June 20, 2018 Journal article Open Access

Habib Chouchane

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  <identifier identifierType="DOI">10.5281/zenodo.1253982</identifier>
  <creators>
    <creator>
      <creatorName>Habib Chouchane</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org/">0000-0002-6310-3758</nameIdentifier>
      <affiliation>UMA</affiliation>
    </creator>
  </creators>
  <titles>
    <title>A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S and its application in dye removal</title>
  </titles>
  <publisher>Zenodo</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-06-20</date>
  </dates>
  <resourceType resourceTypeGeneral="JournalArticle"/>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1253982</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="URL" relationType="IsSupplementedBy">https://zenodo.org/deposit/1253980</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.5281/zenodo.1253981</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;A new bioflocculant named pKr produced by hydrocarbonoclastic strain Kocuria rosea BU22S&lt;br&gt;
(KC152976) was investigated. Gas chromatography&amp;ndash;flame ionization detector (GC-FID) analysis&lt;br&gt;
confirmed the high potential of the strain BU22S in the degradation of n-alkanes. Plackett&amp;ndash;&lt;br&gt;
Burman experimental design and response surface methodology were carried out to optimize&lt;br&gt;
pKr production. Glucose, peptone and incubation time were found to be the most significant&lt;br&gt;
factors affecting bioflocculant production. Maximum pKr production was about 4.72 &amp;plusmn; 0.02 g/L&lt;br&gt;
achieved with 15.61 g/L glucose, 6.45 g/L peptone and 3 days incubation time. Chemical analysis&lt;br&gt;
of pKr indicated that it contained 71.62% polysaccharides, 16.36% uronic acid and 2.83%&lt;br&gt;
proteins. Thin layer chromatography analysis showed that polysaccharides fraction consisted of&lt;br&gt;
galactose and xylose. Fourier transform infrared analysis revealed the presence of many&lt;br&gt;
functional groups, hydroxyl, carboxyl, methoxyl, acetyl and amide that likely contribute to&lt;br&gt;
flocculation. K. rosea pKr showed high flocculant potential using kaolin clay at different pH (2&amp;ndash;&lt;br&gt;
11), temperature (0&amp;ndash;100&amp;deg;C) and cation concentrations. The bioflocculant was particularly effective&lt;br&gt;
in flocculating soluble anionic dyes, Reactive Blue 4 and Acid Yellow, with a decolorization&lt;br&gt;
efficiency of 76.4% and 72.6%, respectively. The outstanding flocculating performances suggest&lt;br&gt;
that pKr could be useful for bioremediation applications.&lt;/p&gt;</description>
  </descriptions>
  <fundingReferences>
    <fundingReference>
      <funderName>European Commission</funderName>
      <funderIdentifier funderIdentifierType="Crossref Funder ID">10.13039/100010661</funderIdentifier>
      <awardNumber awardURI="info:eu-repo/grantAgreement/EC/H2020/688320/">688320</awardNumber>
      <awardTitle>DevelopMent AnD application of integrated technological and management solutions FOR wasteWATER treatment and efficient reuse in agriculture tailored to the needs of Mediterranean African Countries</awardTitle>
    </fundingReference>
  </fundingReferences>
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