June 4, 2018 Dataset Open Access

Abbey, Megha; Vedadi, Masoud

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  <identifier identifierType="DOI">10.5281/zenodo.1270383</identifier>
  <creators>
    <creator>
      <creatorName>Abbey, Megha</creatorName>
      <givenName>Megha</givenName>
      <familyName>Abbey</familyName>
      <affiliation>Structural Genomics Consortium/University of Toronto</affiliation>
    </creator>
    <creator>
      <creatorName>Vedadi, Masoud</creatorName>
      <givenName>Masoud</givenName>
      <familyName>Vedadi</familyName>
      <affiliation>Structural Genomics Consortium/University of Toronto</affiliation>
    </creator>
  </creators>
  <titles>
    <title>The process of HDAC11 Assay Development: linearity and Km calculation</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <subjects>
    <subject>HDAC11</subject>
    <subject>linearity</subject>
    <subject>enzyme kinetics</subject>
    <subject>burst</subject>
    <subject>burst kinetics</subject>
    <subject>boc-lys-TFA-AMC</subject>
  </subjects>
  <dates>
    <date dateType="Issued">2018-06-04</date>
  </dates>
  <resourceType resourceTypeGeneral="Dataset"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1270383</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1270382</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/openlabnotebooks</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsPartOf">https://zenodo.org/communities/sgc-opennotebook</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="https://creativecommons.org/licenses/by/4.0/legalcode">Creative Commons Attribution 4.0 International</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.&lt;/p&gt;

&lt;p&gt;&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Note: &lt;/strong&gt;1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).&lt;/p&gt;

&lt;p&gt;&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.&lt;/p&gt;</description>
    <description descriptionType="Other">The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</description>
  </descriptions>
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