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LIN28 selectively modulates a subclass of let-7 microRNAs

Ustinanenko, Dmytro; Chiu, Hua-Sheng; Treiber, Thomas; Weyn-Vanhentenryck, Sebastien M; Treiber, Nora; Meister, Gunter; Sumazin, Pavel; Zhang, Chaolin


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{
  "inLanguage": {
    "alternateName": "eng", 
    "@type": "Language", 
    "name": "English"
  }, 
  "description": "<p>LIN28 is a bipartite RNA-binding protein that post-transcriptionally inhibits the biogenesis of let-7 microRNAs to regulate development and influence disease states. However, the mechanisms of let-7 suppression remains poorly understood, because LIN28 recognition depends on coordinated targeting by both the zinc knuckle domain (ZKD) &mdash;which binds a GGAG-like element in the precursor&mdash;and the cold shock domain (CSD), whose binding sites have not been systematically characterized. By leveraging single-nucleotide-resolution mapping of LIN28 binding sites in vivo, we determined that the CSD recognizes a (U)GAU motif. This motif partitions the let-7 microRNAs into two subclasses, precursors with both CSD and ZKD binding sites (CSD+) and precursors with ZKD but no CSD binding sites (CSD-). LIN28 in vivo recognition&mdash;and subsequent 3\u02b9 uridylation and degradation&mdash;of CSD+ precursors is more efficient, leading to their stronger suppression in LIN28-activated cells and cancers. Thus, CSD binding sites amplify the effects of the LIN28 activation.</p>", 
  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA", 
      "@type": "Person", 
      "name": "Ustinanenko, Dmytro"
    }, 
    {
      "affiliation": "Texas Children's Cancer Center, Department of Pediatrics, and Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA", 
      "@type": "Person", 
      "name": "Chiu, Hua-Sheng"
    }, 
    {
      "affiliation": "Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany", 
      "@type": "Person", 
      "name": "Treiber, Thomas"
    }, 
    {
      "affiliation": "Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA", 
      "@type": "Person", 
      "name": "Weyn-Vanhentenryck, Sebastien M"
    }, 
    {
      "affiliation": "Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany", 
      "@type": "Person", 
      "name": "Treiber, Nora"
    }, 
    {
      "affiliation": "Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany", 
      "@type": "Person", 
      "name": "Meister, Gunter"
    }, 
    {
      "affiliation": "Texas Children's Cancer Center, Department of Pediatrics, and Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA", 
      "@type": "Person", 
      "name": "Sumazin, Pavel"
    }, 
    {
      "affiliation": "Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA", 
      "@type": "Person", 
      "name": "Zhang, Chaolin"
    }
  ], 
  "url": "https://zenodo.org/record/1445985", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "2018-07-18", 
  "headline": "LIN28 selectively modulates a subclass of let-7 microRNAs", 
  "keywords": [
    "LIN28", 
    "let-7 microRNA biogenesis", 
    "cold shock domani", 
    "bipartite binding", 
    "selective suppression"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1016/j.molcel.2018.06.029", 
  "@id": "https://doi.org/10.1016/j.molcel.2018.06.029", 
  "@type": "ScholarlyArticle", 
  "name": "LIN28 selectively modulates a subclass of let-7 microRNAs"
}
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