1326453
doi
10.5281/zenodo.1326453
oai:zenodo.org:1326453
Braun-Kiewnick, Andrea
Agroscope, Wädenswil, Switzerland
Viaene, Nicole
Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium
Hallmann, Johannes
Julius Kühn-Institut (JKI), Münster, Germany
Niere, Bkörn
Julius Kühn-Institut (JKI), Braunchsweig, Germany
Gamel, Sylvie
French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Le Rheu, France
Roversi, Pio Federico
Agriculture Research Council (CRA), Firenze, Italy
den Nijs, Loes
National Plant Protection Organization (NVWA), Wageningen, The Netherlands
van de Vossenberg, Bart
National Plant Protection Organization (NVWA), Wageningen, The Netherlands
Toktay, Halil
University of Nigde, ?igde, Turkey
Prior, Tom
The Food & Environment Research Agency (FERA), Sand Hutton, United Kingdom
Sapp, Melanie
The Food & Environment Research Agency (FERA), Sand Hutton, United Kingdom
Development and validation of innovative diagnostic tools for detection and identification of the quarantine nematode Meloidogyne enterolobii in support of integrated plant protection strategies in the EU member states
Kiewnick, Sebastian
Agroscope, Wädenswil, Switzerland
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
Euphresco, plant health, diagnostics, test performance study, Meloidogyne enterolobii
<p>The aim of this Euphresco project was to coordinate ongoing and future research in the field of Meloidogyne enterolobii detection and identification and to validate new methods in a collaborative way. It contained six main topics:</p>
<p>- Test performance study on nematode extraction methods to detect motile stages (second stage juveniles) of M. enterolobii in soil samples.</p>
<p>- Test performance study on fast and simple DNA extraction from target nematode in complex nematode suspension after extraction from soil samples (Lysis buffer; Holterman et al., 2009). DNA needs to be clean enough (without inhibitors) for further by molecular detection/identification tools. Labs were encouraged but not required to use additional extraction methods. The aim was to test a fast and simple DNA extraction protocol that can be harmonized between NPPO laboratories in the future.</p>
<p>- Methods development and in-house validation of qPCR methods to detect, identify and potentially quantify M. enterolobii in complex nematode suspensions obtained from soil samples (develop protocols for robust, specific and sensitive detection/identification that can be harmonized between NPPO labs, independent of lab equipment and chemistry).</p>
<p>- Test performance study on protocols developed under topic 3.</p>
<p>- Final meeting to communicate and discuss results. Optional workshop on Barcoding as new diagnostic tool for fast and reliable nematode identification was offered to interested groups.</p>
<p>- Publication of results in peer-reviewed research journals and discussion for inclusion of protocols into EPPO diagnostic standard protocol. Identification of future research priorities in the field of Meloidogyne enterolobii research.</p>
Scientific report of the Euphresco funded project 'Development and validation of innovative diagnostic tools for detection and identification of the quarantine nematode Meloidogyne enterolobii in support of integrated plant protection strategies in the EU member states'
Zenodo
2013-12-31
info:eu-repo/semantics/report
1326452
1579535439.692695
1019273
md5:cdfa6363caf363e7ba23576f943c0bb5
https://zenodo.org/records/1326453/files/meloid_final_report.pdf
public
10.5281/zenodo.1326452
isVersionOf
doi