Journal article Open Access

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.

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<oai_dc:dc xmlns:dc="" xmlns:oai_dc="" xmlns:xsi="" xsi:schemaLocation="">
  <dc:creator>Greenan, N. S.</dc:creator>
  <dc:creator>Mulvaney, R. L.</dc:creator>
  <dc:creator>Sims, G. K.</dc:creator>
  <dc:description>The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.</dc:description>
  <dc:title>A microscale method for colorimetric determination of urea in soil extracts</dc:title>
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