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The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud


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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Abbey, Megha</dc:creator>
  <dc:creator>Vedadi, Masoud</dc:creator>
  <dc:date>2018-06-04</dc:date>
  <dc:description>With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.

 

Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).

          2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</dc:description>
  <dc:description>The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dc:description>
  <dc:identifier>https://zenodo.org/record/1270383</dc:identifier>
  <dc:identifier>10.5281/zenodo.1270383</dc:identifier>
  <dc:identifier>oai:zenodo.org:1270383</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.1270382</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:subject>HDAC11</dc:subject>
  <dc:subject>linearity</dc:subject>
  <dc:subject>enzyme kinetics</dc:subject>
  <dc:subject>burst</dc:subject>
  <dc:subject>burst kinetics</dc:subject>
  <dc:subject>boc-lys-TFA-AMC</dc:subject>
  <dc:title>The process of HDAC11 Assay Development: linearity and Km calculation</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
</oai_dc:dc>
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