[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.
[Single Cell] - After imaging with a microscope to identify which sites have captured a single cell, processing of the cells occurs within the C1 instrument to perform the steps of cell lysis, cDNA synthesis with reverse transcriptase, and PCR amplification of each cDNA library.  The cDNA synthesis and PCR use reagents from the SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing (Clontech 634936).  The SMARTer chemistry utilizes a strand-switching mechanism so that both the 1st and 2nd strands of cDNA are synthesized in a single reaction.  Following harvest from the C1 microfluidic device, each cDNA library is subjected to tagmentation (simultaneous fragmentation and tagging with sequencing adapters) using the Nextera XT DNA Sample Preparation Kit (Illumina FC-131-1096) as described in Fluidigm protocol 100-5950.  PCR amplification of the tagmented cDNA uses Index Primers from the Nextera XT DNA Sample Preparation Index Kit (Illumina FC-131-1002).  Following PCR, the cDNA libraries from individual cells are pooled and purified using AMPure® XP beads (Agencourt Bioscience Corp A63880) as described in Fluidigm protocol 100-5950.