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The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud


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{
  "description": "<p>With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.</p>\n\n<p>&nbsp;</p>\n\n<p><strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).</p>\n\n<p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium/University of Toronto", 
      "@type": "Person", 
      "name": "Abbey, Megha"
    }, 
    {
      "affiliation": "Structural Genomics Consortium/University of Toronto", 
      "@type": "Person", 
      "name": "Vedadi, Masoud"
    }
  ], 
  "url": "https://zenodo.org/record/1270383", 
  "datePublished": "2018-06-04", 
  "keywords": [
    "HDAC11", 
    "linearity", 
    "enzyme kinetics", 
    "burst", 
    "burst kinetics", 
    "boc-lys-TFA-AMC"
  ], 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/53f70920-e68c-4976-83cc-31938a6bf02e/4june2018-HDAC11 assay deve process-linearity-Km.pdf", 
      "encodingFormat": "pdf", 
      "@type": "DataDownload"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.1270383", 
  "@id": "https://doi.org/10.5281/zenodo.1270383", 
  "@type": "Dataset", 
  "name": "The process of HDAC11 Assay Development: linearity and Km calculation"
}
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