Report Open Access

Development and validation of innovative diagnostic tools for detection and identification of the quarantine nematode Meloidogyne enterolobii in support of integrated plant protection strategies in the EU member states

Kiewnick, Sebastian; Braun-Kiewnick, Andrea; Viaene, Nicole; Hallmann, Johannes; Niere, Bkörn; Gamel, Sylvie; Roversi, Pio Federico; den Nijs, Loes; van de Vossenberg, Bart; Toktay, Halil; Prior, Tom; Sapp, Melanie


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="DOI">10.5281/zenodo.1326453</identifier>
  <creators>
    <creator>
      <creatorName>Kiewnick, Sebastian</creatorName>
      <givenName>Sebastian</givenName>
      <familyName>Kiewnick</familyName>
      <affiliation>Agroscope, Wädenswil, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Braun-Kiewnick, Andrea</creatorName>
      <givenName>Andrea</givenName>
      <familyName>Braun-Kiewnick</familyName>
      <affiliation>Agroscope, Wädenswil, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Viaene, Nicole</creatorName>
      <givenName>Nicole</givenName>
      <familyName>Viaene</familyName>
      <affiliation>Institute for Agricultural and Fisheries Research (ILVO), Merelbeke, Belgium</affiliation>
    </creator>
    <creator>
      <creatorName>Hallmann, Johannes</creatorName>
      <givenName>Johannes</givenName>
      <familyName>Hallmann</familyName>
      <affiliation>Julius Kühn-Institut (JKI), Münster, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Niere, Bkörn</creatorName>
      <givenName>Bkörn</givenName>
      <familyName>Niere</familyName>
      <affiliation>Julius Kühn-Institut (JKI), Braunchsweig, Germany</affiliation>
    </creator>
    <creator>
      <creatorName>Gamel, Sylvie</creatorName>
      <givenName>Sylvie</givenName>
      <familyName>Gamel</familyName>
      <affiliation>French Agency for Food, Environmental and Occupational Health &amp; Safety (ANSES), Le Rheu, France</affiliation>
    </creator>
    <creator>
      <creatorName>Roversi, Pio Federico</creatorName>
      <givenName>Pio Federico</givenName>
      <familyName>Roversi</familyName>
      <affiliation>Agriculture Research Council (CRA), Firenze, Italy</affiliation>
    </creator>
    <creator>
      <creatorName>den Nijs, Loes</creatorName>
      <givenName>Loes</givenName>
      <familyName>den Nijs</familyName>
      <affiliation>National Plant Protection Organization (NVWA), Wageningen, The Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>van de Vossenberg, Bart</creatorName>
      <givenName>Bart</givenName>
      <familyName>van de Vossenberg</familyName>
      <affiliation>National Plant Protection Organization (NVWA), Wageningen, The Netherlands</affiliation>
    </creator>
    <creator>
      <creatorName>Toktay, Halil</creatorName>
      <givenName>Halil</givenName>
      <familyName>Toktay</familyName>
      <affiliation>University of Nigde, ?igde, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Prior, Tom</creatorName>
      <givenName>Tom</givenName>
      <familyName>Prior</familyName>
      <affiliation>The Food &amp; Environment Research Agency (FERA), Sand Hutton, United Kingdom</affiliation>
    </creator>
    <creator>
      <creatorName>Sapp, Melanie</creatorName>
      <givenName>Melanie</givenName>
      <familyName>Sapp</familyName>
      <affiliation>The Food &amp; Environment Research Agency (FERA), Sand Hutton, United Kingdom</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Development and validation of innovative diagnostic tools for detection and identification of the quarantine nematode Meloidogyne enterolobii in support of integrated plant protection strategies in the EU member states</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2013</publicationYear>
  <subjects>
    <subject>Euphresco, plant health, diagnostics, test performance study, Meloidogyne enterolobii</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2013-12-31</date>
  </dates>
  <language>en</language>
  <resourceType resourceTypeGeneral="Report"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1326453</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1326452</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;The aim of this Euphresco project was to coordinate ongoing and future research in the field of Meloidogyne enterolobii detection and identification and to validate new methods in a collaborative way. It contained six main topics:&lt;/p&gt;

&lt;p&gt;- Test performance study on nematode extraction methods to detect motile stages (second stage juveniles) of M. enterolobii in soil samples.&lt;/p&gt;

&lt;p&gt;- Test performance study on fast and simple DNA extraction from target nematode in complex nematode suspension after extraction from soil samples (Lysis buffer; Holterman et al., 2009). DNA needs to be clean enough (without inhibitors) for further by molecular detection/identification tools. Labs were encouraged but not required to use additional extraction methods. The aim was to test a fast and simple DNA extraction protocol that can be harmonized between NPPO laboratories in the future.&lt;/p&gt;

&lt;p&gt;- Methods development and in-house validation of qPCR methods to detect, identify and potentially quantify M. enterolobii in complex nematode suspensions obtained from soil samples (develop protocols for robust, specific and sensitive detection/identification that can be harmonized between NPPO labs, independent of lab equipment and chemistry).&lt;/p&gt;

&lt;p&gt;- Test performance study on protocols developed under topic 3.&lt;/p&gt;

&lt;p&gt;- Final meeting to communicate and discuss results. Optional workshop on Barcoding as new diagnostic tool for fast and reliable nematode identification was offered to interested groups.&lt;/p&gt;

&lt;p&gt;- Publication of results in peer-reviewed research journals and discussion for inclusion of protocols into EPPO diagnostic standard protocol. Identification of future research priorities in the field of Meloidogyne enterolobii research.&lt;/p&gt;</description>
    <description descriptionType="Other">Scientific report of the Euphresco funded project 'Development and validation of innovative diagnostic tools for detection and identification of the quarantine nematode Meloidogyne enterolobii in support of integrated plant protection strategies in the EU member states'</description>
  </descriptions>
</resource>
165
114
views
downloads
All versions This version
Views 165165
Downloads 114114
Data volume 116.2 MB116.2 MB
Unique views 148148
Unique downloads 105105

Share

Cite as