Journal article Open Access

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.


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{
  "description": "The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100\u2010\u03bcL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low\u2010temperature oven at 87\u00b0C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.", 
  "license": "https://creativecommons.org/publicdomain/zero/1.0/legalcode", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Greenan, N. S."
    }, 
    {
      "@type": "Person", 
      "name": "Mulvaney, R. L."
    }, 
    {
      "@type": "Person", 
      "name": "Sims, G. K."
    }
  ], 
  "headline": "A microscale method for colorimetric determination of urea in soil extracts", 
  "image": "https://zenodo.org/static/img/logos/zenodo-gradient-round.svg", 
  "datePublished": "1995-09-01", 
  "url": "https://zenodo.org/record/1234433", 
  "@context": "https://schema.org/", 
  "identifier": "https://doi.org/10.1080/00103629509369465", 
  "@id": "https://doi.org/10.1080/00103629509369465", 
  "@type": "ScholarlyArticle", 
  "name": "A microscale method for colorimetric determination of urea in soil extracts"
}
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