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The process of HDAC11 Assay Development: linearity and Km calculation

Abbey, Megha; Vedadi, Masoud


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    <dct:title>The process of HDAC11 Assay Development: linearity and Km calculation</dct:title>
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    <dct:issued rdf:datatype="http://www.w3.org/2001/XMLSchema#gYear">2018</dct:issued>
    <dcat:keyword>HDAC11</dcat:keyword>
    <dcat:keyword>linearity</dcat:keyword>
    <dcat:keyword>enzyme kinetics</dcat:keyword>
    <dcat:keyword>burst</dcat:keyword>
    <dcat:keyword>burst kinetics</dcat:keyword>
    <dcat:keyword>boc-lys-TFA-AMC</dcat:keyword>
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    <dct:description>&lt;p&gt;With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated.&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Note: &lt;/strong&gt;1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).&lt;/p&gt; &lt;p&gt;&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.&lt;/p&gt;</dct:description>
    <dct:description>The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dct:description>
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