July 23, 2018 Presentation Open Access

Bittremieux, Wout; Meysman, Pieter; Noble, William Stafford; Laukens, Kris

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    "description": "<p>Open modification search (OMS) is a powerful search strategy that identifies peptides carrying any type of modification by allowing a modified spectrum to match against its unmodified variant by using a very wide precursor mass window. A drawback of this strategy, however, is that it leads to a large increase in search time. Although performing an open search can be done using existing spectral library search engines by simply setting a wide precursor mass window, none of these tools have been optimized for OMS, leading to excessive runtimes and suboptimal identification results.</p>\n\n<p>Here we present the ANN-SoLo tool for fast and accurate open spectral library searching. ANN-SoLo uses approximate nearest neighbor indexing to speed up OMS by selecting only a limited number of the most relevant library spectra to compare to an unknown query spectrum. This approach is combined with a cascade search strategy to maximize the number of identified unmodified and modified spectra while strictly controlling the false discovery rate, as well as a shifted dot product score to sensitively match modified spectra to their unmodified counterparts.</p>\n\n<p>ANN-SoLo outperforms the state-of-the-art SpectraST spectral library search engine both in terms of speed and the number of identifications. On a previously published human cell line data set, ANN-SoLo confidently identifies 40% more spectra than SpectraST while achieving a speedup of an order of magnitude.</p>\n\n<p>ANN-SoLo is implemented in Python and C++. It is freely available under the Apache 2.0 license at https://github.com/bittremieux/ANN-SoLo.</p>", 
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    "title": "Fast open modification spectral library searching through approximate nearest neighbor indexing", 
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        "affiliation": "University of Washington", 
        "name": "Bittremieux, Wout"
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        "orcid": "0000-0001-5903-633X", 
        "affiliation": "University of Antwerp", 
        "name": "Meysman, Pieter"
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        "orcid": "0000-0001-7283-4715", 
        "affiliation": "University of Washington", 
        "name": "Noble, William Stafford"
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        "orcid": "0000-0002-8217-2564", 
        "affiliation": "University of Antwerp", 
        "name": "Laukens, Kris"
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    "meeting": {
      "url": "http://www.cascadiaproteomics.org/", 
      "dates": "23-24 July 2018", 
      "place": "Seattle, WA, USA", 
      "title": "Cascadia Proteomics Symposium"
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