1270383
doi
10.5281/zenodo.1270383
oai:zenodo.org:1270383
user-sgc-opennotebook
user-openlabnotebooks
Vedadi, Masoud
Structural Genomics Consortium/University of Toronto
The process of HDAC11 Assay Development: linearity and Km calculation
Abbey, Megha
Structural Genomics Consortium/University of Toronto
info:eu-repo/semantics/openAccess
Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
HDAC11
linearity
enzyme kinetics
burst
burst kinetics
boc-lys-TFA-AMC
<p>With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.</p>
<p> </p>
<p><strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).</p>
<p> 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</p>
The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Zenodo
2018-06-04
info:eu-repo/semantics/other
1270382
user-sgc-opennotebook
user-openlabnotebooks
1579893933.163939
237592
md5:ab7cfc34561393a3eb8d48991a0388ca
https://zenodo.org/records/1270383/files/4june2018-HDAC11 assay deve process-linearity-Km.pdf
public
10.5281/zenodo.1270382
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doi