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Structure of Complement C3(H2O) Revealed By Quantitative Cross-Linking/Mass Spectrometry And Modeling

Chen, ZA; Pellarin, R; Fischer, L; Sali, A; Nilges, M; Barlow, PN; Rappsilber, J

Data manager(s)
Webb, Benjamin

The slow but spontaneous and ubiquitous formation of C3(H2O), the hydrolytic and conformationally rearranged product of C3, initiates antibody-independent activation of the complement system that is a key first line of antimicrobial defense. The structure of C3(H2O) has not been determined. Here we subjected C3(H2O) to quantitative cross-linking/mass spectrometry (QCLMS). This revealed details of the structural differences and similarities between C3(H2O) and C3, as well as between C3(H2O) and its pivotal proteolytic cleavage product, C3b, which shares functionally similarity with C3(H2O). Considered in combination with the crystal structures of C3 and C3b, the QCMLS data suggest that C3(H2O) generation is accompanied by the migration of the thioester-containing domain of C3 from one end of the molecule to the other. This creates a stable C3b-like platform able to bind the zymogen, factor B, or the regulator, factor H. Integration of available crystallographic and QCLMS data allowed the determination of a 3D model of the C3(H2O) domain architecture. The unique arrangement of domains thus observed in C3(H2O), which retains the anaphylatoxin domain (that is excised when C3 is enzymatically activated to C3b), can be used to rationalize observed differences between C3(H2O) and C3b in terms of complement activation and regulation.

For more information about how to reproduce this modeling, see the Sali lab website or the README file.

Files (1.6 GB)
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c3-cluster0.dcd
md5:d2cb2d0ba0e6f828d3fb1de56d5dc251
3.9 MB Download
c3b-cluster0.dcd
md5:6238a96f15cad2f4674bbcc1b4b7a888
3.7 MB Download
ic3-cluster0.dcd
md5:321d6c3b0d2473b254d25ebc7b6ee1bb
1.8 MB Download
ic3-cluster1.dcd
md5:ab04fd31a21a885e95dc8c06a19490e4
2.2 MB Download
integrativemodeling/Complement-v1.0.zip
md5:0b5ff88f1333335f4e9754ce25db54ab
74.7 MB Download
traj-c3.1.tar.xz
md5:3c075e561094c7d696040358430c39d2
247.5 MB Download
traj-c3.2.tar.xz
md5:388c9c7d5acf78229b5230dd98ea9316
247.6 MB Download
traj-c3b.1.tar.xz
md5:d11323e7a8d26ae10c63be51f8bf755f
248.5 MB Download
traj-c3b.2.tar.xz
md5:2081c8767cfccdd10e1cb76f732fe816
245.5 MB Download
traj-ic3.1.tar.xz
md5:a114ac2eb0b0ca52d7ad447046f1f7b2
260.5 MB Download
traj-ic3.2.tar.xz
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260.5 MB Download
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