Dataset Open Access

Large scale expression and purification of huntingtin fragment A667-C3140 from baculoviral expression system production in sf9 insect cells – 2018/08/02

Harding, Rachel; Lin, Linda; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled

Project - Huntingtin structure-function open lab notebook. 

Rationale: To obtain monodisperse and conformationally constrained huntingtin protein samples for high resolution structural biology.

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. This project is funded and supported with generous funding from the Huntington Society of Canada and the CHDI Foundation. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
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