SPIDER-em (Electron microscopy software) SPIDER and Web Distribution Version: 24.08

SPIDER (System for Processing Image Data from Electron microscopy and Related fields) is an image processing system for electron microscopy.

• Emphasis on:
  • Averaging of single particle macromoleculer specimens
  • 3D reconstruction
  • Multivariate statistical analysis
  • Object segmentation from volumes
  • Electron tomography.
• Features:
  • Interactive command line interface.
  • Hierarchical modular design for scripting.
  • Graphical User Interface, Web, for visualizing and interacting with images.
  • File format interchangeable with other scientific imaging systems.
  • Extensive documentation of operations and techniques.
  • Includes executables and source code for use on Linux.
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• History:
  • Originated in 1978 at the Wadsworth Center in Albany, NY by Joachim Frank who shared the 2017 Nobel Prize in Chemistry for his pioneering cryo-em research done at the Wadsworth Center in Albany.
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  • Contributors (in chronological order): J. Frank, B. Shimkin, H. Dowse, L. Miranda, C. Mannella, J. P. Bretaudiere, M. V. Heel, A. Verschoor, M. Radermacher, A. Leith, J. M. Carazo, P. Penczek, L. Odesanya, Y. H. Li, M. Ladjadj, Y. Chen, K. R. Lata, J. Zhu, W. P. Liu, B. Rath, C. Yang, B. Baxter, R. Hegerl, A. Frangakis, L. Joyeux, Z. Huang, R. J. Renka, T. Shaikh, J. Sengupta, J. LeBarron, N. Boisset, H. Gao, G. Kishchenko, J. M. Kennedy.
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  • Maintained since 2007 by: ArDean Leith & Tanvir Shaikh. During these 11 years they added numerous operations, procedures, parallelizations, and over 500 pages of documentation.  There have been no significant contributors other than Leith, Shaikh, Kischenko, and Kennedy since 2007.