June 20, 2018 Journal article Open Access

Habib Chouchane

MARC21 XML Export

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    <subfield code="u">https://zenodo.org/record/1253982/files/Accepted manuscript Habib Chouchane, Mouna Mahjoubi, Besma Ettoumi, Mohamed Neifar &amp; Ameur Cherif (2017) A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S an.pdf</subfield>
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    <subfield code="a">A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S and its application in dye removal</subfield>
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    <subfield code="a">&lt;p&gt;A new bioflocculant named pKr produced by hydrocarbonoclastic strain Kocuria rosea BU22S&lt;br&gt;
(KC152976) was investigated. Gas chromatography&amp;ndash;flame ionization detector (GC-FID) analysis&lt;br&gt;
confirmed the high potential of the strain BU22S in the degradation of n-alkanes. Plackett&amp;ndash;&lt;br&gt;
Burman experimental design and response surface methodology were carried out to optimize&lt;br&gt;
pKr production. Glucose, peptone and incubation time were found to be the most significant&lt;br&gt;
factors affecting bioflocculant production. Maximum pKr production was about 4.72 &amp;plusmn; 0.02 g/L&lt;br&gt;
achieved with 15.61 g/L glucose, 6.45 g/L peptone and 3 days incubation time. Chemical analysis&lt;br&gt;
of pKr indicated that it contained 71.62% polysaccharides, 16.36% uronic acid and 2.83%&lt;br&gt;
proteins. Thin layer chromatography analysis showed that polysaccharides fraction consisted of&lt;br&gt;
galactose and xylose. Fourier transform infrared analysis revealed the presence of many&lt;br&gt;
functional groups, hydroxyl, carboxyl, methoxyl, acetyl and amide that likely contribute to&lt;br&gt;
flocculation. K. rosea pKr showed high flocculant potential using kaolin clay at different pH (2&amp;ndash;&lt;br&gt;
11), temperature (0&amp;ndash;100&amp;deg;C) and cation concentrations. The bioflocculant was particularly effective&lt;br&gt;
in flocculating soluble anionic dyes, Reactive Blue 4 and Acid Yellow, with a decolorization&lt;br&gt;
efficiency of 76.4% and 72.6%, respectively. The outstanding flocculating performances suggest&lt;br&gt;
that pKr could be useful for bioremediation applications.&lt;/p&gt;</subfield>
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