1234433
doi
10.1080/00103629509369465
oai:zenodo.org:1234433
Mulvaney, R. L.
Sims, G. K.
A microscale method for colorimetric determination of urea in soil extracts
Greenan, N. S.
info:eu-repo/semantics/openAccess
Creative Commons Zero v1.0 Universal
https://creativecommons.org/publicdomain/zero/1.0/legalcode
The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.
Zenodo
1995-09-01
info:eu-repo/semantics/article
1234432
1579541206.699831
422539
md5:a11487ffb9049c1063ecc4ea92ab8998
https://zenodo.org/records/1234433/files/article.pdf
public