Journal article Open Access
Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="URL">https://zenodo.org/record/1234433</identifier> <creators> <creator> <creatorName>Greenan, N. S.</creatorName> <givenName>N. S.</givenName> <familyName>Greenan</familyName> </creator> <creator> <creatorName>Mulvaney, R. L.</creatorName> <givenName>R. L.</givenName> <familyName>Mulvaney</familyName> </creator> <creator> <creatorName>Sims, G. K.</creatorName> <givenName>G. K.</givenName> <familyName>Sims</familyName> </creator> </creators> <titles> <title>A microscale method for colorimetric determination of urea in soil extracts</title> </titles> <publisher>Zenodo</publisher> <publicationYear>1995</publicationYear> <dates> <date dateType="Issued">1995-09-01</date> </dates> <resourceType resourceTypeGeneral="Text">Journal article</resourceType> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://zenodo.org/record/1234433</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1080/00103629509369465</relatedIdentifier> </relatedIdentifiers> <rightsList> <rights rightsURI="https://creativecommons.org/publicdomain/zero/1.0/legalcode">Creative Commons Zero v1.0 Universal</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract">The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100‐μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low‐temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.</description> </descriptions> </resource>
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