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The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud

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<oai_dc:dc xmlns:dc="" xmlns:oai_dc="" xmlns:xsi="" xsi:schemaLocation="">
  <dc:creator>Abbey, Megha</dc:creator>
  <dc:creator>Vedadi, Masoud</dc:creator>
  <dc:description>With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.


Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).

          2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</dc:description>
  <dc:description>The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dc:description>
  <dc:subject>enzyme kinetics</dc:subject>
  <dc:subject>burst kinetics</dc:subject>
  <dc:title>The process of HDAC11 Assay Development: linearity and Km calculation</dc:title>
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