Dataset Open Access

Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells

Dilworth, David; Barsyte-Lovejoy, Dalia


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  <dc:creator>Dilworth, David</dc:creator>
  <dc:creator>Barsyte-Lovejoy, Dalia</dc:creator>
  <dc:date>2018-05-23</dc:date>
  <dc:description>SGC Open Notebook Project to Characterize the HMTase NSD3

Exp023 Objective: Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting.  </dc:description>
  <dc:description>Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.</dc:description>
  <dc:identifier>https://zenodo.org/record/1263398</dc:identifier>
  <dc:identifier>10.5281/zenodo.1263398</dc:identifier>
  <dc:identifier>oai:zenodo.org:1263398</dc:identifier>
  <dc:relation>doi:10.5281/zenodo.1251838</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/openlabnotebooks</dc:relation>
  <dc:relation>url:https://zenodo.org/communities/sgc-opennotebook</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/licenses/by/4.0/legalcode</dc:rights>
  <dc:title>Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells</dc:title>
  <dc:type>info:eu-repo/semantics/other</dc:type>
  <dc:type>dataset</dc:type>
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