Dataset Open Access

Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells

Dilworth, David; Barsyte-Lovejoy, Dalia


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{
  "description": "<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p>\n\n<p><strong>Exp023 Objective:&nbsp;</strong>Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting. &nbsp;</p>", 
  "license": "https://creativecommons.org/licenses/by/4.0/legalcode", 
  "creator": [
    {
      "affiliation": "Structural Genomics Consortium", 
      "@id": "https://orcid.org/0000-0001-5557-9476", 
      "@type": "Person", 
      "name": "Dilworth, David"
    }, 
    {
      "affiliation": "Structural Genomics Consortium", 
      "@type": "Person", 
      "name": "Barsyte-Lovejoy, Dalia"
    }
  ], 
  "url": "https://zenodo.org/record/1263398", 
  "datePublished": "2018-05-23", 
  "@context": "https://schema.org/", 
  "distribution": [
    {
      "contentUrl": "https://zenodo.org/api/files/0cac784c-a17e-4b1e-b5ea-6a5c778169c7/exp023.pdf", 
      "encodingFormat": "pdf", 
      "@type": "DataDownload"
    }, 
    {
      "contentUrl": "https://zenodo.org/api/files/0cac784c-a17e-4b1e-b5ea-6a5c778169c7/exp023.tar.gz", 
      "encodingFormat": "gz", 
      "@type": "DataDownload"
    }
  ], 
  "identifier": "https://doi.org/10.5281/zenodo.1263398", 
  "@id": "https://doi.org/10.5281/zenodo.1263398", 
  "@type": "Dataset", 
  "name": "Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells"
}
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