Journal article Open Access

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.


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  <dc:creator>Greenan, N. S.</dc:creator>
  <dc:creator>Mulvaney, R. L.</dc:creator>
  <dc:creator>Sims, G. K.</dc:creator>
  <dc:date>1995-09-01</dc:date>
  <dc:description>The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.</dc:description>
  <dc:identifier>https://zenodo.org/record/1234433</dc:identifier>
  <dc:identifier>10.1080/00103629509369465</dc:identifier>
  <dc:identifier>oai:zenodo.org:1234433</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>https://creativecommons.org/publicdomain/zero/1.0/legalcode</dc:rights>
  <dc:title>A microscale method for colorimetric determination of urea in soil extracts</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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