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The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud


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    "description": "<p>With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.</p>\n\n<p>&nbsp;</p>\n\n<p><strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).</p>\n\n<p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</p>", 
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    "title": "The process of HDAC11 Assay Development: linearity and Km calculation", 
    "notes": "The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, S\u00e3o Paulo Research Foundation-FAPESP, Takeda, and Wellcome.", 
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    "keywords": [
      "HDAC11", 
      "linearity", 
      "enzyme kinetics", 
      "burst", 
      "burst kinetics", 
      "boc-lys-TFA-AMC"
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    "publication_date": "2018-06-04", 
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        "name": "Abbey, Megha"
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