Presentation Open Access

An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila - sequence files

Li-Kroeger, David; Kanca, Oguz; Lee, Pei-Tseng; Cowan, Sierra; Lee, Michael T; Jaiswal, Manish; Salazar, Jose Luis; He, Yuchun; Zuo, Zhongyuan; Bellen, Hugo J.


MARC21 XML Export

<?xml version='1.0' encoding='UTF-8'?>
<record xmlns="http://www.loc.gov/MARC21/slim">
  <leader>00000nam##2200000uu#4500</leader>
  <datafield tag="653" ind1=" " ind2=" ">
    <subfield code="a">Double Header, gene editing, T2A-GAL4, RMCE, protein tagging, Trojan exons, swappable insertion cassette (SIC), Nmnat</subfield>
  </datafield>
  <controlfield tag="005">20200120173228.0</controlfield>
  <datafield tag="500" ind1=" " ind2=" ">
    <subfield code="a">Funding:  Howard Hughes Medical Institute; National Institutes of Health U54HD083092, R01GM067858, T32NS043124; Robert A and Renee E Belfer Family Foundation</subfield>
  </datafield>
  <controlfield tag="001">1341241</controlfield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Baylor College of Medicine</subfield>
    <subfield code="0">(orcid)0000-0001-5438-0879</subfield>
    <subfield code="a">Kanca, Oguz</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Baylor College of Medicine</subfield>
    <subfield code="0">(orcid)0000-0002-7501-7881</subfield>
    <subfield code="a">Lee, Pei-Tseng</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Rice University</subfield>
    <subfield code="0">(orcid)0000-0003-3530-9326</subfield>
    <subfield code="a">Cowan, Sierra</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Rice University</subfield>
    <subfield code="a">Lee, Michael T</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">HHMI, Baylor College of Medicine</subfield>
    <subfield code="a">Jaiswal, Manish</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Baylor College of Medicine</subfield>
    <subfield code="a">Salazar, Jose Luis</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">HHMI, Baylor College of Medicine</subfield>
    <subfield code="a">He, Yuchun</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">Baylor College of Medicine</subfield>
    <subfield code="0">(orcid)0000-0003-4563-7921</subfield>
    <subfield code="a">Zuo, Zhongyuan</subfield>
  </datafield>
  <datafield tag="700" ind1=" " ind2=" ">
    <subfield code="u">HHMI, Baylor College of Medicine</subfield>
    <subfield code="0">(orcid)0000-0001-5992-5989</subfield>
    <subfield code="a">Bellen, Hugo J.</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">8252476637</subfield>
    <subfield code="z">md5:969a9d1528cf4206c2877ba1393c9e3e</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/iso2_18.local_realigned.sorted.bam</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">458144</subfield>
    <subfield code="z">md5:67a93d7549c153d208a2f509db8722c3</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/iso2_18.local_realigned.sorted.bam.bai</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">6239126860</subfield>
    <subfield code="z">md5:96e7cb14988acac55b9cf965f8ed16c9</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/iso3_5.local_realigned.sorted.bam</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">454168</subfield>
    <subfield code="z">md5:37949f66f1c9ff37982acdc7d12d6794</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/iso3_5.local_realigned.sorted.bam.bai</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">8225032905</subfield>
    <subfield code="z">md5:c28af7cb87700a09adc9e1ba3883d022</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/isoX_6.local_realigned.sorted.bam</subfield>
  </datafield>
  <datafield tag="856" ind1="4" ind2=" ">
    <subfield code="s">457328</subfield>
    <subfield code="z">md5:0933af13ace8a35ad701ba5e5e8901c9</subfield>
    <subfield code="u">https://zenodo.org/record/1341241/files/isoX_6.local_realigned.sorted.bam.bai</subfield>
  </datafield>
  <datafield tag="542" ind1=" " ind2=" ">
    <subfield code="l">open</subfield>
  </datafield>
  <datafield tag="260" ind1=" " ind2=" ">
    <subfield code="c">2018-08-09</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="O">
    <subfield code="p">openaire</subfield>
    <subfield code="o">oai:zenodo.org:1341241</subfield>
  </datafield>
  <datafield tag="909" ind1="C" ind2="4">
    <subfield code="p">eLife</subfield>
  </datafield>
  <datafield tag="100" ind1=" " ind2=" ">
    <subfield code="u">Baylor College of Medicine</subfield>
    <subfield code="a">Li-Kroeger, David</subfield>
  </datafield>
  <datafield tag="245" ind1=" " ind2=" ">
    <subfield code="a">An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila - sequence files</subfield>
  </datafield>
  <datafield tag="540" ind1=" " ind2=" ">
    <subfield code="u">https://creativecommons.org/licenses/by/4.0/legalcode</subfield>
    <subfield code="a">Creative Commons Attribution 4.0 International</subfield>
  </datafield>
  <datafield tag="650" ind1="1" ind2="7">
    <subfield code="a">cc-by</subfield>
    <subfield code="2">opendefinition.org</subfield>
  </datafield>
  <datafield tag="520" ind1=" " ind2=" ">
    <subfield code="a">&lt;p&gt;In this work, Cas9 stocks for CRISPR experiments that carried an isogenic chromosome on either X, 2 or 3 derived from &lt;em&gt;y w&lt;/em&gt; and were either &lt;em&gt;y&lt;sup&gt;1&lt;/sup&gt; M{nos-Cas9.P}ZH-2A w* &lt;/em&gt;(from Bloomington Drosophila Stock Center) or &lt;em&gt;y&lt;sup&gt;1&lt;/sup&gt; (iso X) w&lt;sup&gt;*&lt;/sup&gt;; +/+; &lt;/em&gt;&lt;em&gt;attP2(y+){nos-Cas9(y+)}&lt;/em&gt; (Ren et al., 2013) in which the &lt;em&gt;y&lt;/em&gt;+ marker was mutagenized by injecting sgRNA expression plasmids (pCFD3-y1 and pCFD3-y2) against the &lt;em&gt;yellow&lt;/em&gt; coding sequence.&amp;nbsp; These are the sequence files (.bam) and indexes (.bai) for the isogenic chromosomes (X, 2, 3) which were sequenced using whole genome sequencing (Human Genome Sequencing Center at Baylor College of Medicine).&lt;/p&gt;

&lt;p&gt;Information on BAM files:&lt;/p&gt;

&lt;p&gt;&lt;a href="http://software.broadinstitute.org/software/igv/BAM"&gt;http://software.broadinstitute.org/software/igv/BAM&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;&lt;a href="http://genome.ucsc.edu/goldenPath/help/bam.html"&gt;http://genome.ucsc.edu/goldenPath/help/bam.html&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;SAMtools download:&lt;/p&gt;

&lt;p&gt;&lt;a href="http://samtools.sourceforge.net/"&gt;http://samtools.sourceforge.net/&lt;/a&gt;&lt;/p&gt;</subfield>
  </datafield>
  <datafield tag="024" ind1=" " ind2=" ">
    <subfield code="a">10.7554/eLife.38709</subfield>
    <subfield code="2">doi</subfield>
  </datafield>
  <datafield tag="980" ind1=" " ind2=" ">
    <subfield code="a">presentation</subfield>
  </datafield>
</record>
193
49
views
downloads
Views 193
Downloads 49
Data volume 233.4 GB
Unique views 169
Unique downloads 28

Share

Cite as