Dataset Open Access

Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28

Harding, Rachel; Seitova, Alma; Hutchinson, Ashley; Arrowsmith, Cheryl; Edwards, Aled


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{
  "publisher": "Zenodo", 
  "DOI": "10.5281/zenodo.1305940", 
  "title": "Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells \u2013 2018/05/28", 
  "issued": {
    "date-parts": [
      [
        2018, 
        5, 
        28
      ]
    ]
  }, 
  "abstract": "<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook.&nbsp;</p>\n\n<p><strong>Experiment and rationale</strong>&nbsp;-&nbsp;Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&nbsp;</p>", 
  "author": [
    {
      "family": "Harding, Rachel"
    }, 
    {
      "family": "Seitova, Alma"
    }, 
    {
      "family": "Hutchinson, Ashley"
    }, 
    {
      "family": "Arrowsmith, Cheryl"
    }, 
    {
      "family": "Edwards, Aled"
    }
  ], 
  "note": "Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, S\u00e3o Paulo Research Foundation-FAPESP, Takeda, and Wellcome.", 
  "type": "dataset", 
  "id": "1305940"
}
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