Journal article Open Access

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.

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  <identifier identifierType="URL"></identifier>
      <creatorName>Greenan, N. S.</creatorName>
      <givenName>N. S.</givenName>
      <creatorName>Mulvaney, R. L.</creatorName>
      <givenName>R. L.</givenName>
      <creatorName>Sims, G. K.</creatorName>
      <givenName>G. K.</givenName>
    <title>A microscale method for colorimetric determination of urea in soil extracts</title>
    <date dateType="Issued">1995-09-01</date>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
    <alternateIdentifier alternateIdentifierType="url"></alternateIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1080/00103629509369465</relatedIdentifier>
    <rights rightsURI="">Creative Commons Zero v1.0 Universal</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
    <description descriptionType="Abstract">The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.</description>
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