Journal article Open Access

LIN28 selectively modulates a subclass of let-7 microRNAs

Ustinanenko, Dmytro; Chiu, Hua-Sheng; Treiber, Thomas; Weyn-Vanhentenryck, Sebastien M; Treiber, Nora; Meister, Gunter; Sumazin, Pavel; Zhang, Chaolin


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://zenodo.org/record/1445985</identifier>
  <creators>
    <creator>
      <creatorName>Ustinanenko, Dmytro</creatorName>
      <givenName>Dmytro</givenName>
      <familyName>Ustinanenko</familyName>
      <affiliation>Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA</affiliation>
    </creator>
    <creator>
      <creatorName>Chiu, Hua-Sheng</creatorName>
      <givenName>Hua-Sheng</givenName>
      <familyName>Chiu</familyName>
      <affiliation>Texas Children's Cancer Center, Department of Pediatrics, and Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA</affiliation>
    </creator>
    <creator>
      <creatorName>Treiber, Thomas</creatorName>
      <givenName>Thomas</givenName>
      <familyName>Treiber</familyName>
      <affiliation>Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Weyn-Vanhentenryck, Sebastien M</creatorName>
      <givenName>Sebastien M</givenName>
      <familyName>Weyn-Vanhentenryck</familyName>
      <affiliation>Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA</affiliation>
    </creator>
    <creator>
      <creatorName>Treiber, Nora</creatorName>
      <givenName>Nora</givenName>
      <familyName>Treiber</familyName>
      <affiliation>Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Meister, Gunter</creatorName>
      <givenName>Gunter</givenName>
      <familyName>Meister</familyName>
      <affiliation>Biochemistry Center Regensburg (BZR), Laboratory for RNA Biology, University of Regensburg, 93053 Regensburg, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Sumazin, Pavel</creatorName>
      <givenName>Pavel</givenName>
      <familyName>Sumazin</familyName>
      <affiliation>Texas Children's Cancer Center, Department of Pediatrics, and Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA</affiliation>
    </creator>
    <creator>
      <creatorName>Zhang, Chaolin</creatorName>
      <givenName>Chaolin</givenName>
      <familyName>Zhang</familyName>
      <affiliation>Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA</affiliation>
    </creator>
  </creators>
  <titles>
    <title>LIN28 selectively modulates a subclass of let-7 microRNAs</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <subjects>
    <subject>LIN28</subject>
    <subject>let-7 microRNA biogenesis</subject>
    <subject>cold shock domani</subject>
    <subject>bipartite binding</subject>
    <subject>selective suppression</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2018-07-18</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1445985</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.molcel.2018.06.029</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/precise</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;LIN28 is a bipartite RNA-binding protein that post-transcriptionally inhibits the biogenesis of let-7 microRNAs to regulate development and influence disease states. However, the mechanisms of let-7 suppression remains poorly understood, because LIN28 recognition depends on coordinated targeting by both the zinc knuckle domain (ZKD) &amp;mdash;which binds a GGAG-like element in the precursor&amp;mdash;and the cold shock domain (CSD), whose binding sites have not been systematically characterized. By leveraging single-nucleotide-resolution mapping of LIN28 binding sites in vivo, we determined that the CSD recognizes a (U)GAU motif. This motif partitions the let-7 microRNAs into two subclasses, precursors with both CSD and ZKD binding sites (CSD+) and precursors with ZKD but no CSD binding sites (CSD-). LIN28 in vivo recognition&amp;mdash;and subsequent 3ʹ uridylation and degradation&amp;mdash;of CSD+ precursors is more efficient, leading to their stronger suppression in LIN28-activated cells and cancers. Thus, CSD binding sites amplify the effects of the LIN28 activation.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/501100000780</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/668858/">668858</awardNumber>
      <awardTitle>PERSONALIZED ENGINE FOR CANCER INTEGRATIVE STUDY AND EVALUATION</awardTitle>
    </fundingReference>
  </fundingReferences>
</resource>
20
12
views
downloads
Views 20
Downloads 12
Data volume 9.0 MB
Unique views 19
Unique downloads 11

Share

Cite as