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An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila - sequence files

Li-Kroeger, David; Kanca, Oguz; Lee, Pei-Tseng; Cowan, Sierra; Lee, Michael T; Jaiswal, Manish; Salazar, Jose Luis; He, Yuchun; Zuo, Zhongyuan; Bellen, Hugo J.


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{
  "description": "<p>In this work, Cas9 stocks for CRISPR experiments that carried an isogenic chromosome on either X, 2 or 3 derived from <em>y w</em> and were either <em>y<sup>1</sup> M{nos-Cas9.P}ZH-2A w* </em>(from Bloomington Drosophila Stock Center) or <em>y<sup>1</sup> (iso X) w<sup>*</sup>; +/+; </em><em>attP2(y+){nos-Cas9(y+)}</em> (Ren et al., 2013) in which the <em>y</em>+ marker was mutagenized by injecting sgRNA expression plasmids (pCFD3-y1 and pCFD3-y2) against the <em>yellow</em> coding sequence.&nbsp; These are the sequence files (.bam) and indexes (.bai) for the isogenic chromosomes (X, 2, 3) which were sequenced using whole genome sequencing (Human Genome Sequencing Center at Baylor College of Medicine).</p>\n\n<p>Information on BAM files:</p>\n\n<p><a href=\"http://software.broadinstitute.org/software/igv/BAM\">http://software.broadinstitute.org/software/igv/BAM</a></p>\n\n<p><a href=\"http://genome.ucsc.edu/goldenPath/help/bam.html\">http://genome.ucsc.edu/goldenPath/help/bam.html</a></p>\n\n<p>SAMtools download:</p>\n\n<p><a href=\"http://samtools.sourceforge.net/\">http://samtools.sourceforge.net/</a></p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Baylor College of Medicine", 
      "@type": "Person", 
      "name": "Li-Kroeger, David"
    }, 
    {
      "affiliation": "Baylor College of Medicine", 
      "@id": "https://orcid.org/0000-0001-5438-0879", 
      "@type": "Person", 
      "name": "Kanca, Oguz"
    }, 
    {
      "affiliation": "Baylor College of Medicine", 
      "@id": "https://orcid.org/0000-0002-7501-7881", 
      "@type": "Person", 
      "name": "Lee, Pei-Tseng"
    }, 
    {
      "affiliation": "Rice University", 
      "@id": "https://orcid.org/0000-0003-3530-9326", 
      "@type": "Person", 
      "name": "Cowan, Sierra"
    }, 
    {
      "affiliation": "Rice University", 
      "@type": "Person", 
      "name": "Lee, Michael T"
    }, 
    {
      "affiliation": "HHMI, Baylor College of Medicine", 
      "@type": "Person", 
      "name": "Jaiswal, Manish"
    }, 
    {
      "affiliation": "Baylor College of Medicine", 
      "@type": "Person", 
      "name": "Salazar, Jose Luis"
    }, 
    {
      "affiliation": "HHMI, Baylor College of Medicine", 
      "@type": "Person", 
      "name": "He, Yuchun"
    }, 
    {
      "affiliation": "Baylor College of Medicine", 
      "@id": "https://orcid.org/0000-0003-4563-7921", 
      "@type": "Person", 
      "name": "Zuo, Zhongyuan"
    }, 
    {
      "affiliation": "HHMI, Baylor College of Medicine", 
      "@id": "https://orcid.org/0000-0001-5992-5989", 
      "@type": "Person", 
      "name": "Bellen, Hugo J."
    }
  ], 
  "url": "https://zenodo.org/record/1341241", 
  "datePublished": "2018-08-09", 
  "keywords": [
    "Double Header, gene editing, T2A-GAL4, RMCE, protein tagging, Trojan exons, swappable insertion cassette (SIC), Nmnat"
  ], 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.7554/eLife.38709", 
  "@id": "https://doi.org/10.7554/eLife.38709", 
  "@type": "PresentationDigitalDocument", 
  "name": "An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila - sequence files"
}
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