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A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S and its application in dye removal

Habib Chouchane


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    "description": "<p>A new bioflocculant named pKr produced by hydrocarbonoclastic strain Kocuria rosea BU22S<br>\n(KC152976) was investigated. Gas chromatography&ndash;flame ionization detector (GC-FID) analysis<br>\nconfirmed the high potential of the strain BU22S in the degradation of n-alkanes. Plackett&ndash;<br>\nBurman experimental design and response surface methodology were carried out to optimize<br>\npKr production. Glucose, peptone and incubation time were found to be the most significant<br>\nfactors affecting bioflocculant production. Maximum pKr production was about 4.72 &plusmn; 0.02 g/L<br>\nachieved with 15.61 g/L glucose, 6.45 g/L peptone and 3 days incubation time. Chemical analysis<br>\nof pKr indicated that it contained 71.62% polysaccharides, 16.36% uronic acid and 2.83%<br>\nproteins. Thin layer chromatography analysis showed that polysaccharides fraction consisted of<br>\ngalactose and xylose. Fourier transform infrared analysis revealed the presence of many<br>\nfunctional groups, hydroxyl, carboxyl, methoxyl, acetyl and amide that likely contribute to<br>\nflocculation. K. rosea pKr showed high flocculant potential using kaolin clay at different pH (2&ndash;<br>\n11), temperature (0&ndash;100&deg;C) and cation concentrations. The bioflocculant was particularly effective<br>\nin flocculating soluble anionic dyes, Reactive Blue 4 and Acid Yellow, with a decolorization<br>\nefficiency of 76.4% and 72.6%, respectively. The outstanding flocculating performances suggest<br>\nthat pKr could be useful for bioremediation applications.</p>", 
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