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Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28

Harding, Rachel; Seitova, Alma; Hutchinson, Ashley; Arrowsmith, Cheryl; Edwards, Aled


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  "@id": "https://doi.org/10.5281/zenodo.1305940", 
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      "@id": "https://orcid.org/0000-0002-1134-391X", 
      "@type": "Person", 
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "name": "Harding, Rachel"
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      "@type": "Person", 
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "name": "Seitova, Alma"
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      "@type": "Person", 
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "name": "Hutchinson, Ashley"
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      "@type": "Person", 
      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "name": "Arrowsmith, Cheryl"
    }, 
    {
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      "affiliation": "Structural Genomics Consortium, University of Toronto", 
      "name": "Edwards, Aled"
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  "datePublished": "2018-05-28", 
  "description": "<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook.&nbsp;</p>\n\n<p><strong>Experiment and rationale</strong>&nbsp;-&nbsp;Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&nbsp;</p>", 
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  "identifier": "https://doi.org/10.5281/zenodo.1305940", 
  "keywords": [
    "Huntingtin", 
    "HTT", 
    "HAP40", 
    "Protein-protein interaction", 
    "SAXS"
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  "license": "http://creativecommons.org/licenses/by/4.0/legalcode", 
  "name": "Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells \u2013 2018/05/28", 
  "url": "https://zenodo.org/record/1305940"
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