There is a newer version of this record available.

Dataset Open Access

Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells

Dilworth, David; Barsyte-Lovejoy, Dalia

SGC Open Notebook Project to Characterize the HMTase NSD3

Exp023 Objective: Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting.  

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Files (8.2 MB)
Name Size
exp023.pdf
md5:c50979083c46d544ee148d789c78386b
979.6 kB Download
exp023.tar.gz
md5:9545c62014c7f334727ded567439ec4a
7.2 MB Download
144
98
views
downloads
All versions This version
Views 14449
Downloads 9844
Data volume 208.1 MB93.1 MB
Unique views 10837
Unique downloads 7034

Share

Cite as