)-phosphate, BNPP), respectively.The median lethal concentration (LC50) of deltamethrin susceptibility of a S/P989 + V/G1016 + F/F1534 double heterozygous hybrid from the UPK-R × PMD cross was 0.57 (95 % CI: 0.51-0.63) μgl(-1), which was about 12-fold lower than for UPK-R, 6.98 (6.10-8.04) μgl(-1), and only about 4-fold greater than the susceptible PMD, 0.13 (0.12-0.15) μgl(-1). This resistance returned to 0.08 (0.07-0.09) μgl(-1) on the addition of PBO suggesting that the P989 + G1016 kdr alleles are recessive. The LC50 of the S/P989 + V/G1016 + F/C1534 triple heterozygous hybrid was 3.58 (3.21-3.95) μgl(-1), which was intermediate between that of the homozygous mutant genotypes, being 2-fold higher than the C1534 homozygote and 2-fold lower than the P989 + G1016 homozygote. These minor differences and the high LC50 values of the triple mutated heterozygote indicate there is some degree of functional equivalence of the P989 + G1016 and C1534 alleles in the heterozygote. Addition of PBO decreased the LC50 values by 2-fold, from 3.58 (3.21-3.95) to 1.52 (1.35-1.73) μgl(-1), suggesting that oxidase enzymes play a partial role in resistance. The results are consistent with the median lethal time (LT50) of the triple mutated heterozygote against 0.05 % deltamethrin paper. An adult susceptibility test also revealed that the triple mutated heterozygote was resistant to deltamethrin and permethrin.The combination of the three kdr alleles in the triple mutated heterozygote, S/P989 + V/G1016 + F/C1534, confers high resistance to pyrethroids. This heterozygous form is common in Ae. aegypti populations throughout Thailand and may have an adverse effect on the efficacy of a mosquito control program using insecticide-based approaches."],"affiliation":["Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Genetics","Aedes aegypti","KDR","Insecticide"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962480?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962480"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1713-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-27"],"dateOfRevision":["2016-07-29"],"electronicPublicationDate":["2016-07-26"],"firstPublicationDate":["2016-07-26"],"cprojectID":"PMC4962480"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwAhOUS2orL1IRy","_score":1,"_source":{"id":["27482453"],"source":["MED"],"pmid":["27482453"],"pmcid":["PMC4967965"],"title":["Human cytomegalovirus and transplantation: drug development and regulatory issues."],"authorString":["McIntosh M, Hauschild B, Miller V."],"authorList":[{"author":[{"fullName":["McIntosh M"],"firstName":["Megan"],"lastName":["McIntosh"],"initials":["M"],"affiliation":["Forum for Collaborative HIV Research , University of California Berkeley , School of Public Health , Washington DC , USA."]},{"fullName":["Hauschild B"],"firstName":["Benjamin"],"lastName":["Hauschild"],"initials":["B"],"affiliation":["Forum for Collaborative HIV Research , University of California Berkeley , School of Public Health , Washington DC , USA."]},{"fullName":["Miller V"],"firstName":["Veronica"],"lastName":["Miller"],"initials":["V"],"affiliation":["Forum for Collaborative HIV Research , University of California Berkeley , School of Public Health , Washington DC , USA."]}]}],"journalInfo":[{"issue":["3"],"volume":["2"],"journalIssueId":["2435853"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of virus eradication"],"ISOAbbreviation":["J Virus Erad"],"medlineAbbreviation":["J Virus Erad"],"NLMid":["101654142"],"ISSN":["2055-6640"],"ESSN":["2055-6659"]}]}],"pubYear":["2016"],"pageInfo":["143-148"],"abstractText":["Cytomegalovirus (CMV) infection is highly prevalent worldwide and can cause serious disease among immunocompromised individuals, including persons with HIV and transplant recipients on immunosuppressive therapies. It can also result in congenital cytomegalovirus when women are infected during pregnancy. Treatment and prevention of CMV in solid organ and haematopoietic stem cell transplant recipients is accomplished in one of three ways: (1) prophylactic therapy to prevent CMV viraemia; (2) pre-emptive therapy for those with low levels of replicating virus; and (3) treatment for established disease. Despite the high prevalence of CMV, there are few available approved drug therapies, and those that are available are hampered by toxicity and less-than-optimal efficacy. New therapies are being developed and tested; however, inconsistency in standardisation of virus levels and questions about potential endpoints in clinical trials present regulatory hurdles that must be addressed. This review covers the current state of CMV therapy, drugs currently under investigation, and clinical trial issues and questions that are in need of resolution."],"affiliation":["Forum for Collaborative HIV Research , University of California Berkeley , School of Public Health , Washington DC , USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["CMV","Cytomegalovirus","Drug Development","Regulatory Science"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967965?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967965"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-04"],"electronicPublicationDate":["2016-07-01"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4967965"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwwJ9hGurOzZIbo","_score":1,"_source":{"id":["27516628"],"source":["MED"],"pmid":["27516628"],"pmcid":["PMC4969991"],"doi":["10.2471/blt.16.000816"],"title":["In this month's Bulletin."],"journalInfo":[{"issue":["8"],"volume":["94"],"journalIssueId":["2440780"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["557"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969991?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969991"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.000816"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-12"],"dateOfRevision":["2016-08-17"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4969991"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrxQhOUS2orL1IR2","_score":1,"_source":{"id":["27516631"],"source":["MED"],"pmid":["27516631"],"pmcid":["PMC4969992"],"doi":["10.2471/blt.16.010816"],"title":["Public health round-up."],"journalInfo":[{"issue":["8"],"volume":["94"],"journalIssueId":["2440780"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["560-561"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969992?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969992"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.010816"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-12"],"dateOfRevision":["2016-08-17"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4969992"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzj_8VqgcuJwvjx","_score":1,"_source":{"id":["27096156"],"source":["MED"],"pmid":["27096156"],"pmcid":["PMC4838226"],"doi":["10.1371/journal.pntd.0004641"],"title":["A Novel Xenomonitoring Technique Using Mosquito Excreta/Feces for the Detection of Filarial Parasites and Malaria."],"authorString":["Pilotte N, Zaky WI, Abrams BP, Chadee DD, Williams SA."],"authorList":[{"author":[{"fullName":["Pilotte N"],"firstName":["Nils"],"lastName":["Pilotte"],"initials":["N"],"affiliation":["Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, Massachusetts, United States of America."]},{"fullName":["Zaky WI"],"firstName":["Weam I"],"lastName":["Zaky"],"initials":["WI"],"affiliation":["Department of Biological Sciences, Smith College, Northampton, Massachusetts, United States of America."]},{"fullName":["Abrams BP"],"firstName":["Brian P"],"lastName":["Abrams"],"initials":["BP"],"affiliation":["Department of Science, Groton School, Groton, Massachusetts, United States of America."]},{"fullName":["Chadee DD"],"firstName":["Dave D"],"lastName":["Chadee"],"initials":["DD"],"affiliation":["Department of Life Sciences, University of the West Indies, St Augustine, Trinidad and Tobago, West Indies."]},{"fullName":["Williams SA"],"firstName":["Steven A"],"lastName":["Williams"],"initials":["SA"],"affiliation":["Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, Massachusetts, United States of America."]}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004641"],"abstractText":["Given the continued successes of the world's lymphatic filariasis (LF) elimination programs and the growing successes of many malaria elimination efforts, the necessity of low cost tools and methodologies applicable to long-term disease surveillance is greater than ever before. As many countries reach the end of their LF mass drug administration programs and a growing number of countries realize unprecedented successes in their malaria intervention efforts, the need for practical molecular xenomonitoring (MX), capable of providing surveillance for disease recrudescence in settings of decreased parasite prevalence is increasingly clear. Current protocols, however, require testing of mosquitoes in pools of 25 or fewer, making high-throughput examination a challenge. The new method we present here screens the excreta/feces from hundreds of mosquitoes per pool and provides proof-of-concept for a practical alternative to traditional methodologies resulting in significant cost and labor savings.Excreta/feces of laboratory reared Aedes aegypti or Anopheles stephensi mosquitoes provided with a Brugia malayi microfilaria-positive or Plasmodium vivax-positive blood meal respectively were tested for the presence of parasite DNA using real-time PCR. A titration of samples containing various volumes of B. malayi-negative mosquito feces mixed with positive excreta/feces was also tested to determine sensitivity of detection. Real-time PCR amplification of B. malayi and P. vivax DNA from the excreta/feces of infected mosquitoes was demonstrated, and B. malayi DNA in excreta/feces from one to two mf-positive blood meal-receiving mosquitoes was detected when pooled with volumes of feces from as many as 500 uninfected mosquitoes.While the operationalizing of excreta/feces testing may require the development of new strategies for sample collection, the high-throughput nature of this new methodology has the potential to greatly reduce MX costs. This will prove particularly useful in post-transmission-interruption settings, where this inexpensive approach to long-term surveillance will help to stretch the budgets of LF and malaria elimination programs. Furthermore, as this methodology is adaptable to the detection of both single celled (P. vivax) and multicellular eukaryotic pathogens (B. malayi), exploration of its use for the detection of various other mosquito-borne diseases including viruses should be considered. Additionally, integration strategies utilizing excreta/feces testing for the simultaneous surveillance of multiple diseases should be explored."],"affiliation":["Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, Massachusetts, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Feces"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Brugia malayi"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Plasmodium vivax"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Helminth"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA, Protozoan"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Parasitology"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Entomology"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Costs and Cost Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["High-Throughput Screening Assays"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EC"],"qualifierName":["economics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Eradication"]}]}],"chemicalList":[{"chemical":[{"name":["DNA, Protozoan"],"registryNumber":["0"]},{"name":["DNA, Helminth"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4838226?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4838226"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004641"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-21"],"dateOfRevision":["2016-04-30"],"electronicPublicationDate":["2016-04-20"],"firstPublicationDate":["2016-04-20"],"cprojectID":"PMC4838226"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr0M_8VqgcuJwvj0","_score":1,"_source":{"id":["27455096"],"source":["MED"],"pmid":["27455096"],"pmcid":["PMC4959722"],"doi":["10.1371/journal.pone.0159943"],"title":["Selective Factors Associated with the Evolution of Codon Usage in Natural Populations of Arboviruses."],"authorString":["Velazquez-Salinas L, Zarate S, Eschbaumer M, Pereira Lobo F, Gladue DP, Arzt J, Novella IS, Rodriguez LL."],"authorList":[{"author":[{"fullName":["Velazquez-Salinas L"],"firstName":["Lauro"],"lastName":["Velazquez-Salinas"],"initials":["L"],"affiliation":["Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, United States of America."]},{"fullName":["Zarate S"],"firstName":["Selene"],"lastName":["Zarate"],"initials":["S"],"affiliation":["Autonomous University of Mexico City, Genomics Sciences Program, Mexico City, Mexico."]},{"fullName":["Eschbaumer M"],"firstName":["Michael"],"lastName":["Eschbaumer"],"initials":["M"],"affiliation":["Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, United States of America."]},{"fullName":["Pereira Lobo F"],"firstName":["Francisco"],"lastName":["Pereira Lobo"],"initials":["F"],"affiliation":["Laboratório Multiusuário de Bioinformática, Embrapa Informática Agropecuária, Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Campinas, Brazil."]},{"fullName":["Gladue DP"],"firstName":["Douglas P"],"lastName":["Gladue"],"initials":["DP"],"affiliation":["Foreign Animal Disease Research Unit, USDA/ARS Plum Island Animal Disease Center, Orient Point, New York, United States of America."]},{"fullName":["Arzt J"],"firstName":["Jonathan"],"lastName":["Arzt"],"initials":["J"],"affiliation":["Foreign Animal Disease Research Unit, USDA/ARS Plum Island Animal Disease Center, Orient Point, New York, United States of America."]},{"fullName":["Novella IS"],"firstName":["Isabel S"],"lastName":["Novella"],"initials":["IS"],"affiliation":["Department of Medical Microbiology and Immunology, College of Medicine and Life Sciences, The University of Toledo, Toledo, Ohio, United States of America."]},{"fullName":["Rodriguez LL"],"firstName":["Luis L"],"lastName":["Rodriguez"],"initials":["LL"],"affiliation":["Foreign Animal Disease Research Unit, USDA/ARS Plum Island Animal Disease Center, Orient Point, New York, United States of America."]}]}],"journalInfo":[{"issue":["7"],"volume":["11"],"journalIssueId":["2424094"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0159943"],"abstractText":["Arboviruses (arthropod borne viruses) have life cycles that include both vertebrate and invertebrate hosts with substantial differences in vector and host specificity between different viruses. Most arboviruses utilize RNA for their genetic material and are completely dependent on host tRNAs for their translation, suggesting that virus codon usage could be a target for selection. In the current study we analyzed the relative synonymous codon usage (RSCU) patterns of 26 arboviruses together with 25 vectors and hosts, including 8 vertebrates and 17 invertebrates. We used hierarchical cluster analysis (HCA) and principal component analysis (PCA) to identify trends in codon usage. HCA demonstrated that the RSCU of arboviruses reflects that of their natural hosts, but not that of dead-end hosts. Of the two major components identified by PCA, the first accounted for 62.1% of the total variance, and among the 59 codons analyzed in this study, the leucine codon CTG had the highest correlation with the first principal component, however isoleucine had the highest correlation during amino acid analysis. Nucleotide and dinucleotide composition were the variables that explained most of the total codon usage variance. The results suggest that the main factors driving the evolution of codon usage in arboviruses is based on the nucleotide and dinucleotide composition present in the host. Comparing codon usage of arboviruses and potential vector hosts can help identifying potential vectors for emerging arboviruses."],"affiliation":["Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4959722?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4959722"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0159943"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-26"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-07-25"],"firstPublicationDate":["2016-07-25"],"cprojectID":"PMC4959722"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqygJ9hGurOzZIYr","_score":1,"_source":{"id":["26624008"],"source":["MED"],"pmid":["26624008"],"pmcid":["PMC4666598"],"doi":["10.1371/journal.pntd.0004211"],"title":["Socio-economic and Climate Factors Associated with Dengue Fever Spatial Heterogeneity: A Worked Example in New Caledonia."],"authorString":["Teurlai M, Menkès CE, Cavarero V, Degallier N, Descloux E, Grangeon JP, Guillaumot L, Libourel T, Lucio PS, Mathieu-Daudé F, Mangeas M."],"authorList":[{"author":[{"fullName":["Teurlai M"],"firstName":["Magali"],"lastName":["Teurlai"],"initials":["M"],"affiliation":["UMR 182, LOCEAN, Institute for Research and Development (IRD), Noumea, New Caledonia."]},{"fullName":["Menkès CE"],"firstName":["Christophe Eugène"],"lastName":["Menkès"],"initials":["CE"],"affiliation":["UMR 182, LOCEAN, Institute for Research and Development (IRD), Noumea, New Caledonia."]},{"fullName":["Cavarero V"],"firstName":["Virgil"],"lastName":["Cavarero"],"initials":["V"],"affiliation":["Météo-France, Noumea, New Caledonia."]},{"fullName":["Degallier N"],"firstName":["Nicolas"],"lastName":["Degallier"],"initials":["N"],"affiliation":["UMR 182, Laboratoire d'Océanographie et du Climat, Expérimentation et Approches Numériques (LOCEAN), Institute for Research and Development (IRD), Paris, France."]},{"fullName":["Descloux E"],"firstName":["Elodie"],"lastName":["Descloux"],"initials":["E"],"affiliation":["Department of Internal Medicine and Infectious Diseases, Territorial Hospital Centre, Noumea, New Caledonia."]},{"fullName":["Grangeon JP"],"firstName":["Jean-Paul"],"lastName":["Grangeon"],"initials":["JP"],"affiliation":["Health Department, Direction of Health and Social Affairs of New Caledonia, Noumea, New Caledonia."]},{"fullName":["Guillaumot L"],"firstName":["Laurent"],"lastName":["Guillaumot"],"initials":["L"],"affiliation":["Medical Entomology, Institut Pasteur, Noumea, New Caledonia."]},{"fullName":["Libourel T"],"firstName":["Thérèse"],"lastName":["Libourel"],"initials":["T"],"affiliation":["UMR 228, ESPACE-DEV, Université de Montpellier II, IRD, Montpellier, France."]},{"fullName":["Lucio PS"],"firstName":["Paulo Sergio"],"lastName":["Lucio"],"initials":["PS"],"affiliation":["Centro de Ciências Exatas e da Terra (CCET), Universidade Federal do Rio Grande do Norte (UFRN), Campus Universitário-Lagoa Nova, Brazil."]},{"fullName":["Mathieu-Daudé F"],"firstName":["Françoise"],"lastName":["Mathieu-Daudé"],"initials":["F"],"affiliation":["UMR 224 MIVEGEC, Institute for Research and Development (IRD), Noumea, New Caledonia."]},{"fullName":["Mangeas M"],"firstName":["Morgan"],"lastName":["Mangeas"],"initials":["M"],"affiliation":["UMR 228, ESPACE-DEV, Université de Montpellier II, IRD, Montpellier, France."]}]}],"journalInfo":[{"issue":["12"],"volume":["9"],"journalIssueId":["2347466"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004211"],"abstractText":["Understanding the factors underlying the spatio-temporal distribution of infectious diseases provides useful information regarding their prevention and control. Dengue fever spatio-temporal patterns result from complex interactions between the virus, the host, and the vector. These interactions can be influenced by environmental conditions. Our objectives were to analyse dengue fever spatial distribution over New Caledonia during epidemic years, to identify some of the main underlying factors, and to predict the spatial evolution of dengue fever under changing climatic conditions, at the 2100 horizon.We used principal component analysis and support vector machines to analyse and model the influence of climate and socio-economic variables on the mean spatial distribution of 24,272 dengue cases reported from 1995 to 2012 in thirty-three communes of New Caledonia. We then modelled and estimated the future evolution of dengue incidence rates using a regional downscaling of future climate projections.The spatial distribution of dengue fever cases is highly heterogeneous. The variables most associated with this observed heterogeneity are the mean temperature, the mean number of people per premise, and the mean percentage of unemployed people, a variable highly correlated with people's way of life. Rainfall does not seem to play an important role in the spatial distribution of dengue cases during epidemics. By the end of the 21st century, if temperature increases by approximately 3 °C, mean incidence rates during epidemics could double.In New Caledonia, a subtropical insular environment, both temperature and socio-economic conditions are influencing the spatial spread of dengue fever. Extension of this study to other countries worldwide should improve the knowledge about climate influence on dengue burden and about the complex interplay between different factors. This study presents a methodology that can be used as a step by step guide to model dengue spatial heterogeneity in other countries."],"affiliation":["UMR 182, LOCEAN, Institute for Research and Development (IRD), Noumea, New Caledonia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Incidence"]},{"majorTopic_YN":["N"],"descriptorName":["Multivariate Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Environment"]},{"majorTopic_YN":["N"],"descriptorName":["Temperature"]},{"majorTopic_YN":["N"],"descriptorName":["Climate"]},{"majorTopic_YN":["N"],"descriptorName":["Rain"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Models, Biological"]},{"majorTopic_YN":["N"],"descriptorName":["Socioeconomic Factors"]},{"majorTopic_YN":["N"],"descriptorName":["New Caledonia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Climate Change"]},{"majorTopic_YN":["Y"],"descriptorName":["Epidemics"]},{"majorTopic_YN":["N"],"descriptorName":["Spatial Analysis"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4666598?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4666598"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004211"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-08"],"dateOfCreation":["2015-12-02"],"dateOfRevision":["2015-12-14"],"electronicPublicationDate":["2015-12-01"],"firstPublicationDate":["2015-12-01"],"cprojectID":"PMC4666598"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqy5_8VqgcuJwvgs","_score":1,"_source":{"id":["26084275"],"source":["MED"],"pmid":["26084275"],"pmcid":["PMC4472270"],"doi":["10.1186/s12889-015-1913-0"],"title":["Cross-sectional community-based study of the socio-demographic factors associated with the prevalence of dengue in the eastern part of Sudan in 2011."],"authorString":["Soghaier MA, Himatt S, Osman KE, Okoued SI, Seidahmed OE, Beatty ME, Elmusharaf K, Khogali J, Shingrai NH, Elmangory MM."],"authorList":[{"author":[{"fullName":["Soghaier MA"],"firstName":["Mohammed A"],"lastName":["Soghaier"],"initials":["MA"],"affiliation":["Epidemiology and Zoonotic Diseases, Federal Ministry of Health, Osman Digna Street with Nile Avenue, PO Box 303, 1111, Khartoum, Sudan. soghaier@gmail.com."]},{"fullName":["Himatt S"],"firstName":["Sayed"],"lastName":["Himatt"],"initials":["S"],"affiliation":["Present Address: Supreme Council of Health, Doha, Qatar. sayedntp@yahoo.com."]},{"fullName":["Osman KE"],"firstName":["Kamal ElDin"],"lastName":["Osman"],"initials":["KE"],"affiliation":["Tiabah University, College of Medicine, Family and Community Medicine, Riyadh, Saudi Arabia. drtkamal@hotmail.com."]},{"fullName":["Okoued SI"],"firstName":["Somia I"],"lastName":["Okoued"],"initials":["SI"],"affiliation":["Emergency and Humanitarian Action, Federal Ministry of Health, Khartoum, Sudan. somiaokoued@gmail.com."]},{"fullName":["Seidahmed OE"],"firstName":["Osama E"],"lastName":["Seidahmed"],"initials":["OE"],"affiliation":["Department of Medical Entomology, National Public Health Laboratory, Khartoum, Sudan. osamamekki@gmail.com."]},{"fullName":["Beatty ME"],"firstName":["Mark E"],"lastName":["Beatty"],"initials":["ME"],"affiliation":["Biogen Idec, Cambridge, MA, USA. mark.e.beatty@hotmail.com."]},{"fullName":["Elmusharaf K"],"firstName":["Khalifa"],"lastName":["Elmusharaf"],"initials":["K"],"authorId":[{"_":"0000-0001-8652-1867","$":{"type":"ORCID"}}],"affiliation":["Department of Epidemiology and Public Health Medicine, The Royal College of Surgeons in Ireland, Manama, Bahrain. khalifa_elmusharaf@yahoo.com."]},{"fullName":["Khogali J"],"firstName":["Jeahan"],"lastName":["Khogali"],"initials":["J"],"affiliation":["Epidemiology and Zoonotic Diseases, Kassala State Ministry of Health, Kassala, Sudan. jhbeeshi@gmail.com."]},{"fullName":["Shingrai NH"],"firstName":["Nijood H"],"lastName":["Shingrai"],"initials":["NH"],"affiliation":["Epidemiology and Zoonotic Diseases, Kassala State Ministry of Health, Kassala, Sudan. nijoodshingrai@gmail.com."]},{"fullName":["Elmangory MM"],"firstName":["Mutasim M"],"lastName":["Elmangory"],"initials":["MM"],"affiliation":["Sudan National Public Health Laboratory, Khartoum, Sudan. mangory2000@gmail.com."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-8652-1867","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2256720"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BMC public health"],"ISOAbbreviation":["BMC Public Health"],"medlineAbbreviation":["BMC Public Health"],"NLMid":["100968562"],"ESSN":["1471-2458"]}]}],"pubYear":["2015"],"pageInfo":["558"],"abstractText":["Dengue is caused by an arthropod-borne flavivirus. Infection can be either primary or secondary based on serology, with each stage of the disease characterized by specific serological conversion and antibody formation. Further study is needed to fully identify the factors associated with and predisposing to dengue infection. The objective of this study was to identify socio-demographic factors associated with the prevalence of dengue serotypes in Kassala State in the eastern part of Sudan in 2011.This was a cross-sectional community-based study with 530 participants who were randomly selected through multi-stage cluster sampling. Dengue serotype prevalence was determined using capture Enzyme-linked immunosorbent assay (ELISA). ELISA IgG. A multivariate logistic regression model was designed to measure the strength of associations between socio-demographic factors and dengue serotype prevalence. All participants who tested negative for dengue were used as the statistical reference group.From this study, the prevalence of dengue in Kassala was estimated to be 9.4% (95% CI: 7.1-12.3). Lack of knowledge about dengue fever disease (OR 2.8, 95% CI: 1.24-6.53) and a household density of more than 3 people per room (OR 2.1, 95% CI: 1.06-4.09) were the most important factors associated with dengue infection among the study population.Community-oriented interventions are needed to modify existing social behaviors to reduce the risk of dengue in the eastern part of Sudan. Additional studies are also required in this field."],"affiliation":["Epidemiology and Zoonotic Diseases, Federal Ministry of Health, Osman Digna Street with Nile Avenue, PO Box 303, 1111, Khartoum, Sudan. soghaier@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin G"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Enzyme-Linked Immunosorbent Assay"]},{"majorTopic_YN":["N"],"descriptorName":["Prevalence"]},{"majorTopic_YN":["N"],"descriptorName":["Cross-Sectional Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Family Characteristics"]},{"majorTopic_YN":["Y"],"descriptorName":["Demography"]},{"majorTopic_YN":["Y"],"descriptorName":["Social Environment"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Aged, 80 and over"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Sudan"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Community-Based Participatory Research"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["Immunoglobulin G"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4472270?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4472270"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12889-015-1913-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-12-09"],"dateOfCreation":["2015-06-18"],"dateOfRevision":["2015-06-20"],"electronicPublicationDate":["2015-06-18"],"firstPublicationDate":["2015-06-18"],"cprojectID":"PMC4472270"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq31J9hGurOzZIY1","_score":1,"_source":{"id":["26925368"],"source":["MED"],"pmid":["26925368"],"pmcid":["PMC4757633"],"title":["Wolbachia Biocontrol Strategies for Arboviral Diseases and the Potential Influence of Resident Wolbachia Strains in Mosquitoes."],"authorString":["Jeffries CL, Walker T."],"authorList":[{"author":[{"fullName":["Jeffries CL"],"firstName":["Claire L"],"lastName":["Jeffries"],"initials":["CL"],"affiliation":["Department of Disease Control, Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT UK."]},{"fullName":["Walker T"],"firstName":["Thomas"],"lastName":["Walker"],"initials":["T"],"affiliation":["Department of Disease Control, Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT UK."]}]}],"journalInfo":[{"volume":["3"],"journalIssueId":["2379523"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Current tropical medicine reports"],"ISOAbbreviation":["Curr Trop Med Rep"],"medlineAbbreviation":["Curr Trop Med Rep"],"NLMid":["101625249"],"ESSN":["2196-3045"]}]}],"pubYear":["2016"],"pageInfo":["20-25"],"abstractText":["Arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. The absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. The endosymbiotic bacterium Wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. Resident strains of Wolbachia inhibit viral replication in Drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows rapid invasion of insect populations. Transinfection of Wolbachia strains into the principle mosquito vector of dengue virus, Stegomyia aegypti, has resulted in dengue-refractory mosquito lines with minimal effects on mosquito fitness. Wolbachia strains have now been established in wild St. aegypti populations through open releases in dengue-endemic countries. In this review, we outline the current state of Wolbachia-based biocontrol strategies for dengue and discuss the potential impact of resident Wolbachia strains for additional target mosquito species that transmit arboviruses."],"affiliation":["Department of Disease Control, Faculty of Infectious Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT UK."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"grantsList":[{"grant":[{"grantId":["101285"],"agency":["Wellcome Trust"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Arboviruses","Mosquitoes","Mosquito Biocontrol","Wolbachia Bacteria"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4757633?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4757633"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-29"],"dateOfRevision":["2016-03-03"],"electronicPublicationDate":["2016-02-02"],"firstPublicationDate":["2016-02-02"],"cprojectID":"PMC4757633"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq3qhOUS2orL1IO6","_score":1,"_source":{"id":["26527283"],"source":["MED"],"pmid":["26527283"],"pmcid":["PMC4630907"],"doi":["10.1186/s12879-015-1226-z"],"title":["One-step real-time RT-PCR assays for serotyping dengue virus in clinical samples."],"authorString":["Alm E, Lindegren G, Falk KI, Lagerqvist N."],"authorList":[{"author":[{"fullName":["Alm E"],"firstName":["Erik"],"lastName":["Alm"],"initials":["E"],"affiliation":["Department of Microbiology, The Public Health Agency of Sweden, SE-171 82, Solna, Sweden. erik.alm@folkhalsomyndigheten.se."]},{"fullName":["Lindegren G"],"firstName":["Gunnel"],"lastName":["Lindegren"],"initials":["G"],"affiliation":["Department of Microbiology, The Public Health Agency of Sweden, SE-171 82, Solna, Sweden. gunnel.lindegren@folkhalsomyndigheten.se."]},{"fullName":["Falk KI"],"firstName":["Kerstin Ingrid"],"lastName":["Falk"],"initials":["KI"],"affiliation":["Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden. kerstin.falk@folkhalsomyndigheten.se."]},{"fullName":["Lagerqvist N"],"firstName":["Nina"],"lastName":["Lagerqvist"],"initials":["N"],"affiliation":["Department of Microbiology, The Public Health Agency of Sweden, SE-171 82, Solna, Sweden. nina.lagerqvist@folkhalsomyndigheten.se."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2262623"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BMC infectious diseases"],"ISOAbbreviation":["BMC Infect. Dis."],"medlineAbbreviation":["BMC Infect Dis"],"NLMid":["100968551"],"ESSN":["1471-2334"]}]}],"pubYear":["2015"],"pageInfo":["493"],"abstractText":["Dengue is one of the leading causes of morbidity in tropical and subtropical regions and infection with any of the four dengue virus serotypes (DENV1-4) result in a wide range of clinical manifestations. Given the geographic expansion of DENV1-4, assays for serotyping are needed to be able to perform surveillance and epidemiological studies. In this study, we describe the design and validation of one-step real-time serotype-specific DENV RT-PCR assays.The DENV1, DENV2, DENV3, and DENV4 RT-PCR assays were designed using all available whole genome DENV sequences in the NCBI nucleotide collection. Because of the high mutation rates of RNA viruses, the assays were performed in singleplex format to enable quick modifications to the primer and probe sequences when new genetic variants emerge. The analytical performance of the RT-PCR assays were evaluated using in vitro transcribed RNA and their specificity was determined by testing 24 DENV isolates, external DENV control panels and RNA preparation of non-DENV flaviviruses and non-dengue clinical samples. Additionally, the clinical performance of the serotype-specific DENV RT-PCR were compared to that of the CDC DENV-1-4 RT-PCR using 85 clinical samples collected from patients presenting with acute dengue.The RT-PCR assays were found to be specific for their respective serotype and did not cross-react with other flaviviruses or human mRNA. All assays had a linear dynamic range of 10(2) to 10(6) copies/reaction with detection limits between 12 and 44 copies/reaction. When testing sera from 85 confirmed acute dengue cases, the serotype-specific DENV RT-PCR assays had 100 % positive agreement with the FDA-approved CDC DENV-1-4 RT-PCR assay performed in a singleplex format. Additionally 15 samples that tested negative in the CDC DENV-1-4- RT-PCR assay were found positive using the serotype-specific DENV RT-PCR assays.Our results suggest that these RT-PCR assays are useful alternatives to existing methods for serotyping DENV in clinical sera."],"affiliation":["Department of Microbiology, The Public Health Agency of Sweden, SE-171 82, Solna, Sweden. erik.alm@folkhalsomyndigheten.se."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Serum"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["DNA Primers"]},{"majorTopic_YN":["N"],"descriptorName":["Serotyping"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cross Reactions"]},{"majorTopic_YN":["N"],"descriptorName":["Limit of Detection"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["DNA Primers"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4630907?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4630907"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12879-015-1226-z"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["refseq"," gen"," "]}],"dateOfCompletion":["2016-04-22"],"dateOfCreation":["2015-11-03"],"dateOfRevision":["2015-11-05"],"electronicPublicationDate":["2015-11-02"],"firstPublicationDate":["2015-11-02"],"cprojectID":"PMC4630907"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq4XhOUS2orL1IO_","_score":1,"_source":{"id":["26140529"],"source":["MED"],"pmid":["26140529"],"pmcid":["PMC4681859"],"doi":["10.1038/ismej.2015.83"],"title":["Ecophysiology of an uncultivated lineage of Aigarchaeota from an oxic, hot spring filamentous 'streamer' community."],"authorString":["Beam JP, Jay ZJ, Schmid MC, Rusch DB, Romine MF, Jennings Rde M, Kozubal MA, Tringe SG, Wagner M, Inskeep WP."],"authorList":[{"author":[{"fullName":["Beam JP"],"firstName":["Jacob P"],"lastName":["Beam"],"initials":["JP"],"affiliation":["Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA."]},{"fullName":["Jay ZJ"],"firstName":["Zackary J"],"lastName":["Jay"],"initials":["ZJ"],"affiliation":["Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA."]},{"fullName":["Schmid MC"],"firstName":["Markus C"],"lastName":["Schmid"],"initials":["MC"],"affiliation":["Divison of Microbial Ecology, University of Vienna, Vienna, Austria."]},{"fullName":["Rusch DB"],"firstName":["Douglas B"],"lastName":["Rusch"],"initials":["DB"],"affiliation":["Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN, USA."]},{"fullName":["Romine MF"],"firstName":["Margaret F"],"lastName":["Romine"],"initials":["MF"],"authorId":[{"_":"0000-0002-0968-7641","$":{"type":"ORCID"}}],"affiliation":["Pacific Northwest National Laboratory, Richland, WA, USA."]},{"fullName":["Jennings Rde M"],"firstName":["Ryan de M"],"lastName":["Jennings"],"initials":["Rde M"],"affiliation":["Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA."]},{"fullName":["Kozubal MA"],"firstName":["Mark A"],"lastName":["Kozubal"],"initials":["MA"],"affiliation":["Sustainable Bioproducts LLC, Bozeman, MT, USA."]},{"fullName":["Tringe SG"],"firstName":["Susannah G"],"lastName":["Tringe"],"initials":["SG"],"authorId":[{"_":"0000-0001-6479-8427","$":{"type":"ORCID"}}],"affiliation":["Department of Energy-Joint Genome Institute, Walnut Creek, CA, USA."]},{"fullName":["Wagner M"],"firstName":["Michael"],"lastName":["Wagner"],"initials":["M"],"authorId":[{"_":"0000-0002-9778-7684","$":{"type":"ORCID"}}],"affiliation":["Divison of Microbial Ecology, University of Vienna, Vienna, Austria."]},{"fullName":["Inskeep WP"],"firstName":["William P"],"lastName":["Inskeep"],"initials":["WP"],"affiliation":["Pacific Northwest National Laboratory, Richland, WA, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0968-7641","$":{"type":"ORCID"}},{"_":"0000-0001-6479-8427","$":{"type":"ORCID"}},{"_":"0000-0002-9778-7684","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["10"],"journalIssueId":["2354563"],"dateOfPublication":["2016 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["The ISME journal"],"ISOAbbreviation":["ISME J"],"medlineAbbreviation":["ISME J"],"NLMid":["101301086"],"ISSN":["1751-7362"],"ESSN":["1751-7370"]}]}],"pubYear":["2016"],"pageInfo":["210-224"],"abstractText":["The candidate archaeal phylum 'Aigarchaeota' contains microorganisms from terrestrial and subsurface geothermal ecosystems. The phylogeny and metabolic potential of Aigarchaeota has been deduced from several recent single-cell amplified genomes; however, a detailed description of their metabolic potential and in situ transcriptional activity is absent. Here, we report a comprehensive metatranscriptome-based reconstruction of the in situ metabolism of Aigarchaeota in an oxic, hot spring filamentous 'streamer' community. Fluorescence in situ hybridization showed that these newly discovered Aigarchaeota are filamentous, which is consistent with the presence and transcription of an actin-encoding gene. Aigarchaeota filaments are intricately associated with other community members, which include both bacteria (for example, filamentous Thermocrinis spp.) and archaea. Metabolic reconstruction of genomic and metatranscriptomic data suggests that this aigarchaeon is an aerobic, chemoorganoheterotroph with autotrophic potential. A heme copper oxidase complex was identified in the environmental genome assembly and highly transcribed in situ. Potential electron donors include acetate, fatty acids, amino acids, sugars and aromatic compounds, which may originate from extracellular polymeric substances produced by other microorganisms shown to exist in close proximity and/or autochthonous dissolved organic carbon (OC). Transcripts related to genes specific to each of these potential electron donors were identified, indicating that this aigarchaeon likely utilizes several OC substrates. Characterized members of this lineage cannot synthesize heme, and other cofactors and vitamins de novo, which suggests auxotrophy. We propose the name Candidatus 'Calditenuis aerorheumensis' for this aigarchaeon, which describes its filamentous morphology and its primary electron acceptor, oxygen."],"affiliation":["Thermal Biology Institute and Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"grantsList":[{"grant":[{"grantId":["294343"],"agency":["European Research Council"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Archaea"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["In Situ Hybridization, Fluorescence"]},{"majorTopic_YN":["N"],"descriptorName":["Ecosystem"]},{"majorTopic_YN":["N"],"descriptorName":["Hot Springs"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]},{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Genome, Archaeal"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Metagenomics"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4681859?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4681859"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/ismej.2015.83"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["bioproject"]}],"dateOfCompletion":["2016-08-15"],"dateOfCreation":["2015-12-15"],"dateOfRevision":["2016-07-13"],"electronicPublicationDate":["2015-07-03"],"firstPublicationDate":["2015-07-03"],"embargoDate":["2016-01-01"],"cprojectID":"PMC4681859"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq44_8VqgcuJwvg0","_score":1,"_source":{"id":["26226613"],"source":["MED"],"pmid":["26226613"],"pmcid":["PMC4520491"],"doi":["10.1371/journal.pone.0133602"],"title":["Making Mosquito Taxonomy Useful: A Stable Classification of Tribe Aedini that Balances Utility with Current Knowledge of Evolutionary Relationships."],"authorString":["Wilkerson RC, Linton YM, Fonseca DM, Schultz TR, Price DC, Strickman DA."],"authorList":[{"author":[{"fullName":["Wilkerson RC"],"firstName":["Richard C"],"lastName":["Wilkerson"],"initials":["RC"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington DC, United States of America."]},{"fullName":["Linton YM"],"firstName":["Yvonne-Marie"],"lastName":["Linton"],"initials":["YM"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington DC, United States of America; Walter Reed Biosystematics Unit, Museum Support Center, Smithsonian Institution, Suitland, Maryland, United States of America; Department of Entomology, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America; Faculty of Preventative Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America."]},{"fullName":["Fonseca DM"],"firstName":["Dina M"],"lastName":["Fonseca"],"initials":["DM"],"authorId":[{"_":"0000-0003-4726-7100","$":{"type":"ORCID"}}],"affiliation":["Entomology Department, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, United States of America."]},{"fullName":["Schultz TR"],"firstName":["Ted R"],"lastName":["Schultz"],"initials":["TR"],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington DC, United States of America."]},{"fullName":["Price DC"],"firstName":["Dana C"],"lastName":["Price"],"initials":["DC"],"affiliation":["Entomology Department, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, New Jersey, United States of America."]},{"fullName":["Strickman DA"],"firstName":["Daniel A"],"lastName":["Strickman"],"initials":["DA"],"affiliation":["Global Health Program, Bill and Melinda Gates Foundation, Seattle, Washington, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-4726-7100","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7"],"volume":["10"],"journalIssueId":["2299142"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0133602"],"abstractText":["The tribe Aedini (Family Culicidae) contains approximately one-quarter of the known species of mosquitoes, including vectors of deadly or debilitating disease agents. This tribe contains the genus Aedes, which is one of the three most familiar genera of mosquitoes. During the past decade, Aedini has been the focus of a series of extensive morphology-based phylogenetic studies published by Reinert, Harbach, and Kitching (RH&K). Those authors created 74 new, elevated or resurrected genera from what had been the single genus Aedes, almost tripling the number of genera in the entire family Culicidae. The proposed classification is based on subjective assessments of the \"number and nature of the characters that support the branches\" subtending particular monophyletic groups in the results of cladistic analyses of a large set of morphological characters of representative species. To gauge the stability of RH&K's generic groupings we reanalyzed their data with unweighted parsimony jackknife and maximum-parsimony analyses, with and without ordering 14 of the characters as in RH&K. We found that their phylogeny was largely weakly supported and their taxonomic rankings failed priority and other useful taxon-naming criteria. Consequently, we propose simplified aedine generic designations that 1) restore a classification system that is useful for the operational community; 2) enhance the ability of taxonomists to accurately place new species into genera; 3) maintain the progress toward a natural classification based on monophyletic groups of species; and 4) correct the current classification system that is subject to instability as new species are described and existing species more thoroughly defined. We do not challenge the phylogenetic hypotheses generated by the above-mentioned series of morphological studies. However, we reduce the ranks of the genera and subgenera of RH&K to subgenera or informal species groups, respectively, to preserve stability as new data become available."],"affiliation":["Department of Entomology, National Museum of Natural History, Smithsonian Institution, Washington DC, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Population Groups"]},{"majorTopic_YN":["N"],"descriptorName":["Biological Evolution"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4520491?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4520491"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0133602"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["10"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-04"],"dateOfCreation":["2015-08-03"],"dateOfRevision":["2015-08-07"],"electronicPublicationDate":["2015-07-30"],"firstPublicationDate":["2015-07-30"],"cprojectID":"PMC4520491"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq5XJ9hGurOzZIY4","_score":1,"_source":{"id":["26914330"],"source":["MED"],"pmid":["26914330"],"pmcid":["PMC4767410"],"doi":["10.1371/journal.pntd.0004517"],"title":["Zika Virus Infection and Stillbirths: A Case of Hydrops Fetalis, Hydranencephaly and Fetal Demise."],"authorString":["Sarno M, Sacramento GA, Khouri R, do Rosário MS, Costa F, Archanjo G, Santos LA, Nery N, Vasilakis N, Ko AI, de Almeida AR."],"authorList":[{"author":[{"fullName":["Sarno M"],"firstName":["Manoel"],"lastName":["Sarno"],"initials":["M"],"affiliation":["Faculdade de Medicina da Bahia and Instituto da Saúde Coletiva, Universidade Federal da Bahia, Salvador, Bahia, Brazil."]},{"fullName":["Sacramento GA"],"firstName":["Gielson A"],"lastName":["Sacramento"],"initials":["GA"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Sáude, Salvador, Bahia, Brazil."]},{"fullName":["Khouri R"],"firstName":["Ricardo"],"lastName":["Khouri"],"initials":["R"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Sáude, Salvador, Bahia, Brazil."]},{"fullName":["do Rosário MS"],"firstName":["Mateus S"],"lastName":["do Rosário"],"initials":["MS"],"affiliation":["Hospital Geral Roberto Santos, Secretaria Estadual da Saúde da Bahia, Salvador, Bahia, Brazil."]},{"fullName":["Costa F"],"firstName":["Federico"],"lastName":["Costa"],"initials":["F"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America."]},{"fullName":["Archanjo G"],"firstName":["Gracinda"],"lastName":["Archanjo"],"initials":["G"],"affiliation":["Hospital Geral Roberto Santos, Secretaria Estadual da Saúde da Bahia, Salvador, Bahia, Brazil."]},{"fullName":["Santos LA"],"firstName":["Luciane A"],"lastName":["Santos"],"initials":["LA"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Sáude, Salvador, Bahia, Brazil."]},{"fullName":["Nery N Jr"],"firstName":["Nivison"],"lastName":["Nery"],"initials":["N"],"affiliation":["Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Sáude, Salvador, Bahia, Brazil."]},{"fullName":["Vasilakis N"],"firstName":["Nikos"],"lastName":["Vasilakis"],"initials":["N"],"affiliation":["Department of Pathology and Center of Biodefense and Emerging Infectious Diseases, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, Texas, United States of America."]},{"fullName":["Ko AI"],"firstName":["Albert I"],"lastName":["Ko"],"initials":["AI"],"affiliation":["Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America."]},{"fullName":["de Almeida AR"],"firstName":["Antonio R P"],"lastName":["de Almeida"],"initials":["AR"],"affiliation":["Faculdade de Medicina da Bahia and Instituto da Saúde Coletiva, Universidade Federal da Bahia, Salvador, Bahia, Brazil."]}]}],"journalInfo":[{"issue":["2"],"volume":["10"],"journalIssueId":["2368699"],"dateOfPublication":["2016 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2016"],"printPublicationDate":["2016-02-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004517"],"abstractText":["The rapid spread of Zika virus in the Americas and current outbreak of microcephaly in Brazil has raised attention to the possible deleterious effects that the virus may have on fetuses.We report a case of a 20-year-old pregnant woman who was referred to our service after a large Zika virus outbreak in the city of Salvador, Brazil with an ultrasound examination that showed intrauterine growth retardation of the fetus at the 18th gestational week. Ultrasound examinations in the 2nd and 3rd trimesters demonstrated severe microcephaly, hydranencephaly, intracranial calcifications and destructive lesions of posterior fossa, in addition to hydrothorax, ascites and subcutaneous edema. An induced labor was performed at the 32nd gestational week due to fetal demise and delivered a female fetus. ZIKV-specific real-time polymerase chain reaction amplification products were obtained from extracts of cerebral cortex, medulla oblongata and cerebrospinal and amniotic fluid, while extracts of heart, lung, liver, vitreous body of the eye and placenta did not yield detectable products.This case report provides evidence that in addition to microcephaly, there may be a link between Zika virus infection and hydrops fetalis and fetal demise. Given the recent spread of the virus, systematic investigation of spontaneous abortions and stillbirths may be warranted to evaluate the risk that ZIKV infection imparts on these outcomes."],"affiliation":["Faculdade de Medicina da Bahia and Instituto da Saúde Coletiva, Universidade Federal da Bahia, Salvador, Bahia, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Case Reports"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Hydranencephaly"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MO"],"qualifierName":["mortality"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Hydrops Fetalis"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MO"],"qualifierName":["mortality"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Pregnancy"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Stillbirth"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MO"],"qualifierName":["mortality"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4767410?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4767410"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004517"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-20"],"dateOfCreation":["2016-02-26"],"dateOfRevision":["2016-03-10"],"electronicPublicationDate":["2016-02-25"],"firstPublicationDate":["2016-02-25"],"cprojectID":"PMC4767410"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq8ZJ9hGurOzZIY_","_score":1,"_source":{"id":["26955231"],"source":["MED"],"pmid":["26955231"],"pmcid":["PMC4779854"],"doi":["10.3346/jkms.2016.31.3.331"],"title":["Zika virus Infection: New Threat in Global Health."],"authorString":["Lee J."],"authorList":[{"author":[{"fullName":["Lee J"],"firstName":["Jacob"],"lastName":["Lee"],"initials":["J"],"affiliation":["Division of Infectious Disease, College of Medicine, Hallym University, Seoul, Korea ."]}]}],"journalInfo":[{"issue":["3"],"volume":["31"],"journalIssueId":["2383969"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Journal of Korean medical science"],"ISOAbbreviation":["J. Korean Med. Sci."],"medlineAbbreviation":["J Korean Med Sci"],"NLMid":["8703518"],"ISSN":["1011-8934"],"ESSN":["1598-6357"]}]}],"pubYear":["2016"],"pageInfo":["331-332"],"affiliation":["Division of Infectious Disease, College of Medicine, Hallym University, Seoul, Korea ."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4779854?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4779854"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3346/jkms.2016.31.3.331"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-09"],"dateOfRevision":["2016-03-11"],"electronicPublicationDate":["2016-02-11"],"firstPublicationDate":["2016-02-11"],"cprojectID":"PMC4779854"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9h_8VqgcuJwvg_","_score":1,"_source":{"id":["26588076"],"source":["MED"],"pmid":["26588076"],"pmcid":["PMC4654492"],"doi":["10.1371/journal.pntd.0004226"],"title":["Deltamethrin Resistance Mechanisms in Aedes aegypti Populations from Three French Overseas Territories Worldwide."],"authorString":["Dusfour I, Zorrilla P, Guidez A, Issaly J, Girod R, Guillaumot L, Robello C, Strode C."],"authorList":[{"author":[{"fullName":["Dusfour I"],"firstName":["Isabelle"],"lastName":["Dusfour"],"initials":["I"],"affiliation":["Institut Pasteur de la Guyane, Unité d'Entomologie Médicale, Cayenne, French Guiana, France."]},{"fullName":["Zorrilla P"],"firstName":["Pilar"],"lastName":["Zorrilla"],"initials":["P"],"affiliation":["Unidad de Biología Molecular, Institut Pasteur de Montevideo and Dept. of Biochemistry, School of Medicina, Montevideo, Uruguay."]},{"fullName":["Guidez A"],"firstName":["Amandine"],"lastName":["Guidez"],"initials":["A"],"affiliation":["Institut Pasteur de la Guyane, Unité d'Entomologie Médicale, Cayenne, French Guiana, France."]},{"fullName":["Issaly J"],"firstName":["Jean"],"lastName":["Issaly"],"initials":["J"],"affiliation":["Institut Pasteur de la Guyane, Unité d'Entomologie Médicale, Cayenne, French Guiana, France."]},{"fullName":["Girod R"],"firstName":["Romain"],"lastName":["Girod"],"initials":["R"],"affiliation":["Institut Pasteur de la Guyane, Unité d'Entomologie Médicale, Cayenne, French Guiana, France."]},{"fullName":["Guillaumot L"],"firstName":["Laurent"],"lastName":["Guillaumot"],"initials":["L"],"affiliation":["Institut Pasteur de Nouvelle Calédonie, Unité de Recherche et d'Expertise en Entomologie Médicale, Noumea, New Caledonia."]},{"fullName":["Robello C"],"firstName":["Carlos"],"lastName":["Robello"],"initials":["C"],"affiliation":["Unidad de Biología Molecular, Institut Pasteur de Montevideo and Dept. of Biochemistry, School of Medicina, Montevideo, Uruguay."]},{"fullName":["Strode C"],"firstName":["Clare"],"lastName":["Strode"],"initials":["C"],"authorId":[{"_":"0000-0003-2833-8073","$":{"type":"ORCID"}}],"affiliation":["Biology Department, Edge Hill University, Ormskirk, Lancashire, United Kingdom."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-2833-8073","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["11"],"volume":["9"],"journalIssueId":["2338334"],"dateOfPublication":["2015 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2015"],"printPublicationDate":["2015-11-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004226"],"abstractText":["Aedes aegypti is a cosmopolite mosquito, vector of arboviruses. The worldwide studies of its insecticide resistance have demonstrated a strong loss of susceptibility to pyrethroids, the major class of insecticide used for vector control. French overseas territories such as French Guiana (South America), Guadeloupe islands (Lesser Antilles) as well as New Caledonia (Pacific Ocean), have encountered such resistance.We initiated a research program on the pyrethroid resistance in French Guiana, Guadeloupe and New Caledonia. Aedes aegypti populations were tested for their deltamethrin resistance level then screened by an improved microarray developed to specifically study metabolic resistance mechanisms. Cytochrome P450 genes were implicated in conferring resistance. CYP6BB2, CYP6M11, CYP6N12, CYP9J9, CYP9J10 and CCE3 genes were upregulated in the resistant populations and were common to other populations at a regional scale. The implication of these genes in resistance phenomenon is therefore strongly suggested. Other genes from detoxification pathways were also differentially regulated. Screening for target site mutations on the voltage-gated sodium channel gene demonstrated the presence of I1016 and C1534.This study highlighted the presence of a common set of differentially up-regulated detoxifying genes, mainly cytochrome P450 genes in all three populations. GUA and GUY populations shared a higher number of those genes compared to CAL. Two kdr mutations well known to be associated to pyrethroid resistance were also detected in those two populations but not in CAL. Different selective pressures and genetic backgrounds can explain such differences. These results are also compared with those obtained from other parts of the world and are discussed in the context of integrative research on vector competence."],"affiliation":["Institut Pasteur de la Guyane, Unité d'Entomologie Médicale, Cayenne, French Guiana, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Pyrethrins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Nitriles"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cytochrome P-450 Enzyme System"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insecticides"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Microarray Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Gene Expression Profiling"]},{"majorTopic_YN":["Y"],"descriptorName":["Insecticide Resistance"]},{"majorTopic_YN":["N"],"descriptorName":["Guadeloupe"]},{"majorTopic_YN":["N"],"descriptorName":["French Guiana"]},{"majorTopic_YN":["N"],"descriptorName":["New Caledonia"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Mutant Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Voltage-Gated Sodium Channels"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Nitriles"],"registryNumber":["0"]},{"name":["Insecticides"],"registryNumber":["0"]},{"name":["Cytochrome P-450 Enzyme System"],"registryNumber":["9035-51-2"]},{"name":["Pyrethrins"],"registryNumber":["0"]},{"name":["Mutant Proteins"],"registryNumber":["0"]},{"name":["decamethrin"],"registryNumber":["2JTS8R821G"]},{"name":["Voltage-Gated Sodium Channels"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4654492?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4654492"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004226"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-04"],"dateOfCreation":["2015-11-21"],"dateOfRevision":["2015-11-26"],"electronicPublicationDate":["2015-11-20"],"firstPublicationDate":["2015-11-20"],"cprojectID":"PMC4654492"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-JhOUS2orL1IPR","_score":1,"_source":{"id":["26938868"],"source":["MED"],"pmid":["26938868"],"pmcid":["PMC4777396"],"doi":["10.1371/journal.pntd.0004543"],"title":["Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus."],"authorString":["Chouin-Carneiro T, Vega-Rua A, Vazeille M, Yebakima A, Girod R, Goindin D, Dupont-Rouzeyrol M, Lourenço-de-Oliveira R, Failloux AB."],"authorList":[{"author":[{"fullName":["Chouin-Carneiro T"],"firstName":["Thais"],"lastName":["Chouin-Carneiro"],"initials":["T"],"affiliation":["Instituto Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Vega-Rua A"],"firstName":["Anubis"],"lastName":["Vega-Rua"],"initials":["A"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Paris, France."]},{"fullName":["Vazeille M"],"firstName":["Marie"],"lastName":["Vazeille"],"initials":["M"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Paris, France."]},{"fullName":["Yebakima A"],"firstName":["André"],"lastName":["Yebakima"],"initials":["A"],"affiliation":["Centre de Démoustication/Collectivité Territoriale de La Martinique, Fort-de-France, Martinique."]},{"fullName":["Girod R"],"firstName":["Romain"],"lastName":["Girod"],"initials":["R"],"affiliation":["Institut Pasteur of French Guiana, Unit of Medical Entomology, Cayenne, French Guiana."]},{"fullName":["Goindin D"],"firstName":["Daniella"],"lastName":["Goindin"],"initials":["D"],"affiliation":["Institut Pasteur of Guadeloupe, Laboratory of Medical Entomology, Environment and Health, Les Abymes, Guadeloupe."]},{"fullName":["Dupont-Rouzeyrol M"],"firstName":["Myrielle"],"lastName":["Dupont-Rouzeyrol"],"initials":["M"],"affiliation":["Institut Pasteur of New Caledonia, Dengue and Arboviruses Research and Expertise Unit, Nouméa, New Caledonia."]},{"fullName":["Lourenço-de-Oliveira R"],"firstName":["Ricardo"],"lastName":["Lourenço-de-Oliveira"],"initials":["R"],"affiliation":["Instituto Oswaldo Cruz, Rio de Janeiro, Brazil."]},{"fullName":["Failloux AB"],"firstName":["Anna-Bella"],"lastName":["Failloux"],"initials":["AB"],"affiliation":["Institut Pasteur, Arboviruses and Insect Vectors, Paris, France."]}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004543"],"abstractText":["Since the major outbreak in 2007 in the Yap Island, Zika virus (ZIKV) causing dengue-like syndromes has affected multiple islands of the South Pacific region. In May 2015, the virus was detected in Brazil and then spread through South and Central America. In December 2015, ZIKV was detected in French Guiana and Martinique. The aim of the study was to evaluate the vector competence of the mosquito spp. Aedes aegypti and Aedes albopictus from the Caribbean (Martinique, Guadeloupe), North America (southern United States), South America (Brazil, French Guiana) for the currently circulating Asian genotype of ZIKV isolated from a patient in April 2014 in New Caledonia.Mosquitoes were orally exposed to an Asian genotype of ZIKV (NC-2014-5132). Upon exposure, engorged mosquitoes were maintained at 28° ± 1 °C, a 16h:8h light:dark cycle and 80% humidity. 25-30 mosquitoes were processed at 4, 7 and 14 days post-infection (dpi). Mosquito bodies (thorax and abdomen), heads and saliva were analyzed to measure infection, dissemination and transmission, respectively. High infection but lower disseminated infection and transmission rates were observed for both Ae. aegypti and Ae. albopictus. Ae. aegypti populations from Guadeloupe and French Guiana exhibited a higher dissemination of ZIKV than the other Ae. aegypti populations examined. Transmission of ZIKV was observed in both mosquito species at 14 dpi but at a low level.This study suggests that although susceptible to infection, Ae. aegypti and Ae. albopictus were unexpectedly low competent vectors for ZIKV. This may suggest that other factors such as the large naïve population for ZIKV and the high densities of human-biting mosquitoes contribute to the rapid spread of ZIKV during the current outbreak."],"affiliation":["Instituto Oswaldo Cruz, Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Saliva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animal Structures"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humidity"]},{"majorTopic_YN":["N"],"descriptorName":["Temperature"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Americas"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777396?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777396"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004543"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-03-04"],"dateOfRevision":["2016-03-11"],"electronicPublicationDate":["2016-03-03"],"firstPublicationDate":["2016-03-03"],"cprojectID":"PMC4777396"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr0xhOUS2orL1IR_","_score":1,"_source":{"id":["27283140"],"source":["MED"],"pmid":["27283140"],"pmcid":["PMC4974447"],"doi":["10.4178/epih.e2016023"],"title":["Zika: what we do and do not know based on the experiences of Brazil."],"authorString":["Possas C."],"authorList":[{"author":[{"fullName":["Possas C"],"firstName":["Cristina"],"lastName":["Possas"],"initials":["C"],"authorId":[{"_":"0000-0002-2886-2812","$":{"type":"ORCID"}}],"affiliation":["Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-2886-2812","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["38"],"journalIssueId":["2430403"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Epidemiology and health"],"ISOAbbreviation":["Epidemiol Health"],"medlineAbbreviation":["Epidemiol Health"],"NLMid":["101519472"],"ESSN":["2092-7193"]}]}],"pubYear":["2016"],"pageInfo":["e2016023"],"abstractText":["Zika virus, which was first discovered in 1947, has become a global threat to human health as it is rapidly spreading through Latin America, the Caribbean, the US and Asia, after causing a large outbreak in the Northeast region of Brazil in 2015. There is ample evidence to support that Zika virus is associated with neurological complications such as microcephaly. The review aims to provide an overview on the complex issues involved in the emergence of Zika virus's neurological disorders and to discuss possible explanations of Zika virus introduction and dissemination in Brazil. We also suggest national and global strategies to adequately respond to the Zika virus emergence.We provide an analytical evaluation of the main issues related to the Zika outbreak in Brazil, based on available scientific literature, including government documents, and on epidemiological information from national surveillance databases.The studies on the clinical manifestations of the Zika virus infection coupled with the epidemiological surveillance information in Brazil have provided significant evidence that the Zika virus is associated with neurological disorders such as microcephaly and Guillain-Barré syndrome. Based on phylogenetic and molecular analysis, the hypothesis regarding the introduction of Zika virus in the country is that it took place following international events in 2013 and 2014, when many foreign visitors could have brought Zika virus into Brazil. The immunologically naïve status of populations in the Americas, previous infection with dengue virus, and the increased activity of Aedes aegypti might be the contributing factors for such an outbreak in Brazil. The Zika virus emergence emphasized the importance of cross-disciplinary perspective. Besides the scientific-based vector control strategies, it is important to understand the nature of the evolutionary processes involved in the viral evolution in complex ecosystems and to have social and anthropological knowledge on the conditions related to the spread of the disease in order to properly respond to the spread of the Zika virus.The experiences of Brazil have demonstrated the significance of multi-disciplinary approach in response to new and resurgent arboviral diseases and provided important lessons that could be applied to other developing countries."],"affiliation":["Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Immunity","Pathogenesis","epidemiology","Microcephaly","Guillain-barré Syndrome","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974447?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974447"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4178/epih.e2016023"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-05"],"dateOfRevision":["2016-08-11"],"electronicPublicationDate":["2016-05-31"],"firstPublicationDate":["2016-05-31"],"cprojectID":"PMC4974447"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr12_8VqgcuJwvj4","_score":1,"_source":{"id":["27384574"],"source":["MED"],"pmid":["27384574"],"pmcid":["PMC4962217"],"doi":["10.3390/ijerph13070676"],"title":["Integrating Health Research into Disaster Response: The New NIH Disaster Research Response Program."],"authorString":["Miller A, Yeskey K, Garantziotis S, Arnesen S, Bennett A, O'Fallon L, Thompson C, Reinlib L, Masten S, Remington J, Love C, Ramsey S, Rosselli R, Galluzzo B, Lee J, Kwok R, Hughes J."],"authorList":[{"author":[{"fullName":["Miller A"],"firstName":["Aubrey"],"lastName":["Miller"],"initials":["A"],"affiliation":["National Institute of Environmental Health Sciences, Bethesda, MD 20892, USA. miller.aubrey@nih.gov."]},{"fullName":["Yeskey K"],"firstName":["Kevin"],"lastName":["Yeskey"],"initials":["K"],"affiliation":["MDB, Inc., Washington, DC 20036, USA. kyeskey@michaeldbaker.com."]},{"fullName":["Garantziotis S"],"firstName":["Stavros"],"lastName":["Garantziotis"],"initials":["S"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. garantziotis@niehs.nih.gov."]},{"fullName":["Arnesen S"],"firstName":["Stacey"],"lastName":["Arnesen"],"initials":["S"],"affiliation":["National Library of Medicine, Bethesda, MD 20892, USA. arneses@mail.nlm.nih.gov."]},{"fullName":["Bennett A"],"firstName":["April"],"lastName":["Bennett"],"initials":["A"],"affiliation":["Contractor, National Institute of Environmental Health Sciences, Bethesda, MD 20892, USA. april.bennett@nih.gov."]},{"fullName":["O'Fallon L"],"firstName":["Liam"],"lastName":["O'Fallon"],"initials":["L"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. ofallon@niehs.nih.gov."]},{"fullName":["Thompson C"],"firstName":["Claudia"],"lastName":["Thompson"],"initials":["C"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. thompso1@niehs.nih.gov."]},{"fullName":["Reinlib L"],"firstName":["Les"],"lastName":["Reinlib"],"initials":["L"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. reinlib@niehs.nih.gov."]},{"fullName":["Masten S"],"firstName":["Scott"],"lastName":["Masten"],"initials":["S"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. masten@niehs.nih.gov."]},{"fullName":["Remington J"],"firstName":["James"],"lastName":["Remington"],"initials":["J"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. remingtonj@niehs.nih.gov."]},{"fullName":["Love C"],"firstName":["Cindy"],"lastName":["Love"],"initials":["C"],"affiliation":["National Library of Medicine, Bethesda, MD 20892, USA. lovec@mail.nlm.nih.gov."]},{"fullName":["Ramsey S"],"firstName":["Steve"],"lastName":["Ramsey"],"initials":["S"],"affiliation":["Social & Scientific Systems, Inc., Durham, NC 27703, USA. SRamsey@s-3.com."]},{"fullName":["Rosselli R"],"firstName":["Richard"],"lastName":["Rosselli"],"initials":["R"],"affiliation":["Social & Scientific Systems, Inc., Durham, NC 27703, USA. RRosselli@s-3.com."]},{"fullName":["Galluzzo B"],"firstName":["Betsy"],"lastName":["Galluzzo"],"initials":["B"],"affiliation":["MDB, Inc., Washington, DC 20036, USA. beagin@michaeldbaker.com."]},{"fullName":["Lee J"],"firstName":["Joy"],"lastName":["Lee"],"initials":["J"],"affiliation":["MDB, Inc., Washington, DC 20036, USA. jlee@michaeldbaker.com."]},{"fullName":["Kwok R"],"firstName":["Richard"],"lastName":["Kwok"],"initials":["R"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. Richard.Kwok@nih.gov."]},{"fullName":["Hughes J"],"firstName":["Joseph"],"lastName":["Hughes"],"initials":["J"],"affiliation":["National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. hughes3@niehs.nih.gov."]}]}],"journalInfo":[{"issue":["7"],"volume":["13"],"journalIssueId":["2423132"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["International journal of environmental research and public health"],"ISOAbbreviation":["Int J Environ Res Public Health"],"medlineAbbreviation":["Int J Environ Res Public Health"],"NLMid":["101238455"],"ISSN":["1661-7827"],"ESSN":["1660-4601"]}]}],"pubYear":["2016"],"abstractText":["The need for high quality and timely disaster research has been a topic of great discussion over the past several years. Recent high profile incidents have exposed gaps in knowledge about the health impacts of disasters or the benefits of specific interventions-such was the case with the 2010 Gulf Oil Spill and recent events associated with lead-contaminated drinking water in Flint, Michigan, and the evolving health crisis related to Zika virus disease. Our inability to perform timely research to inform the community about health and safety risks or address specific concerns further heightens anxiety and distrust. Since nearly all disasters, whether natural or man-made, have an environmental health component, it is critical that specialized research tools and trained researchers be readily available to evaluate complex exposures and health effects, especially for vulnerable sub-populations such as the elderly, children, pregnant women, and those with socioeconomic and environmental disparities. In response, the National Institute of Environmental Health Science has initiated a Disaster Research Response Program to create new tools, protocols, networks of researchers, training exercises, and outreach involving diverse groups of stakeholders to help overcome the challenges of disaster research and to improve our ability to collect vital information to reduce the adverse health impacts and improve future preparedness."],"affiliation":["National Institute of Environmental Health Sciences, Bethesda, MD 20892, USA. miller.aubrey@nih.gov."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Disasters","Environmental Health","Disaster Epidemiology","Disaster Research","Disaster Risk Reduction","Science Preparedness","Strategic Science"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962217?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4962217"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijerph13070676"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-07"],"dateOfRevision":["2016-08-02"],"electronicPublicationDate":["2016-07-04"],"firstPublicationDate":["2016-07-04"],"cprojectID":"PMC4962217"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr2BhOUS2orL1ISB","_score":1,"_source":{"id":["27494405"],"source":["MED"],"pmid":["27494405"],"pmcid":["PMC4975412"],"doi":["10.1371/journal.pntd.0004915"],"title":["Estimating Geographical Variation in the Risk of Zoonotic Plasmodium knowlesi Infection in Countries Eliminating Malaria."],"authorString":["Shearer FM, Huang Z, Weiss DJ, Wiebe A, Gibson HS, Battle KE, Pigott DM, Brady OJ, Putaporntip C, Jongwutiwes S, Lau YL, Manske M, Amato R, Elyazar IR, Vythilingam I, Bhatt S, Gething PW, Singh B, Golding N, Hay SI, Moyes CL."],"authorList":[{"author":[{"fullName":["Shearer FM"],"firstName":["Freya M"],"lastName":["Shearer"],"initials":["FM"],"affiliation":["Spatial Ecology & Epidemiology Group, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Huang Z"],"firstName":["Zhi"],"lastName":["Huang"],"initials":["Z"],"affiliation":["Malaria Atlas Project, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Weiss DJ"],"firstName":["Daniel J"],"lastName":["Weiss"],"initials":["DJ"],"affiliation":["Malaria Atlas Project, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Wiebe A"],"firstName":["Antoinette"],"lastName":["Wiebe"],"initials":["A"],"affiliation":["Spatial Ecology & Epidemiology Group, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Gibson HS"],"firstName":["Harry S"],"lastName":["Gibson"],"initials":["HS"],"affiliation":["Malaria Atlas Project, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Battle KE"],"firstName":["Katherine E"],"lastName":["Battle"],"initials":["KE"],"affiliation":["Malaria Atlas Project, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Pigott DM"],"firstName":["David M"],"lastName":["Pigott"],"initials":["DM"],"affiliation":["Institute of Health Metrics and Evaluation, University of Washington, Seattle, Washington, United States of America."]},{"fullName":["Brady OJ"],"firstName":["Oliver J"],"lastName":["Brady"],"initials":["OJ"],"affiliation":["Spatial Ecology & Epidemiology Group, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Putaporntip C"],"firstName":["Chaturong"],"lastName":["Putaporntip"],"initials":["C"],"affiliation":["Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand."]},{"fullName":["Jongwutiwes S"],"firstName":["Somchai"],"lastName":["Jongwutiwes"],"initials":["S"],"affiliation":["Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand."]},{"fullName":["Lau YL"],"firstName":["Yee Ling"],"lastName":["Lau"],"initials":["YL"],"affiliation":["Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia."]},{"fullName":["Manske M"],"firstName":["Magnus"],"lastName":["Manske"],"initials":["M"],"affiliation":["Medical Research Council (MRC) Centre for Genomics and Global Health, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Amato R"],"firstName":["Roberto"],"lastName":["Amato"],"initials":["R"],"affiliation":["Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Elyazar IR"],"firstName":["Iqbal R F"],"lastName":["Elyazar"],"initials":["IR"],"affiliation":["Eijkman-Oxford Clinical Research Unit, Jakarta, Indonesia."]},{"fullName":["Vythilingam I"],"firstName":["Indra"],"lastName":["Vythilingam"],"initials":["I"],"affiliation":["Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia."]},{"fullName":["Bhatt S"],"firstName":["Samir"],"lastName":["Bhatt"],"initials":["S"],"affiliation":["Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom."]},{"fullName":["Gething PW"],"firstName":["Peter W"],"lastName":["Gething"],"initials":["PW"],"affiliation":["Malaria Atlas Project, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Singh B"],"firstName":["Balbir"],"lastName":["Singh"],"initials":["B"],"affiliation":["Malaria Research Centre, Universiti Malaysia Sarawak, Kuching, Sarawak, Malaysia."]},{"fullName":["Golding N"],"firstName":["Nick"],"lastName":["Golding"],"initials":["N"],"affiliation":["Department of BioSciences, University of Melbourne, Parkville, Victoria, Australia."]},{"fullName":["Hay SI"],"firstName":["Simon I"],"lastName":["Hay"],"initials":["SI"],"authorId":[{"_":"0000-0002-0611-7272","$":{"type":"ORCID"}}],"affiliation":["Institute of Health Metrics and Evaluation, University of Washington, Seattle, Washington, United States of America."]},{"fullName":["Moyes CL"],"firstName":["Catherine L"],"lastName":["Moyes"],"initials":["CL"],"authorId":[{"_":"0000-0002-8028-4079","$":{"type":"ORCID"}}],"affiliation":["Spatial Ecology & Epidemiology Group, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0611-7272","$":{"type":"ORCID"}},{"_":"0000-0002-8028-4079","$":{"type":"ORCID"}},{"_":"0000-0002-8028-4079","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004915"],"abstractText":["Infection by the simian malaria parasite, Plasmodium knowlesi, can lead to severe and fatal disease in humans, and is the most common cause of malaria in parts of Malaysia. Despite being a serious public health concern, the geographical distribution of P. knowlesi malaria risk is poorly understood because the parasite is often misidentified as one of the human malarias. Human cases have been confirmed in at least nine Southeast Asian countries, many of which are making progress towards eliminating the human malarias. Understanding the geographical distribution of P. knowlesi is important for identifying areas where malaria transmission will continue after the human malarias have been eliminated.A total of 439 records of P. knowlesi infections in humans, macaque reservoir and vector species were collated. To predict spatial variation in disease risk, a model was fitted using records from countries where the infection data coverage is high. Predictions were then made throughout Southeast Asia, including regions where infection data are sparse. The resulting map predicts areas of high risk for P. knowlesi infection in a number of countries that are forecast to be malaria-free by 2025 (Malaysia, Cambodia, Thailand and Vietnam) as well as countries projected to be eliminating malaria (Myanmar, Laos, Indonesia and the Philippines).We have produced the first map of P. knowlesi malaria risk, at a fine-scale resolution, to identify priority areas for surveillance based on regions with sparse data and high estimated risk. Our map provides an initial evidence base to better understand the spatial distribution of this disease and its potential wider contribution to malaria incidence. Considering malaria elimination goals, areas for prioritised surveillance are identified."],"affiliation":["Spatial Ecology & Epidemiology Group, Oxford Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4975412?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4975412"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004915"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-06"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-05"],"firstPublicationDate":["2016-08-05"],"cprojectID":"PMC4975412"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr2cJ9hGurOzZIby","_score":1,"_source":{"id":["27489331"],"source":["MED"],"pmid":["27489331"],"pmcid":["PMC4973206"],"doi":["10.4269/ajtmh.16-0563"],"title":["Racing Against Time Against Zika."],"authorString":["Panosian Dunavan C."],"authorList":[{"author":[{"fullName":["Panosian Dunavan C"],"firstName":["Claire"],"lastName":["Panosian Dunavan"],"initials":["C"],"affiliation":["University of California, Los Angeles, CA. E-mail: cpanosian@mednet.ucla.edu."]}]}],"journalInfo":[{"issue":["2"],"volume":["95"],"journalIssueId":["2437453"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2016"],"pageInfo":["490-492"],"affiliation":["University of California, Los Angeles, CA. E-mail: cpanosian@mednet.ucla.edu."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973206?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973206"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.16-0563"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-08-24"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4973206"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr6zJ9hGurOzZIcG","_score":1,"_source":{"id":["27525286"],"source":["MED"],"pmid":["27525286"],"pmcid":["PMC4982465"],"title":["Neuropathology of Zika Virus Infection."],"authorString":["Solomon IH, Milner DA, Folkerth RD."],"authorList":[{"author":[{"fullName":["Solomon IH"],"firstName":["Isaac H"],"lastName":["Solomon"],"initials":["IH"],"affiliation":["Department of Pathology, Brigham and Women's Hospital, Boston, USA."]},{"fullName":["Milner DA"],"firstName":["Danny A"],"lastName":["Milner"],"initials":["DA"],"affiliation":["Department of Pathology, Brigham and Women's Hospital, Boston, USA."]},{"fullName":["Folkerth RD"],"firstName":["Rebecca D"],"lastName":["Folkerth"],"initials":["RD"],"affiliation":["Department of Pathology, Brigham and Women's Hospital, Boston, USA."]}]}],"journalInfo":[{"issue":["2"],"volume":["7"],"journalIssueId":["2435480"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Journal of neuroinfectious diseases"],"ISOAbbreviation":["J Neuroinfect Dis"],"medlineAbbreviation":["J Neuroinfect Dis"],"NLMid":["101609701"],"ISSN":["2314-7334"],"ESSN":["2314-7326"]}]}],"pubYear":["2016"],"abstractText":["Zika virus (ZIKV) is a member of the Flaviviridae family that had been associated only with mild disease prior to the 2015 outbreak in Brazil. A dramatic increase in reported cases of microcephaly and Guillain-Barré syndrome during this time prompted significant research into possible associations with ZIKV and its neurotropic properties. Infection of neural progenitor cells and organoids have been shown to induce apoptosis and dysregulation of growth, and mouse studies have demonstrated viral replication in brain tissue in adults, as well as vertical transmission resulting in embryonic brain abnormalities. Large case series of clinical and radiological findings of congenital ZIKV infection have begun to be published; however, pathology reports have been limited to two case reports and two small case series. Thus far, the findings have largely been restricted to the brain and include diffuse grey and white matter involvement consisting of dystrophic calcifications, gliosis, microglial nodules, neuronophagia, and scattered lymphocytes. Mild chronic villitis was observed in the placental tissue in some cases, and the remaining organs were essentially uninvolved. Larger, systematic studies, including correlation of histological findings with gestational age at the time of maternal infection, will be required to determine the full range of Zika virus-induced abnormalities and to help guide future clinical decision making."],"affiliation":["Department of Pathology, Brigham and Women's Hospital, Boston, USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982465?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982465"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-15"],"dateOfRevision":["2016-08-15"],"electronicPublicationDate":["2016-06-23"],"firstPublicationDate":["2016-06-23"],"cprojectID":"PMC4982465"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8JhOUS2orL1ISY","_score":1,"_source":{"id":["27540058"],"source":["MED"],"pmid":["27540058"],"pmcid":["PMC4991703"],"doi":["10.1128/genomea.00800-16"],"title":["Complete Genome Sequences of Three Historically Important, Spatiotemporally Distinct, and Genetically Divergent Strains of Zika Virus: MR-766, P6-740, and PRVABC-59."],"authorString":["Yun SI, Song BH, Frank JC, Julander JG, Polejaeva IA, Davies CJ, White KL, Lee YM."],"authorList":[{"author":[{"fullName":["Yun SI"],"firstName":["Sang-Im"],"lastName":["Yun"],"initials":["SI"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Song BH"],"firstName":["Byung-Hak"],"lastName":["Song"],"initials":["BH"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Frank JC"],"firstName":["Jordan C"],"lastName":["Frank"],"initials":["JC"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Julander JG"],"firstName":["Justin G"],"lastName":["Julander"],"initials":["JG"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Polejaeva IA"],"firstName":["Irina A"],"lastName":["Polejaeva"],"initials":["IA"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Davies CJ"],"firstName":["Christopher J"],"lastName":["Davies"],"initials":["CJ"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["White KL"],"firstName":["Kenneth L"],"lastName":["White"],"initials":["KL"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."]},{"fullName":["Lee YM"],"firstName":["Young-Min"],"lastName":["Lee"],"initials":["YM"],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA youngmin.lee@usu.edu."]}]}],"journalInfo":[{"issue":["4"],"volume":["4"],"journalIssueId":["2427904"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome announcements"],"ISOAbbreviation":["Genome Announc"],"medlineAbbreviation":["Genome Announc"],"NLMid":["101595808"],"ESSN":["2169-8287"]}]}],"pubYear":["2016"],"abstractText":["Here, we report the 10,807-nucleotide-long consensus RNA genome sequences of three spatiotemporally distinct and genetically divergent Zika virus strains, with the functionality of their genomic sequences substantiated by reverse genetics: MR-766 (African lineage, Uganda, 1947), P6-740 (Asian lineage, Malaysia, 1966), and PRVABC-59 (Asian lineage-derived American strain, Puerto Rico, 2015)."],"affiliation":["Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture and Applied Sciences, Utah State University, Logan, Utah, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991703?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991703"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00800-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-19"],"dateOfCreation":["2016-08-19"],"dateOfRevision":["2016-08-28"],"electronicPublicationDate":["2016-08-18"],"firstPublicationDate":["2016-08-18"],"cprojectID":"PMC4991703"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsAd_8VqgcuJwvkU","_score":1,"_source":{"id":["27563338"],"source":["MED"],"pmid":["27563338"],"pmcid":["PMC4977986"],"doi":["10.4103/1742-6413.187015"],"title":["CytoJournal Quiz Cases: Publishing open-access, PubMed-searchable cytopathology cases with educational messages."],"authorString":["Akers KG, Shidman VB."],"authorList":[{"author":[{"fullName":["Akers KG"],"firstName":["Katherine G"],"lastName":["Akers"],"initials":["KG"],"affiliation":["Address: Shiffman Medical Library, Wayne State University, Detroit, MI, USA."]},{"fullName":["Shidman VB"],"firstName":["Vinod B"],"lastName":["Shidman"],"initials":["VB"],"affiliation":["Department of Pathology, School of Medicine, Wayne State University, Detroit, MI, USA."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2384349"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["CytoJournal"],"ISOAbbreviation":["Cytojournal"],"medlineAbbreviation":["Cytojournal"],"NLMid":["101231642"],"ESSN":["1742-6413"]}]}],"pubYear":["2016"],"pageInfo":["15"],"affiliation":["Address: Shiffman Medical Library, Wayne State University, Detroit, MI, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4977986"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/1742-6413.187015"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-26"],"dateOfCreation":["2016-08-26"],"dateOfRevision":["2016-08-28"],"electronicPublicationDate":["2016-07-27"],"firstPublicationDate":["2016-07-27"],"cprojectID":"PMC4977986"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsBX_8VqgcuJwvkX","_score":1,"_source":{"id":["27606053"],"source":["MED"],"pmid":["27606053"],"pmcid":["PMC4979645"],"doi":["10.12688/f1000research.8546.1"],"title":["Perinatal neuroprotection update."],"authorString":["Jelin AC, Salmeen K, Gano D, Burd I, Thiet MP."],"authorList":[{"author":[{"fullName":["Jelin AC"],"firstName":["Angie C"],"lastName":["Jelin"],"initials":["AC"],"affiliation":["Department of Gynecology and Obstetrics, Johns Hopkins Hospital, Baltimore, MD, USA."]},{"fullName":["Salmeen K"],"firstName":["Kirsten"],"lastName":["Salmeen"],"initials":["K"],"affiliation":["Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Gano D"],"firstName":["Dawn"],"lastName":["Gano"],"initials":["D"],"affiliation":["Departments of Neurology & Pediatrics, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Burd I"],"firstName":["Irina"],"lastName":["Burd"],"initials":["I"],"affiliation":["Department of Gynecology and Obstetrics, Johns Hopkins Hospital, Baltimore, MD, USA."]},{"fullName":["Thiet MP"],"firstName":["Mari-Paule"],"lastName":["Thiet"],"initials":["MP"],"affiliation":["Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"abstractText":["Antepartum, intrapartum, and neonatal events can result in a spectrum of long-term neurological sequelae, including cerebral palsy, cognitive delay, schizophrenia, and autism spectrum disorders [1]. Advances in obstetrical and neonatal care have led to survival at earlier gestational ages and consequently increasing numbers of periviable infants who are at significant risk for long-term neurological deficits. Therefore, efforts to decrease and prevent cerebral insults attempt not only to decrease preterm delivery but also to improve neurological outcomes in infants delivered preterm. We recently published a comprehensive review addressing the impacts of magnesium sulfate, therapeutic hypothermia, delayed cord clamping, infections, and prevention of preterm delivery on the modification of neurological risk [2]. In this review, we will briefly provide updates to the aforementioned topics as well as an expansion on avoidance of toxin and infections, specifically the Zika virus."],"affiliation":["Department of Gynecology and Obstetrics, Johns Hopkins Hospital, Baltimore, MD, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Neurological deficits","Perinatal","neuroprotection","Preterm Delivery","Zika Virus","Delayed Umbilical Cord Clamping"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4979645?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4979645"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8546.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCompletion":["2016-09-08"],"dateOfCreation":["2016-09-08"],"electronicPublicationDate":["2016-08-09"],"firstPublicationDate":["2016-08-09"],"cprojectID":"PMC4979645"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDfhOUS2orL1ISl","_score":1,"_source":{"id":["27587826"],"source":["MED"],"pmid":["27587826"],"pmcid":["PMC5009983"],"doi":["10.1128/genomea.00917-16"],"title":["Genome Sequence of a Candidate World Health Organization Reference Strain of Zika Virus for Nucleic Acid Testing."],"authorString":["Trösemeier JH, Musso D, Blümel J, Thézé J, Pybus OG, Baylis SA."],"authorList":[{"author":[{"fullName":["Trösemeier JH"],"firstName":["Jan-Hendrik"],"lastName":["Trösemeier"],"initials":["JH"],"affiliation":["Paul-Ehrlich-Institut, Langen, Germany Molecular Bioinformatics, Johann Wolfgang Goethe University, Frankfurt am Main, Germany."]},{"fullName":["Musso D"],"firstName":["Didier"],"lastName":["Musso"],"initials":["D"],"affiliation":["Institut Louis Malardé, Tahiti, French Polynesia."]},{"fullName":["Blümel J"],"firstName":["Johannes"],"lastName":["Blümel"],"initials":["J"],"affiliation":["Paul-Ehrlich-Institut, Langen, Germany."]},{"fullName":["Thézé J"],"firstName":["Julien"],"lastName":["Thézé"],"initials":["J"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Pybus OG"],"firstName":["Oliver G"],"lastName":["Pybus"],"initials":["OG"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Baylis SA"],"firstName":["Sally A"],"lastName":["Baylis"],"initials":["SA"],"affiliation":["Paul-Ehrlich-Institut, Langen, Germany sally.baylis@pei.de."]}]}],"journalInfo":[{"issue":["5"],"volume":["4"],"journalIssueId":["2446031"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome announcements"],"ISOAbbreviation":["Genome Announc"],"medlineAbbreviation":["Genome Announc"],"NLMid":["101595808"],"ESSN":["2169-8287"]}]}],"pubYear":["2016"],"abstractText":["We report here the sequence of a candidate reference strain of Zika virus (ZIKV) developed on behalf of the World Health Organization (WHO). The ZIKV reference strain is intended for use in nucleic acid amplification (NAT)-based assays for the detection and quantification of ZIKV RNA."],"affiliation":["Paul-Ehrlich-Institut, Langen, Germany Molecular Bioinformatics, Johann Wolfgang Goethe University, Frankfurt am Main, Germany."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009983?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009983"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00917-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-09-02"],"dateOfCreation":["2016-09-02"],"dateOfRevision":["2016-09-07"],"electronicPublicationDate":["2016-09-01"],"firstPublicationDate":["2016-09-01"],"cprojectID":"PMC5009983"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDuJ9hGurOzZIcb","_score":1,"_source":{"id":["27128312"],"source":["MED"],"pmid":["27128312"],"pmcid":["PMC4851397"],"doi":["10.1371/journal.pntd.0004681"],"title":["Predicting Dengue Fever Outbreaks in French Guiana Using Climate Indicators."],"authorString":["Adde A, Roucou P, Mangeas M, Ardillon V, Desenclos JC, Rousset D, Girod R, Briolant S, Quenel P, Flamand C."],"authorList":[{"author":[{"fullName":["Adde A"],"firstName":["Antoine"],"lastName":["Adde"],"initials":["A"],"affiliation":["Unité d'entomologie médicale, Institut Pasteur de la Guyane, Cayenne, Guyane."]},{"fullName":["Roucou P"],"firstName":["Pascal"],"lastName":["Roucou"],"initials":["P"],"affiliation":["Centre de Recherches de Climatologie, UMR6282 Biogéosciences, CNRS Université de Bourgogne Franche-Comté, Dijon, France."]},{"fullName":["Mangeas M"],"firstName":["Morgan"],"lastName":["Mangeas"],"initials":["M"],"affiliation":["Maison de la Télédétection, UMR 228 ESPACE-DEV, Institut de Recherche pour le Développement, Montpellier, France."]},{"fullName":["Ardillon V"],"firstName":["Vanessa"],"lastName":["Ardillon"],"initials":["V"],"affiliation":["Cellule de l'Institut de Veille Sanitaire en Régions Antilles - Guyane, Cayenne, Guyane."]},{"fullName":["Desenclos JC"],"firstName":["Jean-Claude"],"lastName":["Desenclos"],"initials":["JC"],"affiliation":["Institut de Veille Sanitaire, Saint-Maurice, France."]},{"fullName":["Rousset D"],"firstName":["Dominique"],"lastName":["Rousset"],"initials":["D"],"affiliation":["Unité de virologie, Institut Pasteur de la Guyane, Cayenne, Guyane."]},{"fullName":["Girod R"],"firstName":["Romain"],"lastName":["Girod"],"initials":["R"],"affiliation":["Unité d'entomologie médicale, Institut Pasteur de la Guyane, Cayenne, Guyane."]},{"fullName":["Briolant S"],"firstName":["Sébastien"],"lastName":["Briolant"],"initials":["S"],"affiliation":["Institut de Recherche Biomédicale des Armées, Brétigny sur Orge, France."]},{"fullName":["Quenel P"],"firstName":["Philippe"],"lastName":["Quenel"],"initials":["P"],"affiliation":["Laboratoire d'Etudes et de Recherche en Santé-Environnement, Ecole des Hautes Etudes en Santé Publique, Rennes, France."]},{"fullName":["Flamand C"],"firstName":["Claude"],"lastName":["Flamand"],"initials":["C"],"affiliation":["Unité d'épidémiologie, Institut Pasteur de la Guyane, Cayenne, Guyane."]}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004681"],"abstractText":["Dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. Associations between climate and dengue have been studied around the world, but the results have shown that the impact of the climate can vary widely from one study site to another. In French Guiana, climate-based models are not available to assist in developing an early warning system. This study aims to evaluate the potential of using oceanic and atmospheric conditions to help predict dengue fever outbreaks in French Guiana.Lagged correlations and composite analyses were performed to identify the climatic conditions that characterized a typical epidemic year and to define the best indices for predicting dengue fever outbreaks during the period 1991-2013. A logistic regression was then performed to build a forecast model. We demonstrate that a model based on summer Equatorial Pacific Ocean sea surface temperatures and Azores High sea-level pressure had predictive value and was able to predict 80% of the outbreaks while incorrectly predicting only 15% of the non-epidemic years. Predictions for 2014-2015 were consistent with the observed non-epidemic conditions, and an outbreak in early 2016 was predicted.These findings indicate that outbreak resurgence can be modeled using a simple combination of climate indicators. This might be useful for anticipating public health actions to mitigate the effects of major outbreaks, particularly in areas where resources are limited and medical infrastructures are generally insufficient."],"affiliation":["Unité d'entomologie médicale, Institut Pasteur de la Guyane, Cayenne, Guyane."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Epidemiologic Methods"]},{"majorTopic_YN":["Y"],"descriptorName":["Models, Statistical"]},{"majorTopic_YN":["Y"],"descriptorName":["Climate"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Forecasting"]},{"majorTopic_YN":["N"],"descriptorName":["French Guiana"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4851397?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4851397"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004681"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-30"],"dateOfRevision":["2016-05-09"],"electronicPublicationDate":["2016-04-29"],"firstPublicationDate":["2016-04-29"],"cprojectID":"PMC4851397"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsEohOUS2orL1ISq","_score":1,"_source":{"id":["27564232"],"source":["MED"],"pmid":["27564232"],"pmcid":["PMC5001720"],"doi":["10.1371/journal.pntd.0004968"],"title":["An Ecological Assessment of the Pandemic Threat of Zika Virus."],"authorString":["Carlson CJ, Dougherty ER, Getz W."],"authorList":[{"author":[{"fullName":["Carlson CJ"],"firstName":["Colin J"],"lastName":["Carlson"],"initials":["CJ"],"affiliation":["Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Dougherty ER"],"firstName":["Eric R"],"lastName":["Dougherty"],"initials":["ER"],"affiliation":["Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Getz W"],"firstName":["Wayne"],"lastName":["Getz"],"initials":["W"],"affiliation":["School of Mathematical Sciences, University of KwaZulu-Natal, Durban, South Africa."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004968"],"abstractText":["The current outbreak of Zika virus poses a severe threat to human health. While the range of the virus has been cataloged growing slowly over the last 50 years, the recent explosive expansion in the Americas indicates that the full potential distribution of Zika remains uncertain. Moreover, many studies rely on its similarity to dengue fever, a phylogenetically closely related disease of unknown ecological comparability. Here we compile a comprehensive spatially-explicit occurrence dataset from Zika viral surveillance and serological surveys based in its native range, and construct ecological niche models to test basic hypotheses about its spread and potential establishment. The hypothesis that the outbreak of cases in Mexico and North America are anomalous and outside the native ecological niche of the disease, and may be linked to either genetic shifts between strains, or El Nino or similar climatic events, remains plausible at this time. Comparison of the Zika niche against the known distribution of dengue fever suggests that Zika is more constrained by the seasonality of precipitation and diurnal temperature fluctuations, likely confining autochthonous non-sexual transmission to the tropics without significant evolutionary change. Projecting the range of the diseases in conjunction with three major vector species (Aedes africanus, Ae. aegypti, and Ae. albopictus) that transmit the pathogens, under climate change, suggests that Zika has potential for northward expansion; but, based on current knowledge, our models indicate Zika is unlikely to fill the full range its vectors occupy, and public fear of a vector-borne Zika epidemic in the mainland United States is potentially informed by biased or limited scientific knowledge. With recent sexual transmission of the virus globally, we caution that our results only apply to the vector-borne transmission route of the pathogen, and while the threat of a mosquito-carried Zika pandemic may be overstated in the media, other transmission modes of the virus may emerge and facilitate naturalization worldwide."],"affiliation":["Department of Environmental Science, Policy, and Management, University of California, Berkeley, Berkeley, California, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5001720?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5001720"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004968"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-27"],"electronicPublicationDate":["2016-08-26"],"firstPublicationDate":["2016-08-26"],"cprojectID":"PMC5001720"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrBd_8VqgcuJwvhN","_score":1,"_source":{"id":["26918158"],"source":["MED"],"pmid":["26918158"],"pmcid":["PMC4754000"],"doi":["10.12688/f1000research.7171.1"],"title":["Chikungunya: epidemiology."],"authorString":["Petersen LR, Powers AM."],"authorList":[{"author":[{"fullName":["Petersen LR"],"firstName":["Lyle R"],"lastName":["Petersen"],"initials":["LR"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA."]},{"fullName":["Powers AM"],"firstName":["Ann M"],"lastName":["Powers"],"initials":["AM"],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2369080"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"abstractText":["Chikungunya virus is a mosquito-borne alphavirus that causes fever and debilitating joint pains in humans. Joint pains may last months or years. It is vectored primarily by the tropical and sub-tropical mosquito, Aedes aegypti, but is also found to be transmitted by Aedes albopictus, a mosquito species that can also be found in more temperate climates. In recent years, the virus has risen from relative obscurity to become a global public health menace affecting millions of persons throughout the tropical and sub-tropical world and, as such, has also become a frequent cause of travel-associated febrile illness. In this review, we discuss our current understanding of the biological and sociological underpinnings of its emergence and its future global outlook."],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Alphavirus","Mosquito","Chikungunya"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4754000?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4754000"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.7171.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-02-26"],"dateOfCreation":["2016-02-26"],"dateOfRevision":["2016-03-01"],"electronicPublicationDate":["2016-01-19"],"firstPublicationDate":["2016-01-19"],"cprojectID":"PMC4754000"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrHvJ9hGurOzZIZY","_score":1,"_source":{"id":["26399159"],"source":["MED"],"pmid":["26399159"],"pmcid":["PMC4829329"],"doi":["10.1080/15476286.2015.1094599"],"title":["New hypotheses derived from the structure of a flaviviral Xrn1-resistant RNA: Conservation, folding, and host adaptation."],"authorString":["Kieft JS, Rabe JL, Chapman EG."],"authorList":[{"author":[{"fullName":["Kieft JS"],"firstName":["Jeffrey S"],"lastName":["Kieft"],"initials":["JS"],"affiliation":["b Howard Hughes Medical Institute; University of Colorado Denver; School of Medicine ; Aurora , CO USA."]},{"fullName":["Rabe JL"],"firstName":["Jennifer L"],"lastName":["Rabe"],"initials":["JL"],"affiliation":["c Department of Pediatrics ; University of Colorado Denver; School of Medicine ; Aurora , CO USA."]},{"fullName":["Chapman EG"],"firstName":["Erich G"],"lastName":["Chapman"],"initials":["EG"],"affiliation":["a Department of Biochemistry and Molecular Genetics."]}]}],"journalInfo":[{"issue":["11"],"volume":["12"],"journalIssueId":["2387062"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["RNA biology"],"ISOAbbreviation":["RNA Biol"],"medlineAbbreviation":["RNA Biol"],"NLMid":["101235328"],"ISSN":["1547-6286"],"ESSN":["1555-8584"]}]}],"pubYear":["2015"],"pageInfo":["1169-1177"],"abstractText":["Arthropod-borne flaviviruses (FVs) are a growing world-wide health threat whose incidence and range are increasing. The pathogenicity and cytopathicity of these single-stranded RNA viruses are influenced by viral subgenomic non-protein-coding RNAs (sfRNAs) that the viruses produce to high levels during infection. To generate sfRNAs the virus co-opts the action of the abundant cellular exonuclease Xrn1, which is part of the cell's normal RNA turnover machinery. This exploitation of the cellular machinery is enabled by discrete, highly structured, Xrn1-resistant RNA elements (xrRNAs) in the 3'UTR that interact with Xrn1 to halt processive 5' to 3' decay of the viral genomic RNA. We recently solved the crystal structure of a functional xrRNA, revealing a novel fold that provides a mechanistic model for Xrn1 resistance. Continued analysis and interpretation of the structure reveals that the tertiary contacts that knit the xrRNA fold together are shared by a wide variety of arthropod-borne FVs, conferring robust Xrn1 resistance in all tested. However, there is some variability in the structures that correlates with unexplained patterns in the viral 3' UTRs. Finally, examination of these structures and their behavior in the context of viral infection leads to a new hypothesis linking RNA tertiary structure, overall 3' UTR architecture, sfRNA production, and host adaptation."],"affiliation":["b Howard Hughes Medical Institute; University of Colorado Denver; School of Medicine ; Aurora , CO USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["1F32GM108257"],"agency":["NIGMS NIH HHS"],"acronym":["GM"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["RNA structure","RNA folding","Sfrna","Flaviviral Rna","Xrn1 Resistance"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4829329?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4829329"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1080/15476286.2015.1094599"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-12-01"],"dateOfRevision":["2016-05-05"],"electronicPublicationDate":["2015-09-23"],"firstPublicationDate":["2015-09-23"],"cprojectID":"PMC4829329"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrH0_8VqgcuJwvhb","_score":1,"_source":{"id":["27014219"],"source":["MED"],"pmid":["27014219"],"pmcid":["PMC4779909"],"doi":["10.3389/fmicb.2016.00296"],"title":["Inhibition of West Nile Virus Multiplication in Cell Culture by Anti-Parkinsonian Drugs."],"authorString":["Blázquez AB, Martín-Acebes MA, Saiz JC."],"authorList":[{"author":[{"fullName":["Blázquez AB"],"firstName":["Ana B"],"lastName":["Blázquez"],"initials":["AB"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Martín-Acebes MA"],"firstName":["Miguel A"],"lastName":["Martín-Acebes"],"initials":["MA"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]},{"fullName":["Saiz JC"],"firstName":["Juan-Carlos"],"lastName":["Saiz"],"initials":["JC"],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2369092"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2016"],"pageInfo":["296"],"abstractText":["West Nile virus (WNV) is a mosquito-borne flavivirus maintained in a transmission cycle between mosquitoes and birds, but it can also infect other vertebrates, including humans, in which it can cause neuroinvasive diseases. To date, no licensed vaccine or therapy for human use against this pathogen is yet available. A recent approach to search for new antiviral agent candidates is the assessment of long-used drugs commonly administered by clinicians to treat human disorders in drug antiviral development. In this regard, as patients with West Nile encephalitis frequently develop symptoms and features of parkinsonism, and cellular factors altered in parkinsonism, such as alpha-synuclein, have been shown to play a role on WNV infection, we have assessed the effect of four drugs (L-dopa, Selegiline, Isatin, and Amantadine), that are used as therapy for Parkinson's disease in the inhibition of WNV multiplication. L-dopa, Isatin, and Amantadine treatments significantly reduced the production of infectious virus in all cell types tested, but only Amantadine reduced viral RNA levels. These results point to antiparkinsonian drugs as possible therapeutic candidates for the development of antiviral strategies against WNV infection."],"affiliation":["Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Madrid, Spain."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Inhibition","West Nile virus","Antivirals","Flavivirus","Parkinson","Neuroinvasive Disease"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4779909?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4779909"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2016.00296"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-03-25"],"dateOfCreation":["2016-03-25"],"dateOfRevision":["2016-03-27"],"electronicPublicationDate":["2016-03-07"],"firstPublicationDate":["2016-03-07"],"cprojectID":"PMC4779909"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrIkhOUS2orL1IPn","_score":1,"_source":{"id":["26956448"],"source":["MED"],"pmid":["26956448"],"pmcid":["PMC4820676"],"doi":["10.1038/emi.2016.42"],"title":["Zika virus outbreak: 'a perfect storm'."],"authorString":["Ai JW, Zhang Y, Zhang W."],"authorList":[{"author":[{"fullName":["Ai JW"],"firstName":["Jing-Wen"],"lastName":["Ai"],"initials":["JW"],"affiliation":["Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China."]},{"fullName":["Zhang Y"],"firstName":["Ying"],"lastName":["Zhang"],"initials":["Y"],"affiliation":["Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA."]},{"fullName":["Zhang W"],"firstName":["Wenhong"],"lastName":["Zhang"],"initials":["W"],"affiliation":["MOH and MOE Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2370343"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Emerging microbes & infections"],"ISOAbbreviation":["Emerg Microbes Infect"],"medlineAbbreviation":["Emerg Microbes Infect"],"NLMid":["101594885"],"ESSN":["2222-1751"]}]}],"pubYear":["2016"],"pageInfo":["e21"],"affiliation":["Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4820676?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4820676"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/emi.2016.42"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-09"],"dateOfRevision":["2016-04-18"],"electronicPublicationDate":["2016-03-09"],"firstPublicationDate":["2016-03-09"],"cprojectID":"PMC4820676"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrJs_8VqgcuJwvhg","_score":1,"_source":{"id":["26986061"],"source":["MED"],"pmid":["26986061"],"pmcid":["PMC4795698"],"doi":["10.1371/journal.pntd.0004516"],"title":["Pre-cut Filter Paper for Detecting Anti-Japanese Encephalitis Virus IgM from Dried Cerebrospinal Fluid Spots."],"authorString":["Bharucha T, Chanthongthip A, Phuangpanom S, Phonemixay O, Sengvilaipaseuth O, Vongsouvath M, Lee S, Newton PN, Dubot-Pérès A."],"authorList":[{"author":[{"fullName":["Bharucha T"],"firstName":["Tehmina"],"lastName":["Bharucha"],"initials":["T"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Chanthongthip A"],"firstName":["Anisone"],"lastName":["Chanthongthip"],"initials":["A"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Phuangpanom S"],"firstName":["Soumphou"],"lastName":["Phuangpanom"],"initials":["S"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Phonemixay O"],"firstName":["Ooyanong"],"lastName":["Phonemixay"],"initials":["O"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Sengvilaipaseuth O"],"firstName":["Onanong"],"lastName":["Sengvilaipaseuth"],"initials":["O"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Vongsouvath M"],"firstName":["Manivanh"],"lastName":["Vongsouvath"],"initials":["M"],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."]},{"fullName":["Lee S"],"firstName":["Sue"],"lastName":["Lee"],"initials":["S"],"affiliation":["Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand."]},{"fullName":["Newton PN"],"firstName":["Paul N"],"lastName":["Newton"],"initials":["PN"],"affiliation":["Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom."]},{"fullName":["Dubot-Pérès A"],"firstName":["Audrey"],"lastName":["Dubot-Pérès"],"initials":["A"],"affiliation":["UMR_D 190 \"Emergence des Pathologies Virales\" (Aix-Marseille Univ, IRD French Institute of Research for Development, EHESP French School of Public Health), Marseille, France."]}]}],"journalInfo":[{"issue":["3"],"volume":["10"],"journalIssueId":["2380932"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004516"],"abstractText":["The use of filter paper as a simple, inexpensive tool for storage and transportation of blood, 'Dried Blood Spots' or Guthrie cards, for diagnostic assays is well-established. In contrast, there are a paucity of diagnostic evaluations of dried cerebrospinal fluid (CSF) spots. These have potential applications in low-resource settings, such as Laos, where laboratory facilities for central nervous system (CNS) diagnostics are only available in Vientiane. In Laos, a major cause of CNS infection is Japanese encephalitis virus (JEV). We aimed to develop a dried CSF spot protocol and to evaluate its diagnostic performance using the World Health Organisation recommended anti-JEV IgM antibody capture enzyme-linked immunosorbent assay (JEV MAC-ELISA).Sample volumes, spotting techniques and filter paper type were evaluated using a CSF-substitute of anti-JEV IgM positive serum diluted in Phosphate Buffer Solution (PBS) to end-limits of detection by JEV MAC-ELISA. A conventional protocol, involving eluting one paper punch in 200 μl PBS, did not detect the end-dilution, nor did multiple punches utilising diverse spotting techniques. However, pre-cut filter paper enabled saturation with five times the volume of CSF-substitute, sufficiently improving sensitivity to detect the end-dilution. The diagnostic accuracy of this optimised protocol was compared with routine, neat CSF in a pilot, retrospective study of JEV MAC-ELISA on consecutive CSF samples, collected 2009-15, from three Lao hospitals. In comparison to neat CSF, 132 CSF samples stored as dried CSF spots for one month at 25-30 °C showed 81.6% (65.7-92.3 95%CI) positive agreement, 96.8% (91.0-99.3 95%CI) negative agreement, with a kappa coefficient of 0.81 (0.70-0.92 95%CI).The novel design of pre-cut filter paper saturated with CSF could provide a useful tool for JEV diagnostics in settings with limited laboratory access. It has the potential to improve national JEV surveillance and inform vaccination policies. The saturation of filter paper has potential use in the wider context of pathogen detection, including dried spots for detecting other analytes in CSF, and other body fluids."],"affiliation":["Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't","Comparative Study"]}],"grantsList":[{"grant":[{"agency":["Wellcome Trust"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Cerebrospinal Fluid"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mycobacterium"]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis Virus, Japanese"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Encephalitis, Japanese"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin M"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CF"],"qualifierName":["cerebrospinal fluid"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CF"],"qualifierName":["cerebrospinal fluid"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Specimen Handling"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Desiccation"]},{"majorTopic_YN":["N"],"descriptorName":["Retrospective Studies"]},{"majorTopic_YN":["N"],"descriptorName":["Pilot Projects"]},{"majorTopic_YN":["N"],"descriptorName":["Temperature"]},{"majorTopic_YN":["Y"],"descriptorName":["Paper"]},{"majorTopic_YN":["N"],"descriptorName":["Laos"]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["Immunoglobulin M"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4795698?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4795698"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004516"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-03-18"],"dateOfRevision":["2016-03-24"],"electronicPublicationDate":["2016-03-17"],"firstPublicationDate":["2016-03-17"],"cprojectID":"PMC4795698"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrNIJ9hGurOzZIZn","_score":1,"_source":{"id":["27101839"],"source":["MED"],"pmid":["27101839"],"pmcid":["PMC4840257"],"doi":["10.1051/parasite/2016018"],"title":["An updated checklist of mosquito species (Diptera: Culicidae) from Madagascar."],"authorString":["Tantely ML, Le Goff G, Boyer S, Fontenille D."],"authorList":[{"author":[{"fullName":["Tantely ML"],"firstName":["Michaël Luciano"],"lastName":["Tantely"],"initials":["ML"],"affiliation":["Laboratoire d'Entomologie Médicale, Institut Pasteur de Madagascar, Antananarivo 101, Madagascar."]},{"fullName":["Le Goff G"],"firstName":["Gilbert"],"lastName":["Le Goff"],"initials":["G"],"affiliation":["IRD UMR MIVEGEC, 34394 Montpellier and IRD La Réunion-GIP CYROI, 97490 Sainte Clotilde, La Réunion, France."]},{"fullName":["Boyer S"],"firstName":["Sébastien"],"lastName":["Boyer"],"initials":["S"],"affiliation":["Laboratoire d'Entomologie Médicale, Institut Pasteur de Madagascar, Antananarivo 101, Madagascar."]},{"fullName":["Fontenille D"],"firstName":["Didier"],"lastName":["Fontenille"],"initials":["D"],"affiliation":["IRD UMR MIVEGEC, 34394 Montpellier and Institut Pasteur du Cambodge 5, BP 983, Blvd. Monivong, 12201 Phnom Penh, Cambodia."]}]}],"journalInfo":[{"volume":["23"],"journalIssueId":["2366248"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasite (Paris, France)"],"ISOAbbreviation":["Parasite"],"medlineAbbreviation":["Parasite"],"NLMid":["9437094"],"ISSN":["1252-607X"],"ESSN":["1776-1042"]}]}],"pubYear":["2016"],"pageInfo":["20"],"abstractText":["An updated checklist of 235 mosquito species from Madagascar is presented. The number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). This annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. The 235 species belong to 14 genera: Aedeomyia (3 species), Aedes (35 species), Anopheles (26 species), Coquillettidia (3 species), Culex (at least 50 species), Eretmapodites (4 species), Ficalbia (2 species), Hodgesia (at least one species), Lutzia (one species), Mansonia (2 species), Mimomyia (22 species), Orthopodomyia (8 species), Toxorhynchites (6 species), and Uranotaenia (73 species). Due to non-deciphered species complexes, several species remain undescribed. The main remarkable characteristic of Malagasy mosquito fauna is the high biodiversity with 138 endemic species (59%). Presence and abundance of species, and their association, in a given location could be a bio-indicator of environmental particularities such as urban, rural, forested, deforested, and mountainous habitats. Finally, taking into account that Malagasy culicidian fauna includes 64 species (27%) with a known medical or veterinary interest in the world, knowledge of their biology and host preference summarized in this paper improves understanding of their involvement in pathogen transmission in Madagascar."],"affiliation":["Laboratoire d'Entomologie Médicale, Institut Pasteur de Madagascar, Antananarivo 101, Madagascar."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4840257?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4840257"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1051/parasite/2016018"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-22"],"dateOfRevision":["2016-05-18"],"electronicPublicationDate":["2016-04-21"],"firstPublicationDate":["2016-04-21"],"cprojectID":"PMC4840257"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrNdhOUS2orL1IP1","_score":1,"_source":{"id":["27102015"],"source":["MED"],"pmid":["27102015"],"pmcid":["PMC4839143"],"doi":["10.1186/s13071-016-1501-x"],"title":["Enhancement of Aedes albopictus collections by ovitrap and sticky adult trap."],"authorString":["Velo E, Kadriaj P, Mersini K, Shukullari A, Manxhari B, Simaku A, Hoxha A, Caputo B, Bolzoni L, Rosà R, Bino S, Reiter P, della Torre A."],"authorList":[{"author":[{"fullName":["Velo E"],"firstName":["Enkelejda"],"lastName":["Velo"],"initials":["E"],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania. keladikolli@yahoo.com."]},{"fullName":["Kadriaj P"],"firstName":["Perparim"],"lastName":["Kadriaj"],"initials":["P"],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania."]},{"fullName":["Mersini K"],"firstName":["Kujtim"],"lastName":["Mersini"],"initials":["K"],"affiliation":["National Veterinary Epidemiology Unit, Food Safety and Veterinary Institute, Tirana, Albania."]},{"fullName":["Shukullari A"],"firstName":["Ada"],"lastName":["Shukullari"],"initials":["A"],"affiliation":["Department of Biology, Faculty of Natural Sciences, Tirana, Albania."]},{"fullName":["Manxhari B"],"firstName":["Blerta"],"lastName":["Manxhari"],"initials":["B"],"affiliation":["Department of Biology, Faculty of Natural Sciences, Tirana, Albania."]},{"fullName":["Simaku A"],"firstName":["Artan"],"lastName":["Simaku"],"initials":["A"],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania."]},{"fullName":["Hoxha A"],"firstName":["Adrian"],"lastName":["Hoxha"],"initials":["A"],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania."]},{"fullName":["Caputo B"],"firstName":["Beniamino"],"lastName":["Caputo"],"initials":["B"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Rome, Italy."]},{"fullName":["Bolzoni L"],"firstName":["Luca"],"lastName":["Bolzoni"],"initials":["L"],"affiliation":["Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Parma, Italy."]},{"fullName":["Rosà R"],"firstName":["Roberto"],"lastName":["Rosà"],"initials":["R"],"authorId":[{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}],"affiliation":["Dipartimento di Biodiversità ed Ecologia Molecolare, Centro Ricerca e Innovazione, Fondazione Edmund Mach, San Michele all'Adige, Trento, Italy."]},{"fullName":["Bino S"],"firstName":["Silvia"],"lastName":["Bino"],"initials":["S"],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania."]},{"fullName":["Reiter P"],"firstName":["Paul"],"lastName":["Reiter"],"initials":["P"],"affiliation":["Insects and Infectious Disease Unit, Institute Pasteur, Paris, France."]},{"fullName":["della Torre A"],"firstName":["Alessandra"],"lastName":["della Torre"],"initials":["A"],"affiliation":["Dipartimento di Sanità Pubblica e Malattie Infettive, Università di Roma \"Sapienza\", Rome, Italy."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8655-2230","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2401389"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["223"],"abstractText":["In the last decades, Aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which Dengue, Chikungunya and Zika. Enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control activities.Two sets of 4 × 4 Latin-square experiments were carried out in Tirana (Albania) to test whether modifications in ovitrap shape and size and in oviposition substrate would increase collections of Ae. albopictus eggs and whether hay-infusion would increase adult catches by sticky trap. Generalized Linear Mixed Models with negative binomial error distribution were carried out to analyse the data. Cylindrical ovitraps lined with germination paper yielded significantly higher egg catches than those exploiting either the (commonly used) wooden paddles or floating polystyrene blocks as oviposition substrates. No difference was observed between cylindrical and conical shaped ovitraps. Ovitraps and sticky traps baited with hay infusion yielded significantly higher egg and adult catches than un-baited ones. A significant relationship between ovitrap and sticky trap catches was observed both in the absence and in the presence of attractants, with ovitrap catches increasing more than sticky trap catches at increasing adult female densities.This study provides grounds for optimisation of ovitraps and sticky traps as monitoring tools for Ae. albopictus by (i) supporting use of germination paper as most appropriate oviposition substrate; (ii) suggesting the possible use of stackable conical ovitraps for large scale monitoring; (iii) confirming the use of hay-infusion to increase egg catches in ovitraps, and showing that hay-infusion also significant increases adult catches by sticky traps."],"affiliation":["Control of Infectious Diseases Department, Institute of Public Health, Tirana, Albania. keladikolli@yahoo.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Aedes","sticky trap","Europe","Ovitrap","Albania","Mosquito monitoring","Mosquito Surveillance","Hay-infusion"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839143?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839143"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1501-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-22"],"dateOfRevision":["2016-04-24"],"electronicPublicationDate":["2016-04-21"],"firstPublicationDate":["2016-04-21"],"cprojectID":"PMC4839143"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrOkhOUS2orL1IP5","_score":1,"_source":{"id":["27100624"],"source":["MED"],"pmid":["27100624"],"pmcid":["PMC4839776"],"doi":["10.1371/journal.pone.0153573"],"title":["Solvent/Detergent Virally Inactivated Serum Eye Drops Restore Healthy Ocular Epithelium in a Rabbit Model of Dry-Eye Syndrome."],"authorString":["Tseng CL, Chen ZY, Renn TY, Hsiao SH, Burnouf T."],"authorList":[{"author":[{"fullName":["Tseng CL"],"firstName":["Ching-Li"],"lastName":["Tseng"],"initials":["CL"],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."]},{"fullName":["Chen ZY"],"firstName":["Zhi-Yu"],"lastName":["Chen"],"initials":["ZY"],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."]},{"fullName":["Renn TY"],"firstName":["Ting-Yi"],"lastName":["Renn"],"initials":["TY"],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."]},{"fullName":["Hsiao SH"],"firstName":["Shun-Hung"],"lastName":["Hsiao"],"initials":["SH"],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."]},{"fullName":["Burnouf T"],"firstName":["Thierry"],"lastName":["Burnouf"],"initials":["T"],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."]}]}],"journalInfo":[{"issue":["4"],"volume":["11"],"journalIssueId":["2392598"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0153573"],"abstractText":["Application of autologous serum eye drops (SEDs) is a recognized means to treat severe dry-eye syndrome (DES). Due to the inconvenience and difficulty of preparing SEDs from some patients, producing SEDs from allogeneic blood donations is gaining popularity. A major safety concern associated with allogeneic blood is virus transmission. We therefore herein evaluated the possibility of applying a solvent/detergent (S/D) treatment to inactivate viruses and studied the impacts of such treatment of SEDs to resolve DES in a rabbit model. Sera prepared from the blood of five rabbits were pooled and divided into two sub-pools. One was untreated (SEDs), while the other was virally-inactivated with 1% Tri-n-butyl phosphate/1% Triton X-45 at 31°C for 1 h (S/D-SEDs). DES was induced in rabbits using 0.1% benzalkonium chloride (BAC). Rabbits were divided into five groups of two rabbits each. One group was untreated (control), three were treated twice daily for 3 weeks using PBS, SEDs, or S/D-SEDs, and the last received an additional 0.1% BAC (as the negative control). The DES condition was determined by measuring aqueous tear secretion (Schirmer's test), corneal fluorescein staining, a corneal histologic examination, TUNEL stain apoptosis, and corneal inflammatory marker (tumor necrosis factor-α, interleukin (IL)-1β, IL-8, and IL-6) expressions. We first confirmed that SEDs and S/D-SEDs had similar protein profiles and transforming growth factor (TGF)-β contents. Animal experiments showed that tear secretion did not significantly differ between the SED and S/D-SED groups but was significantly higher than in the PBS group. Eye fluorescein staining revealed dramatic improvements in epithelial defects in groups treated with SEDs or S/D-SEDs, and hematoxylin/eosin staining revealed microscopic epithelial layers similar to those of the untreated controls. Inflammatory markers and TUNEL studies showed that healthy epithelium had been restored in groups treated with SEDs or S/D-SEDs. In conclusion, this preclinical study supports the possibility of using S/D virally inactivated SEDs to treat DES and restore a normal epithelium."],"affiliation":["Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Cornea"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CY"],"qualifierName":["cytology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Epithelium"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Serum"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Rabbits"]},{"majorTopic_YN":["N"],"descriptorName":["Dry Eye Syndromes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CI"],"qualifierName":["chemically induced"],"majorTopic_YN":["N"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["PC"],"qualifierName":["prevention & control"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Inflammation Mediators"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cytokines"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ophthalmic Solutions"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AD"],"qualifierName":["administration & dosage"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Solvents"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Detergents"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Virus Inactivation"]},{"majorTopic_YN":["N"],"descriptorName":["Disinfection"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]}]}],"chemicalList":[{"chemical":[{"name":["Solvents"],"registryNumber":["0"]},{"name":["Detergents"],"registryNumber":["0"]},{"name":["Ophthalmic Solutions"],"registryNumber":["0"]},{"name":["Cytokines"],"registryNumber":["0"]},{"name":["Inflammation Mediators"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839776?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839776"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0153573"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-09-02"],"dateOfCreation":["2016-04-22"],"dateOfRevision":["2016-04-30"],"electronicPublicationDate":["2016-04-21"],"firstPublicationDate":["2016-04-21"],"cprojectID":"PMC4839776"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSbhOUS2orL1IN3","_score":1,"_source":{"id":["18218114"],"source":["MED"],"pmid":["18218114"],"pmcid":["PMC2263041"],"doi":["10.1186/1743-422x-5-16"],"title":["Universal primers that amplify RNA from all three flavivirus subgroups."],"authorString":["Maher-Sturgess SL, Forrester NL, Wayper PJ, Gould EA, Hall RA, Barnard RT, Gibbs MJ."],"authorList":[{"author":[{"fullName":["Maher-Sturgess SL"],"firstName":["Sheryl L"],"lastName":["Maher-Sturgess"],"initials":["SL"]},{"fullName":["Forrester NL"],"firstName":["Naomi L"],"lastName":["Forrester"],"initials":["NL"]},{"fullName":["Wayper PJ"],"firstName":["Paul J"],"lastName":["Wayper"],"initials":["PJ"]},{"fullName":["Gould EA"],"firstName":["Ernest A"],"lastName":["Gould"],"initials":["EA"]},{"fullName":["Hall RA"],"firstName":["Roy A"],"lastName":["Hall"],"initials":["RA"]},{"fullName":["Barnard RT"],"firstName":["Ross T"],"lastName":["Barnard"],"initials":["RT"],"authorId":[{"_":"0000-0002-5685-4828","$":{"type":"ORCID"}}]},{"fullName":["Gibbs MJ"],"firstName":["Mark J"],"lastName":["Gibbs"],"initials":["MJ"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5685-4828","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["1508293"],"dateOfPublication":["2008 "],"monthOfPublication":["0"],"yearOfPublication":["2008"],"printPublicationDate":["2008-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2008"],"pageInfo":["16"],"abstractText":[" BACKGROUND: Species within the Flavivirus genus pose public health problems around the world. Increasing cases of Dengue and Japanese encephalitis virus in Asia, frequent outbreaks of Yellow fever virus in Africa and South America, and the ongoing spread of West Nile virus throughout the Americas, show the geographical burden of flavivirus diseases. Flavivirus infections are often indistinct from and confused with other febrile illnesses. Here we review the specificity of published primers, and describe a new universal primer pair that can detect a wide range of flaviviruses, including viruses from each of the recognised subgroups. RESULTS: Bioinformatic analysis of 257 published full-length Flavivirus genomes revealed conserved regions not previously targeted by primers. Two degenerate primers, Flav100F and Flav200R were designed from these regions and used to generate an 800 base pair cDNA product. The region amplified encoded part of the methyltransferase and most of the RNA-dependent-RNA-polymerase (NS5) coding sequence. One-step RT-PCR testing was successful using standard conditions with RNA from over 60 different flavivirus strains representing about 50 species. The cDNA from each virus isolate was sequenced then used in phylogenetic analyses and database searches to confirm the identity of the template RNA. CONCLUSION: Comprehensive testing has revealed the broad specificity of these primers. We briefly discuss the advantages and uses of these universal primers."],"affiliation":["Australian Biosecurity CRC, University of Queensland, St Lucia, QLD 4067, Australia. Sheryl.Maher@gmail.com"],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"grantId":["ceh010021"],"agency":["Natural Environment Research Council"],"orderIn":["0"]},{"grantId":["CEH010021"],"agency":["Natural Environment Research Council"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Nonstructural Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BI"],"qualifierName":["biosynthesis"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["DNA Primers"]},{"majorTopic_YN":["Y"],"descriptorName":["Nucleic Acid Amplification Techniques"]},{"majorTopic_YN":["N"],"descriptorName":["Conserved Sequence"]}]}],"chemicalList":[{"chemical":[{"name":["NS5 protein, flavivirus"],"registryNumber":["0"]},{"name":["RNA, Viral"],"registryNumber":["0"]},{"name":["DNA Primers"],"registryNumber":["0"]},{"name":["Viral Nonstructural Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2263041?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2263041"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=18218114"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=18218114&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1743-422X-5-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["17"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2008-04-22"],"dateOfCreation":["2008-03-06"],"dateOfRevision":["2009-11-18"],"electronicPublicationDate":["2008-01-24"],"firstPublicationDate":["2008-01-24"],"cprojectID":"PMC2263041"}}
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A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal. 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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqj6J9hGurOzZIYF","_score":1,"_source":{"id":["25132548"],"source":["MED"],"pmid":["25132548"],"pmcid":["PMC4240730"],"doi":["10.1038/nature13715"],"title":["Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies."],"authorString":["Wright KE, Hjerrild KA, Bartlett J, Douglas AD, Jin J, Brown RE, Illingworth JJ, Ashfield R, Clemmensen SB, de Jongh WA, Draper SJ, Higgins MK."],"authorList":[{"author":[{"fullName":["Wright KE"],"firstName":["Katherine E"],"lastName":["Wright"],"initials":["KE"],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK."]},{"fullName":["Hjerrild KA"],"firstName":["Kathryn A"],"lastName":["Hjerrild"],"initials":["KA"],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Bartlett J"],"firstName":["Jonathan"],"lastName":["Bartlett"],"initials":["J"],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK."]},{"fullName":["Douglas AD"],"firstName":["Alexander D"],"lastName":["Douglas"],"initials":["AD"],"authorId":[{"_":"0000-0002-5410-7562","$":{"type":"ORCID"}}],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Jin J"],"firstName":["Jing"],"lastName":["Jin"],"initials":["J"],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Brown RE"],"firstName":["Rebecca E"],"lastName":["Brown"],"initials":["RE"],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Illingworth JJ"],"firstName":["Joseph J"],"lastName":["Illingworth"],"initials":["JJ"],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Ashfield R"],"firstName":["Rebecca"],"lastName":["Ashfield"],"initials":["R"],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Clemmensen SB"],"firstName":["Stine B"],"lastName":["Clemmensen"],"initials":["SB"],"affiliation":["ExpreS2ion Biotechnologies, SCION-DTU Science Park, Agern Allé 1, DK-2970 Horsholm, Denmark."]},{"fullName":["de Jongh WA"],"firstName":["Willem A"],"lastName":["de Jongh"],"initials":["WA"],"affiliation":["ExpreS2ion Biotechnologies, SCION-DTU Science Park, Agern Allé 1, DK-2970 Horsholm, Denmark."]},{"fullName":["Draper SJ"],"firstName":["Simon J"],"lastName":["Draper"],"initials":["SJ"],"authorId":[{"_":"0000-0002-9415-1357","$":{"type":"ORCID"}}],"affiliation":["Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK."]},{"fullName":["Higgins MK"],"firstName":["Matthew K"],"lastName":["Higgins"],"initials":["MK"],"authorId":[{"_":"0000-0002-2870-1955","$":{"type":"ORCID"}}],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5410-7562","$":{"type":"ORCID"}},{"_":"0000-0002-9415-1357","$":{"type":"ORCID"}},{"_":"0000-0002-2870-1955","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["7527"],"volume":["515"],"journalIssueId":["2221095"],"dateOfPublication":["2014 Nov"],"monthOfPublication":["11"],"yearOfPublication":["2014"],"printPublicationDate":["2014-11-01"],"journal":[{"title":["Nature"],"ISOAbbreviation":["Nature"],"medlineAbbreviation":["Nature"],"NLMid":["0410462"],"ISSN":["0028-0836"],"ESSN":["1476-4687"]}]}],"pubYear":["2014"],"pageInfo":["427-430"],"abstractText":["Invasion of host erythrocytes is essential to the life cycle of Plasmodium parasites and development of the pathology of malaria. The stages of erythrocyte invasion, including initial contact, apical reorientation, junction formation, and active invagination, are directed by coordinated release of specialized apical organelles and their parasite protein contents. Among these proteins, and central to invasion by all species, are two parasite protein families, the reticulocyte-binding protein homologue (RH) and erythrocyte-binding like proteins, which mediate host-parasite interactions. RH5 from Plasmodium falciparum (PfRH5) is the only member of either family demonstrated to be necessary for erythrocyte invasion in all tested strains, through its interaction with the erythrocyte surface protein basigin (also known as CD147 and EMMPRIN). Antibodies targeting PfRH5 or basigin efficiently block parasite invasion in vitro, making PfRH5 an excellent vaccine candidate. Here we present crystal structures of PfRH5 in complex with basigin and two distinct inhibitory antibodies. PfRH5 adopts a novel fold in which two three-helical bundles come together in a kite-like architecture, presenting binding sites for basigin and inhibitory antibodies at one tip. This provides the first structural insight into erythrocyte binding by the Plasmodium RH protein family and identifies novel inhibitory epitopes to guide design of a new generation of vaccines against the blood-stage parasite."],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"grantId":["089455/2/09/z"],"agency":["Wellcome Trust"],"orderIn":["0"]},{"grantId":["101020"],"agency":["Wellcome Trust"],"orderIn":["0"]},{"grantId":["MR/K025554/1"],"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["G1000527"],"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["101020/Z/13/Z"],"agency":["Wellcome Trust"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Erythrocytes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Plasmodium falciparum"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Malaria"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PS"],"qualifierName":["parasitology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Protozoan Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Blocking"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antigens, Protozoan"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Epitopes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Crystallography, X-Ray"]},{"majorTopic_YN":["N"],"descriptorName":["Binding Sites"]},{"majorTopic_YN":["N"],"descriptorName":["Models, Molecular"]},{"majorTopic_YN":["N"],"descriptorName":["Antigens, CD147"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Host-Parasite Interactions"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Epitopes"],"registryNumber":["0"]},{"name":["Protozoan Proteins"],"registryNumber":["0"]},{"name":["Antibodies, Blocking"],"registryNumber":["0"]},{"name":["Antigens, Protozoan"],"registryNumber":["0"]},{"name":["Antigens, CD147"],"registryNumber":["136894-56-9"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4240730?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4240730"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/nature13715"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["20"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["PDB","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCompletion":["2015-01-13"],"dateOfCreation":["2014-11-21"],"dateOfRevision":["2015-05-23"],"electronicPublicationDate":["2014-08-17"],"firstPublicationDate":["2014-08-17"],"embargoDate":["2015-05-20"],"cprojectID":"PMC4240730"}}
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Intensive reproductive rhythm creates a negative energy balance in does : they are unable to consume enough feed (energy) for the nutritional requirements of foetus and lactation, and therefore lose most of their fat reserves. Furthermore, primiparous does also expend energy because they are still growing. Under intensive conditions, the 42-d reproductive rhythm (re-mating 11 days after parturition) is recommended. Under extensive conditions, the 18 or 25-d mating interval with 35 to 42-d weaning could be suitable. On small farms, natural mating is favoured; on large farms AI is commonly employed. The main advantage of AI is the all-in, all-out system. Hormonal (PMSG) treatment is used with AI to increase receptivity on d 11. Frequent and high level PMSG use can lead to higher anti-PMSG antibody rates. Lower level (max. 20 IU) and less frequent PMSG injection or non-hormonal alternative methods (short dam-litter separation, changing nursing method or lighting programs) are recommended for this reason."],"affiliation":["Department of Animal Medicine, Production and Health, University of Padova, Agripolis, Viale dell'Università, 16, 35020, Legnaro (PD), Italy ."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Energy balance","Hormonal Treatment","Biostimulation","Rabbit Does","Reproductive Rhythm","Farm Size"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4093116?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4093116"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=25049621"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=25049621&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.5713/ajas.2012.12015"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-07-22"],"dateOfCreation":["2014-07-22"],"dateOfRevision":["2014-07-28"],"electronicPublicationDate":["2012-05-01"],"firstPublicationDate":["2012-05-01"],"cprojectID":"PMC4093116"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoEhOUS2orL1IOd","_score":1,"_source":{"id":["25625872"],"source":["MED"],"pmid":["25625872"],"pmcid":["PMC4313657"],"doi":["10.3201/eid2102.141363"],"title":["Potential sexual transmission of Zika virus."],"authorString":["Musso D, Roche C, Robin E, Nhan T, Teissier A, Cao-Lormeau VM."],"authorList":[{"author":[{"fullName":["Musso D"],"firstName":["Didier"],"lastName":["Musso"],"initials":["D"]},{"fullName":["Roche C"],"firstName":["Claudine"],"lastName":["Roche"],"initials":["C"]},{"fullName":["Robin E"],"firstName":["Emilie"],"lastName":["Robin"],"initials":["E"]},{"fullName":["Nhan T"],"firstName":["Tuxuan"],"lastName":["Nhan"],"initials":["T"]},{"fullName":["Teissier A"],"firstName":["Anita"],"lastName":["Teissier"],"initials":["A"]},{"fullName":["Cao-Lormeau VM"],"firstName":["Van-Mai"],"lastName":["Cao-Lormeau"],"initials":["VM"]}]}],"journalInfo":[{"issue":["2"],"volume":["21"],"journalIssueId":["2246892"],"dateOfPublication":["2015 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2015"],"printPublicationDate":["2015-02-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2015"],"pageInfo":["359-361"],"abstractText":["In December 2013, during a Zika virus (ZIKV) outbreak in French Polynesia, a patient in Tahiti sought treatment for hematospermia, and ZIKV was isolated from his semen. ZIKV transmission by sexual intercourse has been previously suspected. This observation supports the possibility that ZIKV could be transmitted sexually."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Case Reports"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Sexually Transmitted Diseases, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Polynesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4313657?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4313657"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2102.141363"]}]}],"commentCorrectionList":[{"commentCorrection":[{"source":["MED"],"reference":["Emerg Infect Dis. 2015 Mar;21(3):552"],"type":["Erratum in"],"orderIn":["16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["32"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-10-15"],"dateOfCreation":["2015-01-28"],"dateOfRevision":["2016-02-03"],"firstPublicationDate":["2015-02-01"],"cprojectID":"PMC4313657"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoyhOUS2orL1IOk","_score":1,"_source":{"id":["26078854"],"source":["MED"],"pmid":["26078854"],"pmcid":["PMC4461419"],"doi":["10.1002/ece3.1481"],"title":["Separation in flowering time contributes to the maintenance of sympatric cryptic plant lineages."],"authorString":["Michalski SG, Durka W."],"authorList":[{"author":[{"fullName":["Michalski SG"],"firstName":["Stefan G"],"lastName":["Michalski"],"initials":["SG"],"affiliation":["Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ Theodor-Lieser-Strasse 4, Halle, D-06120, Germany."]},{"fullName":["Durka W"],"firstName":["Walter"],"lastName":["Durka"],"initials":["W"],"authorId":[{"_":"0000-0002-6611-2246","$":{"type":"ORCID"}}],"affiliation":["Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ Theodor-Lieser-Strasse 4, Halle, D-06120, Germany."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6611-2246","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["11"],"volume":["5"],"journalIssueId":["2295139"],"dateOfPublication":["2015 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2015"],"printPublicationDate":["2015-06-01"],"journal":[{"title":["Ecology and evolution"],"ISOAbbreviation":["Ecol Evol"],"medlineAbbreviation":["Ecol Evol"],"NLMid":["101566408"],"ESSN":["2045-7758"]}]}],"pubYear":["2015"],"pageInfo":["2172-2184"],"abstractText":["Sympatric cryptic lineages are a challenge for the understanding of species coexistence and lineage diversification as well as for management, conservation, and utilization of plant genetic resources. In higher plants studies providing insights into the mechanisms creating and maintaining sympatric cryptic lineages are rare. Here, using microsatellites and chloroplast sequence data, morphometric analyses, and phenological observations, we ask whether sympatrically coexisting lineages in the common wetland plant Juncus effusus are ecologically differentiated and reproductively isolated. Our results show two genetically highly differentiated, homoploid lineages within J. effusus that are morphologically cryptic and have similar preference for soil moisture content. However, flowering time differed significantly between the lineages contributing to reproductive isolation and the maintenance of these lineages. Furthermore, the later flowering lineage suffered less from predispersal seed predation by a Coleophora moth species. Still, we detected viable and reproducing hybrids between both lineages and the earlier flowering lineage and J. conglomeratus, a coexisting close relative. Flowering time differentiation between the lineages can be explained by neutral divergence alone and together with a lack of postzygotic isolation mechanisms; the sympatric coexistence of these lineages is most likely the result of an allopatric origin with secondary contact."],"affiliation":["Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ Theodor-Lieser-Strasse 4, Halle, D-06120, Germany."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Hybridization","Chloroplast DNA","Cryptic Species","Predispersal Seed Predation","Allochronic Isolation","Haplotype Sharing"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4461419?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4461419"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1002/ece3.1481"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-06-16"],"dateOfCreation":["2015-06-16"],"dateOfRevision":["2015-06-18"],"electronicPublicationDate":["2015-05-08"],"firstPublicationDate":["2015-05-08"],"cprojectID":"PMC4461419"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSTJ9hGurOzZIXl","_score":1,"_source":{"id":["22570700"],"source":["MED"],"pmid":["22570700"],"pmcid":["PMC3343050"],"doi":["10.1371/journal.pone.0036291"],"title":["First international external quality assessment study on molecular and serological methods for yellow fever diagnosis."],"authorString":["Domingo C, Escadafal C, Rumer L, Méndez JA, García P, Sall AA, Teichmann A, Donoso-Mantke O, Niedrig M."],"authorList":[{"author":[{"fullName":["Domingo C"],"firstName":["Cristina"],"lastName":["Domingo"],"initials":["C"]},{"fullName":["Escadafal C"],"firstName":["Camille"],"lastName":["Escadafal"],"initials":["C"]},{"fullName":["Rumer L"],"firstName":["Leonid"],"lastName":["Rumer"],"initials":["L"]},{"fullName":["Méndez JA"],"firstName":["Jairo A"],"lastName":["Méndez"],"initials":["JA"]},{"fullName":["García P"],"firstName":["Paquita"],"lastName":["García"],"initials":["P"]},{"fullName":["Sall AA"],"firstName":["Amadou A"],"lastName":["Sall"],"initials":["AA"]},{"fullName":["Teichmann A"],"firstName":["Anette"],"lastName":["Teichmann"],"initials":["A"]},{"fullName":["Donoso-Mantke O"],"firstName":["Oliver"],"lastName":["Donoso-Mantke"],"initials":["O"]},{"fullName":["Niedrig M"],"firstName":["Matthias"],"lastName":["Niedrig"],"initials":["M"]}]}],"journalInfo":[{"issue":["5"],"volume":["7"],"journalIssueId":["1929547"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2012"],"pageInfo":["e36291"],"abstractText":[" OBJECTIVE: We describe an external quality assurance (EQA) study designed to assess the efficiency and accurateness of molecular and serological methods used by expert laboratories performing YF diagnosis. STUDY DESIGN: For molecular diagnosis evaluation, a panel was prepared of 14 human plasma samples containing specific RNA of different YFV strains (YFV-17D, YFV South American strain [Brazil], YFV IvoryC1999 strain), and specificity samples containing other flaviviruses and negative controls. For the serological panel, 13 human plasma samples with anti-YFV-specific antibodies against different strains of YFV (YFV-17D strain, YFV IvoryC1999 strain, and YFV Brazilian strain), as well as specificity and negative controls, were included. RESULTS: Thirty-six laboratories from Europe, the Americas, Middle East, and Africa participated in these EQA activities. Only 16% of the analyses reported met all evaluation criteria with optimal performance. Serial dilutions of YFV-17D showed that in general the methodologies reported provided a suitable sensitivity. Failures were mainly due to the inability to detect wild-type strains or the presence of false positives. Performance in the serological diagnosis varied, mainly depending on the methodology used. Anti-YFV IgM detection was not performed in 16% of the reports using IIF or ELISA techniques, although it is preferable for the diagnosis of YFV acute infections. A good sensitivity profile was achieved in general; however, in the detection of IgM antibodies a lack of sensitivity of anti-YFV antibodies against the vaccine strain 17D was observed, and of the anti-YFV IgG antibodies against a West African strain. Neutralization assays showed a very good performance; however, the unexpected presence of false positives underlined the need of improving the running protocols. CONCLUSION: This EQA provides information on each laboratory's efficacy of RT-PCR and serological YFV diagnosis techniques. The results indicate the need for improving serological and molecular diagnosis techniques and provide a follow-up of the diagnostic profiles."],"affiliation":["Centre for Biological Security, Robert Koch Institute, Berlin, Germany. Domingo-CarrascoC@rki.de"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Vero Cells"]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Cercopithecus aethiops"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Yellow fever virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Serologic Tests"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]},{"abbreviation":["ST"],"qualifierName":["standards"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Diagnostic Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["N"]},{"abbreviation":["ST"],"qualifierName":["standards"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Load"]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ST"],"qualifierName":["standards"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Quality Assurance, Health Care"]},{"majorTopic_YN":["N"],"descriptorName":["Laboratory Proficiency Testing"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3343050?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3343050"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22570700&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22570700"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0036291"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["7"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-01-24"],"dateOfCreation":["2012-05-09"],"dateOfRevision":["2013-06-25"],"electronicPublicationDate":["2012-05-03"],"firstPublicationDate":["2012-05-03"],"cprojectID":"PMC3343050"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSQJ9hGurOzZIXg","_score":1,"_source":{"id":["21529382"],"source":["MED"],"pmid":["21529382"],"pmcid":["PMC3321797"],"doi":["10.3201/eid1705.110310"],"title":["Vector-borne infections."],"authorString":["Rosenberg R, Beard CB."],"authorList":[{"author":[{"fullName":["Rosenberg R"],"firstName":["Ronald"],"lastName":["Rosenberg"],"initials":["R"]},{"fullName":["Beard CB"],"firstName":["C Ben"],"lastName":["Beard"],"initials":["CB"]}]}],"journalInfo":[{"issue":["5"],"volume":["17"],"journalIssueId":["1827488"],"dateOfPublication":["2011 May"],"monthOfPublication":["5"],"yearOfPublication":["2011"],"printPublicationDate":["2011-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2011"],"pageInfo":["769-770"],"abstractText":["Infections with vector-borne pathogens are a major source of emerging diseases. The ability of vectors to bridge spatial and ecologic gaps between animals and humans increases opportunities for emergence. Small adaptations of a pathogen to a vector can have profound effects on the rate of transmission to humans."],"affiliation":["Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA. rrosenberg@cdc.gov"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, U.S. Gov't, P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Vectors"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3321797"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=21529382"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=21529382&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1705.110310"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["3"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2011-09-06"],"dateOfCreation":["2011-05-02"],"dateOfRevision":["2013-06-30"],"firstPublicationDate":["2011-05-01"],"cprojectID":"PMC3321797"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSUJ9hGurOzZIXm","_score":1,"_source":{"id":["22761884"],"source":["MED"],"pmid":["22761884"],"pmcid":["PMC3384601"],"doi":["10.1371/journal.pone.0039741"],"title":["Development of AAVLP(HPV16/31L2) particles as broadly protective HPV vaccine candidate."],"authorString":["Nieto K, Weghofer M, Sehr P, Ritter M, Sedlmeier S, Karanam B, Seitz H, Müller M, Kellner M, Hörer M, Michaelis U, Roden RB, Gissmann L, Kleinschmidt JA."],"authorList":[{"author":[{"fullName":["Nieto K"],"firstName":["Karen"],"lastName":["Nieto"],"initials":["K"]},{"fullName":["Weghofer M"],"firstName":["Margit"],"lastName":["Weghofer"],"initials":["M"]},{"fullName":["Sehr P"],"firstName":["Peter"],"lastName":["Sehr"],"initials":["P"]},{"fullName":["Ritter M"],"firstName":["Mirko"],"lastName":["Ritter"],"initials":["M"]},{"fullName":["Sedlmeier S"],"firstName":["Sebastian"],"lastName":["Sedlmeier"],"initials":["S"]},{"fullName":["Karanam B"],"firstName":["Balasubramanyam"],"lastName":["Karanam"],"initials":["B"]},{"fullName":["Seitz H"],"firstName":["Hanna"],"lastName":["Seitz"],"initials":["H"]},{"fullName":["Müller M"],"firstName":["Martin"],"lastName":["Müller"],"initials":["M"]},{"fullName":["Kellner M"],"firstName":["Markus"],"lastName":["Kellner"],"initials":["M"]},{"fullName":["Hörer M"],"firstName":["Markus"],"lastName":["Hörer"],"initials":["M"]},{"fullName":["Michaelis U"],"firstName":["Uwe"],"lastName":["Michaelis"],"initials":["U"]},{"fullName":["Roden RB"],"firstName":["Richard B S"],"lastName":["Roden"],"initials":["RB"]},{"fullName":["Gissmann L"],"firstName":["Lutz"],"lastName":["Gissmann"],"initials":["L"]},{"fullName":["Kleinschmidt JA"],"firstName":["Jürgen A"],"lastName":["Kleinschmidt"],"initials":["JA"]}]}],"journalInfo":[{"issue":["6"],"volume":["7"],"journalIssueId":["1937981"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2012"],"pageInfo":["e39741"],"abstractText":["The human papillomavirus (HPV) minor capsid protein L2 is a promising candidate for a broadly protective HPV vaccine yet the titers obtained in most experimental systems are rather low. Here we examine the potential of empty AAV2 particles (AAVLPs), assembled from VP3 alone, for display of L2 epitopes to enhance their immunogenicity. Insertion of a neutralizing epitope (amino acids 17-36) from L2 of HPV16 and HPV31 into VP3 at positions 587 and 453, respectively, permitted assembly into empty AAV particles (AAVLP(HPV16/31L2)). Intramuscularly vaccination of mice and rabbits with AAVLP(HPV16/31L2)s in montanide adjuvant, induced high titers of HPV16 L2 antibodies as measured by ELISA. Sera obtained from animals vaccinated with the AAVLP(HPV16/31L2)s neutralized infections with several HPV types in a pseudovirion infection assay. Lyophilized AAVLP(HPV16/31L2) particles retained their immunogenicity upon reconstitution. Interestingly, vaccination of animals that were pre-immunized with AAV2--simulating the high prevalence of AAV2 antibodies in the population--even increased cross neutralization against HPV31, 45 and 58 types. Finally, passive transfer of rabbit antisera directed against AAVLP(HPV16/31L2)s protected naïve mice from vaginal challenge with HPV16 pseudovirions. In conclusion, AAVLP(HPV16/31L2) particles have the potential as a broadly protective vaccine candidate regardless of prior exposure to AAV."],"affiliation":["Research Program Infection and Cancer, German Cancer Research Center, Heidelberg, Germany. k.nieto@dkfz.de"],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["RC2 CA148499"],"agency":["NCI NIH HHS"],"acronym":["CA"],"orderIn":["0"]},{"grantId":["P50 CA098252"],"agency":["NCI NIH HHS"],"acronym":["CA"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Mice, Inbred BALB C"]},{"majorTopic_YN":["N"],"descriptorName":["Mice, Inbred C57BL"]},{"majorTopic_YN":["N"],"descriptorName":["Rabbits"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Papillomaviridae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Virion"]},{"majorTopic_YN":["N"],"descriptorName":["Viral Vaccines"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AD"],"qualifierName":["administration & dosage"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Adjuvants, Immunologic"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AD"],"qualifierName":["administration & dosage"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Enzyme-Linked Immunosorbent Assay"]}]}],"chemicalList":[{"chemical":[{"name":["Viral Vaccines"],"registryNumber":["0"]},{"name":["Adjuvants, Immunologic"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3384601?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3384601"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22761884&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22761884"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0039741"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["22"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-01-04"],"dateOfCreation":["2012-07-04"],"dateOfRevision":["2013-07-12"],"electronicPublicationDate":["2012-06-27"],"firstPublicationDate":["2012-06-27"],"cprojectID":"PMC3384601"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSXhOUS2orL1INy","_score":1,"_source":{"id":["19014417"],"source":["MED"],"pmid":["19014417"],"pmcid":["PMC2590599"],"doi":["10.1186/1756-8935-1-8"],"title":["Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes."],"authorString":["Lepikhov K, Zakhartchenko V, Hao R, Yang F, Wrenzycki C, Niemann H, Wolf E, Walter J."],"authorList":[{"author":[{"fullName":["Lepikhov K"],"firstName":["Konstantin"],"lastName":["Lepikhov"],"initials":["K"]},{"fullName":["Zakhartchenko V"],"firstName":["Valeri"],"lastName":["Zakhartchenko"],"initials":["V"],"authorId":[{"_":"0000-0002-5974-9759","$":{"type":"ORCID"}}]},{"fullName":["Hao R"],"firstName":["Ru"],"lastName":["Hao"],"initials":["R"]},{"fullName":["Yang F"],"firstName":["Feikun"],"lastName":["Yang"],"initials":["F"]},{"fullName":["Wrenzycki C"],"firstName":["Christine"],"lastName":["Wrenzycki"],"initials":["C"]},{"fullName":["Niemann H"],"firstName":["Heiner"],"lastName":["Niemann"],"initials":["H"]},{"fullName":["Wolf E"],"firstName":["Eckhard"],"lastName":["Wolf"],"initials":["E"],"authorId":[{"_":"0000-0002-0430-9510","$":{"type":"ORCID"}}]},{"fullName":["Walter J"],"firstName":["Joern"],"lastName":["Walter"],"initials":["J"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-5974-9759","$":{"type":"ORCID"}},{"_":"0000-0002-0430-9510","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["1"],"journalIssueId":["1579535"],"dateOfPublication":["2008 "],"monthOfPublication":["0"],"yearOfPublication":["2008"],"printPublicationDate":["2008-01-01"],"journal":[{"title":["Epigenetics & chromatin"],"ISOAbbreviation":["Epigenetics Chromatin"],"medlineAbbreviation":["Epigenetics Chromatin"],"NLMid":["101471619"],"ESSN":["1756-8935"]}]}],"pubYear":["2008"],"pageInfo":["8"],"abstractText":[" BACKGROUND: In mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of the paternal (sperm-derived) chromosomes prior to DNA replication in zygotes. Such active DNA demethylation in the zygote has been documented for several mammalian species, including mouse, rat, pig, human and cow, but questioned to occur in rabbit. RESULTS: When comparing immunohistochemical patterns of antibodies against 5-methyl-cytosine, H3K4me3 and H3K9me2 modifications we observe similar pronuclear distribution and dynamics in mouse, bovine and rabbit zygotes. In rabbit DNA demethylation of the paternal chromosomes occurs at slightly advanced pronuclear stages. We also show that the rabbit oocyte rapidly demethylates DNA of donor fibroblast after nuclear transfer. CONCLUSION: Our data reveal that major events of epigenetic reprogramming during pronuclear maturation, including mechanisms of active DNA demethylation, are apparently conserved among mammalian species."],"affiliation":["University of Saarland, Natural Sciences - Technical Faculty III, Biological Sciences, Genetics/Epigenetics, 66123 Saarbrücken, Germany. k.lepikhov@mx.uni-saarland.de"],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2590599?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2590599"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=19014417&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=19014417"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1756-8935-1-8"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["18"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2010-06-09"],"dateOfCreation":["2008-12-01"],"electronicPublicationDate":["2008-11-03"],"firstPublicationDate":["2008-11-03"],"cprojectID":"PMC2590599"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqUh_8VqgcuJwvf6","_score":1,"_source":{"id":["22953014"],"source":["MED"],"pmid":["22953014"],"pmcid":["PMC3429392"],"doi":["10.1371/journal.pntd.0001792"],"title":["Oral susceptibility of Singapore Aedes (Stegomyia) aegypti (Linnaeus) to Zika virus."],"authorString":["Li MI, Wong PS, Ng LC, Tan CH."],"authorList":[{"author":[{"fullName":["Li MI"],"firstName":["MeiZhi Irene"],"lastName":["Li"],"initials":["MI"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Wong PS"],"firstName":["Pei Sze Jeslyn"],"lastName":["Wong"],"initials":["PS"]},{"fullName":["Ng LC"],"firstName":["Lee Ching"],"lastName":["Ng"],"initials":["LC"]},{"fullName":["Tan CH"],"firstName":["Cheong Huat"],"lastName":["Tan"],"initials":["CH"]}]}],"journalInfo":[{"issue":["8"],"volume":["6"],"journalIssueId":["1992261"],"dateOfPublication":["2012 "],"monthOfPublication":["0"],"yearOfPublication":["2012"],"printPublicationDate":["2012-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2012"],"pageInfo":["e1792"],"abstractText":["Zika virus (ZIKV) is a little known flavivirus that caused a major outbreak in 2007, in the South-western Pacific Island of Yap. It causes dengue-like syndromes but with milder symptoms. In Africa, where it was first isolated, ZIKV is mainly transmitted by sylvatic Aedes mosquitoes. The virus has also been isolated from Ae. aegypti and it is considered to be the vector involved in the urban transmission of the virus. Transmission of the virus by an African strain of Ae. aegypti has also been demonstrated under laboratory conditions. The aim of the present study is to describe the oral susceptibility of a Singapore strain of Ae. aegypti to ZIKV, under conditions that simulate local climate.To assess the receptivity of Singapore's Ae. aegypti to the virus, we orally exposed a local mosquito strain to a Ugandan strain of ZIKV. Upon exposure, fully engorged mosquitoes were maintained in an environmental chamber set at 29 °C and 70-75% RH. Eight mosquitoes were then sampled daily from day 1 to day 7, and subsequently on days 10 and 14 post exposure (pe). The virus titer of the midgut and salivary glands of each mosquito were determined using a tissue culture infectious dose(50) (TCID(50)) assay. High midgut infection and salivary gland dissemination rates were observed. By day 5 after the infectious blood meal, ZIKV was found in the salivary glands of more than half of the mosquitoes tested (62%); and by day 10, all mosquitoes were potentially infective.This study showed that Singapore's urban Ae. aegypti are susceptible and are potentially capable of transmitting ZIKV. The virus could be established in Singapore should it be introduced. Nevertheless, Singapore's current dengue control strategy is applicable to control ZIKV."],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Digestive System"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Singapore"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3429392?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3429392"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=22953014"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=22953014&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0001792"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["11"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-01-03"],"dateOfCreation":["2012-09-06"],"dateOfRevision":["2016-02-05"],"electronicPublicationDate":["2012-08-28"],"firstPublicationDate":["2012-08-28"],"cprojectID":"PMC3429392"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSfhOUS2orL1IN9","_score":1,"_source":{"id":["24603874"],"source":["MED"],"pmid":["24603874"],"pmcid":["PMC3945292"],"doi":["10.1371/journal.pntd.0002730"],"title":["Rapid molecular assays for the detection of yellow fever virus in low-resource settings."],"authorString":["Escadafal C, Faye O, Sall AA, Faye O, Weidmann M, Strohmeier O, von Stetten F, Drexler J, Eberhard M, Niedrig M, Patel P."],"authorList":[{"author":[{"fullName":["Escadafal C"],"firstName":["Camille"],"lastName":["Escadafal"],"initials":["C"],"affiliation":["Centre for Biological Threats and Special Pathogens 1, Robert Koch Institute, Berlin, Germany."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Department of Arboviruses, Institute Pasteur of Dakar, Dakar, Senegal."]},{"fullName":["Sall AA"],"firstName":["Amadou Alpha"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Department of Arboviruses, Institute Pasteur of Dakar, Dakar, Senegal."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Department of Arboviruses, Institute Pasteur of Dakar, Dakar, Senegal."]},{"fullName":["Weidmann M"],"firstName":["Manfred"],"lastName":["Weidmann"],"initials":["M"],"authorId":[{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}],"affiliation":["Department of Virology, University Medical Centre, Göttingen, Germany; Institute of Aquaculture, University of Stirling, Stirling, United Kingdom."]},{"fullName":["Strohmeier O"],"firstName":["Oliver"],"lastName":["Strohmeier"],"initials":["O"],"affiliation":["Laboratory for MEMS Applications, Department of Microsystems Engineering - IMTEK, University of Freiburg, Freiburg, Germany; HSG-IMIT - Institut für Mikro- und Informationstechnik, Freiburg, Germany."]},{"fullName":["von Stetten F"],"firstName":["Felix"],"lastName":["von Stetten"],"initials":["F"],"affiliation":["Laboratory for MEMS Applications, Department of Microsystems Engineering - IMTEK, University of Freiburg, Freiburg, Germany; HSG-IMIT - Institut für Mikro- und Informationstechnik, Freiburg, Germany."]},{"fullName":["Drexler J"],"firstName":["Josef"],"lastName":["Drexler"],"initials":["J"],"affiliation":["QIAGEN Lake Constance GmbH, Stockach, Germany."]},{"fullName":["Eberhard M"],"firstName":["Michael"],"lastName":["Eberhard"],"initials":["M"],"affiliation":["QIAGEN Lake Constance GmbH, Stockach, Germany."]},{"fullName":["Niedrig M"],"firstName":["Matthias"],"lastName":["Niedrig"],"initials":["M"],"affiliation":["Centre for Biological Threats and Special Pathogens 1, Robert Koch Institute, Berlin, Germany."]},{"fullName":["Patel P"],"firstName":["Pranav"],"lastName":["Patel"],"initials":["P"],"affiliation":["Centre for Biological Threats and Special Pathogens 1, Robert Koch Institute, Berlin, Germany."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["8"],"journalIssueId":["2137004"],"dateOfPublication":["2014 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2014"],"printPublicationDate":["2014-03-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2014"],"pageInfo":["e2730"],"abstractText":["BACKGROUND: Yellow fever (YF) is an acute viral hemorrhagic disease transmitted by Aedes mosquitoes. The causative agent, the yellow fever virus (YFV), is found in tropical and subtropical areas of South America and Africa. Although a vaccine is available since the 1930s, YF still causes thousands of deaths and several outbreaks have recently occurred in Africa. Therefore, rapid and reliable diagnostic methods easy to perform in low-resources settings could have a major impact on early detection of outbreaks and implementation of appropriate response strategies such as vaccination and/or vector control. METHODOLOGY: The aim of this study was to develop a YFV nucleic acid detection method applicable in outbreak investigations and surveillance studies in low-resource and field settings. The method should be simple, robust, rapid and reliable. Therefore, we adopted an isothermal approach and developed a recombinase polymerase amplification (RPA) assay which can be performed with a small portable instrument and easy-to-use lyophilized reagents. The assay was developed in three different formats (real-time with or without microfluidic semi-automated system and lateral-flow assay) to evaluate their application for different purposes. Analytical specificity and sensitivity were evaluated with a wide panel of viruses and serial dilutions of YFV RNA. Mosquito pools and spiked human plasma samples were also tested for assay validation. Finally, real-time RPA in portable format was tested under field conditions in Senegal. CONCLUSION/SIGNIFICANCE: The assay was able to detect 20 different YFV strains and demonstrated no cross-reactions with closely related viruses. The RPA assay proved to be a robust, portable method with a low detection limit (<21 genome equivalent copies per reaction) and rapid processing time (<20 min). Results from real-time RPA field testing were comparable to results obtained in the laboratory, thus confirming our method is suitable for YFV detection in low-resource settings."],"affiliation":["Centre for Biological Threats and Special Pathogens 1, Robert Koch Institute, Berlin, Germany."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow fever virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Diagnostic Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Nucleic Acid Amplification Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Developing Countries"]},{"majorTopic_YN":["N"],"descriptorName":["Senegal"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3945292?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3945292"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24603874&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24603874"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0002730"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["8"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-10-16"],"dateOfCreation":["2014-03-07"],"electronicPublicationDate":["2014-03-06"],"firstPublicationDate":["2014-03-06"],"cprojectID":"PMC3945292"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSPJ9hGurOzZIXf","_score":1,"_source":{"id":["18275613"],"source":["MED"],"pmid":["18275613"],"pmcid":["PMC2275255"],"doi":["10.1186/1743-422x-5-27"],"title":["Constancy and diversity in the flavivirus fusion peptide."],"authorString":["Seligman SJ."],"authorList":[{"author":[{"fullName":["Seligman SJ"],"firstName":["Stephen J"],"lastName":["Seligman"],"initials":["SJ"]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["1508293"],"dateOfPublication":["2008 "],"monthOfPublication":["0"],"yearOfPublication":["2008"],"printPublicationDate":["2008-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2008"],"pageInfo":["27"],"abstractText":[" BACKGROUND: Flaviviruses include the mosquito-borne dengue, Japanese encephalitis, yellow fever and West Nile and the tick-borne encephalitis viruses. They are responsible for considerable world-wide morbidity and mortality. Viral entry is mediated by a conserved fusion peptide containing 16 amino acids located in domain II of the envelope protein E. Highly orchestrated conformational changes initiated by exposure to acidic pH accompany the fusion process and are important factors limiting amino acid changes in the fusion peptide that still permit fusion with host cell membranes in both arthropod and vertebrate hosts. The cell-fusing related agents, growing only in mosquitoes or insect cell lines, possess a different homologous peptide. RESULTS: Analysis of 46 named flaviviruses deposited in the Entrez Nucleotides database extended the constancy in the canonical fusion peptide sequences of mosquito-borne, tick-borne and viruses with no known vector to include more recently-sequenced viruses. The mosquito-borne signature amino acid, G104, was also found in flaviviruses with no known vector and with the cell-fusion related viruses. Despite the constancy in the canonical sequences in pathogenic flaviviruses, mutations were surprisingly frequent with a 27% prevalence of nonsynonymous mutations in yellow fever virus fusion peptide sequences, and 0 to 7.4% prevalence in the others. Six of seven yellow fever patients whose virus had fusion peptide mutations died. In the cell-fusing related agents, not enough sequences have been deposited to estimate reliably the prevalence of fusion peptide mutations. However, the canonical sequences homologous to the fusion peptide and the pattern of disulfide linkages in protein E differed significantly from the other flaviviruses. CONCLUSION: The constancy of the canonical fusion peptide sequences in the arthropod-borne flaviviruses contrasts with the high prevalence of mutations in most individual viruses. The discrepancy may be the result of a survival advantage accompanying sequence diversity (quasispecies) involving the fusion peptide. Limited clinical data with yellow fever virus suggest that the presence of fusion peptide mutants is not associated with a decreased case fatality rate. The cell-fusing related agents may have substantial differences from other flaviviruses in their mechanism of viral entry into the host cell."],"affiliation":["Department of Microbiology and Immunology, New York Medical College, Valhalla, New York, USA. stephen_seligman@nymc.edu"],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["U54-AI057158"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Yellow fever virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Peptides"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Envelope Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cell Fusion"]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acid Substitution"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Alignment"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acid Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Conserved Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Homology, Amino Acid"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Databases, Genetic"]}]}],"chemicalList":[{"chemical":[{"name":["Peptides"],"registryNumber":["0"]},{"name":["Viral Envelope Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2275255?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2275255"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=18275613"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=18275613&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1743-422X-5-27"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["11"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2008-04-22"],"dateOfCreation":["2008-03-26"],"dateOfRevision":["2009-11-18"],"electronicPublicationDate":["2008-02-14"],"firstPublicationDate":["2008-02-14"],"cprojectID":"PMC2275255"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSahOUS2orL1IN1","_score":1,"_source":{"id":["18505720"],"source":["MED"],"pmid":["18505720"],"pmcid":["PMC2475551"],"doi":["10.1098/rspb.2008.0294"],"title":["Temporal trends in the discovery of human viruses."],"authorString":["Woolhouse ME, Howey R, Gaunt E, Reilly L, Chase-Topping M, Savill N."],"authorList":[{"author":[{"fullName":["Woolhouse ME"],"firstName":["Mark E J"],"lastName":["Woolhouse"],"initials":["ME"]},{"fullName":["Howey R"],"firstName":["Richard"],"lastName":["Howey"],"initials":["R"]},{"fullName":["Gaunt E"],"firstName":["Eleanor"],"lastName":["Gaunt"],"initials":["E"]},{"fullName":["Reilly L"],"firstName":["Liam"],"lastName":["Reilly"],"initials":["L"]},{"fullName":["Chase-Topping M"],"firstName":["Margo"],"lastName":["Chase-Topping"],"initials":["M"]},{"fullName":["Savill N"],"firstName":["Nick"],"lastName":["Savill"],"initials":["N"]}]}],"journalInfo":[{"issue":["1647"],"volume":["275"],"journalIssueId":["1545974"],"dateOfPublication":["2008 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2008"],"printPublicationDate":["2008-09-01"],"journal":[{"title":["Proceedings. Biological sciences / The Royal Society"],"ISOAbbreviation":["Proc. Biol. Sci."],"medlineAbbreviation":["Proc Biol Sci"],"NLMid":["101245157"],"ISSN":["0962-8452"],"ESSN":["1471-2954"]}]}],"pubYear":["2008"],"pageInfo":["2111-2115"],"abstractText":["On average, more than two new species of human virus are reported every year. We constructed the cumulative species discovery curve for human viruses going back to 1901. We fitted a statistical model to these data; the shape of the curve strongly suggests that the process of virus discovery is far from complete. We generated a 95% credible interval for the pool of as yet undiscovered virus species of 38-562. We extrapolated the curve and generated an estimate of 10-40 new species to be discovered by 2020. Although we cannot predict the level of health threat that these new viruses will present, we conclude that novel virus species must be anticipated in public health planning. More systematic virus discovery programmes, covering both humans and potential animal reservoirs of human viruses, should be considered."],"affiliation":["Centre for Infectious Diseases, Ashworth Laboratories, Kings Buildings, University of Edinburgh, Edinburgh EH9 3JT, UK. mark.woolhouse@ed.ac.uk"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Historical Article","Journal Article","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"agency":["Wellcome Trust"],"orderIn":["0"]},{"agency":["Biotechnology and Biological Sciences Research Council"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Viruses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zoonoses"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Virus Diseases"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Linear Models"]},{"majorTopic_YN":["N"],"descriptorName":["Probability"]},{"majorTopic_YN":["N"],"descriptorName":["Regression Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Public Health"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["HI"],"qualifierName":["history"],"majorTopic_YN":["N"]},{"abbreviation":["TD"],"qualifierName":["trends"],"majorTopic_YN":["Y"]},{"abbreviation":["SN"],"qualifierName":["statistics & numerical data"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["History, 20th Century"]},{"majorTopic_YN":["N"],"descriptorName":["History, 21st Century"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2475551?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2475551"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=18505720&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=18505720"]},{"availability":["Free after 12 months"],"availabilityCode":["F12"],"documentStyle":["abs"],"site":["www.journals.royalsoc.ac.uk"],"url":["http://www.journals.royalsoc.ac.uk/openurl.asp?genre=article&issn=0962-8452&volume=275&issue=1647&spage=2111"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1098/rspb.2008.0294"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["23"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2008-10-10"],"dateOfCreation":["2008-08-01"],"dateOfRevision":["2013-06-05"],"firstPublicationDate":["2008-09-01"],"cprojectID":"PMC2475551"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSOJ9hGurOzZIXd","_score":1,"_source":{"id":["3545807"],"source":["MED"],"pmid":["3545807"],"pmcid":["PMC1474299"],"title":["Occurrence of by-products of strong oxidants reacting with drinking water contaminants--scope of the problem."],"authorString":["Rice RG, Gomez-Taylor M."],"authorList":[{"author":[{"fullName":["Rice RG"],"lastName":["Rice"],"initials":["RG"]},{"fullName":["Gomez-Taylor M"],"lastName":["Gomez-Taylor"],"initials":["M"]}]}],"journalInfo":[{"volume":["69"],"journalIssueId":["813458"],"dateOfPublication":["1986 Nov"],"monthOfPublication":["11"],"yearOfPublication":["1986"],"printPublicationDate":["1986-11-01"],"journal":[{"title":["Environmental health perspectives"],"ISOAbbreviation":["Environ. 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The presence of chloronitrile compounds in drinking waters is indicated to arise from reactions of chlorine or chloramine to amine/amide functions in amino acids or proteinaceous materials, followed by dehydrohalogenation. These nitriles could hydrolyze to produce the corresponding chloroacetic acids. It is concluded that to minimize the presence of oxidation by-products in drinking waters, the concentrations of oxidizable organic/inorganic impurities should be lowered before any oxidizing agent is added."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["Y"],"descriptorName":["Chlorine Compounds"]},{"majorTopic_YN":["N"],"descriptorName":["Oxides"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chlorine"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ozone"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chloramines"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disinfectants"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sewage"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Water Supply"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AN"],"qualifierName":["analysis"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Oxidation-Reduction"]},{"majorTopic_YN":["N"],"descriptorName":["Chemistry"]},{"majorTopic_YN":["N"],"descriptorName":["Chemical Phenomena"]}]}],"chemicalList":[{"chemical":[{"name":["chlorine dioxide"],"registryNumber":["10049-04-4"]},{"name":["chloramine"],"registryNumber":["10599-90-3"]},{"name":["Ozone"],"registryNumber":["10028-15-6"]},{"name":["Chloramines"],"registryNumber":["0"]},{"name":["Oxides"],"registryNumber":["0"]},{"name":["Disinfectants"],"registryNumber":["0"]},{"name":["Chlorine"],"registryNumber":["7782-50-5"]},{"name":["Chlorine Compounds"],"registryNumber":["0"]},{"name":["Sewage"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1474299?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1474299"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=3545807&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=3545807"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["7"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["1987-04-20"],"dateOfCreation":["1987-04-20"],"dateOfRevision":["2009-11-18"],"firstPublicationDate":["1986-11-01"],"cprojectID":"PMC1474299"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsGn_8VqgcuJwvkk","_score":1,"_source":{"id":["27541001"],"source":["MED"],"pmid":["27541001"],"pmcid":["PMC4991799"],"doi":["10.1371/journal.pntd.0004931"],"title":["Working with Zika and Usutu Viruses In Vitro."],"authorString":["Barr KL, Anderson BD, Prakoso D, Long MT."],"authorList":[{"author":[{"fullName":["Barr KL"],"firstName":["Kelli L"],"lastName":["Barr"],"initials":["KL"],"affiliation":["Department of Infectious Diseases & Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Anderson BD"],"firstName":["Benjamin D"],"lastName":["Anderson"],"initials":["BD"],"affiliation":["Division of Infectious Disease, School of Medicine and Global Health Institute, Duke University, Durham, North Carolina, United States of America."]},{"fullName":["Prakoso D"],"firstName":["Dhani"],"lastName":["Prakoso"],"initials":["D"],"affiliation":["Department of Infectious Diseases & Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America."]},{"fullName":["Long MT"],"firstName":["Maureen T"],"lastName":["Long"],"initials":["MT"],"affiliation":["Department of Infectious Diseases & Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004931"],"abstractText":["Usutu (USUV) and Zika (ZIKV) viruses are emerging arboviruses of significant medical and veterinary importance. These viruses have not been studied as well as other medically important arboviruses such as West Nile (WNV), dengue (DENV), or chikungunya (CHIKV) viruses. As such, information regarding the behavior of ZIKV and USUV viruses in the laboratory is dated. Usutu virus re-emerged in Austria in 2001 and has since spread throughout the European and Asian continents causing significant mortality among birds. Zika virus has recently appeared in the Western Hemisphere and has exhibited high rates of birth defects and sexual transmission. Information about the characteristics of USUV and ZIKV viruses are needed to better understand the transmission, dispersal, and adaptation of these viruses in new environments. Since their initial characterization in the middle of last century, technologies and reagents have been developed that could enhance our abilities to study these pathogens. Currently, standard laboratory methods for these viruses are limited to 2-3 cell lines and many assays take several days to generate meaningful data. The goal of this study was to characterize these viruses in cells from multiple diverse species. Cell lines from 17 species were permissive to both ZIKV and USUV. These viruses were able to replicate to significant titers in most of the cell lines tested. Moreover, cytopathic effects were observed in 8 of the cell lines tested. These data indicate that a variety of cell lines can be used to study ZIKV and USUV infection and may provide an updated foundation for the study of host-pathogen interactions, model development, and the development of therapeutics."],"affiliation":["Department of Infectious Diseases & Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991799?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4991799"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004931"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-20"],"electronicPublicationDate":["2016-08-19"],"firstPublicationDate":["2016-08-19"],"cprojectID":"PMC4991799"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqUh_8VqgcuJwvf7","_score":1,"_source":{"id":["24148652"],"source":["MED"],"pmid":["24148652"],"pmcid":["PMC4016539"],"doi":["10.1186/1743-422x-10-311"],"title":["Quantitative real-time PCR detection of Zika virus and evaluation with field-caught mosquitoes."],"authorString":["Faye O, Faye O, Diallo D, Diallo M, Weidmann M, Sall AA."],"authorList":[{"author":[{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"]},{"fullName":["Diallo D"],"firstName":["Diawo"],"lastName":["Diallo"],"initials":["D"]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"]},{"fullName":["Weidmann M"],"firstName":["Manfred"],"lastName":["Weidmann"],"initials":["M"],"authorId":[{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}]},{"fullName":["Sall AA"],"firstName":["Amadou Alpha"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Unité des Arbovirus et virus de fièvres hémorragiques, Institut Pasteur Dakar, 36, Avenue Pasteur, BP 220 Dakar, Senegal. asall@pasteur.sn."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2002717"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2013"],"pageInfo":["311"],"abstractText":["Zika virus (ZIKV), a mosquito borne flavivirus is a pathogen affecting humans in Asia and Africa. ZIKV infection diagnosis relies on serology-which is challenging due to cross-reactions with other flaviviruses and/or absence or low titer of IgM and IgG antibodies at early phase of infection- virus isolation, which is labor intensive, time consuming and requires appropriate containment. Therefore, real-time RT-PCR (rRT-PCR) is an appealing option as a rapid, sensitive and specific method for detection of ZIKV in the early stage of infection. So far, only one rRT-PCR assay has been described in the context of the outbreak in Micronesia in 2007. In this study, we described a one step rRT-PCR for ZIKV which can detect a wider genetic diversity of ZIKV isolates from Asia and Africa.The NS5 protein coding regions of African ZIKV isolates were sequenced and aligned with representative flaviviruses sequences from GenBank to design primers and probe from conserved regions. The analytical sensitivity of the assay was evaluated to be 32 genome-equivalents and 0.05 plaque forming unit (pfu). The assay was shown to detect 37 ZIKV isolates covering a wide geographic in Africa and Asia over 36 years but none of the 31 other flaviviruses tested showing high analytical specificity. The rRT-PCR could be performed in less than 3 hours. This method was used successfully to detect ZIKV strains from field-caught mosquitoes.We have developed a rapid, sensitive and specific rRT-PCR for detection of ZIKV. This assay is a useful tool for detection of ZIKV infection in regions where a number of other clinically indistinguishable arboviruses like dengue or chikungunya co-circulate. Further studies are needed to validate this assay in clinical positive samples collected during acute ZIKV infection."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Evaluation Studies","Journal Article","Research Support, Non-U.S. Gov't","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["5R01A1069145"],"agency":["PHS HHS"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Culicidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Diagnostic Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Time Factors"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4016539?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4016539"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24148652"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24148652&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/1743-422X-10-311"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["15"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," refseq"," "]}],"dateOfCompletion":["2014-07-14"],"dateOfCreation":["2014-01-16"],"dateOfRevision":["2016-02-04"],"electronicPublicationDate":["2013-10-22"],"firstPublicationDate":["2013-10-22"],"cprojectID":"PMC4016539"}}
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It includes a set of clinically relevant infectious agents such as dengue, West Nile, yellow fever, and Japanese encephalitis viruses. Although these pathogens have been studied extensively, safe and efficient vaccines lack for the majority of the flaviviruses.We have assembled a database that combines antigenic data of flaviviruses, specialized analysis tools, and workflows for automated complex analyses focusing on applications in immunology and vaccinology. FLAVIdB contains 12,858 entries of flavivirus antigen sequences, 184 verified T-cell epitopes, 201 verified B-cell epitopes, and 4 representative molecular structures of the dengue virus envelope protein. FLAVIdB was assembled by collection, annotation, and integration of data from GenBank, GenPept, UniProt, IEDB, and PDB. The data were subject to extensive quality control (redundancy elimination, error detection, and vocabulary consolidation). Further annotation of selected functionally relevant features was performed by organizing information extracted from the literature. The database was incorporated into a web-accessible data mining system, combining specialized data analysis tools for integrated analysis of relevant data categories (protein sequences, macromolecular structures, and immune epitopes). The data mining system includes tools for variability and conservation analysis, T-cell epitope prediction, and characterization of neutralizing components of B-cell epitopes. FLAVIdB is accessible at cvc.dfci.harvard.edu/flavi/FLAVIdB represents a new generation of databases in which data and tools are integrated into a data mining infrastructures specifically designed to aid rational vaccine design by discovery of vaccine targets."],"affiliation":["Cancer Vaccine Center, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA ; Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["U01 AI090043"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4276368?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4276368"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2014-12-29"],"dateOfRevision":["2015-01-13"],"firstPublicationDate":["2011-01-01"],"cprojectID":"PMC4276368"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqnKhOUS2orL1IOV","_score":1,"_source":{"id":["24843290"],"source":["MED"],"pmid":["24843290"],"pmcid":["PMC4023339"],"doi":["10.3897/phytokeys.36.6784"],"title":["Crataegus ×ninae-celottiae and C. ×cogswellii (Rosaceae, Maleae), two spontaneously formed intersectional nothospecies."],"authorString":["Christensen KI, Zarrei M, Kuzmina M, Talent N, Lin C, Dickinson TA."],"authorList":[{"author":[{"fullName":["Christensen KI"],"firstName":["Knud Ib"],"lastName":["Christensen"],"initials":["KI"],"affiliation":["Assoc. Prof. M.Sc. Ph.D. Knud Ib Christensen (born 13 October 1955, deceased 16 January 2012), formerly at the Botanical Garden, Natural History Museum of Denmark, University of Copenhagen."]},{"fullName":["Zarrei M"],"firstName":["Mehdi"],"lastName":["Zarrei"],"initials":["M"],"affiliation":["The Centre for Applied Genomics (TCAG), The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Rm. 139715, 686 Bay St., Toronto, Ontario, M5G 0A4."]},{"fullName":["Kuzmina M"],"firstName":["Maria"],"lastName":["Kuzmina"],"initials":["M"],"affiliation":["Department of Botany, MRC-166 National Museum of Natural History Smithsonian Institution Rm W106 Washington, DC 20013-7012 USA."]},{"fullName":["Talent N"],"firstName":["Nadia"],"lastName":["Talent"],"initials":["N"],"affiliation":["Green Plant Herbarium (TRT), Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario Canada M5S 2C6."]},{"fullName":["Lin C"],"firstName":["Charlotte"],"lastName":["Lin"],"initials":["C"],"affiliation":["Green Plant Herbarium (TRT), Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario Canada M5S 2C6 ; School of Education, University of Stirling, Scotland UK FK9 4LA."]},{"fullName":["Dickinson TA"],"firstName":["Timothy A"],"lastName":["Dickinson"],"initials":["TA"],"affiliation":["Green Plant Herbarium (TRT), Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario Canada M5S 2C6 ; Department of Ecology & Evolutionary Biology, University of Toronto, 25 Willcocks St., Toronto, Ontario, Canada M5S 3B2."]}]}],"journalInfo":[{"issue":["36"],"journalIssueId":["2161247"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PhytoKeys"],"ISOAbbreviation":["PhytoKeys"],"medlineAbbreviation":["PhytoKeys"],"NLMid":["101548554"],"ISSN":["1314-2011"],"ESSN":["1314-2003"]}]}],"pubYear":["2014"],"pageInfo":["1-26"],"abstractText":["Crataegus monogyna Jacq. is naturalized in North America, where it has hybridized with native diploid hawthorns at least twice. We provide names for the two nothospecies (as well as for the corresponding nothosections and nothoseries), referring to existing documentation in the literature for nothosp. nov. Crataegus ×ninae-celottiae K.I. Chr. & T.A. Dickinson (C. monogyna × C. punctata Jacq.). New data are provided to further document nothosp. nov. Crataegus ×cogswellii K.I. Chr. & T.A. Dickinson (C. monogyna × C. suksdorfii (Sarg.) Kruschke). In both cases, the striking differences in leaf shape between most New World hawthorns and Old World section Crataegus, and the intermediacy of the hybrids, account for the relative ease with which these hybrids can be recognized. Finally, new sequence data from ITS2 and chloroplast DNA barcoding loci confirm the genetic relationships between the two nothospecies and their respective parents."],"affiliation":["Assoc. Prof. M.Sc. Ph.D. Knud Ib Christensen (born 13 October 1955, deceased 16 January 2012), formerly at the Botanical Garden, Natural History Museum of Denmark, University of Copenhagen."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Diploid","Hybridization","Leaf shape","Hawthorn","ITS2","North America","Dna Barcodes"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4023339?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4023339"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24843290"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24843290&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3897/phytokeys.36.6784"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"," gen"," "]}],"dateOfCompletion":["2014-05-20"],"dateOfCreation":["2014-05-20"],"dateOfRevision":["2014-05-23"],"electronicPublicationDate":["2014-04-09"],"firstPublicationDate":["2014-04-09"],"cprojectID":"PMC4023339"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqnlJ9hGurOzZIYQ","_score":1,"_source":{"id":["25926808"],"source":["MED"],"pmid":["25926808"],"pmcid":["PMC4396129"],"doi":["10.3389/fpsyg.2015.00443"],"title":["The role of personal purpose and personal goals in symbiotic visions."],"authorString":["Berg JL."],"authorList":[{"author":[{"fullName":["Berg JL"],"firstName":["Jodi L"],"lastName":["Berg"],"initials":["JL"],"affiliation":["Doctor of Management Programs, Weatherhead School of Management, Case Western Reserve University Cleveland, OH, USA."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2250732"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Frontiers in psychology"],"ISOAbbreviation":["Front Psychol"],"medlineAbbreviation":["Front Psychol"],"NLMid":["101550902"],"ESSN":["1664-1078"]}]}],"pubYear":["2015"],"pageInfo":["443"],"abstractText":["It is believed that symbiotic visions can drive employees and organizations toward a common objective based on the premise that people have a high level of self-motivation and engagement when they are working toward something very personal. The field of organizational development has been aspiring to help organizations and people align their visions for decades without much, if any, empirical support for the role of personal purpose and goals in the symbiotic relationship with a company vision. This qualitative study examines the role personal purpose and goals play in how high performing leaders align to their company's vision. Whether and how senior managers articulate this alignment, and its correlation to their motivation and engagement, was examined. An observation was that most senior managers within organizations with a well-developed and widely known higher purpose vision are driven by something personal, identified as either personal goals or a personal purpose. One of the key findings is that personal purpose and goals, when aligned to a company vision, appear to impact motivation and engagement in different ways. When alignment is felt through the sense of the greater purpose, there is a deep, almost spiritual, commitment to making the world a better place and helping the organization contribute to that. This seems to motivate them to guide the organization toward its higher purpose vision. When alignment is felt through the organization's alignment to one's personal goals, there is a great sense of commitment to completing the steps or tasks necessary to move toward the vision, yet a clear delineation between work and life ambitions."],"affiliation":["Doctor of Management Programs, Weatherhead School of Management, Case Western Reserve University Cleveland, OH, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Vision","Relationships","Calling","meaning","Motivation","Engagement","Shared Vision","Higher Purpose"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4396129?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4396129"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fpsyg.2015.00443"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-04-30"],"dateOfCreation":["2015-04-30"],"dateOfRevision":["2015-05-02"],"electronicPublicationDate":["2015-04-14"],"firstPublicationDate":["2015-04-14"],"cprojectID":"PMC4396129"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqnyJ9hGurOzZIYS","_score":1,"_source":{"id":["26075598"],"source":["MED"],"pmid":["26075598"],"pmcid":["PMC4468249"],"doi":["10.1371/journal.pone.0129682"],"title":["Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection."],"authorString":["Abd El Wahed A, Patel P, Faye O, Thaloengsok S, Heidenreich D, Matangkasombut P, Manopwisedjaroen K, Sakuntabhai A, Sall AA, Hufert FT, Weidmann M."],"authorList":[{"author":[{"fullName":["Abd El Wahed A"],"firstName":["Ahmed"],"lastName":["Abd El Wahed"],"initials":["A"],"affiliation":["Unit of Infection Models, German Primate Center, Goettingen, Germany; Department of Virology, Mansoura University, Dakahlia, Egypt."]},{"fullName":["Patel P"],"firstName":["Pranav"],"lastName":["Patel"],"initials":["P"],"affiliation":["CBS1-Highly Pathogenic Viruses, Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Arbovirus Unit, Pasteur Institute, Dakar, Senegal."]},{"fullName":["Thaloengsok S"],"firstName":["Sasikanya"],"lastName":["Thaloengsok"],"initials":["S"],"affiliation":["Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand."]},{"fullName":["Heidenreich D"],"firstName":["Doris"],"lastName":["Heidenreich"],"initials":["D"],"authorId":[{"_":"0000-0002-2010-9017","$":{"type":"ORCID"}}],"affiliation":["Department of Virology, University Medical Center, Goettingen, Germany."]},{"fullName":["Matangkasombut P"],"firstName":["Ponpan"],"lastName":["Matangkasombut"],"initials":["P"],"affiliation":["Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Systems Biology of Diseases Research Unit at Faculty of Science and Center of Emerging and Neglected Infectious Diseases, Mahidol University, Bangkok, Thailand."]},{"fullName":["Manopwisedjaroen K"],"firstName":["Khajohnpong"],"lastName":["Manopwisedjaroen"],"initials":["K"],"affiliation":["Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand."]},{"fullName":["Sakuntabhai A"],"firstName":["Anavaj"],"lastName":["Sakuntabhai"],"initials":["A"],"affiliation":["Functional Genetics of Infectious Diseases Unit, Institute Pasteur, Paris, France."]},{"fullName":["Sall AA"],"firstName":["Amadou A"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Arbovirus Unit, Pasteur Institute, Dakar, Senegal."]},{"fullName":["Hufert FT"],"firstName":["Frank T"],"lastName":["Hufert"],"initials":["FT"],"affiliation":["Institute of Microbiology and Virology, Brandenburg Medical School Theodor-Fontane, Senftenberg, Brandenburg, Germany."]},{"fullName":["Weidmann M"],"firstName":["Manfred"],"lastName":["Weidmann"],"initials":["M"],"authorId":[{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}],"affiliation":["Institute of Aquaculture, University of Stirling, Stirling, Scotland, United Kingdom."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-2010-9017","$":{"type":"ORCID"}},{"_":"0000-0002-7063-7491","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2289129"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0129682"],"abstractText":["Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3´non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magnetic-bead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 °C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100%(n=41), respectively.During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],"affiliation":["Unit of Infection Models, German Primate Center, Goettingen, Germany; Department of Virology, Mansoura University, Dakahlia, Egypt."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Sensitivity and Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Reproducibility of Results"]},{"majorTopic_YN":["N"],"descriptorName":["Nucleic Acid Amplification Techniques"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Senegal"]},{"majorTopic_YN":["N"],"descriptorName":["Thailand"]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4468249?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4468249"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0129682"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-03-04"],"dateOfCreation":["2015-06-16"],"dateOfRevision":["2015-06-27"],"electronicPublicationDate":["2015-06-15"],"firstPublicationDate":["2015-06-15"],"cprojectID":"PMC4468249"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqnyhOUS2orL1IOa","_score":1,"_source":{"id":["25412181"],"source":["MED"],"pmid":["25412181"],"pmcid":["PMC4239016"],"doi":["10.1371/journal.pone.0110620"],"title":["Development, characterization and application of monoclonal antibodies against Brazilian Dengue virus isolates."],"authorString":["Zanluca C, Mazzarotto GA, Bordignon J, Duarte Dos Santos CN."],"authorList":[{"author":[{"fullName":["Zanluca C"],"firstName":["Camila"],"lastName":["Zanluca"],"initials":["C"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas (ICC/Fiocruz/PR), Curitiba, Paraná, Brasil; Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brasil."]},{"fullName":["Mazzarotto GA"],"firstName":["Giovanny Augusto Camacho Antevere"],"lastName":["Mazzarotto"],"initials":["GA"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas (ICC/Fiocruz/PR), Curitiba, Paraná, Brasil."]},{"fullName":["Bordignon J"],"firstName":["Juliano"],"lastName":["Bordignon"],"initials":["J"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas (ICC/Fiocruz/PR), Curitiba, Paraná, Brasil."]},{"fullName":["Duarte Dos Santos CN"],"firstName":["Claudia Nunes"],"lastName":["Duarte Dos Santos"],"initials":["CN"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas (ICC/Fiocruz/PR), Curitiba, Paraná, Brasil."]}]}],"journalInfo":[{"issue":["11"],"volume":["9"],"journalIssueId":["2216242"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2014"],"pageInfo":["e110620"],"abstractText":["Dengue is the most prevalent human arboviral disease. The morbidity related to dengue infection supports the need for an early, quick and effective diagnostic test. Brazil is a hotspot for dengue, but no serological diagnostic test has been produced using Brazilian dengue virus isolates. This study aims to improve the development of immunodiagnostic methods for dengue virus (DENV) detection through the production and characterization of 22 monoclonal antibodies (mAbs) against Brazilian isolates of DENV-1, -2 and -3. The mAbs include IgG2bκ, IgG2aκ and IgG1κ isotypes, and most were raised against the envelope or the pre-membrane proteins of DENV. When the antibodies were tested against the four DENV serotypes, different reactivity patterns were identified: group-specific, subcomplex specific (DENV-1, -3 and -4 and DENV-2 and -3) and dengue serotype-specific (DENV-2 or -3). Additionally, some mAbs cross-reacted with yellow fever virus (YFV), West Nile virus (WNV) and Saint Louis encephalitis virus (SLEV). None of the mAbs recognized the alphavirus Venezuelan equine encephalitis virus (VEEV). Furthermore, mAbs D3 424/8G, D1 606/A12/B9 and D1 695/12C/2H were used to develop a capture enzyme-linked immunosorbent assay (ELISA) for anti-dengue IgM detection in sera from patients with acute dengue. To our knowledge, these are the first monoclonal antibodies raised against Brazilian DENV isolates, and they may be of special interest in the development of diagnostic assays, as well as for basic research."],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas (ICC/Fiocruz/PR), Curitiba, Paraná, Brasil; Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brasil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Cell Line"]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Immunoglobulin G"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Monoclonal"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Epitopes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cross Reactions"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["Immunoglobulin G"],"registryNumber":["0"]},{"name":["Epitopes"],"registryNumber":["0"]},{"name":["Antibodies, Monoclonal"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4239016?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4239016"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0110620"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-12-24"],"dateOfCreation":["2014-11-21"],"electronicPublicationDate":["2014-11-20"],"firstPublicationDate":["2014-11-20"],"cprojectID":"PMC4239016"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqpXhOUS2orL1IOm","_score":1,"_source":{"id":["25926325"],"source":["MED"],"pmid":["25926325"],"pmcid":["PMC4480070"],"doi":["10.1093/aobpla/plv045"],"title":["DNA barcodes from four loci provide poor resolution of taxonomic groups in the genus Crataegus."],"authorString":["Zarrei M, Talent N, Kuzmina M, Lee J, Lund J, Shipley PR, Stefanović S, Dickinson TA."],"authorList":[{"author":[{"fullName":["Zarrei M"],"firstName":["Mehdi"],"lastName":["Zarrei"],"initials":["M"],"affiliation":["The Centre for Applied Genomics, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, 686 Bay St, Toronto, ON, Canada M5G 0A4."]},{"fullName":["Talent N"],"firstName":["Nadia"],"lastName":["Talent"],"initials":["N"],"affiliation":["Green Plant Herbarium, Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, Canada M5S 2C6."]},{"fullName":["Kuzmina M"],"firstName":["Maria"],"lastName":["Kuzmina"],"initials":["M"],"affiliation":["Canadian Centre for DNA Barcoding, 50 Stone Road East, Guelph, ON, Canada N1G 2W1."]},{"fullName":["Lee J"],"firstName":["Jeanette"],"lastName":["Lee"],"initials":["J"],"affiliation":["109 Lakeshore Ave., Edgewood, BC, Canada V0G 1J0."]},{"fullName":["Lund J"],"firstName":["Jensen"],"lastName":["Lund"],"initials":["J"],"affiliation":["Department of Chemistry, I.K. Barber School of Arts and Sciences, The University of British Columbia Okanagan, 3333 University Way, Kelowna, BC, Canada V1V 1V7."]},{"fullName":["Shipley PR"],"firstName":["Paul R"],"lastName":["Shipley"],"initials":["PR"],"affiliation":["Department of Chemistry, I.K. Barber School of Arts and Sciences, The University of British Columbia Okanagan, 3333 University Way, Kelowna, BC, Canada V1V 1V7."]},{"fullName":["Stefanović S"],"firstName":["Saša"],"lastName":["Stefanović"],"initials":["S"],"affiliation":["Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada M5S 3B2 Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, Canada L5L 1C6."]},{"fullName":["Dickinson TA"],"firstName":["Timothy A"],"lastName":["Dickinson"],"initials":["TA"],"affiliation":["Green Plant Herbarium, Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, ON, Canada M5S 2C6 Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada M5S 3B2 tim.dickinson@utoronto.ca."]}]}],"journalInfo":[{"volume":["7"],"journalIssueId":["2247160"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["AoB PLANTS"],"ISOAbbreviation":["AoB Plants"],"medlineAbbreviation":["AoB Plants"],"NLMid":["101539425"],"ESSN":["2041-2851"]}]}],"pubYear":["2015"],"abstractText":["DNA barcodes can facilitate identification of organisms especially when morphological characters are limited or unobservable. To what extent this potential is realized in specific groups of plants remains to be determined. Libraries of barcode sequences from well-studied authoritatively identified plants represented by herbarium voucher specimens are needed in order for DNA barcodes to serve their intended purpose, where this is possible, and to understand the reasons behind their failure to do so, when this occurs. We evaluated four loci, widely regarded as universal DNA barcodes for plants, for their utility in hawthorn species identification. Three plastid regions, matK, rbcLa and psbA-trnH, and the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA discriminate only some of the species of Crataegus that can be recognized on the basis of their morphology etc. This is, in part, because in Rosaceae tribe Maleae most individual plastid loci yield relatively little taxonomic resolution and, in part, because the effects of allopolyploidization have not been eliminated by concerted evolution of the ITS regions. Although individual plastid markers provided generally poor resolution of taxonomic groups in Crataegus, a few species were notable exceptions. In contrast, analyses of concatenated sequences of the 3 plastid barcode loci plus 11 additional plastid loci gave a well-resolved maternal phylogeny. In the ITS2 tree, different individuals of some species formed groups with taxonomically unrelated species. This is a sign of lineage sorting due to incomplete concerted evolution in ITS2. Incongruence between the ITS2 and plastid trees is best explained by hybridization between different lineages within the genus. In aggregate, limited between-species variation in plastid loci, hybridization and a lack of concerted evolution in ITS2 all combine to limit the utility of standard barcoding markers in Crataegus. These results have implications for authentication of hawthorn materials in natural health products."],"affiliation":["The Centre for Applied Genomics, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, 686 Bay St, Toronto, ON, Canada M5G 0A4."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Rosaceae","Hybridization","Polyploidy","Plastid DNA","Hawthorn","ITS2","Natural Health Products","Gametophytic Apomixis","Maleae"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4480070?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4480070"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/aobpla/plv045"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2015-06-23"],"dateOfCreation":["2015-06-23"],"dateOfRevision":["2015-06-27"],"electronicPublicationDate":["2015-04-28"],"firstPublicationDate":["2015-04-28"],"cprojectID":"PMC4480070"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqplhOUS2orL1IOn","_score":1,"_source":{"id":["26061233"],"source":["MED"],"pmid":["26061233"],"pmcid":["PMC4501423"],"doi":["10.1590/0074-02760150192"],"title":["First report of autochthonous transmission of Zika virus in Brazil."],"authorString":["Zanluca C, Melo VC, Mosimann AL, Santos GI, Santos CN, Luz K."],"authorList":[{"author":[{"fullName":["Zanluca C"],"firstName":["Camila"],"lastName":["Zanluca"],"initials":["C"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."]},{"fullName":["Melo VC"],"firstName":["Vanessa Campos Andrade de"],"lastName":["Melo"],"initials":["VC"],"affiliation":["Secretaria Estadual de Saúde do Rio Grande do Norte, Natal, RN, Brasil."]},{"fullName":["Mosimann AL"],"firstName":["Ana Luiza Pamplona"],"lastName":["Mosimann"],"initials":["AL"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."]},{"fullName":["Santos GI"],"firstName":["Glauco Igor Viana Dos"],"lastName":["Santos"],"initials":["GI"],"affiliation":["Secretaria Estadual de Saúde do Rio Grande do Norte, Natal, RN, Brasil."]},{"fullName":["Santos CN"],"firstName":["Claudia Nunes Duarte Dos"],"lastName":["Santos"],"initials":["CN"],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."]},{"fullName":["Luz K"],"firstName":["Kleber"],"lastName":["Luz"],"initials":["K"],"affiliation":["Instituto de Medicina Tropical, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil."]}]}],"journalInfo":[{"issue":["4"],"volume":["110"],"journalIssueId":["2295916"],"dateOfPublication":["2015 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2015"],"printPublicationDate":["2015-06-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2015"],"pageInfo":["569-572"],"abstractText":["In the early 2015, several cases of patients presenting symptoms of mild fever, rash, conjunctivitis and arthralgia were reported in the northeastern Brazil. Although all patients lived in a dengue endemic area, molecular and serological diagnosis for dengue resulted negative. Chikungunya virus infection was also discarded. Subsequently, Zika virus (ZIKV) was detected by reverse transcription-polymerase chain reaction from the sera of eight patients and the result was confirmed by DNA sequencing. Phylogenetic analysis suggests that the ZIKV identified belongs to the Asian clade. This is the first report of ZIKV infection in Brazil."],"affiliation":["Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Polymerase Chain Reaction"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4501423?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4501423"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760150192"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["35"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-03-04"],"dateOfCreation":["2015-06-18"],"dateOfRevision":["2016-09-08"],"electronicPublicationDate":["2015-06-09"],"firstPublicationDate":["2015-06-09"],"cprojectID":"PMC4501423"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqqH_8VqgcuJwvgT","_score":1,"_source":{"id":["25255027"],"source":["MED"],"pmid":["25255027"],"pmcid":["PMC4177891"],"doi":["10.1371/journal.pone.0108504"],"title":["Integrating the public in mosquito management: active education by community peers can lead to significant reduction in peridomestic container mosquito habitats."],"authorString":["Healy K, Hamilton G, Crepeau T, Healy S, Unlu I, Farajollahi A, Fonseca DM."],"authorList":[{"author":[{"fullName":["Healy K"],"firstName":["Kristen"],"lastName":["Healy"],"initials":["K"],"affiliation":["Center for Vector Biology, Department of Entomology, Rutgers University, New Brunswick, New Jersey, United States of America."]},{"fullName":["Hamilton G"],"firstName":["George"],"lastName":["Hamilton"],"initials":["G"],"affiliation":["Center for Vector Biology, Department of Entomology, Rutgers University, New Brunswick, New Jersey, United States of America."]},{"fullName":["Crepeau T"],"firstName":["Taryn"],"lastName":["Crepeau"],"initials":["T"],"affiliation":["Monmouth County Mosquito Extermination Commission, Eatontown, New Jersey, United States of America."]},{"fullName":["Healy S"],"firstName":["Sean"],"lastName":["Healy"],"initials":["S"],"affiliation":["Monmouth County Mosquito Extermination Commission, Eatontown, New Jersey, United States of America."]},{"fullName":["Unlu I"],"firstName":["Isik"],"lastName":["Unlu"],"initials":["I"],"affiliation":["Mercer County Mosquito Commission, West Trenton, New Jersey, United States of America."]},{"fullName":["Farajollahi A"],"firstName":["Ary"],"lastName":["Farajollahi"],"initials":["A"],"affiliation":["Mercer County Mosquito Commission, West Trenton, New Jersey, United States of America."]},{"fullName":["Fonseca DM"],"firstName":["Dina M"],"lastName":["Fonseca"],"initials":["DM"],"authorId":[{"_":"0000-0003-4726-7100","$":{"type":"ORCID"}}],"affiliation":["Center for Vector Biology, Department of Entomology, Rutgers University, New Brunswick, New Jersey, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-4726-7100","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["9"],"volume":["9"],"journalIssueId":["2195941"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2014"],"pageInfo":["e108504"],"abstractText":["Mosquito species that utilize peridomestic containers for immature development are commonly aggressive human biters, and because they often reach high abundance, create significant nuisance. One of these species, the Asian tiger mosquito Aedes albopictus, is an important vector of emerging infectious diseases, such as dengue, chikungunya, and Zika fevers. Integrated mosquito management (IMM) of Ae. albopictus is particularly difficult because it requires access to private yards in urban and suburban residences. It has become apparent that in the event of a public health concern due to this species, homeowners will have to be active participants in the control process by reducing mosquito habitats in their properties, an activity known as source reduction. However, limited attempts at quantifying the effect of source reduction by homeowners have had mixed results. Of note, many mosquito control programs in the US have some form of education outreach, however the primary approach is often passive focusing on the distribution of education materials as flyers. In 2010, we evaluated the use of active community peer education in a source reduction program, using AmeriCorps volunteers. The volunteers were mobilized over a 4-week period, in two areas with approximately 1,000 residences each in urban Mercer and suburban Monmouth counties in New Jersey, USA. The volunteers were first provided training on peridomestic mosquitoes and on basic approaches to reducing the number of container habitats for mosquito larvae in backyards. Within the two treatment areas the volunteers successfully engaged 758 separate homes. Repeated measures analysis of variance showed a significant reduction in container habitats in the sites where the volunteers actively engaged the community compared to untreated control areas in both counties. Our results suggest that active education using community peer educators can be an effective means of source reduction, and a critical tool in the arsenal against peridomestic mosquitoes."],"affiliation":["Center for Vector Biology, Department of Entomology, Rutgers University, New Brunswick, New Jersey, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Culicidae"]},{"majorTopic_YN":["N"],"descriptorName":["Arbovirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Seasons"]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Health Education"]},{"majorTopic_YN":["Y"],"descriptorName":["Public Health Practice"]},{"majorTopic_YN":["Y"],"descriptorName":["Mosquito Control"]},{"majorTopic_YN":["N"],"descriptorName":["Community Health Services"]},{"majorTopic_YN":["N"],"descriptorName":["New Jersey"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4177891?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4177891"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0108504"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-12-01"],"dateOfCreation":["2014-09-26"],"dateOfRevision":["2016-02-03"],"electronicPublicationDate":["2014-09-25"],"firstPublicationDate":["2014-09-25"],"cprojectID":"PMC4177891"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqqL_8VqgcuJwvgU","_score":1,"_source":{"id":["24944843"],"source":["MED"],"pmid":["24944843"],"pmcid":["PMC4055592"],"doi":["10.1371/currents.outbreaks.4635a54dbffba2156fb2fd76dc49f65e"],"title":["Imported zika virus infection from the cook islands into australia, 2014."],"authorString":["Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, Humphreys JL, Gair R."],"authorList":[{"author":[{"fullName":["Pyke AT"],"firstName":["Alyssa T"],"lastName":["Pyke"],"initials":["AT"],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."]},{"fullName":["Daly MT"],"firstName":["Michelle T"],"lastName":["Daly"],"initials":["MT"],"affiliation":["Tropical Public Health, Townsville, Australia."]},{"fullName":["Cameron JN"],"firstName":["Jane N"],"lastName":["Cameron"],"initials":["JN"],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."]},{"fullName":["Moore PR"],"firstName":["Peter R"],"lastName":["Moore"],"initials":["PR"],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."]},{"fullName":["Taylor CT"],"firstName":["Carmel T"],"lastName":["Taylor"],"initials":["CT"],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."]},{"fullName":["Hewitson GR"],"firstName":["Glen R"],"lastName":["Hewitson"],"initials":["GR"],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."]},{"fullName":["Humphreys JL"],"firstName":["Jan L"],"lastName":["Humphreys"],"initials":["JL"],"affiliation":["Tropical Public Health, Townsville, Australia."]},{"fullName":["Gair R"],"firstName":["Richard"],"lastName":["Gair"],"initials":["R"],"affiliation":["Tropical Public Health, Cairns, Australia."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2118930"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2014"],"abstractText":["A female resident of Townsville, Queensland, Australia has been diagnosed with Zika virus infection following a recent trip to the Cook Islands. An initial serum sample collected in March, 2014 was positive by two separate Zika virus TaqMan real-time RT-PCRs and a pan-Flavivirus RT-PCR. Nucleotide sequencing and phylogenetics of the complete Cook Islands Zika virus envelope gene revealed 99.1% homology with a previous Cambodia 2010 sequence within the Asian lineage. In addition, IgG and IgM antibody seroconversions were detected between paired acute and convalescent phase sera using recombinant Zika virus serology assays. This is the first known imported case of Zika virus infection into northern Queensland where the potential mosquito vector Aedes aegypti is present and only the second such reported case diagnosed within Australia."],"affiliation":["Forensic and Scientific Services, Coopers Plains, Queensland, Australia."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Infectious disease","Arbovirus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4055592"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24944843"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.4635a54dbffba2156fb2fd76dc49f65e"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2014-06-19"],"dateOfCreation":["2014-06-19"],"dateOfRevision":["2014-06-23"],"electronicPublicationDate":["2014-06-02"],"firstPublicationDate":["2014-06-02"],"cprojectID":"PMC4055592"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsphOUS2orL1IOq","_score":1,"_source":{"id":["26247366"],"source":["MED"],"pmid":["26247366"],"pmcid":["PMC4527740"],"doi":["10.1371/journal.pone.0134975"],"title":["The Usual Suspects: Comparison of the Relative Roles of Potential Urban Chikungunya Virus Vectors in Australia."],"authorString":["Jansen CC, Williams CR, van den Hurk AF."],"authorList":[{"author":[{"fullName":["Jansen CC"],"firstName":["Cassie C"],"lastName":["Jansen"],"initials":["CC"],"affiliation":["Metro North Public Health Unit, Queensland Health, Windsor, Queensland, Australia."]},{"fullName":["Williams CR"],"firstName":["Craig R"],"lastName":["Williams"],"initials":["CR"],"affiliation":["Sansom Institute for Health Research, University of South Australia, Adelaide, South Australia, Australia."]},{"fullName":["van den Hurk AF"],"firstName":["Andrew F"],"lastName":["van den Hurk"],"initials":["AF"],"affiliation":["Forensic and Scientific Services, Department of Health, Queensland Government, Coopers Plains, Queensland, Australia."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2309735"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0134975"],"abstractText":["The global re-emergence of chikungunya virus (CHIKV) over the last decade presents a serious public health risk to Australia. An increasing number of imported cases further underline the potential for local transmission to occur if local mosquitoes bite an infected traveller. Laboratory experiments have identified a number of competent Australian mosquito species, including the primary vectors of CHIKV abroad, Aedes aegypti and Aedes albopictus, and local endemic species Aedes vigilax and Aedes notoscriptus. The implication of these additional endemic species as potential vectors has generated much uncertainty amongst public health professionals regarding their actual role in CHIKV transmission in the field. Using data estimated from or documented in the literature, we parameterise a simple vectorial capacity model to evaluate the relative roles of Australian mosquito species in potential CHIKV transmission. The model takes into account a number of key biological and ecological variables which influence the role of a species in field transmission, including population density, human feeding rates, mosquito survival rates and vector competence. We confirm the relative importance of Ae. aegypti and Ae. albopictus in sustaining potential CHIKV transmission in Australia. Even at maximum estimated densities and human feeding rates, Ae. vigilax and Ae. notoscriptus are likely to play a relatively minor role in CHIKV transmission, when compared with either Ae. aegypti or Ae. albopictus. This relatively straightforward analysis has application for any region where mosquito species have been incriminated in vector competence experiments, but where their actual role in CHIKV transmission has not been established."],"affiliation":["Metro North Public Health Unit, Queensland Health, Windsor, Queensland, Australia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Comparative Study"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Models, Statistical"]},{"majorTopic_YN":["N"],"descriptorName":["Cities"]},{"majorTopic_YN":["Y"],"descriptorName":["Insect Vectors"]},{"majorTopic_YN":["N"],"descriptorName":["Species Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Australia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4527740?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4527740"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0134975"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-06"],"dateOfCreation":["2015-08-07"],"dateOfRevision":["2015-08-14"],"electronicPublicationDate":["2015-08-06"],"firstPublicationDate":["2015-08-06"],"cprojectID":"PMC4527740"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqtLhOUS2orL1IOs","_score":1,"_source":{"id":["26463204"],"source":["MED"],"pmid":["26463204"],"pmcid":["PMC4553499"],"doi":["10.3390/insects6020576"],"title":["Aedes aegypti Control Strategies in Brazil: Incorporation of New Technologies to Overcome the Persistence of Dengue Epidemics."],"authorString":["Araújo HR, Carvalho DO, Ioshino RS, Costa-da-Silva AL, Capurro ML."],"authorList":[{"author":[{"fullName":["Araújo HR"],"firstName":["Helena R C"],"lastName":["Araújo"],"initials":["HR"],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. helenarca@gmail.com."]},{"fullName":["Carvalho DO"],"firstName":["Danilo O"],"lastName":["Carvalho"],"initials":["DO"],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. cdanilo@usp.br."]},{"fullName":["Ioshino RS"],"firstName":["Rafaella S"],"lastName":["Ioshino"],"initials":["RS"],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. myxelia@gmail.com."]},{"fullName":["Costa-da-Silva AL"],"firstName":["André L"],"lastName":["Costa-da-Silva"],"initials":["AL"],"authorId":[{"_":"0000-0002-7615-7376","$":{"type":"ORCID"}}],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. alcosta@icb.usp.br."]},{"fullName":["Capurro ML"],"firstName":["Margareth L"],"lastName":["Capurro"],"initials":["ML"],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. mcapurro@icb.usp.br."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7615-7376","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["6"],"journalIssueId":["2333455"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Insects"],"ISOAbbreviation":["Insects"],"medlineAbbreviation":["Insects"],"NLMid":["101574235"],"ESSN":["2075-4450"]}]}],"pubYear":["2015"],"pageInfo":["576-594"],"abstractText":["Dengue is considered to be the most important mosquito-borne viral disease in the world. The Aedes aegypti mosquito, its vector, is highly anthropophilic and is very well adapted to urban environments. Although several vaccine candidates are in advanced stages of development no licensed dengue vaccine is yet available. As a result, controlling the spread of dengue still requires that mosquitoes be targeted directly. We review the current methods of dengue vector control focusing on recent technical advances. We first examine the history of Brazil's National Dengue Control Plan in effect since 2002, and we describe its establishment and operation. With the persistent recurrence of dengue epidemics, current strategies should be reassessed to bring to the forefront a discussion of the possible implementation of new technologies in Brazil's mosquito control program."],"affiliation":["Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP 05508-000, Brazil. helenarca@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Brazil","Vector Control","Integrated Mosquito Management","Pncd"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4553499"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/insects6020576"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-10-14"],"dateOfCreation":["2015-10-14"],"dateOfRevision":["2015-10-17"],"electronicPublicationDate":["2015-06-11"],"firstPublicationDate":["2015-06-11"],"cprojectID":"PMC4553499"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnquB_8VqgcuJwvgd","_score":1,"_source":{"id":["25722614"],"source":["MED"],"pmid":["25722614"],"pmcid":["PMC4338443"],"doi":["10.4103/0974-777x.150884"],"title":["Risk assessment for yellow Fever in Western and north-Western provinces of zambia."],"authorString":["Babaniyi OA, Mwaba P, Mulenga D, Monze M, Songolo P, Mazaba-Liwewe ML, Mweene-Ndumba I, Masaninga F, Chizema E, Eshetu-Shibeshi M, Malama C, Rudatsikira E, Siziya S."],"authorList":[{"author":[{"fullName":["Babaniyi OA"],"firstName":["Olusegun A"],"lastName":["Babaniyi"],"initials":["OA"],"affiliation":["Zambia Country Office, World Health Organization, Lusaka, Zambia."]},{"fullName":["Mwaba P"],"firstName":["Peter"],"lastName":["Mwaba"],"initials":["P"],"affiliation":["Permanent Secretary Office, Ministry of Home Affairs, Lusaka, Zambia."]},{"fullName":["Mulenga D"],"firstName":["David"],"lastName":["Mulenga"],"initials":["D"],"affiliation":["Department of Clinical Sciences, School of Medicine, Copperbelt University, Ndola, Zambia."]},{"fullName":["Monze M"],"firstName":["Mwaka"],"lastName":["Monze"],"initials":["M"],"affiliation":["Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia."]},{"fullName":["Songolo P"],"firstName":["Peter"],"lastName":["Songolo"],"initials":["P"],"affiliation":["Department of Disease Prevention and Control, World Health Organization, Lusaka, Zambia."]},{"fullName":["Mazaba-Liwewe ML"],"firstName":["Mazyanga L"],"lastName":["Mazaba-Liwewe"],"initials":["ML"],"affiliation":["Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia ; Department of Immunization Vaccines and Emergencies, World Health Organization, Lusaka, Zambia."]},{"fullName":["Mweene-Ndumba I"],"firstName":["Idah"],"lastName":["Mweene-Ndumba"],"initials":["I"],"affiliation":["Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia ; Department of Immunization Vaccines and Emergencies, World Health Organization, Lusaka, Zambia."]},{"fullName":["Masaninga F"],"firstName":["Freddie"],"lastName":["Masaninga"],"initials":["F"],"affiliation":["Department of Malaria, World Health Organization, Lusaka, Zambia."]},{"fullName":["Chizema E"],"firstName":["Elizabeth"],"lastName":["Chizema"],"initials":["E"],"affiliation":["Department of Public Health Surveillance and Research, Ministry of Health, Lusaka, Zambia."]},{"fullName":["Eshetu-Shibeshi M"],"firstName":["Messeret"],"lastName":["Eshetu-Shibeshi"],"initials":["M"],"affiliation":["Department of Inter-country Support Team, World Health Organization, Harare, Zimbabwe."]},{"fullName":["Malama C"],"firstName":["Costantine"],"lastName":["Malama"],"initials":["C"],"affiliation":["Department of Public Health Surveillance and Research, Ministry of Health, Lusaka, Zambia."]},{"fullName":["Rudatsikira E"],"firstName":["Emmanuel"],"lastName":["Rudatsikira"],"initials":["E"],"affiliation":["Deans Office, School of Health Professions, Andrews University, Michigan, USA."]},{"fullName":["Siziya S"],"firstName":["Seter"],"lastName":["Siziya"],"initials":["S"],"affiliation":["Department of Clinical Sciences, School of Medicine, Copperbelt University, Ndola, Zambia ; Department of Public Health, School of Health Sciences, University of Lusaka, Lusaka, Zambia."]}]}],"journalInfo":[{"issue":["1"],"volume":["7"],"journalIssueId":["2257801"],"dateOfPublication":["2015 Jan-Mar"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Journal of global infectious diseases"],"ISOAbbreviation":["J Glob Infect Dis"],"medlineAbbreviation":["J Glob Infect Dis"],"NLMid":["101521436"],"ISSN":["0974-777X"],"ESSN":["0974-8245"]}]}],"pubYear":["2015"],"pageInfo":["11-17"],"abstractText":["BACKGROUND: North-Western and Western provinces of Zambia were reclassified as low-risk areas for yellow fever (YF). However, the current potential for YF transmission in these areas is unclear. AIMS: To determine the current potential risk of YF infection. SETTING AND DESIGN: A cross sectional study was conducted in North-Western and Western provinces of Zambia. MATERIALS AND METHODS: Samples were tested for both YF virus-specific IgG and IgM antibodies by the ELISA and YF virus confirmation was done using Plaque Reduction Neutralization Test. The samples were also tested for IgG and IgM antibodies against other flaviviruses. RESULTS: Out of the 3625 respondents who participated in the survey, 46.7% were males and 9.4% were aged less than 5 years. Overall, 58.1% of the participants slept under an impregnated insecticide-treated net and 20.6% reported indoor residual spraying of insecticides. A total of 616 (17.0%) samples were presumptive YF positive. The prevalence for YF was 0.3% for long-term infection and 0.2% for recent YF infection. None of the YF confirmed cases had received YF vaccine. Prevalence rates for other flaviviruses were 149 (4.1%) for Dengue, 370 (10.2%) for West Nile and 217 (6.0%) for Zika. CONCLUSION: There is evidence of past and recent infection of YF in both provinces. Hence, they are at a low risk for YF infection. Yellow fever vaccination should be included in the EPI program in the two provinces and strengthen surveillance with laboratory confirmation."],"affiliation":["Zambia Country Office, World Health Organization, Lusaka, Zambia."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Prevalence","Yellow Fever","Zambia","Western And North-western Provinces"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4338443"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0974-777X.150884"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-02-27"],"dateOfCreation":["2015-02-27"],"dateOfRevision":["2015-03-01"],"firstPublicationDate":["2015-01-01"],"cprojectID":"PMC4338443"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqwkJ9hGurOzZIYk","_score":1,"_source":{"id":["26401719"],"source":["MED"],"pmid":["26401719"],"pmcid":["PMC4593454"],"doi":["10.3201/eid2110.150847"],"title":["Zika Virus Outbreak, Bahia, Brazil."],"authorString":["Campos GS, Bandeira AC, Sardi SI."],"authorList":[{"author":[{"fullName":["Campos GS"],"firstName":["Gubio S"],"lastName":["Campos"],"initials":["GS"]},{"fullName":["Bandeira AC"],"firstName":["Antonio C"],"lastName":["Bandeira"],"initials":["AC"]},{"fullName":["Sardi SI"],"firstName":["Silvia I"],"lastName":["Sardi"],"initials":["SI"]}]}],"journalInfo":[{"issue":["10"],"volume":["21"],"journalIssueId":["2326551"],"dateOfPublication":["2015 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2015"],"printPublicationDate":["2015-10-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2015"],"pageInfo":["1885-1886"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Geography"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]}]}],"keywordList":[{"keyword":["Viruses","Detection","Reverse transcription PCR","Diagnosis","Brazil","outbreak","Maculopapular Rash","Bahia","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4593454?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4593454"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2110.150847"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["26403318"],"source":["MED"],"reference":["Emerg Infect Dis. 2015 Oct;21(10):1887"],"type":["Comment in"],"orderIn":["10"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["31"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-25"],"dateOfCreation":["2015-09-25"],"dateOfRevision":["2015-10-07"],"firstPublicationDate":["2015-10-01"],"cprojectID":"PMC4593454"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq3tJ9hGurOzZIYz","_score":1,"_source":{"id":["27330849"],"source":["MED"],"pmid":["27330849"],"pmcid":["PMC4747681"],"doi":["10.1371/currents.outbreaks.c97507e3e48efb946401755d468c28b2"],"title":["Epidemiology of Chikungunya Virus in Bahia, Brazil, 2014-2015."],"authorString":["Rodrigues Faria N, Lourenço J, Marques de Cerqueira E, Maia de Lima M, Pybus O, Carlos Junior Alcantara L."],"authorList":[{"author":[{"fullName":["Rodrigues Faria N"],"firstName":["Nuno"],"lastName":["Rodrigues Faria"],"initials":["N"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Lourenço J"],"firstName":["José"],"lastName":["Lourenço"],"initials":["J"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Marques de Cerqueira E"],"firstName":["Erenilde"],"lastName":["Marques de Cerqueira"],"initials":["E"],"affiliation":["Centre of Post-Graduation in Collective Health, Department of Health, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, Brazil."]},{"fullName":["Maia de Lima M"],"firstName":["Maricélia"],"lastName":["Maia de Lima"],"initials":["M"],"affiliation":["Centre of Post-Graduation in Collective Health, Department of Health, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, Brazil."]},{"fullName":["Pybus O"],"firstName":["Oliver"],"lastName":["Pybus"],"initials":["O"],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."]},{"fullName":["Carlos Junior Alcantara L"],"firstName":["Luiz"],"lastName":["Carlos Junior Alcantara"],"initials":["L"],"affiliation":["FIOCRUZ, Laboratory of Haematology, Genetics and Computational Biology, Salvador, Bahia, Brazil."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["Chikungunya is an emerging arbovirus that is characterized into four lineages. One of these, the Asian genotype, has spread rapidly in the Americas after its introduction in the Saint Martin island in October 2013. Unexpectedly, a new lineage, the East-Central-South African genotype, was introduced from Angola in the end of May 2014 in Feira de Santana (FSA), the second largest city in Bahia state, Brazil, where over 5,500 cases have now been reported. Number weekly cases of clinically confirmed CHIKV in FSA were analysed alongside with urban district of residence of CHIKV cases reported between June 2014 and October collected from the municipality's surveillance network. The number of cases per week from June 2014 until September 2015 reveals two distinct transmission waves. The first wave ignited in June and transmission ceased by December 2014. However, a second transmission wave started in January and peaked in May 2015, 8 months after the first wave peak, and this time in phase with Dengue virus and Zika virus transmission, which ceased when minimum temperature dropped to approximately 15°C. We find that shorter travelling times from the district where the outbreak first emerged to other urban districts of FSA were strongly associated with incidence in each district in 2014 (R(2))."],"affiliation":["Department of Zoology, University of Oxford, Oxford, United Kingdom."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Virus","Chikungunya","Disease Outbreak","Epidemiology And Surveillance"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4747681"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.c97507e3e48efb946401755d468c28b2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-22"],"dateOfCreation":["2016-06-22"],"dateOfRevision":["2016-06-24"],"electronicPublicationDate":["2016-02-01"],"firstPublicationDate":["2016-02-01"],"cprojectID":"PMC4747681"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq6F_8VqgcuJwvg2","_score":1,"_source":{"id":["26462422"],"source":["MED"],"pmid":["26462422"],"pmcid":["PMC4553466"],"doi":["10.3390/insects4030287"],"title":["Virology, Epidemiology and Pathology of Glossina Hytrosavirus, and Its Control Prospects in Laboratory Colonies of the Tsetse Fly, Glossina pallidipes (Diptera; Glossinidae)."],"authorString":["Kariithi HM, van Oers MM, Vlak JM, Vreysen MJ, Parker AG, Abd-Alla AM."],"authorList":[{"author":[{"fullName":["Kariithi HM"],"firstName":["Henry M"],"lastName":["Kariithi"],"initials":["HM"],"affiliation":["Biotechnology Centre, Kenya Agricultural Research Institute, Waiyaki Way, P.O. Box 14733-00100, Nairobi, Kenya. henry.kariithi@wur.nl."]},{"fullName":["van Oers MM"],"firstName":["Monique M"],"lastName":["van Oers"],"initials":["MM"],"affiliation":["Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, Wageningen 6708 PB, The Netherlands. monique.vanoers@wur.nl."]},{"fullName":["Vlak JM"],"firstName":["Just M"],"lastName":["Vlak"],"initials":["JM"],"affiliation":["Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, Wageningen 6708 PB, The Netherlands. just.vlak@wur.nl."]},{"fullName":["Vreysen MJ"],"firstName":["Marc J B"],"lastName":["Vreysen"],"initials":["MJ"],"affiliation":["Insect Pest Control Laboratories, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagrammer Strasse 5, P.O. Box 100, 1400 Vienna, Austria. m.vreysen@iaea.org."]},{"fullName":["Parker AG"],"firstName":["Andrew G"],"lastName":["Parker"],"initials":["AG"],"authorId":[{"_":"0000-0001-8187-1278","$":{"type":"ORCID"}}],"affiliation":["Insect Pest Control Laboratories, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagrammer Strasse 5, P.O. Box 100, 1400 Vienna, Austria. a.g.parker@iaea.org."]},{"fullName":["Abd-Alla AM"],"firstName":["Adly M M"],"lastName":["Abd-Alla"],"initials":["AM"],"authorId":[{"_":"0000-0001-7540-4462","$":{"type":"ORCID"}}],"affiliation":["Insect Pest Control Laboratories, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagrammer Strasse 5, P.O. Box 100, 1400 Vienna, Austria. a.m.m.abd-alla@iaea.org."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-8187-1278","$":{"type":"ORCID"}},{"_":"0000-0001-7540-4462","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["3"],"volume":["4"],"journalIssueId":["2333446"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Insects"],"ISOAbbreviation":["Insects"],"medlineAbbreviation":["Insects"],"NLMid":["101574235"],"ESSN":["2075-4450"]}]}],"pubYear":["2013"],"pageInfo":["287-319"],"abstractText":["The Glossina hytrosavirus (family Hytrosaviridae) is a double-stranded DNA virus with rod-shaped, enveloped virions. Its 190 kbp genome encodes 160 putative open reading frames. The virus replicates in the nucleus, and acquires a fragile envelope in the cell cytoplasm. Glossina hytrosavirus was first isolated from hypertrophied salivary glands of the tsetse fly, Glossina pallidipes Austen (Diptera; Glossinidae) collected in Kenya in 1986. A certain proportion of laboratory G. pallidipes flies infected by Glossina hytrosavirus develop hypertrophied salivary glands and midgut epithelial cells, gonadal anomalies and distorted sex-ratios associated with reduced insemination rates, fecundity and lifespan. These symptoms are rare in wild tsetse populations. In East Africa, G. pallidipes is one of the most important vectors of African trypanosomosis, a debilitating zoonotic disease that afflicts 37 sub-Saharan African countries. There is a large arsenal of control tactics available to manage tsetse flies and the disease they transmit. The sterile insect technique (SIT) is a robust control tactic that has shown to be effective in eradicating tsetse populations when integrated with other control tactics in an area-wide integrated approach. The SIT requires production of sterile male flies in large production facilities. To supply sufficient numbers of sterile males for the SIT component against G. pallidipes, strategies have to be developed that enable the management of the Glossina hytrosavirus in the colonies. This review provides a historic chronology of the emergence and biogeography of Glossina hytrosavirus, and includes researches on the infectomics (defined here as the functional and structural genomics and proteomics) and pathobiology of the virus. Standard operation procedures for viral management in tsetse mass-rearing facilities are proposed and a future outlook is sketched."],"affiliation":["Biotechnology Centre, Kenya Agricultural Research Institute, Waiyaki Way, P.O. Box 14733-00100, Nairobi, Kenya. henry.kariithi@wur.nl."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["sterile insect technique","Glossina","Musca","Sit","Sgh","Trypanosomosis","Salivary Gland Hypertrophy","Hytrosavirus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4553466"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/insects4030287"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2015-10-14"],"dateOfCreation":["2015-10-14"],"dateOfRevision":["2015-10-22"],"electronicPublicationDate":["2013-07-02"],"firstPublicationDate":["2013-07-02"],"cprojectID":"PMC4553466"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq6whOUS2orL1IPG","_score":1,"_source":{"id":["26856329"],"source":["MED"],"pmid":["26856329"],"pmcid":["PMC4746916"],"doi":["10.1186/s13071-016-1361-4"],"title":["The Asian tiger hunts in Maputo city--the first confirmed report of Aedes (Stegomyia) albopictus (Skuse, 1895) in Mozambique."],"authorString":["Kampango A, Abílio AP."],"authorList":[{"author":[{"fullName":["Kampango A"],"firstName":["Ayubo"],"lastName":["Kampango"],"initials":["A"],"affiliation":["Laboratório de Entomologia, Instituto Nacional de Saúde (INS), Av. Eduardo Mondlane / Allende, nº 1008, C.P. 246, Maputo, Mozambique. akampango@gmail.com."]},{"fullName":["Abílio AP"],"firstName":["Ana Paula"],"lastName":["Abílio"],"initials":["AP"],"affiliation":["Laboratório de Entomologia, Instituto Nacional de Saúde (INS), Av. Eduardo Mondlane / Allende, nº 1008, C.P. 246, Maputo, Mozambique. anabilio1408@gmail.com."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2401389"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["76"],"abstractText":["Increasing evidence suggests that dengue fever is endemic in Mozambique. Larvae of both the Afrotropical vector Aedes aegypti and its subspecies, Ae. aegypti formosus, have been reported from three provinces in Mozambique, two of which recently experienced dengue outbreaks. Despite reports of the invasive Oriental vector Ae. albopictus on the islands in the Mozambique Channel and nearby Indian Ocean, the species has not yet been reported in Mozambique.Four host-seeking female mosquitoes, collected biting the authors in an urban neighbourhood of Maputo City in the late afternoon of 6 December, 2015, are herein morphologically confirmed as Ae. albopictus.This is the first report confirming the occurrence in Mozambique of Ae. albopictus, an invasive species and an important vector of human arboviruses. In view of its potential role as a vector of dengue, Chikungunya and Zika viruses, studies are urgently needed to assess the geographical expansion and relative abundance of these important vectors to better understand the potential transmission impact of arboviruses that are efficiently transmitted and globally spread by these vectors."],"affiliation":["Laboratório de Entomologia, Instituto Nacional de Saúde (INS), Av. Eduardo Mondlane / Allende, nº 1008, C.P. 246, Maputo, Mozambique. akampango@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4746916?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4746916"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1361-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-09"],"dateOfRevision":["2016-02-11"],"electronicPublicationDate":["2016-02-09"],"firstPublicationDate":["2016-02-09"],"cprojectID":"PMC4746916"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq8w_8VqgcuJwvg7","_score":1,"_source":{"id":["26403318"],"source":["MED"],"pmid":["26403318"],"pmcid":["PMC4593458"],"doi":["10.3201/eid2110.151125"],"title":["Zika Virus Transmission from French Polynesia to Brazil."],"authorString":["Musso D."],"authorList":[{"author":[{"fullName":["Musso D"],"firstName":["Didier"],"lastName":["Musso"],"initials":["D"]}]}],"journalInfo":[{"issue":["10"],"volume":["21"],"journalIssueId":["2326551"],"dateOfPublication":["2015 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2015"],"printPublicationDate":["2015-10-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2015"],"pageInfo":["1887"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Comment","Letter"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["Y"],"descriptorName":["Geography"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]}]}]}]}],"keywordList":[{"keyword":["Viruses","Mosquito","Brazil","Arbovirus","New Caledonia","Vanuatu","French Polynesia","Vector-borne Infections","Easter Island","Pacific Islands","Cook Islands","Ae. Aegypti","Zika Virus","Aedes Spp.","Zikv","Ae. Africanus","Ae. Hensilli","Ae. Luteocephalus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4593458?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4593458"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2110.151125"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2010.151125"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["26401719"],"source":["MED"],"reference":["Emerg Infect Dis. 2015 Oct;21(10):1885-6"],"type":["Comment on"],"orderIn":["5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["16"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-26"],"dateOfCreation":["2015-09-25"],"dateOfRevision":["2015-10-07"],"firstPublicationDate":["2015-10-01"],"cprojectID":"PMC4593458"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9WhOUS2orL1IPO","_score":1,"_source":{"id":["26848672"],"source":["MED"],"pmid":["26848672"],"pmcid":["PMC4772207"],"doi":["10.3390/ijerph13020187"],"title":["Mapping to Support Fine Scale Epidemiological Cholera Investigations: A Case Study of Spatial Video in Haiti."],"authorString":["Curtis A, Blackburn JK, Smiley SL, Yen M, Camilli A, Alam MT, Ali A, Morris JG."],"authorList":[{"author":[{"fullName":["Curtis A"],"firstName":["Andrew"],"lastName":["Curtis"],"initials":["A"],"affiliation":["GIS, Health & Hazards Lab, Department of Geography, Kent State University, Kent, OH 44242, USA. acurti13@kent.edu."]},{"fullName":["Blackburn JK"],"firstName":["Jason K"],"lastName":["Blackburn"],"initials":["JK"],"affiliation":["Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA. jkblackburn@ufl.edu."]},{"fullName":["Smiley SL"],"firstName":["Sarah L"],"lastName":["Smiley"],"initials":["SL"],"affiliation":["GIS, Health & Hazards Lab, Department of Geography, Kent State University at Salem, Salem, OH 44460, USA. ssmiley8@kent.edu."]},{"fullName":["Yen M"],"firstName":["Minmin"],"lastName":["Yen"],"initials":["M"],"affiliation":["Department of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111, USA. minmin.yen@tufts.edu."]},{"fullName":["Camilli A"],"firstName":["Andrew"],"lastName":["Camilli"],"initials":["A"],"affiliation":["Department of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111, USA. andrew.camilli@tufts.edu."]},{"fullName":["Alam MT"],"firstName":["Meer Taifur"],"lastName":["Alam"],"initials":["MT"],"affiliation":["Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA. mtalam@epi.ufl.edu."]},{"fullName":["Ali A"],"firstName":["Afsar"],"lastName":["Ali"],"initials":["A"],"affiliation":["Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA. afsarali@epi.ufl.edu."]},{"fullName":["Morris JG Jr"],"firstName":["J Glenn"],"lastName":["Morris"],"initials":["JG"],"affiliation":["Emerging Pathogens Institute, University of Florida, Gainesville, FL 32611, USA. jgmorris@ufl.edu."]}]}],"journalInfo":[{"issue":["2"],"volume":["13"],"journalIssueId":["2384435"],"dateOfPublication":["2016 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2016"],"printPublicationDate":["2016-02-01"],"journal":[{"title":["International journal of environmental research and public health"],"ISOAbbreviation":["Int J Environ Res Public Health"],"medlineAbbreviation":["Int J Environ Res Public Health"],"NLMid":["101238455"],"ISSN":["1661-7827"],"ESSN":["1660-4601"]}]}],"pubYear":["2016"],"pageInfo":["187"],"abstractText":["The cartographic challenge in many developing world environments suffering a high disease burden is a lack of granular environmental covariates suitable for modeling disease outcomes. As a result, epidemiological questions, such as how disease diffuses at intra urban scales are extremely difficult to answer. This paper presents a novel geospatial methodology, spatial video, which can be used to collect and map environmental covariates, while also supporting field epidemiology. An example of epidemic cholera in a coastal town of Haiti is used to illustrate the potential of this new method. Water risks from a 2012 spatial video collection are used to guide a 2014 survey, which concurrently included the collection of water samples, two of which resulted in positive lab results \"of interest\" (bacteriophage specific for clinical cholera strains) to the current cholera situation. By overlaying sample sites on 2012 water risk maps, a further fifteen proposed water sample locations are suggested. These resulted in a third spatial video survey and an additional \"of interest\" positive water sample. A potential spatial connection between the \"of interest\" water samples is suggested. The paper concludes with how spatial video can be an integral part of future fine-scale epidemiological investigations for different pathogens."],"affiliation":["GIS, Health & Hazards Lab, Department of Geography, Kent State University, Kent, OH 44242, USA. acurti13@kent.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R01AI097405"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["R01 AI097405"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Geographic information systems","Bacteriophage","Cholera","Haiti","Spatial Video"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4772207?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4772207"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijerph13020187"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-06"],"dateOfRevision":["2016-03-09"],"electronicPublicationDate":["2016-02-03"],"firstPublicationDate":["2016-02-03"],"cprojectID":"PMC4772207"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-mhOUS2orL1IPT","_score":1,"_source":{"id":["26905027"],"source":["MED"],"pmid":["26905027"],"pmcid":["PMC4777928"],"doi":["10.1038/emi.2016.37"],"title":["Aedes mosquitoes and Zika virus infection: an A to Z of emergence?"],"authorString":["Howard CR."],"authorList":[{"author":[{"fullName":["Howard CR"],"firstName":["Colin R"],"lastName":["Howard"],"initials":["CR"],"affiliation":["College of Medicine and Dentistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2370343"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Emerging microbes & infections"],"ISOAbbreviation":["Emerg Microbes Infect"],"medlineAbbreviation":["Emerg Microbes Infect"],"NLMid":["101594885"],"ESSN":["2222-1751"]}]}],"pubYear":["2016"],"pageInfo":["e16"],"affiliation":["College of Medicine and Dentistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777928?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777928"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/emi.2016.37"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-24"],"dateOfRevision":["2016-03-11"],"electronicPublicationDate":["2016-02-24"],"firstPublicationDate":["2016-02-24"],"cprojectID":"PMC4777928"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrAJ_8VqgcuJwvhF","_score":1,"_source":{"id":["26830024"],"source":["MED"],"pmid":["26830024"],"pmcid":["PMC4735964"],"doi":["10.1186/s12879-016-1348-y"],"title":["Assessing Chikungunya risk in a metropolitan area of Argentina through satellite images and mathematical models."],"authorString":["Ruiz-Moreno D."],"authorList":[{"author":[{"fullName":["Ruiz-Moreno D"],"firstName":["Diego"],"lastName":["Ruiz-Moreno"],"initials":["D"],"affiliation":["Grupo de Ecologia Computacional, Instituto de Ciencias Sociales y Administración, Universidad Nacional Arturo Jauretche, Florencio Varela, Buenos Aires, Argentina. diego.ruizmoreno.phd@gmail.com."]}]}],"journalInfo":[{"volume":["16"],"journalIssueId":["2401698"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC infectious diseases"],"ISOAbbreviation":["BMC Infect. Dis."],"medlineAbbreviation":["BMC Infect Dis"],"NLMid":["100968551"],"ESSN":["1471-2334"]}]}],"pubYear":["2016"],"pageInfo":["49"],"abstractText":["Chikungunya fever is a viral disease that recently invaded the American continent. In America, it is transmitted mainly by the mosquito Aedes aegypti, but Aedes albopictus is the main vector in other regions of the world. This work estimates the risk of disease emergence and the corresponding population at risk for the case of a naive population in the metropolitan area of Buenos Aires, the capital city of Argentina.A classic metapopulation epidemiological model, that considers human and mosquito populations, was extended in order to include different environmental signals. First, the vital rates of the mosquitoes were affected by local temperature. Second, habitat availability estimated from satellite images was used to determine the carrying capacity for local mosquito populations. Disease invasion was proposed to occur at different moments of the year. For each scenario, Monte Carlo simulations were used to estimate the risk of disease invasion and the population at risk.The risk of a Chikungunya outbreak displays strong temporal (seasonal) patterns as well as spatial variability at the level of neighborhoods in the study area. According to the model, Summer and Fall display high risk for a Chikungunya invasion. The population at risk displays less variation over the year underlying the importance of preventive actions.The ability of mapping habitat quality for vector-borne diseases allows developing risk analysis at scales that are easily manageable for public health officers. For this location, the correlation of disease risk with the season of the year and the habitat availability could provide information to develop efficient control strategies. This also underlines the importance of involving the whole community when developing control measures for Chikungunya fever and other recently invading vector-borne diseases such as Zika fever."],"affiliation":["Grupo de Ecologia Computacional, Instituto de Ciencias Sociales y Administración, Universidad Nacional Arturo Jauretche, Florencio Varela, Buenos Aires, Argentina. diego.ruizmoreno.phd@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Risk Assessment"]},{"majorTopic_YN":["N"],"descriptorName":["Seasons"]},{"majorTopic_YN":["N"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Models, Theoretical"]},{"majorTopic_YN":["N"],"descriptorName":["Urban Health"]},{"majorTopic_YN":["N"],"descriptorName":["Argentina"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Remote Sensing Technology"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4735964?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4735964"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12879-016-1348-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-15"],"dateOfCreation":["2016-02-02"],"dateOfRevision":["2016-02-04"],"electronicPublicationDate":["2016-02-01"],"firstPublicationDate":["2016-02-01"],"cprojectID":"PMC4735964"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrAa_8VqgcuJwvhJ","_score":1,"_source":{"id":["26852018"],"source":["MED"],"pmid":["26852018"],"pmcid":["PMC4744385"],"doi":["10.1186/s12936-016-1119-7"],"title":["Large-scale Anopheles arabiensis egg quantification methods for mass-rearing operations."],"authorString":["Maïga H, Damiens D, Diabaté A, Dabiré RK, Ouédraogo GA, Lees RS, Gilles JR."],"authorList":[{"author":[{"fullName":["Maïga H"],"firstName":["Hamidou"],"lastName":["Maïga"],"initials":["H"],"affiliation":["Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstraße 5, PO Box 100, 1400, Vienna, Austria. maigahamid@yahoo.fr."]},{"fullName":["Damiens D"],"firstName":["David"],"lastName":["Damiens"],"initials":["D"],"affiliation":["Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstraße 5, PO Box 100, 1400, Vienna, Austria. damiensdavid@hotmail.com."]},{"fullName":["Diabaté A"],"firstName":["Abdoulaye"],"lastName":["Diabaté"],"initials":["A"],"affiliation":["Institut de Recherche en Sciences de la Santé/Centre Muraz, BP 390, Bobo-Dioulasso, Burkina Faso. a_diabate@hotmail.com."]},{"fullName":["Dabiré RK"],"firstName":["Roch K"],"lastName":["Dabiré"],"initials":["RK"],"affiliation":["Institut de Recherche en Sciences de la Santé/Centre Muraz, BP 390, Bobo-Dioulasso, Burkina Faso. dabire_roch@hotmail.com."]},{"fullName":["Ouédraogo GA"],"firstName":["Georges A"],"lastName":["Ouédraogo"],"initials":["GA"],"affiliation":["Université Polytechnique de Bobo-Dioulasso, 01 BP 1091, Bobo-01, Burkina Faso. oga@fasonet.bf."]},{"fullName":["Lees RS"],"firstName":["Rosemary S"],"lastName":["Lees"],"initials":["RS"],"authorId":[{"_":"0000-0002-4232-9125","$":{"type":"ORCID"}}],"affiliation":["Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstraße 5, PO Box 100, 1400, Vienna, Austria. r.s.lees@iaea.org."]},{"fullName":["Gilles JR"],"firstName":["Jeremie R L"],"lastName":["Gilles"],"initials":["JR"],"affiliation":["Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstraße 5, PO Box 100, 1400, Vienna, Austria. j.gilles@iaea.org."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-4232-9125","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2401987"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Malaria journal"],"ISOAbbreviation":["Malar. J."],"medlineAbbreviation":["Malar J"],"NLMid":["101139802"],"ESSN":["1475-2875"]}]}],"pubYear":["2016"],"pageInfo":["72"],"abstractText":["The success of the sterile insect technique relies, among other things, on the continuous release of over flooding numbers of sexually competitive sterile males into the target area. To produce sufficiently large quantities of sterile males, rearing protocols need to be optimized including the development and validation of a standardized egg quantification method.Batches of 1000 freshly laid eggs collected from standard rearing cages were counted, gently dried under laboratory conditions (27 ± 1 °C, 75 ± 5 % RH) and combined so that 1000-8000 eggs were weighed, to calculate the correlation between weight and number. The actual counted egg number and the egg number estimated by weighing were further compared for samples of 1000, 3000 and 4000 eggs collected from both standard and mass-rearing cages. The effect of drying, brushing and weighing on egg hatch rate was evaluated in three samples each of 1000 fresh and 1000 dried eggs, and in batches of 1000, 3000 and 4000 dried eggs. Pupal production and adult life history traits were assessed for dried eggs hatched and reared in mass-rearing trays. Expected egg numbers and actual observed mean egg numbers were compared after gentle drying, and after applying a rapid drying method exposure to wind speed of 1.8 m/s for 30 min.A significant positive relationship between the number of dried eggs and egg weight was observed and the equation 'Weight (mg) = (0.00399 × Number of counted eggs) + 0.536 was derived. The actual counted mean egg number and the egg number estimated by weighing were similar for samples from small rearing cages but significantly lower for samples of 3000 and 4000 egg samples collected from mass-rearing cages. No negative effect of the drying, brushing and weighing process on egg hatch rate was observed. No significant difference was observed in any life history trait between adults reared from dried or from fresh eggs up to twenty-one days post emergence. The mean number of eggs counted from a given replicate's weight was significantly higher for egg batches fast dried with a suction device compared to those dried with a gentle drying method (fast: 1075 ± 9, gentle: 1024 ± 7).An equation has been derived to allow accurate quantification of dried Anopheles arabiensis eggs based on weight, enabling more accurate quantification of eggs for consistent larval rearing density to be achieved. Eggs can be dried for weighing in a manner which does not impair the quality of resulting adults."],"affiliation":["Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramerstraße 5, PO Box 100, 1400, Vienna, Austria. maigahamid@yahoo.fr."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4744385?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4744385"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12936-016-1119-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-02-08"],"dateOfRevision":["2016-02-09"],"electronicPublicationDate":["2016-02-06"],"firstPublicationDate":["2016-02-06"],"cprojectID":"PMC4744385"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnra4hOUS2orL1IQg","_score":1,"_source":{"id":["27227971"],"source":["MED"],"pmid":["27227971"],"pmcid":["PMC4882008"],"doi":["10.1371/journal.pntd.0004765"],"title":["Zika in the United States of America and a Fateful 1969 Decision."],"authorString":["Hotez PJ."],"authorList":[{"author":[{"fullName":["Hotez PJ"],"firstName":["Peter J"],"lastName":["Hotez"],"initials":["PJ"],"affiliation":["Department of Biology, Baylor University, Waco, Texas, United States of America."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2403466"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004765"],"abstractText":["The United States Gulf Coast's current risk to Zika transmitted by Aedes aegypti mosquitoes can be traced back to some important federal health policy decisions made during the 1960s."],"affiliation":["Department of Biology, Baylor University, Waco, Texas, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882008?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4882008"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004765"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-27"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-26"],"firstPublicationDate":["2016-05-26"],"cprojectID":"PMC4882008"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnra2J9hGurOzZIaT","_score":1,"_source":{"id":["27303663"],"source":["MED"],"pmid":["27303663"],"pmcid":["PMC4885858"],"doi":["10.3389/fpubh.2016.00110"],"title":["The Threat of Zika Virus in Sub-Saharan Africa - The Need to Remain Vigilant."],"authorString":["Baraka V, Kweka EJ."],"authorList":[{"author":[{"fullName":["Baraka V"],"firstName":["Vito"],"lastName":["Baraka"],"initials":["V"],"affiliation":["Tanga Research Centre, National Institute for Medical Research, Tanga, Tanzania; Global Health Institute, Gouverneur Kinsbergen Centrum, University of Antwerp, Wilrijk, Belgium."]},{"fullName":["Kweka EJ"],"firstName":["Eliningaya J"],"lastName":["Kweka"],"initials":["EJ"],"affiliation":["Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, Arusha, Tanzania; Department of Medical Parasitology and Entomology, School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, Tanzania."]}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2374588"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in public health"],"ISOAbbreviation":["Front Public Health"],"medlineAbbreviation":["Front Public Health"],"NLMid":["101616579"],"ESSN":["2296-2565"]}]}],"pubYear":["2016"],"pageInfo":["110"],"affiliation":["Tanga Research Centre, National Institute for Medical Research, Tanga, Tanzania; Global Health Institute, Gouverneur Kinsbergen Centrum, University of Antwerp, Wilrijk, Belgium."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Sub-Saharan Africa","Commentary","Zika Virus","Arboviral Infections","Diagnosis Surveillance And Control"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4885858?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4885858"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fpubh.2016.00110"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-15"],"dateOfCreation":["2016-06-15"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-05-31"],"firstPublicationDate":["2016-05-31"],"cprojectID":"PMC4885858"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrb1hOUS2orL1IQm","_score":1,"_source":{"id":["27209301"],"source":["MED"],"pmid":["27209301"],"pmcid":["PMC4887330"],"doi":["10.1007/s13238-016-0274-5"],"title":["Highly diversified Zika viruses imported to China, 2016."],"authorString":["Zhang Y, Chen W, Wong G, Bi Y, Yan J, Sun Y, Chen E, Yan H, Lou X, Mao H, Xia S, Gao GF, Shi W, Chen Z."],"authorList":[{"author":[{"fullName":["Zhang Y"],"firstName":["Yanjun"],"lastName":["Zhang"],"initials":["Y"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Chen W"],"firstName":["Wenxian"],"lastName":["Chen"],"initials":["W"],"affiliation":["Yiwu Center for Disease Control and Prevention, Yiwu, 322000, China."]},{"fullName":["Wong G"],"firstName":["Gary"],"lastName":["Wong"],"initials":["G"],"affiliation":["CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China."]},{"fullName":["Bi Y"],"firstName":["Yuhai"],"lastName":["Bi"],"initials":["Y"],"affiliation":["Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen, 518112, China."]},{"fullName":["Yan J"],"firstName":["Juying"],"lastName":["Yan"],"initials":["J"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Sun Y"],"firstName":["Yi"],"lastName":["Sun"],"initials":["Y"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Chen E"],"firstName":["Enfu"],"lastName":["Chen"],"initials":["E"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Yan H"],"firstName":["Hao"],"lastName":["Yan"],"initials":["H"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Lou X"],"firstName":["Xiuyu"],"lastName":["Lou"],"initials":["X"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Mao H"],"firstName":["Haiyan"],"lastName":["Mao"],"initials":["H"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Xia S"],"firstName":["Shichang"],"lastName":["Xia"],"initials":["S"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."]},{"fullName":["Gao GF"],"firstName":["George F"],"lastName":["Gao"],"initials":["GF"],"affiliation":["Office of Director-General, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China."]},{"fullName":["Shi W"],"firstName":["Weifeng"],"lastName":["Shi"],"initials":["W"],"affiliation":["Institute of Pathogen Biology, Taishan Medical College, Taian, 271000, China. shiwf@ioz.ac.cn."]},{"fullName":["Chen Z"],"firstName":["Zhiping"],"lastName":["Chen"],"initials":["Z"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China. zhpchen@cdc.zj.cn."]}]}],"journalInfo":[{"issue":["6"],"volume":["7"],"journalIssueId":["2413534"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Protein & cell"],"ISOAbbreviation":["Protein Cell"],"medlineAbbreviation":["Protein Cell"],"NLMid":["101532368"],"ISSN":["1674-800X"],"ESSN":["1674-8018"]}]}],"pubYear":["2016"],"pageInfo":["461-464"],"affiliation":["Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887330?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4887330"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s13238-016-0274-5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-01"],"dateOfRevision":["2016-06-16"],"firstPublicationDate":["2016-05-21"],"cprojectID":"PMC4887330"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrc3_8VqgcuJwvia","_score":1,"_source":{"id":["26702627"],"source":["MED"],"pmid":["26702627"],"pmcid":["PMC4867002"],"doi":["10.1007/s00430-015-0445-7"],"title":["Cytokine kinetics of Zika virus-infected patients from acute to reconvalescent phase."],"authorString":["Tappe D, Pérez-Girón JV, Zammarchi L, Rissland J, Ferreira DF, Jaenisch T, Gómez-Medina S, Günther S, Bartoloni A, Muñoz-Fontela C, Schmidt-Chanasit J."],"authorList":[{"author":[{"fullName":["Tappe D"],"firstName":["Dennis"],"lastName":["Tappe"],"initials":["D"],"affiliation":["German Centre for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel, Hamburg, Germany. tappe@bnitm.de."]},{"fullName":["Pérez-Girón JV"],"firstName":["José Vicente"],"lastName":["Pérez-Girón"],"initials":["JV"],"affiliation":["Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany."]},{"fullName":["Zammarchi L"],"firstName":["Lorenzo"],"lastName":["Zammarchi"],"initials":["L"],"affiliation":["Clinica Malattie Infettive, Dipartimento di Medicina Sperimentale e Clinica, Università Degli Studi di Firenze, Florence, Italy."]},{"fullName":["Rissland J"],"firstName":["Jürgen"],"lastName":["Rissland"],"initials":["J"],"affiliation":["Institute of Virology, Saarland University Medical Center, Homburg/Saar, Germany."]},{"fullName":["Ferreira DF"],"firstName":["Davis F"],"lastName":["Ferreira"],"initials":["DF"],"affiliation":["Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil."]},{"fullName":["Jaenisch T"],"firstName":["Thomas"],"lastName":["Jaenisch"],"initials":["T"],"affiliation":["Section Clinical Tropical Medicine, Department for Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany."]},{"fullName":["Gómez-Medina S"],"firstName":["Sergio"],"lastName":["Gómez-Medina"],"initials":["S"],"affiliation":["Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany."]},{"fullName":["Günther S"],"firstName":["Stephan"],"lastName":["Günther"],"initials":["S"],"affiliation":["German Centre for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel, Hamburg, Germany."]},{"fullName":["Bartoloni A"],"firstName":["Alessandro"],"lastName":["Bartoloni"],"initials":["A"],"affiliation":["Clinica Malattie Infettive, Dipartimento di Medicina Sperimentale e Clinica, Università Degli Studi di Firenze, Florence, Italy."]},{"fullName":["Muñoz-Fontela C"],"firstName":["César"],"lastName":["Muñoz-Fontela"],"initials":["C"],"affiliation":["Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany."]},{"fullName":["Schmidt-Chanasit J"],"firstName":["Jonas"],"lastName":["Schmidt-Chanasit"],"initials":["J"],"affiliation":["German Centre for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel, Hamburg, Germany."]}]}],"journalInfo":[{"issue":["3"],"volume":["205"],"journalIssueId":["2408782"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Medical microbiology and immunology"],"ISOAbbreviation":["Med. Microbiol. Immunol."],"medlineAbbreviation":["Med Microbiol Immunol"],"NLMid":["0314524"],"ISSN":["0300-8584"],"ESSN":["1432-1831"]}]}],"pubYear":["2016"],"pageInfo":["269-273"],"abstractText":["Zika virus is an emerging mosquito-borne flavivirus currently causing large epidemics in the Pacific Ocean region and Brazil. Clinically, Zika fever resembles dengue fever, but is less severe. Whereas the clinical syndrome and laboratory diagnostic procedures have been described, little attention was paid to the immunology of the disease and its possible use for clinical follow-up of patients. Here, we investigate the role of cytokines in the pathogenesis of Zika fever in travelers returning from Asia, the Pacific, and Brazil. Polyfunctional T cell activation (Th1, Th2, Th9, and Th17 response) was seen during the acute phase characterized by respective cytokine level increases, followed by a decrease in the reconvalescent phase."],"affiliation":["German Centre for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel, Hamburg, Germany. tappe@bnitm.de."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Cytokine","outbreak","Arbovirus","Flavivirus","Zika Virus","Zika Fever"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867002?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4867002"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s00430-015-0445-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-14"],"dateOfRevision":["2016-06-01"],"electronicPublicationDate":["2015-12-24"],"firstPublicationDate":["2015-12-24"],"cprojectID":"PMC4867002"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfOJ9hGurOzZIah","_score":1,"_source":{"id":["27350793"],"source":["MED"],"pmid":["27350793"],"pmcid":["PMC4898938"],"doi":["10.3332/ecancer.2016.ed55"],"title":["Paediatric cancer care in a limited-resource setting: Children's Welfare Teaching Hospital, Medical City, Baghdad."],"authorString":["Al-Hadad SA, Al-Jadiry MF, Lefko C."],"authorList":[{"author":[{"fullName":["Al-Hadad SA"],"firstName":["Salma A Naji"],"lastName":["Al-Hadad"],"initials":["SA"],"affiliation":["Oncology Unit, Children's Welfare Teaching Hospital, Medical City complex, Bab Almoaddam, Baghdad, Iraq; Baghdad Resolve: An International Collaboration to Improve Cancer Care in Iraq."]},{"fullName":["Al-Jadiry MF"],"firstName":["Mazin Faisal Farhan"],"lastName":["Al-Jadiry"],"initials":["MF"],"affiliation":["Oncology Unit, Children's Welfare Teaching Hospital, Medical City complex, Bab Almoaddam, Baghdad, Iraq; Baghdad Resolve: An International Collaboration to Improve Cancer Care in Iraq."]},{"fullName":["Lefko C"],"firstName":["Claudia"],"lastName":["Lefko"],"initials":["C"],"affiliation":["Baghdad Resolve: An International Collaboration to Improve Cancer Care in Iraq; 40 Valley St, Northampton, MA 01060, USA."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2368129"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Ecancermedicalscience"],"ISOAbbreviation":["Ecancermedicalscience"],"medlineAbbreviation":["Ecancermedicalscience"],"NLMid":["101392236"],"ESSN":["1754-6605"]}]}],"pubYear":["2016"],"pageInfo":["ed55"],"abstractText":["There has been a lot of news coming out of Iraq in recent decades, but most of it ignores the situation for people on the ground: ordinary men, women and children who continue trying to live their lives in spite of wars, economic sanctions, violence, and social, political and cultural collapse. The challenges of maintaining and sustaining health in an environment where everything-the human spirit, education and health care systems and the health-sustaining infrastructures of housing, water purification and the electric grid-is damaged or broken are enormous."],"affiliation":["Oncology Unit, Children's Welfare Teaching Hospital, Medical City complex, Bab Almoaddam, Baghdad, Iraq; Baghdad Resolve: An International Collaboration to Improve Cancer Care in Iraq."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Cancer","Children","Iraq","Social Determinants Of Health"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4898938?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4898938"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3332/ecancer.2016.ed55"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-28"],"dateOfCreation":["2016-06-28"],"dateOfRevision":["2016-06-30"],"electronicPublicationDate":["2016-05-24"],"firstPublicationDate":["2016-05-24"],"cprojectID":"PMC4898938"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrfPhOUS2orL1IQw","_score":1,"_source":{"id":["27268016"],"source":["MED"],"pmid":["27268016"],"pmcid":["PMC4895956"],"doi":["10.1186/s12916-016-0628-0"],"title":["Understanding Zika virus pathogenesis: an interview with Catherine Spong."],"authorString":["Spong CY."],"authorList":[{"author":[{"fullName":["Spong CY"],"firstName":["Catherine Y"],"lastName":["Spong"],"initials":["CY"],"affiliation":["Eunice Kennedy Shriver, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. spongc@mail.nih.gov."]}]}],"journalInfo":[{"volume":["14"],"journalIssueId":["2401375"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC medicine"],"ISOAbbreviation":["BMC Med"],"medlineAbbreviation":["BMC Med"],"NLMid":["101190723"],"ESSN":["1741-7015"]}]}],"pubYear":["2016"],"pageInfo":["84"],"abstractText":["A recent outbreak of Zika virus has been linked to fetal abnormalities in pregnant women who have been infected. The scientific community is working toward understanding Zika virus pathogenesis to better manage affected women and children. In an interview with Dr. Catherine Spong, we discuss the aims and challenges of a forthcoming longitudinal study of a cohort of pregnant women in areas of current active Zika virus transmission."],"affiliation":["Eunice Kennedy Shriver, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. spongc@mail.nih.gov."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"keywordList":[{"keyword":["Pregnancy","Infectious disease","Cohort","Infants","Neurology","Stillbirth","Microcephaly","Miscarriage","Fetal Anomaly","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4895956?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4895956"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12916-016-0628-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-06-06"],"firstPublicationDate":["2016-06-06"],"cprojectID":"PMC4895956"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhIJ9hGurOzZIao","_score":1,"_source":{"id":["27296276"],"source":["MED"],"pmid":["27296276"],"pmcid":["PMC4906732"],"doi":["10.1186/s12978-016-0190-4"],"title":["Reproductive Health is pleased to announce a mandatory open data policy in the journal."],"authorString":["Salaria N, Kenall A, Belizán JM."],"authorList":[{"author":[{"fullName":["Salaria N"],"firstName":["Natasha"],"lastName":["Salaria"],"initials":["N"],"affiliation":["BioMed Central, London, UK. Natasha.salaria@biomedcentral.com."]},{"fullName":["Kenall A"],"firstName":["Amye"],"lastName":["Kenall"],"initials":["A"],"affiliation":["BioMed Central, London, UK."]},{"fullName":["Belizán JM"],"firstName":["José M"],"lastName":["Belizán"],"initials":["JM"],"affiliation":["Institute for Clinical Effectiveness and Health Policy (IECS), London, UK."]}]}],"journalInfo":[{"issue":["1"],"volume":["13"],"journalIssueId":["2358909"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Reproductive health"],"ISOAbbreviation":["Reprod Health"],"medlineAbbreviation":["Reprod Health"],"NLMid":["101224380"],"ESSN":["1742-4755"]}]}],"pubYear":["2016"],"pageInfo":["71"],"affiliation":["BioMed Central, London, UK. Natasha.salaria@biomedcentral.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4906732?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4906732"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12978-016-0190-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-14"],"dateOfRevision":["2016-06-16"],"electronicPublicationDate":["2016-06-13"],"firstPublicationDate":["2016-06-13"],"cprojectID":"PMC4906732"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrmAJ9hGurOzZIa4","_score":1,"_source":{"id":["27350348"],"source":["MED"],"pmid":["27350348"],"pmcid":["PMC4924234"],"doi":["10.1186/s13071-016-1644-9"],"title":["Functional analysis of Orco and odorant receptors in odor recognition in Aedes albopictus."],"authorString":["Liu H, Liu T, Xie L, Wang X, Deng Y, Chen CH, James AA, Chen XG."],"authorList":[{"author":[{"fullName":["Liu H"],"firstName":["Hongmei"],"lastName":["Liu"],"initials":["H"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."]},{"fullName":["Liu T"],"firstName":["Tong"],"lastName":["Liu"],"initials":["T"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."]},{"fullName":["Xie L"],"firstName":["Lihua"],"lastName":["Xie"],"initials":["L"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."]},{"fullName":["Wang X"],"firstName":["Xiaoming"],"lastName":["Wang"],"initials":["X"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."]},{"fullName":["Deng Y"],"firstName":["Yuhua"],"lastName":["Deng"],"initials":["Y"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."]},{"fullName":["Chen CH"],"firstName":["Chun-Hong"],"lastName":["Chen"],"initials":["CH"],"affiliation":["Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli, Taiwan."]},{"fullName":["James AA"],"firstName":["Anthony A"],"lastName":["James"],"initials":["AA"],"affiliation":["Departments of Microbiology and Molecular Genetics, and Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA, 92697-3900, USA."]},{"fullName":["Chen XG"],"firstName":["Xiao-Guang"],"lastName":["Chen"],"initials":["XG"],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China. xgchen2001@hotmail.com."]}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["363"],"abstractText":["Aedes albopictus is a globally invasive mosquito and a major vector of arboviruses, including dengue, Zika and Chikungunya. Olfactory-related behaviors, particularly host-seeking, offer opportunities to disrupt the disease-transmission process. A better understanding of odorant receptors (ORs) may assist in explaining host selection and location, and contribute to novel strategy of vector control.Based on previous prediction of 158 putative odorant receptors by Ae. albopictus genome analysis, 29 AalORs were selected for tissue-specific expression profiles in the present study. AalOrco (AalOR7), AalOR10 and AalOR88, highly expressed in female olfactory tissues, were chosen for further structure predictions as well as functional validation including calcium imaging assay in human embryonic kidney (HEK293) cells and RNA interference assay in Ae. albopictus. We also conducted electrophysiological and behavioral assays in mosquitoes after RNA interference of the three genes to determine their roles in host-seeking.The results support previous conclusions that individual conventional (ORXs) and Orco can form heteromeric complexes to recognize odorants and respond to components of human volatiles in HEK293 cells. The reduction of AalOrco transcript levels led to a significant decrease in host-seeking and confusion in host preference. In contrast, AalOR10 and AalOR88 knockdown mosquitoes showed no significant behavioral differences compared with controls. The functions of conventional ORs at least AalOR10 and AalOR88 are abolished with inhibited expression of the Orco gene orthologs, along with the concomitant relevant olfactory behavior.Combining structural and functional data, we conclude that the product of the Orco gene in this mosquito is crucial for transmitting olfactory signaling and conventional ORs contribute directly to odorant recognition. Our results provide insight into the linkage between odorant receptors and host-seeking in this important vector species."],"affiliation":["Department of Pathogen Biology, Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Mosquito","HEK293 cells","heterologous expression","Olfactory"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4924234?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4924234"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1644-9"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-28"],"dateOfRevision":["2016-06-30"],"electronicPublicationDate":["2016-06-27"],"firstPublicationDate":["2016-06-27"],"cprojectID":"PMC4924234"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnroZhOUS2orL1IRN","_score":1,"_source":{"id":["27231932"],"source":["MED"],"pmid":["27231932"],"pmcid":["PMC4926172"],"doi":["10.3390/v8060152"],"title":["Adapting the Stress Response: Viral Subversion of the mTOR Signaling Pathway."],"authorString":["Le Sage V, Cinti A, Amorim R, Mouland AJ."],"authorList":[{"author":[{"fullName":["Le Sage V"],"firstName":["Valerie"],"lastName":["Le Sage"],"initials":["V"],"affiliation":["HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada. vle_sage@hotmail.com."]},{"fullName":["Cinti A"],"firstName":["Alessandro"],"lastName":["Cinti"],"initials":["A"],"affiliation":["Department of Medicine, McGill University, Montréal, QC H3A 0G4, Canada. alessandro.cinti@mail.mcgill.ca."]},{"fullName":["Amorim R"],"firstName":["Raquel"],"lastName":["Amorim"],"initials":["R"],"affiliation":["Department of Medicine, McGill University, Montréal, QC H3A 0G4, Canada. raquel.amorim@mail.mcgill.ca."]},{"fullName":["Mouland AJ"],"firstName":["Andrew J"],"lastName":["Mouland"],"initials":["AJ"],"affiliation":["Department of Medicine, McGill University, Montréal, QC H3A 0G4, Canada. andrew.mouland@mcgill.ca."]}]}],"journalInfo":[{"issue":["6"],"volume":["8"],"journalIssueId":["2412414"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Viruses"],"ISOAbbreviation":["Viruses"],"medlineAbbreviation":["Viruses"],"NLMid":["101509722"],"ESSN":["1999-4915"]}]}],"pubYear":["2016"],"abstractText":["The mammalian target of rapamycin (mTOR) is a central regulator of gene expression, translation and various metabolic processes. Multiple extracellular (growth factors) and intracellular (energy status) molecular signals as well as a variety of stressors are integrated into the mTOR pathway. Viral infection is a significant stress that can activate, reduce or even suppress the mTOR signaling pathway. Consequently, viruses have evolved a plethora of different mechanisms to attack and co-opt the mTOR pathway in order to make the host cell a hospitable environment for replication. A more comprehensive knowledge of different viral interactions may provide fruitful targets for new antiviral drugs."],"affiliation":["HIV-1 RNA Trafficking Laboratory, Lady Davis Institute at the Jewish General Hospital, Montréal, QC H3T 1E2, Canada. vle_sage@hotmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Virus","PI3K","Autophagy","Mtor","AKT","4Ebp1"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926172?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926172"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/v8060152"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-28"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-05-24"],"firstPublicationDate":["2016-05-24"],"cprojectID":"PMC4926172"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrpAJ9hGurOzZIbD","_score":1,"_source":{"id":["27418843"],"source":["MED"],"pmid":["27418843"],"pmcid":["PMC4935311"],"doi":["10.2147/idr.s108057"],"title":["Concern over Zika virus outbreak: another alarming global threat."],"authorString":["Hafiz MY, Mahmood SU, Shoaib M, Yusuf FH."],"authorList":[{"author":[{"fullName":["Hafiz MY"],"firstName":["Muhammad Yusuf"],"lastName":["Hafiz"],"initials":["MY"],"affiliation":["Dow Medical College, Dow University of Health Sciences."]},{"fullName":["Mahmood SU"],"firstName":["Syed Uzair"],"lastName":["Mahmood"],"initials":["SU"],"affiliation":["Sindh Medical College, Jinnah Sindh Medical University, Karachi, Pakistan."]},{"fullName":["Shoaib M"],"firstName":["Maria"],"lastName":["Shoaib"],"initials":["M"],"affiliation":["Dow Medical College, Dow University of Health Sciences."]},{"fullName":["Yusuf FH"],"firstName":["Farah Hafiz"],"lastName":["Yusuf"],"initials":["FH"],"affiliation":["Dow Medical College, Dow University of Health Sciences."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2372295"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infection and drug resistance"],"ISOAbbreviation":["Infect Drug Resist"],"medlineAbbreviation":["Infect Drug Resist"],"NLMid":["101550216"],"ESSN":["1178-6973"]}]}],"pubYear":["2016"],"pageInfo":["149-151"],"abstractText":["Zika virus, as highlighted by the World Health Organization in February 2016, has emerged as a public health emergency of international concern. Zika virus is enveloped and icosahedral, and has a nonsegmented, single-stranded, positive-sense RNA genome. It belongs to Flaviviridae family. Aedes aegypticus mosquito is the known vector. Transmission is anthroponotic (human to vector to human) during outbreaks, or occurs perinatally in utero, sexually, and via transfusion of infected blood. Zika virus is turning out to be a major public health concern. Not only has it shown dramatic teratogenic association and caused serious neurological concerns but it has also spread around the globe. Countries that have not yet been affected by Zika virus should adopt proper preventive methods to limit its spread in the population."],"affiliation":["Dow Medical College, Dow University of Health Sciences."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Teratogenic","Zika Virus","Public Health Emergency","Neurological Anomalies","Global Spread"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4935311?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4935311"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2147/IDR.S108057"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-15"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-06-28"],"firstPublicationDate":["2016-06-28"],"cprojectID":"PMC4935311"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrISJ9hGurOzZIZc","_score":1,"_source":{"id":["27074809"],"source":["MED"],"pmid":["27074809"],"pmcid":["PMC4831111"],"doi":["10.1186/s12936-016-1269-7"],"title":["Consolidating tactical planning and implementation frameworks for integrated vector management in Uganda."],"authorString":["Okia M, Okui P, Lugemwa M, Govere JM, Katamba V, Rwakimari JB, Mpeka B, Chanda E."],"authorList":[{"author":[{"fullName":["Okia M"],"firstName":["Michael"],"lastName":["Okia"],"initials":["M"],"affiliation":["Abt Associates, Kampala, Uganda."]},{"fullName":["Okui P"],"firstName":["Peter"],"lastName":["Okui"],"initials":["P"],"affiliation":["National Malaria Control Programme, Ministry of Health, Kampala, Uganda."]},{"fullName":["Lugemwa M"],"firstName":["Myers"],"lastName":["Lugemwa"],"initials":["M"],"affiliation":["National Malaria Control Programme, Ministry of Health, Kampala, Uganda."]},{"fullName":["Govere JM"],"firstName":["John M"],"lastName":["Govere"],"initials":["JM"],"affiliation":[", Nelspruit, Mpumalanga, South Africa."]},{"fullName":["Katamba V"],"firstName":["Vincent"],"lastName":["Katamba"],"initials":["V"],"affiliation":["National Malaria Control Programme, Ministry of Health, Kampala, Uganda."]},{"fullName":["Rwakimari JB"],"firstName":["John B"],"lastName":["Rwakimari"],"initials":["JB"],"affiliation":["Abt Associates, Kampala, Uganda."]},{"fullName":["Mpeka B"],"firstName":["Betty"],"lastName":["Mpeka"],"initials":["B"],"affiliation":["Abt Associates, Kampala, Uganda."]},{"fullName":["Chanda E"],"firstName":["Emmanuel"],"lastName":["Chanda"],"initials":["E"],"affiliation":[", Kamwala South, Lusaka, Zambia. emmanuel_chanda@yahoo.co.uk."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2401987"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Malaria journal"],"ISOAbbreviation":["Malar. J."],"medlineAbbreviation":["Malar J"],"NLMid":["101139802"],"ESSN":["1475-2875"]}]}],"pubYear":["2016"],"pageInfo":["214"],"abstractText":["Integrated vector management (IVM) is the recommended approach for controlling some vector-borne diseases (VBD). In the face of current challenges to disease vector control, IVM is vital to achieve national targets set for VBD control. Though global efforts, especially for combating malaria, now focus on elimination and eradication, IVM remains useful for Uganda which is principally still in the control phase of the malaria continuum. This paper outlines the processes undertaken to consolidate tactical planning and implementation frameworks for IVM in Uganda.The Uganda National Malaria Control Programme with its efforts to implement an IVM approach to vector control was the 'case' for this study. Integrated management of malaria vectors in Uganda remained an underdeveloped component of malaria control policy. In 2012, knowledge and perceptions of malaria vector control policy and IVM were assessed, and recommendations for a specific IVM policy were made. In 2014, a thorough vector control needs assessment (VCNA) was conducted according to WHO recommendations. The findings of the VCNA informed the development of the national IVM strategic guidelines. Information sources for this study included all available data and accessible archived documentary records on VBD control in Uganda. The literature was reviewed and adapted to the local context and translated into the consolidated tactical framework.WHO recommends implementation of IVM as the main strategy to vector control and has encouraged member states to adopt the approach. However, many VBD-endemic countries lack IVM policy frameworks to guide implementation of the approach. In Uganda most VBD coexists and could be managed more effectively if done in tandem. In order to successfully control malaria and other VBD and move towards their elimination, the country needs to scale up proven and effective vector control interventions and also learn from the experience of other countries. The IVM strategy is important in consolidating inter-sectoral collaboration and coordination and providing the tactical direction for effective deployment of vector control interventions along the five key elements of the approach and to align them with contemporary epidemiology of VBD in the country.Uganda has successfully established an evidence-based IVM approach and consolidated strategic planning and operational frameworks for VBD control. However, operating implementation arrangements as outlined in the national strategic guidelines for IVM and managing insecticide resistance, as well as improving vector surveillance, are imperative. In addition, strengthened information, education and communication/behaviour change and communication, collaboration and coordination will be crucial in scaling up and using vector control interventions."],"affiliation":["Abt Associates, Kampala, Uganda."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"keywordList":[{"keyword":["Sustainability","Uganda","Integrated Vector Management","Vector Surveillance","Malaria Vector Control","Insecticide Resistance Management","Vector Borne Disease Control","Vector Control Needs Assessment"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4831111?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4831111"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12936-016-1269-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-14"],"dateOfRevision":["2016-04-16"],"electronicPublicationDate":["2016-04-14"],"firstPublicationDate":["2016-04-14"],"cprojectID":"PMC4831111"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrJsJ9hGurOzZIZi","_score":1,"_source":{"id":["27100178"],"source":["MED"],"pmid":["27100178"],"pmcid":["PMC4839627"],"doi":["10.1371/journal.ppat.1005515"],"title":["The Tree(s) of Life: The Human Placenta and My Journey to Learn More about It."],"authorString":["Coyne CB."],"authorList":[{"author":[{"fullName":["Coyne CB"],"firstName":["Carolyn B"],"lastName":["Coyne"],"initials":["CB"],"affiliation":["Department of Microbiology and Molecular Genetics, Department of Obstetrics and Gynecology, Magee Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America."]}]}],"journalInfo":[{"issue":["4"],"volume":["12"],"journalIssueId":["2392682"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005515"],"affiliation":["Department of Microbiology and Molecular Genetics, Department of Obstetrics and Gynecology, Magee Women's Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839627?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4839627"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005515"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-22"],"dateOfRevision":["2016-04-30"],"electronicPublicationDate":["2016-04-21"],"firstPublicationDate":["2016-04-21"],"cprojectID":"PMC4839627"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrMKhOUS2orL1IPv","_score":1,"_source":{"id":["27083158"],"source":["MED"],"pmid":["27083158"],"pmcid":["PMC4833953"],"doi":["10.1186/s13071-016-1469-6"],"title":["Membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction."],"authorString":["Tan CH, Wong PS, Li MZ, Yang HT, Chong CS, Lee LK, Yuan S, Leo YS, Ng LC, Lye DC."],"authorList":[{"author":[{"fullName":["Tan CH"],"firstName":["Cheong-Huat"],"lastName":["Tan"],"initials":["CH"],"affiliation":["Faculty of Science, Monash University, Melbourne, Australia. Tan_Cheong_Huat@nea.gov.sg."]},{"fullName":["Wong PS"],"firstName":["Pei-Sze Jeslyn"],"lastName":["Wong"],"initials":["PS"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Li MZ"],"firstName":["Mei-Zhi Irene"],"lastName":["Li"],"initials":["MZ"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Yang HT"],"firstName":["Hui-Ting"],"lastName":["Yang"],"initials":["HT"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Chong CS"],"firstName":["Chee-Seng"],"lastName":["Chong"],"initials":["CS"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Lee LK"],"firstName":["Linda K"],"lastName":["Lee"],"initials":["LK"],"affiliation":["Communicable Disease Center, Tan Tock Seng Hospital, Singapore, Singapore."]},{"fullName":["Yuan S"],"firstName":["Shi"],"lastName":["Yuan"],"initials":["S"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore, Singapore."]},{"fullName":["Leo YS"],"firstName":["Yee-Sin"],"lastName":["Leo"],"initials":["YS"],"affiliation":["Communicable Disease Center, Tan Tock Seng Hospital, Singapore, Singapore."]},{"fullName":["Ng LC"],"firstName":["Lee-Ching"],"lastName":["Ng"],"initials":["LC"],"affiliation":["School of Biological Sciences (SBS), Nanyang Technological University, Singapore, Singapore."]},{"fullName":["Lye DC"],"firstName":["David C"],"lastName":["Lye"],"initials":["DC"],"affiliation":["Communicable Disease Center, Tan Tock Seng Hospital, Singapore, Singapore."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2401389"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["211"],"abstractText":["Understanding the interaction between Aedes vectors and dengue viruses (DENV) has significant implications in determining the transmission dynamics of dengue. The absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured DENV. Data obtained from such studies may not reflect the natural human-mosquito transmission scenario. This study explored the potential of using membrane feeding of dengue patient's blood as a substitute for direct skin feeding.Four to six-day old female Ae. aegypti were provided the opportunity to feed via direct exposure to a patient's forearm for 15 min or via exposure to EDTA-treated blood from the same patient through an artificial membrane for 30 min. Mosquitoes from both feeding methods were incubated inside environmental chambers. Mosquitoes were sampled at day 13 post-feeding. Midgut and salivary glands of each mosquito were dissected to determine DENV infection by RT-qPCR and viral titration, respectively.Feeding rates: Direct skin feeding assay (DSFA) consistently showed higher mosquito feeding rates (93.3-100%) when compared with the membrane feeding assay (MFA) (48-98.2%). Midgut infection: Pair-wise comparison between methods showed no significant difference in midgut infection rates between mosquitoes exposed via each method and a strong correlation was observed in midgut infection rates for both feeding methods (r = 0.89, P < 0.0001). Overall midgut viral titers (n = 20) obtained by both methods were comparable (P ≥ 0.06). Salivary gland infection: Pair-wise comparison between both methods revealed no significant difference in salivary gland infection rate. Strong correlation in salivary gland infection was observed between DSFA and MFA (r = 0.81, P < 0.0001). In general, mosquitoes fed directly on dengue patients and those on patients' blood (n = 11) had comparable virus titer (P ≥ 0.09).DENV midgut and salivary gland infection rates showed good concordance between DSFA and MFA blood meal exposure methods. Freshly-obtained venous blood in EDTA from dengue patients for MFA can be used as a substitute to DSFA, especially in circumstances where bioethics approval or patient recruitment is difficult to obtain for vector competence studies. Nevertheless, mosquito numbers will need to be increased to compensate for lower feeding rate in MFA."],"affiliation":["Faculty of Science, Monash University, Melbourne, Australia. Tan_Cheong_Huat@nea.gov.sg."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Aedes aegypti","Dengue","Edta Blood","Membrane Feeding Assay (Mfa)","Direct Skin Feeding Assay (Dfsa)"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4833953?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4833953"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1469-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-16"],"dateOfRevision":["2016-04-18"],"electronicPublicationDate":["2016-04-15"],"firstPublicationDate":["2016-04-15"],"cprojectID":"PMC4833953"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrP9hOUS2orL1IP9","_score":1,"_source":{"id":["27119012"],"source":["MED"],"pmid":["27119012"],"pmcid":["PMC4845342"],"doi":["10.1186/s13578-016-0096-4"],"title":["Studies using IPS cells support a possible link between ZIKA and microcephaly."],"authorString":["Guo J."],"authorList":[{"author":[{"fullName":["Guo J"],"firstName":["Jia"],"lastName":["Guo"],"initials":["J"],"affiliation":["National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA USA."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2367765"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Cell & bioscience"],"ISOAbbreviation":["Cell Biosci"],"medlineAbbreviation":["Cell Biosci"],"NLMid":["101561195"],"ESSN":["2045-3701"]}]}],"pubYear":["2016"],"pageInfo":["28"],"abstractText":["There is a suspected link between Brazilian babies born with microcephaly and Zika virus (ZIKV) infection. However, little is know about the brain cell targets and the mechanisms that Zika virus may cause microcephaly. A recent report demonstrated that Zika virus infection increases cell death and dysregulates cell-cycle, resulting in attenuated human neural progenitor cells growth. This study fills a major gap and serves as an entry point to establish a mechanistic link between Zika infection and microcephaly."],"affiliation":["National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Microcephaly","Human Neural Progenitor Cells","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4845342?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4845342"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13578-016-0096-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-27"],"dateOfCreation":["2016-04-27"],"dateOfRevision":["2016-04-29"],"electronicPublicationDate":["2016-04-26"],"firstPublicationDate":["2016-04-26"],"cprojectID":"PMC4845342"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrT-_8VqgcuJwvh9","_score":1,"_source":{"id":["27103718"],"source":["MED"],"pmid":["27103718"],"pmcid":["PMC4841133"],"doi":["10.1128/genomea.00291-16"],"title":["Complete Genome Sequence of Zika Virus from the First Imported Case in Mainland China."],"authorString":["Liu L, Wu W, Zhao X, Xiong Y, Zhang S, Liu X, Qu J, Li J, Nei K, Liang M, Shu Y, Hu G, Ma X, Li D."],"authorList":[{"author":[{"fullName":["Liu L"],"firstName":["Lin"],"lastName":["Liu"],"initials":["L"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Wu W"],"firstName":["Wei"],"lastName":["Wu"],"initials":["W"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Zhao X"],"firstName":["Xiang"],"lastName":["Zhao"],"initials":["X"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Xiong Y"],"firstName":["Ying"],"lastName":["Xiong"],"initials":["Y"],"affiliation":["Jiangxi Province CDC, Nanchang, China."]},{"fullName":["Zhang S"],"firstName":["Shuo"],"lastName":["Zhang"],"initials":["S"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Liu X"],"firstName":["Xiaoqing"],"lastName":["Liu"],"initials":["X"],"affiliation":["Jiangxi Province CDC, Nanchang, China."]},{"fullName":["Qu J"],"firstName":["Jing"],"lastName":["Qu"],"initials":["J"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Li J"],"firstName":["Jiandong"],"lastName":["Li"],"initials":["J"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Nei K"],"firstName":["Kai"],"lastName":["Nei"],"initials":["K"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Liang M"],"firstName":["Mifang"],"lastName":["Liang"],"initials":["M"],"authorId":[{"_":"0000-0001-7510-4435","$":{"type":"ORCID"}}],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Shu Y"],"firstName":["Yuelong"],"lastName":["Shu"],"initials":["Y"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."]},{"fullName":["Hu G"],"firstName":["Guoliang"],"lastName":["Hu"],"initials":["G"],"affiliation":["Jiangxi Province CDC, Nanchang, China."]},{"fullName":["Ma X"],"firstName":["Xuejun"],"lastName":["Ma"],"initials":["X"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China lidx@Chinacdc.cn maxj@ivdc.chinacdc.cn."]},{"fullName":["Li D"],"firstName":["Dexin"],"lastName":["Li"],"initials":["D"],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China lidx@Chinacdc.cn maxj@ivdc.chinacdc.cn."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-7510-4435","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["4"],"journalIssueId":["2382239"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome announcements"],"ISOAbbreviation":["Genome Announc"],"medlineAbbreviation":["Genome Announc"],"NLMid":["101595808"],"ESSN":["2169-8287"]}]}],"pubYear":["2016"],"abstractText":["The first case of Zika virus infection was identified in a Chinese traveler returning from Venezuela in February 2016. This report describes the complete genome sequence of Zika virus from the first imported case in China. The genome sequence analysis showed that the Zika virus isolated in this case belongs to the Asian lineage."],"affiliation":["NHFPC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841133?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841133"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00291-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-04-22"],"dateOfCreation":["2016-04-22"],"dateOfRevision":["2016-04-28"],"electronicPublicationDate":["2016-04-21"],"firstPublicationDate":["2016-04-21"],"cprojectID":"PMC4841133"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrWv_8VqgcuJwviA","_score":1,"_source":{"id":["27011352"],"source":["MED"],"pmid":["27011352"],"pmcid":["PMC4841775"],"doi":["10.7554/elife.11275"],"title":["Comparative analysis of viral RNA signatures on different RIG-I-like receptors."],"authorString":["Sanchez David RY, Combredet C, Sismeiro O, Dillies MA, Jagla B, Coppée JY, Mura M, Guerbois Galla M, Despres P, Tangy F, Komarova AV."],"authorList":[{"author":[{"fullName":["Sanchez David RY"],"firstName":["Raul Y"],"lastName":["Sanchez David"],"initials":["RY"],"affiliation":["Ecole doctorale, Biochimie, Biothérapies, Biologie Moléculaire et Infectiologie (B3MI), Université Paris Diderot - Paris 7, Paris, France."]},{"fullName":["Combredet C"],"firstName":["Chantal"],"lastName":["Combredet"],"initials":["C"],"affiliation":["Unité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France."]},{"fullName":["Sismeiro O"],"firstName":["Odile"],"lastName":["Sismeiro"],"initials":["O"],"affiliation":["Transcriptome and Epigenome, BioMics Pole, Center for Innovation and Technological Research, Institut Pasteur, Paris, France."]},{"fullName":["Dillies MA"],"firstName":["Marie-Agnès"],"lastName":["Dillies"],"initials":["MA"],"affiliation":["Transcriptome and Epigenome, BioMics Pole, Center for Innovation and Technological Research, Institut Pasteur, Paris, France."]},{"fullName":["Jagla B"],"firstName":["Bernd"],"lastName":["Jagla"],"initials":["B"],"affiliation":["Transcriptome and Epigenome, BioMics Pole, Center for Innovation and Technological Research, Institut Pasteur, Paris, France."]},{"fullName":["Coppée JY"],"firstName":["Jean-Yves"],"lastName":["Coppée"],"initials":["JY"],"affiliation":["Transcriptome and Epigenome, BioMics Pole, Center for Innovation and Technological Research, Institut Pasteur, Paris, France."]},{"fullName":["Mura M"],"firstName":["Marie"],"lastName":["Mura"],"initials":["M"],"affiliation":["Unité Interactions Hôte-Agents Pathogens, Institut de Recherche Biomédicale des Armées, Brétigny-sur-Orge, France."]},{"fullName":["Guerbois Galla M"],"firstName":["Mathilde"],"lastName":["Guerbois Galla"],"initials":["M"],"affiliation":["Unité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France."]},{"fullName":["Despres P"],"firstName":["Philippe"],"lastName":["Despres"],"initials":["P"],"affiliation":["Technology Platform CYROI, University of Reunion Island, Saint-Clotilde, France."]},{"fullName":["Tangy F"],"firstName":["Frédéric"],"lastName":["Tangy"],"initials":["F"],"affiliation":["Unité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France."]},{"fullName":["Komarova AV"],"firstName":["Anastassia V"],"lastName":["Komarova"],"initials":["AV"],"authorId":[{"_":"0000-0002-9998-8447","$":{"type":"ORCID"}}],"affiliation":["Unité de Génomique Virale et Vaccination, Institut Pasteur, CNRS UMR-3569, Paris, France."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9998-8447","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2360542"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["eLife"],"ISOAbbreviation":["Elife"],"medlineAbbreviation":["Elife"],"NLMid":["101579614"],"ESSN":["2050-084X"]}]}],"pubYear":["2016"],"pageInfo":["e11275"],"abstractText":["The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) and applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- and positive-sense RNA genome were used: measles (MV) and chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 and LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3' untranslated region of viral genome. This study provides the first comparative view of the viral RNA ligands for RIG-I, MDA5 and LGP2 in the presence of infection."],"affiliation":["Ecole doctorale, Biochimie, Biothérapies, Biologie Moléculaire et Infectiologie (B3MI), Université Paris Diderot - Paris 7, Paris, France."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Human","Microbiology","Infectious disease","PAMP","Ribonucleoproteins","immunology","innate immunity","PRR","Virus-host Interactions","Rig-i-like Receptors"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841775?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841775"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7554/eLife.11275"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-21"],"dateOfRevision":["2016-04-26"],"electronicPublicationDate":["2016-03-24"],"firstPublicationDate":["2016-03-24"],"cprojectID":"PMC4841775"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXEJ9hGurOzZIZ8","_score":1,"_source":{"id":["27089253"],"source":["MED"],"pmid":["27089253"],"pmcid":["PMC4861534"],"doi":["10.3201/eid2205.160023"],"title":["Detection of Autochthonous Zika Virus Transmission in Sincelejo, Colombia."],"authorString":["Camacho E, Paternina-Gomez M, Blanco PJ, Osorio JE, Aliota MT."],"authorList":[{"author":[{"fullName":["Camacho E"],"firstName":["Erwin"],"lastName":["Camacho"],"initials":["E"]},{"fullName":["Paternina-Gomez M"],"firstName":["Margaret"],"lastName":["Paternina-Gomez"],"initials":["M"]},{"fullName":["Blanco PJ"],"firstName":["Pedro J"],"lastName":["Blanco"],"initials":["PJ"]},{"fullName":["Osorio JE"],"firstName":["Jorge E"],"lastName":["Osorio"],"initials":["JE"]},{"fullName":["Aliota MT"],"firstName":["Matthew T"],"lastName":["Aliota"],"initials":["MT"]}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["927-929"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Viruses","Aedes aegypti","Detection","Diagnosis","Mosquito","outbreak","Colombia","Arbovirus","Vector-borne Infections","Zika Virus","Sincelejo"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861534?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861534"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.160023"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861534"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXGhOUS2orL1IQP","_score":1,"_source":{"id":["27088817"],"source":["MED"],"pmid":["27088817"],"pmcid":["PMC4861539"],"doi":["10.3201/eid2205.160107"],"title":["Detection of Zika Virus in Semen."],"authorString":["Atkinson B, Hearn P, Afrough B, Lumley S, Carter D, Aarons EJ, Simpson AJ, Brooks TJ, Hewson R."],"authorList":[{"author":[{"fullName":["Atkinson B"],"firstName":["Barry"],"lastName":["Atkinson"],"initials":["B"]},{"fullName":["Hearn P"],"firstName":["Pasco"],"lastName":["Hearn"],"initials":["P"]},{"fullName":["Afrough B"],"firstName":["Babak"],"lastName":["Afrough"],"initials":["B"]},{"fullName":["Lumley S"],"firstName":["Sarah"],"lastName":["Lumley"],"initials":["S"]},{"fullName":["Carter D"],"firstName":["Daniel"],"lastName":["Carter"],"initials":["D"]},{"fullName":["Aarons EJ"],"firstName":["Emma J"],"lastName":["Aarons"],"initials":["EJ"]},{"fullName":["Simpson AJ"],"firstName":["Andrew J"],"lastName":["Simpson"],"initials":["AJ"]},{"fullName":["Brooks TJ"],"firstName":["Timothy J"],"lastName":["Brooks"],"initials":["TJ"]},{"fullName":["Hewson R"],"firstName":["Roger"],"lastName":["Hewson"],"initials":["R"]}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["940"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Semen","Diagnostic","Sexual Transmission","Flaviviruses","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861539?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861539"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.160107"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861539"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrYthOUS2orL1IQX","_score":1,"_source":{"id":["27193266"],"source":["MED"],"pmid":["27193266"],"pmcid":["PMC4871896"],"doi":["10.3402/gha.v9.31669"],"title":["Estimated Zika virus importations to Europe by travellers from Brazil."],"authorString":["Massad E, Tan SH, Khan K, Wilder-Smith A."],"authorList":[{"author":[{"fullName":["Massad E"],"firstName":["Eduardo"],"lastName":["Massad"],"initials":["E"],"affiliation":["London School of Hygiene and Tropical Medicine, London, UK."]},{"fullName":["Tan SH"],"firstName":["Ser-Han"],"lastName":["Tan"],"initials":["SH"],"affiliation":["School of Computer Engineering, Nanyang Technological University, Singapore."]},{"fullName":["Khan K"],"firstName":["Kamran"],"lastName":["Khan"],"initials":["K"],"affiliation":["Li Ka Shing Knowledge Institute, St Michael's Hospital, Toronto, Canada."]},{"fullName":["Wilder-Smith A"],"firstName":["Annelies"],"lastName":["Wilder-Smith"],"initials":["A"],"affiliation":["Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; anneliesws@gmail.com."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2363164"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Global health action"],"ISOAbbreviation":["Glob Health Action"],"medlineAbbreviation":["Glob Health Action"],"NLMid":["101496665"],"ISSN":["1654-9716"],"ESSN":["1654-9880"]}]}],"pubYear":["2016"],"pageInfo":["31669"],"abstractText":["Given the interconnectivity of Brazil with the rest of the world, Zika virus (ZIKV) infections have the potential to spread rapidly around the world via viremic travellers. The extent of spread depends on the travel volume and the endemicity in the exporting country. In the absence of reliable surveillance data, we did mathematical modelling to estimate the number of importations of ZIKV from Brazil into Europe.We applied a previously developed mathematical model on importations of dengue to estimate the number of ZIKV importations into Europe, based on the travel volume, the probability of being infected at the time of travel, the population size of Brazil, and the estimated incidence of ZIKV infections.Our model estimated between 508 and 1,778 imported infections into Europe in 2016, of which we would expect between 116 and 355 symptomatic Zika infections; with the highest number of importations being into France, Portugal and Italy.Our model identified high-risk countries in Europe. Such data can assist policymakers and public health professionals in estimating the extent of importations in order to prepare for the scale up of laboratory diagnostic assays and estimate the occurrence of Guillain-Barré Syndrome, potential sexual transmission, and infants with congenital ZIKV syndrome."],"affiliation":["London School of Hygiene and Tropical Medicine, London, UK."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Brazil","Europe","Travel","Zika Virus","Importations"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871896?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4871896"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3402/gha.v9.31669"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-19"],"dateOfRevision":["2016-05-26"],"electronicPublicationDate":["2016-05-17"],"firstPublicationDate":["2016-05-17"],"cprojectID":"PMC4871896"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSNJ9hGurOzZIXc","_score":1,"_source":{"id":["19641608"],"source":["MED"],"pmid":["19641608"],"pmcid":["PMC2712763"],"doi":["10.1371/journal.pone.0006424"],"title":["RNase 7 contributes to the cutaneous defense against Enterococcus faecium."],"authorString":["Köten B, Simanski M, Gläser R, Podschun R, Schröder JM, Harder J."],"authorList":[{"author":[{"fullName":["Köten B"],"firstName":["Bente"],"lastName":["Köten"],"initials":["B"]},{"fullName":["Simanski M"],"firstName":["Maren"],"lastName":["Simanski"],"initials":["M"]},{"fullName":["Gläser R"],"firstName":["Regine"],"lastName":["Gläser"],"initials":["R"]},{"fullName":["Podschun R"],"firstName":["Rainer"],"lastName":["Podschun"],"initials":["R"]},{"fullName":["Schröder JM"],"firstName":["Jens-Michael"],"lastName":["Schröder"],"initials":["JM"]},{"fullName":["Harder J"],"firstName":["Jürgen"],"lastName":["Harder"],"initials":["J"]}]}],"journalInfo":[{"issue":["7"],"volume":["4"],"journalIssueId":["1633091"],"dateOfPublication":["2009 "],"monthOfPublication":["0"],"yearOfPublication":["2009"],"printPublicationDate":["2009-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2009"],"pageInfo":["e6424"],"abstractText":[" BACKGROUND: Human skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system against E. faecium. METHODOLOGY/PRINCIPAL FINDINGS: Absolute quantification using real-time PCR and ELISA revealed that primary keratinocytes expressed high levels of RNase 7. Immunohistochemistry showed RNase 7 expression in all epidermal layers of the skin with an intensification in the upper more differentiated layers. Furthermore, RNase 7 was secreted by keratinocytes in vitro and in vivo in a site-dependent way. RNase 7 was still active against E. faecium at low pH (5.5) or high NaCl (150 mM) concentration and the bactericidal activity of RNase 7 against E. faecium required no ribonuclease activity as shown by recombinant RNase 7 lacking enzymatic activity. To further explore the role of RNase 7 in cutaneous defense against E. faecium, we investigated whether RNase 7 contributes to the E. faecium killing activity of skin extracts derived from stratum corneum. Treatment of the skin extract with an RNase 7 specific antibody, which neutralizes the antimicrobial activity of RNase 7, diminished its E. faecium killing activity. CONCLUSIONS/SIGNIFICANCE: Our data indicate that RNase 7 contributes to the E. faecium-killing activity of skin extracts and suggest an important role for RNase 7 in the protection of human skin against E. faecium colonization."],"affiliation":["Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Skin"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EN"],"qualifierName":["enzymology"],"majorTopic_YN":["N"]},{"abbreviation":["MI"],"qualifierName":["microbiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Enterococcus faecium"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Ribonucleases"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Enzyme-Linked Immunosorbent Assay"]},{"majorTopic_YN":["N"],"descriptorName":["Immunohistochemistry"]},{"majorTopic_YN":["N"],"descriptorName":["Polymerase Chain Reaction"]}]}],"chemicalList":[{"chemical":[{"name":["Ribonucleases"],"registryNumber":["EC 3.1.-"]},{"name":["Ribonuclease 7"],"registryNumber":["EC 3.1.27.-"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2712763?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2712763"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=19641608"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=19641608&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0006424"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["31"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2009-11-10"],"dateOfCreation":["2009-07-30"],"dateOfRevision":["2013-06-02"],"electronicPublicationDate":["2009-07-29"],"firstPublicationDate":["2009-07-29"],"cprojectID":"PMC2712763"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSYJ9hGurOzZIXr","_score":1,"_source":{"id":["24198941"],"source":["MED"],"pmid":["24198941"],"pmcid":["PMC3810876"],"doi":["10.1002/ece3.747"],"title":["Massive structural and compositional changes over two decades in forest fragments near Kampala, Uganda."],"authorString":["Bulafu C, Baranga D, Mucunguzi P, Telford RJ, Vandvik V."],"authorList":[{"author":[{"fullName":["Bulafu C"],"firstName":["C"],"lastName":["Bulafu"],"initials":["C"]},{"fullName":["Baranga D"],"firstName":["D"],"lastName":["Baranga"],"initials":["D"]},{"fullName":["Mucunguzi P"],"firstName":["P"],"lastName":["Mucunguzi"],"initials":["P"]},{"fullName":["Telford RJ"],"firstName":["R J"],"lastName":["Telford"],"initials":["RJ"]},{"fullName":["Vandvik V"],"firstName":["V"],"lastName":["Vandvik"],"initials":["V"]}]}],"journalInfo":[{"issue":["11"],"volume":["3"],"journalIssueId":["2091258"],"dateOfPublication":["2013 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2013"],"printPublicationDate":["2013-10-01"],"journal":[{"title":["Ecology and evolution"],"ISOAbbreviation":["Ecol Evol"],"medlineAbbreviation":["Ecol Evol"],"NLMid":["101566408"],"ESSN":["2045-7758"]}]}],"pubYear":["2013"],"pageInfo":["3804-3823"],"abstractText":["Private forests harbor considerable biodiversity, however, they are under greater threat than reserved areas, particularly from urbanization, agriculture, and intense exploitation for timber and fuel wood. The extent to which they may act as habitats for biodiversity and how level of protection impacts trends in biodiversity and forest structure over time remain underresearched. We contribute to filling this research gap by resampling a unique data set, a detailed survey from 1990 of 22 forests fragments of different ownership status and level of protection near Kampala, Uganda. Eleven of the 22 fragments were lost over 20 years, and six of the remnants reduced in size. Forest structure and composition also showed dramatic changes, with six of the remnant fragments showing high temporal species turnover. Species richness increased in four of the remaining forests over the resample period. Forest ownership affected the fate of the forests, with higher loss in privately owned forests. Our study demonstrates that ownership affects the fate of forest fragments, with private forests having both higher rates of area loss, and of structural and compositional change within the remaining fragments. Still, the private forests contribute to the total forest area, and they harbor biodiversity including IUCN \"vulnerable\" and \"endangered\" species. This indicates the conservation value of the fragments and suggests that they should be taken into account in forest conservation and restoration. "],"affiliation":["Department of Biology, School of Natural Sciences, Makerere University P. O. Box 7062, Kampala, Uganda."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Biodiversity","Conservation","turnover","Ownership","Resampling","Redd","Red-listed Species"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3810876?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3810876"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24198941"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24198941&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1002/ece3.747"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-11-07"],"dateOfCreation":["2013-11-07"],"dateOfRevision":["2013-11-12"],"electronicPublicationDate":["2013-09-12"],"firstPublicationDate":["2013-09-12"],"cprojectID":"PMC3810876"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSfhOUS2orL1IN8","_score":1,"_source":{"id":["24058915"],"source":["MED"],"pmid":["24058915"],"pmcid":["PMC3766601"],"doi":["10.1155/2013/838491"],"title":["Possible future monoclonal antibody (mAb)-based therapy against arbovirus infections."],"authorString":["Sautto G, Mancini N, Gorini G, Clementi M, Burioni R."],"authorList":[{"author":[{"fullName":["Sautto G"],"firstName":["Giuseppe"],"lastName":["Sautto"],"initials":["G"],"authorId":[{"_":"0000-0002-0273-2235","$":{"type":"ORCID"}}],"affiliation":["Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, 20132 Milano, Italy."]},{"fullName":["Mancini N"],"firstName":["Nicasio"],"lastName":["Mancini"],"initials":["N"],"authorId":[{"_":"0000-0003-0637-0910","$":{"type":"ORCID"}}]},{"fullName":["Gorini G"],"firstName":["Giacomo"],"lastName":["Gorini"],"initials":["G"]},{"fullName":["Clementi M"],"firstName":["Massimo"],"lastName":["Clementi"],"initials":["M"]},{"fullName":["Burioni R"],"firstName":["Roberto"],"lastName":["Burioni"],"initials":["R"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0273-2235","$":{"type":"ORCID"}},{"_":"0000-0003-0637-0910","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["2013"],"journalIssueId":["2019450"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["BioMed research international"],"ISOAbbreviation":["Biomed Res Int"],"medlineAbbreviation":["Biomed Res Int"],"NLMid":["101600173"],"ISSN":["2314-6133"],"ESSN":["2314-6141"]}]}],"pubYear":["2013"],"pageInfo":["838491"],"abstractText":["More than 150 arboviruses belonging to different families are known to infect humans, causing endemic infections as well as epidemic outbreaks. Effective vaccines to limit the occurrence of some of these infections have been licensed, while for the others several new immunogens are under development mostly for their improvements concerning safety and effectiveness profiles. On the other hand, specific and effective antiviral drugs are not yet available, posing an urgent medical need in particular for emergency cases. Neutralizing monoclonal antibodies (mAbs) have been demonstrated to be effective in the treatment of several infectious diseases as well as in preliminary in vitro and in vivo models of arbovirus-related infections. Given their specific antiviral activity as well-tolerated molecules with limited side effects, mAbs could represent a new therapeutic approach for the development of an effective treatment, as well as useful tools in the study of the host-virus interplay and in the development of more effective immunogens. However, before their use as candidate therapeutics, possible hurdles (e.g., Ab-dependent enhancement of infection, occurrence of viral escape variants) must be carefully evaluated. In this review are described the main arboviruses infecting humans and candidate mAbs to be possibly used in a future passive immunotherapy."],"affiliation":["Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, 20132 Milano, Italy."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Arbovirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DT"],"qualifierName":["drug therapy"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Monoclonal"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TU"],"qualifierName":["therapeutic use"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TU"],"qualifierName":["therapeutic use"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["Antibodies, Monoclonal"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3766601?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3766601"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24058915"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24058915&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2013/838491"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-04-15"],"dateOfCreation":["2013-09-23"],"electronicPublicationDate":["2013-08-22"],"firstPublicationDate":["2013-08-22"],"cprojectID":"PMC3766601"}}
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Besides yellow fever virus, a landmark case in the history of virology, members of the Flavivirus genus, such as West Nile virus and dengue virus, are increasingly gaining attention due to their re-emergence and incidence in different areas of the world. Additional environmental and demographic considerations suggest that novel or known flaviviruses will continue to emerge in the future. Nevertheless, up to few years ago flaviviruses were considered low interest candidates for drug design. At the start of the European Union VIZIER Project, in 2004, just two crystal structures of protein domains from the flaviviral replication machinery were known. Such pioneering studies, however, indicated the flaviviral replication complex as a promising target for the development of antiviral compounds. Here we review structural and functional aspects emerging from the characterization of two main components (NS3 and NS5 proteins) of the flavivirus replication complex. Most of the reviewed results were achieved within the European Union VIZIER Project, and cover topics that span from viral genomics to structural biology and inhibition mechanisms. The ultimate aim of the reported approaches is to shed light on the design and development of antiviral drug leads."],"affiliation":["Department of Biomolecular Sciences and Biotechnology, University of Milano, Via Celoria 26, 20133 Milano, Italy."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"grantId":["CEH010021"],"agency":["Natural Environment Research Council"],"orderIn":["0"]},{"grantId":["ceh010021"],"agency":["Natural Environment Research Council"],"orderIn":["0"]},{"grantId":["G0701506"],"agency":["Medical Research Council"],"orderIn":["0"]},{"grantId":["G0700762"],"agency":["Medical Research Council"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["N"]},{"abbreviation":["EN"],"qualifierName":["enzymology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Enzymes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Nonstructural Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["AI"],"qualifierName":["antagonists & inhibitors"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Enzyme Inhibitors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antiviral Agents"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["N"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Virus Replication"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Drug Design"]},{"majorTopic_YN":["N"],"descriptorName":["Biomedical Research"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["OG"],"qualifierName":["organization & administration"],"majorTopic_YN":["N"]},{"abbreviation":["TD"],"qualifierName":["trends"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["European Union"]}]}],"chemicalList":[{"chemical":[{"name":["Enzymes"],"registryNumber":["0"]},{"name":["Antiviral Agents"],"registryNumber":["0"]},{"name":["Enzyme Inhibitors"],"registryNumber":["0"]},{"name":["Viral Nonstructural Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3918146"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=19945487"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.antiviral.2009.11.009"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["58"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCompletion":["2010-10-19"],"dateOfCreation":["2010-07-20"],"dateOfRevision":["2014-05-25"],"electronicPublicationDate":["2009-11-27"],"firstPublicationDate":["2009-11-27"],"cprojectID":"PMC3918146"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqST_8VqgcuJwvfq","_score":1,"_source":{"id":["19788800"],"source":["MED"],"pmid":["19788800"],"pmcid":["PMC2819875"],"doi":["10.3201/eid1509.090442"],"title":["Zika virus outside Africa."],"authorString":["Hayes EB."],"authorList":[{"author":[{"fullName":["Hayes EB"],"firstName":["Edward B"],"lastName":["Hayes"],"initials":["EB"],"affiliation":["Barcelona Centre for International Health Research, Barcelona, Spain. ned.hayes@cresib.cat"]}]}],"journalInfo":[{"issue":["9"],"volume":["15"],"journalIssueId":["1653601"],"dateOfPublication":["2009 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2009"],"printPublicationDate":["2009-09-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2009"],"pageInfo":["1347-1350"],"abstractText":["Zika virus (ZIKV) is a flavivirus related to yellow fever, dengue, West Nile, and Japanese encephalitis viruses. In 2007 ZIKV caused an outbreak of relatively mild disease characterized by rash, arthralgia, and conjunctivitis on Yap Island in the southwestern Pacific Ocean. This was the first time that ZIKV was detected outside of Africa and Asia. The history, transmission dynamics, virology, and clinical manifestations of ZIKV disease are discussed, along with the possibility for diagnostic confusion between ZIKV illness and dengue.The emergence of ZIKV outside of its previously known geographic range should prompt awareness of the potential for ZIKV to spread to other Pacific islands and the Americas."],"affiliation":["Barcelona Centre for International Health Research, Barcelona, Spain. ned.hayes@cresib.cat"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Communicable Diseases, Emerging"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["PP"],"qualifierName":["physiopathology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Antibodies, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["BL"],"qualifierName":["blood"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Child"]},{"majorTopic_YN":["N"],"descriptorName":["Child, Preschool"]},{"majorTopic_YN":["N"],"descriptorName":["Infant"]},{"majorTopic_YN":["N"],"descriptorName":["Micronesia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PP"],"qualifierName":["physiopathology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Antibodies, Viral"],"registryNumber":["0"]},{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2819875?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2819875"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=19788800&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=19788800"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1509.090442"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["50"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2010-01-05"],"dateOfCreation":["2009-09-30"],"dateOfRevision":["2016-02-05"],"firstPublicationDate":["2009-09-01"],"cprojectID":"PMC2819875"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqww_8VqgcuJwvgm","_score":1,"_source":{"id":["25844007"],"source":["MED"],"pmid":["25844007"],"pmcid":["PMC4375606"],"doi":["10.1016/j.landusepol.2014.01.015"],"title":["Land system change in Italy from 1884 to 2007: Analysing the North-South divergence on the basis of an integrated indicator framework."],"authorString":["Niedertscheider M, Erb K."],"authorList":[{"author":[{"fullName":["Niedertscheider M"],"firstName":["Maria"],"lastName":["Niedertscheider"],"initials":["M"],"affiliation":["Institute of Social Ecology, Vienna, Alpen-Adria Universität, Schottenfeldgasse 29, 1070 Vienna, Austria."]},{"fullName":["Erb K"],"firstName":["Karlheinz"],"lastName":["Erb"],"initials":["K"],"authorId":[{"_":"0000-0002-8335-4159","$":{"type":"ORCID"}}],"affiliation":["Institute of Social Ecology, Vienna, Alpen-Adria Universität, Schottenfeldgasse 29, 1070 Vienna, Austria."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8335-4159","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["39"],"journalIssueId":["2270724"],"dateOfPublication":["2014 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2014"],"printPublicationDate":["2014-07-01"],"journal":[{"title":["Land use policy"],"ISOAbbreviation":["Land use policy"],"medlineAbbreviation":["Land use policy"],"NLMid":["101085960"],"ISSN":["0264-8377"],"ESSN":["1873-5754"]}]}],"pubYear":["2014"],"pageInfo":["366-375"],"abstractText":["Over the past centuries, land systems in Italy experienced fundamental shifts, owing to the availability of new energy forms, population surges, and technological progress. The 20th century was characterized by massive productivity increases, accompanied by gradual land abandonment and the return of forest land. We here analyze 120 years of land system change in Italy, applying the human appropriation of net primary production (HANPP) framework, a metric for socio-economic pressures on terrestrial ecosystems. HANPP allows integrating ecological with societal perspectives, by systematically quantifying (a) biomass harvest and (b) the difference between potential productivity of ecosystems and current productivity induced by land use processes, such as land conversion, or land degradation. Besides assessing national trends we calculated HANPP separately for the Italian North and South between 1934 and 2007, in order to scrutinize if high regional discrepancies in terms of natural and socio-economic preconditions translate into diverging land system trajectories. Our results show that national HANPP has been declining from 78% of natural productivity before WWII to 56% in 2007, indicating a declining land -use induced pressure on biomass flows over time. Simultaneously, biomass harvest increased by around 26% due to agricultural intensification, despite shrinking croplands. Although we found a significant difference between the Northern and Southern region in the absolute levels of several land use indicators related to biomass appropriation, the overarching trends of land system change were remarkably similar in both regions. This suggests that underlying drivers of land system change, such as policies aimed at land-use intensification and structural change were equally dominating land system trajectories in the North and South of Italy, not withstanding their socio-ecological divergences."],"affiliation":["Institute of Social Ecology, Vienna, Alpen-Adria Universität, Schottenfeldgasse 29, 1070 Vienna, Austria."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["263522"],"agency":["European Research Council"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Italy","Long-term Land System Change","Hanpp","Socio-ecological Perspective","Land Use Intensification"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4375606"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.landusepol.2014.01.015"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2015-04-06"],"dateOfRevision":["2015-04-08"],"firstPublicationDate":["2014-07-01"],"cprojectID":"PMC4375606"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqz4J9hGurOzZIYu","_score":1,"_source":{"id":["26683460"],"source":["MED"],"pmid":["26683460"],"pmcid":["PMC4686165"],"doi":["10.1371/journal.pone.0145311"],"title":["Seasonal Synchronization of Diapause Phases in Aedes albopictus (Diptera: Culicidae)."],"authorString":["Lacour G, Chanaud L, L'Ambert G, Hance T."],"authorList":[{"author":[{"fullName":["Lacour G"],"firstName":["Guillaume"],"lastName":["Lacour"],"initials":["G"],"authorId":[{"_":"0000-0002-9588-6064","$":{"type":"ORCID"}}],"affiliation":["Centre de Recherche sur la Biodiversité, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgique."]},{"fullName":["Chanaud L"],"firstName":["Lionel"],"lastName":["Chanaud"],"initials":["L"],"affiliation":["EID Méditerranée, Montpellier, France."]},{"fullName":["L'Ambert G"],"firstName":["Grégory"],"lastName":["L'Ambert"],"initials":["G"],"affiliation":["EID Méditerranée, Montpellier, France."]},{"fullName":["Hance T"],"firstName":["Thierry"],"lastName":["Hance"],"initials":["T"],"affiliation":["Centre de Recherche sur la Biodiversité, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgique."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-9588-6064","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["12"],"volume":["10"],"journalIssueId":["2347465"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0145311"],"abstractText":["In temperate areas, population dynamics of the invasive Asian tiger mosquito Aedes albopictus are strongly affected by winter. The work we present here analyzes the adaptive synchronization of the diapause process in the wintry generation of A. albopictus, where the egg stage is exposed to adverse winter conditions. The seasonal pattern of egg laying activity of a French Mediterranean population of the Asian tiger mosquito was monitored weekly for 2 years with ovitraps. The field diapause incidence and the critical photoperiod (CPP, i.e. the maternal day length inducing diapause in 50% of the eggs), were determined by hatching experiments on the collected eggs. The period of diapause termination was estimated by a field survey of the first hatchings for both years. The CPP is equal to 13.5 hours of light and occurs in the field on the 25th of August. Thus, it is on September 11th, 17 days after the CPP, that 50% of the eggs are in a prediapause stage in the field. The egg diapause rate increases rapidly during September, whereas the mean number of eggs laid decreases sharply after mid-September. Surprisingly, after having reached a peak of 95% at the end of September, from mid-October the diapause incidence declined and stayed below 50%. Indeed, both years the diapause initiates before the rapid decrease of the environmental temperature. This leaves a sufficient period of time to the complete development of one generation of A. albopictus with effective induction of diapause in the laid eggs. The very first larvae hatched were sampled both years in the first half of March. With 20 to 26 weeks in the egg stage and about 7 weeks in the larval stages, the first annual generation spends a long time in immature stages. On a practical point of view, this long development time represents a wide window for eggs and larvae control in early spring."],"affiliation":["Centre de Recherche sur la Biodiversité, Earth and Life Institute, Université catholique de Louvain, Louvain-la-Neuve, Belgique."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Seasons"]},{"majorTopic_YN":["N"],"descriptorName":["Larva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GD"],"qualifierName":["growth & development"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Oviposition"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4686165?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4686165"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0145311"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-22"],"dateOfCreation":["2015-12-19"],"dateOfRevision":["2016-01-08"],"electronicPublicationDate":["2015-12-18"],"firstPublicationDate":["2015-12-18"],"cprojectID":"PMC4686165"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq0qJ9hGurOzZIYw","_score":1,"_source":{"id":["26823941"],"source":["MED"],"pmid":["26823941"],"pmcid":["PMC4729430"],"doi":["10.5811/westjem.2016.1.29767"],"title":["Frozen Funding on Firearm Research: \"Doing Nothing Is No Longer an Acceptable Solution\"."],"authorString":["Betz ME, Ranney ML, Wintemute GJ."],"authorList":[{"author":[{"fullName":["Betz ME"],"firstName":["Marian E"],"lastName":["Betz"],"initials":["ME"],"affiliation":["University of Colorado School of Medicine, Department of Emergency Medicine, Denver, Colorado."]},{"fullName":["Ranney ML"],"firstName":["Megan L"],"lastName":["Ranney"],"initials":["ML"],"affiliation":["Rhode Island Hospital/Alpert Medical School, Brown University, Department of Emergency Medicine, Providence, Rhode Island."]},{"fullName":["Wintemute GJ"],"firstName":["Garen J"],"lastName":["Wintemute"],"initials":["GJ"],"affiliation":["University of California, Davis, Violence Prevention Research Program, Department of Emergency Medicine; Sacramento, California."]}]}],"journalInfo":[{"issue":["1"],"volume":["17"],"journalIssueId":["2368372"],"dateOfPublication":["2016 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["The western journal of emergency medicine"],"ISOAbbreviation":["West J Emerg Med"],"medlineAbbreviation":["West J Emerg Med"],"NLMid":["101476450"],"ISSN":["1936-900X"],"ESSN":["1936-9018"]}]}],"pubYear":["2016"],"pageInfo":["91-93"],"affiliation":["University of Colorado School of Medicine, Department of Emergency Medicine, Denver, Colorado."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4729430?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4729430"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.5811/westjem.2016.1.29767"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-01-29"],"dateOfRevision":["2016-02-02"],"electronicPublicationDate":["2016-01-14"],"firstPublicationDate":["2016-01-14"],"cprojectID":"PMC4729430"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq3rhOUS2orL1IO7","_score":1,"_source":{"id":["26355096"],"source":["MED"],"pmid":["26355096"],"pmcid":["PMC4645344"],"doi":["10.1128/jvi.01543-15"],"title":["Ecuador Paraiso Escondido Virus, a New Flavivirus Isolated from New World Sand Flies in Ecuador, Is the First Representative of a Novel Clade in the Genus Flavivirus."],"authorString":["Alkan C, Zapata S, Bichaud L, Moureau G, Lemey P, Firth AE, Gritsun TS, Gould EA, de Lamballerie X, Depaquit J, Charrel RN."],"authorList":[{"author":[{"fullName":["Alkan C"],"firstName":["Cigdem"],"lastName":["Alkan"],"initials":["C"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France cgdmalkan@gmail.com remi.charrel@univ-amu.fr."]},{"fullName":["Zapata S"],"firstName":["Sonia"],"lastName":["Zapata"],"initials":["S"],"affiliation":["Instituto de Microbiología, Universidad San Francisco de Quito, Quito, Ecuador."]},{"fullName":["Bichaud L"],"firstName":["Laurence"],"lastName":["Bichaud"],"initials":["L"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Moureau G"],"firstName":["Grégory"],"lastName":["Moureau"],"initials":["G"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Lemey P"],"firstName":["Philippe"],"lastName":["Lemey"],"initials":["P"],"affiliation":["Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium."]},{"fullName":["Firth AE"],"firstName":["Andrew E"],"lastName":["Firth"],"initials":["AE"],"authorId":[{"_":"0000-0002-7986-9520","$":{"type":"ORCID"}}],"affiliation":["Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom."]},{"fullName":["Gritsun TS"],"firstName":["Tamara S"],"lastName":["Gritsun"],"initials":["TS"],"affiliation":["School of Biological Sciences, University of Reading, Whiteknights, Reading, United Kingdom."]},{"fullName":["Gould EA"],"firstName":["Ernest A"],"lastName":["Gould"],"initials":["EA"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["de Lamballerie X"],"firstName":["Xavier"],"lastName":["de Lamballerie"],"initials":["X"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France."]},{"fullName":["Depaquit J"],"firstName":["Jérôme"],"lastName":["Depaquit"],"initials":["J"],"affiliation":["Université de Reims Champagne-Ardenne, ANSES, EA 4688 USC VECPAR Transmission Vectorielle et Épidémiosurveillance de Maladies Parasitaires, SFR Capsanté, Reims, France."]},{"fullName":["Charrel RN"],"firstName":["Rémi N"],"lastName":["Charrel"],"initials":["RN"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France cgdmalkan@gmail.com remi.charrel@univ-amu.fr."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7986-9520","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["23"],"volume":["89"],"journalIssueId":["2341043"],"dateOfPublication":["2015 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2015"],"printPublicationDate":["2015-12-01"],"journal":[{"title":["Journal of virology"],"ISOAbbreviation":["J. Virol."],"medlineAbbreviation":["J Virol"],"NLMid":["0113724"],"ISSN":["0022-538X"],"ESSN":["1098-5514"]}]}],"pubYear":["2015"],"pageInfo":["11773-11785"],"abstractText":["A new flavivirus, Ecuador Paraiso Escondido virus (EPEV), named after the village where it was discovered, was isolated from sand flies (Psathyromyia abonnenci, formerly Lutzomyia abonnenci) that are unique to the New World. This represents the first sand fly-borne flavivirus identified in the New World. EPEV exhibited a typical flavivirus genome organization. Nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. Phylogenetic analysis of the complete coding sequence showed that EPEV represents a distinct clade which diverged from a lineage that was ancestral to the nonvectored flaviviruses Entebbe bat virus, Yokose virus, and Sokoluk virus and also the Aedes-associated mosquito-borne flaviviruses, which include yellow fever virus, Sepik virus, Saboya virus, and others. EPEV replicated in C6/36 mosquito cells, yielding high infectious titers, but failed to reproduce either in vertebrate cell lines (Vero, BHK, SW13, and XTC cells) or in suckling mouse brains. This surprising result, which appears to eliminate an association with vertebrate hosts in the life cycle of EPEV, is discussed in the context of the evolutionary origins of EPEV in the New World.The flaviviruses are rarely (if ever) vectored by sand fly species, at least in the Old World. We have identified the first representative of a sand fly-associated flavivirus, Ecuador Paraiso Escondido virus (EPEV), in the New World. EPEV constitutes a novel clade according to current knowledge of the flaviviruses. Phylogenetic analysis of the virus genome showed that EPEV roots the Aedes-associated mosquito-borne flaviviruses, including yellow fever virus. In light of this new discovery, the New World origin of EPEV is discussed together with that of the other flaviviruses."],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France IHU Mediterranée Infection, APHM Public Hospitals of Marseille, Marseille, France cgdmalkan@gmail.com remi.charrel@univ-amu.fr."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Brain"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cell Line"]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Psychodidae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Bayes Theorem"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Alignment"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["Y"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Virus Replication"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Species Specificity"]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acid Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Base Sequence"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Homology"]},{"majorTopic_YN":["N"],"descriptorName":["Genome, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Models, Genetic"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Ecuador"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4645344?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4645344"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/JVI.01543-15"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["refseq"," gen"," "]}],"dateOfCompletion":["2016-03-01"],"dateOfCreation":["2015-11-06"],"dateOfRevision":["2015-12-14"],"electronicPublicationDate":["2015-09-09"],"firstPublicationDate":["2015-09-09"],"cprojectID":"PMC4645344"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrBDhOUS2orL1IPV","_score":1,"_source":{"id":["26966643"],"source":["MED"],"pmid":["26966643"],"pmcid":["PMC4782685"],"doi":["10.7717/peerj.1613"],"title":["Samples and data accessibility in research biobanks: an explorative survey."],"authorString":["Capocasa M, Anagnostou P, D'Abramo F, Matteucci G, Dominici V, Destro Bisol G, Rufo F."],"authorList":[{"author":[{"fullName":["Capocasa M"],"firstName":["Marco"],"lastName":["Capocasa"],"initials":["M"],"affiliation":["Istituto Italiano di Antropologia , Rome , Italy."]},{"fullName":["Anagnostou P"],"firstName":["Paolo"],"lastName":["Anagnostou"],"initials":["P"],"affiliation":["Istituto Italiano di Antropologia, Rome, Italy; Department of Environmental Biology, Sapienza University of Rome, Rome, Italy."]},{"fullName":["D'Abramo F"],"firstName":["Flavio"],"lastName":["D'Abramo"],"initials":["F"],"affiliation":["Charité Comprehensive Cancer Center , Berlin , Germany."]},{"fullName":["Matteucci G"],"firstName":["Giulia"],"lastName":["Matteucci"],"initials":["G"],"affiliation":["Istituto Italiano di Antropologia , Rome , Italy."]},{"fullName":["Dominici V"],"firstName":["Valentina"],"lastName":["Dominici"],"initials":["V"],"affiliation":["Istituto Italiano di Antropologia, Rome, Italy; Department of Environmental Biology, Sapienza University of Rome, Rome, Italy."]},{"fullName":["Destro Bisol G"],"firstName":["Giovanni"],"lastName":["Destro Bisol"],"initials":["G"],"affiliation":["Istituto Italiano di Antropologia, Rome, Italy; Department of Environmental Biology, Sapienza University of Rome, Rome, Italy."]},{"fullName":["Rufo F"],"firstName":["Fabrizio"],"lastName":["Rufo"],"initials":["F"],"affiliation":["Istituto Italiano di Antropologia, Rome, Italy; Department of Environmental Biology, Sapienza University of Rome, Rome, Italy."]}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2370428"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PeerJ"],"ISOAbbreviation":["PeerJ"],"medlineAbbreviation":["PeerJ"],"NLMid":["101603425"],"ESSN":["2167-8359"]}]}],"pubYear":["2016"],"pageInfo":["e1613"],"abstractText":["Biobanks, which contain human biological samples and/or data, provide a crucial contribution to the progress of biomedical research. However, the effective and efficient use of biobank resources depends on their accessibility. In fact, making bio-resources promptly accessible to everybody may increase the benefits for society. Furthermore, optimizing their use and ensuring their quality will promote scientific creativity and, in general, contribute to the progress of bio-medical research. Although this has become a rather common belief, several laboratories are still secretive and continue to withhold samples and data. In this study, we conducted a questionnaire-based survey in order to investigate sample and data accessibility in research biobanks operating all over the world. The survey involved a total of 46 biobanks. Most of them gave permission to access their samples (95.7%) and data (85.4%), but free and unconditioned accessibility seemed not to be common practice. The analysis of the guidelines regarding the accessibility to resources of the biobanks that responded to the survey highlights three issues: (i) the request for applicants to explain what they would like to do with the resources requested; (ii) the role of funding, public or private, in the establishment of fruitful collaborations between biobanks and research labs; (iii) the request of co-authorship in order to give access to their data. These results suggest that economic and academic aspects are involved in determining the extent of sample and data sharing stored in biobanks. As a second step of this study, we investigated the reasons behind the high diversity of requirements to access biobank resources. The analysis of informative answers suggested that the different modalities of resource accessibility seem to be largely influenced by both social context and legislation of the countries where the biobanks operate."],"affiliation":["Istituto Italiano di Antropologia , Rome , Italy."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["data sharing","Human Subjects","Research Ethics","Biorepository","Open Science"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4782685?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4782685"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7717/peerj.1613"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-03-11"],"dateOfCreation":["2016-03-11"],"dateOfRevision":["2016-03-13"],"electronicPublicationDate":["2016-02-25"],"firstPublicationDate":["2016-02-25"],"cprojectID":"PMC4782685"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrDLJ9hGurOzZIZO","_score":1,"_source":{"id":["27007567"],"source":["MED"],"pmid":["27007567"],"pmcid":["PMC4804561"],"doi":["10.1590/s1678-9946201658024"],"title":["INTRODUCTION AND TRANSMISSION OF ZIKA VIRUS IN BRAZIL: NEW CHALLENGES FOR THE AMERICAS."],"authorString":["Freitas AR, Angerami RN, von Zuben AP, Donalisio MR."],"authorList":[{"author":[{"fullName":["Freitas AR"],"firstName":["André Ricardo Ribas"],"lastName":["Freitas"],"initials":["AR"],"affiliation":["Departamento de Medicina Social, Faculdade de Medicina São Leopoldo Mandic, Campinas, SP, Brasil, arrfreitas2010@gmail.com."]},{"fullName":["Angerami RN"],"firstName":["Rodrigo Nogueira"],"lastName":["Angerami"],"initials":["RN"],"affiliation":["Hospital das Clínicas, Departamento de Infectologia, Universidade Estadual de Campinas, Campinas, SP, Brasil, rodrigo.angerami@gmail.com."]},{"fullName":["von Zuben AP"],"firstName":["Andrea Paula Bruno"],"lastName":["von Zuben"],"initials":["AP"],"affiliation":["Departamento de Vigilância à Saúde, Campinas, SP, Brasil, andreabrunovonzuben@gmail.com."]},{"fullName":["Donalisio MR"],"firstName":["Maria Rita"],"lastName":["Donalisio"],"initials":["MR"],"affiliation":["Departamento de Saúde Coletiva, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil, rita.donalisio@gmail.com."]}]}],"journalInfo":[{"volume":["58"],"journalIssueId":["2390260"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Revista do Instituto de Medicina Tropical de Sao Paulo"],"ISOAbbreviation":["Rev. Inst. Med. Trop. Sao Paulo"],"medlineAbbreviation":["Rev Inst Med Trop Sao Paulo"],"NLMid":["7507484"],"ISSN":["0036-4665"],"ESSN":["1678-9946"]}]}],"pubYear":["2016"],"pageInfo":["24"],"affiliation":["Departamento de Medicina Social, Faculdade de Medicina São Leopoldo Mandic, Campinas, SP, Brasil, arrfreitas2010@gmail.com."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Letter"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4804561?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4804561"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/S1678-9946201658024"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-16"],"dateOfCreation":["2016-03-24"],"dateOfRevision":["2016-04-08"],"electronicPublicationDate":["2016-03-22"],"firstPublicationDate":["2016-03-22"],"cprojectID":"PMC4804561"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrCHJ9hGurOzZIZL","_score":1,"_source":{"id":["PMC4800905"],"source":["PMC"],"pmcid":["PMC4800905"],"title":["WHO STATEMENT ON THE FIRST MEETING OF THE INTERNATIONAL HEALTH REGULATIONS (2005) (IHR 2005) EMERGENCY COMMITTEE ON ZIKA VIRUS AND OBSERVED INCREASE IN NEUROLOGICAL DISORDERS AND NEONATAL MALFORMATIONS."],"journalInfo":[{"issue":["3"],"volume":["37"],"journalIssueId":["2378001"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Saudi medical journal"],"ISOAbbreviation":["Saudi Med J"],"medlineAbbreviation":["Saudi Med J"],"NLMid":["7909441"],"ISSN":["0379-5284"]}]}],"pubYear":["2016"],"pageInfo":["332-333"],"language":["eng"],"pubModel":["Undetermined"],"pubTypeList":[{"pubType":["News"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4800905"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.org/articles/PMC4800905/?tool=EBI"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-03"],"dateOfRevision":["2016-04-03"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4800905"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrEg_8VqgcuJwvhX","_score":1,"_source":{"id":["26931565"],"source":["MED"],"pmid":["26931565"],"pmcid":["PMC4774189"],"doi":["10.1186/s12985-016-0494-6"],"title":["Inhibition of protein kinase C promotes dengue virus replication."],"authorString":["Noppakunmongkolchai W, Poyomtip T, Jittawuttipoka T, Luplertlop N, Sakuntabhai A, Chimnaronk S, Jirawatnotai S, Tohtong R."],"authorList":[{"author":[{"fullName":["Noppakunmongkolchai W"],"firstName":["Warobon"],"lastName":["Noppakunmongkolchai"],"initials":["W"],"affiliation":["Department of Biochemistry, Faculty of Sciences, Mahidol University, Bangkok, Thailand. warobon.nop@mtec.or.th."]},{"fullName":["Poyomtip T"],"firstName":["Teera"],"lastName":["Poyomtip"],"initials":["T"],"affiliation":["Department of Microbiology, Faculty of Sciences, Mahidol University, Bangkok, Thailand. teera.poy@student.mahidol.ac.th."]},{"fullName":["Jittawuttipoka T"],"firstName":["Thichakorn"],"lastName":["Jittawuttipoka"],"initials":["T"],"affiliation":["Department of Microbiology, Faculty of Sciences, Mahidol University, Bangkok, Thailand. thicha_korn@hotmail.com."]},{"fullName":["Luplertlop N"],"firstName":["Natthanej"],"lastName":["Luplertlop"],"initials":["N"],"affiliation":["Department of Microbiology and Immunology, Faculty of Tropical Medicine, Thailand Functional Genetics of Infectious Diseases Unit, Institute, Mahidol University, Bangkok, Thailand. natthanej.lup@mahidol.ac.th."]},{"fullName":["Sakuntabhai A"],"firstName":["Anavaj"],"lastName":["Sakuntabhai"],"initials":["A"],"affiliation":["Systems Biology of Diseases Research Unit, Faculty of Science, Mahidol University, Bangkok, Thailand. anavaj.sakuntabhai@pasteur.fr."]},{"fullName":["Chimnaronk S"],"firstName":["Sarin"],"lastName":["Chimnaronk"],"initials":["S"],"affiliation":["Systems Biology of Diseases Research Unit, Faculty of Science, Mahidol University, Bangkok, Thailand. sarin.chi@mahidol.ac.th."]},{"fullName":["Jirawatnotai S"],"firstName":["Siwanon"],"lastName":["Jirawatnotai"],"initials":["S"],"affiliation":["Laboratory for Systems Pharmacology, Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand. siwanon.jir@mahidol.ac.th."]},{"fullName":["Tohtong R"],"firstName":["Rutaiwan"],"lastName":["Tohtong"],"initials":["R"],"affiliation":["Department of Biochemistry, Faculty of Sciences, Mahidol University, Bangkok, Thailand. rutaiwan.toh@mahidol.ac.th."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2401989"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["35"],"abstractText":["Dengue virus (DENV) is a member of the Flaviviridae family, transmitted to human via mosquito. DENV infection is common in tropical areas and occasionally causes life-threatening symptoms. DENV contains a relatively short positive-stranded RNA genome, which encodes ten viral proteins. Thus, the viral life cycle is necessarily rely on or regulated by host factors.In silico analyses in conjunction with in vitro kinase assay were used to study kinases that potentially phosphorylate DENV NS5. Potential kinase was inhibited or activated by a specific inhibitor (or siRNA), or an activator. Results of the inhibition and activation on viral entry/replication and host cell survival were examined.Our in silico analyses indicated that the non-structural protein 5 (NS5), especially the RNA-dependent RNA polymerase (RdRp) domain, contains conserved phosphorylation sites for protein kinase C (PKC). Phosphorylation of NS5 RdRp was further verified by PKC in vitro kinase assay. Inhibitions of PKC by a PKC-specific chemical inhibitor or siRNA suppressed NS5 phosphorylation in vivo, increased viral replication and reduced viability of the DENV-infected cells. In contrary, activation of PKC effectively suppressed intracellular viral number.These results indicated that PKC may act as a restricting mechanism that modulates the DENV replication and represses the viral outburst in the host cells."],"affiliation":["Department of Biochemistry, Faculty of Sciences, Mahidol University, Bangkok, Thailand. warobon.nop@mtec.or.th."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4774189?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4774189"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0494-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCreation":["2016-03-02"],"dateOfRevision":["2016-03-07"],"electronicPublicationDate":["2016-03-01"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4774189"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrIlJ9hGurOzZIZe","_score":1,"_source":{"id":["27099632"],"source":["MED"],"pmid":["27099632"],"pmcid":["PMC4837584"],"doi":["10.1186/s13039-016-0240-1"],"title":["The mitotic spindle: linking teratogenic effects of Zika virus with human genetics?"],"authorString":["Bullerdiek J, Dotzauer A, Bauer I."],"authorList":[{"author":[{"fullName":["Bullerdiek J"],"firstName":["Joern"],"lastName":["Bullerdiek"],"initials":["J"],"affiliation":["Centre for Human Genetics, University of Bremen, Leobener Str. ZHG, D-28359 Bremen, Germany ; Institute of Medical Genetics, University Rostock Medical Center, Ernst-Heydemann-Strasse 8, D-18057 Rostock, Germany."]},{"fullName":["Dotzauer A"],"firstName":["Andreas"],"lastName":["Dotzauer"],"initials":["A"],"affiliation":["Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, D-28359 Bremen, Germany."]},{"fullName":["Bauer I"],"firstName":["Ingrid"],"lastName":["Bauer"],"initials":["I"],"affiliation":["Institute of Medical Genetics, University Rostock Medical Center, Ernst-Heydemann-Strasse 8, D-18057 Rostock, Germany."]}]}],"journalInfo":[{"volume":["9"],"journalIssueId":["2370407"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Molecular cytogenetics"],"ISOAbbreviation":["Mol Cytogenet"],"medlineAbbreviation":["Mol Cytogenet"],"NLMid":["101317942"],"ESSN":["1755-8166"]}]}],"pubYear":["2016"],"pageInfo":["32"],"abstractText":["Recently, an association between Zika virus infection and microcephaly/ocular findings was found to be reasonable e.g. because of the demonstration that the virus was found in the brain of a fetus after presumed maternal infection. Although there is no proof yet for a causal relationship, for an appropriate risk calculation efforts are urgently needed to either establish or disprove this assumption.On the basis of inherited syndromes combining microcephaly with ocular findings similar to those associated with Zika infections, we have hypothesized that the impairment of the proper function of the mitotic apparatus is a possible mechanism by which Zika can exert teratogenic effects.A bundle of well-known cytogenetic and molecular-cytogenetic methods (e.g. formation of micronuclei, chromosomal lagging, immunofluorescence of centrosomes) to evaluate proper function, maintenance, and establishment of the mitotic spindle poles can be applied on infected cells. Also, the viral proteins can be tested for their possible interaction with proteins encoded by genes involved in inherited syndromes with microcephaly and ocular findings resembling those in presumed cases of intrauterine ZIKV infection.Once proved, this hypothesis allows for a targeted approach into mechanisms of possible relevance as e.g. if different strains of the virus are implicated in the teratogenic effects to the same or a different extent."],"affiliation":["Centre for Human Genetics, University of Bremen, Leobener Str. ZHG, D-28359 Bremen, Germany ; Institute of Medical Genetics, University Rostock Medical Center, Ernst-Heydemann-Strasse 8, D-18057 Rostock, Germany."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Genetics","Mechanisms","Microcephaly","Syndromes","Phenocopy","Chorioretinopathy","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837584?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837584"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13039-016-0240-1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," omim"," "]}],"dateOfCompletion":["2016-04-21"],"dateOfCreation":["2016-04-21"],"dateOfRevision":["2016-04-23"],"electronicPublicationDate":["2016-04-19"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4837584"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSRJ9hGurOzZIXj","_score":1,"_source":{"id":["18698358"],"source":["MED"],"pmid":["18698358"],"pmcid":["PMC2491585"],"doi":["10.1371/journal.pntd.0000272"],"title":["Conservation and variability of dengue virus proteins: implications for vaccine design."],"authorString":["Khan AM, Miotto O, Nascimento EJ, Srinivasan KN, Heiny AT, Zhang GL, Marques ET, Tan TW, Brusic V, Salmon J, August JT."],"authorList":[{"author":[{"fullName":["Khan AM"],"firstName":["Asif M"],"lastName":["Khan"],"initials":["AM"],"authorId":[{"_":"0000-0001-9202-279X","$":{"type":"ORCID"}}]},{"fullName":["Miotto O"],"firstName":["Olivo"],"lastName":["Miotto"],"initials":["O"],"authorId":[{"_":"0000-0001-8060-6771","$":{"type":"ORCID"}}]},{"fullName":["Nascimento EJ"],"firstName":["Eduardo J M"],"lastName":["Nascimento"],"initials":["EJ"]},{"fullName":["Srinivasan KN"],"firstName":["K N"],"lastName":["Srinivasan"],"initials":["KN"]},{"fullName":["Heiny AT"],"firstName":["A T"],"lastName":["Heiny"],"initials":["AT"]},{"fullName":["Zhang GL"],"firstName":["Guang Lan"],"lastName":["Zhang"],"initials":["GL"]},{"fullName":["Marques ET"],"firstName":["E T"],"lastName":["Marques"],"initials":["ET"]},{"fullName":["Tan TW"],"firstName":["Tin Wee"],"lastName":["Tan"],"initials":["TW"]},{"fullName":["Brusic V"],"firstName":["Vladimir"],"lastName":["Brusic"],"initials":["V"]},{"fullName":["Salmon J"],"firstName":["Jerome"],"lastName":["Salmon"],"initials":["J"]},{"fullName":["August JT"],"firstName":["J Thomas"],"lastName":["August"],"initials":["JT"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9202-279X","$":{"type":"ORCID"}},{"_":"0000-0001-8060-6771","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["2"],"journalIssueId":["1547287"],"dateOfPublication":["2008 "],"monthOfPublication":["0"],"yearOfPublication":["2008"],"printPublicationDate":["2008-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2008"],"pageInfo":["e272"],"abstractText":[" BACKGROUND: Genetic variation and rapid evolution are hallmarks of RNA viruses, the result of high mutation rates in RNA replication and selection of mutants that enhance viral adaptation, including the escape from host immune responses. Variability is uneven across the genome because mutations resulting in a deleterious effect on viral fitness are restricted. RNA viruses are thus marked by protein sites permissive to multiple mutations and sites critical to viral structure-function that are evolutionarily robust and highly conserved. Identification and characterization of the historical dynamics of the conserved sites have relevance to multiple applications, including potential targets for diagnosis, and prophylactic and therapeutic purposes. METHODOLOGY/PRINCIPAL FINDINGS: We describe a large-scale identification and analysis of evolutionarily highly conserved amino acid sequences of the entire dengue virus (DENV) proteome, with a focus on sequences of 9 amino acids or more, and thus immune-relevant as potential T-cell determinants. DENV protein sequence data were collected from the NCBI Entrez protein database in 2005 (9,512 sequences) and again in 2007 (12,404 sequences). Forty-four (44) sequences (pan-DENV sequences), mainly those of nonstructural proteins and representing approximately 15% of the DENV polyprotein length, were identical in 80% or more of all recorded DENV sequences. Of these 44 sequences, 34 ( approximately 77%) were present in >or=95% of sequences of each DENV type, and 27 ( approximately 61%) were conserved in other Flaviviruses. The frequencies of variants of the pan-DENV sequences were low (0 to approximately 5%), as compared to variant frequencies of approximately 60 to approximately 85% in the non pan-DENV sequence regions. We further showed that the majority of the conserved sequences were immunologically relevant: 34 contained numerous predicted human leukocyte antigen (HLA) supertype-restricted peptide sequences, and 26 contained T-cell determinants identified by studies with HLA-transgenic mice and/or reported to be immunogenic in humans. CONCLUSIONS/SIGNIFICANCE: Forty-four (44) pan-DENV sequences of at least 9 amino acids were highly conserved and identical in 80% or more of all recorded DENV sequences, and the majority were found to be immune-relevant by their correspondence to known or putative HLA-restricted T-cell determinants. The conservation of these sequences through the entire recorded DENV genetic history supports their possible value for diagnosis, prophylactic and/or therapeutic applications. The combination of bioinformatics and experimental approaches applied herein provides a framework for large-scale and systematic analysis of conserved and variable sequences of other pathogens, in particular, for rapidly mutating viruses, such as influenza A virus and HIV."],"affiliation":["Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, U.S. Gov't, Non-P.H.S.","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["5 U19 AI56541"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Mice, Transgenic"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]},{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Viral Vaccines"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]},{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["HLA-DR Antigens"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acid Sequence"]}]}],"chemicalList":[{"chemical":[{"name":["Viral Vaccines"],"registryNumber":["0"]},{"name":["Viral Proteins"],"registryNumber":["0"]},{"name":["HLA-DR Antigens"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2491585?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC2491585"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=18698358"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=18698358&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0000272"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["28"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCompletion":["2010-03-10"],"dateOfCreation":["2008-08-13"],"electronicPublicationDate":["2008-08-13"],"firstPublicationDate":["2008-08-13"],"cprojectID":"PMC2491585"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqShhOUS2orL1IOC","_score":1,"_source":{"id":["24421913"],"source":["MED"],"pmid":["24421913"],"pmcid":["PMC3888466"],"doi":["10.1371/journal.pntd.0002636"],"title":["Molecular evolution of Zika virus during its emergence in the 20(th) century."],"authorString":["Faye O, Freire CC, Iamarino A, Faye O, de Oliveira JV, Diallo M, Zanotto PM, Sall AA."],"authorList":[{"author":[{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Institut Pasteur de Dakar, Dakar, Senegal."]},{"fullName":["Freire CC"],"firstName":["Caio C M"],"lastName":["Freire"],"initials":["CC"],"affiliation":["Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Brazil."]},{"fullName":["Iamarino A"],"firstName":["Atila"],"lastName":["Iamarino"],"initials":["A"],"authorId":[{"_":"0000-0003-3904-0248","$":{"type":"ORCID"}}],"affiliation":["Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Brazil."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Institut Pasteur de Dakar, Dakar, Senegal."]},{"fullName":["de Oliveira JV"],"firstName":["Juliana Velasco C"],"lastName":["de Oliveira"],"initials":["JV"],"affiliation":["Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Brazil."]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"],"affiliation":["Institut Pasteur de Dakar, Dakar, Senegal."]},{"fullName":["Zanotto PM"],"firstName":["Paolo M A"],"lastName":["Zanotto"],"initials":["PM"],"affiliation":["Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Brazil."]},{"fullName":["Sall AA"],"firstName":["Amadou Alpha"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Institut Pasteur de Dakar, Dakar, Senegal."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-3904-0248","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["8"],"journalIssueId":["2111164"],"dateOfPublication":["2014 "],"monthOfPublication":["0"],"yearOfPublication":["2014"],"printPublicationDate":["2014-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2014"],"pageInfo":["e2636"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne flavivirus first isolated in Uganda in 1947. Although entomological and virologic surveillance have reported ZIKV enzootic activity in diverse countries of Africa and Asia, few human cases were reported until 2007, when a Zika fever epidemic took place in Micronesia. In the context of West Africa, the WHO Collaborating Centre for Arboviruses and Hemorrhagic Fever at Institut Pasteur of Dakar (http://www.pasteur.fr/recherche/banques/CRORA/) reports the periodic circulation of ZIKV since 1968. Despite several reports on ZIKV, the genetic relationships among viral strains from West Africa remain poorly understood. To evaluate the viral spread and its molecular epidemiology, we investigated 37 ZIKV isolates collected from 1968 to 2002 in six localities in Senegal and Côte d'Ivoire. In addition, we included strains from six other countries. Our results suggested that these two countries in West Africa experienced at least two independent introductions of ZIKV during the 20(th) century, and that apparently these viral lineages were not restricted by mosquito vector species. Moreover, we present evidence that ZIKV has possibly undergone recombination in nature and that a loss of the N154 glycosylation site in the envelope protein was a possible adaptive response to the Aedes dalzieli vector."],"affiliation":["Institut Pasteur de Dakar, Dakar, Senegal."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R01-AI069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["Y"],"descriptorName":["Evolution, Molecular"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Africa, Western"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Genetic Variation"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Epidemiology"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3888466?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3888466"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24421913&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24421913"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0002636"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["21"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," refseq"," pdb"," "]}],"dateOfCompletion":["2014-09-08"],"dateOfCreation":["2014-01-14"],"dateOfRevision":["2016-02-04"],"electronicPublicationDate":["2014-01-09"],"firstPublicationDate":["2014-01-09"],"cprojectID":"PMC3888466"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSXJ9hGurOzZIXq","_score":1,"_source":{"id":["23986750"],"source":["MED"],"pmid":["23986750"],"pmcid":["PMC3749491"],"doi":["10.3389/fmicb.2013.00234"],"title":["The MHC-II transactivator CIITA, a restriction factor against oncogenic HTLV-1 and HTLV-2 retroviruses: similarities and differences in the inhibition of Tax-1 and Tax-2 viral transactivators."],"authorString":["Forlani G, Abdallah R, Accolla RS, Tosi G."],"authorList":[{"author":[{"fullName":["Forlani G"],"firstName":["Greta"],"lastName":["Forlani"],"initials":["G"],"authorId":[{"_":"0000-0003-1987-7761","$":{"type":"ORCID"}}],"affiliation":["Laboratory of General Pathology and Immunology, Department of Surgical and Morphological Sciences, University of Insubria Varese, Italy."]},{"fullName":["Abdallah R"],"firstName":["Rawan"],"lastName":["Abdallah"],"initials":["R"]},{"fullName":["Accolla RS"],"firstName":["Roberto S"],"lastName":["Accolla"],"initials":["RS"],"authorId":[{"_":"0000-0003-2493-7378","$":{"type":"ORCID"}}]},{"fullName":["Tosi G"],"firstName":["Giovanna"],"lastName":["Tosi"],"initials":["G"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-1987-7761","$":{"type":"ORCID"}},{"_":"0000-0003-2493-7378","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["4"],"journalIssueId":["2003507"],"dateOfPublication":["2013 "],"monthOfPublication":["0"],"yearOfPublication":["2013"],"printPublicationDate":["2013-01-01"],"journal":[{"title":["Frontiers in microbiology"],"ISOAbbreviation":["Front Microbiol"],"medlineAbbreviation":["Front Microbiol"],"NLMid":["101548977"],"ESSN":["1664-302X"]}]}],"pubYear":["2013"],"pageInfo":["234"],"abstractText":["The activation of CD4(+) T helper cells is strictly dependent on the presentation of antigenic peptides by MHC class II (MHC-II) molecules. MHC-II expression is primarily regulated at the transcriptional level by the AIR-1 gene product CIITA (class II transactivator). Thus, CIITA plays a pivotal role in the triggering of the adaptive immune response against pathogens. Besides this well known function, we recently found that CIITA acts as an endogenous restriction factor against HTLV-1 (human T cell lymphotropic virus type 1) and HTLV-2 oncogenic retroviruses by targeting their viral transactivators Tax-1 and Tax-2, respectively. Here we review our findings on CIITA-mediated inhibition of viral replication and discuss similarities and differences in the molecular mechanisms by which CIITA specifically counteracts the function of Tax-1 and Tax-2 molecules. The dual function of CIITA as a key regulator of adaptive and intrinsic immunity represents a rather unique example of adaptation of host-derived factors against pathogen infections during evolution. "],"affiliation":["Laboratory of General Pathology and Immunology, Department of Surgical and Morphological Sciences, University of Insubria Varese, Italy."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["viral replication","CIITA","Restriction Factors","Htlv-1 Tax-1","Htlv-2 Tax-2"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3749491?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3749491"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=23986750&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=23986750"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fmicb.2013.00234"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2013-08-29"],"dateOfCreation":["2013-08-29"],"dateOfRevision":["2014-01-24"],"electronicPublicationDate":["2013-08-22"],"firstPublicationDate":["2013-08-22"],"cprojectID":"PMC3749491"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSc_8VqgcuJwvf1","_score":1,"_source":{"id":["24478593"],"source":["MED"],"pmid":["24478593"],"pmcid":["PMC3880869"],"doi":["10.2149/tmh.2013-08"],"title":["Distribution of mosquito larvae on kosrae island, kosrae state, the federated States of micronesia."],"authorString":["Noda S, Yamamoto S, Toma T, Taulung L."],"authorList":[{"author":[{"fullName":["Noda S"],"firstName":["Shinichi"],"lastName":["Noda"],"initials":["S"],"affiliation":["Kagoshima University Research Center for the Pacific Islands, Kagoshima, Japan."]},{"fullName":["Yamamoto S"],"firstName":["Sota"],"lastName":["Yamamoto"],"initials":["S"],"affiliation":["Kagoshima University Research Center for the Pacific Islands, Kagoshima, Japan."]},{"fullName":["Toma T"],"firstName":["Takako"],"lastName":["Toma"],"initials":["T"],"affiliation":["School of Health, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan."]},{"fullName":["Taulung L"],"firstName":["Livinson"],"lastName":["Taulung"],"initials":["L"],"affiliation":["Department of Health Services, Kosrae State Government, Kosrae, Federated States of Micronesia."]}]}],"journalInfo":[{"issue":["4"],"volume":["41"],"journalIssueId":["2120553"],"dateOfPublication":["2013 Dec"],"monthOfPublication":["12"],"yearOfPublication":["2013"],"printPublicationDate":["2013-12-01"],"journal":[{"title":["Tropical medicine and health"],"ISOAbbreviation":["Trop Med Health"],"medlineAbbreviation":["Trop Med Health"],"NLMid":["101215093"],"ISSN":["1348-8945"],"ESSN":["1349-4147"]}]}],"pubYear":["2013"],"pageInfo":["157-161"],"abstractText":["Surveys of mosquito larvae were carried out in six areas of Kosrae Island, Kosrae State, the Federated States of Micronesia in December 2009 and June 2012. A total of 962 larvae of six species were collected from 106 natural and artificial habitats. They were identified as Aedes aegypti, Ae. albopictus, Ae. marshallensis, Culex quinquefasciatus, Cx. annulirostris, and Cx. kusaiensis. This is the first report from Kosrae Island for three of these species-Ae. marshallensis, Cx. quinquefasciatus, and Cx. annulirostris. The most abundant species was Ae. albopictus, followed by Ae. marshallensis, and these two species were found in all areas. Relatively large numbers of Cx. quinquefasciatus and Cx. kusaiensis were found in five areas. Fewer Cx. annulirostris were found, and only in three areas. Aedes aegypti larvae were collected from a single habitat at Tafunsak in 2009. To prevent the outbreak of dengue fever, environmental management should focus on the destruction, alteration, disposal and recycling of containers that produce larger numbers of adult Aedes mosquitoes. "],"affiliation":["Kagoshima University Research Center for the Pacific Islands, Kagoshima, Japan."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes aegypti","Aedes albopictus","Aedes Marshallensis","Federated States Of Micronesia","Kosrae State","Mosquito Fauna"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3880869?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3880869"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24478593"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24478593&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2149/tmh.2013-08"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2014-01-30"],"dateOfCreation":["2014-01-30"],"dateOfRevision":["2014-02-03"],"electronicPublicationDate":["2013-11-28"],"firstPublicationDate":["2013-11-28"],"cprojectID":"PMC3880869"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrrzhOUS2orL1IRh","_score":1,"_source":{"id":["27423687"],"source":["MED"],"pmid":["27423687"],"pmcid":["PMC4947515"],"doi":["10.1186/s12978-016-0200-6"],"title":["Zika virus infection in pregnant women in Honduras: study protocol."],"authorString":["Buekens P, Alger J, Althabe F, Bergel E, Berrueta AM, Bustillo C, Cafferata ML, Harville E, Rosales K, Wesson DM, Zuniga C, ZIPH Working Group."],"authorList":[{"author":[{"fullName":["Buekens P"],"firstName":["Pierre"],"lastName":["Buekens"],"initials":["P"],"authorId":[{"_":"0000-0002-4294-8559","$":{"type":"ORCID"}}],"affiliation":["School of Public Health and Tropical Medicine, Tulane University, 1440 Canal Street, Ste. 2430, New Orleans, LA, 70112, USA. pbuekens@tulane.edu."]},{"fullName":["Alger J"],"firstName":["Jackeline"],"lastName":["Alger"],"initials":["J"],"affiliation":["Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Hospital Escuela Universitario, Facultad de Ciencias Médicas, UNAH, Calle La Salud, Boulevard a Suyapa, Colonia Quezada, Tegucigalpa, Honduras."]},{"fullName":["Althabe F"],"firstName":["Fernando"],"lastName":["Althabe"],"initials":["F"],"affiliation":["Instituto de Efectividad Clínica y Sanitaria (IECS), Dr. Emilio Ravignani 2024, C1414CPV, Buenos Aires, Argentina."]},{"fullName":["Bergel E"],"firstName":["Eduardo"],"lastName":["Bergel"],"initials":["E"],"affiliation":["Instituto de Efectividad Clínica y Sanitaria (IECS), Dr. Emilio Ravignani 2024, C1414CPV, Buenos Aires, Argentina."]},{"fullName":["Berrueta AM"],"firstName":["Amanda M"],"lastName":["Berrueta"],"initials":["AM"],"affiliation":["Instituto de Efectividad Clínica y Sanitaria (IECS), Dr. Emilio Ravignani 2024, C1414CPV, Buenos Aires, Argentina."]},{"fullName":["Bustillo C"],"firstName":["Carolina"],"lastName":["Bustillo"],"initials":["C"],"affiliation":["Departamento de Ginecología y Obstetricia, Hospital Escuela, Calle La Salud, Boulevard a Suyapa, Colonia Quezada, Tegucigalpa, Honduras."]},{"fullName":["Cafferata ML"],"firstName":["Maria-Luisa"],"lastName":["Cafferata"],"initials":["ML"],"affiliation":["Unidad de Investigación Clínica y Epidemiológica Montevideo (UNICEM), Hospital de Clinicas, Avenida Italia s/n - piso 4°, Montevideo, 11600, Uruguay."]},{"fullName":["Harville E"],"firstName":["Emily"],"lastName":["Harville"],"initials":["E"],"affiliation":["Department of Epidemiology, Tulane School of Public Health and Tropical Medicine, Tulane University, Ste. 2012, 1440 Canal St., New Orleans, LA, 70112, USA."]},{"fullName":["Rosales K"],"firstName":["Karla"],"lastName":["Rosales"],"initials":["K"],"affiliation":["Región Sanitaria Metropolitana Distrito Central de Francisco Morazán, Tegucigalpa, Honduras."]},{"fullName":["Wesson DM"],"firstName":["Dawn M"],"lastName":["Wesson"],"initials":["DM"],"affiliation":["School of Public Health and Tropical Medicine, Tulane University, 1324 Tulane Avenue, JBJ Building, Room 509, New Orleans, LA, 70112, USA."]},{"fullName":["Zuniga C"],"firstName":["Concepcion"],"lastName":["Zuniga"],"initials":["C"],"affiliation":["Instituto de Enfermedades Infecciosas y Parasitología Antonio Vidal; Hospital Escuela Universitario, Calle La Salud, Boulevard a Suyapa, Colonia Quezada, Tegucigalpa, Honduras."]},{"collectiveName":["ZIPH Working Group"]}]}],"investigatorList":[{"investigator":[{"fullName":["Buekens P"],"firstName":["Pierre"],"lastName":["Buekens"],"initials":["P"]},{"fullName":["Alger J"],"firstName":["Jackeline"],"lastName":["Alger"],"initials":["J"]},{"fullName":["Althabe F"],"firstName":["Fernando"],"lastName":["Althabe"],"initials":["F"]},{"fullName":["Bergel E"],"firstName":["Eduardo"],"lastName":["Bergel"],"initials":["E"]},{"fullName":["Berrueta A"],"firstName":["AmandaM"],"lastName":["Berrueta"],"initials":["A"]},{"fullName":["Bustillo C"],"firstName":["Carolina"],"lastName":["Bustillo"],"initials":["C"]},{"fullName":["Cafferata M"],"firstName":["MariaLuisa"],"lastName":["Cafferata"],"initials":["M"]},{"fullName":["Harville E"],"firstName":["Emily"],"lastName":["Harville"],"initials":["E"]},{"fullName":["Largaespada J"],"firstName":["Jorge"],"lastName":["Largaespada"],"initials":["J"]},{"fullName":["de Rivera I"],"firstName":["IvetteLorenzana"],"lastName":["de Rivera"],"initials":["I"]},{"fullName":["Matta C"],"firstName":["Carlos"],"lastName":["Matta"],"initials":["C"]},{"fullName":["Pastrana K"],"firstName":["Karla"],"lastName":["Pastrana"],"initials":["K"]},{"fullName":["Rosales K"],"firstName":["Karla"],"lastName":["Rosales"],"initials":["K"]},{"fullName":["Wesson D"],"firstName":["Dawn"],"lastName":["Wesson"],"initials":["D"]},{"fullName":["Zúniga C"],"firstName":["Concepción"],"lastName":["Zúniga"],"initials":["C"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-4294-8559","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["13"],"journalIssueId":["2358909"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Reproductive health"],"ISOAbbreviation":["Reprod Health"],"medlineAbbreviation":["Reprod Health"],"NLMid":["101224380"],"ESSN":["1742-4755"]}]}],"pubYear":["2016"],"pageInfo":["82"],"abstractText":["Although there is increasing evidence for a relationship between symptomatic Zika virus (ZIKV) maternal infection, and microcephaly, a firm causal relation has yet to be established by epidemiologic studies. Studies also need to be conducted in recently infected settings. Our objectives are to assess the frequency of ZIKV infection during pregnancy in Honduras and the association of microcephaly with ZIKV infection.We will perform a prospective study enrolling pregnant women at their first antenatal visit and following them up until delivery. At the time of enrollment, women will be interviewed to collect socio-demographic data, data needed to locate them for potential additional follow-up, and data about ZIKV symptoms during pregnancy. We will also collect maternal blood as soon as possible after enrollment. A probable maternal ZIKV infection will be defined as positive for maternal ZIKV IgM. A confirmed maternal ZIKV infection will be defined as positive for ZIKV IgM confirmed by plaque reduction neutralization test. Microcephaly at birth will be defined as an occipito-frontal circumference <2SD for sex and gestational age. Our objective is to enroll 2000 pregnant women. In a first step, we will follow a case cohort design and only analyze blood samples for cases and a sub-cohort of 200 women randomly selected. Blood samples for the entire population will be analyzed at a later stage if funds are available.This protocol was designed to be implemented with minimal resources. It allows a cohort to be built, which could be a foundation for future in-depth and follow-up studies."],"affiliation":["School of Public Health and Tropical Medicine, Tulane University, 1440 Canal Street, Ste. 2430, New Orleans, LA, 70112, USA. pbuekens@tulane.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Pregnancy","Microcephaly","Honduras","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947515?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947515"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12978-016-0200-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-18"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-07-16"],"firstPublicationDate":["2016-07-16"],"cprojectID":"PMC4947515"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrsr_8VqgcuJwvjX","_score":1,"_source":{"id":["27275816"],"source":["MED"],"pmid":["27275816"],"pmcid":["PMC4926380"],"doi":["10.3390/ijms17060846"],"title":["Human Saliva Collection Devices for Proteomics: An Update."],"authorString":["Khurshid Z, Zohaib S, Najeeb S, Zafar MS, Slowey PD, Almas K."],"authorList":[{"author":[{"fullName":["Khurshid Z"],"firstName":["Zohaib"],"lastName":["Khurshid"],"initials":["Z"],"affiliation":["College of Dentistry, King Faisal University, Al-Hofuf, Al-Ahsa 31982, Saudi Arabia. drzohaibkhurshid@gmail.com."]},{"fullName":["Zohaib S"],"firstName":["Sana"],"lastName":["Zohaib"],"initials":["S"],"affiliation":["Department of Biomedical Engineering, School of Engineering, King Faisal University, Al-Hofuf, Al-Ahsa 31982, Saudi Arabia. szohaib@kfu.edu.sa."]},{"fullName":["Najeeb S"],"firstName":["Shariq"],"lastName":["Najeeb"],"initials":["S"],"authorId":[{"_":"0000-0002-3803-6055","$":{"type":"ORCID"}}],"affiliation":["Restorative Dental Sciences, Al-Farabi Colleges, Riyadh 361724, Saudi Arabia. shariqnajeeb@gmail.com."]},{"fullName":["Zafar MS"],"firstName":["Muhammad Sohail"],"lastName":["Zafar"],"initials":["MS"],"affiliation":["Department of Restorative Dentistry, College of Dentistry, Taibah University, Al Madinah, Al Munawwarah 41311, Saudi Arabia. MZAFAR@taibahu.edu.sa."]},{"fullName":["Slowey PD"],"firstName":["Paul D"],"lastName":["Slowey"],"initials":["PD"],"affiliation":["Oasis Diagnostics® Corporation, 15720 NE 31st Avenue, Vancouver, WA 98686, USA. pds@4saliva.com."]},{"fullName":["Almas K"],"firstName":["Khalid"],"lastName":["Almas"],"initials":["K"],"affiliation":["Division of Periodontology, College of Dentistry, University of Dammam, Dammam 31441, Saudi Arabia. Kalmas@uod.edu.sa."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3803-6055","$":{"type":"ORCID"}},{"_":"0000-0001-7998-7335","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["17"],"journalIssueId":["2412418"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["International journal of molecular sciences"],"ISOAbbreviation":["Int J Mol Sci"],"medlineAbbreviation":["Int J Mol Sci"],"NLMid":["101092791"],"ESSN":["1422-0067"]}]}],"pubYear":["2016"],"abstractText":["There has been a rapid growth in the interest and adaptation of saliva as a diagnostic specimen over the last decade, and in the last few years in particular, there have been major developments involving the application of saliva as a clinically relevant specimen. Saliva provides a \"window\" into the oral and systemic health of an individual, and like other bodily fluids, saliva can be analyzed and studied to diagnose diseases. With the advent of new, more sensitive technologies to detect smaller concentrations of analytes in saliva relative to blood levels, there have been a number of critical developments in the field that we will describe. In particular, recent advances in standardized saliva collection devices that were not available three to four years ago, have made it easy for safe, simple, and non-invasive collection of samples to be carried out from patients. With the availability of these new technologies, we believe that in the next decade salivary proteomics will make it possible to predict and diagnose oral as well as systemic diseases, cancer, and infectious diseases, among others. The aim of this article is to review recent developments and advances in the area of saliva specimen collection devices and applications that will advance the field of proteomics."],"affiliation":["College of Dentistry, King Faisal University, Al-Hofuf, Al-Ahsa 31982, Saudi Arabia. drzohaibkhurshid@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Proteomics","Omics","Salivary Biomarkers","Salivaomics","Saliva Collection Devices"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926380?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4926380"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijms17060846"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-06"],"firstPublicationDate":["2016-06-06"],"cprojectID":"PMC4926380"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtDJ9hGurOzZIbY","_score":1,"_source":{"id":["27508088"],"source":["MED"],"pmid":["27508088"],"pmcid":["PMC4957610"],"doi":["10.5365/wpsar.2016.7.1.002"],"title":["External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015."],"authorString":["Soh LT, Squires RC, Tan LK, Pok KY, Yang H, Liew C, Shah AS, Aaskov J, Abubakar S, Hasabe F, Ng LC, Konings F."],"authorList":[{"author":[{"fullName":["Soh LT"],"firstName":["Li Ting"],"lastName":["Soh"],"initials":["LT"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore.; Both authors contributed equally in the writing of this paper."]},{"fullName":["Squires RC"],"firstName":["Raynal C"],"lastName":["Squires"],"initials":["RC"],"affiliation":["Emerging Disease Surveillance and Response, Division of Health Security and Emergencies, World Health Organization Regional Office for the Western Pacific, Manila, Philippines.; Both authors contributed equally in the writing of this paper."]},{"fullName":["Tan LK"],"firstName":["Li Kiang"],"lastName":["Tan"],"initials":["LK"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Pok KY"],"firstName":["Kwoon Yong"],"lastName":["Pok"],"initials":["KY"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Yang H"],"firstName":["HuiTing"],"lastName":["Yang"],"initials":["H"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Liew C"],"firstName":["Christina"],"lastName":["Liew"],"initials":["C"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Shah AS"],"firstName":["Aparna Singh"],"lastName":["Shah"],"initials":["AS"],"affiliation":["Blood Safety and Laboratory Technology, Communicable Diseases Department, World Health Organization Regional Office for the South East-Asia, New Delhi, India ."]},{"fullName":["Aaskov J"],"firstName":["John"],"lastName":["Aaskov"],"initials":["J"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Abubakar S"],"firstName":["Sazaly"],"lastName":["Abubakar"],"initials":["S"],"affiliation":["WHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia ."]},{"fullName":["Hasabe F"],"firstName":["Futoshi"],"lastName":["Hasabe"],"initials":["F"],"affiliation":["WHO Collaborating Centre for Reference and Research on Tropical and Emerging Virus Diseases, Nagasaki University, Nagasaki, Japan ."]},{"fullName":["Ng LC"],"firstName":["Lee Ching"],"lastName":["Ng"],"initials":["LC"],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore ."]},{"fullName":["Konings F"],"firstName":["Frank"],"lastName":["Konings"],"initials":["F"],"affiliation":["Emerging Disease Surveillance and Response, Division of Health Security and Emergencies, World Health Organization Regional Office for the Western Pacific, Manila, Philippines ."]}]}],"journalInfo":[{"issue":["2"],"volume":["7"],"journalIssueId":["2440062"],"dateOfPublication":["2016 Apr-Jun"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Western Pacific surveillance and response journal : WPSAR"],"ISOAbbreviation":["Western Pac Surveill Response J"],"medlineAbbreviation":["Western Pac Surveill Response J"],"NLMid":["101558993"],"ISSN":["2094-7321"],"ESSN":["2094-7313"]}]}],"pubYear":["2016"],"pageInfo":["26-34"],"abstractText":["To conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013.Twenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. Module B contained human serum samples for the detection of anti-DENV antibodies.Among 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT-PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT-PCR. Ten of 15 (66.7%) laboratories performing NS1 antigen assays obtained the correct results. In Module B, 18/23 (78.3%) and 20/20 (100%) of laboratories correctly detected anti-DENV IgM and IgG, respectively. Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories.Accurate laboratory testing is a critical component of dengue and chikungunya surveillance and control. This second round of EQA reveals good proficiency in molecular and serological diagnostics of these diseases in the Asia Pacific region. Further comprehensive diagnostic testing, including testing for Zika virus, should comprise future iterations of the EQA."],"affiliation":["WHO Collaborating Centre for Reference and Research of Arbovirus and their Associated Vectors, Environmental Health Institute, National Environment Agency, Singapore.; Both authors contributed equally in the writing of this paper."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957610?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957610"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.5365/WPSAR.2016.7.1.002"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-10"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-04-22"],"firstPublicationDate":["2016-04-22"],"cprojectID":"PMC4957610"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrtb_8VqgcuJwvjf","_score":1,"_source":{"id":["27429485"],"source":["MED"],"pmid":["27429485"],"pmcid":["PMC4933145"],"doi":["10.2471/blt.16.010716"],"title":["Public health round-up."],"journalInfo":[{"issue":["7"],"volume":["94"],"journalIssueId":["2430669"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Bulletin of the World Health Organization"],"ISOAbbreviation":["Bull. World Health Organ."],"medlineAbbreviation":["Bull World Health Organ"],"NLMid":["7507052"],"ISSN":["0042-9686"],"ESSN":["1564-0604"]}]}],"pubYear":["2016"],"pageInfo":["485-486"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933145?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4933145"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2471/BLT.16.010716"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4933145"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrt8hOUS2orL1IRp","_score":1,"_source":{"id":["27457156"],"source":["MED"],"pmid":["27457156"],"pmcid":["PMC4960544"],"doi":["10.1038/srep30357"],"title":["Production of full-length soluble Plasmodium falciparum RH5 protein vaccine using a Drosophila melanogaster Schneider 2 stable cell line system."],"authorString":["Hjerrild KA, Jin J, Wright KE, Brown RE, Marshall JM, Labbé GM, Silk SE, Cherry CJ, Clemmensen SB, Jørgensen T, Illingworth JJ, Alanine DG, Milne KH, Ashfield R, de Jongh WA, Douglas AD, Higgins MK, Draper SJ."],"authorList":[{"author":[{"fullName":["Hjerrild KA"],"firstName":["Kathryn A"],"lastName":["Hjerrild"],"initials":["KA"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Jin J"],"firstName":["Jing"],"lastName":["Jin"],"initials":["J"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Wright KE"],"firstName":["Katherine E"],"lastName":["Wright"],"initials":["KE"],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK."]},{"fullName":["Brown RE"],"firstName":["Rebecca E"],"lastName":["Brown"],"initials":["RE"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Marshall JM"],"firstName":["Jennifer M"],"lastName":["Marshall"],"initials":["JM"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Labbé GM"],"firstName":["Geneviève M"],"lastName":["Labbé"],"initials":["GM"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Silk SE"],"firstName":["Sarah E"],"lastName":["Silk"],"initials":["SE"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Cherry CJ"],"firstName":["Catherine J"],"lastName":["Cherry"],"initials":["CJ"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Clemmensen SB"],"firstName":["Stine B"],"lastName":["Clemmensen"],"initials":["SB"],"affiliation":["ExpreS2ion Biotechnologies, SCION-DTU Science Park, Agern Allé 1, Hørsholm DK-2970, Denmark."]},{"fullName":["Jørgensen T"],"firstName":["Thomas"],"lastName":["Jørgensen"],"initials":["T"],"affiliation":["ExpreS2ion Biotechnologies, SCION-DTU Science Park, Agern Allé 1, Hørsholm DK-2970, Denmark."]},{"fullName":["Illingworth JJ"],"firstName":["Joseph J"],"lastName":["Illingworth"],"initials":["JJ"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Alanine DG"],"firstName":["Daniel G W"],"lastName":["Alanine"],"initials":["DG"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Milne KH"],"firstName":["Kathryn H"],"lastName":["Milne"],"initials":["KH"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Ashfield R"],"firstName":["Rebecca"],"lastName":["Ashfield"],"initials":["R"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["de Jongh WA"],"firstName":["Willem A"],"lastName":["de Jongh"],"initials":["WA"],"affiliation":["ExpreS2ion Biotechnologies, SCION-DTU Science Park, Agern Allé 1, Hørsholm DK-2970, Denmark."]},{"fullName":["Douglas AD"],"firstName":["Alexander D"],"lastName":["Douglas"],"initials":["AD"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]},{"fullName":["Higgins MK"],"firstName":["Matthew K"],"lastName":["Higgins"],"initials":["MK"],"affiliation":["Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK."]},{"fullName":["Draper SJ"],"firstName":["Simon J"],"lastName":["Draper"],"initials":["SJ"],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["30357"],"abstractText":["The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) has recently emerged as a leading candidate antigen against the blood-stage human malaria parasite. However it has proved challenging to identify a heterologous expression platform that can produce a soluble protein-based vaccine in a manner compliant with current Good Manufacturing Practice (cGMP). Here we report the production of full-length PfRH5 protein using a cGMP-compliant platform called ExpreS(2), based on a Drosophila melanogaster Schneider 2 (S2) stable cell line system. Five sequence variants of PfRH5 were expressed that differed in terms of mutagenesis strategies to remove potential N-linked glycans. All variants bound the PfRH5 receptor basigin and were recognized by a panel of monoclonal antibodies. Analysis following immunization of rabbits identified quantitative and qualitative differences in terms of the functional IgG antibody response against the P. falciparum parasite. The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen."],"affiliation":["The Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, OX3 7DQ, UK."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960544?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4960544"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep30357"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCreation":["2016-07-26"],"dateOfRevision":["2016-08-07"],"electronicPublicationDate":["2016-07-26"],"firstPublicationDate":["2016-07-26"],"cprojectID":"PMC4960544"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwLhOUS2orL1IRz","_score":1,"_source":{"id":["27479197"],"source":["MED"],"pmid":["27479197"],"pmcid":["PMC4968803"],"doi":["10.1371/journal.pntd.0004871"],"title":["Limited Effects of Type I Interferons on Kyasanur Forest Disease Virus in Cell Culture."],"authorString":["Cook BW, Ranadheera C, Nikiforuk AM, Cutts TA, Kobasa D, Court DA, Theriault SS."],"authorList":[{"author":[{"fullName":["Cook BW"],"firstName":["Bradley W M"],"lastName":["Cook"],"initials":["BW"],"affiliation":["Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."]},{"fullName":["Ranadheera C"],"firstName":["Charlene"],"lastName":["Ranadheera"],"initials":["C"],"affiliation":["High Containment Respiratory Viruses Group, Special Pathogens Program, National Microbiology Laboratory at the Canadian Science Centre for Human and Animal Health, Public Health Agency of Canada, Winnipeg, Manitoba, Canada."]},{"fullName":["Nikiforuk AM"],"firstName":["Aidan M"],"lastName":["Nikiforuk"],"initials":["AM"],"affiliation":["Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."]},{"fullName":["Cutts TA"],"firstName":["Todd A"],"lastName":["Cutts"],"initials":["TA"],"affiliation":["Applied Biosafety Research Program, National Microbiology Laboratory at the Canadian Science Centre for Human and Animal Health and National Microbiology Laboratory at the J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada."]},{"fullName":["Kobasa D"],"firstName":["Darwyn"],"lastName":["Kobasa"],"initials":["D"],"affiliation":["Department of Medical Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."]},{"fullName":["Court DA"],"firstName":["Deborah A"],"lastName":["Court"],"initials":["DA"],"affiliation":["Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."]},{"fullName":["Theriault SS"],"firstName":["Steven S"],"lastName":["Theriault"],"initials":["SS"],"affiliation":["Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004871"],"abstractText":["The tick-borne flavivirus, Kyasanur Forest disease virus (KFDV) causes seasonal infections and periodic outbreaks in south-west India. The current vaccine offers poor protection with reported issues of coverage and immunogenicity. Since there are no approved prophylactic therapeutics for KFDV, type I IFN-α/β subtypes were assessed for antiviral potency against KFDV in cell culture.The continued passage of KFDV-infected cells with re-administered IFN-α2a treatment did not eliminate KFDV and had little effect on infectious particle production whereas the IFN-sensitive, green fluorescent protein-expressing vesicular stomatitis virus (VSV-GFP) infection was controlled. Further evaluation of the other IFN-α/β subtypes versus KFDV infection indicated that single treatments of either IFN-αWA and IFN-αΚ appeared to be more effective than IFN-α2a at reducing KFDV titres. Concentration-dependent analysis of these IFN-α/β subtypes revealed that regardless of subtype, low concentrations of IFN were able to limit cytopathic effects (CPE), while significantly higher concentrations were needed for inhibition of virion release. Furthermore, expression of the KFDV NS5 in cell culture before IFN addition enabled VSV-GFP to overcome the effects of IFN-α/β signalling, producing a robust infection.Treatment of cell culture with IFN does not appear to be suitable for KFDV eradication and the assay used for such studies should be carefully considered. Further, it appears that the NS5 protein is sufficient to permit KFDV to bypass the antiviral properties of IFN. We suggest that other prophylactic therapeutics should be evaluated in place of IFN for treatment of individuals with KFDV disease."],"affiliation":["Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4968803?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4968803"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004871"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-19"],"electronicPublicationDate":["2016-08-01"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4968803"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrxY_8VqgcuJwvjn","_score":1,"_source":{"id":["27353759"],"source":["MED"],"pmid":["27353759"],"pmcid":["PMC4937216"],"doi":["10.1128/mbio.00804-16"],"title":["Dengue Virus Genome Uncoating Requires Ubiquitination."],"authorString":["Byk LA, Iglesias NG, De Maio FA, Gebhard LG, Rossi M, Gamarnik AV."],"authorList":[{"author":[{"fullName":["Byk LA"],"firstName":["Laura A"],"lastName":["Byk"],"initials":["LA"],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina."]},{"fullName":["Iglesias NG"],"firstName":["Néstor G"],"lastName":["Iglesias"],"initials":["NG"],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina."]},{"fullName":["De Maio FA"],"firstName":["Federico A"],"lastName":["De Maio"],"initials":["FA"],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina."]},{"fullName":["Gebhard LG"],"firstName":["Leopoldo G"],"lastName":["Gebhard"],"initials":["LG"],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina."]},{"fullName":["Rossi M"],"firstName":["Mario"],"lastName":["Rossi"],"initials":["M"],"affiliation":["Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA)-CONICET-Partner Institute of the Max Planck Society, Polo Científico Tecnológico, Buenos Aires, Argentina."]},{"fullName":["Gamarnik AV"],"firstName":["Andrea V"],"lastName":["Gamarnik"],"initials":["AV"],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina agamarnik@leloir.org.ar."]}]}],"journalInfo":[{"issue":["3"],"volume":["7"],"journalIssueId":["2404410"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"abstractText":["The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile viruses. Currently, dengue virus is one of the most significant human viral pathogens transmitted by mosquitoes and is responsible for about 390 million infections every year around the world. Here, we examined for the first time molecular aspects of dengue virus genome uncoating. We followed the fate of the capsid protein and RNA genome early during infection and found that capsid is degraded after viral internalization by the host ubiquitin-proteasome system. However, proteasome activity and capsid degradation were not necessary to free the genome for initial viral translation. Unexpectedly, genome uncoating was blocked by inhibiting ubiquitination. Using different assays to bypass entry and evaluate the first rounds of viral translation, a narrow window of time during infection that requires ubiquitination but not proteasome activity was identified. In this regard, ubiquitin E1-activating enzyme inhibition was sufficient to stabilize the incoming viral genome in the cytoplasm of infected cells, causing its retention in either endosomes or nucleocapsids. Our data support a model in which dengue virus genome uncoating requires a nondegradative ubiquitination step, providing new insights into this crucial but understudied viral process.Dengue is the most significant arthropod-borne viral infection in humans. Although the number of cases increases every year, there are no approved therapeutics available for the treatment of dengue infection, and many basic aspects of the viral biology remain elusive. After entry, the viral membrane must fuse with the endosomal membrane to deliver the viral genome into the cytoplasm for translation and replication. A great deal of information has been obtained in the last decade regarding molecular aspects of the fusion step, but little is known about the events that follow this process, which leads to viral RNA release from the nucleocapsid. Here, we investigated the fate of nucleocapsid components (capsid protein and viral genome) during the infection process and found that capsid is degraded by the ubiquitin-proteasome system. However, in contrast to that observed for other RNA and DNA viruses, dengue virus capsid degradation was not responsible for genome uncoating. Interestingly, we found that dengue virus genome release requires a nondegradative ubiquitination step. These results provide the first insights into dengue virus uncoating and present new opportunities for antiviral intervention."],"affiliation":["Fundación Instituto Leloir, CONICET, Buenos Aires, Argentina."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4937216?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4937216"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.00804-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-29"],"dateOfRevision":["2016-07-13"],"electronicPublicationDate":["2016-06-28"],"firstPublicationDate":["2016-06-28"],"cprojectID":"PMC4937216"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnryeJ9hGurOzZIbs","_score":1,"_source":{"id":["27485513"],"source":["MED"],"pmid":["27485513"],"pmcid":["PMC4971666"],"doi":["10.1186/s40249-016-0162-5"],"title":["Prevalence of pathogenic free-living amoeba and other protozoa in natural and communal piped tap water from Queen Elizabeth protected area, Uganda."],"authorString":["Sente C, Erume J, Naigaga I, Mulindwa J, Ochwo S, Magambo PK, Namara BG, Kato CD, Sebyatika G, Muwonge K, Ocaido M."],"authorList":[{"author":[{"fullName":["Sente C"],"firstName":["Celsus"],"lastName":["Sente"],"initials":["C"],"affiliation":["Department of Wildlife and Aquatic Animal Resources (WAAR), School of Veterinary Medicine and Animal Resources (SVAR), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda. csente37@gmail.com."]},{"fullName":["Erume J"],"firstName":["Joseph"],"lastName":["Erume"],"initials":["J"],"affiliation":["Department of Biomolecular Resources and Biolab Sciences, School of Bio-security, Biotechnical and Laboratory Sciences (SBLS), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]},{"fullName":["Naigaga I"],"firstName":["Irene"],"lastName":["Naigaga"],"initials":["I"],"affiliation":["Department of Wildlife and Aquatic Animal Resources (WAAR), School of Veterinary Medicine and Animal Resources (SVAR), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]},{"fullName":["Mulindwa J"],"firstName":["Julius"],"lastName":["Mulindwa"],"initials":["J"],"affiliation":["Department of Biochemistry and Sports Science (BSS), College of Natural Sciences (CONAS), Makerere University, P.O. Box 7062, Kampala, Uganda."]},{"fullName":["Ochwo S"],"firstName":["Sylvester"],"lastName":["Ochwo"],"initials":["S"],"affiliation":["Department of Biomolecular Resources and Biolab Sciences, School of Bio-security, Biotechnical and Laboratory Sciences (SBLS), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]},{"fullName":["Magambo PK"],"firstName":["Phillip Kimuda"],"lastName":["Magambo"],"initials":["PK"],"affiliation":["Department of Biomolecular Resources and Biolab Sciences, School of Bio-security, Biotechnical and Laboratory Sciences (SBLS), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]},{"fullName":["Namara BG"],"firstName":["Benigna Gabriela"],"lastName":["Namara"],"initials":["BG"],"affiliation":["Medical Research Council (MRC)/Uganda Virus Research Institute (UVRI), Research Unit on AIDS, P.O.Box 49, Entebbe, Uganda."]},{"fullName":["Kato CD"],"firstName":["Charles Drago"],"lastName":["Kato"],"initials":["CD"],"affiliation":["Department of Biomolecular Resources and Biolab Sciences, School of Bio-security, Biotechnical and Laboratory Sciences (SBLS), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]},{"fullName":["Sebyatika G"],"firstName":["George"],"lastName":["Sebyatika"],"initials":["G"],"affiliation":["Department of Molecular Biology, Vrije Universiteit, Pleinlaan 21050, Brussels, Belgium."]},{"fullName":["Muwonge K"],"firstName":["Kevin"],"lastName":["Muwonge"],"initials":["K"],"affiliation":["Department of Molecular Biology, Vrije Universiteit, Pleinlaan 21050, Brussels, Belgium."]},{"fullName":["Ocaido M"],"firstName":["Michael"],"lastName":["Ocaido"],"initials":["M"],"affiliation":["Department of Wildlife and Aquatic Animal Resources (WAAR), School of Veterinary Medicine and Animal Resources (SVAR), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2376921"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infectious diseases of poverty"],"ISOAbbreviation":["Infect Dis Poverty"],"medlineAbbreviation":["Infect Dis Poverty"],"NLMid":["101606645"],"ESSN":["2049-9957"]}]}],"pubYear":["2016"],"pageInfo":["68"],"abstractText":["Pathogenic water dwelling protozoa such as Acanthamoeba spp., Hartmannella spp., Naegleria spp., Cryptosporidium spp. and Giardia spp. are often responsible for devastating illnesses especially in children and immunocompromised individuals, yet their presence and prevalence in certain environment in sub-Saharan Africa is still unknown to most researchers, public health officials and medical practitioners. The objective of this study was to establish the presence and prevalence of pathogenic free-living amoeba (FLA), Cryptosporidium and Giardia in Queen Elizabeth Protected Area (QEPA).Samples were collected from communal taps and natural water sites in QEPA. Physical water parameters were measured in situ. The samples were processed to detect the presence of FLA trophozoites by xenic cultivation, Cryptosporidium oocysts by Ziehl-Neelsen stain and Giardia cysts by Zinc Sulphate floatation technique. Parasites were observed microscopically, identified, counted and recorded. For FLA, genomic DNA was extracted for amplification and sequencing.Both natural and tap water sources were contaminated with FLA, Cryptosporidium spp. and Giardia spp. All protozoan parasites were more abundant in the colder rainy season except for Harmannella spp. and Naegleria spp. which occurred more in the warmer months. The prevalence of all parasites was higher in tap water than in natural water samples. There was a strong negative correlation between the presence of Acanthamoeba spp., Hartmannella spp., Cryptosporidium spp. and Giardia spp. with Dissolved Oxygen (DO) (P < 0.05). The presence of Cryptosporidium spp. showed a significant positive correlation (P < 0.05) with conductivity, pH and Total Dissolved Solids (TDS); whereas the presence of Giardia spp. had only a strong positive correlation with TDS. Molecular genotyping of FLA produced 7 Acanthamoeba, 5 Echinamoeba, 2 Hartmannella, 1 Bodomorpha, 1 Nuclearia and 1 Cercomonas partial sequences.All water collection sites were found to be contaminated with pathogenic protozoa that could possibly be the cause of a number of silent morbidities and mortalities among rural households in QEPA. This implies that water used by communities in QEPA is of poor quality and predisposes them to a variety of protozoan infections including the FLA whose public health importance was never reported, thus necessitating adoption of proper water safety measures."],"affiliation":["Department of Wildlife and Aquatic Animal Resources (WAAR), School of Veterinary Medicine and Animal Resources (SVAR), College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O.Box 7062, Kampala, Uganda. csente37@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Water","Protozoa","Prevalence","Uganda","Free-living","Queen Elizabeth"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4971666?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4971666"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40249-016-0162-5"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-03"],"dateOfRevision":["2016-08-05"],"electronicPublicationDate":["2016-08-03"],"firstPublicationDate":["2016-08-03"],"cprojectID":"PMC4971666"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzD_8VqgcuJwvjr","_score":1,"_source":{"id":["27422403"],"source":["MED"],"pmid":["27422403"],"pmcid":["PMC4947330"],"doi":["10.1186/s12889-016-3191-x"],"title":["Experiences with insecticide-treated curtains: a qualitative study in Iquitos, Peru."],"authorString":["Paz-Soldan VA, Bauer KM, Lenhart A, Cordova Lopez JJ, Elder JP, Scott TW, McCall PJ, Kochel TJ, Morrison AC."],"authorList":[{"author":[{"fullName":["Paz-Soldan VA"],"firstName":["Valerie A"],"lastName":["Paz-Soldan"],"initials":["VA"],"affiliation":["Facultad de Salud Pública y Administración, Universidad Peruana Cayetano Heredia, Lima, Peru. vpazsold@tulane.edu."]},{"fullName":["Bauer KM"],"firstName":["Karin M"],"lastName":["Bauer"],"initials":["KM"],"affiliation":["Department of Global Community Health and Behavioral Sciences, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, Suite 2200, New Orleans, LA, USA."]},{"fullName":["Lenhart A"],"firstName":["Audrey"],"lastName":["Lenhart"],"initials":["A"],"affiliation":["Entomology Branch, Division of Parasitic Diseases and Malaria, United States Centers for Disease Control and Prevention, Atlanta, GA, USA."]},{"fullName":["Cordova Lopez JJ"],"firstName":["Jhonny J"],"lastName":["Cordova Lopez"],"initials":["JJ"],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK."]},{"fullName":["Elder JP"],"firstName":["John P"],"lastName":["Elder"],"initials":["JP"],"affiliation":["Division of Health Promotion and Behavioral Sciences, Graduate School of Public Health, San Diego State University, San Diego, CA, USA."]},{"fullName":["Scott TW"],"firstName":["Thomas W"],"lastName":["Scott"],"initials":["TW"],"affiliation":["Fogarty International Center, National Institutes of Health, Bethesda, MD, USA."]},{"fullName":["McCall PJ"],"firstName":["Philip J"],"lastName":["McCall"],"initials":["PJ"],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK."]},{"fullName":["Kochel TJ"],"firstName":["Tadeusz J"],"lastName":["Kochel"],"initials":["TJ"],"affiliation":["Virology Department, Naval Medical Research Center, Silver Spring, MD, USA."]},{"fullName":["Morrison AC"],"firstName":["Amy C"],"lastName":["Morrison"],"initials":["AC"],"affiliation":["Department of Entomology and Nematology, University of California Davis, Davis, CA, USA."]}]}],"journalInfo":[{"volume":["16"],"journalIssueId":["2401380"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC public health"],"ISOAbbreviation":["BMC Public Health"],"medlineAbbreviation":["BMC Public Health"],"NLMid":["100968562"],"ESSN":["1471-2458"]}]}],"pubYear":["2016"],"pageInfo":["582"],"abstractText":["Dengue is an arthropod-borne viral disease responsible for approximately 400 million infections annually; the only available method of prevention is vector control. It has been previously demonstrated that insecticide treated curtains (ITCs) can lower dengue vector infestations in and around houses. As part of a larger trial examining whether ITCs could reduce dengue transmission in Iquitos, Peru, the objective of this study was to characterize the participants' experience with the ITCs using qualitative methods.Knowledge, attitudes, and practices (KAP) surveys (at baseline, and 9 and 27 months post-ITC distribution, with n = 593, 595 and 511, respectively), focus group discussions (at 6 and 12 months post-ITC distribution, with n = 18 and 33, respectively), and 11 one-on-one interviews (at 12 months post-distribution) were conducted with 605 participants who received ITCs as part of a cluster-randomized trial.Focus groups at 6 months post-ITC distribution revealed that individuals had observed their ITCs to function for approximately 3 months, after which they reported the ITCs were no longer working. Follow up revealed that the ITCs required re-treatment with insecticide at approximately 1 year post-distribution. Over half (55.3 %, n = 329) of participants at 9 months post-ITC distribution and over a third (34.8 %, n = 177) at 27 months post-ITC distribution reported perceiving a decrease in the number of mosquitoes in their home. The percentage of participants who would recommend ITCs to their family or friends in the future remained high throughout the study (94.3 %, n = 561 at 9 months and 94.6 %, n = 488 at 27 months post-distribution). When asked why, participants reported that ITCs were effective at reducing mosquitoes (81.6 and 37.8 %, at 9 and 27 months respectively), that they prevent dengue (5.7 and 51.2 %, at 9 and 27 months), that they are \"beautiful\" (5.9 and 3.1 %), as well as other reasons (6.9 and 2.5 %).ITCs have substantial potential for long term dengue vector control because they are liked by users, both for their perceived effectiveness and for aesthetic reasons, and because they require little proactive behavioral effort on the part of the users. Our results highlight the importance of gathering process (as opposed to outcome) data during vector control studies, without which researchers would not have become aware that the ITCs had lost effectiveness early in the trial."],"affiliation":["Facultad de Salud Pública y Administración, Universidad Peruana Cayetano Heredia, Lima, Peru. vpazsold@tulane.edu."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Qualitative","Dengue","Peru","Insecticide Treated Curtains (Itc)"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947330?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947330"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12889-016-3191-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-16"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-07-16"],"firstPublicationDate":["2016-07-16"],"cprojectID":"PMC4947330"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzP_8VqgcuJwvjv","_score":1,"_source":{"id":["27478378"],"source":["MED"],"pmid":["27478378"],"pmcid":["PMC4951060"],"doi":["10.2147/ndt.s113037"],"title":["Zika virus challenges for neuropsychiatry."],"authorString":["Simões E Silva AC, Moreira JM, Romanelli RM, Teixeira AL."],"authorList":[{"author":[{"fullName":["Simões E Silva AC"],"firstName":["Ana Cristina"],"lastName":["Simões E Silva"],"initials":["AC"],"authorId":[{"_":"0000-0001-9222-3882","$":{"type":"ORCID"}}],"affiliation":["Interdisciplinary Laboratory of Medical Investigation; Department of Pediatrics, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil."]},{"fullName":["Moreira JM"],"firstName":["Janaina Matos"],"lastName":["Moreira"],"initials":["JM"],"affiliation":["Interdisciplinary Laboratory of Medical Investigation; Department of Pediatrics, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil."]},{"fullName":["Romanelli RM"],"firstName":["Roberta Maia Castro"],"lastName":["Romanelli"],"initials":["RM"],"affiliation":["Department of Pediatrics, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil."]},{"fullName":["Teixeira AL"],"firstName":["Antonio Lucio"],"lastName":["Teixeira"],"initials":["AL"],"affiliation":["Interdisciplinary Laboratory of Medical Investigation; Neuropsychiatry Program, Department of Psychiatry and Behavioral Sciences, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9222-3882","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["12"],"journalIssueId":["2363284"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Neuropsychiatric disease and treatment"],"ISOAbbreviation":["Neuropsychiatr Dis Treat"],"medlineAbbreviation":["Neuropsychiatr Dis Treat"],"NLMid":["101240304"],"ISSN":["1176-6328"],"ESSN":["1178-2021"]}]}],"pubYear":["2016"],"pageInfo":["1747-1760"],"abstractText":["Before 2007, Zika virus (ZIKV) was generally considered as an arbovirus of low clinical relevance, causing a mild self-limiting febrile illness in tropical Africa and Southeast Asia. Currently, a large, ongoing outbreak of ZIKV that started in Brazil in 2015 is spreading across the Americas. Virus infection during pregnancy has been potentially linked to congenital malformations, including microcephaly. In addition to congenital malformations, a temporal association between ZIKV infection and an increase in cases of Guillain-Barré syndrome is currently being observed in several countries. The mechanisms underlying these neurological complications are still unknown. Emerging evidence, mainly from in vitro studies, suggests that ZIKV may have direct effects on neuronal cells. The aim of this study was to critically review the literature available regarding the neurobiology of ZIKV and its potential neuropsychiatric manifestations."],"affiliation":["Interdisciplinary Laboratory of Medical Investigation; Department of Pediatrics, Faculty of Medicine, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Microcephaly","Neurodevelopmental Disorders","Guillain-barré Syndrome","Zika Virus"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4951060?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4951060"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.2147/NDT.S113037"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-01"],"dateOfCreation":["2016-08-01"],"dateOfRevision":["2016-08-03"],"electronicPublicationDate":["2016-07-14"],"firstPublicationDate":["2016-07-14"],"cprojectID":"PMC4951060"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrz3_8VqgcuJwvjz","_score":1,"_source":{"id":["27449770"],"source":["MED"],"pmid":["27449770"],"pmcid":["PMC4957883"],"doi":["10.1186/s12941-016-0157-x"],"title":["ChikDenMaZika Syndrome: the challenge of diagnosing arboviral infections in the midst of concurrent epidemics."],"authorString":["Paniz-Mondolfi AE, Rodriguez-Morales AJ, Blohm G, Marquez M, Villamil-Gomez WE."],"authorList":[{"author":[{"fullName":["Paniz-Mondolfi AE"],"firstName":["Alberto E"],"lastName":["Paniz-Mondolfi"],"initials":["AE"],"affiliation":["Laboratory of Biochemistry, Instituto de Biomedicina/IVSS, Caracas, Venezuela."]},{"fullName":["Rodriguez-Morales AJ"],"firstName":["Alfonso J"],"lastName":["Rodriguez-Morales"],"initials":["AJ"],"affiliation":["Committee on Zoonoses and Haemorrhagic Fevers, Asociación Colombiana de Infectología (ACIN), Bogotá, DC, Colombia. arodriguezm@utp.edu.co."]},{"fullName":["Blohm G"],"firstName":["Gabriela"],"lastName":["Blohm"],"initials":["G"],"affiliation":["Department of Biology, University of Florida, Gainesville, FL, USA."]},{"fullName":["Marquez M"],"firstName":["Marilianna"],"lastName":["Marquez"],"initials":["M"],"affiliation":["Department of Pathology and Laboratory Medicine, Hospital Internacional, Barquisimeto, Venezuela."]},{"fullName":["Villamil-Gomez WE"],"firstName":["Wilmer E"],"lastName":["Villamil-Gomez"],"initials":["WE"],"affiliation":["Programa del Doctorado de Medicina Tropical, Universidad del Atlántico, Barranquilla, Atlántico, Colombia."]}]}],"journalInfo":[{"issue":["1"],"volume":["15"],"journalIssueId":["2370323"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Annals of clinical microbiology and antimicrobials"],"ISOAbbreviation":["Ann. Clin. Microbiol. Antimicrob."],"medlineAbbreviation":["Ann Clin Microbiol Antimicrob"],"NLMid":["101152152"],"ESSN":["1476-0711"]}]}],"pubYear":["2016"],"pageInfo":["42"],"affiliation":["Laboratory of Biochemistry, Instituto de Biomedicina/IVSS, Caracas, Venezuela."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957883?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957883"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12941-016-0157-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-25"],"dateOfRevision":["2016-07-29"],"electronicPublicationDate":["2016-07-22"],"firstPublicationDate":["2016-07-22"],"cprojectID":"PMC4957883"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr3hJ9hGurOzZIb3","_score":1,"_source":{"id":["27516967"],"source":["MED"],"pmid":["27516967"],"pmcid":["PMC4976605"],"doi":["10.1016/j.idcr.2016.07.002"],"title":["Co-infection of dengue fever and hepatitis A in a Russian traveler."],"authorString":["Volchkova E, Umbetova K, Belaia O, Sviridova M, Dmitrieva L, Arutyunova D, Chernishov D, Karan L."],"authorList":[{"author":[{"fullName":["Volchkova E"],"firstName":["Elena"],"lastName":["Volchkova"],"initials":["E"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Umbetova K"],"firstName":["Karina"],"lastName":["Umbetova"],"initials":["K"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Belaia O"],"firstName":["Olga"],"lastName":["Belaia"],"initials":["O"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Sviridova M"],"firstName":["Maria"],"lastName":["Sviridova"],"initials":["M"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Dmitrieva L"],"firstName":["Ludmila"],"lastName":["Dmitrieva"],"initials":["L"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Arutyunova D"],"firstName":["Daria"],"lastName":["Arutyunova"],"initials":["D"],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."]},{"fullName":["Chernishov D"],"firstName":["Dmitriy"],"lastName":["Chernishov"],"initials":["D"],"affiliation":["Moscow Infectious Clinical Hospital #2, Moscow, Russia."]},{"fullName":["Karan L"],"firstName":["Ludmila"],"lastName":["Karan"],"initials":["L"],"affiliation":["\"Central Scientific Research Institute of Epidemiology\" Research Institute of Epidemiology, Moscow, Russia."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2422930"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["IDCases"],"ISOAbbreviation":["IDCases"],"medlineAbbreviation":["IDCases"],"NLMid":["101634540"],"ESSN":["2214-2509"]}]}],"pubYear":["2016"],"pageInfo":["67-68"],"abstractText":["We report a hepatitis A (HAV) and dengue virus (DENV) co-infection in Russian man who had been traveling to Dominican Republic. At admission to the hospital hemorrhagic and jaundice symptoms were observed in patient. PCR tests of blood serum and urine revealed RNA dengue virus type 3, HAV RNA, anti-HAV-IgM."],"affiliation":["First Moscow Medical University \"I.M. Sechenov\", Moscow, Russia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Dengue virus","Viral Hepatitis A"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976605?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976605"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.idcr.2016.07.002"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-12"],"dateOfCreation":["2016-08-12"],"dateOfRevision":["2016-08-17"],"electronicPublicationDate":["2016-07-29"],"firstPublicationDate":["2016-07-29"],"cprojectID":"PMC4976605"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr4phOUS2orL1ISN","_score":1,"_source":{"id":["27581122"],"source":["MED"],"pmid":["27581122"],"pmcid":["PMC4981114"],"doi":["10.1590/0074-02760160104"],"title":["Ultrastructure of Zika virus particles in cell cultures."],"authorString":["Barreto-Vieira DF, Barth OM, Silva MA, Santos CC, Santos Ada S, F JB, Filippis AM."],"authorList":[{"author":[{"fullName":["Barreto-Vieira DF"],"firstName":["Debora Ferreira"],"lastName":["Barreto-Vieira"],"initials":["DF"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Barth OM"],"firstName":["Ortrud Monika"],"lastName":["Barth"],"initials":["OM"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Silva MA"],"firstName":["Marcos Alexandre Nunes da"],"lastName":["Silva"],"initials":["MA"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Santos CC"],"firstName":["Carolina Cardoso"],"lastName":["Santos"],"initials":["CC"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Flavivírus, Rio de Janeiro, RJ, Brasil."]},{"fullName":["Santos Ada S"],"firstName":["Aline da Silva"],"lastName":["Santos"],"initials":["Ada S"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Flavivírus, Rio de Janeiro, RJ, Brasil."]},{"fullName":["F JB Filho"],"firstName":["Joaquim Batista"],"lastName":["F"],"initials":["JB"],"affiliation":["Laboratório Central de Saúde Pública do Espírito Santo/Virologia, Vitória, ES, Brasil."]},{"fullName":["Filippis AM"],"firstName":["Ana Maria Bispo de"],"lastName":["Filippis"],"initials":["AM"],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Flavivírus, Rio de Janeiro, RJ, Brasil."]}]}],"journalInfo":[{"issue":["8"],"volume":["111"],"journalIssueId":["2440076"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["532-534"],"abstractText":["Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient's blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible."],"affiliation":["Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Morfologia e Morfogênese Viral, Rio de Janeiro, RJ, Brasil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981114?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4981114"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160104"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-01"],"dateOfRevision":["2016-09-04"],"electronicPublicationDate":["2016-07-11"],"firstPublicationDate":["2016-07-11"],"cprojectID":"PMC4981114"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr7iJ9hGurOzZIcI","_score":1,"_source":{"id":["27503033"],"source":["MED"],"pmid":["27503033"],"pmcid":["PMC4989439"],"doi":["10.4103/0366-6999.187864"],"title":["First Case of Laboratory-confirmed Zika Virus Infection Imported into China."],"authorString":["Zhong YB, Liu XQ, Deng YC, Xu PH, Zhong GR, Zhang W."],"authorList":[{"author":[{"fullName":["Zhong YB"],"firstName":["Yuan-Bin"],"lastName":["Zhong"],"initials":["YB"],"affiliation":["Department of Infectious Disease and Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China."]},{"fullName":["Liu XQ"],"firstName":["Xiao-Qing"],"lastName":["Liu"],"initials":["XQ"],"affiliation":["Jiangxi Province Center for Disease Control and Prevention, Nanchang, Jiangxi 330029, China."]},{"fullName":["Deng YC"],"firstName":["Yi-Chu"],"lastName":["Deng"],"initials":["YC"],"affiliation":["Department of Infectious Disease, Ganxian People's Hospital of Ganzhou, Ganxian, Jiangxi 341100, China."]},{"fullName":["Xu PH"],"firstName":["Ping-Hua"],"lastName":["Xu"],"initials":["PH"],"affiliation":["Department of Infectious Disease, Ganxian People's Hospital of Ganzhou, Ganxian, Jiangxi 341100, China."]},{"fullName":["Zhong GR"],"firstName":["Gong-Rong"],"lastName":["Zhong"],"initials":["GR"],"affiliation":["Department of Infectious Disease, Ganxian People's Hospital of Ganzhou, Ganxian, Jiangxi 341100, China."]},{"fullName":["Zhang W"],"firstName":["Wei"],"lastName":["Zhang"],"initials":["W"],"affiliation":["Department of Infectious Disease and Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China."]}]}],"journalInfo":[{"issue":["16"],"volume":["129"],"journalIssueId":["2444062"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Chinese medical journal"],"ISOAbbreviation":["Chin. Med. J."],"medlineAbbreviation":["Chin Med J (Engl)"],"NLMid":["7513795"],"ISSN":["0366-6999"]}]}],"pubYear":["2016"],"pageInfo":["2013-2014"],"affiliation":["Department of Infectious Disease and Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4989439"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/0366-6999.187864"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-09"],"dateOfRevision":["2016-09-09"],"firstPublicationDate":["2016-01-01"],"cprojectID":"PMC4989439"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr7xhOUS2orL1ISV","_score":1,"_source":{"id":["27532497"],"source":["MED"],"pmid":["27532497"],"pmcid":["PMC4988764"],"doi":["10.1371/journal.pone.0160386"],"title":["Design and Testing of Novel Lethal Ovitrap to Reduce Populations of Aedes Mosquitoes: Community-Based Participatory Research between Industry, Academia and Communities in Peru and Thailand."],"authorString":["Paz-Soldan VA, Yukich J, Soonthorndhada A, Giron M, Apperson CS, Ponnusamy L, Schal C, Morrison AC, Keating J, Wesson DM."],"authorList":[{"author":[{"fullName":["Paz-Soldan VA"],"firstName":["Valerie A"],"lastName":["Paz-Soldan"],"initials":["VA"],"authorId":[{"_":"0000-0002-1065-4919","$":{"type":"ORCID"}}],"affiliation":["Center for Applied Malaria Research and Evaluation, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, New Orleans, LA 70112, United States of America."]},{"fullName":["Yukich J"],"firstName":["Josh"],"lastName":["Yukich"],"initials":["J"],"affiliation":["Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, New Orleans, LA 70112, United States of America."]},{"fullName":["Soonthorndhada A"],"firstName":["Amara"],"lastName":["Soonthorndhada"],"initials":["A"],"affiliation":["Institute for Population and Social Research, Mahidol University, Salaya, Phuthamontol, Nakornpathom, Thailand, 73170."]},{"fullName":["Giron M"],"firstName":["Maziel"],"lastName":["Giron"],"initials":["M"],"affiliation":["Department of Health Science and Recreation, San Jose State University, 1 Washington Sq, San Jose, CA 95192, United States of America."]},{"fullName":["Apperson CS"],"firstName":["Charles S"],"lastName":["Apperson"],"initials":["CS"],"affiliation":["Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695, United States of America."]},{"fullName":["Ponnusamy L"],"firstName":["Loganathan"],"lastName":["Ponnusamy"],"initials":["L"],"affiliation":["Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695, United States of America."]},{"fullName":["Schal C"],"firstName":["Coby"],"lastName":["Schal"],"initials":["C"],"affiliation":["Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27695, United States of America."]},{"fullName":["Morrison AC"],"firstName":["Amy C"],"lastName":["Morrison"],"initials":["AC"],"affiliation":["Department of Entomology and Nematology, University of California Davis, One Shields Ave, Davis, CA 95616, United States of America."]},{"fullName":["Keating J"],"firstName":["Joseph"],"lastName":["Keating"],"initials":["J"],"affiliation":["Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, New Orleans, LA 70112, United States of America."]},{"fullName":["Wesson DM"],"firstName":["Dawn M"],"lastName":["Wesson"],"initials":["DM"],"affiliation":["Vector Borne Infectious Disease Research Center, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, New Orleans, LA 70112, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1065-4919","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["8"],"volume":["11"],"journalIssueId":["2435494"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0160386"],"abstractText":["Dengue virus (and Chikungunya and Zika viruses) is transmitted by Aedes aegypti and Aedes albopictus mosquitoes and causes considerable human morbidity and mortality. As there is currently no vaccine or chemoprophylaxis to protect people from dengue virus infection, vector control is the only viable option for disease prevention. The purpose of this paper is to illustrate the design and placement process for an attractive lethal ovitrap to reduce vector populations and to describe lessons learned in the development of the trap.This study was conducted in 2010 in Iquitos, Peru and Lopburi Province, Thailand and used an iterative community-based participatory approach to adjust design specifications of the trap, based on community members' perceptions and feedback, entomological findings in the lab, and design and research team observations. Multiple focus group discussions (FGD) were held over a 6 month period, stratified by age, sex and motherhood status, to inform the design process. Trap testing transitioned from the lab to within households.Through an iterative process of working with specifications from the research team, findings from the laboratory testing, and feedback from FGD, the design team narrowed trap design options from 22 to 6. Comments from the FGD centered on safety for children and pets interacting with traps, durability, maintenance issues, and aesthetics. Testing in the laboratory involved releasing groups of 50 gravid Ae. aegypti in walk-in rooms and assessing what percentage were caught in traps of different colors, with different trap cover sizes, and placed under lighter or darker locations. Two final trap models were mocked up and tested in homes for a week; one model was the top choice in both Iquitos and Lopburi.The community-based participatory process was essential for the development of novel traps that provided effective vector control, but also met the needs and concerns of community members."],"affiliation":["Center for Applied Malaria Research and Evaluation, Tulane University School of Public Health and Tropical Medicine, 1440 Canal Street, New Orleans, LA 70112, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988764?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988764"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0160386"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-18"],"dateOfRevision":["2016-08-31"],"electronicPublicationDate":["2016-08-17"],"firstPublicationDate":["2016-08-17"],"cprojectID":"PMC4988764"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr8mhOUS2orL1ISa","_score":1,"_source":{"id":["27555980"],"source":["MED"],"pmid":["27555980"],"pmcid":["PMC4993616"],"doi":["10.1055/s-0036-1587323"],"title":["Congenital Chikungunya Virus Infection after an Outbreak in Salvador, Bahia, Brazil."],"authorString":["Lyra PP, Campos GS, Bandeira ID, Sardi SI, Costa LF, Santos FR, Ribeiro CA, Jardim AM, Santiago AC, de Oliveira PM, Moreira LM."],"authorList":[{"author":[{"fullName":["Lyra PP"],"firstName":["Priscila Pinheiro Ribeiro"],"lastName":["Lyra"],"initials":["PP"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["Campos GS"],"firstName":["Gúbio Soares"],"lastName":["Campos"],"initials":["GS"],"affiliation":["Laboratory of Virology, Health Science Institute, Federal University of Bahia, Bahia, Brazil."]},{"fullName":["Bandeira ID"],"firstName":["Igor Dórea"],"lastName":["Bandeira"],"initials":["ID"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["Sardi SI"],"firstName":["Silvia Ines"],"lastName":["Sardi"],"initials":["SI"],"affiliation":["Laboratory of Virology, Health Science Institute, Federal University of Bahia, Bahia, Brazil."]},{"fullName":["Costa LF"],"firstName":["Lilian Ferreira de Moura"],"lastName":["Costa"],"initials":["LF"],"affiliation":["Laboratory of Virology, Health Science Institute, Federal University of Bahia, Bahia, Brazil."]},{"fullName":["Santos FR"],"firstName":["Flávia Rocha"],"lastName":["Santos"],"initials":["FR"],"affiliation":["Laboratory of Virology, Health Science Institute, Federal University of Bahia, Bahia, Brazil."]},{"fullName":["Ribeiro CA"],"firstName":["Carlos Alexandre Santos"],"lastName":["Ribeiro"],"initials":["CA"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["Jardim AM"],"firstName":["Alena Maria Barreto"],"lastName":["Jardim"],"initials":["AM"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["Santiago AC"],"firstName":["Ana Cecília Travassos"],"lastName":["Santiago"],"initials":["AC"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["de Oliveira PM"],"firstName":["Patrícia Maria Ribeiro"],"lastName":["de Oliveira"],"initials":["PM"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]},{"fullName":["Moreira LM"],"firstName":["Lícia Maria Oliveira"],"lastName":["Moreira"],"initials":["LM"],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."]}]}],"journalInfo":[{"issue":["3"],"volume":["6"],"journalIssueId":["2434529"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["AJP reports"],"ISOAbbreviation":["AJP Rep"],"medlineAbbreviation":["AJP Rep"],"NLMid":["101569862"],"ISSN":["2157-6998"],"ESSN":["2157-7005"]}]}],"pubYear":["2016"],"pageInfo":["e299-300"],"abstractText":["There is little information about the congenital chikungunya virus (CHIKV) transmission. We describe two cases of well-documented congenital CHIKV infection in Salvador-Brazil, where CHIKV has been identified since 2014. The outbreak in the city led to the clinical CHIKV diagnoses of both pregnant women 2 days before delivery. Urine and blood samples from the mothers and newborns were collected and tested for reverse transcription-polymerase chain reaction (PCR) analysis for Zika, dengue, and CHIKV. Both neonates and mothers had positive urine and serum PCR results for CHIKV. The newborns had significant perinatal complications and were admitted to the neonatal intensive care unit. The purpose of our case report is to show how severe congenital CHIKV infection can be and the importance to include CHIKV infection in the differential diagnosis of neonatal sepsis when mothers have clinical signs of the disease and live in an affected area."],"affiliation":["Department of Pediatrics, Climério de Oliveira Maternity, Federal University of Bahia School of Medicine, Bahia, Brazil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Infection","Newborn","congenital","Chikungunya"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4993616?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4993616"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1055/s-0036-1587323"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-24"],"dateOfCreation":["2016-08-24"],"dateOfRevision":["2016-08-26"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4993616"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsAYJ9hGurOzZIcS","_score":1,"_source":{"id":["27015222"],"source":["MED"],"pmid":["27015222"],"pmcid":["PMC4998417"],"doi":["10.1097/md.0000000000003201"],"title":["Investigation Into an Outbreak of Dengue-like Illness in Pernambuco, Brazil, Revealed a Cocirculation of Zika, Chikungunya, and Dengue Virus Type 1."],"authorString":["Pessôa R, Patriota JV, Lourdes de Souza Md, Felix AC, Mamede N, Sanabani SS."],"authorList":[{"author":[{"fullName":["Pessôa R"],"firstName":["Rodrigo"],"lastName":["Pessôa"],"initials":["R"],"affiliation":["From the Clinical Laboratory, Department of Pathology (RP, SSS), Hospital das Clínicas (HC), School of Medicine, University of São Paulo, São Paulo; Municipal Hospital of Tuparetama (JVP, MDLDS, ACF, NM), Pernambuco; and Department of Virology (SSS), São Paulo Institute of Tropical Medicine, São Paulo, Brazil."]},{"fullName":["Patriota JV"],"firstName":["João Veras"],"lastName":["Patriota"],"initials":["JV"]},{"fullName":["Lourdes de Souza Md"],"firstName":["Maria de"],"lastName":["Lourdes de Souza"],"initials":["Md"]},{"fullName":["Felix AC"],"firstName":["Alvina Clara"],"lastName":["Felix"],"initials":["AC"]},{"fullName":["Mamede N"],"firstName":["Nubia"],"lastName":["Mamede"],"initials":["N"]},{"fullName":["Sanabani SS"],"firstName":["Sabri S"],"lastName":["Sanabani"],"initials":["SS"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-8876-8262","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["12"],"volume":["95"],"journalIssueId":["2390573"],"dateOfPublication":["2016 Mar"],"monthOfPublication":["3"],"yearOfPublication":["2016"],"printPublicationDate":["2016-03-01"],"journal":[{"title":["Medicine"],"ISOAbbreviation":["Medicine (Baltimore)"],"medlineAbbreviation":["Medicine (Baltimore)"],"NLMid":["2985248R"],"ISSN":["0025-7974"],"ESSN":["1536-5964"]}]}],"pubYear":["2016"],"pageInfo":["e3201"],"abstractText":["In April 2015, an outbreak of dengue-like illness occurred in Tuparetama, a small city in the northeast region of Brazil; this outbreak was characterized by its fast expansion. An investigation was initiated to identify the viral etiologies and advise the health authorities on implementing control measures to contain the outbreak. This is the first report of this outbreak in the northeast, even though a few cases were documented earlier in a neighboring city.Plasma samples were obtained from 77 suspected dengue patients attending the main hospital in the city. Laboratory assays, such as real-time reverse transcription polymerase chain reaction, virus cDNA sequencing, and enzyme-linked immunosorbent assay, were employed to identify the infecting virus and molecular phylogenetic analysis was performed to define the circulating viral genotypes.RNA of Zika virus (ZIKV) and Dengue virus (DENV) or IgM antibodies (Abs) to DENV or chikungunya (CHIKV) were detected in 40 of the 77 plasma samples (51.9%). DENV was found in 9 patients (11.7%), ZIKV was found in 31 patients (40.2%), CHIKV in 1 patient (1.3%), and coinfection of DENV and ZIKV was detected in 2 patients (2.6%). The phylogenetic analysis of 2 available partial DENV and 14 ZIKV sequences revealed the identities of genotype 1 and the Asiatic lineage, respectively.Consistent with recent reports from the same region, our results showed that the ongoing outbreak is caused by ZIKV, DENV, and CHIKV. This emphasizes the need for a routine and differential diagnosis of arboviruses in patients with dengue-like illness. Coordinated efforts are necessary to contain the outbreak. Continued surveillance will be important to assess the effectiveness of current and future prevention strategies."],"affiliation":["From the Clinical Laboratory, Department of Pathology (RP, SSS), Hospital das Clínicas (HC), School of Medicine, University of São Paulo, São Paulo; Municipal Hospital of Tuparetama (JVP, MDLDS, ACF, NM), Pernambuco; and Department of Virology (SSS), São Paulo Institute of Tropical Medicine, São Paulo, Brazil."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Diagnosis, Differential"]},{"majorTopic_YN":["N"],"descriptorName":["Cross-Sectional Studies"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Genotype"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Aged, 80 and over"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Child"]},{"majorTopic_YN":["N"],"descriptorName":["Child, Preschool"]},{"majorTopic_YN":["N"],"descriptorName":["Infant"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Chikungunya Fever"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4998417?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4998417"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1097/MD.0000000000003201"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-08-03"],"dateOfCreation":["2016-03-26"],"dateOfRevision":["2016-09-04"],"firstPublicationDate":["2016-03-01"],"cprojectID":"PMC4998417"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsBUJ9hGurOzZIcU","_score":1,"_source":{"id":["27571201"],"source":["MED"],"pmid":["27571201"],"pmcid":["PMC5003370"],"doi":["10.1371/journal.pntd.0004887"],"title":["Development of 2, 7-Diamino-1, 8-Naphthyridine (DANP) Anchored Hairpin Primers for RT-PCR Detection of Chikungunya Virus Infection."],"authorString":["Chen H, Parimelalagan M, Takei F, Hapuarachchi HC, Koay ES, Ng LC, Ho PS, Nakatani K, Chu JJ."],"authorList":[{"author":[{"fullName":["Chen H"],"firstName":["Huixin"],"lastName":["Chen"],"initials":["H"],"affiliation":["Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore."]},{"fullName":["Parimelalagan M"],"firstName":["Mariya"],"lastName":["Parimelalagan"],"initials":["M"],"affiliation":["School of Applied Science, Republic Polytechnic, Singapore."]},{"fullName":["Takei F"],"firstName":["Fumie"],"lastName":["Takei"],"initials":["F"],"affiliation":["The National Defense Medical College, Tokorozawa, Saitama, Japan."]},{"fullName":["Hapuarachchi HC"],"firstName":["Hapuarachchige Chanditha"],"lastName":["Hapuarachchi"],"initials":["HC"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore."]},{"fullName":["Koay ES"],"firstName":["Evelyn Siew-Chuan"],"lastName":["Koay"],"initials":["ES"],"affiliation":["Molecular Diagnosis Centre, Department of Laboratory Medicine and Pathology, Yong Loo Lin School of Medicine, National University Health System, Level 3, National University Hospital Main Building, Singapore."]},{"fullName":["Ng LC"],"firstName":["Lee Ching"],"lastName":["Ng"],"initials":["LC"],"affiliation":["Environmental Health Institute, National Environment Agency, Singapore."]},{"fullName":["Ho PS"],"firstName":["Phui San"],"lastName":["Ho"],"initials":["PS"],"affiliation":["School of Applied Science, Republic Polytechnic, Singapore."]},{"fullName":["Nakatani K"],"firstName":["Kazuhiko"],"lastName":["Nakatani"],"initials":["K"],"affiliation":["The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan."]},{"fullName":["Chu JJ"],"firstName":["Justin Jang Hann"],"lastName":["Chu"],"initials":["JJ"],"affiliation":["Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004887"],"abstractText":["A molecular diagnostic platform with DANP-anchored hairpin primer was developed and evaluated for the rapid and cost-effective detection of Chikungunya virus (CHIKV) with high sensitivity and specificity. The molecule 2, 7-diamino-1, 8-naphthyridine (DANP) binds to a cytosine-bulge and emits fluorescence at 450 nm when it is excited by 400 nm light. Thus, by measuring the decline in fluorescence emitted from DANP-primer complexes after PCR reaction, we could monitor the PCR progress. By adapting this property of DANP, we have previously developed the first generation DANP-coupled hairpin RT-PCR assay. In the current study, we improved the assay performance by conjugating the DANP molecule covalently onto the hairpin primer to fix the DANP/primer ratio at 1:1; and adjusting the excitation emission wavelength to 365/430 nm to minimize the background signal and a 'turn-on' system is achieved. After optimizing the PCR cycle number to 30, we not only shortened the total assay turnaround time to 60 minutes, but also further reduced the background fluorescence. The detection limit of our assay was 0.001 PFU per reaction. The DANP-anchored hairpin primer, targeting nsP2 gene of CHIKV genome, is highly specific to CHIKV, having no cross-reactivity to a panel of other RNA viruses tested. In conclusion, we report here a molecular diagnostic assay that is sensitive, specific, rapid and cost effective for CHIKV detection and can be performed where no real time PCR instrumentation is required. Our results from patient samples indicated 93.62% sensitivity and 100% specificity of this method, ensuring that it can be a useful tool for rapid detection of CHIKV for outbreaks in many parts of the world."],"affiliation":["Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5003370?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5003370"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004887"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-30"],"electronicPublicationDate":["2016-08-29"],"firstPublicationDate":["2016-08-29"],"cprojectID":"PMC5003370"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsIV_8VqgcuJwvkt","_score":1,"_source":{"id":["27610227"],"source":["MED"],"pmid":["27610227"],"pmcid":["PMC4995683"],"doi":["10.12688/f1000research.8766.1"],"title":["The rise and fall of infectious disease in a warmer world."],"authorString":["Lafferty KD, Mordecai EA."],"authorList":[{"author":[{"fullName":["Lafferty KD"],"firstName":["Kevin D"],"lastName":["Lafferty"],"initials":["KD"],"affiliation":["Western Ecological Research Center, U.S. Geological Survey at Marine Science Institute, University of California, Santa Barbara, CA, 93106, USA."]},{"fullName":["Mordecai EA"],"firstName":["Erin A"],"lastName":["Mordecai"],"initials":["EA"],"affiliation":["Department of Biology, Stanford University, Stanford, CA, 94305, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"abstractText":["Now-outdated estimates proposed that climate change should have increased the number of people at risk of malaria, yet malaria and several other infectious diseases have declined. Although some diseases have increased as the climate has warmed, evidence for widespread climate-driven disease expansion has not materialized, despite increased research attention. Biological responses to warming depend on the non-linear relationships between physiological performance and temperature, called the thermal response curve. This leads performance to rise and fall with temperature. Under climate change, host species and their associated parasites face extinction if they cannot either thermoregulate or adapt by shifting phenology or geographic range. Climate change might also affect disease transmission through increases or decreases in host susceptibility and infective stage (and vector) production, longevity, and pathology. Many other factors drive disease transmission, especially economics, and some change in time along with temperature, making it hard to distinguish whether temperature drives disease or just correlates with disease drivers. Although it is difficult to predict how climate change will affect infectious disease, an ecological approach can help meet the challenge."],"affiliation":["Western Ecological Research Center, U.S. Geological Survey at Marine Science Institute, University of California, Santa Barbara, CA, 93106, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Ecology","Climate change","Infectious disease"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4995683?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4995683"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.12688/f1000research.8766.1"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-09-09"],"dateOfCreation":["2016-09-09"],"electronicPublicationDate":["2016-08-19"],"firstPublicationDate":["2016-08-19"],"cprojectID":"PMC4995683"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrbGhOUS2orL1IQh","_score":1,"_source":{"id":["27280061"],"source":["MED"],"pmid":["27280061"],"pmcid":["PMC4883055"],"doi":["10.1371/currents.outbreaks.a832cf06c4bf89fb2e15cb29d374f9de"],"title":["Zika Virus, Elevation, and Transmission Risk."],"authorString":["Peterson AT, Osorio J, Qiao H, Escobar LE."],"authorList":[{"author":[{"fullName":["Peterson AT"],"firstName":["A Townsend"],"lastName":["Peterson"],"initials":["AT"],"affiliation":["Biodiversity Institute, University of Kansas, Lawrence, Kansas, USA."]},{"fullName":["Osorio J"],"firstName":["Jorge"],"lastName":["Osorio"],"initials":["J"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA."]},{"fullName":["Qiao H"],"firstName":["Huijie"],"lastName":["Qiao"],"initials":["H"],"affiliation":["Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China."]},{"fullName":["Escobar LE"],"firstName":["Luis E"],"lastName":["Escobar"],"initials":["LE"],"affiliation":["Minnesota Aquatic Invasive Species Research Center and Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, USA."]}]}],"journalInfo":[{"volume":["8"],"journalIssueId":["2367947"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PLoS currents"],"ISOAbbreviation":["PLoS Curr"],"medlineAbbreviation":["PLoS Curr"],"NLMid":["101515638"],"ESSN":["2157-3999"]}]}],"pubYear":["2016"],"abstractText":["Zika virus has appeared in the Americas in the form of a major outbreak, and is now known to cause birth defects when pregnant women are infected. As a result, the Centers for Disease Control and Prevention issued travel guidelines, in the form of an elevational risk definition: destinations below 2000m are considered as at-risk.We explored the distribution of known Zika virus vector mosquito species in relation to climatic conditions, elevation, latitude, and air traffic connections to the United States.In view of the tropical and subtropical nature of the mosquito species that are the primary Zika virus vectors, we point out that climate varies rather dramatically with respect to elevation and latitude, such that a single elevational criterion will be a poor predictor of potential for transmission.We suggest an initial adjustment would consider latitude in addition to elevation; a more definitive, quantitative analysis of risk would consider variables of ecology, climate, human condition, and connectivity of areas."],"affiliation":["Biodiversity Institute, University of Kansas, Lawrence, Kansas, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","Climate","Disease Outbreak","Zika","Travel Advisory"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4883055"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/currents.outbreaks.a832cf06c4bf89fb2e15cb29d374f9de"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-09"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-12"],"electronicPublicationDate":["2016-05-09"],"firstPublicationDate":["2016-05-09"],"cprojectID":"PMC4883055"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnreR_8VqgcuJwvih","_score":1,"_source":{"id":["27223865"],"source":["MED"],"pmid":["27223865"],"pmcid":["PMC4878296"],"doi":["10.1590/0074-02760160001"],"title":["Emerging infectious disease and fast-track publication: when public health gets priority over the formality of scholarly publishing."],"authorString":["Pirmez C, Brandão AA, Momen H."],"authorList":[{"author":[{"fullName":["Pirmez C"],"firstName":["Claude"],"lastName":["Pirmez"],"initials":["C"]},{"fullName":["Brandão AA"],"firstName":["Adeilton Alves"],"lastName":["Brandão"],"initials":["AA"]},{"fullName":["Momen H"],"firstName":["Hooman"],"lastName":["Momen"],"initials":["H"]}]}],"journalInfo":[{"issue":["5"],"volume":["111"],"journalIssueId":["2412036"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2016"],"pageInfo":["285"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878296?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4878296"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760160001"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-26"],"dateOfRevision":["2016-05-27"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4878296"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnre9hOUS2orL1IQr","_score":1,"_source":{"id":["27274912"],"source":["MED"],"pmid":["27274912"],"pmcid":["PMC4892191"],"doi":["10.1089/biores.2016.0010"],"title":["Microbiota and Neurological Disorders: A Gut Feeling."],"authorString":["Moos WH, Faller DV, Harpp DN, Kanara I, Pernokas J, Powers WR, Steliou K."],"authorList":[{"author":[{"fullName":["Moos WH"],"firstName":["Walter H"],"lastName":["Moos"],"initials":["WH"],"affiliation":["Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco , San Francisco, California."]},{"fullName":["Faller DV"],"firstName":["Douglas V"],"lastName":["Faller"],"initials":["DV"],"affiliation":["Department of Medicine, Boston University School of Medicine, Boston, Massachusetts.; Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts."]},{"fullName":["Harpp DN"],"firstName":["David N"],"lastName":["Harpp"],"initials":["DN"],"affiliation":["Department of Chemistry, McGill University , Montreal, Canada ."]},{"fullName":["Kanara I"],"firstName":["Iphigenia"],"lastName":["Kanara"],"initials":["I"],"affiliation":["Weatherhead Center for International Affairs, Harvard University, Cambridge, Massachusetts.; Consulate General of Greece in Boston, Boston, Massachusetts."]},{"fullName":["Pernokas J"],"firstName":["Julie"],"lastName":["Pernokas"],"initials":["J"],"affiliation":["Advanced Dental Associates of New England, Woburn, Massachusetts."]},{"fullName":["Powers WR"],"firstName":["Whitney R"],"lastName":["Powers"],"initials":["WR"],"affiliation":["Department of Health Sciences, Boston University, Boston, Massachusetts.; Department of Anatomy, Boston University School of Medicine, Boston, Massachusetts."]},{"fullName":["Steliou K"],"firstName":["Kosta"],"lastName":["Steliou"],"initials":["K"],"affiliation":["Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts.; PhenoMatriX, Inc., Boston, Massachusetts."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2373829"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BioResearch open access"],"ISOAbbreviation":["Biores Open Access"],"medlineAbbreviation":["Biores Open Access"],"NLMid":["101579333"],"ISSN":["2164-7844"],"ESSN":["2164-7860"]}]}],"pubYear":["2016"],"pageInfo":["137-145"],"abstractText":["In the past century, noncommunicable diseases have surpassed infectious diseases as the principal cause of sickness and death, worldwide. Trillions of commensal microbes live in and on our body, and constitute the human microbiome. The vast majority of these microorganisms are maternally derived and live in the gut, where they perform functions essential to our health and survival, including: digesting food, activating certain drugs, producing short-chain fatty acids (which help to modulate gene expression by inhibiting the deacetylation of histone proteins), generating anti-inflammatory substances, and playing a fundamental role in the induction, training, and function of our immune system. Among the many roles the microbiome ultimately plays, it mitigates against untoward effects from our exposure to the environment by forming a biotic shield between us and the outside world. The importance of physical activity coupled with a balanced and healthy diet in the maintenance of our well-being has been recognized since antiquity. However, it is only recently that characterization of the host-microbiome intermetabolic and crosstalk pathways has come to the forefront in studying therapeutic design. As reviewed in this report, synthetic biology shows potential in developing microorganisms for correcting pathogenic dysbiosis (gut microbiota-host maladaptation), although this has yet to be proven. However, the development and use of small molecule drugs have a long and successful history in the clinic, with small molecule histone deacetylase inhibitors representing one relevant example already approved to treat cancer and other disorders. Moreover, preclinical research suggests that epigenetic treatment of neurological conditions holds significant promise. With the mouth being an extension of the digestive tract, it presents a readily accessible diagnostic site for the early detection of potential unhealthy pathogens resident in the gut. Taken together, the data outlined herein provide an encouraging roadmap toward important new medicines and companion diagnostic platforms in a wide range of therapeutic indications."],"affiliation":["Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco , San Francisco, California."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Fatty acids","Mitochondria","Immune system","Schizophrenia","Histone deacetylase","Exercise","Probiotics","Microbiota","Dementia","Autism","Epigenetic","Dysbiosis","Synthetic Biology","Neurodegenerative","Α-lipoic Acid"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4892191?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4892191"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1089/biores.2016.0010"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-09"],"dateOfCreation":["2016-06-09"],"dateOfRevision":["2016-06-10"],"electronicPublicationDate":["2016-05-01"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4892191"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrgDhOUS2orL1IQz","_score":1,"_source":{"id":["27156023"],"source":["MED"],"pmid":["27156023"],"pmcid":["PMC4906366"],"doi":["10.1016/j.chom.2016.04.021"],"title":["Wolbachia Blocks Currently Circulating Zika Virus Isolates in Brazilian Aedes aegypti Mosquitoes."],"authorString":["Dutra HL, Rocha MN, Dias FB, Mansur SB, Caragata EP, Moreira LA."],"authorList":[{"author":[{"fullName":["Dutra HL"],"firstName":["Heverton Leandro Carneiro"],"lastName":["Dutra"],"initials":["HL"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil."]},{"fullName":["Rocha MN"],"firstName":["Marcele Neves"],"lastName":["Rocha"],"initials":["MN"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil."]},{"fullName":["Dias FB"],"firstName":["Fernando Braga Stehling"],"lastName":["Dias"],"initials":["FB"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil; Plataforma de Vetores de Doenças-Fiocruz, CE, 60190-800, Brazil."]},{"fullName":["Mansur SB"],"firstName":["Simone Brutman"],"lastName":["Mansur"],"initials":["SB"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil."]},{"fullName":["Caragata EP"],"firstName":["Eric Pearce"],"lastName":["Caragata"],"initials":["EP"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil."]},{"fullName":["Moreira LA"],"firstName":["Luciano Andrade"],"lastName":["Moreira"],"initials":["LA"],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil. Electronic address: luciano@cpqrr.fiocruz.br."]}]}],"journalInfo":[{"issue":["6"],"volume":["19"],"journalIssueId":["2418140"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Cell host & microbe"],"ISOAbbreviation":["Cell Host Microbe"],"medlineAbbreviation":["Cell Host Microbe"],"NLMid":["101302316"],"ISSN":["1931-3128"],"ESSN":["1934-6069"]}]}],"pubYear":["2016"],"pageInfo":["771-774"],"abstractText":["The recent association of Zika virus with cases of microcephaly has sparked a global health crisis and highlighted the need for mechanisms to combat the Zika vector, Aedes aegypti mosquitoes. Wolbachia pipientis, a bacterial endosymbiont of insect, has recently garnered attention as a mechanism for arbovirus control. Here we report that Aedes aegypti harboring Wolbachia are highly resistant to infection with two currently circulating Zika virus isolates from the recent Brazilian epidemic. Wolbachia-harboring mosquitoes displayed lower viral prevalence and intensity and decreased disseminated infection and, critically, did not carry infectious virus in the saliva, suggesting that viral transmission was blocked. Our data indicate that the use of Wolbachia-harboring mosquitoes could represent an effective mechanism to reduce Zika virus transmission and should be included as part of Zika control strategies."],"affiliation":["Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor, Centro de Pesquisas René Rachou-Fiocruz, Belo Horizonte, MG, 30190-002, Brazil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4906366?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4906366"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.chom.2016.04.021"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["2"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-10"],"dateOfRevision":["2016-06-23"],"electronicPublicationDate":["2016-05-04"],"firstPublicationDate":["2016-05-03"],"cprojectID":"PMC4906366"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrgahOUS2orL1IQ5","_score":1,"_source":{"id":["27310141"],"source":["MED"],"pmid":["27310141"],"pmcid":["PMC4911004"],"doi":["10.1371/journal.ppat.1005676"],"title":["Mosquito Saliva Increases Endothelial Permeability in the Skin, Immune Cell Migration, and Dengue Pathogenesis during Antibody-Dependent Enhancement."],"authorString":["Schmid MA, Glasner DR, Shah S, Michlmayr D, Kramer LD, Harris E."],"authorList":[{"author":[{"fullName":["Schmid MA"],"firstName":["Michael A"],"lastName":["Schmid"],"initials":["MA"],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Glasner DR"],"firstName":["Dustin R"],"lastName":["Glasner"],"initials":["DR"],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Shah S"],"firstName":["Sanjana"],"lastName":["Shah"],"initials":["S"],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Michlmayr D"],"firstName":["Daniela"],"lastName":["Michlmayr"],"initials":["D"],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."]},{"fullName":["Kramer LD"],"firstName":["Laura D"],"lastName":["Kramer"],"initials":["LD"],"affiliation":["Wadsworth Center, New York State Department of Health, Albany, New York, United States of America."]},{"fullName":["Harris E"],"firstName":["Eva"],"lastName":["Harris"],"initials":["E"],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."]}]}],"journalInfo":[{"issue":["6"],"volume":["12"],"journalIssueId":["2413990"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS pathogens"],"ISOAbbreviation":["PLoS Pathog."],"medlineAbbreviation":["PLoS Pathog"],"NLMid":["101238921"],"ISSN":["1553-7366"],"ESSN":["1553-7374"]}]}],"pubYear":["2016"],"pageInfo":["e1005676"],"abstractText":["Dengue remains the most prevalent arthropod-borne viral disease in humans. While probing for blood vessels, Aedes aegypti and Ae. albopictus mosquitoes transmit the four serotypes of dengue virus (DENV1-4) by injecting virus-containing saliva into the skin. Even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. Inoculating mice lacking the interferon-α/β receptor intradermally with DENV revealed that mosquito salivary gland extract (SGE) exacerbates dengue pathogenesis specifically in the presence of enhancing serotype-cross-reactive antibodies-when individuals already carry an increased risk for severe disease. We further establish that SGE increases viral titers in the skin, boosts antibody-enhanced DENV infection of dendritic cells and macrophages in the dermis, and amplifies dendritic cell migration to skin-draining lymph nodes. We demonstrate that SGE directly disrupts endothelial barrier function in vitro and induces endothelial permeability in vivo in the skin. Finally, we show that surgically removing the site of DENV transmission in the skin after 4 hours rescued mice from disease in the absence of SGE, but no longer prevented lethal antibody-enhanced disease when SGE was present. These results indicate that SGE accelerates the dynamics of dengue pathogenesis after virus transmission in the skin and induces severe antibody-enhanced disease systemically. Our study reveals novel aspects of dengue pathogenesis and suggests that animal models of dengue and pre-clinical testing of dengue vaccines should consider mosquito-derived factors as well as enhancing antibodies."],"affiliation":["Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4911004?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4911004"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.ppat.1005676"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4911004"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrhIJ9hGurOzZIan","_score":1,"_source":{"id":["27366163"],"source":["MED"],"pmid":["27366163"],"pmcid":["PMC4904578"],"doi":["10.1155/2016/2980297"],"title":["First Imported Case of Chikungunya Virus Infection in a Travelling Canadian Returning from the Caribbean."],"authorString":["Therrien C, Jourdan G, Holloway K, Tremblay C, Drebot MA."],"authorList":[{"author":[{"fullName":["Therrien C"],"firstName":["Christian"],"lastName":["Therrien"],"initials":["C"],"affiliation":["Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, 20045 chemin Sainte-Marie, Sainte-Anne-de-Bellevue, QC, Canada H9X 3R5."]},{"fullName":["Jourdan G"],"firstName":["Guillaume"],"lastName":["Jourdan"],"initials":["G"],"affiliation":["Hôpital de Chicoutimi, Centre de Santé et de Services Sociaux de Chicoutimi, Chicoutimi, QC, Canada."]},{"fullName":["Holloway K"],"firstName":["Kimberly"],"lastName":["Holloway"],"initials":["K"],"affiliation":["National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada."]},{"fullName":["Tremblay C"],"firstName":["Cécile"],"lastName":["Tremblay"],"initials":["C"],"affiliation":["Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, 20045 chemin Sainte-Marie, Sainte-Anne-de-Bellevue, QC, Canada H9X 3R5."]},{"fullName":["Drebot MA"],"firstName":["Michael A"],"lastName":["Drebot"],"initials":["MA"],"affiliation":["National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada."]}]}],"journalInfo":[{"volume":["2016"],"journalIssueId":["2424519"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale"],"ISOAbbreviation":["Can J Infect Dis Med Microbiol"],"medlineAbbreviation":["Can J Infect Dis Med Microbiol"],"NLMid":["101226876"],"ISSN":["1712-9532"],"ESSN":["1918-1493"]}]}],"pubYear":["2016"],"pageInfo":["2980297"],"abstractText":["This is the first Canadian case of Chikungunya virus (CHIKV) infection reported in a traveller returning from the Caribbean. Following multiple mosquito bites in Martinique Island in January 2014, the patient presented with high fever, headaches, arthralgia on both hands and feet, and a rash on the trunk upon his return to Canada. Initial serological testing for dengue virus infection was negative. Support therapy with nonsteroidal anti-inflammatory drugs was administered. The symptoms gradually improved 4 weeks after onset with residual arthralgia and morning joint stiffness. This clinical feature prompted the clinician to request CHIKV virus serology which was found to be positive for the presence of IgM and neutralizing antibodies. In 2014, over four hundred confirmed CHIKV infection cases were diagnosed in Canadian travellers returning from the Caribbean and Central America. Clinical suspicion of CHIKV or dengue virus infections should be considered in febrile patients with arthralgia returning from the recently CHIKV endemic countries of the Americas."],"affiliation":["Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, 20045 chemin Sainte-Marie, Sainte-Anne-de-Bellevue, QC, Canada H9X 3R5."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4904578?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4904578"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2016/2980297"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-01"],"dateOfCreation":["2016-07-01"],"electronicPublicationDate":["2016-02-24"],"firstPublicationDate":["2016-02-24"],"cprojectID":"PMC4904578"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnri4_8VqgcuJwvix","_score":1,"_source":{"id":["27022155"],"source":["MED"],"pmid":["27022155"],"pmcid":["PMC4889758"],"doi":["10.4269/ajtmh.16-0111"],"title":["Characterization of a Novel Murine Model to Study Zika Virus."],"authorString":["Rossi SL, Tesh RB, Azar SR, Muruato AE, Hanley KA, Auguste AJ, Langsjoen RM, Paessler S, Vasilakis N, Weaver SC."],"authorList":[{"author":[{"fullName":["Rossi SL"],"firstName":["Shannan L"],"lastName":["Rossi"],"initials":["SL"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico slrossi@utmb.edu."]},{"fullName":["Tesh RB"],"firstName":["Robert B"],"lastName":["Tesh"],"initials":["RB"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Azar SR"],"firstName":["Sasha R"],"lastName":["Azar"],"initials":["SR"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Muruato AE"],"firstName":["Antonio E"],"lastName":["Muruato"],"initials":["AE"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Hanley KA"],"firstName":["Kathryn A"],"lastName":["Hanley"],"initials":["KA"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Auguste AJ"],"firstName":["Albert J"],"lastName":["Auguste"],"initials":["AJ"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Langsjoen RM"],"firstName":["Rose M"],"lastName":["Langsjoen"],"initials":["RM"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Paessler S"],"firstName":["Slobodan"],"lastName":["Paessler"],"initials":["S"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Vasilakis N"],"firstName":["Nikos"],"lastName":["Vasilakis"],"initials":["N"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico."]}]}],"journalInfo":[{"issue":["6"],"volume":["94"],"journalIssueId":["2414127"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["The American journal of tropical medicine and hygiene"],"ISOAbbreviation":["Am. J. Trop. Med. Hyg."],"medlineAbbreviation":["Am J Trop Med Hyg"],"NLMid":["0370507"],"ISSN":["0002-9637"],"ESSN":["1476-1645"]}]}],"pubYear":["2016"],"pageInfo":["1362-1369"],"abstractText":["The mosquito-borne Zika virus (ZIKV) is responsible for an explosive ongoing outbreak of febrile illness across the Americas. ZIKV was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with Guillain-Barré syndrome and microcephaly in neonates has been detected. A previous study showed that ZIKV requires murine adaptation to generate reproducible murine disease. In our study, a low-passage Cambodian isolate caused disease and mortality in mice lacking the interferon (IFN) alpha receptor (A129 mice) in an age-dependent manner, but not in similarly aged immunocompetent mice. In A129 mice, viremia peaked at ∼10(7) plaque-forming units/mL by day 2 postinfection (PI) and reached high titers in the spleen by day 1. ZIKV was detected in the brain on day 3 PI and caused signs of neurologic disease, including tremors, by day 6. Robust replication was also noted in the testis. In this model, all mice infected at the youngest age (3 weeks) succumbed to illness by day 7 PI. Older mice (11 weeks) showed signs of illness, viremia, and weight loss but recovered starting on day 8. In addition, AG129 mice, which lack both type I and II IFN responses, supported similar infection kinetics to A129 mice, but with exaggerated disease signs. This characterization of an Asian lineage ZIKV strain in a murine model, and one of the few studies reporting a model of Zika disease and demonstrating age-dependent morbidity and mortality, could provide a platform for testing the efficacy of antivirals and vaccines."],"affiliation":["Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas; Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Institute for Translational Science, University of Texas Medical Branch, Galveston, Texas; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas; Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico slrossi@utmb.edu."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["AIM"],"name":["Core clinical journals"]},{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889758?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4889758"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4269/ajtmh.16-0111"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-02"],"dateOfRevision":["2016-06-17"],"electronicPublicationDate":["2016-03-28"],"firstPublicationDate":["2016-03-28"],"cprojectID":"PMC4889758"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrjTJ9hGurOzZIax","_score":1,"_source":{"id":["27375359"],"source":["MED"],"pmid":["27375359"],"pmcid":["PMC4918690"],"doi":["10.4137/ehi.s33384"],"title":["Effect of Trapping Methods, Weather, and Landscape on Estimates of the Culex Vector Mosquito Abundance."],"authorString":["Karki S, Hamer GL, Anderson TK, Goldberg TL, Kitron UD, Krebs BL, Walker ED, Ruiz MO."],"authorList":[{"author":[{"fullName":["Karki S"],"firstName":["Surendra"],"lastName":["Karki"],"initials":["S"],"affiliation":["Department of Pathobiology, University of Illinois, Urbana-Champaign, IL, USA."]},{"fullName":["Hamer GL"],"firstName":["Gabriel L"],"lastName":["Hamer"],"initials":["GL"],"affiliation":["Department of Entomology, Texas A&M University, College Station, TX, USA."]},{"fullName":["Anderson TK"],"firstName":["Tavis K"],"lastName":["Anderson"],"initials":["TK"],"authorId":[{"_":"0000-0002-3138-5535","$":{"type":"ORCID"}}],"affiliation":["Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, Ames, IA, USA."]},{"fullName":["Goldberg TL"],"firstName":["Tony L"],"lastName":["Goldberg"],"initials":["TL"],"affiliation":["Department of Pathobiological Sciences, University of Wisconsin, Madison, WI, USA."]},{"fullName":["Kitron UD"],"firstName":["Uriel D"],"lastName":["Kitron"],"initials":["UD"],"affiliation":["Department of Environmental Studies, Emory University, Atlanta, GA, USA."]},{"fullName":["Krebs BL"],"firstName":["Bethany L"],"lastName":["Krebs"],"initials":["BL"],"affiliation":["Wellness and Animal Behavior Department, San Francisco Zoo, San Francisco, CA, USA."]},{"fullName":["Walker ED"],"firstName":["Edward D"],"lastName":["Walker"],"initials":["ED"],"affiliation":["Department of Microbiology and Molecular Genetics, Michigan State University, Lansing, MI, USA."]},{"fullName":["Ruiz MO"],"firstName":["Marilyn O"],"lastName":["Ruiz"],"initials":["MO"],"affiliation":["Department of Pathobiology, University of Illinois, Urbana-Champaign, IL, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-3138-5535","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2400530"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Environmental health insights"],"ISOAbbreviation":["Environ Health Insights"],"medlineAbbreviation":["Environ Health Insights"],"NLMid":["101488505"],"ESSN":["1178-6302"]}]}],"pubYear":["2016"],"pageInfo":["93-103"],"abstractText":["The local abundance of Culex mosquitoes is a central factor adding to the risk of West Nile virus transmission, and vector abundance data influence public health decisions. This study evaluated differences in abundance estimates from mosquitoes trapped using two common methods: CO2-baited CDC light traps and infusion-baited gravid traps in suburban, Chicago, Illinois. On a weekly basis, the two methods were modestly correlated (r = 0.219) across 71 weeks over 4 years. Lagged weather conditions of up to four weeks were associated with the number of mosquitoes collected in light and gravid traps. Collections in light traps were higher with higher temperature in the same week, higher precipitation one, two, and four weeks before the week of trapping, and lower maximum average wind speed. Collections in gravid traps were higher with higher temperature in the same week and one week earlier, lower temperature four weeks earlier, and with higher precipitation two and four weeks earlier. Culex abundance estimates from light traps were significantly higher in semi-natural areas compared to residential areas, but abundance estimates from gravid traps did not vary by the landscape type. These results highlight the importance of the surveillance methods used in the assessment of local Culex abundance estimates. Measures of risk of exposure to West Nile virus should assess carefully how mosquito abundance has been estimated and integrated into assessments of transmission risk."],"affiliation":["Department of Pathobiology, University of Illinois, Urbana-Champaign, IL, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Culex","Abundance","Landscape","West Nile virus","Weather","Arbovirus","Light Traps","Gravid Traps"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918690?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918690"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4137/EHI.S33384"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-04"],"dateOfCreation":["2016-07-04"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-06-22"],"firstPublicationDate":["2016-06-22"],"cprojectID":"PMC4918690"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrlRJ9hGurOzZIa0","_score":1,"_source":{"id":["27350867"],"source":["MED"],"pmid":["27350867"],"pmcid":["PMC4920014"],"doi":["10.7189/jogh.06.010202"],"title":["NEWS."],"journalInfo":[{"issue":["1"],"volume":["6"],"journalIssueId":["2326444"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["Journal of global health"],"ISOAbbreviation":["J Glob Health"],"medlineAbbreviation":["J Glob Health"],"NLMid":["101578780"],"ISSN":["2047-2978"],"ESSN":["2047-2986"]}]}],"pubYear":["2016"],"pageInfo":["010202"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["News"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920014?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4920014"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.7189/jogh.06.010202"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-28"],"dateOfRevision":["2016-06-30"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4920014"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrl5hOUS2orL1IRH","_score":1,"_source":{"id":["27348752"],"source":["MED"],"pmid":["27348752"],"pmcid":["PMC4922573"],"doi":["10.1371/journal.pone.0157971"],"title":["Alarm Variables for Dengue Outbreaks: A Multi-Centre Study in Asia and Latin America."],"authorString":["Bowman LR, Tejeda GS, Coelho GE, Sulaiman LH, Gill BS, McCall PJ, Olliaro PL, Ranzinger SR, Quang LC, Ramm RS, Kroeger A, Petzold MG."],"authorList":[{"author":[{"fullName":["Bowman LR"],"firstName":["Leigh R"],"lastName":["Bowman"],"initials":["LR"],"affiliation":["UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), Geneva, Switzerland."]},{"fullName":["Tejeda GS"],"firstName":["Gustavo S"],"lastName":["Tejeda"],"initials":["GS"],"affiliation":["Ministry of Health, Mexico City, Mexico."]},{"fullName":["Coelho GE"],"firstName":["Giovanini E"],"lastName":["Coelho"],"initials":["GE"],"affiliation":["Ministry of Health, Brasilia, Brazil."]},{"fullName":["Sulaiman LH"],"firstName":["Lokman H"],"lastName":["Sulaiman"],"initials":["LH"],"affiliation":["Ministry of Health, Kuala Lumpur, Malaysia."]},{"fullName":["Gill BS"],"firstName":["Balvinder S"],"lastName":["Gill"],"initials":["BS"],"affiliation":["Ministry of Health, Kuala Lumpur, Malaysia."]},{"fullName":["McCall PJ"],"firstName":["Philip J"],"lastName":["McCall"],"initials":["PJ"],"affiliation":["Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom."]},{"fullName":["Olliaro PL"],"firstName":["Piero L"],"lastName":["Olliaro"],"initials":["PL"],"affiliation":["UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), Geneva, Switzerland."]},{"fullName":["Ranzinger SR"],"firstName":["Silvia R"],"lastName":["Ranzinger"],"initials":["SR"],"affiliation":["Institute of Public Health, University of Heidelberg, Heidelberg, Germany."]},{"fullName":["Quang LC"],"firstName":["Luong C"],"lastName":["Quang"],"initials":["LC"],"affiliation":["Pasteur Institute, Ho Chi Minh City, Vietnam."]},{"fullName":["Ramm RS"],"firstName":["Ronald S"],"lastName":["Ramm"],"initials":["RS"],"affiliation":["Ministry of Health, Santo Domingo, Dominican Republic."]},{"fullName":["Kroeger A"],"firstName":["Axel"],"lastName":["Kroeger"],"initials":["A"],"affiliation":["UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), Geneva, Switzerland."]},{"fullName":["Petzold MG"],"firstName":["Max G"],"lastName":["Petzold"],"initials":["MG"],"affiliation":["University of Gothenburg, Gothenburg, Sweden."]}]}],"journalInfo":[{"issue":["6"],"volume":["11"],"journalIssueId":["2413805"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0157971"],"abstractText":["Worldwide, dengue is an unrelenting economic and health burden. Dengue outbreaks have become increasingly common, which place great strain on health infrastructure and services. Early warning models could allow health systems and vector control programmes to respond more cost-effectively and efficiently.The Shewhart method and Endemic Channel were used to identify alarm variables that may predict dengue outbreaks. Five country datasets were compiled by epidemiological week over the years 2007-2013. These data were split between the years 2007-2011 (historic period) and 2012-2013 (evaluation period). Associations between alarm/ outbreak variables were analysed using logistic regression during the historic period while alarm and outbreak signals were captured during the evaluation period. These signals were combined to form alarm/ outbreak periods, where 2 signals were equal to 1 period. Alarm periods were quantified and used to predict subsequent outbreak periods. Across Mexico and Dominican Republic, an increase in probable cases predicted outbreaks of hospitalised cases with sensitivities and positive predictive values (PPV) of 93%/ 83% and 97%/ 86% respectively, at a lag of 1-12 weeks. An increase in mean temperature ably predicted outbreaks of hospitalised cases in Mexico and Brazil, with sensitivities and PPVs of 79%/ 73% and 81%/ 46% respectively, also at a lag of 1-12 weeks. Mean age was predictive of hospitalised cases at sensitivities and PPVs of 72%/ 74% and 96%/ 45% in Mexico and Malaysia respectively, at a lag of 4-16 weeks.An increase in probable cases was predictive of outbreaks, while meteorological variables, particularly mean temperature, demonstrated predictive potential in some countries, but not all. While it is difficult to define uniform variables applicable in every country context, the use of probable cases and meteorological variables in tailored early warning systems could be used to highlight the occurrence of dengue outbreaks or indicate increased risk of dengue transmission."],"affiliation":["UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), Geneva, Switzerland."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4922573?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4922573"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0157971"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-28"],"dateOfRevision":["2016-07-19"],"electronicPublicationDate":["2016-06-27"],"firstPublicationDate":["2016-06-27"],"cprojectID":"PMC4922573"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnron_8VqgcuJwvjA","_score":1,"_source":{"id":["27294787"],"source":["MED"],"pmid":["27294787"],"pmcid":["PMC4905668"],"doi":["10.1371/journal.pntd.0004795"],"title":["Digital Participatory Surveillance and the Zika Crisis: Opportunities and Caveats."],"authorString":["Pagliari C, Vijaykumar S."],"authorList":[{"author":[{"fullName":["Pagliari C"],"firstName":["Claudia"],"lastName":["Pagliari"],"initials":["C"],"authorId":[{"_":"0000-0001-5791-7723","$":{"type":"ORCID"}}],"affiliation":["Edinburgh Global Health Academy, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh Medical School, Edinburgh, United Kingdom."]},{"fullName":["Vijaykumar S"],"firstName":["Santosh"],"lastName":["Vijaykumar"],"initials":["S"],"affiliation":["Emerging Technology Lab, School of Computer Science and Engineering, Nanyang Technological University, Singapore."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-5791-7723","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004795"],"affiliation":["Edinburgh Global Health Academy, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh Medical School, Edinburgh, United Kingdom."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4905668?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4905668"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004795"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-14"],"dateOfRevision":["2016-06-29"],"electronicPublicationDate":["2016-06-13"],"firstPublicationDate":["2016-06-13"],"cprojectID":"PMC4905668"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnroq_8VqgcuJwvjE","_score":1,"_source":{"id":["27332714"],"source":["MED"],"pmid":["27332714"],"pmcid":["PMC4917180"],"doi":["10.1371/journal.pntd.0004817"],"title":["ZIKV - CDB: A Collaborative Database to Guide Research Linking SncRNAs and ZIKA Virus Disease Symptoms."],"authorString":["Pylro VS, Oliveira FS, Morais DK, Cuadros-Orellana S, Pais FS, Medeiros JD, Geraldo JA, Gilbert J, Volpini AC, Fernandes GR."],"authorList":[{"author":[{"fullName":["Pylro VS"],"firstName":["Victor Satler"],"lastName":["Pylro"],"initials":["VS"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Oliveira FS"],"firstName":["Francislon Silva"],"lastName":["Oliveira"],"initials":["FS"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Morais DK"],"firstName":["Daniel Kumazawa"],"lastName":["Morais"],"initials":["DK"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Cuadros-Orellana S"],"firstName":["Sara"],"lastName":["Cuadros-Orellana"],"initials":["S"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Pais FS"],"firstName":["Fabiano Sviatopolk-Mirsky"],"lastName":["Pais"],"initials":["FS"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Medeiros JD"],"firstName":["Julliane Dutra"],"lastName":["Medeiros"],"initials":["JD"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Geraldo JA"],"firstName":["Juliana Assis"],"lastName":["Geraldo"],"initials":["JA"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Gilbert J"],"firstName":["Jack"],"lastName":["Gilbert"],"initials":["J"],"affiliation":["Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America."]},{"fullName":["Volpini AC"],"firstName":["Angela Cristina"],"lastName":["Volpini"],"initials":["AC"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]},{"fullName":["Fernandes GR"],"firstName":["Gabriel Rocha"],"lastName":["Fernandes"],"initials":["GR"],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."]}]}],"journalInfo":[{"issue":["6"],"volume":["10"],"journalIssueId":["2413877"],"dateOfPublication":["2016 Jun"],"monthOfPublication":["6"],"yearOfPublication":["2016"],"printPublicationDate":["2016-06-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004817"],"abstractText":["In early 2015, a ZIKA Virus (ZIKV) infection outbreak was recognized in northeast Brazil, where concerns over its possible links with infant microcephaly have been discussed. Providing a causal link between ZIKV infection and birth defects is still a challenge. MicroRNAs (miRNAs) are small noncoding RNAs (sncRNAs) that regulate post-transcriptional gene expression by translational repression, and play important roles in viral pathogenesis and brain development. The potential for flavivirus-mediated miRNA signalling dysfunction in brain-tissue development provides a compelling hypothesis to test the perceived link between ZIKV and microcephaly.Here, we applied in silico analyses to provide novel insights to understand how Congenital ZIKA Syndrome symptoms may be related to an imbalance in miRNAs function. Moreover, following World Health Organization (WHO) recommendations, we have assembled a database to help target investigations of the possible relationship between ZIKV symptoms and miRNA-mediated human gene expression.We have computationally predicted both miRNAs encoded by ZIKV able to target genes in the human genome and cellular (human) miRNAs capable of interacting with ZIKV genomes. Our results represent a step forward in the ZIKV studies, providing new insights to support research in this field and identify potential targets for therapy."],"affiliation":["Biosystems Informatics and Genomics Group, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917180?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4917180"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004817"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["refseq"," gen"," "]}],"dateOfCreation":["2016-06-23"],"dateOfRevision":["2016-07-09"],"electronicPublicationDate":["2016-06-22"],"firstPublicationDate":["2016-06-22"],"cprojectID":"PMC4917180"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrorhOUS2orL1IRP","_score":1,"_source":{"id":["27403405"],"source":["MED"],"pmid":["27403405"],"pmcid":["PMC4928130"],"doi":["10.1089/biores.2016.0004"],"title":["Pathogenesis of Zika Virus-Associated Embryopathy."],"authorString":["Mawson AR."],"authorList":[{"author":[{"fullName":["Mawson AR"],"firstName":["Anthony R"],"lastName":["Mawson"],"initials":["AR"],"affiliation":["Department of Epidemiology and Biostatistics, School of Public Health (Initiative), Jackson State University , Jackson, Mississippi."]}]}],"journalInfo":[{"issue":["1"],"volume":["5"],"journalIssueId":["2373829"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BioResearch open access"],"ISOAbbreviation":["Biores Open Access"],"medlineAbbreviation":["Biores Open Access"],"NLMid":["101579333"],"ISSN":["2164-7844"],"ESSN":["2164-7860"]}]}],"pubYear":["2016"],"pageInfo":["171-176"],"abstractText":["A strong causal association has become evident between Zika virus (ZIKV) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. Circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the Guillain-Barré syndrome, are due to an endogenous form of hypervitaminosis A resulting from ZIKV infection-induced damage to the liver and the spillage of stored vitamin A compounds (\"retinoids\") into the maternal and fetal circulation in toxic concentrations. Retinoids are mainly stored in the liver (about 80%) and are essential for numerous biological functions. In higher concentration, retinoids are potentially cytotoxic, pro-oxidant, mutagenic and teratogenic, especially if sudden shifts occur in their bodily distribution. Although liver involvement has not been mentioned specifically in recent reports, conventional liver enzyme tests underestimate the true extent of liver dysfunction. The proposed model could be tested by comparing retinoid concentration and expression profiles in microcephalic newborns of ZIKV-infected mothers and nonmicrocephalic newborn controls, and by correlating these profiles with measures of clinical severity."],"affiliation":["Department of Epidemiology and Biostatistics, School of Public Health (Initiative), Jackson State University , Jackson, Mississippi."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Liver","Pregnancy","Virus","Retinoids","Microcephaly","Guillain-barré Syndrome","Hypervitaminosis A","Embryopathy","Zika"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928130?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4928130"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1089/biores.2016.0004"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-12"],"dateOfCreation":["2016-07-12"],"dateOfRevision":["2016-07-14"],"electronicPublicationDate":["2016-06-01"],"firstPublicationDate":["2016-06-01"],"cprojectID":"PMC4928130"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnronJ9hGurOzZIa-","_score":1,"_source":{"id":["27419140"],"source":["MED"],"pmid":["27419140"],"pmcid":["PMC4932163"],"doi":["10.1155/2016/8603263"],"title":["Resistance Status to the Insecticides Temephos, Deltamethrin, and Diflubenzuron in Brazilian Aedes aegypti Populations."],"authorString":["Bellinato DF, Viana-Medeiros PF, Araújo SC, Martins AJ, Lima JB, Valle D."],"authorList":[{"author":[{"fullName":["Bellinato DF"],"firstName":["Diogo Fernandes"],"lastName":["Bellinato"],"initials":["DF"],"authorId":[{"_":"0000-0002-6768-0085","$":{"type":"ORCID"}}],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil."]},{"fullName":["Viana-Medeiros PF"],"firstName":["Priscila Fernandes"],"lastName":["Viana-Medeiros"],"initials":["PF"],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil."]},{"fullName":["Araújo SC"],"firstName":["Simone Costa"],"lastName":["Araújo"],"initials":["SC"],"affiliation":["Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil; Gerência de Controle de Zoonoses, Secretaria Municipal de Saúde, Belo Horizonte, MG, Brazil."]},{"fullName":["Martins AJ"],"firstName":["Ademir J"],"lastName":["Martins"],"initials":["AJ"],"affiliation":["Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil."]},{"fullName":["Lima JB"],"firstName":["José Bento Pereira"],"lastName":["Lima"],"initials":["JB"],"affiliation":["Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil."]},{"fullName":["Valle D"],"firstName":["Denise"],"lastName":["Valle"],"initials":["D"],"authorId":[{"_":"0000-0003-4148-274X","$":{"type":"ORCID"}}],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6768-0085","$":{"type":"ORCID"}},{"_":"0000-0003-4148-274X","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["2016"],"journalIssueId":["2375656"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BioMed research international"],"ISOAbbreviation":["Biomed Res Int"],"medlineAbbreviation":["Biomed Res Int"],"NLMid":["101600173"],"ISSN":["2314-6133"],"ESSN":["2314-6141"]}]}],"pubYear":["2016"],"pageInfo":["8603263"],"abstractText":["Insecticides are still largely applied in public health to control disease vectors. In Brazil, organophosphates (OP) and pyrethroids (PY) are used against Aedes aegypti for years. Since 2009 Insect Growth Regulators (IGR) are also employed in the control of larvae. We quantified resistance to temephos (OP), deltamethrin (PY), and diflubenzuron (IGR) of A. aegypti samples from 12 municipalities distributed throughout the country, collected between 2010 and 2012. High levels of resistance to neurotoxic insecticides were detected in almost all populations: RR95 to temephos varied between 4.0 and 27.1; the lowest RR95 to deltamethrin was 13.1, and values higher than 70.0 were found. In contrast, all samples were susceptible to diflubenzuron (RR95 < 2.3). Biochemical tests performed with larvae and adults discarded the participation of acetylcholinesterase, the OP target, and confirmed involvement of the detoxifying enzymes esterases, mixed function oxidases, and glutathione-S-transferases. The results obtained were discussed taking into account the public chemical control component and the increase in the domestic use of insecticides during dengue epidemic seasons in the evaluated municipalities."],"affiliation":["Laboratório de Biologia Molecular de Flavivírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4932163?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4932163"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2016/8603263"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-15"],"dateOfRevision":["2016-07-17"],"electronicPublicationDate":["2016-06-21"],"firstPublicationDate":["2016-06-21"],"cprojectID":"PMC4932163"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrpc_8VqgcuJwvjH","_score":1,"_source":{"id":["27070380"],"source":["MED"],"pmid":["27070380"],"pmcid":["PMC4918175"],"doi":["10.3201/eid2207.151990"],"title":["A Literature Review of Zika Virus."],"authorString":["Plourde AR, Bloch EM."],"authorList":[{"author":[{"fullName":["Plourde AR"],"firstName":["Anna R"],"lastName":["Plourde"],"initials":["AR"]},{"fullName":["Bloch EM"],"firstName":["Evan M"],"lastName":["Bloch"],"initials":["EM"]}]}],"journalInfo":[{"issue":["7"],"volume":["22"],"journalIssueId":["2397213"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1185-1192"],"abstractText":["Zika virus is a mosquitoborne flavivirus that is the focus of an ongoing pandemic and public health emergency. Previously limited to sporadic cases in Africa and Asia, the emergence of Zika virus in Brazil in 2015 heralded rapid spread throughout the Americas. Although most Zika virus infections are characterized by subclinical or mild influenza-like illness, severe manifestations have been described, including Guillain-Barre syndrome in adults and microcephaly in babies born to infected mothers. Neither an effective treatment nor a vaccine is available for Zika virus; therefore, the public health response primarily focuses on preventing infection, particularly in pregnant women. Despite growing knowledge about this virus, questions remain regarding the virus's vectors and reservoirs, pathogenesis, genetic diversity, and potential synergistic effects of co-infection with other circulating viruses. These questions highlight the need for research to optimize surveillance, patient management, and public health intervention in the current Zika virus epidemic."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Viruses","Aedes","Mosquitoes","Zoonoses","Microcephaly","Arbovirus","Emerging Infectious Diseases","Flavivirus","Review Literature As Topic","Craniofacial Abnormalities","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918175?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4918175"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2207.151990"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-06-17"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-07-15"],"firstPublicationDate":["2016-07-15"],"embargoDate":["2016-07-01"],"cprojectID":"PMC4918175"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq9N_8VqgcuJwvg9","_score":1,"_source":{"id":["26527535"],"source":["MED"],"pmid":["26527535"],"pmcid":["PMC4629289"],"doi":["10.1186/s12879-015-1231-2"],"title":["Potential of selected Senegalese Aedes spp. mosquitoes (Diptera: Culicidae) to transmit Zika virus."],"authorString":["Diagne CT, Diallo D, Faye O, Ba Y, Faye O, Gaye A, Dia I, Faye O, Weaver SC, Sall AA, Diallo M."],"authorList":[{"author":[{"fullName":["Diagne CT"],"firstName":["Cheikh Tidiane"],"lastName":["Diagne"],"initials":["CT"],"affiliation":["Département de Biologie Animale, Laboratoire d'Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal. ctdiagne@pasteur.sn."]},{"fullName":["Diallo D"],"firstName":["Diawo"],"lastName":["Diallo"],"initials":["D"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. ddiallo@pasteur.sn."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Unité des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. oumarfaye@pasteur.sn."]},{"fullName":["Ba Y"],"firstName":["Yamar"],"lastName":["Ba"],"initials":["Y"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. ba@pasteur.sn."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Unité des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. ofaye@pasteur.sn."]},{"fullName":["Gaye A"],"firstName":["Alioune"],"lastName":["Gaye"],"initials":["A"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. agaye@pasteur.sn."]},{"fullName":["Dia I"],"firstName":["Ibrahima"],"lastName":["Dia"],"initials":["I"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. dia@pasteur.sn."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Département de Biologie Animale, Laboratoire d'Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal. fayeo@orange.sn."]},{"fullName":["Weaver SC"],"firstName":["Scott C"],"lastName":["Weaver"],"initials":["SC"],"affiliation":["Department of Pathology and Microbiology & Immunology, University of Texas Medical Branch, Galveston, TX, 77555-0610, USA. sweaver@utmb.edu."]},{"fullName":["Sall AA"],"firstName":["Amadou Alpha"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Unité des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. asall@pasteur.sn."]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"],"affiliation":["Unité d'Entomologie Médicale, Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220,, Dakar, Senegal. diallo@pasteur.sn."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2262623"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["BMC infectious diseases"],"ISOAbbreviation":["BMC Infect. Dis."],"medlineAbbreviation":["BMC Infect Dis"],"NLMid":["100968551"],"ESSN":["1471-2334"]}]}],"pubYear":["2015"],"pageInfo":["492"],"abstractText":["Zika virus (ZIKV; genus Flavivirus, family Flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal Aedes spp. mosquitoes and nonhuman primates in African and Asian forests. Serological evidence and virus isolations have demonstrated widespread distribution of the virus in Senegal. Several mosquito species have been found naturally infected by ZIKV but little is known about their vector competence.We assessed the vector competence of Ae. aegypti from Kedougou and Dakar, Ae. unilineatus, Ae. vittatus and Ae. luteocephalus from Kedougou in Senegal for 6 ZIKV strains using experimental oral infection. Fully engorged female mosquitoes were maintained in an environmental chamber set at 27 ± 1 °C and 80 ± 5% Relative humidity. At day 5, 10 and 15 days post infection (dpi), individual mosquito saliva, legs/wings and bodies were tested for the presence of ZIKV genome using real time RT-PCR to estimate the infection, dissemination, and transmission rates.All the species tested were infected by all viral strains but only Ae. vittatus and Ae. luteocephalus were potentially capable of transmitting ZIKV after 15 dpi with 20 and 50% of mosquitoes, respectively, delivering epidemic (HD 78788) and prototype (MR 766) ZIKV strains in saliva.All the species tested here were susceptible to oral infection of ZIKV but only a low proportion of Ae. vittatus and Ae. luteocephalus had the viral genome in their saliva and thus the potential to transmit the virus. Further investigations are needed on the vector competence of other species associated with ZIKV for better understanding of the ecology and epidemiology of this virus in Senegal."],"affiliation":["Département de Biologie Animale, Laboratoire d'Écologie Vectorielle et Parasitaire, Université Cheikh Anta Diop, Dakar, Senegal. ctdiagne@pasteur.sn."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R01AI069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Saliva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Senegal"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Real-Time Polymerase Chain Reaction"]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4629289?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4629289"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12879-015-1231-2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["4"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-22"],"dateOfCreation":["2015-11-03"],"dateOfRevision":["2015-11-05"],"electronicPublicationDate":["2015-11-02"],"firstPublicationDate":["2015-11-02"],"cprojectID":"PMC4629289"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-WJ9hGurOzZIZD","_score":1,"_source":{"id":["26969623"],"source":["MED"],"pmid":["26969623"],"pmcid":["PMC4788873"],"doi":["10.1186/s12936-016-1208-7"],"title":["Genetic diversity of Plasmodium falciparum isolates from concurrent malaria and arbovirus co-infections in Kedougou, southeastern Senegal."],"authorString":["Niang M, Loucoubar C, Sow A, Diagne MM, Faye O, Faye O, Diallo M, Toure-Balde A, Sall AA."],"authorList":[{"author":[{"fullName":["Niang M"],"firstName":["Makhtar"],"lastName":["Niang"],"initials":["M"],"affiliation":["Immunology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal. mniang@pasteur.sn."]},{"fullName":["Loucoubar C"],"firstName":["Cheikh"],"lastName":["Loucoubar"],"initials":["C"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Sow A"],"firstName":["Abdourahmane"],"lastName":["Sow"],"initials":["A"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Diagne MM"],"firstName":["Moussa Moise"],"lastName":["Diagne"],"initials":["MM"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Faye O"],"firstName":["Oumar"],"lastName":["Faye"],"initials":["O"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Faye O"],"firstName":["Ousmane"],"lastName":["Faye"],"initials":["O"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Diallo M"],"firstName":["Mawlouth"],"lastName":["Diallo"],"initials":["M"],"affiliation":["Medical Entomology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Toure-Balde A"],"firstName":["Aissatou"],"lastName":["Toure-Balde"],"initials":["A"],"affiliation":["Immunology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]},{"fullName":["Sall AA"],"firstName":["Amadou A"],"lastName":["Sall"],"initials":["AA"],"affiliation":["Arbovirus and Viral Hemorrhagic Fevers Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal."]}]}],"journalInfo":[{"volume":["15"],"journalIssueId":["2401987"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Malaria journal"],"ISOAbbreviation":["Malar. J."],"medlineAbbreviation":["Malar J"],"NLMid":["101139802"],"ESSN":["1475-2875"]}]}],"pubYear":["2016"],"pageInfo":["155"],"abstractText":["Concurrent malaria and arbovirus infections are common and represent an important public health concern in regions where both diseases are endemic. The present study investigates the genetic diversity and complexity of Plasmodium falciparum infection in concurrent malaria-arbovirus infections in Kedougou region, southeastern Senegal.Parasite DNA was extracted from 60 to 27 sera samples collected from P. falciparum isolates of malaria and concurrent malaria-arbovirus infected patients, respectively, and followed by PCR-genotyping targeting the msp-1 (block2) and msp-2 (block3) allelic families.The mean number of genotype per allelic family was comparable between the two groups. K1 was the predominant msp-1 allelic type both in malaria (94.91%) and arbovirus-malaria (92.59%) groups, whereas IC/3D7 was the most prevalent msp-2 allelic type in malaria (94.91%) and arbovirus-malaria (96.29%) groups. Frequencies of msp-1 and msp-2 allelic types were statistically comparable between the two groups (Fisher exact test, P > 0.05) and were not associated with age. FC27 was strikingly the least prevalent in both groups and was absent in children under 5 years of age. The proportions of P. falciparum isolates from malaria-infected patients carrying the three msp-1 allelic types (67.44%) or the two msp-2 allelic types (76.47%) were significantly higher than those from arbovirus-malaria co-infected patients (Exact binomial test, P < 0.05). The multiplicities of infection (MOI) were low and comparable for msp-1 (1.19 vs 1.22) and msp-2 (1.11 vs 1.10), respectively between malaria and arbovirus-malaria groups.The study showed no difference in the genetic diversity between P. falciparum isolates from malaria and concurrent malaria-arbovirus infected patients in Kedougou. The MOI was low despite intense malaria transmission in Kedougou. The overall results suggest a limited or no influence of arbovirus infections on P. falciparum diversity and complexity of malaria infection."],"affiliation":["Immunology Unit, Institut Pasteur Dakar, 36 Avenue Pasteur, 220, Dakar, Senegal. mniang@pasteur.sn."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["AI1069145"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"keywordList":[{"keyword":["Malaria","Genetic diversity","Multiplicity of infection","Arbovirus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4788873?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4788873"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12936-016-1208-7"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-12"],"dateOfRevision":["2016-03-14"],"electronicPublicationDate":["2016-03-11"],"firstPublicationDate":["2016-03-11"],"cprojectID":"PMC4788873"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq-t_8VqgcuJwvhC","_score":1,"_source":{"id":["26645066"],"source":["MED"],"pmid":["26645066"],"pmcid":["PMC4673751"],"doi":["10.1186/s12985-015-0444-8"],"title":["Tackling dengue fever: Current status and challenges."],"authorString":["Nedjadi T, El-Kafrawy S, Sohrab SS, Desprès P, Damanhouri G, Azhar E."],"authorList":[{"author":[{"fullName":["Nedjadi T"],"firstName":["Taoufik"],"lastName":["Nedjadi"],"initials":["T"],"affiliation":["King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. tnedjadi@kau.edu.sa."]},{"fullName":["El-Kafrawy S"],"firstName":["Sherif"],"lastName":["El-Kafrawy"],"initials":["S"],"affiliation":["Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. saelkfrawy@kau.edu.sa."]},{"fullName":["Sohrab SS"],"firstName":["Sayed S"],"lastName":["Sohrab"],"initials":["SS"],"affiliation":["Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. ssohrab@kau.edu.sa."]},{"fullName":["Desprès P"],"firstName":["Philippe"],"lastName":["Desprès"],"initials":["P"],"affiliation":["UMR PIMIT (I2T team), University of Reunion island, INSERM U1187, CNRS 9192, IRD 249, Technology Platform CYROI, 2 rue Maxime Rivière Saint-Clotilde, La Reunion, 97491, France. philippe.despres@univ-reunion.fr."]},{"fullName":["Damanhouri G"],"firstName":["Ghazi"],"lastName":["Damanhouri"],"initials":["G"],"affiliation":["King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. gdamanhouri@kau.edu.sa."]},{"fullName":["Azhar E"],"firstName":["Esam"],"lastName":["Azhar"],"initials":["E"],"affiliation":["Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. eazhar@kau.edu.sa."]}]}],"journalInfo":[{"volume":["12"],"journalIssueId":["2267380"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2015"],"pageInfo":["212"],"abstractText":["According to recent statistics, 96 million apparent dengue infections were estimated worldwide in 2010. This figure is by far greater than the WHO prediction which indicates the rapid spread of this disease posing a growing threat to the economy and a major challenge to clinicians and health care services across the globe particularly in the affected areas.This article aims at bringing to light the current epidemiological and clinical status of the dengue fever. The relationship between genetic mutations, single nucleotide polymorphism (SNP) and the pathophysiology of disease progression will be put into perspective. It will also highlight the recent advances in dengue vaccine development.Thus far, a significant progress has been made in unraveling the risk factors and understanding the molecular pathogenesis associated with the disease. However, further insights in molecular features of the disease and the development of animal models will enormously help improving the therapeutic interventions and potentially contribute to finding new preventive measures for population at risk."],"affiliation":["King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. tnedjadi@kau.edu.sa."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Dengue"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Disease Models, Animal"]},{"majorTopic_YN":["N"],"descriptorName":["Communicable Disease Control"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["MT"],"qualifierName":["methods"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Dengue Vaccines"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Dengue Vaccines"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4673751?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4673751"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-015-0444-8"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-06-29"],"dateOfCreation":["2015-12-09"],"dateOfRevision":["2015-12-14"],"electronicPublicationDate":["2015-12-09"],"firstPublicationDate":["2015-12-09"],"cprojectID":"PMC4673751"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrHfhOUS2orL1IPi","_score":1,"_source":{"id":["27001187"],"source":["MED"],"pmid":["27001187"],"pmcid":["PMC4802632"],"doi":["10.1186/s12916-016-0601-y"],"title":["Zika virus and neurologic autoimmunity: the putative role of gangliosides."],"authorString":["Anaya JM, Ramirez-Santana C, Salgado-Castaneda I, Chang C, Ansari A, Gershwin ME."],"authorList":[{"author":[{"fullName":["Anaya JM"],"firstName":["Juan-Manuel"],"lastName":["Anaya"],"initials":["JM"],"authorId":[{"_":"0000-0002-6444-1249","$":{"type":"ORCID"}}],"affiliation":["Center for Autoimmune Diseases Research (CREA), Universidad del Rosario, Bogota, Colombia. anayajm@gmail.com."]},{"fullName":["Ramirez-Santana C"],"firstName":["Carolina"],"lastName":["Ramirez-Santana"],"initials":["C"],"affiliation":["Center for Autoimmune Diseases Research (CREA), Universidad del Rosario, Bogota, Colombia."]},{"fullName":["Salgado-Castaneda I"],"firstName":["Ignacio"],"lastName":["Salgado-Castaneda"],"initials":["I"],"affiliation":["Servicio de Neurología, Hospital Central de la Policía, Bogota, Colombia."]},{"fullName":["Chang C"],"firstName":["Christopher"],"lastName":["Chang"],"initials":["C"],"affiliation":["Division of Rheumatology, Allergy and Clinical Immunology, University of California Davis, School of Medicine, Davis, CA, USA."]},{"fullName":["Ansari A"],"firstName":["Aftab"],"lastName":["Ansari"],"initials":["A"],"affiliation":["Department of Pathology, Emory University School of Medicine, Atlanta, GA, USA."]},{"fullName":["Gershwin ME"],"firstName":["M Eric"],"lastName":["Gershwin"],"initials":["ME"],"affiliation":["Division of Rheumatology, Allergy and Clinical Immunology, University of California Davis, School of Medicine, Davis, CA, USA."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-6444-1249","$":{"type":"ORCID"}},{"_":"0000-0002-6444-1249","$":{"type":"ORCID"}},{"_":"0000-0003-2137-4899","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["14"],"journalIssueId":["2401375"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC medicine"],"ISOAbbreviation":["BMC Med"],"medlineAbbreviation":["BMC Med"],"NLMid":["101190723"],"ESSN":["1741-7015"]}]}],"pubYear":["2016"],"pageInfo":["49"],"abstractText":["An increasing number of severe neurological complications associated with Zika virus (ZIKV), chiefly Guillain-Barré syndrome (GBS) and primary microcephaly, have led the World Health Organization to declare a global health emergency. Molecular mimicry between glycolipids and surface molecules of infectious agents explain most of the cases of GBS preceded by infection, while a direct toxicity of ZIKV on neural cells has been raised as the main mechanism by which ZIKV induces microcephaly. Gangliosides are crucial in brain development, and their expression correlates with neurogenesis, synaptogenesis, synaptic transmission, and cell proliferation. Targeting the autoimmune response to gangliosides may represent an underexploited opportunity to examine the increased incidence of neurological complications related to ZIKV infection."],"affiliation":["Center for Autoimmune Diseases Research (CREA), Universidad del Rosario, Bogota, Colombia. anayajm@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Brain"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Microcephaly"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Guillain-Barre Syndrome"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Gangliosides"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Autoimmunity"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Mimicry"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["IM"],"qualifierName":["immunology"],"majorTopic_YN":["Y"]}]}]}]}],"keywordList":[{"keyword":["Gangliosides","Autoimmunity","Microcephaly","Guillain-barré Syndrome","Zika Virus"]}],"chemicalList":[{"chemical":[{"name":["Gangliosides"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4802632?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4802632"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12916-016-0601-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-15"],"dateOfCreation":["2016-03-22"],"dateOfRevision":["2016-03-25"],"electronicPublicationDate":["2016-03-21"],"firstPublicationDate":["2016-03-21"],"cprojectID":"PMC4802632"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrGiJ9hGurOzZIZV","_score":1,"_source":{"id":["26980239"],"source":["MED"],"pmid":["26980239"],"pmcid":["PMC4820678"],"doi":["10.1038/emi.2016.48"],"title":["Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic."],"authorString":["Zhu Z, Chan JF, Tee KM, Choi GK, Lau SK, Woo PC, Tse H, Yuen KY."],"authorList":[{"author":[{"fullName":["Zhu Z"],"firstName":["Zheng"],"lastName":["Zhu"],"initials":["Z"],"affiliation":["Department of Microbiology, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Chan JF"],"firstName":["Jasper Fuk-Woo"],"lastName":["Chan"],"initials":["JF"],"affiliation":["Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Tee KM"],"firstName":["Kah-Meng"],"lastName":["Tee"],"initials":["KM"],"affiliation":["Department of Microbiology, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Choi GK"],"firstName":["Garnet Kwan-Yue"],"lastName":["Choi"],"initials":["GK"],"affiliation":["Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Lau SK"],"firstName":["Susanna Kar-Pui"],"lastName":["Lau"],"initials":["SK"],"affiliation":["Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Woo PC"],"firstName":["Patrick Chiu-Yat"],"lastName":["Woo"],"initials":["PC"],"affiliation":["Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Tse H"],"firstName":["Herman"],"lastName":["Tse"],"initials":["H"],"affiliation":["Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China."]},{"fullName":["Yuen KY"],"firstName":["Kwok-Yung"],"lastName":["Yuen"],"initials":["KY"],"affiliation":["Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2370343"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Emerging microbes & infections"],"ISOAbbreviation":["Emerg Microbes Infect"],"medlineAbbreviation":["Emerg Microbes Infect"],"NLMid":["101594885"],"ESSN":["2222-1751"]}]}],"pubYear":["2016"],"pageInfo":["e22"],"abstractText":["Less than 20 sporadic cases of human Zika virus (ZIKV) infection were reported in Africa and Asia before 2007, but large outbreaks involving up to 73% of the populations on the Pacific islands have started since 2007, and spread to the Americas in 2014. Moreover, the clinical manifestation of ZIKV infection has apparently changed, as evident by increasing reports of neurological complications, such as Guillain-Barré syndrome in adults and congenital anomalies in neonates. We comprehensively compared the genome sequences of pre-epidemic and epidemic ZIKV strains with complete genome or complete polyprotein sequences available in GenBank. Besides the reported phylogenetic clustering of the epidemic strains with the Asian lineage, we found that the topology of phylogenetic tree of all coding regions is the same except that of the non-structural 2B (NS2B) coding region. This finding was confirmed by bootscan analysis and multiple sequence alignment, which suggested the presence of a fragment of genetic recombination at NS2B with that of Spondweni virus. Moreover, the representative epidemic strain possesses one large bulge of nine bases instead of an external loop on the first stem-loop structure at the 3'-untranslated region just distal to the stop codon of the NS5 in the 1947 pre-epidemic prototype strain. Fifteen amino acid substitutions are found in the epidemic strains when compared with the pre-epidemic strains. As mutations in other flaviviruses can be associated with changes in virulence, replication efficiency, antigenic epitopes and host tropism, further studies would be important to ascertain the biological significance of these genomic changes."],"affiliation":["Department of Microbiology, The University of Hong Kong, Hong Kong, China."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4820678?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4820678"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/emi.2016.48"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-03-16"],"dateOfRevision":["2016-04-18"],"electronicPublicationDate":["2016-03-16"],"firstPublicationDate":["2016-03-16"],"cprojectID":"PMC4820678"}}
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It was isolated in São Paulo state, Brazil, in 2015, from a patient who received a blood transfusion from an asymptomatic donor at the time of donation."],"affiliation":["Division of Vector-Borne Diseases, Adolfo Lutz Institute, São Paulo, Brazil."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777745?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4777745"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00032-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-03-04"],"dateOfCreation":["2016-03-04"],"dateOfRevision":["2016-03-23"],"electronicPublicationDate":["2016-03-03"],"firstPublicationDate":["2016-03-03"],"cprojectID":"PMC4777745"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnr6rJ9hGurOzZIcF","_score":1,"_source":{"id":["27144515"],"source":["MED"],"pmid":["27144515"],"pmcid":["PMC4982160"],"doi":["10.3201/eid2208.160496"],"title":["Time Lags between Exanthematous Illness Attributed to Zika Virus, Guillain-Barre Syndrome, and Microcephaly, Salvador, Brazil."],"authorString":["Paploski IA, Prates AP, Cardoso CW, Kikuti M, Silva MM, Waller LA, Reis MG, Kitron U, Ribeiro GS."],"authorList":[{"author":[{"fullName":["Paploski IA"],"firstName":["Igor A D"],"lastName":["Paploski"],"initials":["IA"]},{"fullName":["Prates AP"],"firstName":["Ana Paula P B"],"lastName":["Prates"],"initials":["AP"]},{"fullName":["Cardoso CW"],"firstName":["Cristiane W"],"lastName":["Cardoso"],"initials":["CW"]},{"fullName":["Kikuti M"],"firstName":["Mariana"],"lastName":["Kikuti"],"initials":["M"]},{"fullName":["Silva MM"],"firstName":["Monaise M O"],"lastName":["Silva"],"initials":["MM"]},{"fullName":["Waller LA"],"firstName":["Lance A"],"lastName":["Waller"],"initials":["LA"]},{"fullName":["Reis MG"],"firstName":["Mitermayer G"],"lastName":["Reis"],"initials":["MG"]},{"fullName":["Kitron U"],"firstName":["Uriel"],"lastName":["Kitron"],"initials":["U"]},{"fullName":["Ribeiro GS"],"firstName":["Guilherme S"],"lastName":["Ribeiro"],"initials":["GS"]}]}],"journalInfo":[{"issue":["8"],"volume":["22"],"journalIssueId":["2403977"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["1438-1444"],"abstractText":["Zika virus infection emerged as a public health emergency after increasing evidence for its association with neurologic disorders and congenital malformations. In Salvador, Brazil, outbreaks of acute exanthematous illness (AEI) attributed to Zika virus, Guillain-Barré syndrome (GBS), and microcephaly occurred in 2015. We investigated temporal correlations and time lags between these outbreaks to identify a common link between them by using epidemic curves and time series cross-correlations. Number of GBS cases peaked after a lag of 5-9 weeks from the AEI peak. Number of suspected cases of microcephaly peaked after a lag of 30-33 weeks from the AEI peak, which corresponded to time of potential infections of pregnant mothers during the first trimester. These findings support the association of GBS and microcephaly with Zika virus infection and provide evidence for a temporal relationship between timing of arboviral infection of pregnant women during the first trimester and birth outcome."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Viruses","Brazil","Pregnant women","epidemiology","Outbreaks","Microcephaly","Guillain-barré Syndrome","Time Lags","Salvador","Zika Virus","Exanthematous Illness"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982160?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4982160"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2208.160496"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-20"],"dateOfRevision":["2016-08-14"],"electronicPublicationDate":["2016-08-15"],"firstPublicationDate":["2016-08-15"],"cprojectID":"PMC4982160"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr9QhOUS2orL1ISc","_score":1,"_source":{"id":["27556644"],"source":["MED"],"pmid":["27556644"],"pmcid":["PMC4996428"],"doi":["10.1371/journal.pntd.0004921"],"title":["Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements."],"authorString":["Viktorovskaya OV, Greco TM, Cristea IM, Thompson SR."],"authorList":[{"author":[{"fullName":["Viktorovskaya OV"],"firstName":["Olga V"],"lastName":["Viktorovskaya"],"initials":["OV"],"affiliation":["Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America."]},{"fullName":["Greco TM"],"firstName":["Todd M"],"lastName":["Greco"],"initials":["TM"],"affiliation":["Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America."]},{"fullName":["Cristea IM"],"firstName":["Ileana M"],"lastName":["Cristea"],"initials":["IM"],"affiliation":["Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America."]},{"fullName":["Thompson SR"],"firstName":["Sunnie R"],"lastName":["Thompson"],"initials":["SR"],"affiliation":["Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America."]}]}],"journalInfo":[{"issue":["8"],"volume":["10"],"journalIssueId":["2435547"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004921"],"abstractText":["There are currently no vaccines or antivirals available for dengue virus infection, which can cause dengue hemorrhagic fever and death. A better understanding of the host pathogen interaction is required to develop effective therapies to treat DENV. In particular, very little is known about how cellular RNA binding proteins interact with viral RNAs. RNAs within cells are not naked; rather they are coated with proteins that affect localization, stability, translation and (for viruses) replication.Seventy-nine novel RNA binding proteins for dengue virus (DENV) were identified by cross-linking proteins to dengue viral RNA during a live infection in human cells. These cellular proteins were specific and distinct from those previously identified for poliovirus, suggesting a specialized role for these factors in DENV amplification. Knockdown of these proteins demonstrated their function as viral host factors, with evidence for some factors acting early, while others late in infection. Their requirement by DENV for efficient amplification is likely specific, since protein knockdown did not impair the cell fitness for viral amplification of an unrelated virus. The protein abundances of these host factors were not significantly altered during DENV infection, suggesting their interaction with DENV RNA was due to specific recruitment mechanisms. However, at the global proteome level, DENV altered the abundances of proteins in particular classes, including transporter proteins, which were down regulated, and proteins in the ubiquitin proteasome pathway, which were up regulated.The method for identification of host factors described here is robust and broadly applicable to all RNA viruses, providing an avenue to determine the conserved or distinct mechanisms through which diverse viruses manage the viral RNA within cells. This study significantly increases the number of cellular factors known to interact with DENV and reveals how DENV modulates and usurps cellular proteins for efficient amplification."],"affiliation":["Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996428?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996428"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004921"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-25"],"electronicPublicationDate":["2016-08-24"],"firstPublicationDate":["2016-08-24"],"cprojectID":"PMC4996428"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr_P_8VqgcuJwvkP","_score":1,"_source":{"id":["27491989"],"source":["MED"],"pmid":["27491989"],"pmcid":["PMC4974310"],"doi":["10.1128/genomea.00750-16"],"title":["Complete Genome Sequence of Zika Virus Isolated in Mexico, 2016."],"authorString":["Díaz-Quiñonez JA, Peña-Alonso R, Mendieta-Condado E, Garcés-Ayala F, González-Durán E, Escobar-Escamilla N, Vázquez-Pichardo M, Torres-Rodríguez Mde L, Núñez-León A, Torres-Longoria B, López-Martínez I, Ruíz-Matus C, Kuri-Morales P, Ramírez-González JE."],"authorList":[{"author":[{"fullName":["Díaz-Quiñonez JA"],"firstName":["José Alberto"],"lastName":["Díaz-Quiñonez"],"initials":["JA"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico Facultad de Medicina, UNAM, Mexico City, Mexico alberto.diaz@salud.gob.mx ernesto.ramirez@salud.gob.mx."]},{"fullName":["Peña-Alonso R"],"firstName":["Rocío"],"lastName":["Peña-Alonso"],"initials":["R"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Mendieta-Condado E"],"firstName":["Edgar"],"lastName":["Mendieta-Condado"],"initials":["E"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Garcés-Ayala F"],"firstName":["Fabiola"],"lastName":["Garcés-Ayala"],"initials":["F"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["González-Durán E"],"firstName":["Elizabeth"],"lastName":["González-Durán"],"initials":["E"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Escobar-Escamilla N"],"firstName":["Noé"],"lastName":["Escobar-Escamilla"],"initials":["N"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico Escuela Nacional de Ciencias Biológicas, IPN, Mexico City, Mexico."]},{"fullName":["Vázquez-Pichardo M"],"firstName":["Mauricio"],"lastName":["Vázquez-Pichardo"],"initials":["M"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Torres-Rodríguez Mde L"],"firstName":["María de la Luz"],"lastName":["Torres-Rodríguez"],"initials":["Mde L"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Núñez-León A"],"firstName":["Alma"],"lastName":["Núñez-León"],"initials":["A"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Torres-Longoria B"],"firstName":["Belem"],"lastName":["Torres-Longoria"],"initials":["B"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["López-Martínez I"],"firstName":["Irma"],"lastName":["López-Martínez"],"initials":["I"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico."]},{"fullName":["Ruíz-Matus C"],"firstName":["Cuitláhuac"],"lastName":["Ruíz-Matus"],"initials":["C"],"affiliation":["Dirección General de Epidemiología, Mexico City, Mexico."]},{"fullName":["Kuri-Morales P"],"firstName":["Pablo"],"lastName":["Kuri-Morales"],"initials":["P"],"affiliation":["Subsecretaría de Prevención y Promoción de la Salud, Mexico City, Mexico."]},{"fullName":["Ramírez-González JE"],"firstName":["José Ernesto"],"lastName":["Ramírez-González"],"initials":["JE"],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico alberto.diaz@salud.gob.mx ernesto.ramirez@salud.gob.mx."]}]}],"journalInfo":[{"issue":["4"],"volume":["4"],"journalIssueId":["2427904"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome announcements"],"ISOAbbreviation":["Genome Announc"],"medlineAbbreviation":["Genome Announc"],"NLMid":["101595808"],"ESSN":["2169-8287"]}]}],"pubYear":["2016"],"abstractText":["Zika virus belongs to the genus Flavivirus, and its spread remains an international public health emergency. In this report, we describe the obtainment and molecular characterization of a complete viral genome through the direct metagenomic analysis from saliva from an autochthonous transmission case in Mexico."],"affiliation":["Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) Dr. Manuel Martínez Báez, Mexico City, Mexico Facultad de Medicina, UNAM, Mexico City, Mexico alberto.diaz@salud.gob.mx ernesto.ramirez@salud.gob.mx."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974310?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4974310"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/genomeA.00750-16"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2016-08-05"],"dateOfCreation":["2016-08-05"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-08-04"],"firstPublicationDate":["2016-08-04"],"cprojectID":"PMC4974310"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr_YhOUS2orL1ISf","_score":1,"_source":{"id":["27562436"],"source":["MED"],"pmid":["27562436"],"pmcid":["PMC4997733"],"doi":["10.1186/s13073-016-0344-6"],"title":["Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens."],"authorString":["Doan T, Wilson MR, Crawford ED, Chow ED, Khan LM, Knopp KA, O'Donovan BD, Xia D, Hacker JK, Stewart JM, Gonzales JA, Acharya NR, DeRisi JL."],"authorList":[{"author":[{"fullName":["Doan T"],"firstName":["Thuy"],"lastName":["Doan"],"initials":["T"],"affiliation":["Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Wilson MR"],"firstName":["Michael R"],"lastName":["Wilson"],"initials":["MR"],"affiliation":["Department of Neurology, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Crawford ED"],"firstName":["Emily D"],"lastName":["Crawford"],"initials":["ED"],"affiliation":["Howard Hughes Medical Institute, Chevy Chase, MD, USA."]},{"fullName":["Chow ED"],"firstName":["Eric D"],"lastName":["Chow"],"initials":["ED"],"affiliation":["Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Khan LM"],"firstName":["Lillian M"],"lastName":["Khan"],"initials":["LM"],"affiliation":["Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Knopp KA"],"firstName":["Kristeene A"],"lastName":["Knopp"],"initials":["KA"],"affiliation":["Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["O'Donovan BD"],"firstName":["Brian D"],"lastName":["O'Donovan"],"initials":["BD"],"affiliation":["Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Xia D"],"firstName":["Dongxiang"],"lastName":["Xia"],"initials":["D"],"affiliation":["California Department of Public Health, Richmond, CA, USA."]},{"fullName":["Hacker JK"],"firstName":["Jill K"],"lastName":["Hacker"],"initials":["JK"],"affiliation":["California Department of Public Health, Richmond, CA, USA."]},{"fullName":["Stewart JM"],"firstName":["Jay M"],"lastName":["Stewart"],"initials":["JM"],"affiliation":["Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Gonzales JA"],"firstName":["John A"],"lastName":["Gonzales"],"initials":["JA"],"affiliation":["Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["Acharya NR"],"firstName":["Nisha R"],"lastName":["Acharya"],"initials":["NR"],"affiliation":["Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USA."]},{"fullName":["DeRisi JL"],"firstName":["Joseph L"],"lastName":["DeRisi"],"initials":["JL"],"affiliation":["Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA. joe@derisilab.ucsf.edu."]}]}],"journalInfo":[{"issue":["1"],"volume":["8"],"journalIssueId":["2359926"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome medicine"],"ISOAbbreviation":["Genome Med"],"medlineAbbreviation":["Genome Med"],"NLMid":["101475844"],"ESSN":["1756-994X"]}]}],"pubYear":["2016"],"pageInfo":["90"],"abstractText":["Ocular infections remain a major cause of blindness and morbidity worldwide. While prognosis is dependent on the timing and accuracy of diagnosis, the etiology remains elusive in ~50 % of presumed infectious uveitis cases. The objective of this study is to determine if unbiased metagenomic deep sequencing (MDS) can accurately detect pathogens in intraocular fluid samples of patients with uveitis.This is a proof-of-concept study, in which intraocular fluid samples were obtained from five subjects with known diagnoses, and one subject with bilateral chronic uveitis without a known etiology. Samples were subjected to MDS, and results were compared with those from conventional diagnostic tests. Pathogens were identified using a rapid computational pipeline to analyze the non-host sequences obtained from MDS.Unbiased MDS of intraocular fluid produced results concordant with known diagnoses in subjects with (n = 4) and without (n = 1) uveitis. Samples positive for Cryptococcus neoformans, Toxoplasma gondii, and herpes simplex virus 1 as tested by a Clinical Laboratory Improvement Amendments-certified laboratory were correctly identified with MDS. Rubella virus was identified in one case of chronic bilateral idiopathic uveitis. The subject's strain was most closely related to a German rubella virus strain isolated in 1992, one year before he developed a fever and rash while living in Germany. The pattern and the number of viral identified mutations present in the patient's strain were consistent with long-term viral replication in the eye.MDS can identify fungi, parasites, and DNA and RNA viruses in minute volumes of intraocular fluid samples. The identification of chronic intraocular rubella virus infection highlights the eye's role as a long-term pathogen reservoir, which has implications for virus eradication and emerging global epidemics."],"affiliation":["Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Rubella virus","Uveitis","Pathogen Discovery","Metagenomic Deep Sequencing"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4997733?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4997733"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13073-016-0344-6"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-08-26"],"dateOfRevision":["2016-08-28"],"electronicPublicationDate":["2016-08-25"],"firstPublicationDate":["2016-08-25"],"cprojectID":"PMC4997733"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsAX_8VqgcuJwvkT","_score":1,"_source":{"id":["27501302"],"source":["MED"],"pmid":["27501302"],"pmcid":["PMC4976904"],"doi":["10.1371/journal.pone.0160230"],"title":["The Critical Role of Early Dengue Surveillance and Limitations of Clinical Reporting - Implications for Non-Endemic Countries."],"authorString":["Kao JH, Chen CD, Tiger Li ZR, Chan TC, Tung TH, Chu YH, Cheng HY, Liu JW, Shih FY, Shu PY, Lin CC, Tsai WH, Ku CC, Ho CK, King CC."],"authorList":[{"author":[{"fullName":["Kao JH"],"firstName":["Jui-Hung"],"lastName":["Kao"],"initials":["JH"],"affiliation":["Department of Medicine (Med.), College of Med., National Taiwan University (NTU), Taipei, Taiwan (100), Republic of China (R.O.C.)."]},{"fullName":["Chen CD"],"firstName":["Chaur-Dong"],"lastName":["Chen"],"initials":["CD"],"affiliation":["Department of Health, Kaohsiung City Government, Kaohsiung, Taiwan (802), R.O.C."]},{"fullName":["Tiger Li ZR"],"firstName":["Zheng-Rong"],"lastName":["Tiger Li"],"initials":["ZR"],"affiliation":["Institute of Epidemiology and Preventive Med., College of Public Health, NTU, Taipei, Taiwan (100), R.O.C."]},{"fullName":["Chan TC"],"firstName":["Ta-Chien"],"lastName":["Chan"],"initials":["TC"],"affiliation":["Research Center for Humanities & Social Science, Academia Sinica, Taipei, Taiwan (115), R.O.C."]},{"fullName":["Tung TH"],"firstName":["Tsung-Hwa"],"lastName":["Tung"],"initials":["TH"],"affiliation":["Institute of Epidemiology and Preventive Med., College of Public Health, NTU, Taipei, Taiwan (100), R.O.C."]},{"fullName":["Chu YH"],"firstName":["Yin-Hsia"],"lastName":["Chu"],"initials":["YH"],"affiliation":["Department of Medicine (Med.), College of Med., National Taiwan University (NTU), Taipei, Taiwan (100), Republic of China (R.O.C.)."]},{"fullName":["Cheng HY"],"firstName":["Hau-Yuan"],"lastName":["Cheng"],"initials":["HY"],"affiliation":["Department of Pediatrics, NTU Hospital, Taipei, Taiwan (100), R.O.C."]},{"fullName":["Liu JW"],"firstName":["Jien-Wei"],"lastName":["Liu"],"initials":["JW"],"affiliation":["Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University Medical College, Kaohsiung, Taiwan (833), R.O.C."]},{"fullName":["Shih FY"],"firstName":["Fuh-Yuan"],"lastName":["Shih"],"initials":["FY"],"affiliation":["Department of Emergency Medicine, NTU Hospital, Taipei, Taiwan (100), R.O.C."]},{"fullName":["Shu PY"],"firstName":["Pei-Yun"],"lastName":["Shu"],"initials":["PY"],"affiliation":["Department of Health, Taiwan Centers for Disease Control (Taiwan CDC), Taipei, Taiwan (100), R.O.C."]},{"fullName":["Lin CC"],"firstName":["Chien-Chou"],"lastName":["Lin"],"initials":["CC"],"affiliation":["Department of Health, Taiwan Centers for Disease Control (Taiwan CDC), Taipei, Taiwan (100), R.O.C."]},{"fullName":["Tsai WH"],"firstName":["Wu-Hsiung"],"lastName":["Tsai"],"initials":["WH"],"affiliation":["Department of Health, Kaohsiung City Government, Kaohsiung, Taiwan (802), R.O.C."]},{"fullName":["Ku CC"],"firstName":["Chia-Chi"],"lastName":["Ku"],"initials":["CC"],"affiliation":["Institute of Immunology, College of Medicine, NTU, Taipei, Taiwan (100), R.O.C."]},{"fullName":["Ho CK"],"firstName":["Chi-Kung"],"lastName":["Ho"],"initials":["CK"],"affiliation":["Department of Public Health, Kaohsiung Medical University, and Dept. of Occupational and Environmental Med., Kaohsiung Medical University Hospital, Kaohsiung, Taiwan (807), R.O.C."]},{"fullName":["King CC"],"firstName":["Chwan-Chuen"],"lastName":["King"],"initials":["CC"],"affiliation":["Institute of Epidemiology and Preventive Med., College of Public Health, NTU, Taipei, Taiwan (100), R.O.C."]}]}],"journalInfo":[{"issue":["8"],"volume":["11"],"journalIssueId":["2435494"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0160230"],"abstractText":["The increasing dengue burden and epidemic severity worldwide have highlighted the need to improve surveillance. In non-endemic areas such as Taiwan, where outbreaks start mostly with imported cases from Southeast Asia, a closer examination of surveillance dynamics to detect cases early is necessary. To evaluate problems with dengue surveillance and investigate the involvement of different factors at various epidemic stages, we investigated 632 laboratory-confirmed indigenous dengue cases in Kaohsiung City, Taiwan during 2009-2010. The estimated sensitivity of clinical surveillance was 82.4% (521/632). Initially, the modified serological surveillance (targeting only the contacts of laboratory-confirmed dengue cases) identified clinically unrecognized afebrile cases in younger patients who visited private clinics and accounted for 30.4% (35/115) of the early-stage cases. Multivariate regression indicated that hospital/medical center visits [Adjusted Odds Ratio (aOR): 11.6, 95% confidence interval (CI): 6.3-21.4], middle epidemic stage [aOR: 2.4 (1.2-4.7)], fever [aOR: 2.3 (2.3-12.9)], and musculo-articular pain [aOR: 1.9 (1.05-3.3)] were significantly associated with clinical reporting. However, cases with pruritus/rash [aOR: 0.47 (0.26-0.83)] and diarrhea [aOR: 0.47 (0.26-0.85)] were underreported. In conclusion, multiple factors contributed to dengue surveillance problems. To prevent a large-scale epidemic and minimize severe dengue cases, there is a need for integrated surveillance incorporating entomological, clinical, serological, and virological surveillance systems to detect early cases, followed by immediate prevention and control measures and continuous evaluation to ensure effectiveness. This effort will be particularly important for an arbovirus, such as Zika virus, with a high asymptomatic infection ratio. For dengue- non-endemic countries, we recommend serological surveillance be implemented in areas with high Aedes mosquito indices or many breeding sites. Syndromic surveillance, spatial analysis and monitoring changes in epidemiological characteristics using a geographical information system, as well as epidemic prediction models involving epidemiological, meteorological and environmental variables will be helpful for early risk communication to increase awareness."],"affiliation":["Department of Medicine (Med.), College of Med., National Taiwan University (NTU), Taipei, Taiwan (100), Republic of China (R.O.C.)."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976904?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4976904"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0160230"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-09"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-08"],"firstPublicationDate":["2016-08-08"],"cprojectID":"PMC4976904"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsBx_8VqgcuJwvka","_score":1,"_source":{"id":["27430951"],"source":["MED"],"pmid":["27430951"],"pmcid":["PMC4980333"],"doi":["10.1007/s13238-016-0293-2"],"title":["Structural basis of Zika virus helicase in recognizing its substrates."],"authorString":["Tian H, Ji X, Yang X, Zhang Z, Lu Z, Yang K, Chen C, Zhao Q, Chi H, Mu Z, Xie W, Wang Z, Lou H, Yang H, Rao Z."],"authorList":[{"author":[{"fullName":["Tian H"],"firstName":["Hongliang"],"lastName":["Tian"],"initials":["H"],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China."]},{"fullName":["Ji X"],"firstName":["Xiaoyun"],"lastName":["Ji"],"initials":["X"],"affiliation":["The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China."]},{"fullName":["Yang X"],"firstName":["Xiaoyun"],"lastName":["Yang"],"initials":["X"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Zhang Z"],"firstName":["Zhongxin"],"lastName":["Zhang"],"initials":["Z"],"affiliation":["State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China."]},{"fullName":["Lu Z"],"firstName":["Zuokun"],"lastName":["Lu"],"initials":["Z"],"affiliation":["College of Life Sciences, Nankai University, Tianjin, 300071, China."]},{"fullName":["Yang K"],"firstName":["Kailin"],"lastName":["Yang"],"initials":["K"],"affiliation":["Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, 44195, USA."]},{"fullName":["Chen C"],"firstName":["Cheng"],"lastName":["Chen"],"initials":["C"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Zhao Q"],"firstName":["Qi"],"lastName":["Zhao"],"initials":["Q"],"affiliation":["Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA."]},{"fullName":["Chi H"],"firstName":["Heng"],"lastName":["Chi"],"initials":["H"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Mu Z"],"firstName":["Zhongyu"],"lastName":["Mu"],"initials":["Z"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Xie W"],"firstName":["Wei"],"lastName":["Xie"],"initials":["W"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Wang Z"],"firstName":["Zefang"],"lastName":["Wang"],"initials":["Z"],"affiliation":["School of Life Sciences, Tianjin University, Tianjin, 300072, China."]},{"fullName":["Lou H"],"firstName":["Huiqiang"],"lastName":["Lou"],"initials":["H"],"affiliation":["State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China."]},{"fullName":["Yang H"],"firstName":["Haitao"],"lastName":["Yang"],"initials":["H"],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China. yanght@tju.edu.cn."]},{"fullName":["Rao Z"],"firstName":["Zihe"],"lastName":["Rao"],"initials":["Z"],"affiliation":["College of Life Sciences, Nankai University, Tianjin, 300071, China."]}]}],"journalInfo":[{"issue":["8"],"volume":["7"],"journalIssueId":["2440452"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Protein & cell"],"ISOAbbreviation":["Protein Cell"],"medlineAbbreviation":["Protein Cell"],"NLMid":["101532368"],"ISSN":["1674-800X"],"ESSN":["1674-8018"]}]}],"pubYear":["2016"],"pageInfo":["562-570"],"abstractText":["The recent explosive outbreak of Zika virus (ZIKV) infection has been reported in South and Central America and the Caribbean. Neonatal microcephaly associated with ZIKV infection has already caused a public health emergency of international concern. No specific vaccines or drugs are currently available to treat ZIKV infection. The ZIKV helicase, which plays a pivotal role in viral RNA replication, is an attractive target for therapy. We determined the crystal structures of ZIKV helicase-ATP-Mn(2+) and ZIKV helicase-RNA. This is the first structure of any flavivirus helicase bound to ATP. Comparisons with related flavivirus helicases have shown that although the critical P-loop in the active site has variable conformations among different species, it adopts an identical mode to recognize ATP/Mn(2+). The structure of ZIKV helicase-RNA has revealed that upon RNA binding, rotations of the motor domains can cause significant conformational changes. Strikingly, although ZIKV and dengue virus (DENV) apo-helicases share conserved residues for RNA binding, their different manners of motor domain rotations result in distinct individual modes for RNA recognition. It suggests that flavivirus helicases could have evolved a conserved engine to convert chemical energy from nucleoside triphosphate to mechanical energy for RNA unwinding, but different motor domain rotations result in variable RNA recognition modes to adapt to individual viral replication."],"affiliation":["Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["ATP","Crystal structure","Flavivirus","Helicase","Zika Virus"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980333?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4980333"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1007/s13238-016-0293-2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["PDB"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["pdb"]}],"dateOfCreation":["2016-08-11"],"dateOfRevision":["2016-08-23"],"electronicPublicationDate":["2016-07-18"],"firstPublicationDate":["2016-07-18"],"cprojectID":"PMC4980333"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDVJ9hGurOzZIcV","_score":1,"_source":{"id":["27558513"],"source":["MED"],"pmid":["27558513"],"pmcid":["PMC4996801"],"doi":["10.1186/s40169-016-0107-4"],"title":["A One Health overview, facilitating advances in comparative medicine and translational research."],"authorString":["Stroud C, Dmitriev I, Kashentseva E, Bryan JN, Curiel DT, Rindt H, Reinero C, Henry CJ, Bergman PJ, Mason NJ, Gnanandarajah JS, Engiles JB, Gray F, Laughlin D, Gaurnier-Hausser A, Wallecha A, Huebner M, Paterson Y, O'Connor D, Treml LS, Stannard JP, Cook JL, Jacobs M, Wyckoff GJ, Likins L, Sabbagh U, Skaff A, Guloy AS, Hays HD, LeBlanc AK, Coates JR, Katz ML, Lyons LA, Johnson GC, Johnson GS, O'Brien DP, Duan D, Calvet JP, Gandolfi B, Baron DA, Weiss ML, Webster DA, Karanu FN, Robb EJ, Harman RJ."],"authorList":[{"author":[{"fullName":["Stroud C"],"firstName":["Cheryl"],"lastName":["Stroud"],"initials":["C"],"affiliation":["One Health Commission, Apex, NC, 27502, USA. cstroud@onehealthcommission.org."]},{"fullName":["Dmitriev I"],"firstName":["Igor"],"lastName":["Dmitriev"],"initials":["I"],"affiliation":["Biologic Therapeutics Center, Washington University in St. Louis, St. Louis, MO, 63130, USA."]},{"fullName":["Kashentseva E"],"firstName":["Elena"],"lastName":["Kashentseva"],"initials":["E"],"affiliation":["Biologic Therapeutics Center, Washington University in St. Louis, St. Louis, MO, 63130, USA."]},{"fullName":["Bryan JN"],"firstName":["Jeffrey N"],"lastName":["Bryan"],"initials":["JN"],"affiliation":["Comparative Oncology, Radiobiology, and Epigenetics Laboratory, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Curiel DT"],"firstName":["David T"],"lastName":["Curiel"],"initials":["DT"],"affiliation":["Biologic Therapeutics Center, Washington University in St. Louis, St. Louis, MO, 63130, USA."]},{"fullName":["Rindt H"],"firstName":["Hans"],"lastName":["Rindt"],"initials":["H"],"affiliation":["Comparative Internal Medicine Laboratory, University of Missouri, Columbia, MO, 65203, USA."]},{"fullName":["Reinero C"],"firstName":["Carol"],"lastName":["Reinero"],"initials":["C"],"affiliation":["Comparative Internal Medicine Laboratory, University of Missouri, Columbia, MO, 65203, USA."]},{"fullName":["Henry CJ"],"firstName":["Carolyn J"],"lastName":["Henry"],"initials":["CJ"],"affiliation":["Comparative Oncology, Radiobiology, and Epigenetics Laboratory, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Bergman PJ"],"firstName":["Philip J"],"lastName":["Bergman"],"initials":["PJ"],"affiliation":["Adjunct Associate Faculty, Memorial Sloan-Kettering Cancer Center, New York City, NY, 10065, USA."]},{"fullName":["Mason NJ"],"firstName":["Nicola J"],"lastName":["Mason"],"initials":["NJ"],"affiliation":["University of Pennsylvania School of Veterinary Medicine, 3900 Delancey Street, Philadelphia, PA, 19104, USA."]},{"fullName":["Gnanandarajah JS"],"firstName":["Josephine S"],"lastName":["Gnanandarajah"],"initials":["JS"],"affiliation":["University of Pennsylvania School of Veterinary Medicine, 3900 Delancey Street, Philadelphia, PA, 19104, USA."]},{"fullName":["Engiles JB"],"firstName":["Julie B"],"lastName":["Engiles"],"initials":["JB"],"affiliation":["University of Pennsylvania School of Veterinary Medicine, 3900 Delancey Street, Philadelphia, PA, 19104, USA."]},{"fullName":["Gray F"],"firstName":["Falon"],"lastName":["Gray"],"initials":["F"],"affiliation":["University of Pennsylvania School of Veterinary Medicine, 3900 Delancey Street, Philadelphia, PA, 19104, USA."]},{"fullName":["Laughlin D"],"firstName":["Danielle"],"lastName":["Laughlin"],"initials":["D"],"affiliation":["University of Pennsylvania School of Veterinary Medicine, 3900 Delancey Street, Philadelphia, PA, 19104, USA."]},{"fullName":["Gaurnier-Hausser A"],"firstName":["Anita"],"lastName":["Gaurnier-Hausser"],"initials":["A"],"affiliation":["Office of Professional Studies in the Health Sciences, Drexel University College of Medicine, Room 4801 New College Building, 245 North 15th Street, Philadelphia, PA, 19102, USA."]},{"fullName":["Wallecha A"],"firstName":["Anu"],"lastName":["Wallecha"],"initials":["A"],"affiliation":["Advaxis Immunotherapies Inc., 305 College Road East, Princeton, NJ, 08540, USA."]},{"fullName":["Huebner M"],"firstName":["Margie"],"lastName":["Huebner"],"initials":["M"],"affiliation":["ClinData Services Inc., 6713 Holyoke Court, Fort Collins, CO, 80525, USA."]},{"fullName":["Paterson Y"],"firstName":["Yvonne"],"lastName":["Paterson"],"initials":["Y"],"affiliation":["Department of Microbiology, University of Pennsylvania Perelman School of Medicine, 319A Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA, 19104, USA."]},{"fullName":["O'Connor D"],"firstName":["Daniel"],"lastName":["O'Connor"],"initials":["D"],"affiliation":["Advaxis Immunotherapies Inc., 305 College Road East, Princeton, NJ, 08540, USA."]},{"fullName":["Treml LS"],"firstName":["Laura S"],"lastName":["Treml"],"initials":["LS"],"affiliation":["Aratana Therapeutics Inc., Leawood, KS, 66211, USA."]},{"fullName":["Stannard JP"],"firstName":["James P"],"lastName":["Stannard"],"initials":["JP"],"affiliation":["Department of Orthopaedic Surgery, Missouri Orthopaedic Institute, 1100 Virginia Ave., Columbia, MO, 65212, USA. stannardj@health.missouri.edu."]},{"fullName":["Cook JL"],"firstName":["James L"],"lastName":["Cook"],"initials":["JL"],"affiliation":["Comparative Orthopaedic Lab, Mizzou BioJoint Center, Missouri Orthopaedic Institute, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Jacobs M"],"firstName":["Marc"],"lastName":["Jacobs"],"initials":["M"],"affiliation":["Musculoskeletal Transplant Foundation (MTF), Edison, NJ, 08837, USA."]},{"fullName":["Wyckoff GJ"],"firstName":["Gerald J"],"lastName":["Wyckoff"],"initials":["GJ"],"affiliation":["Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, 64110, USA."]},{"fullName":["Likins L"],"firstName":["Lee"],"lastName":["Likins"],"initials":["L"],"affiliation":["Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, 64110, USA."]},{"fullName":["Sabbagh U"],"firstName":["Ubadah"],"lastName":["Sabbagh"],"initials":["U"],"affiliation":["Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, 64110, USA."]},{"fullName":["Skaff A"],"firstName":["Andrew"],"lastName":["Skaff"],"initials":["A"],"affiliation":["Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO, 64110, USA."]},{"fullName":["Guloy AS"],"firstName":["Amado S"],"lastName":["Guloy"],"initials":["AS"],"affiliation":["Rex Animal Health, Kansas City, KS, 66103, USA. amado@rexanimalhealth.com."]},{"fullName":["Hays HD"],"firstName":["Harlen D"],"lastName":["Hays"],"initials":["HD"],"affiliation":["Cerner Corporation, Kansas City, MO, 64117, USA."]},{"fullName":["LeBlanc AK"],"firstName":["Amy K"],"lastName":["LeBlanc"],"initials":["AK"],"affiliation":["Comparative Oncology Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA."]},{"fullName":["Coates JR"],"firstName":["Joan R"],"lastName":["Coates"],"initials":["JR"],"affiliation":["Departments of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO, 65211, USA. CoatesJ@missouri.edu."]},{"fullName":["Katz ML"],"firstName":["Martin L"],"lastName":["Katz"],"initials":["ML"],"affiliation":["Mason Eye Institute, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Lyons LA"],"firstName":["Leslie A"],"lastName":["Lyons"],"initials":["LA"],"affiliation":["Departments of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO, 65211, USA. lyonsla@missouri.edu."]},{"fullName":["Johnson GC"],"firstName":["Gayle C"],"lastName":["Johnson"],"initials":["GC"],"affiliation":["Veterinary Pathobiology, Comparative Neurology Program, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Johnson GS"],"firstName":["Gary S"],"lastName":["Johnson"],"initials":["GS"],"affiliation":["Veterinary Pathobiology, Comparative Neurology Program, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["O'Brien DP"],"firstName":["Dennis P"],"lastName":["O'Brien"],"initials":["DP"],"affiliation":["Departments of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Duan D"],"firstName":["Dongsheng"],"lastName":["Duan"],"initials":["D"],"affiliation":["Department of Molecular Microbiology and Immunology, Department of Neurology, Department of Bioengineering, University of Missouri, Columbia, MO, 65212, USA."]},{"fullName":["Calvet JP"],"firstName":["James P"],"lastName":["Calvet"],"initials":["JP"],"affiliation":["Kidney Institute, University of Kansas Medical Center, Kansas City, KS, 66160, USA."]},{"fullName":["Gandolfi B"],"firstName":["Barbara"],"lastName":["Gandolfi"],"initials":["B"],"affiliation":["Departments of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO, 65211, USA."]},{"fullName":["Baron DA"],"firstName":["David A"],"lastName":["Baron"],"initials":["DA"],"affiliation":["Polycystic Kidney Disease Foundation, Kansas City, MO, 64114, USA. davidb@pkdcure.org."]},{"fullName":["Weiss ML"],"firstName":["Mark L"],"lastName":["Weiss"],"initials":["ML"],"affiliation":["Department of Anatomy and Physiology, Kansas State University College of Veterinary Medicine, Manhattan, KS, 66506, USA."]},{"fullName":["Webster DA"],"firstName":["Debra A"],"lastName":["Webster"],"initials":["DA"],"affiliation":["Cardinal Health Regulatory Sciences, Overland Park, KS, 64078, USA. debra.webster@cardinalhealth.com."]},{"fullName":["Karanu FN"],"firstName":["Francis N"],"lastName":["Karanu"],"initials":["FN"],"affiliation":["Likarda LLC, Kansas City, KS, 66103, USA. fkaranu@likarda.com."]},{"fullName":["Robb EJ"],"firstName":["Edward J"],"lastName":["Robb"],"initials":["EJ"],"affiliation":["Likarda LLC, Kansas City, KS, 66103, USA."]},{"fullName":["Harman RJ"],"firstName":["Robert J"],"lastName":["Harman"],"initials":["RJ"],"affiliation":["VetStem Biopharma, Inc., Poway, CA, 92064, USA. bharman@vetstem.com."]}]}],"journalInfo":[{"issue":["Suppl 1"],"volume":["5"],"journalIssueId":["2443907"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["Clinical and translational medicine"],"ISOAbbreviation":["Clin Transl Med"],"medlineAbbreviation":["Clin Transl Med"],"NLMid":["101597971"],"ESSN":["2001-1326"]}]}],"pubYear":["2016"],"pageInfo":["26"],"abstractText":["A1 One health advances and successes in comparative medicine and translational researchCheryl StroudA2 Dendritic cell-targeted gorilla adenoviral vector for cancer vaccination for canine melanomaIgor Dmitriev, Elena Kashentseva, Jeffrey N. Bryan, David T. CurielA3 Viroimmunotherapy for malignant melanoma in the companion dog modelJeffrey N. Bryan, David Curiel, Igor Dmitriev, Elena Kashentseva, Hans Rindt, Carol Reinero, Carolyn J. HenryA4 Of mice and men (and dogs!): development of a commercially licensed xenogeneic DNA vaccine for companion animals with malignant melanomaPhilip J. BergmanA5 Successful immunotherapy with a recombinant HER2-expressing Listeria monocytogenes in dogs with spontaneous osteosarcoma paves the way for advances in pediatric osteosarcomaNicola J. Mason, Josephine S. Gnanandarajah, Julie B. Engiles, Falon Gray, Danielle Laughlin, Anita Gaurnier-Hausser, Anu Wallecha, Margie Huebner, Yvonne PatersonA6 Human clinical development of ADXS-HER2Daniel O'ConnorA7 Leveraging use of data for both human and veterinary benefitLaura S. TremlA8 Biologic replacement of the knee: innovations and early clinical resultsJames P. StannardA9 Mizzou BioJoint Center: a translational success storyJames L. CookA10 University and industry translational partnership: from the lab to commercializationMarc JacobsA11 Beyond docking: an evolutionarily guided OneHealth approach to drug discoveryGerald J. Wyckoff, Lee Likins, Ubadah Sabbagh, Andrew SkaffA12 Challenges and opportunities for data applications in animal health: from precision medicine to precision husbandryAmado S. GuloyA13 A cloud-based programmable platform for healthHarlen D. HaysA14 Comparative oncology: One Health in actionAmy K. LeBlancA15 Companion animal diseases bridge the translational gap for human neurodegenerative diseaseJoan R. Coates, Martin L. Katz, Leslie A. Lyons, Gayle C. Johnson, Gary S. Johnson, Dennis P. O'BrienA16 Duchenne muscular dystrophy gene therapyDongsheng DuanA17 Polycystic kidney disease: cellular mechanisms to emerging therapiesJames P. CalvetA18 The domestic cat as a large animal model for polycystic kidney diseaseLeslie A. Lyons, Barbara GandolfiA19 The support of basic and clinical research by the Polycystic Kidney Disease FoundationDavid A. BaronA20 Using naturally occurring large animal models of human disease to enable clinical translation: treatment of arthritis using autologous stromal vascular fraction in dogsMark L. WeissA21 Regulatory requirements regarding clinical use of human cells, tissues, and tissue-based productsDebra A. WebsterA22 Regenerative medicine approaches to Type 1 diabetes treatmentFrancis N. KaranuA23 The zoobiquity of canine diabetes mellitus, man's best friend is a friend indeed-islet transplantationEdward J. RobbA24 One Medicine: a development model for cellular therapy of diabetesRobert J. Harman."],"affiliation":["One Health Commission, Apex, NC, 27502, USA. cstroud@onehealthcommission.org."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996801?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4996801"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40169-016-0107-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-25"],"dateOfCreation":["2016-08-25"],"dateOfRevision":["2016-09-09"],"firstPublicationDate":["2016-08-01"],"cprojectID":"PMC4996801"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDdJ9hGurOzZIcW","_score":1,"_source":{"id":["27453325"],"source":["MED"],"pmid":["27453325"],"pmcid":["PMC5006693"],"doi":["10.1016/j.ebiom.2016.07.018"],"title":["Comparative Analysis Between Flaviviruses Reveals Specific Neural Stem Cell Tropism for Zika Virus in the Mouse Developing Neocortex."],"authorString":["Brault JB, Khou C, Basset J, Coquand L, Fraisier V, Frenkiel MP, Goud B, Manuguerra JC, Pardigon N, Baffet AD."],"authorList":[{"author":[{"fullName":["Brault JB"],"firstName":["Jean-Baptiste"],"lastName":["Brault"],"initials":["JB"],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France."]},{"fullName":["Khou C"],"firstName":["Cécile"],"lastName":["Khou"],"initials":["C"],"affiliation":["Institut Pasteur, ERI/CIBU Arbovirus Group, 25 rue du Dr Roux, 75015 Paris, France."]},{"fullName":["Basset J"],"firstName":["Justine"],"lastName":["Basset"],"initials":["J"],"affiliation":["Institut Pasteur, ERI/CIBU Arbovirus Group, 25 rue du Dr Roux, 75015 Paris, France."]},{"fullName":["Coquand L"],"firstName":["Laure"],"lastName":["Coquand"],"initials":["L"],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France."]},{"fullName":["Fraisier V"],"firstName":["Vincent"],"lastName":["Fraisier"],"initials":["V"],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France."]},{"fullName":["Frenkiel MP"],"firstName":["Marie-Pascale"],"lastName":["Frenkiel"],"initials":["MP"],"affiliation":["Institut Pasteur, ERI/CIBU Arbovirus Group, 25 rue du Dr Roux, 75015 Paris, France."]},{"fullName":["Goud B"],"firstName":["Bruno"],"lastName":["Goud"],"initials":["B"],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France."]},{"fullName":["Manuguerra JC"],"firstName":["Jean-Claude"],"lastName":["Manuguerra"],"initials":["JC"],"affiliation":["Institut Pasteur, ERI/CIBU Arbovirus Group, 25 rue du Dr Roux, 75015 Paris, France."]},{"fullName":["Pardigon N"],"firstName":["Nathalie"],"lastName":["Pardigon"],"initials":["N"],"affiliation":["Institut Pasteur, ERI/CIBU Arbovirus Group, 25 rue du Dr Roux, 75015 Paris, France."]},{"fullName":["Baffet AD"],"firstName":["Alexandre D"],"lastName":["Baffet"],"initials":["AD"],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France. Electronic address: alexandre.baffet@curie.fr."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2444983"],"dateOfPublication":["2016 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2016"],"printPublicationDate":["2016-08-01"],"journal":[{"title":["EBioMedicine"],"ISOAbbreviation":["EBioMedicine"],"medlineAbbreviation":["EBioMedicine"],"NLMid":["101647039"],"ESSN":["2352-3964"]}]}],"pubYear":["2016"],"pageInfo":["71-76"],"abstractText":["The recent Zika outbreak in South America and French Polynesia was associated with an epidemic of microcephaly, a disease characterized by a reduced size of the cerebral cortex. Other members of the Flavivirus genus, including West Nile virus (WNV), can cause encephalitis but were not demonstrated to cause microcephaly. It remains unclear whether Zika virus (ZIKV) and other flaviviruses may infect different cell populations in the developing neocortex and lead to distinct developmental defects. Here, we describe an assay to infect mouse E15 embryonic brain slices with ZIKV, WNV and dengue virus serotype 4 (DENV-4). We show that this tissue is able to support viral replication of ZIKV and WNV, but not DENV-4. Cell fate analysis reveals a remarkable tropism of ZIKV infection for neural stem cells. Closely related WNV displays a very different tropism of infection, with a bias towards neurons. We further show that ZIKV infection, but not WNV infection, impairs cell cycle progression of neural stem cells. Both viruses inhibited apoptosis at early stages of infection. This work establishes a powerful comparative approach to identify ZIKV-specific alterations in the developing neocortex and reveals specific preferential infection of neural stem cells by ZIKV."],"affiliation":["Institut Curie, PSL Research University, CNRS, UMR144, F-75005 Paris, France."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006693?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5006693"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.ebiom.2016.07.018"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-02"],"electronicPublicationDate":["2016-07-16"],"firstPublicationDate":["2016-07-16"],"cprojectID":"PMC5006693"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsDs_8VqgcuJwvkh","_score":1,"_source":{"id":["27532504"],"source":["MED"],"pmid":["27532504"],"pmcid":["PMC4988707"],"doi":["10.1371/journal.pone.0160928"],"title":["Multiple Modes of Action of the Squamocin in the Midgut Cells of Aedes aegypti Larvae."],"authorString":["da Silva Costa M, de Paula SO, Martins GF, Zanuncio JC, Santana AE, Serrão JE."],"authorList":[{"author":[{"fullName":["da Silva Costa M"],"firstName":["Marilza"],"lastName":["da Silva Costa"],"initials":["M"],"affiliation":["Department of Entomology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."]},{"fullName":["de Paula SO"],"firstName":["Sérgio Oliveira"],"lastName":["de Paula"],"initials":["SO"],"affiliation":["Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."]},{"fullName":["Martins GF"],"firstName":["Gustavo Ferreira"],"lastName":["Martins"],"initials":["GF"],"affiliation":["Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."]},{"fullName":["Zanuncio JC"],"firstName":["José Cola"],"lastName":["Zanuncio"],"initials":["JC"],"affiliation":["Department of Entomology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."]},{"fullName":["Santana AE"],"firstName":["Antônio Euzébio Goulart"],"lastName":["Santana"],"initials":["AE"],"affiliation":["Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas, Brazil."]},{"fullName":["Serrão JE"],"firstName":["José Eduardo"],"lastName":["Serrão"],"initials":["JE"],"affiliation":["Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."]}]}],"journalInfo":[{"issue":["8"],"volume":["11"],"journalIssueId":["2435494"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2016"],"pageInfo":["e0160928"],"abstractText":["Annonaceous acetogenins are botanical compounds with good potential for use as insecticides. In the vector, Aedes aegypti (L.) (Diptera: Culicidae), squamocin (acetogenin) has been reported to be a larvicide and cytotoxic, but the modes of action of this molecule are still poorly understood. This study evaluated the changes in the cell morphology, and in the expression of genes, for autophagy (Atg1 and Atg8), for membrane ion transporter V-ATPase, and for water channel aquaporin-4 (Aqp4) in the midgut of A. aegypti larvae exposed to squamocin from Annona mucosa Jacq. (Annonaceae). Squamocin showed cytotoxic action with changes in the midgut epithelium and digestive cells of A. aegypti larvae, increase in the expression for autophagy gene Atg1 and Atg8, decrease in the expression of V-ATPase, decrease in the expression of Aqp4 gene in LC20 and inhibition of Apq4 genes in the midgut of this vector in LC50. These multiple modes of action for squamocin are described for the first time in insects, and they are important because different sites of action of squamocin from A. mucosa may reduce the possibility of resistance of A. aegypti to this molecule."],"affiliation":["Department of Entomology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988707?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4988707"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0160928"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-18"],"dateOfRevision":["2016-08-31"],"electronicPublicationDate":["2016-08-17"],"firstPublicationDate":["2016-08-17"],"cprojectID":"PMC4988707"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrKPhOUS2orL1IPr","_score":1,"_source":{"id":["27070912"],"source":["MED"],"pmid":["27070912"],"pmcid":["PMC4829157"],"doi":["10.1371/journal.pntd.0004636"],"title":["Zika Virus Outbreak in Rio de Janeiro, Brazil: Clinical Characterization, Epidemiological and Virological Aspects."],"authorString":["Brasil P, Calvet GA, Siqueira AM, Wakimoto M, de Sequeira PC, Nobre A, Quintana Mde S, Mendonça MC, Lupi O, de Souza RV, Romero C, Zogbi H, Bressan Cda S, Alves SS, Lourenço-de-Oliveira R, Nogueira RM, Carvalho MS, de Filippis AM, Jaenisch T."],"authorList":[{"author":[{"fullName":["Brasil P"],"firstName":["Patrícia"],"lastName":["Brasil"],"initials":["P"],"authorId":[{"_":"0000-0001-9555-7976","$":{"type":"ORCID"}}],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Calvet GA"],"firstName":["Guilherme Amaral"],"lastName":["Calvet"],"initials":["GA"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Siqueira AM"],"firstName":["André Machado"],"lastName":["Siqueira"],"initials":["AM"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Wakimoto M"],"firstName":["Mayumi"],"lastName":["Wakimoto"],"initials":["M"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["de Sequeira PC"],"firstName":["Patrícia Carvalho"],"lastName":["de Sequeira"],"initials":["PC"],"affiliation":["Flavivirus Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Nobre A"],"firstName":["Aline"],"lastName":["Nobre"],"initials":["A"],"affiliation":["Scientific Computation Program, Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Quintana Mde S"],"firstName":["Marcel de Souza Borges"],"lastName":["Quintana"],"initials":["Mde S"],"affiliation":["Clinical Research Platform, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Mendonça MC"],"firstName":["Marco Cesar Lima de"],"lastName":["Mendonça"],"initials":["MC"],"affiliation":["Flavivirus Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Lupi O"],"firstName":["Otilia"],"lastName":["Lupi"],"initials":["O"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["de Souza RV"],"firstName":["Rogerio Valls"],"lastName":["de Souza"],"initials":["RV"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Romero C"],"firstName":["Carolina"],"lastName":["Romero"],"initials":["C"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Zogbi H"],"firstName":["Heruza"],"lastName":["Zogbi"],"initials":["H"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Bressan Cda S"],"firstName":["Clarisse da Silveira"],"lastName":["Bressan"],"initials":["Cda S"],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Alves SS"],"firstName":["Simone Sampaio"],"lastName":["Alves"],"initials":["SS"],"affiliation":["Flavivirus Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Lourenço-de-Oliveira R"],"firstName":["Ricardo"],"lastName":["Lourenço-de-Oliveira"],"initials":["R"],"affiliation":["Mosquito Transmitters of Hematozoans Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Nogueira RM"],"firstName":["Rita Maria Ribeiro"],"lastName":["Nogueira"],"initials":["RM"],"affiliation":["Flavivirus Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Carvalho MS"],"firstName":["Marilia Sá"],"lastName":["Carvalho"],"initials":["MS"],"affiliation":["Scientific Computation Program, Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["de Filippis AM"],"firstName":["Ana Maria Bispo"],"lastName":["de Filippis"],"initials":["AM"],"affiliation":["Flavivirus Laboratory, Oswaldo Cruz Institute/ Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."]},{"fullName":["Jaenisch T"],"firstName":["Thomas"],"lastName":["Jaenisch"],"initials":["T"],"affiliation":["Section Clinical Tropical Medicine, Department of Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany & German Center for Infectious Disease Research, Heidelberg, Germany."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9555-7976","$":{"type":"ORCID"}},{"_":"0000-0002-3545-5238","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004636"],"abstractText":["In 2015, Brazil was faced with the cocirculation of three arboviruses of major public health importance. The emergence of Zika virus (ZIKV) presents new challenges to both clinicians and public health authorities. Overlapping clinical features between diseases caused by ZIKV, Dengue (DENV) and Chikungunya (CHIKV) and the lack of validated serological assays for ZIKV make accurate diagnosis difficult.The outpatient service for acute febrile illnesses in Fiocruz initiated a syndromic clinical observational study in 2007 to capture unusual presentations of DENV infections. In January 2015, an increase of cases with exanthematic disease was observed. Trained physicians evaluated the patients using a detailed case report form that included clinical assessment and laboratory investigations. The laboratory diagnostic algorithm included assays for detection of ZIKV, CHIKV and DENV. 364 suspected cases of Zika virus disease were identified based on clinical criteria between January and July 2015. Of these, 262 (71.9%) were tested and 119 (45.4%) were confirmed by the detection of ZIKV RNA. All of the samples with sequence information available clustered within the Asian genotype.This is the first report of a ZIKV outbreak in the state of Rio de Janeiro, based on a large number of suspected (n = 364) and laboratory confirmed cases (n = 119). We were able to demonstrate that ZIKV was circulating in Rio de Janeiro as early as January 2015. The peak of the outbreak was documented in May/June 2015. More than half of the patients reported headache, arthralgia, myalgia, non-purulent conjunctivitis, and lower back pain, consistent with the case definition of suspected ZIKV disease issued by the Pan American Health Organization (PAHO). However, fever, when present, was low-intensity and short-termed. In our opinion, pruritus, the second most common clinical sign presented by the confirmed cases, should be added to the PAHO case definition, while fever could be given less emphasis. The emergence of ZIKV as a new pathogen for Brazil in 2015 underscores the need for clinical vigilance and strong epidemiological and laboratory surveillance."],"affiliation":["Acute Febrile Illnesses Laboratory, Evandro Chagas National Institute of Infectious Diseases; Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't","Observational Study"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Viral Envelope Proteins"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Cluster Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Analysis, DNA"]},{"majorTopic_YN":["Y"],"descriptorName":["Disease Outbreaks"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Sequence Homology"]},{"majorTopic_YN":["N"],"descriptorName":["Genotype"]},{"majorTopic_YN":["N"],"descriptorName":["Adolescent"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Middle Aged"]},{"majorTopic_YN":["N"],"descriptorName":["Child"]},{"majorTopic_YN":["N"],"descriptorName":["Brazil"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["Y"],"descriptorName":["Genetic Variation"]},{"majorTopic_YN":["N"],"descriptorName":["Young Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["Y"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]},{"name":["Viral Envelope Proteins"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4829157?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4829157"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004636"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["3"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-08"],"dateOfCreation":["2016-04-13"],"dateOfRevision":["2016-09-09"],"electronicPublicationDate":["2016-04-12"],"firstPublicationDate":["2016-04-12"],"cprojectID":"PMC4829157"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrNbJ9hGurOzZIZo","_score":1,"_source":{"id":["27105225"],"source":["MED"],"pmid":["27105225"],"pmcid":["PMC4841568"],"doi":["10.1371/journal.pntd.0004626"],"title":["Reproductive Incompatibility Involving Senegalese Aedes aegypti (L) Is Associated with Chromosome Rearrangements."],"authorString":["Dickson LB, Sharakhova MV, Timoshevskiy VA, Fleming KL, Caspary A, Sylla M, Black WC."],"authorList":[{"author":[{"fullName":["Dickson LB"],"firstName":["Laura B"],"lastName":["Dickson"],"initials":["LB"],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."]},{"fullName":["Sharakhova MV"],"firstName":["Maria V"],"lastName":["Sharakhova"],"initials":["MV"],"affiliation":["Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, Virginia, United States of America."]},{"fullName":["Timoshevskiy VA"],"firstName":["Vladimir A"],"lastName":["Timoshevskiy"],"initials":["VA"],"affiliation":["Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, Virginia, United States of America."]},{"fullName":["Fleming KL"],"firstName":["Karen L"],"lastName":["Fleming"],"initials":["KL"],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."]},{"fullName":["Caspary A"],"firstName":["Alex"],"lastName":["Caspary"],"initials":["A"],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."]},{"fullName":["Sylla M"],"firstName":["Massamba"],"lastName":["Sylla"],"initials":["M"],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."]},{"fullName":["Black WC 4th"],"firstName":["William C"],"lastName":["Black"],"initials":["WC"],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."]}]}],"journalInfo":[{"issue":["4"],"volume":["10"],"journalIssueId":["2392604"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2016"],"pageInfo":["e0004626"],"abstractText":["Aedes aegypti, the primary vector of dengue, yellow fever and Zika flaviviruses, consists of at least two subspecies. Aedes aegypti (Aaa) is light in color, has pale scales on the first abdominal tergite, oviposits in artificial containers, and preferentially feeds on humans. Aedes aegypti formosus (Aaf), has a dark cuticle, is restricted to sub-Saharan Africa, has no pale scales on the first abdominal tergite and frequently oviposits in natural containers. Scale patterns correlate with cuticle color in East Africa but not in Senegal, West Africa where black cuticle mosquitoes display a continuum of scaling patterns and breed domestically indoors. An earlier laboratory study did not indicate any pre- or postzygotic barriers to gene flow between Aaa and Aaf in East Africa. However, similar attempts to construct F1 intercross families between Aaa laboratory strains and Senegal Ae. aegypti (SenAae) failed due to poor F1 oviposition and low F2 egg-to-adult survival. Insemination and assortative mating experiments failed to identify prezygotic mating barriers. Backcrosses were performed to test for postzygotic isolation patterns consistent with Haldane's rule modified for species, like Aedes, that have an autosomal sex determining locus (SDL). Egg-pupal survival was predicted to be low in females mated to hybrid F1 males but average when a male mates with a hybrid F1 female. Survival was in fact significantly reduced when females mated to hybrid males but egg-pupal survival was significantly increased when males were mated to hybrid F1 females. These observations are therefore inconclusive with regards to Haldane's rule. Basic cytogenetic analyses and Fluorescent In Situ Hybridization (FISH) experiments were performed to compare SenAae strains with the IB12 strain of Aaa that was used for genome sequencing and physical mapping. Some SenAae strains had longer chromosomes than IB12 and significantly different centromeric indices on chromosomes 1 and 3. DAPI staining was used to identify AT-rich regions, chromomycin A3 following pretreatment with barium hydroxide stained for GC-rich regions and stained the ribosomal RNA locus and YOYO-1 was used to test for differential staining. Chromosome patterns in SenAae strains revealed by these three stains differed from those in IB12. For FISH, 40 BAC clones previously physically mapped on Aaa chromosomes were used to test for chromosome rearrangements in SenAae relative to IB12. Differences in the order of markers identified two chromosomal rearrangements between IB12 and SenAae strains. The first rearrangement involves two overlapping pericentric (containing the centromere) inversions in chromosome 3 or an insertion of a large fragment into the 3q arm. The second rearrangement is close to the centromere on the p arm of chromosome 2. Linkage analysis of the SDL and the white-eye locus identified a likely chromosomal rearrangement on chromosome 1. The reproductive incompatibility observed within SenAae and between SenAae and Aaa may be generally associated with chromosome rearrangements on all three chromosomes and specifically caused by pericentric inversions on chromosomes 2 and 3."],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Extramural"]}],"grantsList":[{"grant":[{"grantId":["R21AI121853"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["R01AI0833680"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Chromosome Aberrations"]},{"majorTopic_YN":["N"],"descriptorName":["Staining and Labeling"]},{"majorTopic_YN":["N"],"descriptorName":["In Situ Hybridization, Fluorescence"]},{"majorTopic_YN":["N"],"descriptorName":["Survival Analysis"]},{"majorTopic_YN":["N"],"descriptorName":["Crosses, Genetic"]},{"majorTopic_YN":["N"],"descriptorName":["Inbreeding"]},{"majorTopic_YN":["N"],"descriptorName":["Cytogenetics"]},{"majorTopic_YN":["N"],"descriptorName":["Reproduction"]},{"majorTopic_YN":["N"],"descriptorName":["Senegal"]},{"majorTopic_YN":["Y"],"descriptorName":["Chromosomes, Insect"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841568?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4841568"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004626"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-29"],"dateOfCreation":["2016-04-23"],"dateOfRevision":["2016-04-30"],"electronicPublicationDate":["2016-04-22"],"firstPublicationDate":["2016-04-22"],"cprojectID":"PMC4841568"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRIhOUS2orL1IQA","_score":1,"_source":{"id":["27195114"],"source":["MED"],"pmid":["27195114"],"pmcid":["PMC4855265"],"doi":["10.1038/cti.2016.15"],"title":["The cell surface environment for pathogen recognition and entry."],"authorString":["Stow JL, Condon ND."],"authorList":[{"author":[{"fullName":["Stow JL"],"firstName":["Jennifer L"],"lastName":["Stow"],"initials":["JL"],"affiliation":["IMB Centre for Inflammation and Disease Research, Institute for Molecular Bioscience, The University of Queensland , Brisbane, Queensland, Australia."]},{"fullName":["Condon ND"],"firstName":["Nicholas D"],"lastName":["Condon"],"initials":["ND"],"affiliation":["IMB Centre for Inflammation and Disease Research, Institute for Molecular Bioscience, The University of Queensland , Brisbane, Queensland, Australia."]}]}],"journalInfo":[{"issue":["4"],"volume":["5"],"journalIssueId":["2410032"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Clinical & translational immunology"],"ISOAbbreviation":["Clin Transl Immunology"],"medlineAbbreviation":["Clin Transl Immunology"],"NLMid":["101638268"],"ESSN":["2050-0068"]}]}],"pubYear":["2016"],"pageInfo":["e71"],"abstractText":["The surface of mammalian cells offers an interface between the cell interior and its surrounding milieu. As part of the innate immune system, macrophages have cell surface features optimised for probing and sampling as they patrol our tissues for pathogens, debris or dead cells. Their highly dynamic and constantly moving cell surface has extensions such as lamellipodia, filopodia and dorsal ruffles that help detect pathogens. Dorsal ruffles give rise to macropinosomes for rapid, high volume non-selective fluid sampling, receptor internalisation and plasma membrane turnover. Ruffles can also generate phagocytic cups for the receptor-mediated uptake of pathogens or particles. The membrane lipids, actin cytoskeleton, receptors and signalling proteins that constitute these cell surface domains are discussed. Although the cell surface is designed to counteract pathogens, many bacteria, viruses and other pathogens have evolved to circumvent or hijack these cell structures and their underlying machinery for entry and survival. Nevertheless, these features offer important potential for developing vaccines, drugs and preventative measures to help fight infection."],"affiliation":["IMB Centre for Inflammation and Disease Research, Institute for Molecular Bioscience, The University of Queensland , Brisbane, Queensland, Australia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4855265?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4855265"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/cti.2016.15"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-05-19"],"dateOfCreation":["2016-05-19"],"dateOfRevision":["2016-05-21"],"electronicPublicationDate":["2016-04-15"],"firstPublicationDate":["2016-04-15"],"cprojectID":"PMC4855265"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrRthOUS2orL1IQE","_score":1,"_source":{"id":["27158449"],"source":["MED"],"pmid":["27158449"],"pmcid":["PMC4857754"],"title":["Possible repurposing of seasonal influenza vaccine for prevention of Zika virus infection."],"authorString":["Veljkovic V, Paessler S."],"authorList":[{"author":[{"fullName":["Veljkovic V"],"firstName":["Veljko"],"lastName":["Veljkovic"],"initials":["V"],"affiliation":["Biomed Protection, Galveston, TX, USA."]},{"fullName":["Paessler S"],"firstName":["Slobodan"],"lastName":["Paessler"],"initials":["S"],"affiliation":["Department of Pathology, Galveston National Laboratory, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Institute for Human Infectious and Immunity, Galveston, TX, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["190"],"abstractText":["The in silico analysis shows that the envelope glycoproteins E of Zika viruses (ZIKV) isolated in Asia, Africa and South and Central America encode highly conserved information determining their interacting profile and immunological properties. Previously it was shown that the same information is encoded in the primary structure of the hemagglutinin subunit 1 (HA1) from pdmH1N1 influenza A virus. This similarity suggests possible repurposing of the seasonal influenza vaccine containing pdmH1N1 component for prevention of the ZIKV infection."],"affiliation":["Biomed Protection, Galveston, TX, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857754?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4857754"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["doi"]}],"dateOfCompletion":["2016-05-09"],"dateOfCreation":["2016-05-09"],"dateOfRevision":["2016-05-11"],"electronicPublicationDate":["2016-02-18"],"firstPublicationDate":["2016-02-18"],"cprojectID":"PMC4857754"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrS9_8VqgcuJwvh2","_score":1,"_source":{"id":["27093860"],"source":["MED"],"pmid":["27093860"],"pmcid":["PMC4837632"],"doi":["10.1186/s40249-016-0132-y"],"title":["The global spread of Zika virus: is public and media concern justified in regions currently unaffected?"],"authorString":["Gyawali N, Bradbury RS, Taylor-Robinson AW."],"authorList":[{"author":[{"fullName":["Gyawali N"],"firstName":["Narayan"],"lastName":["Gyawali"],"initials":["N"],"affiliation":["Infectious Diseases Research Group, School of Medical & Applied Sciences, Central Queensland University, Rockhampton, 4702, QLD, Australia."]},{"fullName":["Bradbury RS"],"firstName":["Richard S"],"lastName":["Bradbury"],"initials":["RS"],"affiliation":["Infectious Diseases Research Group, School of Medical & Applied Sciences, Central Queensland University, Rockhampton, 4702, QLD, Australia."]},{"fullName":["Taylor-Robinson AW"],"firstName":["Andrew W"],"lastName":["Taylor-Robinson"],"initials":["AW"],"affiliation":["Infectious Diseases Research Group, School of Medical & Applied Sciences, Central Queensland University, Rockhampton, 4702, QLD, Australia. a.taylor-robinson@cqu.edu.au."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2404967"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Infectious diseases of poverty"],"ISOAbbreviation":["Infect Dis Poverty"],"medlineAbbreviation":["Infect Dis Poverty"],"NLMid":["101606645"],"ESSN":["2049-9957"]}]}],"pubYear":["2016"],"pageInfo":["37"],"abstractText":["Zika virus, an Aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in Brazil.Prior to its emergence in Latin America in 2015-2016, Zika was known to exist at a relatively low prevalence in parts of Africa, Asia and the Pacific islands. An extension of its apparent global dispersion may be enabled by climate conditions suitable to support the population growth of A. aegypti and A. albopictus mosquitoes over an expanding geographical range. In addition, increased globalisation continues to pose a risk for the spread of infection. Further, suspicions of alternative modes of virus transmission (sexual and vertical), if proven, provide a platform for outbreaks in mosquito non-endemic regions as well. Since a vaccine or anti-viral therapy is not yet available, current means of disease prevention involve protection from mosquito bites, excluding pregnant females from travelling to Zika-endemic territories, and practicing safe sex in those countries. Importantly, in countries where Zika is reported as endemic, caution is advised in planning to conceive a baby until such time as the apparent association between infection with the virus and microcephaly is either confirmed or refuted. The question arises as to what advice is appropriate to give in more economically developed countries distant to the current epidemic and in which Zika has not yet been reported. Despite understandable concern among the general public that has been fuelled by the media, in regions where Zika is not present, such as North America, Europe and Australia, at this time any outbreak (initiated by an infected traveler returning from an endemic area) would very probably be contained locally. Since Aedes spp. has very limited spatial dispersal, overlapping high population densities of mosquitoes and humans would be needed to sustain a focus of infection. However, as A. aegypti is distinctly anthropophilic, future control strategies for Zika should be considered in tandem with the continuing threat to human wellbeing that is presented by dengue, yellow fever and Japanese encephalitis, all of which are transmitted by the same vector species."],"affiliation":["Infectious Diseases Research Group, School of Medical & Applied Sciences, Central Queensland University, Rockhampton, 4702, QLD, Australia."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Aedes","Transmission","Diagnosis","Treatment","Mosquito","epidemic","Arbovirus","Vector Control","Flavivirus","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837632?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4837632"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s40249-016-0132-y"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-20"],"dateOfRevision":["2016-04-22"],"electronicPublicationDate":["2016-04-19"],"firstPublicationDate":["2016-04-19"],"cprojectID":"PMC4837632"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrTZ_8VqgcuJwvh4","_score":1,"_source":{"id":["26850640"],"source":["MED"],"pmid":["26850640"],"pmcid":["PMC4838367"],"doi":["10.1093/nar/gkw061"],"title":["Kissing-loop interaction between 5' and 3' ends of tick-borne Langat virus genome 'bridges the gap' between mosquito- and tick-borne flaviviruses in mechanisms of viral RNA cyclization: applications for virus attenuation and vaccine development."],"authorString":["Tsetsarkin KA, Liu G, Shen K, Pletnev AG."],"authorList":[{"author":[{"fullName":["Tsetsarkin KA"],"firstName":["Konstantin A"],"lastName":["Tsetsarkin"],"initials":["KA"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, 20892-3203 USA."]},{"fullName":["Liu G"],"firstName":["Guangping"],"lastName":["Liu"],"initials":["G"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, 20892-3203 USA."]},{"fullName":["Shen K"],"firstName":["Kui"],"lastName":["Shen"],"initials":["K"],"affiliation":["Bioinformatics and Computational Biosciences Branch, NIAID, NIH, Bethesda, MD, 20892, USA."]},{"fullName":["Pletnev AG"],"firstName":["Alexander G"],"lastName":["Pletnev"],"initials":["AG"],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, 20892-3203 USA apletnev@niaid.nih.gov."]}]}],"journalInfo":[{"issue":["7"],"volume":["44"],"journalIssueId":["2400260"],"dateOfPublication":["2016 Apr"],"monthOfPublication":["4"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Nucleic acids research"],"ISOAbbreviation":["Nucleic Acids Res."],"medlineAbbreviation":["Nucleic Acids Res"],"NLMid":["0411011"],"ISSN":["0305-1048"],"ESSN":["1362-4962"]}]}],"pubYear":["2016"],"pageInfo":["3330-3350"],"abstractText":["Insertion of microRNA target sequences into the flavivirus genome results in selective tissue-specific attenuation and host-range restriction of live attenuated vaccine viruses. However, previous strategies for miRNA-targeting did not incorporate a mechanism to prevent target elimination under miRNA-mediated selective pressure, restricting their use in vaccine development. To overcome this limitation, we developed a new approach for miRNA-targeting of tick-borne flavivirus (Langat virus, LGTV) in the duplicated capsid gene region (DCGR). Genetic stability of viruses with DCGR was ensured by the presence of multiple cis-acting elements within the N-terminal capsid coding region, including the stem-loop structure (5'SL6) at the 3' end of the promoter. We found that the 5'SL6 functions as a structural scaffold for the conserved hexanucleotide motif at its tip and engages in a complementary interaction with the region present in the 3' NCR to enhance viral RNA replication. The resulting kissing-loop interaction, common in tick-borne flaviviruses, supports a single pair of cyclization elements (CYC) and functions as a homolog of the second pair of CYC that is present in the majority of mosquito-borne flaviviruses. Placing miRNA targets into the DCGR results in superior attenuation of LGTV in the CNS and does not interfere with development of protective immunity in immunized mice."],"affiliation":["Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD, 20892-3203 USA."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, N.I.H., Intramural"]}],"grantsList":[{"grant":[{"agency":["Intramural NIH HHS"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4838367?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4838367"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/nar/gkw061"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCreation":["2016-04-21"],"dateOfRevision":["2016-04-22"],"electronicPublicationDate":["2016-02-04"],"firstPublicationDate":["2016-02-04"],"cprojectID":"PMC4838367"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrTcJ9hGurOzZIZ6","_score":1,"_source":{"id":["27154247"],"source":["MED"],"pmid":["27154247"],"pmcid":["PMC4859974"],"doi":["10.1186/s12985-016-0534-2"],"title":["Dengue research: a bibliometric analysis of worldwide and Arab publications during 1872-2015."],"authorString":["Zyoud SH."],"authorList":[{"author":[{"fullName":["Zyoud SH"],"firstName":["Sa'ed H"],"lastName":["Zyoud"],"initials":["SH"],"affiliation":["Department of Clinical and Community Pharmacy, College of Medicine and Health Sciences, An-Najah National University, Nablus, 44839, Palestine. saedzyoud@yahoo.com."]}]}],"journalInfo":[{"volume":["13"],"journalIssueId":["2401989"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Virology journal"],"ISOAbbreviation":["Virol. J."],"medlineAbbreviation":["Virol J"],"NLMid":["101231645"],"ESSN":["1743-422X"]}]}],"pubYear":["2016"],"pageInfo":["78"],"abstractText":["Dengue is an important emerging and re-emerging arboviral infection globally as a rapidly growing and widespread public health problem, with transmission occurring in more than 128 countries in Asia, Americas, southeast Africa, western Pacific, and eastern Mediterranean regions. Therefore, the aim of this study was to characterize and quantify the scientific output of dengue research in Arab countries relative to that worldwide by using a bibliometric analysis.The standardized search approach based on the use of the the keyword \"dengue\" in the title, abstract, and keyword field was used to get research output related to dengue at a global level. All data related to dengue were collected from the past to December 31, 2015.A total of 19,581 dengue-related documents identified in the Scopus database. The results show that the study of dengue exhibits an overall upward trend from 1872 to 2015 with peak publications in 2014. The leading countries in dengue research were the USA (4,709; 24.05 %), India (1,942; 9.92 %), Brazil (1,530; 7.81 %), Thailand (1,260; 6.43 %), the UK (1,129; 5.77 %), and France (1,087; 5.55 %). Only 226 (1.16 % of the overall global research effort in the dengue field) articles were published from the Arab region. The total number of citations for all publications was 352,710, with an average of 18.0 citations per publication. Furthermore, the h-index for all extracted data related to dengue research was 186. Kingdom of Saudi Arabia (KSA) was the most productive country in Arab region with 102 documents representing 45.1 %. Furthermore, the h-index for all extracted data related to dengue research was 27. The USA was Arab's most main cooperative partner (46, 20.4 %), followed by India (36, 15.9 %).The amount of literature related to dengue research has considerably increased over the last decade. This bibliometric analysis has demonstrated the leading role that the USA, India, Brazil, Thailand, the UK, and France play in dengue research. The Arab world produced fewer publications related to dengue with lower quality than other world countries."],"affiliation":["Department of Clinical and Community Pharmacy, College of Medicine and Health Sciences, An-Najah National University, Nablus, 44839, Palestine. saedzyoud@yahoo.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Dengue","Citations","Scopus","Bibliometric","Arab World"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4859974?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4859974"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12985-016-0534-2"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-07"],"dateOfRevision":["2016-05-09"],"electronicPublicationDate":["2016-05-06"],"firstPublicationDate":["2016-05-06"],"cprojectID":"PMC4859974"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrWihOUS2orL1IQL","_score":1,"_source":{"id":["27149616"],"source":["MED"],"pmid":["27149616"],"pmcid":["PMC4858160"],"doi":["10.1371/journal.pgen.1005986"],"title":["Exploiting Genetic Interference for Antiviral Therapy."],"authorString":["Tanner EJ, Kirkegaard KA, Weinberger LS."],"authorList":[{"author":[{"fullName":["Tanner EJ"],"firstName":["Elizabeth J"],"lastName":["Tanner"],"initials":["EJ"],"authorId":[{"_":"0000-0001-9445-6620","$":{"type":"ORCID"}}],"affiliation":["Gladstone Institutes (Virology and Immunology), San Francisco, California, United States of America."]},{"fullName":["Kirkegaard KA"],"firstName":["Karla A"],"lastName":["Kirkegaard"],"initials":["KA"],"affiliation":["Department of Genetics, Stanford University School of Medicine, Stanford, California, United States of America."]},{"fullName":["Weinberger LS"],"firstName":["Leor S"],"lastName":["Weinberger"],"initials":["LS"],"affiliation":["Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, United States of America."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-9445-6620","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["12"],"journalIssueId":["2403386"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["PLoS genetics"],"ISOAbbreviation":["PLoS Genet."],"medlineAbbreviation":["PLoS Genet"],"NLMid":["101239074"],"ISSN":["1553-7390"],"ESSN":["1553-7404"]}]}],"pubYear":["2016"],"pageInfo":["e1005986"],"abstractText":["Rapidly evolving viruses are a major threat to human health. Such viruses are often highly pathogenic (e.g., influenza virus, HIV, Ebola virus) and routinely circumvent therapeutic intervention through mutational escape. Error-prone genome replication generates heterogeneous viral populations that rapidly adapt to new selection pressures, leading to resistance that emerges with treatment. However, population heterogeneity bears a cost: when multiple viral variants replicate within a cell, they can potentially interfere with each other, lowering viral fitness. This genetic interference can be exploited for antiviral strategies, either by taking advantage of a virus's inherent genetic diversity or through generating de novo interference by engineering a competing genome. Here, we discuss two such antiviral strategies, dominant drug targeting and therapeutic interfering particles. Both strategies harness the power of genetic interference to surmount two particularly vexing obstacles-the evolution of drug resistance and targeting therapy to high-risk populations-both of which impede treatment in resource-poor settings."],"affiliation":["Gladstone Institutes (Virology and Immunology), San Francisco, California, United States of America."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"grantsList":[{"grant":[{"grantId":["DP1 DE024408"],"agency":["NIDCR NIH HHS"],"acronym":["DE"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858160?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858160"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pgen.1005986"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-06"],"dateOfRevision":["2016-07-01"],"electronicPublicationDate":["2016-05-05"],"firstPublicationDate":["2016-05-05"],"cprojectID":"PMC4858160"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrWqhOUS2orL1IQN","_score":1,"_source":{"id":["27088243"],"source":["MED"],"pmid":["27088243"],"pmcid":["PMC4861506"],"doi":["10.3201/eid2205.150547"],"title":["Tropical Islands as New Hubs for Emerging Arboviruses."],"authorString":["Cao-Lormeau VM."],"authorList":[{"author":[{"fullName":["Cao-Lormeau VM"],"firstName":["Van-Mai"],"lastName":["Cao-Lormeau"],"initials":["VM"]}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["913-915"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Letter"]}],"keywordList":[{"keyword":["Viruses","Islands","Tropical","Arboviruses","Pacific","Indian ocean","Dengue","Caribbean","Chikungunya","Vector-borne Infections","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861506?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861506"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.150547"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861506"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXEJ9hGurOzZIZ7","_score":1,"_source":{"id":["27089008"],"source":["MED"],"pmid":["27089008"],"pmcid":["PMC4861526"],"doi":["10.3201/eid2205.151875"],"title":["Spectrum of Viral Pathogens in Blood of Malaria-Free Ill Travelers Returning to Canada."],"authorString":["Kariyawasam R, Lau R, Eshaghi A, Patel SN, Sider D, Gubbay JB, Boggild AK."],"authorList":[{"author":[{"fullName":["Kariyawasam R"],"firstName":["Ruwandi"],"lastName":["Kariyawasam"],"initials":["R"]},{"fullName":["Lau R"],"firstName":["Rachel"],"lastName":["Lau"],"initials":["R"]},{"fullName":["Eshaghi A"],"firstName":["Alireza"],"lastName":["Eshaghi"],"initials":["A"]},{"fullName":["Patel SN"],"firstName":["Samir N"],"lastName":["Patel"],"initials":["SN"]},{"fullName":["Sider D"],"firstName":["Doug"],"lastName":["Sider"],"initials":["D"]},{"fullName":["Gubbay JB"],"firstName":["Jonathan B"],"lastName":["Gubbay"],"initials":["JB"]},{"fullName":["Boggild AK"],"firstName":["Andrea K"],"lastName":["Boggild"],"initials":["AK"]}]}],"journalInfo":[{"issue":["5"],"volume":["22"],"journalIssueId":["2399418"],"dateOfPublication":["2016 May"],"monthOfPublication":["5"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2016"],"pageInfo":["854-861"],"abstractText":["Malaria is the most common specific cause of fever in returning travelers, but many other vectorborne infections and viral infections are emerging and increasingly encountered by travelers. We documented common and emerging viral pathogens in malaria-negative specimens from ill travelers returning to Canada. Anonymized, malaria-negative specimens were examined for various viral pathogens by real-time PCR. Samples were positive for herpes simplex viruses 1 or 2 (n = 21, 1.6%), cytomegalovirus (n = 4, 0.3%), Epstein-Barr virus (n = 194, 14.9%), dengue virus types 1-4 (n = 27, 2.1%), chikungunya virus (n = 5, 0.4%), and hepatitis A virus (n = 12, 0.9%). Travel-acquired viral pathogens were documented in >20% of malaria-negative specimens, of which 2.5% were infected with dengue and chikungunya viruses. Our findings support the anecdotal impression that these vectorborne pathogens are emerging among persons who travel from Canada to other countries."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"keywordList":[{"keyword":["Viruses","Dengue virus","Chikungunya virus","Canada","pathogens","Tropical Medicine","Travel Medicine","Flaviviruses","Flaviviridae","Togaviridae","Vectorborne Infections","Fever In Returned Travelers","Imported Viral Infections","Travel-acquired Viral Pathogens"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861526?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4861526"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2205.151875"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-19"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-05-01"],"cprojectID":"PMC4861526"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrXr_8VqgcuJwviG","_score":1,"_source":{"id":["27056346"],"source":["MED"],"pmid":["27056346"],"pmcid":["PMC4854088"],"doi":["10.4103/1008-682x.179161"],"title":["Current updates on laboratory techniques for the diagnosis of male reproductive failure."],"authorString":["Sikka SC, Hellstrom WJ."],"authorList":[{"author":[{"fullName":["Sikka SC"],"firstName":["Suresh C"],"lastName":["Sikka"],"initials":["SC"],"affiliation":["Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana, USA."]},{"fullName":["Hellstrom WJ"],"firstName":["Wayne J G"],"lastName":["Hellstrom"],"initials":["WJ"]}]}],"journalInfo":[{"issue":["3"],"volume":["18"],"journalIssueId":["2400167"],"dateOfPublication":["2016 May-Jun"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-05-01"],"journal":[{"title":["Asian journal of andrology"],"ISOAbbreviation":["Asian J. Androl."],"medlineAbbreviation":["Asian J Androl"],"NLMid":["100942132"],"ISSN":["1008-682X"],"ESSN":["1745-7262"]}]}],"pubYear":["2016"],"pageInfo":["392-401"],"abstractText":["The incidence of male reproductive failure leading to infertility, whether due to delayed parenthood, environmental issues, genetic factors, drugs, etc., is increasing throughout the world. The diagnosis and prognosis of male subfertility have become a challenge. While the basic semen assessment has been performed for many years, a number of studies question the value of the traditional semen characteristics. This is partly due to inadequate methods and standardization, limited knowledge of technical requirements for quality assurance, and an incomplete understanding of what clinical information a semen assessment can provide. Laboratories currently performing semen and endocrine assessment show great variability. The World Health Organization (WHO) manual for the evaluation of semen has been the core of andrology and fertility evaluation that has helped in further development of this field over many years. These include the physical appearance of the ejaculate, assessments of sperm count, motility, vitality, morphology, and functional aspects of the sperm and semen sample. These tests also include male endocrine profile, biochemical evaluation of the semen, detection of antisperm antibodies in serum, the use of computer-aided sperm analysis (CASA), sperm DNA integrity, and its damage due to oxidative stress. Assisted reproductive techniques (e.g., IVF, ICSI) have shown great success but are too expensive. Further development in this field with newer techniques and extensive training/instructions can improve accuracy and reduce variability, thus maintaining the quality and standards of such an evaluation. There is an urgent need to have standardized training centers and increased awareness in this area of men's health for reproductive success."],"affiliation":["Department of Urology, Tulane University School of Medicine, New Orleans, Louisiana, USA."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4854088?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4854088"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4103/1008-682X.179161"]}]}],"commentCorrectionList":[{"commentCorrection":[{"id":["27048790"],"source":["MED"],"reference":["Asian J Androl. 2016 May-Jun;18(3):402"],"type":["Comment in"],"orderIn":["74"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-04-21"],"dateOfRevision":["2016-05-11"],"firstPublicationDate":["2016-04-08"],"cprojectID":"PMC4854088"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrsP_8VqgcuJwvjV","_score":1,"_source":{"id":["27413690"],"source":["MED"],"pmid":["27413690"],"pmcid":["PMC4925901"],"doi":["10.1016/j.idcr.2016.06.003"],"title":["Fatal leptospirosis and chikungunya co-infection: Do not forget leptospirosis during chikungunya outbreaks."],"authorString":["Nhan TX, Bonnieux E, Rovery C, De Pina JJ, Musso D."],"authorList":[{"author":[{"fullName":["Nhan TX"],"firstName":["Tu-Xuan"],"lastName":["Nhan"],"initials":["TX"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]},{"fullName":["Bonnieux E"],"firstName":["Eric"],"lastName":["Bonnieux"],"initials":["E"],"affiliation":["Unité de réanimation, Centre Hospitalier Territorial, Pirae, Tahiti, French Polynesia."]},{"fullName":["Rovery C"],"firstName":["Clarisse"],"lastName":["Rovery"],"initials":["C"],"affiliation":["Centre Hospitalier d'Uturoa, Raiata, French Polynesia."]},{"fullName":["De Pina JJ"],"firstName":["Jean-Jacques"],"lastName":["De Pina"],"initials":["JJ"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]},{"fullName":["Musso D"],"firstName":["Didier"],"lastName":["Musso"],"initials":["D"],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2422930"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["IDCases"],"ISOAbbreviation":["IDCases"],"medlineAbbreviation":["IDCases"],"NLMid":["101634540"],"ESSN":["2214-2509"]}]}],"pubYear":["2016"],"pageInfo":["12-14"],"abstractText":["In endemic areas, leptospirosis can be missed by erroneous clinical or laboratory diagnosis of arboviroses or co-infections with arboviruses and an increase in mortality due to leptospirosis has already been reported during arboviruses outbreaks. During the French Polynesian chikungunya virus outbreak in 2014-2015, two leptospirosis and chikungunya co-infections were reported, one of which was fatal. Diagnosis of leptospiroses was delayed in the context of chikungunya outbreak. In the context of arbovirus outbreak, the risk of misdiagnosis of leptospirosis is maximum and clinicians should initiate early antibiotic therapy if leptospirosis is suspected. A delayed diagnosis of leptospirosis can be responsible for fatal outcome. Leptospirosis should be considered even if dengue or chikungunya virus infections are confirmed by reference molecular testing."],"affiliation":["Institut Louis Malardé, Papeete, Tahiti, French Polynesia."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Leptospirosis","outbreak","Dengue","French Polynesia","Chikungunya"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4925901?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4925901"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.idcr.2016.06.003"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-14"],"dateOfCreation":["2016-07-14"],"dateOfRevision":["2016-07-16"],"electronicPublicationDate":["2016-06-22"],"firstPublicationDate":["2016-06-22"],"cprojectID":"PMC4925901"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrsbhOUS2orL1IRk","_score":1,"_source":{"id":["27508060"],"source":["MED"],"pmid":["27508060"],"pmcid":["PMC4957174"],"title":["search.bioPreprint: a discovery tool for cutting edge, preprint biomedical research articles."],"authorString":["Iwema CL, LaDue J, Zack A, Chattopadhyay A."],"authorList":[{"author":[{"fullName":["Iwema CL"],"firstName":["Carrie L"],"lastName":["Iwema"],"initials":["CL"],"affiliation":["Health Sciences Library System, University of Pittsburgh, Pittsburgh, USA."]},{"fullName":["LaDue J"],"firstName":["John"],"lastName":["LaDue"],"initials":["J"],"affiliation":["Health Sciences Library System, University of Pittsburgh, Pittsburgh, USA."]},{"fullName":["Zack A"],"firstName":["Angela"],"lastName":["Zack"],"initials":["A"],"affiliation":["Health Sciences Library System, University of Pittsburgh, Pittsburgh, USA."]},{"fullName":["Chattopadhyay A"],"firstName":["Ansuman"],"lastName":["Chattopadhyay"],"initials":["A"],"affiliation":["Health Sciences Library System, University of Pittsburgh, Pittsburgh, USA."]}]}],"journalInfo":[{"volume":["5"],"journalIssueId":["2391209"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["F1000Research"],"ISOAbbreviation":["F1000Res"],"medlineAbbreviation":["F1000Res"],"NLMid":["101594320"],"ESSN":["2046-1402"]}]}],"pubYear":["2016"],"pageInfo":["1396"],"abstractText":["The time it takes for a completed manuscript to be published traditionally can be extremely lengthy. Article publication delay, which occurs in part due to constraints associated with peer review, can prevent the timely dissemination of critical and actionable data associated with new information on rare diseases or developing health concerns such as Zika virus. Preprint servers are open access online repositories housing preprint research articles that enable authors (1) to make their research immediately and freely available and (2) to receive commentary and peer review prior to journal submission. There is a growing movement of preprint advocates aiming to change the current journal publication and peer review system, proposing that preprints catalyze biomedical discovery, support career advancement, and improve scientific communication. While the number of articles submitted to and hosted by preprint servers are gradually increasing, there has been no simple way to identify biomedical research published in a preprint format, as they are not typically indexed and are only discoverable by directly searching the specific preprint server websites. To address this issue, we created a search engine that quickly compiles preprints from disparate host repositories and provides a one-stop search solution. Additionally, we developed a web application that bolsters the discovery of preprints by enabling each and every word or phrase appearing to with articles from preprint servers. This tool, search.bioPreprint, is publicly available at http://www.hsls.pitt.edu/resources/preprint."],"affiliation":["Health Sciences Library System, University of Pittsburgh, Pittsburgh, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957174?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4957174"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-10"],"dateOfCreation":["2016-08-10"],"electronicPublicationDate":["2016-06-16"],"firstPublicationDate":["2016-06-16"],"cprojectID":"PMC4957174"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrspJ9hGurOzZIbT","_score":1,"_source":{"id":["27412284"],"source":["MED"],"pmid":["27412284"],"pmcid":["PMC4947883"],"doi":["10.1098/rspb.2016.0298"],"title":["Larval food quantity affects the capacity of adult mosquitoes to transmit human malaria."],"authorString":["Shapiro LL, Murdock CC, Jacobs GR, Thomas RJ, Thomas MB."],"authorList":[{"author":[{"fullName":["Shapiro LL"],"firstName":["Lillian L M"],"lastName":["Shapiro"],"initials":["LL"],"affiliation":["Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA llmshapiro@gmail.com."]},{"fullName":["Murdock CC"],"firstName":["Courtney C"],"lastName":["Murdock"],"initials":["CC"],"affiliation":["College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA Odum School of Ecology, University of Georgia, Athens, GA 30602, USA."]},{"fullName":["Jacobs GR"],"firstName":["Gregory R"],"lastName":["Jacobs"],"initials":["GR"],"affiliation":["Odum School of Ecology, University of Georgia, Athens, GA 30602, USA."]},{"fullName":["Thomas RJ"],"firstName":["Rachel J"],"lastName":["Thomas"],"initials":["RJ"],"affiliation":["Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA."]},{"fullName":["Thomas MB"],"firstName":["Matthew B"],"lastName":["Thomas"],"initials":["MB"],"affiliation":["Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA."]}]}],"journalInfo":[{"issue":["1834"],"volume":["283"],"journalIssueId":["2427140"],"dateOfPublication":["2016 Jul"],"monthOfPublication":["7"],"yearOfPublication":["2016"],"printPublicationDate":["2016-07-01"],"journal":[{"title":["Proceedings. Biological sciences / The Royal Society"],"ISOAbbreviation":["Proc. Biol. Sci."],"medlineAbbreviation":["Proc Biol Sci"],"NLMid":["101245157"],"ISSN":["0962-8452"],"ESSN":["1471-2954"]}]}],"pubYear":["2016"],"abstractText":["Adult traits of holometabolous insects are shaped by conditions experienced during larval development, which might impact interactions between adult insect hosts and parasites. However, the ecology of larval insects that vector disease remains poorly understood. Here, we used Anopheles stephensi mosquitoes and the human malaria parasite Plasmodium falciparum, to investigate whether larval conditions affect the capacity of adult mosquitoes to transmit malaria. We reared larvae in two groups; one group received a standard laboratory rearing diet, whereas the other received a reduced diet. Emerging adult females were then provided an infectious blood meal. We assessed mosquito longevity, parasite development rate and prevalence of infectious mosquitoes over time. Reduced larval food led to increased adult mortality and caused a delay in parasite development and a slowing in the rate at which parasites invaded the mosquito salivary glands, extending the time it took for mosquitoes to become infectious. Together, these effects increased transmission potential of mosquitoes in the high food regime by 260-330%. Such effects have not, to our knowledge, been shown previously for human malaria and highlight the importance of improving knowledge of larval ecology to better understand vector-borne disease transmission dynamics."],"affiliation":["Department of Entomology, The Pennsylvania State University, University Park, PA 16802, USA Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA 16802, USA llmshapiro@gmail.com."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Plasmodium falciparum","Anopheles","Vector Competence","Vector Borne Disease","Vectorial Capacity","Extrinsic Incubation Period"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947883?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4947883"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1098/rspb.2016.0298"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["Y"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-14"],"dateOfRevision":["2016-07-19"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4947883"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrvihOUS2orL1IRv","_score":1,"_source":{"id":["27465199"],"source":["MED"],"pmid":["27465199"],"pmcid":["PMC4963934"],"doi":["10.1186/s13071-016-1709-9"],"title":["The use of mosquito repellents at three sites in India with declining malaria transmission: surveys in the community and clinic."],"authorString":["van Eijk AM, Ramanathapuram L, Sutton PL, Peddy N, Choubey S, Mohanty S, Asokan A, Ravishankaran S, Priya GS, Johnson JA, Velayutham S, Kanagaraj D, Patel A, Desai N, Tandel N, Sullivan SA, Wassmer SC, Singh R, Pradhan K, Carlton JM, Srivasatava HC, Eapen A, Sharma SK."],"authorList":[{"author":[{"fullName":["van Eijk AM"],"firstName":["Anna Maria"],"lastName":["van Eijk"],"initials":["AM"],"authorId":[{"_":"0000-0003-1635-1289","$":{"type":"ORCID"}}],"affiliation":["Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA. amvaneijk@gmail.com."]},{"fullName":["Ramanathapuram L"],"firstName":["Lalitha"],"lastName":["Ramanathapuram"],"initials":["L"],"affiliation":["Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA."]},{"fullName":["Sutton PL"],"firstName":["Patrick L"],"lastName":["Sutton"],"initials":["PL"],"affiliation":["Acsel Health, 500 5th Ave, Suite 2760, New York, NY, 10110, USA."]},{"fullName":["Peddy N"],"firstName":["Nandini"],"lastName":["Peddy"],"initials":["N"],"affiliation":["Jigyansha, International Center of Excellence for Malaria Research, Sector 1, Raurkela, Odisha, India."]},{"fullName":["Choubey S"],"firstName":["Sandhya"],"lastName":["Choubey"],"initials":["S"],"affiliation":["Jigyansha, International Center of Excellence for Malaria Research, Sector 1, Raurkela, Odisha, India."]},{"fullName":["Mohanty S"],"firstName":["Stuti"],"lastName":["Mohanty"],"initials":["S"],"affiliation":["Jigyansha, International Center of Excellence for Malaria Research, Sector 1, Raurkela, Odisha, India."]},{"fullName":["Asokan A"],"firstName":["Aswin"],"lastName":["Asokan"],"initials":["A"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Ravishankaran S"],"firstName":["Sangamithra"],"lastName":["Ravishankaran"],"initials":["S"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Priya GS"],"firstName":["G Sri Lakshmi"],"lastName":["Priya"],"initials":["GS"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Johnson JA"],"firstName":["Justin Amala"],"lastName":["Johnson"],"initials":["JA"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Velayutham S"],"firstName":["Sangeetha"],"lastName":["Velayutham"],"initials":["S"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Kanagaraj D"],"firstName":["Deena"],"lastName":["Kanagaraj"],"initials":["D"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Patel A"],"firstName":["Ankita"],"lastName":["Patel"],"initials":["A"],"affiliation":["National Institute of Malaria Research Field Unit, Civil Hospital, Nadiad, Gujarat, India."]},{"fullName":["Desai N"],"firstName":["Nisha"],"lastName":["Desai"],"initials":["N"],"affiliation":["National Institute of Malaria Research Field Unit, Civil Hospital, Nadiad, Gujarat, India."]},{"fullName":["Tandel N"],"firstName":["Nikunj"],"lastName":["Tandel"],"initials":["N"],"affiliation":["National Institute of Malaria Research Field Unit, Civil Hospital, Nadiad, Gujarat, India."]},{"fullName":["Sullivan SA"],"firstName":["Steven A"],"lastName":["Sullivan"],"initials":["SA"],"affiliation":["Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA."]},{"fullName":["Wassmer SC"],"firstName":["Samuel C"],"lastName":["Wassmer"],"initials":["SC"],"affiliation":["London School of Hygiene and Tropical Medicine, Keppel St, London, WC1E 7HT, UK."]},{"fullName":["Singh R"],"firstName":["Ranveer"],"lastName":["Singh"],"initials":["R"],"affiliation":["National Institute of Malaria Research Field Unit, Civil Hospital, Nadiad, Gujarat, India."]},{"fullName":["Pradhan K"],"firstName":["K"],"lastName":["Pradhan"],"initials":["K"],"affiliation":["Jigyansha, International Center of Excellence for Malaria Research, Sector 1, Raurkela, Odisha, India."]},{"fullName":["Carlton JM"],"firstName":["Jane M"],"lastName":["Carlton"],"initials":["JM"],"affiliation":["Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA."]},{"fullName":["Srivasatava HC"],"firstName":["H C"],"lastName":["Srivasatava"],"initials":["HC"],"affiliation":["National Institute of Malaria Research Field Unit, Civil Hospital, Nadiad, Gujarat, India."]},{"fullName":["Eapen A"],"firstName":["Alex"],"lastName":["Eapen"],"initials":["A"],"affiliation":["National Institute of Malaria Research Field Unit, Indian Council of Medical Research, National Institute of Epidemiology Campus, Ayapakkam, Chennai, Tamil Nadu, India."]},{"fullName":["Sharma SK"],"firstName":["S K"],"lastName":["Sharma"],"initials":["SK"],"affiliation":["National Institute of Malaria Research, Indian Council of Medical Research, Dwarka Sector 8, New Delhi, India."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0003-1635-1289","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["418"],"abstractText":["Repellents such as coils, vaporizers, mats and creams can be used to reduce the risk of malaria and other infectious diseases. Although evidence for their effectiveness is limited, they are advertised as providing an additional approach to mosquito control in combination with other strategies, e.g. insecticide-treated nets. We examined the use of repellents in India in an urban setting in Chennai (mainly Plasmodium vivax malaria), a peri-urban setting in Nadiad (both P. vivax and P. falciparum malaria), and a more rural setting in Raurkela (mainly P. falciparum malaria).The use of repellents was examined at the household level during a census, and at the individual level in cross-sectional surveys and among patients visiting a clinic with fever or other symptoms. Factors associated with their use were examined in a multivariate analysis, and the association between malaria and the use of repellents was assessed among survey- and clinic participants.Characteristics of participants differed by region, with more people of higher education present in Chennai. Use of repellents varied between 56-77 % at the household level and between 32-78 % at the individual level. Vaporizers were the main repellents used in Chennai, whereas coils were more common in Nadiad and Raurkela. In Chennai and Nadiad, vaporizers were more likely to be used in households with young male children. Vaporizer use was associated with higher socio-economic status (SES) in households in Chennai and Nadiad, whereas use of coils was greater in the lower SES strata. In Raurkela, there was a higher use of coils among the higher SES strata. Education was associated with the use of a repellent among survey participants in Chennai and clinic study participants in Chennai and Nadiad. Repellent use was associated with less malaria in the clinic study in Chennai and Raurkela, but not in the surveys, with the exception of the use of coils in Nadiad.Repellents are widely used in India. Their use is influenced by the level of education and SES. Information on effectiveness and guidance on choices may improve rational use."],"affiliation":["Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, 10003, USA. amvaneijk@gmail.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Plasmodium falciparum","Urban","Mosquito control","Plasmodium vivax","Repellents","Rural","Education","Socio-economic Status"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963934?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963934"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1709-9"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-28"],"dateOfRevision":["2016-07-30"],"electronicPublicationDate":["2016-07-27"],"firstPublicationDate":["2016-07-27"],"cprojectID":"PMC4963934"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwTJ9hGurOzZIbk","_score":1,"_source":{"id":["27482459"],"source":["MED"],"pmid":["27482459"],"pmcid":["PMC4967971"],"title":["The difficulties of 'living while girl'."],"authorString":["Bruce J."],"authorList":[{"author":[{"fullName":["Bruce J"],"firstName":["Judith"],"lastName":["Bruce"],"initials":["J"],"affiliation":["Population Council , New York , USA."]}]}],"journalInfo":[{"issue":["3"],"volume":["2"],"journalIssueId":["2435853"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Journal of virus eradication"],"ISOAbbreviation":["J Virus Erad"],"medlineAbbreviation":["J Virus Erad"],"NLMid":["101654142"],"ISSN":["2055-6640"],"ESSN":["2055-6659"]}]}],"pubYear":["2016"],"pageInfo":["177-182"],"abstractText":["In this Viewpoint, Judith Bruce answers questions from Journal of Virus Eradication Editor, Sabine Kinloch-de Loës, on the importance of fulfilling the basic human rights of adolescent girls and their relationship with viral epidemics such as HIV. Judith Bruce is a graduate of Harvard University and a Senior Associate and Policy Analyst at the Population Council, New York, USA, whose work is aimed at building the health, social and cognitive assets of girls in the poorest communities in the developing world."],"affiliation":["Population Council , New York , USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["HIV infection","Adolescents","Human Rights","Girls","Child Marriage","Sexual Exploitation"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967971?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4967971"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-04"],"electronicPublicationDate":["2016-07-01"],"firstPublicationDate":["2016-07-01"],"cprojectID":"PMC4967971"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrwZhOUS2orL1IR0","_score":1,"_source":{"id":["27449206"],"source":["MED"],"pmid":["27449206"],"pmcid":["PMC4969310"],"doi":["10.1016/j.jaim.2016.07.002"],"title":["Strengthening the Ayurveda ecosystem."],"authorString":["Patwardhan B."],"authorList":[{"author":[{"fullName":["Patwardhan B"],"firstName":["Bhushan"],"lastName":["Patwardhan"],"initials":["B"],"affiliation":["Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, Pune, Maharashtra, India. Electronic address: bpatwardhan@gmail.com."]}]}],"journalInfo":[{"issue":["2"],"volume":["7"],"journalIssueId":["2435826"],"dateOfPublication":["2016 Apr-Jun"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-04-01"],"journal":[{"title":["Journal of Ayurveda and integrative medicine"],"ISOAbbreviation":["J Ayurveda Integr Med"],"medlineAbbreviation":["J Ayurveda Integr Med"],"NLMid":["101551404"],"ISSN":["0975-9476"],"ESSN":["0976-2809"]}]}],"pubYear":["2016"],"pageInfo":["73-75"],"affiliation":["Interdisciplinary School of Health Sciences, Savitribai Phule Pune University, Pune, Maharashtra, India. Electronic address: bpatwardhan@gmail.com."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Editorial"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4969310"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1016/j.jaim.2016.07.002"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["N"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc-nd"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-08-02"],"dateOfCreation":["2016-08-02"],"dateOfRevision":["2016-08-09"],"electronicPublicationDate":["2016-07-21"],"firstPublicationDate":["2016-07-20"],"cprojectID":"PMC4969310"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrx6_8VqgcuJwvjo","_score":1,"_source":{"id":["27429555"],"source":["MED"],"pmid":["27429555"],"pmcid":["PMC4941868"],"doi":["10.4137/ehi.s39805"],"title":["A Performance Management Initiative for Local Health Department Vector Control Programs."],"authorString":["Gerding J, Kirshy M, Moran JW, Bialek R, Lamers V, Sarisky J."],"authorList":[{"author":[{"fullName":["Gerding J"],"firstName":["Justin"],"lastName":["Gerding"],"initials":["J"],"affiliation":["Centers for Disease Control and Prevention, Atlanta, GA, USA."]},{"fullName":["Kirshy M"],"firstName":["Micaela"],"lastName":["Kirshy"],"initials":["M"],"affiliation":["Public Health Foundation, Washington, DC, USA."]},{"fullName":["Moran JW"],"firstName":["John W"],"lastName":["Moran"],"initials":["JW"],"affiliation":["Public Health Foundation, Washington, DC, USA."]},{"fullName":["Bialek R"],"firstName":["Ron"],"lastName":["Bialek"],"initials":["R"],"affiliation":["Public Health Foundation, Washington, DC, USA."]},{"fullName":["Lamers V"],"firstName":["Vanessa"],"lastName":["Lamers"],"initials":["V"],"affiliation":["Public Health Foundation, Washington, DC, USA."]},{"fullName":["Sarisky J"],"firstName":["John"],"lastName":["Sarisky"],"initials":["J"],"affiliation":["Centers for Disease Control and Prevention, Atlanta, GA, USA."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2400530"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Environmental health insights"],"ISOAbbreviation":["Environ Health Insights"],"medlineAbbreviation":["Environ Health Insights"],"NLMid":["101488505"],"ESSN":["1178-6302"]}]}],"pubYear":["2016"],"pageInfo":["113-118"],"abstractText":["Local health department (LHD) vector control programs have experienced reductions in funding and capacity. Acknowledging this situation and its potential effect on the ability to respond to vector-borne diseases, the U.S. Centers for Disease Control and Prevention and the Public Health Foundation partnered on a performance management initiative for LHD vector control programs. The initiative involved 14 programs that conducted a performance assessment using the Environmental Public Health Performance Standards. The programs, assisted by quality improvement (QI) experts, used the assessment results to prioritize improvement areas that were addressed with QI projects intended to increase effectiveness and efficiency in the delivery of services such as responding to mosquito complaints and educating the public about vector-borne disease prevention. This article describes the initiative as a process LHD vector control programs may adapt to meet their performance management needs. This study also reviews aggregate performance assessment results and QI projects, which may reveal common aspects of LHD vector control program performance and priority improvement areas. LHD vector control programs interested in performance assessment and improvement may benefit from engaging in an approach similar to this performance management initiative."],"affiliation":["Centers for Disease Control and Prevention, Atlanta, GA, USA."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Quality improvement","Mosquito control","Environmental Health","Performance Management","Performance Standards","Vector Control","Performance Assessment"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4941868?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4941868"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.4137/EHI.S39805"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-07-19"],"dateOfCreation":["2016-07-19"],"dateOfRevision":["2016-07-20"],"electronicPublicationDate":["2016-07-11"],"firstPublicationDate":["2016-07-11"],"cprojectID":"PMC4941868"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzMJ9hGurOzZIbv","_score":1,"_source":{"id":["27487767"],"source":["MED"],"pmid":["27487767"],"pmcid":["PMC4973112"],"doi":["10.1186/s12916-016-0660-0"],"title":["The global threat of Zika virus to pregnancy: epidemiology, clinical perspectives, mechanisms, and impact."],"authorString":["Boeuf P, Drummer HE, Richards JS, Scoullar MJ, Beeson JG."],"authorList":[{"author":[{"fullName":["Boeuf P"],"firstName":["Phillipe"],"lastName":["Boeuf"],"initials":["P"],"affiliation":["Department of Medicine, The University of Melbourne, Melbourne, Australia. philippe.boeuf@burnet.edu.au."]},{"fullName":["Drummer HE"],"firstName":["Heidi E"],"lastName":["Drummer"],"initials":["HE"],"affiliation":["Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Australia."]},{"fullName":["Richards JS"],"firstName":["Jack S"],"lastName":["Richards"],"initials":["JS"],"affiliation":["Department of Microbiology, Monash University, Clayton, Australia."]},{"fullName":["Scoullar MJ"],"firstName":["Michelle J L"],"lastName":["Scoullar"],"initials":["MJ"],"affiliation":["Department of Medicine, The University of Melbourne, Melbourne, Australia."]},{"fullName":["Beeson JG"],"firstName":["James G"],"lastName":["Beeson"],"initials":["JG"],"affiliation":["Department of Microbiology, Monash University, Clayton, Australia. beeson@burnet.edu.au."]}]}],"journalInfo":[{"issue":["1"],"volume":["14"],"journalIssueId":["2358711"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC medicine"],"ISOAbbreviation":["BMC Med"],"medlineAbbreviation":["BMC Med"],"NLMid":["101190723"],"ESSN":["1741-7015"]}]}],"pubYear":["2016"],"pageInfo":["112"],"abstractText":["Zika virus (ZIKV) is a mosquito-borne flavivirus that has newly emerged as a significant global threat, especially to pregnancy. Recent major outbreaks in the Pacific and in Central and South America have been associated with an increased incidence of microcephaly and other abnormalities of the central nervous system in neonates. The causal link between ZIKV infection during pregnancy and microcephaly is now strongly supported. Over 2 billion people live in regions conducive to ZIKV transmission, with ~4 million infections in the Americas predicted for 2016. Given the scale of the current pandemic and the serious and long-term consequences of infection during pregnancy, the impact of ZIKV on health services and affected communities could be enormous. This further highlights the need for a rapid global public health and research response to ZIKV to limit and prevent its impact through the development of therapeutics, vaccines, and improved diagnostics. Here we review the epidemiology of ZIKV; the threat to pregnancy; the clinical consequences and broader impact of ZIKV infections; and the virus biology underpinning new interventions, diagnostics, and insights into the mechanisms of disease."],"affiliation":["Department of Medicine, The University of Melbourne, Melbourne, Australia. philippe.boeuf@burnet.edu.au."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Biology","Placenta","Pregnancy","Pathogenesis","epidemiology","Public Health","Microcephaly","Economic Cost"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973112?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4973112"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12916-016-0660-0"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-04"],"dateOfRevision":["2016-08-07"],"electronicPublicationDate":["2016-08-03"],"firstPublicationDate":["2016-08-03"],"cprojectID":"PMC4973112"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnrzO_8VqgcuJwvjt","_score":1,"_source":{"id":["27443488"],"source":["MED"],"pmid":["27443488"],"pmcid":["PMC4956753"],"doi":["10.1038/srep30016"],"title":["Mitonuclear interactions, mtDNA-mediated thermal plasticity, and implications for the Trojan Female Technique for pest control."],"authorString":["Wolff JN, Tompkins DM, Gemmell NJ, Dowling DK."],"authorList":[{"author":[{"fullName":["Wolff JN"],"firstName":["Jonci N"],"lastName":["Wolff"],"initials":["JN"],"affiliation":["School of Biological Sciences, Monash University, Victoria, 3800, Australia."]},{"fullName":["Tompkins DM"],"firstName":["Daniel M"],"lastName":["Tompkins"],"initials":["DM"],"affiliation":["Landcare Research, Private Bag 1930, Dunedin, New Zealand."]},{"fullName":["Gemmell NJ"],"firstName":["Neil J"],"lastName":["Gemmell"],"initials":["NJ"],"affiliation":["Allan Wilson Centre for Molecular Ecology and Evolution, Department of Anatomy, University of Otago, Dunedin 9016, New Zealand."]},{"fullName":["Dowling DK"],"firstName":["Damian K"],"lastName":["Dowling"],"initials":["DK"],"affiliation":["School of Biological Sciences, Monash University, Victoria, 3800, Australia."]}]}],"journalInfo":[{"volume":["6"],"journalIssueId":["2358449"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Scientific reports"],"ISOAbbreviation":["Sci Rep"],"medlineAbbreviation":["Sci Rep"],"NLMid":["101563288"],"ESSN":["2045-2322"]}]}],"pubYear":["2016"],"pageInfo":["30016"],"abstractText":["Pest species pose major challenges to global economies, ecosystems, and health. Unfortunately, most conventional approaches to pest control remain costly, and temporary in effect. As such, a heritable variant of the Sterile Insect Technique (SIT) was proposed, based on the introduction of mitochondrial DNA mutations into pest populations, which impair male fertility but have no effects on females. Evidence for this \"Trojan Female Technique\" (TFT) was recently provided, in the form of a mutation in the mitochondrial cytochrome b gene (mt:Cyt-b) of Drosophila melanogaster which reduces male fertility across diverse nuclear backgrounds. However, recent studies have shown that the magnitude of mitochondrial genetic effects on the phenotype can vary greatly across environments, with mtDNA polymorphisms commonly entwined in genotype-by-environment (G × E) interactions. Here we test whether the male-sterilizing effects previously associated with the mt:Cyt-b mutation are consistent across three thermal and three nuclear genomic contexts. The effects of this mutation were indeed moderated by the nuclear background and thermal environment, but crucially the fertility of males carrying the mutation was invariably reduced relative to controls. This mutation thus constitutes a promising candidate for the further development of the TFT."],"affiliation":["School of Biological Sciences, Monash University, Victoria, 3800, Australia."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4956753?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4956753"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1038/srep30016"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-07-22"],"dateOfRevision":["2016-07-27"],"electronicPublicationDate":["2016-07-21"],"firstPublicationDate":["2016-07-21"],"cprojectID":"PMC4956753"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr2y_8VqgcuJwvj5","_score":1,"_source":{"id":["27493451"],"source":["MED"],"pmid":["27493451"],"pmcid":["PMC4963559"],"doi":["10.1155/2016/4121837"],"title":["Inflammation Thread Runs across Medical Laboratory Specialities."],"authorString":["Nydegger U, Lung T, Risch L, Risch M, Medina Escobar P, Bodmer T."],"authorList":[{"author":[{"fullName":["Nydegger U"],"firstName":["Urs"],"lastName":["Nydegger"],"initials":["U"],"authorId":[{"_":"0000-0002-2584-5873","$":{"type":"ORCID"}}],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]},{"fullName":["Lung T"],"firstName":["Thomas"],"lastName":["Lung"],"initials":["T"],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]},{"fullName":["Risch L"],"firstName":["Lorenz"],"lastName":["Risch"],"initials":["L"],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]},{"fullName":["Risch M"],"firstName":["Martin"],"lastName":["Risch"],"initials":["M"],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]},{"fullName":["Medina Escobar P"],"firstName":["Pedro"],"lastName":["Medina Escobar"],"initials":["P"],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]},{"fullName":["Bodmer T"],"firstName":["Thomas"],"lastName":["Bodmer"],"initials":["T"],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-2584-5873","$":{"type":"ORCID"}}]}],"journalInfo":[{"volume":["2016"],"journalIssueId":["2389471"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Mediators of inflammation"],"ISOAbbreviation":["Mediators Inflamm."],"medlineAbbreviation":["Mediators Inflamm"],"NLMid":["9209001"],"ISSN":["0962-9351"],"ESSN":["1466-1861"]}]}],"pubYear":["2016"],"pageInfo":["4121837"],"abstractText":["We work on the assumption that four major specialities or sectors of medical laboratory assays, comprising clinical chemistry, haematology, immunology, and microbiology, embraced by genome sequencing techniques, are routinely in use. Medical laboratory markers for inflammation serve as model: they are allotted to most fields of medical lab assays including genomics. Incessant coding of assays aligns each of them in the long lists of big data. As exemplified with the complement gene family, containing C2, C3, C8A, C8B, CFH, CFI, and ITGB2, heritability patterns/risk factors associated with diseases with genetic glitch of complement components are unfolding. The C4 component serum levels depend on sufficient vitamin D whilst low vitamin D is inversely related to IgG1, IgA, and C3 linking vitamin sufficiency to innate immunity. Whole genome sequencing of microbial organisms may distinguish virulent from nonvirulent and antibiotic resistant from nonresistant varieties of the same species and thus can be listed in personal big data banks including microbiological pathology; the big data warehouse continues to grow."],"affiliation":["Labormedizinisches Zentrum Dr. Risch and Kantonsspital Graubünden, 7000 Chur, Switzerland."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963559?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4963559"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1155/2016/4121837"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-08-05"],"dateOfRevision":["2016-08-08"],"electronicPublicationDate":["2016-07-14"],"firstPublicationDate":["2016-07-14"],"cprojectID":"PMC4963559"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnr34J9hGurOzZIb5","_score":1,"_source":{"id":["27555815"],"source":["MED"],"pmid":["27555815"],"pmcid":["PMC4977292"],"doi":["10.3389/fnhum.2016.00403"],"title":["A Possible Mechanism of Zika Virus Associated Microcephaly: Imperative Role of Retinoic Acid Response Element (RARE) Consensus Sequence Repeats in the Viral Genome."],"authorString":["Kumar A, Singh HN, Pareek V, Raza K, Dantham S, Kumar P, Mochan S, Faiq MA."],"authorList":[{"author":[{"fullName":["Kumar A"],"firstName":["Ashutosh"],"lastName":["Kumar"],"initials":["A"],"affiliation":["Department of Anatomy, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Singh HN"],"firstName":["Himanshu N"],"lastName":["Singh"],"initials":["HN"],"affiliation":["Department of Biochemistry, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Pareek V"],"firstName":["Vikas"],"lastName":["Pareek"],"initials":["V"],"affiliation":["Computational Neuroscience and Neuroimaging Division, National Brain Research Centre Manesar, India."]},{"fullName":["Raza K"],"firstName":["Khursheed"],"lastName":["Raza"],"initials":["K"],"affiliation":["Department of Anatomy, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Dantham S"],"firstName":["Subrahamanyam"],"lastName":["Dantham"],"initials":["S"],"affiliation":["Department of Biochemistry, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Kumar P"],"firstName":["Pavan"],"lastName":["Kumar"],"initials":["P"],"affiliation":["Department of Anatomy, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Mochan S"],"firstName":["Sankat"],"lastName":["Mochan"],"initials":["S"],"affiliation":["Department of Anatomy, All India Institute of Medical Sciences New Delhi, India."]},{"fullName":["Faiq MA"],"firstName":["Muneeb A"],"lastName":["Faiq"],"initials":["MA"],"affiliation":["Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical SciencesNew Delhi, India; Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of medical SciencesNew Delhi, India; Medical Biotechnology Laboratory, Dr. B. R. Ambedkar Centre for Biomedical Research, University of DelhiNew Delhi, India."]}]}],"journalInfo":[{"volume":["10"],"journalIssueId":["2390596"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Frontiers in human neuroscience"],"ISOAbbreviation":["Front Hum Neurosci"],"medlineAbbreviation":["Front Hum Neurosci"],"NLMid":["101477954"],"ESSN":["1662-5161"]}]}],"pubYear":["2016"],"pageInfo":["403"],"abstractText":["Owing to the reports of microcephaly as a consistent outcome in the fetuses of pregnant women infected with ZIKV in Brazil, Zika virus (ZIKV)-microcephaly etiomechanistic relationship has recently been implicated. Researchers, however, are still struggling to establish an embryological basis for this interesting causal handcuff. The present study reveals robust evidence in favor of a plausible ZIKV-microcephaly cause-effect liaison. The rationale is based on: (1) sequence homology between ZIKV genome and the response element of an early neural tube developmental marker \"retinoic acid\" in human DNA and (2) comprehensive similarities between the details of brain defects in ZIKV-microcephaly and retinoic acid embryopathy. Retinoic acid is considered as the earliest factor for regulating anteroposterior axis of neural tube and positioning of structures in developing brain through retinoic acid response elements (RARE) consensus sequence (5'-AGGTCA-3') in promoter regions of retinoic acid-dependent genes. We screened genomic sequences of already reported virulent ZIKV strains (including those linked to microcephaly) and other viruses available in National Institute of Health genetic sequence database (GenBank) for the RARE consensus repeats and obtained results strongly bolstering our hypothesis that ZIKV strains associated with microcephaly may act through precipitation of dysregulation in retinoic acid-dependent genes by introducing extra stretches of RARE consensus sequence repeats in the genome of developing brain cells. Additional support to our hypothesis comes from our findings that screening of other viruses for RARE consensus sequence repeats is positive only for those known to display neurotropism and cause fetal brain defects (for which maternal-fetal transmission during developing stage may be required). The numbers of RARE sequence repeats appeared to match with the virulence of screened positive viruses. Although, bioinformatic evidence and embryological features are in favor of our hypothesis, additional studies including animal models are warranted to validate our proposition. Such studies are likely to unfold ZIKV-microcephaly association and may help in devising methods to combat it."],"affiliation":["Department of Anatomy, All India Institute of Medical Sciences New Delhi, India."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["neurotropism","Microcephaly","Zika Virus (Zikv)","Fetal Brain Malformation","Retinoic Acid Response Element (Rare)"]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4977292?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4977292"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3389/fnhum.2016.00403"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," refseq"," "]}],"dateOfCompletion":["2016-08-24"],"dateOfCreation":["2016-08-24"],"dateOfRevision":["2016-08-26"],"electronicPublicationDate":["2016-08-09"],"firstPublicationDate":["2016-08-09"],"cprojectID":"PMC4977292"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqoJhOUS2orL1IOe","_score":1,"_source":{"id":["25719412"],"source":["MED"],"pmid":["25719412"],"pmcid":["PMC4342338"],"doi":["10.1371/journal.pone.0117849"],"title":["New insights into flavivirus evolution, taxonomy and biogeographic history, extended by analysis of canonical and alternative coding sequences."],"authorString":["Moureau G, Cook S, Lemey P, Nougairede A, Forrester NL, Khasnatinov M, Charrel RN, Firth AE, Gould EA, de Lamballerie X."],"authorList":[{"author":[{"fullName":["Moureau G"],"firstName":["Gregory"],"lastName":["Moureau"],"initials":["G"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."]},{"fullName":["Cook S"],"firstName":["Shelley"],"lastName":["Cook"],"initials":["S"],"affiliation":["Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, United Kingdom."]},{"fullName":["Lemey P"],"firstName":["Philippe"],"lastName":["Lemey"],"initials":["P"],"affiliation":["Department of Microbiology and Immunology, Rega Institute, KU Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium."]},{"fullName":["Nougairede A"],"firstName":["Antoine"],"lastName":["Nougairede"],"initials":["A"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."]},{"fullName":["Forrester NL"],"firstName":["Naomi L"],"lastName":["Forrester"],"initials":["NL"],"affiliation":["Institute for Human Infections and Immunity and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, United States of America."]},{"fullName":["Khasnatinov M"],"firstName":["Maxim"],"lastName":["Khasnatinov"],"initials":["M"],"affiliation":["Centre for Ecology and Hydrology, Maclean Building, Benson Lane, Crowmarsh, Gifford, Wallingford, Oxfordshire, OX10, United Kingdom."]},{"fullName":["Charrel RN"],"firstName":["Remi N"],"lastName":["Charrel"],"initials":["RN"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."]},{"fullName":["Firth AE"],"firstName":["Andrew E"],"lastName":["Firth"],"initials":["AE"],"affiliation":["Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom."]},{"fullName":["Gould EA"],"firstName":["Ernest A"],"lastName":["Gould"],"initials":["EA"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."]},{"fullName":["de Lamballerie X"],"firstName":["Xavier"],"lastName":["de Lamballerie"],"initials":["X"],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."]}]}],"journalInfo":[{"issue":["2"],"volume":["10"],"journalIssueId":["2248873"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0117849"],"abstractText":["To generate the most diverse phylogenetic dataset for the flaviviruses to date, we determined the genomic sequences and phylogenetic relationships of 14 flaviviruses, of which 10 are primarily associated with Culex spp. mosquitoes. We analyze these data, in conjunction with a comprehensive collection of flavivirus genomes, to characterize flavivirus evolutionary and biogeographic history in unprecedented detail and breadth. Based on the presumed introduction of yellow fever virus into the Americas via the transatlantic slave trade, we extrapolated a timescale for a relevant subset of flaviviruses whose evolutionary history, shows that different Culex-spp. associated flaviviruses have been introduced from the Old World to the New World on at least five separate occasions, with 2 different sets of factors likely to have contributed to the dispersal of the different viruses. We also discuss the significance of programmed ribosomal frameshifting in a central region of the polyprotein open reading frame in some mosquito-associated flaviviruses."],"affiliation":["Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, Marseille, France."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"grantsList":[{"grant":[{"grantId":["088789"],"agency":["Wellcome Trust"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Flavivirus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["N"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["Y"]},{"abbreviation":["PH"],"qualifierName":["physiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Evolution, Molecular"]},{"majorTopic_YN":["Y"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Frameshifting, Ribosomal"]},{"majorTopic_YN":["N"],"descriptorName":["Base Sequence"]},{"majorTopic_YN":["Y"],"descriptorName":["Genome, Viral"]},{"majorTopic_YN":["Y"],"descriptorName":["Open Reading Frames"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Data"]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeography"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4342338?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4342338"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0117849"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["3"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," refseq"," "]}],"dateOfCompletion":["2016-01-18"],"dateOfCreation":["2015-02-27"],"dateOfRevision":["2015-03-05"],"electronicPublicationDate":["2015-02-26"],"firstPublicationDate":["2015-02-26"],"cprojectID":"PMC4342338"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqsD_8VqgcuJwvga","_score":1,"_source":{"id":["25530324"],"source":["MED"],"pmid":["25530324"],"pmcid":["PMC4285245"],"doi":["10.3201/eid2101.140894"],"title":["Detection of Zika virus in urine."],"authorString":["Gourinat AC, O'Connor O, Calvez E, Goarant C, Dupont-Rouzeyrol M."],"authorList":[{"author":[{"fullName":["Gourinat AC"],"firstName":["Ann-Claire"],"lastName":["Gourinat"],"initials":["AC"]},{"fullName":["O'Connor O"],"firstName":["Olivia"],"lastName":["O'Connor"],"initials":["O"]},{"fullName":["Calvez E"],"firstName":["Elodie"],"lastName":["Calvez"],"initials":["E"]},{"fullName":["Goarant C"],"firstName":["Cyrille"],"lastName":["Goarant"],"initials":["C"],"authorId":[{"_":"0000-0002-0690-9464","$":{"type":"ORCID"}}]},{"fullName":["Dupont-Rouzeyrol M"],"firstName":["Myrielle"],"lastName":["Dupont-Rouzeyrol"],"initials":["M"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-0690-9464","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["21"],"journalIssueId":["2233313"],"dateOfPublication":["2015 Jan"],"monthOfPublication":["1"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2015"],"pageInfo":["84-86"],"abstractText":["We describe the kinetics of Zika virus (ZIKV) detection in serum and urine samples of 6 patients. Urine samples were positive for ZIKV >10 days after onset of disease, which was a notably longer period than for serum samples. This finding supports the conclusion that urine samples are useful for diagnosis of ZIKV infections."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["UR"],"qualifierName":["urine"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Molecular Diagnostic Techniques"]},{"majorTopic_YN":["N"],"descriptorName":["Reverse Transcriptase Polymerase Chain Reaction"]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["UR"],"qualifierName":["urine"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4285245?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4285245"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2101.140894"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["20"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"]}],"dateOfCompletion":["2015-09-10"],"dateOfCreation":["2014-12-22"],"dateOfRevision":["2016-02-03"],"firstPublicationDate":["2015-01-01"],"cprojectID":"PMC4285245"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqtehOUS2orL1IOt","_score":1,"_source":{"id":["26200711"],"source":["MED"],"pmid":["26200711"],"pmcid":["PMC4569826"],"doi":["10.1590/0074-02760150103"],"title":["Biocontrol evaluation of extracts and a major component, clusianone, from Clusia fluminensis Planch. & Triana against Aedes aegypti."],"authorString":["Anholeti MC, Duprat RC, Figueiredo MR, Kaplan MA, Santos MG, Gonzalez MS, Ratcliffe NA, Feder D, Paiva SR, Mello CB."],"authorList":[{"author":[{"fullName":["Anholeti MC"],"firstName":["Maria C"],"lastName":["Anholeti"],"initials":["MC"],"affiliation":["Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR."]},{"fullName":["Duprat RC"],"firstName":["Rodrigo C"],"lastName":["Duprat"],"initials":["RC"],"affiliation":["Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niterói, RJ, BR."]},{"fullName":["Figueiredo MR"],"firstName":["Maria R"],"lastName":["Figueiredo"],"initials":["MR"],"affiliation":["Laboratório de Química de Produtos Naturais, Farmanguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BR."]},{"fullName":["Kaplan MA"],"firstName":["Maria Ac"],"lastName":["Kaplan"],"initials":["MA"],"affiliation":["nstituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR."]},{"fullName":["Santos MG"],"firstName":["Marcelo Guerra"],"lastName":["Santos"],"initials":["MG"],"affiliation":["Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, BR."]},{"fullName":["Gonzalez MS"],"firstName":["Marcelo S"],"lastName":["Gonzalez"],"initials":["MS"],"affiliation":["Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niterói, RJ, BR."]},{"fullName":["Ratcliffe NA"],"firstName":["Norman A"],"lastName":["Ratcliffe"],"initials":["NA"],"affiliation":["Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niterói, RJ, BR."]},{"fullName":["Feder D"],"firstName":["Denise"],"lastName":["Feder"],"initials":["D"],"affiliation":["Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niterói, RJ, BR."]},{"fullName":["Paiva SR"],"firstName":["Selma R"],"lastName":["Paiva"],"initials":["SR"],"affiliation":["Laboratório de Botânica Estrutural e Funcional, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, BR."]},{"fullName":["Mello CB"],"firstName":["Cicero B"],"lastName":["Mello"],"initials":["CB"],"affiliation":["Laboratório de Biologia de Insetos, Universidade Federal Fluminense, Niterói, RJ, BR."]}]}],"journalInfo":[{"issue":["5"],"volume":["110"],"journalIssueId":["2317435"],"dateOfPublication":["2015 Aug"],"monthOfPublication":["8"],"yearOfPublication":["2015"],"printPublicationDate":["2015-08-01"],"journal":[{"title":["Memorias do Instituto Oswaldo Cruz"],"ISOAbbreviation":["Mem. Inst. Oswaldo Cruz"],"medlineAbbreviation":["Mem Inst Oswaldo Cruz"],"NLMid":["7502619"],"ISSN":["0074-0276"],"ESSN":["1678-8060"]}]}],"pubYear":["2015"],"pageInfo":["629-635"],"abstractText":["Studies evaluated the effects of hexanic extracts from the fruits and flowers of Clusia fluminensis and the main component of the flower extract, a purified benzophenone (clusianone), against Aedes aegypti. The treatment of larvae with the crude fruit or flower extracts from C. fluminensis did not affect the survival ofAe. aegypti (50 mg/L), however, the flower extracts significantly delayed development of Ae. aegypti. In contrast, the clusianone (50 mg/L) isolate from the flower extract, representing 54.85% of this sample composition, showed a highly significant inhibition of survival, killing 93.3% of the larvae and completely blocking development of Ae. aegypti. The results showed, for the first time, high activity of clusianone against Ae. aegypti that both killed and inhibited mosquito development. Therefore, clusianone has potential for development as a biopesticide for controlling insect vectors of tropical diseases. Future work will elucidate the mode of action of clusianone isolated from C. fluminensis."],"affiliation":["Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BR."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Aedes"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Clusia"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CH"],"qualifierName":["chemistry"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Plant Extracts"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PD"],"qualifierName":["pharmacology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Insect Vectors"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Larva"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DE"],"qualifierName":["drug effects"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Time Factors"]}]}],"chemicalList":[{"chemical":[{"name":["Plant Extracts"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4569826?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4569826"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1590/0074-02760150103"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["1"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"license":["cc by-nc"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-02-22"],"dateOfCreation":["2015-08-28"],"dateOfRevision":["2015-09-16"],"electronicPublicationDate":["2015-07-17"],"firstPublicationDate":["2015-07-17"],"cprojectID":"PMC4569826"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnquHJ9hGurOzZIYf","_score":1,"_source":{"id":["26372010"],"source":["MED"],"pmid":["26372010"],"pmcid":["PMC4570817"],"doi":["10.1371/journal.pntd.0004073"],"title":["Syndromic Approach to Arboviral Diagnostics for Global Travelers as a Basis for Infectious Disease Surveillance."],"authorString":["Cleton NB, Reusken CB, Wagenaar JF, van der Vaart EE, Reimerink J, van der Eijk AA, Koopmans MP."],"authorList":[{"author":[{"fullName":["Cleton NB"],"firstName":["Natalie B"],"lastName":["Cleton"],"initials":["NB"],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands; National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands."]},{"fullName":["Reusken CB"],"firstName":["Chantal B E M"],"lastName":["Reusken"],"initials":["CB"],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands."]},{"fullName":["Wagenaar JF"],"firstName":["Jiri F P"],"lastName":["Wagenaar"],"initials":["JF"],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands."]},{"fullName":["van der Vaart EE"],"firstName":["Elske E"],"lastName":["van der Vaart"],"initials":["EE"],"affiliation":["University of Reading, Reading, Berkshire, United Kingdom."]},{"fullName":["Reimerink J"],"firstName":["Johan"],"lastName":["Reimerink"],"initials":["J"],"affiliation":["National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands."]},{"fullName":["van der Eijk AA"],"firstName":["Annemiek A"],"lastName":["van der Eijk"],"initials":["AA"],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands."]},{"fullName":["Koopmans MP"],"firstName":["Marion P G"],"lastName":["Koopmans"],"initials":["MP"],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands; National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands."]}]}],"journalInfo":[{"issue":["9"],"volume":["9"],"journalIssueId":["2371199"],"dateOfPublication":["2015 "],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PLoS neglected tropical diseases"],"ISOAbbreviation":["PLoS Negl Trop Dis"],"medlineAbbreviation":["PLoS Negl Trop Dis"],"NLMid":["101291488"],"ISSN":["1935-2727"],"ESSN":["1935-2735"]}]}],"pubYear":["2015"],"pageInfo":["e0004073"],"abstractText":["Arboviruses have overlapping geographical distributions and can cause symptoms that coincide with more common infections. Therefore, arbovirus infections are often neglected by travel diagnostics. Here, we assessed the potential of syndrome-based approaches for diagnosis and surveillance of neglected arboviral diseases in returning travelers.To map the patients high at risk of missed clinical arboviral infections we compared the quantity of all arboviral diagnostic requests by physicians in the Netherlands, from 2009 through 2013, with a literature-based assessment of the travelers' likely exposure to an arbovirus.2153 patients, with travel and clinical history were evaluated. The diagnostic assay for dengue virus (DENV) was the most commonly requested (86%). Of travelers returning from Southeast Asia with symptoms compatible with chikungunya virus (CHIKV), only 55% were tested. For travelers in Europe, arbovirus diagnostics were rarely requested. Over all, diagnostics for most arboviruses were requested only on severe clinical presentation.Travel destination and syndrome were used inconsistently for triage of diagnostics, likely resulting in vast under-diagnosis of arboviral infections of public health significance. This study shows the need for more awareness among physicians and standardization of syndromic diagnostic algorithms."],"affiliation":["Erasmus Medical Centre, Rotterdam, The Netherlands; National Institute for Public Health and Environment (RIVM), Bilthoven, The Netherlands."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Arbovirus Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["Y"]},{"abbreviation":["EP"],"qualifierName":["epidemiology"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Travel"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["Y"],"descriptorName":["Epidemiological Monitoring"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4570817?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4570817"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pntd.0004073"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-03-28"],"dateOfCreation":["2015-09-16"],"dateOfRevision":["2015-09-19"],"electronicPublicationDate":["2015-09-15"],"firstPublicationDate":["2015-09-15"],"cprojectID":"PMC4570817"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqx0_8VqgcuJwvgq","_score":1,"_source":{"id":["26020623"],"source":["MED"],"pmid":["26020623"],"pmcid":["PMC4447349"],"doi":["10.1371/journal.pone.0127465"],"title":["Maternal Diabetes Leads to Adaptation in Embryonic Amino Acid Metabolism during Early Pregnancy."],"authorString":["Gürke J, Hirche F, Thieme R, Haucke E, Schindler M, Stangl GI, Fischer B, Navarrete Santos A."],"authorList":[{"author":[{"fullName":["Gürke J"],"firstName":["Jacqueline"],"lastName":["Gürke"],"initials":["J"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]},{"fullName":["Hirche F"],"firstName":["Frank"],"lastName":["Hirche"],"initials":["F"],"affiliation":["Department of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany."]},{"fullName":["Thieme R"],"firstName":["René"],"lastName":["Thieme"],"initials":["R"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]},{"fullName":["Haucke E"],"firstName":["Elisa"],"lastName":["Haucke"],"initials":["E"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]},{"fullName":["Schindler M"],"firstName":["Maria"],"lastName":["Schindler"],"initials":["M"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]},{"fullName":["Stangl GI"],"firstName":["Gabriele I"],"lastName":["Stangl"],"initials":["GI"],"affiliation":["Department of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany."]},{"fullName":["Fischer B"],"firstName":["Bernd"],"lastName":["Fischer"],"initials":["B"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]},{"fullName":["Navarrete Santos A"],"firstName":["Anne"],"lastName":["Navarrete Santos"],"initials":["A"],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."]}]}],"journalInfo":[{"issue":["5"],"volume":["10"],"journalIssueId":["2278532"],"dateOfPublication":["2015"],"monthOfPublication":["0"],"yearOfPublication":["2015"],"printPublicationDate":["2015-01-01"],"journal":[{"title":["PloS one"],"ISOAbbreviation":["PLoS ONE"],"medlineAbbreviation":["PLoS One"],"NLMid":["101285081"],"ESSN":["1932-6203"]}]}],"pubYear":["2015"],"pageInfo":["e0127465"],"abstractText":["During pregnancy an adequate amino acid supply is essential for embryo development and fetal growth. We have studied amino acid composition and branched chain amino acid (BCAA) metabolism at day 6 p.c. in diabetic rabbits and blastocysts. In the plasma of diabetic rabbits the concentrations of 12 amino acids were altered in comparison to the controls. Notably, the concentrations of the BCAA leucine, isoleucine and valine were approximately three-fold higher in diabetic rabbits than in the control. In the cavity fluid of blastocysts from diabetic rabbits BCAA concentrations were twice as high as those from controls, indicating a close link between maternal diabetes and embryonic BCAA metabolism. The expression of BCAA oxidizing enzymes and BCAA transporter was analysed in maternal tissues and in blastocysts. The RNA amounts of three oxidizing enzymes, i.e. branched chain aminotransferase 2 (Bcat2), branched chain ketoacid dehydrogenase (Bckdha) and dehydrolipoyl dehydrogenase (Dld), were markedly increased in maternal adipose tissue and decreased in liver and skeletal muscle of diabetic rabbits than in those of controls. Blastocysts of diabetic rabbits revealed a higher Bcat2 mRNA and protein abundance in comparison to control blastocysts. The expression of BCAA transporter LAT1 and LAT2 were unaltered in endometrium of diabetic and healthy rabbits, whereas LAT2 transcripts were increased in blastocysts of diabetic rabbits. In correlation to high embryonic BCAA levels the phosphorylation amount of the nutrient sensor mammalian target of rapamycin (mTOR) was enhanced in blastocysts caused by maternal diabetes. These results demonstrate a direct impact of maternal diabetes on BCAA concentrations and degradation in mammalian blastocysts with influence on embryonic mTOR signalling."],"affiliation":["Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle (Saale), Germany."],"language":["eng"],"pubModel":["Electronic-eCollection"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Rabbits"]},{"majorTopic_YN":["N"],"descriptorName":["Pregnancy Complications"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Diabetes Mellitus, Experimental"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Amino Acids, Branched-Chain"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Large Neutral Amino Acid-Transporter 1"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["Y"],"descriptorName":["Adaptation, Physiological"]},{"majorTopic_YN":["Y"],"descriptorName":["Signal Transduction"]},{"majorTopic_YN":["N"],"descriptorName":["Pregnancy"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Embryo, Mammalian"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["Y"]},{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["TOR Serine-Threonine Kinases"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ME"],"qualifierName":["metabolism"],"majorTopic_YN":["N"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["Amino Acids, Branched-Chain"],"registryNumber":["0"]},{"name":["Large Neutral Amino Acid-Transporter 1"],"registryNumber":["0"]},{"name":["TOR Serine-Threonine Kinases"],"registryNumber":["EC 2.7.1.1"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4447349?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4447349"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1371/journal.pone.0127465"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2016-04-01"],"dateOfCreation":["2015-05-29"],"dateOfRevision":["2015-06-10"],"electronicPublicationDate":["2015-05-28"],"firstPublicationDate":["2015-05-28"],"cprojectID":"PMC4447349"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnq3uJ9hGurOzZIY0","_score":1,"_source":{"id":["26838717"],"source":["MED"],"pmid":["26838717"],"pmcid":["PMC4742707"],"doi":["10.1128/mbio.01938-15"],"title":["Reconstruction of the Evolutionary History and Dispersal of Usutu Virus, a Neglected Emerging Arbovirus in Europe and Africa."],"authorString":["Engel D, Jöst H, Wink M, Börstler J, Bosch S, Garigliany MM, Jöst A, Czajka C, Lühken R, Ziegler U, Groschup MH, Pfeffer M, Becker N, Cadar D, Schmidt-Chanasit J."],"authorList":[{"author":[{"fullName":["Engel D"],"firstName":["Dimitri"],"lastName":["Engel"],"initials":["D"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany."]},{"fullName":["Jöst H"],"firstName":["Hanna"],"lastName":["Jöst"],"initials":["H"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany."]},{"fullName":["Wink M"],"firstName":["Michael"],"lastName":["Wink"],"initials":["M"],"affiliation":["Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany."]},{"fullName":["Börstler J"],"firstName":["Jessica"],"lastName":["Börstler"],"initials":["J"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany."]},{"fullName":["Bosch S"],"firstName":["Stefan"],"lastName":["Bosch"],"initials":["S"],"affiliation":["Nature and Biodiversity Conservation Union (NABU), Stuttgart, Germany."]},{"fullName":["Garigliany MM"],"firstName":["Mutien-Marie"],"lastName":["Garigliany"],"initials":["MM"],"affiliation":["Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium."]},{"fullName":["Jöst A"],"firstName":["Artur"],"lastName":["Jöst"],"initials":["A"],"affiliation":["German Mosquito Control Association (KABSeV), Speyer, Germany."]},{"fullName":["Czajka C"],"firstName":["Christina"],"lastName":["Czajka"],"initials":["C"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany German Mosquito Control Association (KABSeV), Speyer, Germany."]},{"fullName":["Lühken R"],"firstName":["Renke"],"lastName":["Lühken"],"initials":["R"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany."]},{"fullName":["Ziegler U"],"firstName":["Ute"],"lastName":["Ziegler"],"initials":["U"],"affiliation":["Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany."]},{"fullName":["Groschup MH"],"firstName":["Martin H"],"lastName":["Groschup"],"initials":["MH"],"affiliation":["Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany."]},{"fullName":["Pfeffer M"],"firstName":["Martin"],"lastName":["Pfeffer"],"initials":["M"],"affiliation":["Faculty of Veterinary Medicine, Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany."]},{"fullName":["Becker N"],"firstName":["Norbert"],"lastName":["Becker"],"initials":["N"],"affiliation":["German Mosquito Control Association (KABSeV), Speyer, Germany."]},{"fullName":["Cadar D"],"firstName":["Daniel"],"lastName":["Cadar"],"initials":["D"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany danielcadar@gmail.com."]},{"fullName":["Schmidt-Chanasit J"],"firstName":["Jonas"],"lastName":["Schmidt-Chanasit"],"initials":["J"],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany German Centre for Infection Research (DZIF), partner site Hamburg-Luebeck-Borstel, Hamburg, Germany."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7875-4510","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["7"],"journalIssueId":["2370290"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["mBio"],"ISOAbbreviation":["MBio"],"medlineAbbreviation":["MBio"],"NLMid":["101519231"],"ESSN":["2150-7511"]}]}],"pubYear":["2016"],"pageInfo":["e01938-15"],"abstractText":["Usutu virus (USUV), one of the most neglected Old World encephalitic flaviviruses, causes epizootics among wild and captive birds and sporadic infection in humans. The dynamics of USUV spread and evolution in its natural hosts are unknown. Here, we present the phylogeny and evolutionary history of all available USUV strains, including 77 newly sequenced complete genomes from a variety of host species at a temporal and spatial scaled resolution. The results showed that USUV can be classified into six distinct lineages and that the most recent common ancestor of the recent European epizootics emerged in Africa at least 500 years ago. We demonstrated that USUV was introduced regularly from Africa into Europe in the last 50 years, and the genetic diversity of European lineages is shaped primarily by in situ evolution, while the African lineages have been driven by extensive gene flow. Most of the amino acid changes are deleterious polymorphisms removed by purifying selection, with adaptive evolution restricted to the NS5 gene and several others evolving under episodic directional selection, indicating that the ecological or immunological factors were mostly the key determinants of USUV dispersal and outbreaks. Host-specific mutations have been detected, while the host transition analysis identified mosquitoes as the most likely origin of the common ancestor and birds as the source of the recent European USUV lineages. Our results suggest that the major migratory bird flyways could predict the continental and intercontinental dispersal patterns of USUV and that migratory birds might act as potential long-distance dispersal vehicles.Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused massive bird die-offs, mostly in Europe. There is increasing evidence that USUV appears to be pathogenic for humans, becoming a potential public health problem. The emergence of USUV in Europe allows us to understand how an arbovirus spreads, adapts, and evolves in a naive environment. Thus, understanding the epidemiological and evolutionary processes that contribute to the emergence, maintenance, and further spread of viral diseases is the sine qua non to develop and implement surveillance strategies for their control. In this work, we performed an expansive phylogeographic and evolutionary analysis of USUV using all published sequences and those generated during this study. Subsequently, we described the genetic traits, reconstructed the potential pattern of geographic spread between continents/countries of the identified viral lineages and the drivers of viral migration, and traced the origin of outbreaks and transition events between different hosts."],"affiliation":["Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Research Support, Non-U.S. Gov't"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4742707?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4742707"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1128/mBio.01938-15"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"license":["cc by-nc-sa"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," pdb"," "]}],"dateOfCreation":["2016-02-03"],"dateOfRevision":["2016-02-14"],"electronicPublicationDate":["2016-02-02"],"firstPublicationDate":["2016-02-02"],"cprojectID":"PMC4742707"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSaJ9hGurOzZIXv","_score":1,"_source":{"id":["24447466"],"source":["MED"],"pmid":["24447466"],"pmcid":["PMC3901491"],"doi":["10.3201/eid2002.131620"],"title":["Novel paramyxovirus associated with severe acute febrile disease, South Sudan and Uganda, 2012."],"authorString":["Albariño CG, Foltzer M, Towner JS, Rowe LA, Campbell S, Jaramillo CM, Bird BH, Reeder DM, Vodzak ME, Rota P, Metcalfe MG, Spiropoulou CF, Knust B, Vincent JP, Frace MA, Nichol ST, Rollin PE, Ströher U."],"authorList":[{"author":[{"fullName":["Albariño CG"],"firstName":["César G"],"lastName":["Albariño"],"initials":["CG"]},{"fullName":["Foltzer M"],"firstName":["Michael"],"lastName":["Foltzer"],"initials":["M"]},{"fullName":["Towner JS"],"firstName":["Jonathan S"],"lastName":["Towner"],"initials":["JS"]},{"fullName":["Rowe LA"],"firstName":["Lory A"],"lastName":["Rowe"],"initials":["LA"]},{"fullName":["Campbell S"],"firstName":["Shelley"],"lastName":["Campbell"],"initials":["S"]},{"fullName":["Jaramillo CM"],"firstName":["Carlos M"],"lastName":["Jaramillo"],"initials":["CM"]},{"fullName":["Bird BH"],"firstName":["Brian H"],"lastName":["Bird"],"initials":["BH"]},{"fullName":["Reeder DM"],"firstName":["DeeAnn M"],"lastName":["Reeder"],"initials":["DM"]},{"fullName":["Vodzak ME"],"firstName":["Megan E"],"lastName":["Vodzak"],"initials":["ME"]},{"fullName":["Rota P"],"firstName":["Paul"],"lastName":["Rota"],"initials":["P"]},{"fullName":["Metcalfe MG"],"firstName":["Maureen G"],"lastName":["Metcalfe"],"initials":["MG"]},{"fullName":["Spiropoulou CF"],"firstName":["Christina F"],"lastName":["Spiropoulou"],"initials":["CF"]},{"fullName":["Knust B"],"firstName":["Barbara"],"lastName":["Knust"],"initials":["B"]},{"fullName":["Vincent JP"],"firstName":["Joel P"],"lastName":["Vincent"],"initials":["JP"]},{"fullName":["Frace MA"],"firstName":["Michael A"],"lastName":["Frace"],"initials":["MA"]},{"fullName":["Nichol ST"],"firstName":["Stuart T"],"lastName":["Nichol"],"initials":["ST"]},{"fullName":["Rollin PE"],"firstName":["Pierre E"],"lastName":["Rollin"],"initials":["PE"]},{"fullName":["Ströher U"],"firstName":["Ute"],"lastName":["Ströher"],"initials":["U"]}]}],"authorIdList":[{"authorId":[{"_":"0000-0001-8651-2012","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["2"],"volume":["20"],"journalIssueId":["2117745"],"dateOfPublication":["2014 Feb"],"monthOfPublication":["2"],"yearOfPublication":["2014"],"printPublicationDate":["2014-02-01"],"journal":[{"title":["Emerging infectious diseases"],"ISOAbbreviation":["Emerging Infect. Dis."],"medlineAbbreviation":["Emerg Infect Dis"],"NLMid":["9508155"],"ISSN":["1080-6040"],"ESSN":["1080-6059"]}]}],"pubYear":["2014"],"pageInfo":["211-216"],"abstractText":["In 2012, a female wildlife biologist experienced fever, malaise, headache, generalized myalgia and arthralgia, neck stiffness, and a sore throat shortly after returning to the United States from a 6-week field expedition to South Sudan and Uganda. She was hospitalized, after which a maculopapular rash developed and became confluent. When the patient was discharged from the hospital on day 14, arthralgia and myalgia had improved, oropharynx ulcerations had healed, the rash had resolved without desquamation, and blood counts and hepatic enzyme levels were returning to reference levels. After several known suspect pathogens were ruled out as the cause of her illness, deep sequencing and metagenomics analysis revealed a novel paramyxovirus related to rubula-like viruses isolated from fruit bats."],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Case Reports","Research Support, U.S. Gov't, P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Chiroptera"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Paramyxovirinae"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["IP"],"qualifierName":["isolation & purification"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Paramyxoviridae Infections"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["PA"],"qualifierName":["pathology"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["N"]},{"abbreviation":["VI"],"qualifierName":["virology"],"majorTopic_YN":["Y"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Acute Disease"]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CL"],"qualifierName":["classification"],"majorTopic_YN":["Y"]},{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Phylogeny"]},{"majorTopic_YN":["N"],"descriptorName":["Travel"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Sudan"]},{"majorTopic_YN":["N"],"descriptorName":["Uganda"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Molecular Sequence Annotation"]},{"majorTopic_YN":["N"],"descriptorName":["High-Throughput Nucleotide Sequencing"]}]}],"keywordList":[{"keyword":["Viruses","Bats","RaSH","Zoonosis","Uganda","Diagnostics","Metagenomics","South Sudan","Paramyxoviridae","Sosuga Virus","Rubula-like Virus"]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3901491?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3901491"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=24447466&action=stream&blobtype=pdf"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=24447466"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid2002.131620"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["6"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["Y"],"dbCrossReferenceList":[{"dbName":["EMBL","UNIPROT"]}],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["gen"," refseq"," "]}],"dateOfCompletion":["2014-09-02"],"dateOfCreation":["2014-01-22"],"firstPublicationDate":["2014-02-01"],"cprojectID":"PMC3901491"}}
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Direct contact is implicated as the transmission route, most likely as a sexually transmitted infection."],"affiliation":["Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1692, USA. brian.foy@colostate.edu"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Case Reports","Research Support, N.I.H., Extramural","Research Support, U.S. Gov't, P.H.S."]}],"grantsList":[{"grant":[{"grantId":["AI079528"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["R21 AI079528"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]},{"grantId":["N01-AI-25489"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Vero Cells"]},{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Cercopithecus aethiops"]},{"majorTopic_YN":["N"],"descriptorName":["Humans"]},{"majorTopic_YN":["N"],"descriptorName":["Mice"]},{"majorTopic_YN":["N"],"descriptorName":["Exanthema"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["ET"],"qualifierName":["etiology"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["RNA, Viral"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Serologic Tests"]},{"majorTopic_YN":["N"],"descriptorName":["Adult"]},{"majorTopic_YN":["N"],"descriptorName":["Colorado"]},{"majorTopic_YN":["N"],"descriptorName":["Female"]},{"majorTopic_YN":["N"],"descriptorName":["Male"]},{"majorTopic_YN":["Y"],"descriptorName":["Zika Virus"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["GE"],"qualifierName":["genetics"],"majorTopic_YN":["N"]},{"abbreviation":["PY"],"qualifierName":["pathogenicity"],"majorTopic_YN":["N"]}]}]},{"majorTopic_YN":["N"],"descriptorName":["Zika Virus Infection"],"meshQualifierList":[{"meshQualifier":[{"abbreviation":["CO"],"qualifierName":["complications"],"majorTopic_YN":["N"]},{"abbreviation":["DI"],"qualifierName":["diagnosis"],"majorTopic_YN":["N"]},{"abbreviation":["TM"],"qualifierName":["transmission"],"majorTopic_YN":["Y"]}]}]}]}],"chemicalList":[{"chemical":[{"name":["RNA, Viral"],"registryNumber":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC3321795"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["html"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=21529401"]},{"availability":["Free"],"availabilityCode":["F"],"documentStyle":["pdf"],"site":["PubMedCentral"],"url":["http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=21529401&action=stream&blobtype=pdf"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3201/eid1705.101939"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["Y"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["41"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2011-09-06"],"dateOfCreation":["2011-05-02"],"dateOfRevision":["2016-03-03"],"firstPublicationDate":["2011-05-01"],"cprojectID":"PMC3321795"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnrY-_8VqgcuJwviM","_score":1,"_source":{"id":["27154554"],"source":["MED"],"pmid":["27154554"],"pmcid":["PMC4858832"],"doi":["10.1186/s13059-016-0966-4"],"title":["Disease vectors in the era of next generation sequencing."],"authorString":["Rinker DC, Pitts RJ, Zwiebel LJ."],"authorList":[{"author":[{"fullName":["Rinker DC"],"firstName":["David C"],"lastName":["Rinker"],"initials":["DC"],"affiliation":["Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA."]},{"fullName":["Pitts RJ"],"firstName":["R Jason"],"lastName":["Pitts"],"initials":["RJ"],"affiliation":["Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA."]},{"fullName":["Zwiebel LJ"],"firstName":["Laurence J"],"lastName":["Zwiebel"],"initials":["LJ"],"affiliation":["Department of Pharmacology, Vanderbilt Brain Institute, Program in Developmental Biology, and Institutes of Chemical Biology and Global Health, Vanderbilt University Medical Center, Nashville, Tennessee, USA. l.zwiebel@vanderbilt.edu."]}]}],"journalInfo":[{"issue":["1"],"volume":["17"],"journalIssueId":["2368932"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Genome biology"],"ISOAbbreviation":["Genome Biol."],"medlineAbbreviation":["Genome Biol"],"NLMid":["100960660"],"ISSN":["1474-7596"],"ESSN":["1474-760X"]}]}],"pubYear":["2016"],"pageInfo":["95"],"abstractText":["Almost 20 % of all infectious human diseases are vector borne and, together, are responsible for over one million deaths per annum. Over the past decade, the decreasing costs of massively parallel sequencing technologies have facilitated the agnostic interrogation of insect vector genomes, giving medical entomologists access to an ever-expanding volume of high-quality genomic and transcriptomic data. In this review, we highlight how genomics resources have provided new insights into the physiology, behavior, and evolution of human disease vectors within the context of the global health landscape."],"affiliation":["Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"grantsList":[{"grant":[{"grantId":["R01 AI056402"],"agency":["NIAID NIH HHS"],"acronym":["AI"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858832?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4858832"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13059-016-0966-4"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-05-07"],"dateOfRevision":["2016-07-08"],"electronicPublicationDate":["2016-05-06"],"firstPublicationDate":["2016-05-06"],"cprojectID":"PMC4858832"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraHJ9hGurOzZIaM","_score":1,"_source":{"id":["27153063"],"source":["MED"],"pmid":["27153063"],"pmcid":["PMC4881492"],"doi":["10.3390/ijms17050666"],"title":["A Brief Review of Computer-Assisted Approaches to Rational Design of Peptide Vaccines."],"authorString":["Nandy A, Basak SC."],"authorList":[{"author":[{"fullName":["Nandy A"],"firstName":["Ashesh"],"lastName":["Nandy"],"initials":["A"],"authorId":[{"_":"0000-0002-1649-7731","$":{"type":"ORCID"}}],"affiliation":["Centre for Interdisciplinary Research and Education, Jodhpur Park, Kolkata 700068, India. anandy43@yahoo.com."]},{"fullName":["Basak SC"],"firstName":["Subhash C"],"lastName":["Basak"],"initials":["SC"],"affiliation":["Natural Resources Research Institute and Department of Chemistry & Biochemistry, University of Minnesota Duluth, Duluth, MN 55811, USA. sbasak@nrri.umn.edu."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-1649-7731","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["5"],"volume":["17"],"journalIssueId":["2401969"],"dateOfPublication":["2016"],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["International journal of molecular sciences"],"ISOAbbreviation":["Int J Mol Sci"],"medlineAbbreviation":["Int J Mol Sci"],"NLMid":["101092791"],"ESSN":["1422-0067"]}]}],"pubYear":["2016"],"abstractText":["The growing incidences of new viral diseases and increasingly frequent viral epidemics have strained therapeutic and preventive measures; the high mutability of viral genes puts additional strains on developmental efforts. Given the high cost and time requirements for new drugs development, vaccines remain as a viable alternative, but there too traditional techniques of live-attenuated or inactivated vaccines have the danger of allergenic reactions and others. Peptide vaccines have, over the last several years, begun to be looked on as more appropriate alternatives, which are economically affordable, require less time for development and hold the promise of multi-valent dosages. The developments in bioinformatics, proteomics, immunogenomics, structural biology and other sciences have spurred the growth of vaccinomics where computer assisted approaches serve to identify suitable peptide targets for eventual development of vaccines. In this mini-review we give a brief overview of some of the recent trends in computer assisted vaccine development with emphasis on the primary selection procedures of probable peptide candidates for vaccine development."],"affiliation":["Centre for Interdisciplinary Research and Education, Jodhpur Park, Kolkata 700068, India. anandy43@yahoo.com."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article","Review"]}],"keywordList":[{"keyword":["Rotavirus","Epitopes","Dengue","Chikungunya","Peptide Vaccines","Zika Virus","Alignment-free Analysis","Computer Assisted Vaccine Development","Rational Peptide Vaccine Design","Sequence Descriptors","Web Based Approaches"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4881492?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4881492"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.3390/ijms17050666"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["nct"]}],"dateOfCreation":["2016-05-07"],"dateOfRevision":["2016-05-29"],"electronicPublicationDate":["2016-05-04"],"firstPublicationDate":["2016-05-04"],"cprojectID":"PMC4881492"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnraL_8VqgcuJwviT","_score":1,"_source":{"id":["27161480"],"source":["MED"],"pmid":["27161480"],"pmcid":["PMC4862039"],"doi":["10.1186/s12864-016-2468-x"],"title":["High-throughput cis-regulatory element discovery in the vector mosquito Aedes aegypti."],"authorString":["Behura SK, Sarro J, Li P, Mysore K, Severson DW, Emrich SJ, Duman-Scheel M."],"authorList":[{"author":[{"fullName":["Behura SK"],"firstName":["Susanta K"],"lastName":["Behura"],"initials":["SK"],"affiliation":["Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA."]},{"fullName":["Sarro J"],"firstName":["Joseph"],"lastName":["Sarro"],"initials":["J"],"affiliation":["Department of Medical and Molecular Genetics, Indiana University School of Medicine, 1234 Notre Dame Ave., South Bend, IN, 46617, USA."]},{"fullName":["Li P"],"firstName":["Ping"],"lastName":["Li"],"initials":["P"],"affiliation":["Department of Medical and Molecular Genetics, Indiana University School of Medicine, 1234 Notre Dame Ave., South Bend, IN, 46617, USA."]},{"fullName":["Mysore K"],"firstName":["Keshava"],"lastName":["Mysore"],"initials":["K"],"affiliation":["Department of Medical and Molecular Genetics, Indiana University School of Medicine, 1234 Notre Dame Ave., South Bend, IN, 46617, USA."]},{"fullName":["Severson DW"],"firstName":["David W"],"lastName":["Severson"],"initials":["DW"],"affiliation":["Department of Medical and Molecular Genetics, Indiana University School of Medicine, 1234 Notre Dame Ave., South Bend, IN, 46617, USA."]},{"fullName":["Emrich SJ"],"firstName":["Scott J"],"lastName":["Emrich"],"initials":["SJ"],"affiliation":["Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA. semrich@nd.edu."]},{"fullName":["Duman-Scheel M"],"firstName":["Molly"],"lastName":["Duman-Scheel"],"initials":["M"],"affiliation":["Department of Medical and Molecular Genetics, Indiana University School of Medicine, 1234 Notre Dame Ave., South Bend, IN, 46617, USA. mscheel@nd.edu."]}]}],"journalInfo":[{"volume":["17"],"journalIssueId":["2401365"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["BMC genomics"],"ISOAbbreviation":["BMC Genomics"],"medlineAbbreviation":["BMC Genomics"],"NLMid":["100965258"],"ESSN":["1471-2164"]}]}],"pubYear":["2016"],"pageInfo":["341"],"abstractText":["Despite substantial progress in mosquito genomic and genetic research, few cis-regulatory elements (CREs), DNA sequences that control gene expression, have been identified in mosquitoes or other non-model insects. Formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, FAIRE-seq, is emerging as a powerful new high-throughput tool for global CRE discovery. FAIRE results in the preferential recovery of open chromatin DNA fragments that are not bound by nucleosomes, an evolutionarily conserved indicator of regulatory activity, which are then sequenced. Despite the power of the approach, FAIRE-seq has not yet been applied to the study of non-model insects. In this investigation, we utilized FAIRE-seq to profile open chromatin and identify likely regulatory elements throughout the genome of the human disease vector mosquito Aedes aegypti. We then assessed genetic variation in the regulatory elements of dengue virus susceptible (Moyo-S) and refractory (Moyo-R) mosquito strains.Analysis of sequence data obtained through next generation sequencing of FAIRE DNA isolated from A. aegypti embryos revealed >121,000 FAIRE peaks (FPs), many of which clustered in the 1 kb 5' upstream flanking regions of genes known to be expressed at this stage. As expected, known transcription factor consensus binding sites were enriched in the FPs, and of these FoxA1, Hunchback, Gfi, Klf4, MYB/ph3 and Sox9 are most predominant. All of the elements tested in vivo were confirmed to drive gene expression in transgenic Drosophila reporter assays. Of the >13,000 single nucleotide polymorphisms (SNPs) recently identified in dengue virus-susceptible and refractory mosquito strains, 3365 were found to map to FPs.FAIRE-seq analysis of open chromatin in A. aegypti permitted genome-wide discovery of CREs. The results of this investigation indicate that FAIRE-seq is a powerful tool for identification of regulatory DNA in the genomes of non-model organisms, including human disease vector mosquitoes."],"affiliation":["Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Genome","Drosophila","Dengue virus","Next Generation Sequencing","Faire-seq","Zika"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4862039?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC4862039"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s12864-016-2468-x"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["Y"],"tmAccessionTypeList":[{"accessionType":["bioproject"]}],"dateOfCreation":["2016-05-10"],"dateOfRevision":["2016-05-12"],"electronicPublicationDate":["2016-05-10"],"firstPublicationDate":["2016-05-10"],"cprojectID":"PMC4862039"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsMo_8VqgcuJwvk4","_score":1,"_source":{"id":["27436286"],"source":["MED"],"pmid":["27436286"],"pmcid":["PMC5009743"],"doi":["10.1093/nar/gkw530"],"title":["Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use."],"authorString":["Atkins JF, Loughran G, Bhatt PR, Firth AE, Baranov PV."],"authorList":[{"author":[{"fullName":["Atkins JF"],"firstName":["John F"],"lastName":["Atkins"],"initials":["JF"],"affiliation":["School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland School of Microbiology, University College Cork, Cork, Ireland Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA j.atkins@ucc.ie."]},{"fullName":["Loughran G"],"firstName":["Gary"],"lastName":["Loughran"],"initials":["G"],"authorId":[{"_":"0000-0002-2683-5597","$":{"type":"ORCID"}}],"affiliation":["School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland."]},{"fullName":["Bhatt PR"],"firstName":["Pramod R"],"lastName":["Bhatt"],"initials":["PR"],"affiliation":["School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland."]},{"fullName":["Firth AE"],"firstName":["Andrew E"],"lastName":["Firth"],"initials":["AE"],"affiliation":["Division of Virology, Department of Pathology, University of Cambridge, Hills Road, Cambridge CB2 0QQ, UK."]},{"fullName":["Baranov PV"],"firstName":["Pavel V"],"lastName":["Baranov"],"initials":["PV"],"affiliation":["School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-2683-5597","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["15"],"volume":["44"],"journalIssueId":["2446222"],"dateOfPublication":["2016 Sep"],"monthOfPublication":["9"],"yearOfPublication":["2016"],"printPublicationDate":["2016-09-01"],"journal":[{"title":["Nucleic acids research"],"ISOAbbreviation":["Nucleic Acids Res."],"medlineAbbreviation":["Nucleic Acids Res"],"NLMid":["0411011"],"ISSN":["0305-1048"],"ESSN":["1362-4962"]}]}],"pubYear":["2016"],"pageInfo":["7007-7078"],"abstractText":["Genetic decoding is not 'frozen' as was earlier thought, but dynamic. One facet of this is frameshifting that often results in synthesis of a C-terminal region encoded by a new frame. Ribosomal frameshifting is utilized for the synthesis of additional products, for regulatory purposes and for translational 'correction' of problem or 'savior' indels. Utilization for synthesis of additional products occurs prominently in the decoding of mobile chromosomal element and viral genomes. One class of regulatory frameshifting of stable chromosomal genes governs cellular polyamine levels from yeasts to humans. In many cases of productively utilized frameshifting, the proportion of ribosomes that frameshift at a shift-prone site is enhanced by specific nascent peptide or mRNA context features. Such mRNA signals, which can be 5' or 3' of the shift site or both, can act by pairing with ribosomal RNA or as stem loops or pseudoknots even with one component being 4 kb 3' from the shift site. Transcriptional realignment at slippage-prone sequences also generates productively utilized products encoded trans-frame with respect to the genomic sequence. This too can be enhanced by nucleic acid structure. Together with dynamic codon redefinition, frameshifting is one of the forms of recoding that enriches gene expression."],"affiliation":["School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland School of Microbiology, University College Cork, Cork, Ireland Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA j.atkins@ucc.ie."],"language":["eng"],"pubModel":["Print-Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"grantsList":[{"grant":[{"grantId":["646891"],"agency":["European Research Council"],"orderIn":["0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009743?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5009743"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1093/nar/gkw530"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-03"],"dateOfRevision":["2016-09-07"],"electronicPublicationDate":["2016-07-19"],"firstPublicationDate":["2016-07-19"],"cprojectID":"PMC5009743"}}
{"_index":"metadata","_type":"eupmc","_id":"AVdDnsMt_8VqgcuJwvk5","_score":1,"_source":{"id":["27595597"],"source":["MED"],"pmid":["27595597"],"pmcid":["PMC5012000"],"doi":["10.1186/s13071-016-1763-3"],"title":["Isolation of fungi from dead arthropods and identification of a new mosquito natural pathogen."],"authorString":["Jaber S, Mercier A, Knio K, Brun S, Kambris Z."],"authorList":[{"author":[{"fullName":["Jaber S"],"firstName":["Sana"],"lastName":["Jaber"],"initials":["S"],"affiliation":["Biology Department, American University of Beirut, Beirut, Lebanon."]},{"fullName":["Mercier A"],"firstName":["Alex"],"lastName":["Mercier"],"initials":["A"],"affiliation":["Equipe Genetique et Epigenetique des Champignons, LIED, UMR8236, Universite Paris-Diderot, Paris, France."]},{"fullName":["Knio K"],"firstName":["Khouzama"],"lastName":["Knio"],"initials":["K"],"affiliation":["Biology Department, American University of Beirut, Beirut, Lebanon."]},{"fullName":["Brun S"],"firstName":["Sylvain"],"lastName":["Brun"],"initials":["S"],"affiliation":["Equipe Genetique et Epigenetique des Champignons, LIED, UMR8236, Universite Paris-Diderot, Paris, France. sylvain.brun@univ-paris-diderot.fr."]},{"fullName":["Kambris Z"],"firstName":["Zakaria"],"lastName":["Kambris"],"initials":["Z"],"authorId":[{"_":"0000-0002-7377-1899","$":{"type":"ORCID"}}],"affiliation":["Biology Department, American University of Beirut, Beirut, Lebanon. zk28@aub.edu.lb."]}]}],"authorIdList":[{"authorId":[{"_":"0000-0002-7377-1899","$":{"type":"ORCID"}}]}],"journalInfo":[{"issue":["1"],"volume":["9"],"journalIssueId":["2358895"],"dateOfPublication":["2016 "],"monthOfPublication":["0"],"yearOfPublication":["2016"],"printPublicationDate":["2016-01-01"],"journal":[{"title":["Parasites & vectors"],"ISOAbbreviation":["Parasit Vectors"],"medlineAbbreviation":["Parasit Vectors"],"NLMid":["101462774"],"ESSN":["1756-3305"]}]}],"pubYear":["2016"],"pageInfo":["491"],"abstractText":["Insects are well known vectors of human and animal pathogens and millions of people are killed by mosquito-borne diseases every year. The use of insecticides to target insect vectors has been hampered by the issues of toxicity to the environment and by the selection of resistant insects. Therefore, biocontrol strategies based on naturally occurring microbial pathogens emerged as a promising control alternative. The entomopathogenic fungus Beauveria bassiana is well characterized and have been approved by the United States Environmental Protection Agency as a pest biological control method. However, thousands of other fungi are unexploited and it is important to identify and use different fungi for biocontrol with possibly some vector specific strains. The aim of this study was to identify new fungal entomopathogens that may be used as potential mosquito biocontrol agents.Cadavers of arthropods were collected from pesticide free areas and the fungi associated isolated, cultured and identified. Then the ability of each isolate to kill laboratory insects was assayed and compared to that of B. bassiana.In total we have isolated and identified 42 fungal strains from 17 different arthropod cadavers. Twenty four fungal isolates were cultivated in the laboratory and were able to induce sporulation. When fungal spores were microinjected into Drosophila melanogaster, eight isolates proved to be highly pathogenic while the remaining strains showed moderate or no pathogenicity. Then a selection of isolates was tested against Aedes mosquitoes in a model mimicking natural infections. Only one fungus (Aspergillus nomius) was as pathogenic as B. bassiana and able to kill 100 % of the mosquitoes.The obtained results are encouraging and demonstrate the feasibility of this simple approach for the identification of new potential mosquito killers. Indeed, it is essential to anticipate and prepare biocontrol methods to fight the expansion of mosquitoes' habitat predicted in certain geographical areas in association with the occurring climatic changes."],"affiliation":["Biology Department, American University of Beirut, Beirut, Lebanon."],"language":["eng"],"pubModel":["Electronic"],"pubTypeList":[{"pubType":["Journal Article"]}],"keywordList":[{"keyword":["Fungi","Infection","Beauveria bassiana","Drosophila","Aedes","Vector Control","Entomopathogen"]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5012000?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC5012000"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1186/s13071-016-1763-3"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["0"],"hasReferences":["N"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["Y"],"license":["cc by"],"hasTMAccessionNumbers":["N"],"dateOfCreation":["2016-09-06"],"dateOfRevision":["2016-09-08"],"electronicPublicationDate":["2016-09-05"],"firstPublicationDate":["2016-09-05"],"cprojectID":"PMC5012000"}}
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{"_index":"metadata","_type":"eupmc","_id":"AVdDnqSfhOUS2orL1IN_","_score":1,"_source":{"id":["11677178"],"source":["MED"],"pmid":["11677178"],"pmcid":["PMC1240600"],"doi":["10.1289/ehp.01109s5699"],"title":["The role of nutrient loading and eutrophication in estuarine ecology."],"authorString":["Pinckney JL, Paerl HW, Tester P, Richardson TL."],"authorList":[{"author":[{"fullName":["Pinckney JL"],"lastName":["Pinckney"],"initials":["JL"]},{"fullName":["Paerl HW"],"lastName":["Paerl"],"initials":["HW"]},{"fullName":["Tester P"],"lastName":["Tester"],"initials":["P"]},{"fullName":["Richardson TL"],"lastName":["Richardson"],"initials":["TL"]}]}],"journalInfo":[{"volume":["109 Suppl 5"],"journalIssueId":["1023570"],"dateOfPublication":["2001 Oct"],"monthOfPublication":["10"],"yearOfPublication":["2001"],"printPublicationDate":["2001-10-01"],"journal":[{"title":["Environmental health perspectives"],"ISOAbbreviation":["Environ. Health Perspect."],"medlineAbbreviation":["Environ Health Perspect"],"NLMid":["0330411"],"ISSN":["0091-6765"],"ESSN":["1552-9924"]}]}],"pubYear":["2001"],"pageInfo":["699-706"],"abstractText":["Eutrophication is a process that can be defined as an increase in the rate of supply of organic matter (OM) to an ecosystem. We provide a general overview of the major features driving estuarine eutrophication and outline some of the consequences of that process. The main chemical constituent of OM is carbon (C), and therefore rates of eutrophication are expressed in units of C per area per unit time. OM occurs in both particulate and dissolved forms. Allochthonous OM originates outside the estuary, whereas autochthonous OM is generated within the system, mostly by primary producers or by benthic regeneration of OM. The supply rates of limiting nutrients regulate phytoplankton productivity that contributes to inputs of autochthonous OM. The trophic status of an estuary is often based on eutrophication rates and can be categorized as oligotrophic (<100 g C m(-2) y(-1), mesotrophic (100-300 g C m(-2) y(-1), eutrophic (300-500 g C m(-2) y(-1), or hypertrophic (>500 g C m(-2) y(-1). Ecosystem responses to eutrophication depend on both export rates (flushing, microbially mediated losses through respiration, and denitrification) and recycling/regeneration rates within the estuary. The mitigation of the effects of eutrophication involves the regulation of inorganic nutrient (primarily N and P) inputs into receiving waters. Appropriately scaled and parameterized nutrient and hydrologic controls are the only realistic options for controlling phytoplankton blooms, algal toxicity, and other symptoms of eutrophication in estuarine ecosystems."],"affiliation":["Department of Oceanography, Texas A&M University, College Station 77843, USA. pinckney@ocean.tamu.edu"],"language":["eng"],"pubModel":["Print"],"pubTypeList":[{"pubType":["Journal Article","Review","Research Support, Non-U.S. Gov't","Research Support, U.S. Gov't, Non-P.H.S."]}],"meshHeadingList":[{"meshHeading":[{"majorTopic_YN":["N"],"descriptorName":["Animals"]},{"majorTopic_YN":["N"],"descriptorName":["Zooplankton"]},{"majorTopic_YN":["N"],"descriptorName":["Phytoplankton"]},{"majorTopic_YN":["Y"],"descriptorName":["Nitrogen"]},{"majorTopic_YN":["Y"],"descriptorName":["Phosphorus"]},{"majorTopic_YN":["N"],"descriptorName":["Water Microbiology"]},{"majorTopic_YN":["Y"],"descriptorName":["Ecosystem"]},{"majorTopic_YN":["N"],"descriptorName":["Population Dynamics"]},{"majorTopic_YN":["N"],"descriptorName":["Water Supply"]},{"majorTopic_YN":["Y"],"descriptorName":["Eutrophication"]}]}],"chemicalList":[{"chemical":[{"name":["Nitrogen"],"registryNumber":["7727-37-9"]},{"name":["Phosphorus"],"registryNumber":["7723-14-0"]}]}],"subsetList":[{"subset":[{"code":["IM"],"name":["Index Medicus"]}]}],"fullTextUrlList":[{"fullTextUrl":[{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["pdf"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1240600?pdf=render"]},{"availability":["Open access"],"availabilityCode":["OA"],"documentStyle":["html"],"site":["Europe_PMC"],"url":["http://europepmc.org/articles/PMC1240600"]},{"availability":["Subscription required"],"availabilityCode":["S"],"documentStyle":["doi"],"site":["DOI"],"url":["http://dx.doi.org/10.1289/ehp.01109s5699"]}]}],"isOpenAccess":["Y"],"inEPMC":["Y"],"inPMC":["N"],"hasPDF":["Y"],"hasBook":["N"],"hasSuppl":["N"],"citedByCount":["5"],"hasReferences":["Y"],"hasTextMinedTerms":["Y"],"hasDbCrossReferences":["N"],"hasLabsLinks":["N"],"hasTMAccessionNumbers":["N"],"dateOfCompletion":["2003-07-08"],"dateOfCreation":["2001-10-25"],"dateOfRevision":["2010-09-14"],"firstPublicationDate":["2001-10-01"],"cprojectID":"PMC1240600"}}