Author: University of Nottingham Medical School Division of Histopathy    

METHOD:

  1. Wash glass slides in detergent for 30 minutes.
  • Wash glass slides in running tap water for 30 minutes.
  • Wash glass slides in distilled water 2x5 minutes.
  • Wash glass slides in 95% alcohol 2x5 minutes.
  • Air dry for 10 minutes.
  • Immerse slides in a freshly prepared 2% solution for 3-aminopropyltriethoxysilane in dry acetone for 5 seconds.
  • Wash briefly in distilled water twice.
  • Dry overnight at 42C and store at room temperature.

    • Note: 300mls of silane solution is sufficient to do 200 slides. Treated slides can be kept indefinitely.