Patent Open Access
Lyundup, A.V.; Dyuzheva, T.G.; Krasheninnikov, M.E.; Klabukov, I.D.
SUBSTANCE: present invention relates to medicine and can be used to produce a tissue engineering construct. To do this, a fragment of native skin obtained by biopsy, resection, removal of the organ and aspiration, is placed under sterile conditions in a container with a transport medium, a piece of tissue outside the body is placed in a complete nutrient medium consisting of the complete DMEM/F12 culture medium and 10 % fetal bovine serum at the bottom of the Petri dish with the dermal side up, the latter connected to a membrane of biocompatible material.
EFFECT: invention provides tissue engineering structures with inclusion of skin cells for which the in vitro cultivation stage is undesirable. 1 cl, 1 dwg, 1 ex