Dataset Open Access

The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud


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    <subfield code="a">&lt;p&gt;With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.&lt;/p&gt;

&lt;p&gt;&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Note: &lt;/strong&gt;1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).&lt;/p&gt;

&lt;p&gt;&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.&lt;/p&gt;</subfield>
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