10.5281/zenodo.1254295
https://zenodo.org/records/1254295
oai:zenodo.org:1254295
Carlsson, Jeanette
Jeanette
Carlsson
University College Dublin, Belfield, Dublin
Carlsson, Jens
Jens
Carlsson
0000-0002-9262-5627
University College Dublin, Belfield, Dublin
Carreiro-Silva, Marina
Marina
Carreiro-Silva
0000-0001-6446-6342
Marine Laboratory, Marine Scotland Science, United Kingdom 7IMAR - Institute of Marine Research, University of the Azores, Portugal
Morato, Telmo
Telmo
Morato
0000-0003-2393-4773
Marine Laboratory, Marine Scotland Science, United Kingdom 7IMAR - Institute of Marine Research, University of the Azores, Portugal
Brandt, Miriam
Miriam
Brandt
IFREMER - UMR MARBEC, Sète-Montpellier, France
Orejas, Covadonga
Covadonga
Orejas
0000-0002-2580-1002
Centro Oceanográfico de Baleres, Instituto Español de Oceanografía
Arnaud-Haond, Sophie
Sophie
Arnaud-Haond
0000-0001-5814-8452
IFREMER - UMR MARBEC, Sète-Montpellier, France
Improving biodiversity inventories based on metabarcoding of eDNA
Zenodo
2018
2018-05-28
Presentation
10.5281/zenodo.1254294
https://zenodo.org/communities/atlas
https://zenodo.org/communities/eu
Creative Commons Attribution 4.0 International
ATLAS work package 4 presentation at ATLAS 3rd General Assembly
In the framework of ATLAS, we planned to sample sediment and water to test our ability to produce biodiversity inventories based on environmental DNA. During Medwaves cruise, we sampled sediment from four sites spanning from the Mediterranean to the Azores, with the objective to characterize molecularly the communities associated to cold-water corals in those four site and study the evolution as the Mediterranean Outflow Water penetrates the Atlantic. We sampled sediment with multicorers, and water with niskin bottles on the seafloor and CTD rosettes in the water column. We proceeded to molecular tests (RNA, DNA, and DNA treated to avoid extracellular DNA) to design the best molecular and bioinformatic pipelines allowing an optimal characterization of prokaryotic and eukaryotic communities. Preliminary results from this work will be discussed.
European Commission
10.13039/501100000780
678760
A Trans-AtLantic Assessment and deep-water ecosystem-based Spatial management plan for Europe