Dataset Open Access

Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells

Dilworth, David; Barsyte-Lovejoy, Dalia


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    "description": "<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p>\n\n<p><strong>Exp023 Objective:&nbsp;</strong>Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting. &nbsp;</p>", 
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    "title": "Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells", 
    "notes": "Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, S\u00e3o Paulo Research Foundation-FAPESP, Takeda, and Wellcome.", 
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