Journal article Open Access
Paudel, Niroj; Aryal, Mukti Ram; Puri, Rudra Hari
Asparagus racemosus Wild. is an undershrub, climber which is extremely branched with woody stem and spines like reduced leaves. It founds in tropical and sub-tropical region of Nepal up to 1500 meter above sea level. A. racemosus Wild. is an endangered species widely used in modern drug development and Ayurveda as well. The subculture explant shows responses to the different concentration of hormone. The composition of MS + 0.5 mg/l BAP and MS + NAA 0.5 mg/l + kinetin 1 mg/l most significant for shoot multiplication. Over them, the composition MS = 0.01 mg/l NAA + 1.0 mg/l BAP along with MS + 0.5 mg/l NAA = 1.5 mg/l kinetin is effective for the development of shoot in Asparagus racemosus. The hormone play important role on the regeneration of shoot in vitro condition. The knowledge of hormonal requirement help to promote the development and growth of endangered plants for their rapid propagation.