Journal article Open Access

A microscale method for colorimetric determination of urea in soil extracts

Greenan, N. S.; Mulvaney, R. L.; Sims, G. K.


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{
  "DOI": "10.1080/00103629509369465", 
  "author": [
    {
      "family": "Greenan, N. S."
    }, 
    {
      "family": "Mulvaney, R. L."
    }, 
    {
      "family": "Sims, G. K."
    }
  ], 
  "issued": {
    "date-parts": [
      [
        1995, 
        9, 
        1
      ]
    ]
  }, 
  "abstract": "The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100\u2010\u03bcL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low\u2010temperature oven at 87\u00b0C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.", 
  "title": "A microscale method for colorimetric determination of urea in soil extracts", 
  "type": "article-journal", 
  "id": "1234433"
}
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