Dataset Open Access

Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28

Harding, Rachel; Seitova, Alma; Hutchinson, Ashley; Arrowsmith, Cheryl; Edwards, Aled


DataCite XML Export

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="DOI">10.5281/zenodo.1305940</identifier>
  <creators>
    <creator>
      <creatorName>Harding, Rachel</creatorName>
      <givenName>Rachel</givenName>
      <familyName>Harding</familyName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-1134-391X</nameIdentifier>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Seitova, Alma</creatorName>
      <givenName>Alma</givenName>
      <familyName>Seitova</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Hutchinson, Ashley</creatorName>
      <givenName>Ashley</givenName>
      <familyName>Hutchinson</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Arrowsmith, Cheryl</creatorName>
      <givenName>Cheryl</givenName>
      <familyName>Arrowsmith</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Edwards, Aled</creatorName>
      <givenName>Aled</givenName>
      <familyName>Edwards</familyName>
      <affiliation>Structural Genomics Consortium, University of Toronto</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <subjects>
    <subject>Huntingtin</subject>
    <subject>HTT</subject>
    <subject>HAP40</subject>
    <subject>Protein-protein interaction</subject>
    <subject>SAXS</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2018-05-28</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1305940</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1305939</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/labscribbles</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/openlabnotebooks</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/sgc-opennotebook</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;&lt;strong&gt;Project&lt;/strong&gt;&amp;nbsp;- Huntingtin structure-function open lab notebook.&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Experiment and rationale&lt;/strong&gt;&amp;nbsp;-&amp;nbsp;Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&amp;nbsp;&lt;/p&gt;</description>
    <description descriptionType="Other">Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</description>
  </descriptions>
</resource>
49
32
views
downloads
All versions This version
Views 4949
Downloads 3232
Data volume 42.0 MB42.0 MB
Unique views 3939
Unique downloads 2121

Share

Cite as